TW201723483A - Machine and method for automatically processing samples - Google Patents

Abstract

A machine for automatically process samples includes a rack, a rail module, a holding device, a sucking and injecting device, a centrifuge device, and a central processor. The rail module is movable disposed on the rack. The holding device has a plurality of holding troughs located in a moving range of the rail module. The sucking and injecting device is fixed to the rail module and configured to suck and inject a liquid to be processed in the holding troughs. The centrifuge device has a motor and a runner disc driven to rotate by the motor. The central processor is configured to control the rail module to move and thus reciprocally move the sucking and injecting device to the holding device and the runner disc. The central processor is further configured to control the motor to drive the runner disc and control the sucking and injecting device to inject the liquid to be processed into the runner disc during the rotation of the runner disc.

Description

Machine for automatically processing samples and method for automatically processing samples

The present invention relates to a machine for automatically processing samples and a method for automatically processing samples.

The preparation and centrifugation of samples and reagents are two common steps in biomedical experiments or experiments. Generally in the laboratory or laboratory, the experiment or test operator must manually mix the sample and reagents, then pour into the centrifuge tube and centrifuge in a centrifuge to stratify the substances with different densities by centrifugal force. Obtain the target substance or perform subsequent experiments.

However, the two important steps mentioned above often have to be operated alternately multiple times in a complicated experiment or test process, occupying a large amount of manpower and time, and also prone to human error or error, thereby affecting the correctness of the experiment or experiment. Repeatability.

Therefore, how to propose a machine and method for processing samples that can solve the above problems is one of the problems that the industry is eager to invest in research and development resources.

In view of the above, it is an object of the present invention to provide a machine and method for automatically processing samples that reduce the amount of labor and time required in the process of experimentation or testing.

In order to achieve the above object, according to an embodiment of the present invention, a machine for automatically processing a sample includes a frame body, a slide rail module, a placement device, a suction device, a centrifugal device, and a central processing machine. The slide rail module is movably disposed on the frame body. The placement device has a plurality of placement slots. The placement slot is disposed within the movement range of the slide rail module. The suction device is fixed to the slide rail module for sucking and sucking the liquid in the placement tank. The centrifugal device has a motor and a runner disk that is driven by the motor to rotate. The central processing unit is arranged to control the movement of the slide rail module, thereby driving the suction device to reciprocately move to the placement device and the flow path disc. The central processing unit is also arranged to control the motor to drive the flow path disc and to control the suction means to drip liquid into the flow path disc during the rotation of the flow path disc.

In one or more embodiments of the present invention, the moving slide module includes three axis moving slide modules for respectively moving in three directions substantially perpendicular to each other.

In one or more embodiments of the invention, the suction device comprises at least one syringe. When the suction device moves to the flow path disc, the syringe injector is aligned with at least one injection port of the flow path disc.

In one or more embodiments of the present invention, the flow channel disc further includes a collection box and a flow channel structure, and the flow path structure includes a first flow path and a second flow path that communicate with the collection box.

In order to achieve the above object, according to another embodiment of the present invention, a method for automatically processing a sample includes: (a) providing a pretreatment sample in a placement device; (b) driving a flow path disc rotation; (c) controlling suction The spitting device moves to the placement device to draw the pretreatment sample; (d) moves the suction device to the flow channel disc; and (e) controls the suction device to drip the pretreatment sample into the flow channel during the rotation of the flow channel disc The first injection port of the disc.

In one or more embodiments of the present invention, the control suction device has a dropping rate of the pretreatment sample dropped into the first injection port of substantially 0.5 ml/min to 40 ml/min.

In one or more embodiments of the present invention, the rotational speed of the drive flow path disc is substantially 1000 rpm to 3000 rpm.

In one or more embodiments of the present invention, the above step (e) separates the analyte of the pretreated sample from the first flow path of the flow channel disc to the collection box.

In one or more embodiments of the present invention, the above step (a) includes controlling the suction device to mix the original sample with the first reagent in the placement device to generate a pretreatment sample. The original sample is a blood sample and the first reagent contains a buffer and a red and white blood cell binding reagent.

In one or more embodiments of the present invention, the method for automatically processing a sample further includes: (f) controlling the suction device to apply the second reagent or the rotation device during the rotation of the flow channel disc before and after the step (e) / Drop the third reagent into the runner disc.

In one or more embodiments of the present invention, the second reagent is a density gradient liquid. The third reagent is a buffer. The second reagent and the third reagent are simultaneously dropped into the first injection port and the second injection port of the flow path disc, respectively.

