TW201740909A - Biological tissue long-term preservation method and device thereof employing simple and natural way to enclose biological tissue within sealed rosin for long-term preservation of biological blood or tissue samples - Google Patents

Abstract

The present invention provides a biological tissue long-term preservation method and a device thereof. The method uses a simple and natural way to enclose the biological blood or tissue with sealed rosin through packaging material, and then hardens the rosin to be gradually transformed to become amber after a long time, so as to preserve the biological blood or tissue samples for a long period. Thus, the descendant may study the biological cell and tissue samples of modern creatures, and may reproduce the modern creatures by the biotechnology at that time hundred years later and reproduce the various extinct species that could not survive due to environment destruction by human being. Thus, the present invention may realize the bio-diversity on Earth and may leave the hereditary genes to humans or creatures millions years later for studying the modern creature species and possessing absolute value, so as to understand the important information for biological evolution.

Description

Biological tissue long-term preservation method and device thereof

The present invention provides a method for long-term preservation of biological tissues and a device thereof, and particularly relates to a method for simply and naturally coating biological tissue in a sealed turpentine for long-term preservation of blood or tissue samples of biological tissues.

According to the literature, humans have been excavated or accidentally found, and rosin far from 40 million years ago is gradually petrified under good natural conditions to form amber.

Through the unearthed amber view, the interior often contains ancient insects, seeds, flowers or plant debris and other inclusions, which are vivid and clear, so it can be inferred that the biological blood or its tissue can be coated inside the resin for a long time. Preservation, allowing blood to be stored in vitro, can maintain its original integrity after seizing air and moisture for a long time.

Therefore, in view of the above-mentioned lack of blood preservation and the requirements of natural principles, the inventors have devoted themselves to observation and research using theoretical knowledge, and after many experimental improvements, finally developed a novel method for protecting blood in vitro. This method uses natural regulatory mechanisms to maintain the integrity of biological blood or its tissues, and this creation has taken place.

The purpose of the present invention is to provide a method for long-term preservation of biological tissues and a device thereof, which completely isolates the biological tissue enclosed from air and water, so that the biological tissue can be preserved for a long time without losing its integrity.

A further object of the present invention is to provide a method and apparatus for long-term preservation of biological tissues, using a simple and natural method, without adding other chemicals, without chemical residues, and without the concern of by-product residues, Biological organizations are long-term preservers.

In order to achieve the above-mentioned purpose and effect, the method adopted includes the following steps: A. A biological tissue container made of a hard material and having a circular sphere, and a hollow chamber inside, and an outer large and small inside. Conical injection port; B, in the nitrogen environment space, fresh blood is injected into the hollow chamber through the conical injection port, after the hollow chamber is filled with fresh blood, all the air in the hollow chamber is completely discharged. The conical injection port is filled with a filler, and the conical injection port is sealed with a gel to form a sealed circular container; C. The initially set turpentine is placed at the bottom of the circular mold and placed in the blood. The circular container is filled with liquid rosin to keep the circular container therein; D. After the rosin is hardened into a solid, the coating body is formed for long-term storage.

The biological tissue container is a sphere, an ellipsoid, a ring, and other suitable polygonal spheres.

The biological tissue includes blood, bone marrow, brain juice, stem cells, cells, hair, body fluids, fish, shrimp blood, insect juice, and plant juice.

The rosin further comprises a natural resin of a tree fat liquid produced by all natural pine plants.

The rosin further includes all of the synthetic chemical resins processed through the esterification process.

The turpentine further includes all of the various hardnesses and clarity of the keloid or rubber.

In order to achieve the above-mentioned purpose and effect, the method of the present invention comprises the following steps: A. a biological tissue container made of a soft material or a circular sphere, with a hollow chamber inside; B, with a syringe Fresh blood is drawn, and the needle of the syringe is directly inserted into the hollow chamber to inject fresh blood in a nitrogen environment space, and another empty needle is inserted to discharge all the air in the hollow chamber. After the hollow chamber is filled with fresh blood, the syringe is taken out. The needle and the empty needle make it directly seal itself to make it a sealed circular container; C. Place the initially set turpentine on the bottom of the circular mold, place it in a round container filled with fresh blood, and then pour it into the liquid. The turpentine holds the round container therein; D, after the rosin is hardened into a solid, the coating body is formed for long-term storage.

The biological tissue container is a sphere, an ellipsoid, a ring, and other suitable polygonal spheres.

The biological tissue includes blood, bone marrow, brain juice, stem cells, cells, hair, body fluids, fish, shrimp blood, insect juice, and plant juice.

