IN2012DN02129A - - Google Patents

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Publication number
IN2012DN02129A
IN2012DN02129A IN2129DEN2012A IN2012DN02129A IN 2012DN02129 A IN2012DN02129 A IN 2012DN02129A IN 2129DEN2012 A IN2129DEN2012 A IN 2129DEN2012A IN 2012DN02129 A IN2012DN02129 A IN 2012DN02129A
Authority
IN
India
Prior art keywords
brightfield
image
generating
biological sample
fluorescent images
Prior art date
Application number
Inventor
Can Ali
j gerdes Michael
O Bello Musodiq
Li Qing
Original Assignee
Gen Electric
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Electric filed Critical Gen Electric
Publication of IN2012DN02129A publication Critical patent/IN2012DN02129A/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration by the use of more than one image, e.g. averaging, subtraction
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20221—Image fusion; Image merging
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

Abstract

A method for generating a brightfield type image, which resembles a brightfield staining protocol of a biological sample, using fluorescent images is provided. The steps comprise acquiring two or more fluorescent images of a fixed area on a biological sample, mapping said fluorescent image into a brightfield color space, and generating a bright field image. Also provided is an image analysis system for generating a brightfield type image of a biological sample using fluorescent images.
IN2129DEN2012 2009-09-29 2010-09-29 IN2012DN02129A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/569,396 US8269827B2 (en) 2009-09-29 2009-09-29 System and methods for mapping fluorescent images into a bright field color space
PCT/SE2010/051046 WO2011040872A1 (en) 2009-09-29 2010-09-29 System and method for generating a brightfield image using fluorescent images

Publications (1)

Publication Number Publication Date
IN2012DN02129A true IN2012DN02129A (en) 2015-08-21

Family

ID=43779911

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2129DEN2012 IN2012DN02129A (en) 2009-09-29 2010-09-29

Country Status (10)

Country Link
US (1) US8269827B2 (en)
EP (1) EP2483668A4 (en)
JP (1) JP5631999B2 (en)
KR (1) KR20120079080A (en)
CN (1) CN102575990B (en)
AU (1) AU2010301166A1 (en)
CA (1) CA2773268A1 (en)
IN (1) IN2012DN02129A (en)
RU (1) RU2012111453A (en)
WO (1) WO2011040872A1 (en)

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US8639013B2 (en) * 2011-08-17 2014-01-28 General Electric Company System and methods for generating a brightfield image using fluorescent images
WO2013142366A1 (en) * 2012-03-19 2013-09-26 Genetic Innovations, Inc. Devices, systems, and methods for virtual staining
CN104520713A (en) * 2012-03-30 2015-04-15 克拉里安特诊断服务公司 Immunoflu0rescence and fluorescent-based nucleic acid analysis on a single sample
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JP6317732B2 (en) 2012-04-30 2018-04-25 ゼネラル・エレクトリック・カンパニイ System and method for performing biomarker quality review scoring and image analysis methods for biological tissue
WO2013181418A2 (en) 2012-05-30 2013-12-05 Clarient Diagnostic Services, Inc. Multiplexed diagnosis method for classical hodgkin lymphoma
US20150226743A1 (en) 2012-10-28 2015-08-13 Clarient Diagnostics Services, Inc. Multiplexed method for diagnosing classical hodgkin lymphoma
WO2015002082A1 (en) * 2013-07-03 2015-01-08 コニカミノルタ株式会社 Image processing device, pathological diagnosis support system, image processing program, and pathological diagnosis support method
EP3364341A1 (en) * 2014-06-16 2018-08-22 Siemens Healthcare Diagnostics Inc. Analyzing digital holographic microscopy data for hematology applications
US10614287B2 (en) 2014-06-16 2020-04-07 Siemens Healthcare Diagnostics Inc. Virtual staining of cells in digital holographic microscopy images using general adversarial networks
JP6375925B2 (en) * 2014-12-15 2018-08-22 コニカミノルタ株式会社 Image processing apparatus, image processing system, image processing program, and image processing method
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WO2017212055A1 (en) 2016-06-10 2017-12-14 F. Hoffmann-La Roche Ag System for bright field image simulation
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JP6901072B2 (en) * 2017-08-30 2021-07-14 学校法人 埼玉医科大学 Image processing device, image processing method, image processing program
JP7200945B2 (en) * 2017-12-01 2023-01-10 ソニーグループ株式会社 Image coloring device, image coloring method, program, and image coloring system
WO2019118544A1 (en) * 2017-12-15 2019-06-20 Verily Life Sciences Llc Generating virtually stained images of unstained samples
US10665000B2 (en) 2018-01-08 2020-05-26 AcuSolutions Inc. Pseudo H and E image producing method and optical system using same
CA3095739A1 (en) 2018-03-30 2019-10-03 The Regents Of The University Of California Method and system for digital staining of label-free fluorescence images using deep learning
TWI683283B (en) 2018-04-03 2020-01-21 薩摩亞商銳準醫光股份有限公司 Image synthesis method of biological sample and optical system adopting the method
CN110346291A (en) * 2018-04-03 2019-10-18 锐准医光股份有限公司 A kind of image synthesis method of biological sample and optical system using this method
US20200388033A1 (en) * 2019-06-10 2020-12-10 Omics Data Automation, Inc. System and method for automatic labeling of pathology images
US11354804B1 (en) 2019-09-27 2022-06-07 Verily Life Sciences Llc Transforming multispectral images to enhanced resolution images enabled by machine learning
US11699220B2 (en) * 2019-10-02 2023-07-11 Chemimage Corporation Fusion of molecular chemical imaging with RGB imaging
JP2021124861A (en) * 2020-02-04 2021-08-30 ソニーグループ株式会社 Analysis device, analysis method, analysis program, and diagnosis support system
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Also Published As

Publication number Publication date
CA2773268A1 (en) 2011-04-07
CN102575990B (en) 2015-06-17
EP2483668A4 (en) 2017-11-15
WO2011040872A1 (en) 2011-04-07
US20110074944A1 (en) 2011-03-31
RU2012111453A (en) 2013-11-20
CN102575990A (en) 2012-07-11
KR20120079080A (en) 2012-07-11
AU2010301166A1 (en) 2012-03-29
JP5631999B2 (en) 2014-11-26
JP2013506129A (en) 2013-02-21
EP2483668A1 (en) 2012-08-08
US8269827B2 (en) 2012-09-18

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