In one or more embodiments of the present invention, the method for automatically processing a sample further comprises: (g1) controlling the suction device to drop the fourth reagent into the flow channel disc; (g2) driving the flow channel disc to stop Rotating; and (g3) driving the flow path disc to rotate after the flow path disc stops rotating for the first predetermined time.

In one or more embodiments of the present invention, the fourth reagent comprises a cell membrane stain and a cell fixative.

In one or more embodiments of the present invention, the method for automatically processing a sample further comprises: (h1) controlling the suction device to drop the fifth reagent into the flow channel disc; (h2) driving the flow path disc to stop Rotating; and (h3) driving the runner disc to rotate after the runner disc stops rotating for a second predetermined time.

In one or more embodiments of the present invention, the fifth reagent comprises a nuclear staining agent and a cell membrane permeabilizing agent.

In summary, the automatic processing sample machine of the present invention and the method for automatically processing the sample can combine and automate the mixing and centrifugation of the sample and the reagents - the common steps of the two biomedical experiments or tests. Especially for some difficult and complicated experiments or experiments, it is necessary to continuously mix reagents and drip samples or/and reagents during centrifugation, which is an operation behavior that is difficult to achieve by hand. The artificial machine can overcome the above-mentioned artificial labor. The problem is to reduce the amount of manpower and time required to conduct an experiment or test, and to reduce human error or error, thereby improving the accuracy and repeatability of the experiment or test.

The above description is only for explaining the problems to be solved by the present invention, the technical means for solving the problems, the effects thereof, and the like, and the specific details of the present invention will be described in detail in the following embodiments and related drawings.

The embodiments of the present invention are disclosed in the following drawings, and the details of However, it should be understood that these practical details are not intended to limit the invention. That is, in some embodiments of the invention, these practical details are not necessary. In addition, some of the conventional structures and elements are shown in the drawings in a simplified schematic manner in order to simplify the drawings.

Please refer to FIG. 1 , which is a perspective view of a machine 100 for automatically processing samples according to an embodiment of the present invention. As shown in FIG. 1, in the present embodiment, the machine table 100 for automatically processing samples includes a frame body 110, a slide rail module 120, a placement device 130, an absorption device 140, a centrifugal device 150, and a central processing unit 160. The slide rail module 120 is movably disposed on the frame body 110. The placement device 130 has a plurality of placement slots 131. The placement slot 131 is disposed within the range of movement of the slide rail module 120. The suction device 140 is fixed to the slide rail module 120 for sucking and sucking various liquids in the placement groove 131. The centrifugal device 150 has a motor 151 and a flow path disk 152 that is driven to rotate by the motor 151. The central processing unit 160 is coupled to the slide rail module 120, the suction and discharge device 140, and the motor 151, and can be disposed on the frame body 110 or outside the frame body 110, and the position is not limited. The central processing unit 160 is arranged to control the movement of the slide rail module 120, thereby driving the suction and discharge device 140 to reciprocately move to the placement device 130 and the flow path disc 152 (shown in phantom in FIG. 1). The central processing unit 160 is also arranged to control the motor 151 to drive the flow path disc 152 and control the suction unit 140 to drip liquid into the flow path disc 152 during the rotation of the flow path disc 152.

In some embodiments of the present invention, the slide rail module 120 is a three-axis moving slide rail module. For example, as shown in FIG. 1 , the slide rail module 120 includes an X-axis moving slide rail 121 , a Y-axis moving slide rail 122 , and a Z-axis moving slide rail 123 for respectively respectively along three substantially perpendicular to each other. The direction moves relatively. The X-axis moving rail 121 is disposed on the frame body 110 and movable relative to the frame body 110 in the X-axis direction. The Y-axis moving rail 122 is disposed on the frame body 110 and movable relative to the frame body 110 in the Y-axis direction. The placement device 130 is disposed on the Y-axis moving rail 122, and is further movable by the Y-axis moving rail 122 to move in the Y-axis direction. The Z-axis moving rail 123 is disposed on the X-axis moving rail 121 and is movable relative to the frame body 110 in the Z-axis direction. The suction device 140 is fixed to the Z-axis moving rail 123. Therefore, by controlling the X-axis moving rail 121 to be actuated by the central processing unit 160, the suction device 140 can be driven to reciprocate to the placement device 130 and the flow path disc 152; and the central processing unit 160 controls the Y-axis moving rail. 122, the device 130 can be driven to align the specific placement slot 131 and the suction device 140 in the Z-axis direction; when the central processing unit 160 controls the Z-axis movement rail 123 to act, the suction can be performed. When the device 140 moves above the placement device 130, the suction device 140 is moved downward to the specific placement slot 131 for suction or injection, or when the suction device 140 moves over the flow channel disc 152. The suction device 140 moves downward to the injection port for injection. In some embodiments of the present invention, the slide rail module 120 further includes a stepping motor controller 124 for controlling the actuation of the X-axis moving rail 121, the Y-axis moving rail 122, and the Z-axis moving rail 123.