The rosin further comprises a natural resin of a tree fat liquid produced by all natural pine plants.

The rosin further includes all of the synthetic chemical resins processed through the esterification process.

The turpentine further includes all of the various hardnesses and clarity of the keloid or rubber.

In order to achieve the above purpose and effect, the technical means adopted by the present invention comprises a circular container formed by injecting fresh blood into a biological tissue container provided with a hollow chamber; a covering body wrapped around the circle The outer circumference of the container; the circular container is covered by the covering body, so that the fresh blood in the circular container is completely isolated from air and moisture, and becomes a good long-term storage device.

The biological tissue container is made of a hard material and has a hollow spherical body with a hollow chamber, and a tapered injection port is formed on the outer circumference. After the fresh blood is injected from the tapered injection port, the tapered injection port is sealed. Forming a circular container; the biological tissue container is made of a soft material or a hollow hollow sphere having a hollow chamber. After the fresh blood is injected into the syringe, the needle of the syringe is removed, and the soft material is self-recovering and sealing. The function of forming a sealed round container.

The coating system is composed of liquid rosin hardened into a solid and converted into amber after several years.

The biological tissue container is a sphere, an ellipsoid, a ring, and other suitable polygonal spheres.

The biological tissue container is a hollow circular sphere having a diameter of 0.8 cm and a diameter of 0.4 cm.

The biological tissue includes blood, bone marrow, brain juice, stem cells, cells, hair, body fluids, fish, shrimp blood, insect juice, and plant juice.

The rosin further comprises a natural resin of a tree fat liquid produced by all natural pine plants.

The rosin further includes all of the synthetic chemical resins processed through the esterification process.

The turpentine further includes all of the various hardnesses and clarity of the keloid or rubber.

In order to gain a better understanding and understanding of the technical features and the efficacies achieved, the best examples and detailed descriptions are listed below:

According to the present invention, the biological tissues described below should at least contain blood, bone marrow, semen, brain juice, stem cells, cell tissues, body fluids (saliva, mucus), hair, and the like.

The biological organization of this creation, for the sake of simplicity of explanation, only human blood as an example.

Referring to Figures 1 to 4, a first embodiment of the present invention for storing a biological tissue for a long period of time, the storage device 1 comprising a circular container 10 and a covering body 20; the circular container 10 is composed of biological tissue The container 11 is formed by injecting fresh blood 12, which is made of a hard material into a circular sphere having a diameter of 0.8 cm and a hollow chamber 111 having a diameter of 0.4 cm, and an outer circumference of a small cone. The inlet 112 is filled with fresh blood 12, and the conical injection port 112 is filled with a spherical or conical filler 113, and the conical injection port 112 is sealed by the glue 114 to be sealed. The circular container 10 is composed of a liquid turpentine 21 which is coated on the outer periphery of the circular container 10, and the liquid rosin 21 is solidified to form a solid turpentine coating body 20 for long-term storage. The covering body 20 can be stored in the underground natural soil 50 if necessary (see Fig. 9), and after a few years of storage, it is gradually converted into amber.

The first embodiment is made as follows:

A. The biological tissue container 11 having a circular spherical body having a diameter of 0.8 cm is made of a material of a rigid container, and a hollow chamber 111 having a diameter of 0.4 cm is provided inside, and a tapered injection port 112 having a large outer diameter is provided on the outer circumference. It should be noted that the size and shape of the container listed in this manual are for reference only, and the size and shape of the container can be changed according to actual needs.

B. Referring to FIG. 3, the fresh blood 12 is taken out by the syringe 30, and the fresh blood 12 is injected into the hollow chamber 111 from the conical injection port 112 in the sealed nitrogen environment, and is discharged from the conical injection port 112. After all the air in the chamber 111 is filled with fresh blood 12, the cone injection port 112 is filled with a spherical or conical filler 113, and the conical injection port 112 is sealed with the glue 114. Make it a circular container 10.

C. Referring to Fig. 4, the initially condensed (semi-liquid) turpentine 21 is placed on the bottom of the lower cover 41 of the 1.2 cm diameter circular mold 40, and is pressed at the correct position using a mold (not shown). After the tank, the circular container 10 containing the blood 12 is placed, and after the upper cover 42 of the circular mold 40 is covered, the liquid turpentine 21 is poured into the upper cover opening 43 to allow the circular container 10 to be accommodated therein. Preset groove position.

D. After the liquid rosin 21 is filled and filled, the rosin 21 is gradually hardened to constitute the covering body 20.

Among them, it is filled in a nitrogen environment to prevent oxygen from penetrating into the sphere, causing bacteria to breed and cause blood deterioration.

