TW201612523A - Method for evaluating neural function by establishing analysis module - Google Patents
Method for evaluating neural function by establishing analysis moduleInfo
- Publication number
- TW201612523A TW201612523A TW103133108A TW103133108A TW201612523A TW 201612523 A TW201612523 A TW 201612523A TW 103133108 A TW103133108 A TW 103133108A TW 103133108 A TW103133108 A TW 103133108A TW 201612523 A TW201612523 A TW 201612523A
- Authority
- TW
- Taiwan
- Prior art keywords
- nerve cells
- fluorescence
- neural function
- analysis module
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000001537 neural effect Effects 0.000 title abstract 3
- 238000004458 analytical method Methods 0.000 title abstract 2
- 210000002569 neuron Anatomy 0.000 abstract 8
- 210000003050 axon Anatomy 0.000 abstract 4
- 210000001787 dendrite Anatomy 0.000 abstract 4
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 238000010191 image analysis Methods 0.000 abstract 1
- 238000003384 imaging method Methods 0.000 abstract 1
- 230000000946 synaptic effect Effects 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a method for evaluating neural function by establishing an analysis module. The method comprises steps of using a fluorescent microscope imaging system to capture a cultured cell which comprises nerve cells and non-neuronal cells and is labeled with a plurality of fluorescence to conduct an image analysis; choosing the nerve cells labeled with a plurality of axon and dendrite fluorescence according to nucleus area and fluorescence intensity to exclude non-neuronal cells; calculating size of nerve cell body, length and branch number of axons and dendrites to confirm growth of the axons and the dendrites of the nerve cells; and calculating number of synaptic puncta of the nerve cells according to scope of the plurality of axon and dendrite fluorescence to confirm growth of the nerve cells, so as to evaluate neural function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW103133108A TWI548873B (en) | 2014-09-24 | 2014-09-24 | Method for quick evaluating neural function by establishing analysis module |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW103133108A TWI548873B (en) | 2014-09-24 | 2014-09-24 | Method for quick evaluating neural function by establishing analysis module |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201612523A true TW201612523A (en) | 2016-04-01 |
| TWI548873B TWI548873B (en) | 2016-09-11 |
Family
ID=56360840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW103133108A TWI548873B (en) | 2014-09-24 | 2014-09-24 | Method for quick evaluating neural function by establishing analysis module |
Country Status (1)
| Country | Link |
|---|---|
| TW (1) | TWI548873B (en) |
-
2014
- 2014-09-24 TW TW103133108A patent/TWI548873B/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| TWI548873B (en) | 2016-09-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Annulment or lapse of patent due to non-payment of fees |