In some embodiments of the present invention, the central processing unit 160 is a computer, a single-chip processor, or a programmable logic controller (PLC), but the invention is not limited thereto.

In some embodiments of the present invention, the suction device 140 includes at least one syringe, but the invention is not limited thereto. In an embodiment of the invention, the suction device 140 includes a syringe syringe 140a and a syringe syringe 140b, as shown in FIG.

2 is a perspective view showing a flow path disk 152 of the centrifugal device 150 according to an embodiment of the present invention. As shown in Fig. 2, in the present embodiment, the flow path disk 152 includes a flow path structure 152a and a collecting case 152b. The flow channel structure 152a of the present invention comprises at least one injection port and one outlet. In an embodiment of the invention, the flow channel structure 152a includes a first injection port 152a1, a first flow channel 152a4 (shown by a broken line in FIG. 2), The second injection port 152a2, the second flow path 152a5 (indicated by a broken line in Fig. 2), and the outlet 152a3. The first injection port 152a1 and the second injection port 152a2 are both connected to the outlet 152a3. The first injection port 152a1 is communicated to the outlet 152a3 via the first flow path 152a4, and the second injection port 152a2 is communicated to the outlet 152a3 via the second flow path 152a5. It should be noted that the first flow channel 152a4 and the second flow channel 152a5 of the present invention may have the same length or different lengths, and the number and shape of the flow channels are not limited thereby. In addition, the flow path disk of the present invention does not require a complicated design such as a valve. The collection box 152b is disposed at the outlet 152a3 of the flow path structure 152a. During the rotation of the flow path disc 152, the suction processing device 140 can be controlled by the central processing unit 160 to drop the pretreatment sample into the first injection port 152a1. Under the action of the centrifugal force, the analyte of the pretreatment sample is separated and flows from the first injection port 152a1 to the outlet 152a3 via the first flow path 152a4, and is collected in the collection box 152b. In addition, during the rotation of the flow path disk 152, the suction device 140 can be controlled to drop a reagent into the second injection port 152a2 through the central processing unit 160. Similarly, under the action of the centrifugal force, some components of the reagent are separated and flow from the second injection port 152a2 to the outlet 152a3 via the second flow path 152a5, and are collected in the collection box 152b and analyzed. The substances are mixed to produce a chemical reaction.

In some embodiments of the present invention, the actual operation process of the machine 100 for automatically processing the sample may be as follows: (1) placing the original sample and reagent required for the experiment or test in the placement device 130 before the experiment or the test, respectively. (2) According to the guiding flow of the central processing unit 160 (for example, through a screen), the processing steps and reagents of the experiment or test such as the placement device 130, the suction device 140, and the centrifugal device 150 are set. The amount, the rotation speed of the centrifuge device, the time, etc.; and (3) the use of the slide module 120 and the suction device 140 through the central processing unit 160, according to the steps and methods of the experimental or experimental planning, the original sample and the reagent in the placement device The 130 is moved to make a preparation, or the prepared pre-treated sample and reagent are moved to the centrifuge device 150 for centrifugation.

Please refer to FIG. 3, which is a flow chart showing a method for automatically processing samples according to an embodiment of the present invention. As shown in Fig. 3, in conjunction with Fig. 1 and Fig. 2, the present embodiment is an example of separating circulating cancer cells (CTCs) in the blood of cancer patients, and the test procedure requires a sample and The reagent is formulated and centrifuged.

It should be noted that cancer metastasis is the leading cause of cancer-related death. The spread of cancer cells through the blood circulation is an important intermediate step, which also reflects the switch of regional diseases to systemic diseases. During the evolution of the disease, circulating cancer cells provide an opportunity to assess the biological characteristics of the cancer, allowing physicians to respond quickly to treat patients with the most appropriate specific targeted therapy and possibly prevent metastasis in the near future. Sexual disease.