Please refer to the fifth embodiment of the present invention, which is a second embodiment of the method for the long-term preservation of biological blood and the device thereof. The structure of the living organism is the same as that of the first embodiment and has the same function. The difference between the two is that the storage device 1A includes a circular container 10A and a covering body 20; the circular container 10A is formed by injecting fresh blood 12 into the biological tissue container 11A, and the biological tissue container 11A is made of a soft material. Or the rubber is made into a hollow chamber 111A having a diameter of 0.8 cm and a diameter of 0.4 cm. The outer circumference of the hollow chamber 111A is not opened, and the needle 31 of the syringe 30 is directly inserted into the hollow chamber 111A to inject fresh blood 12, and the syringe is taken out. After the needle 31 of 30, it is naturally contracted by a soft material, and it is directly adhered to form a closed circular container 10A.

The second embodiment is made as follows:

A. A 0.8 cm diameter spherical biological tissue container 11A was made of a soft material or rubber, and the inside was a hollow chamber 111A having a diameter of 0.4 cm.

B. The fresh blood 12 is taken out by the syringe 30, and the fresh blood 12 is injected into the hollow chamber 111A by the needle 31 of the syringe 30 in the nitrogen atmosphere, and the other empty needle 32 is inserted into the hollow chamber 111A. After the fresh air 12 is filled in the hollow chamber 111A, the needle 31 and the empty needle 32 of the syringe 30 are taken out and sealed directly to form a circular container 10A.

C. After the initially condensed (semi-liquid) turpentine 21 is placed on the bottom of the lower cover 41 of the circular die 40 of a diameter of 1.2 cm, the groove is pressed at the correct position using a mold (not shown), and then placed. The circular container 10A having the blood 12, because the semi-liquid rosin 21 has a certain adhesiveness, fixes the ball to the predetermined groove position, covers the upper cover 42 of the circular mold 40, and then passes the upper cover. The opening 43 is filled with liquid turpentine 21.

D. The turpentine 21 is hardened to a solid to constitute the covering body 20.

Referring to Fig. 9, the creation holding device 1, 1A is buried in the soil 50, and the coating body 20 obtained by hardening the turpentine 21 is gradually transformed into amber after a plurality of years to form amber.

Referring to Figures 10 and 11, the creation holding device 1, 1A can also be machined into various shapes and machined into a necklace 60 or a ring to be processed into a ring 70.

The biological tissue of the present invention is exemplified by human blood, and may of course be replaced with the bone marrow, brain juice, stem cells, cells, hair, fish, shrimp blood or insect juice or plant juice of the animal to obtain the same effect.

The above-mentioned biological tissue containers 11, 11A are described by taking a spherical body as an example, and of course, an ellipsoid or an annular body or a hollow annular body or other suitable shapes may be substituted to obtain the same effect.

It should be specifically noted that the above-mentioned biological tissue container 10, 10A, 11, 11A or the covering body 20 coated on the outside of the container 10, 10A, 11, 11A of the present invention should include all natural materials. The resin produced by the pine plant can be extended to use the following materials: (1) Resin, which refers to the fat liquid produced by all natural trees; (2) generally acts through the esterification process, and then organic a synthetic resin treated with a compound; and; (3) a rubber containing silicone rubber of various hardnesses and transparency.

In summary, the creation is indeed a rather excellent idea, and the application for the creation of a patent is made according to law; however, the content of the above description is only a description of the preferred embodiment of the creation, and is extended by the technical means of the creation. Changes are subject to the scope of the patent application of this creation, which is hereby stated.

1, 1A‧‧‧ save device
10, 10A‧‧‧ round container
11, 11A‧‧‧ biological tissue containers
111, 111A‧‧‧ hollow room
112‧‧‧ Tapered inlet
113‧‧‧Filling
114‧‧‧Compound
12‧‧‧ blood
20‧‧ ‧ wrap
21‧‧‧ rosin
30‧‧‧Syringe
31‧‧‧ needle
32‧‧‧ Empty needle
40‧‧‧Circular mould
41‧‧‧Under the cover
42‧‧‧上盖
43‧‧‧上盖洞口
50‧‧‧ soil
60‧‧‧Necklace
70‧‧‧ Ring

1 is a schematic cross-sectional view of a storage device according to a first embodiment of the present invention; FIG. 2 is a schematic cross-sectional view of a biological tissue container according to a first embodiment of the present invention; FIG. 5 is a schematic cross-sectional view of a storage device according to a second embodiment of the present invention; FIG. 6 is a cross-sectional view of a storage device according to a second embodiment of the present invention; The figure is a schematic view of the filling blood section of the second embodiment of the creation; FIG. 8 is a schematic cross-sectional view of the covering body of the second embodiment of the creation; FIG. 9 is a schematic cross-sectional view of the buried soil in the creation example; A perspective view of an application example of the present invention; FIG. 11 is a perspective view of another example of the authoring application.