For example, the method of automatically processing a sample of the present invention includes steps S101 to S113.

Before performing step S101, various liquids required for the test, such as the original sample (such as cancer patient blood), various reagents such as buffer, red blood cell binding reagent, density gradient liquid, immunofluorescent dye, cell membrane table, may be used. The dyeing agent, the cell fixing solution, the cell membrane permeabilizing agent, and the like are placed in the corresponding placement grooves of the placement device 130 (such as the placement grooves 131a, 131b shown in Fig. 1).

Next, step S101 can be performed to complete the step of generating the pre-processed samples.

In step S101, the suction device 140 is controlled to dispense the original sample and the first reagent in the placement device 130 to generate a pre-processed sample. Wherein, the first reagent comprises a buffer and a red blood cell binding reagent. In other embodiments of the present invention, the pre-processed sample may be manually prepared and placed in the placement device 130.

For example, in the experiment of separating circulating cancer cells in the blood of cancer patients, before step S101, a buffer may be added to the placement tank 131a, so that the suction device 140 can absorb the different reagents and can be used in the cleaning tank. The reagent remaining in the aspirating device 140 is removed to prevent contamination of the sample or individual reagents. In the present embodiment, the original sample is already present in the placement groove 131b. Specifically, in step S101, the buffer solution and the red blood cell binding reagent can be separately added to the placement groove 131b by the syringe injector 140a and the syringe syringe 140b. The diluted blood is obtained, and the diluted blood is sucked up using a syringe syringe of one of the suction devices 140 and then the diluted blood is spit out to uniformly mix them to generate a pretreated sample. In some embodiments of the invention, the blood dilution and mixing step dispenses the pretreated sample at a rate of one injection per minute and continues for twenty minutes.

Then, steps S102 to S105 may be sequentially performed to complete the steps of pre-processing sample centrifugation and collecting the analyte.

In step S102, the flow path disc 152 is driven to rotate. In some embodiments of the present invention, the rotational speed of the driving flow path disc 152 may be 1000 rpm to 3000 rpm, preferably 2500 rpm, but the invention is not limited thereto.

In step S103, the suction device 140 is controlled to move to the placement device 130 to take up the pre-processed sample. In the present embodiment, the suction device 140 and the placement slot 131b are controlled to move to a relative position, and the syringe injector (such as the syringe injector 140a) of the suction device 140 is controlled to take the pretreatment sample.

In step S104, the suction device 140 is moved to the flow path disc 152. In the present embodiment, the syringe injector 140a can be controlled to be aligned with the first injection port 152a1 of the flow path disk 152.

In step S105, during the rotation of the flow path disc 152, the suction and discharge device 140 is controlled to drop the pretreatment sample into the flow path disc 152, such as dropping into the first injection port 152a1, and the dropping speed may be substantially 0.5. Ml/min to 40 ml/min, preferably 20 ml/min. Wherein, by the execution of step S105, the pre-processed sample can be transferred from the first injection port 152a1 through the flow channel having a predetermined length (such as the first flow channel 152a4 in this embodiment) by centrifugal force, so that the analyte is from the pre-processed sample. The collection box 152b is separated into the flow path disc 152.

In some embodiments of the invention, step S106 may be further included in the step of blood centrifugation (eg, prior to step S103).

In step S106, the suction device 140 is controlled to drop the second reagent or/and the third reagent into the flow path disk 152. Wherein, the second reagent can be a density gradient liquid. The third reagent can be a buffer. In an embodiment of the present invention, step S106 may include first controlling the suction device 140 to move to the placement device 130, controlling the syringe injector 140a to absorb the density gradient liquid, and controlling the syringe injector 140b to absorb the buffer, and then the suction device. 140 moves to the upper portion of the flow path disc 152, so that the syringe injector 140a and the syringe injector 140b are respectively aligned with the first injection port 152a1 and the second injection port 152a2, so that the density gradient liquid and the buffer solution are dropped into the flow channel simultaneously or sequentially. Disc 152. Alternatively, the density gradient can be dropped from the same injection port as the pretreated sample to facilitate mixing during centrifugation. In the present embodiment, the density gradient liquid can enter the collection box 152b from the first injection port 152a1 through the first flow path 152a4, and the buffer can enter the collection box 152b from the second injection port 152a2 through the second flow path 152a5.

Next, after a predetermined amount of the second reagent and the third reagent has been dropped into the flow path disk 152, the subsequent step S103 is performed.

In some embodiments of the invention, step S107 may be further included in the step of blood centrifugation (eg, after step S105). In step S107, the suction/discharging device 140 is controlled to drop the second reagent or/and the third reagent into the flow channel disc 152. Step S107 is the same as step S106, and will not be described again.

In some embodiments of the present invention, the foregoing steps S105 and S107 may be performed four times, but the invention is not limited thereto. It is worth noting that from step S102 to step S105, the flow path disc 152 is rotated.

Next, steps S108 to S114 can be performed to complete the step of cell staining.

In step S108, the suction device 140 is controlled to drop the fourth reagent into the flow path disk 152. Wherein, the fourth reagent comprises a cell membrane epiderm and a cell fixative. In some embodiments of the invention, the step of mixing the cell membrane epidermal agent with the cell fixative reagent may also be included in the method of the present invention for automatically processing a sample. Additionally, in some embodiments of the invention, the fourth reagent may be dropped into the first injection port 152a1 or the second injection port 152a2 by the syringe injector 140b at a rate similar to that of step S105.

In step S109, the drive flow path disc 152 is stopped from rotating. In other words, from step S102 to step S108, the flow path disc 152 is in a rotated state.

In step S110, after the flow path disc 152 stops rotating for the first predetermined time, the flow path disc 152 is driven to rotate. In some embodiments of the invention, the first predetermined time is about 30 minutes to achieve an effective dyeing effect, but the invention is not limited thereto.

In step S111, the suction device 140 is controlled to drop the fifth reagent into the flow path disk 152. The fifth reagent comprises a nuclear staining agent and a cell membrane permeabilizing agent. In some embodiments of the invention, the reagent mixing step of the nuclear staining agent and the cell membrane permeabilizing agent may also be included in the method of automatically processing the sample of the present invention. Further, in some embodiments of the present invention, the fifth reagent may be dropped into the first injection port 152a1 or the second injection port 152a2 by the syringe injector 140b, and the dropping speed may be as fast as the step S105.

In step S112, the drive flow path disc 152 is stopped from rotating.

In step S113, after the flow path disc 152 stops rotating for a second predetermined time, the flow path disc 152 is driven to rotate. In some embodiments of the invention, the second predetermined time is about 40 minutes to achieve an effective dyeing effect, but the invention is not limited thereto.

In step S114, the drive flow path disc 152 is stopped from rotating.

Finally, the experimental or post-test processing steps such as taking out the placement device 130, the suction device 140, and the centrifuge device 150 can be sequentially performed according to the guided flow of the central processing unit 160 (for example, through a screen), thereby completing the present invention. A method of automatically processing samples.

From the above detailed description of the specific embodiments of the present invention, it can be clearly seen that the automatic processing sample machine of the present invention and the method for automatically processing the sample can mix and centrifuge the sample and the reagent - the two biochemicals Common steps in a class of experiments or tests, combined and automated. Especially for some difficult and complicated experiments or experiments, it is necessary to continuously mix reagents and drip samples or/and reagents during centrifugation, which is an operation behavior that is difficult to achieve by hand. The artificial machine can overcome the above-mentioned artificial labor. The problem is to reduce the amount of manpower and time required to conduct an experiment or test, and to reduce human error or error, thereby improving the accuracy and repeatability of the experiment or test.

The present invention has been disclosed in the above embodiments, and is not intended to limit the scope of the present invention, and the invention may be modified and modified in various ways without departing from the spirit and scope of the invention. The scope is subject to the definition of the scope of the patent application.

100‧‧‧Automatically processed sample machine
110‧‧‧ ‧ frame
120‧‧‧Slide module
121‧‧‧X-axis moving rails
122‧‧‧Y-axis moving rail
123‧‧‧Z-axis moving rail
124‧‧‧Stepper motor controller
130‧‧‧Placement device
131a, 131b‧‧‧ placement slots
140‧‧‧Sucking device
140a, 140b‧‧‧ syringe syringe
150‧‧‧ centrifugal device
151‧‧‧Motor
152‧‧‧Flower discs
152a‧‧‧Flow structure
152a1‧‧‧ first injection port
152a2‧‧‧second injection port
152a3‧‧‧Export
152a4‧‧‧First runner
152a5‧‧‧Second runner
152b‧‧‧ collection box
160‧‧‧Central Processing Machine
S101～S114‧‧‧Steps

The above and other objects, features, advantages and embodiments of the present invention will become more <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; . Fig. 2 is a perspective view showing a flow path disk of the centrifugal device according to the embodiment of the present invention. FIG. 3 is a flow chart showing a method of automatically processing a sample according to an embodiment of the present invention.