1‧‧‧Save device

10‧‧‧round container

11‧‧‧ Biological tissue containers

111‧‧‧ hollow room

112‧‧‧ Tapered inlet

113‧‧‧Filling

114‧‧‧Compound

12‧‧‧ blood

20‧‧ ‧ wrap

21‧‧‧ rosin

Claims (10)

A biological tissue long-term preservation method comprises the following steps: A. A biological tissue container (11) made of a hard material and having a circular sphere, and a hollow chamber (111) is arranged inside, and a small outer small cone is opened on the outer circumference. Shape injection port (112); B, in the nitrogen environment space, fresh blood (12) is injected into the hollow chamber (111) from the conical injection port (112), and the hollow chamber (111) is filled with fresh blood. After (12), all the air in the hollow chamber (111) is completely discharged, and the tapered injection port (112) is filled with the filler (113), and the cone is injected with the glue (114). The inlet (112) is sealed to form a sealed circular container (10); C. The initially set turpentine (21) is placed at the bottom of the circular mold (40) and placed in a round container containing blood (10) ), refilling the liquid turpentine (21) to keep the circular container (10) therein; D. After the rosin is hardened into a solid, the coating body (20) is formed for long-term storage. The invention relates to a method for long-term preservation of biological tissues, which comprises the following steps: A. a biological tissue container (11A) made of a soft sphere or rubber, with a hollow chamber (111A) inside; B, with a syringe (30) Fresh blood (12) is taken, and fresh blood (12) is directly inserted into the hollow chamber (111A) with a needle (31) of the syringe (30) in a nitrogen atmosphere, and another empty needle (32) is inserted into the hollow. All the air in the chamber (111A), after filling the hollow chamber (111A) with fresh blood (12), withdraw the needle (31) of the syringe (30) and the empty needle (32) to directly seal themselves. a circular container (10A) to be sealed; C. Place the initially set turpentine (21) on the bottom of the circular mold (40) and place a circular container (10A) containing fresh blood (12). Refilling the liquid turpentine (21) to keep the circular container therein; D. After the rosin (21) is hardened into a solid, the coating body (20) is formed for long-term storage. A biological tissue long-term preservation device comprising: a circular container (10, 10A) formed by injecting fresh blood (12) into a biological tissue container (11, 11A) provided with a hollow chamber (111, 111A); The covering body (20) is wrapped around the outer circumference of the circular container (10, 10A); the circular container (10, 10A) is covered by the covering body (20) to make the circular container ( The fresh blood (12) in 10, 10A) is completely isolated from air and water and becomes a good long-term storage device. The biological tissue long-term preservation device according to claim 3, wherein the biological tissue container (11) is made of a hard material and has a hollow spherical body having a hollow chamber (111), and a tapered portion is formed on the outer circumference thereof. Injection inlet (112), after injecting fresh blood (12) from the conical injection port (12), sealing the conical injection port (112) to form a circular container (10); the biological tissue container (11) A hollow sphere having a hollow chamber (111A) made of a soft material or rubber, and after the fresh blood (12) is injected into the syringe (30), the needle of the syringe (30) is removed, and the soft material is self-contained. The sealing function is restored to form a sealed circular container (10A). The biological tissue long-term preservation device according to claim 3, wherein the coating body (20) is formed by solidifying liquid rosin (21) into a solid and converting to form amber after several years. The method and apparatus for long-term preservation of biological tissues according to claim 1, wherein the biological tissue container (11, 11A) is a sphere, an ellipsoid, a ring, and other suitable polygonal spheres. The method and apparatus for long-term preservation of biological tissues according to claim 1, wherein the biological tissue comprises blood, bone marrow, brain juice, stem cells, cells, hair, body fluids, fish, shrimp blood, insects. Juice, plant juice. The method and apparatus for long-term preservation of biological tissues according to claim 1 or 2 or 3, wherein the rosin further comprises a natural resin of a tree fat liquid produced by all natural pine plants. The method and apparatus for long-term preservation of biological tissues according to claim 1 or 2 or 3, wherein the rosin further comprises all artificial chemical resins treated through an esterification step. The method and apparatus for long-term preservation of biological tissues according to claim 1 or 2 or 3, wherein the turpentine further comprises all of the various hardnesses and transparency of the keloid or rubber.