100‧‧‧Automatically processed sample machine

110‧‧‧ ‧ frame

120‧‧‧Slide module

121‧‧‧X-axis moving rails

122‧‧‧Y-axis moving rail

123‧‧‧Z-axis moving rail

124‧‧‧Stepper motor controller

130‧‧‧Placement device

131a, 131b‧‧‧ placement slots

140‧‧‧Sucking device

140a, 140b‧‧‧ syringe syringe

150‧‧‧ centrifugal device

151‧‧‧Motor

152‧‧‧Flower discs

160‧‧‧Central Processing Machine

Claims (16)

A machine for automatically processing a sample, comprising: a frame body; a slide rail module movably disposed on the frame body; a placement device having a plurality of placement slots disposed in the movement range of the slide rail module a suction device fixed to the slide rail module for sucking and sucking one of the liquids in the placement slots; a centrifugal device having a motor and a flow path disc driven by the motor; And a central processing unit configured to control movement of the slide rail module to drive the suction and discharge device to reciprocate to the placement device and the flow path disc, wherein the central processing unit is further configured to control the motor to drive the flow The disc is controlled, and the suction device is controlled to drip the liquid into the flow path disc during the rotation of the flow path disc. The machine for automatically processing samples according to claim 1, wherein the slide module comprises three axis moving slides for respectively moving in three directions substantially perpendicular to each other. The apparatus for automatically processing a sample according to the item 2, wherein the placing device and the suction device are respectively fixed to different axis moving rails. The apparatus for automatically processing a sample according to claim 1, wherein the suction device comprises at least one syringe syringe, and when the suction device moves to the flow channel disc, the at least one syringe is aligned At least one of the flow path discs is injected into the mouth. The apparatus for automatically processing a sample according to claim 1, wherein the flow path disc further comprises a collection box and a first-class track structure, and the flow path structure comprises a first flow path and a first one connected to the collection box. Second runner. A method for automatically processing a sample, comprising: (a) providing a pretreatment sample in a placement device; (b) driving a first-class disk rotation; (c) controlling the suction device to move to the placement device to absorb the sample Pre-treating the sample; (d) moving the suction device to the flow path disc; and (e) controlling the suction device to drop the pre-treatment sample into the flow path disc during the rotation of the flow path disc One of the first injection ports. The method of automatically processing a sample according to claim 6, wherein controlling the suction device to drop the pretreatment sample into the first injection port has a dropping speed of substantially 0.5 ml/min to 40 ml/min. The method of automatically processing a sample according to claim 6, wherein the one of the rotations of the flow path disc driving is substantially 1000 rpm to 3000 rpm. The method of automatically processing a sample according to claim 6, wherein the step (e) is: separating one of the pretreatment samples from the first flow path of the flow path disc to a collection box. The method of automatically processing a sample according to claim 6, wherein the step (a) comprises: controlling the suction device to mix an original sample and a first reagent in the placement device to generate the pretreatment sample, the original The sample is a blood sample, and the first reagent comprises a buffer and a red blood cell binding reagent. The method for automatically processing a sample according to Item 6 of the claim further comprises: (f) controlling the suction device to apply a second reagent or / during the rotation of the flow channel disc before and after the step (e); And a third reagent is dropped into the flow path disc. The method of automatically processing a sample according to claim 11, wherein the second reagent is a density gradient liquid, the third reagent is a buffer, and the second reagent and the third reagent are simultaneously dropped into the sample. The first injection port and the second injection port of one of the flow path disks. The method for automatically processing a sample according to claim 6, further comprising: (g1) controlling the suction device to drop a fourth reagent into the flow path disc; (g2) driving the flow path disc to stop Rotating; and (g3) driving the flow path disc to rotate after the flow path disc stops rotating for a first predetermined time. The method of automatically processing a sample according to claim 13, wherein the fourth reagent comprises a cell membrane epidermal agent and a cell fixative. The method for automatically processing a sample according to claim 13 , further comprising: (h1) controlling the suction device to drop a fifth reagent into the flow channel disc; (h2) driving the flow path disc to stop Rotating; and (h3) driving the flow path disc to rotate after the flow path disc stops rotating for a second predetermined time. The method of automatically processing a sample according to claim 15, wherein the fifth reagent comprises a nuclear staining agent and a cell membrane permeabilizing agent.