TW201512648A - Intelligent and automated chemical detecting apparatus in identification and quantification and detecting method thereof - Google Patents

Abstract

An intelligent and automated chemical detecting apparatus in identification and quantification and a detecting method thereof are disclosed. The method includes steps: placing a to-be-tested object into a detection container, and conducting the detection through a solvent processing device, an extraction device, a concentration device, a detecting device and an electronic device, to obtain the chemicals of the to-be-tested object through a speedy identified and quantified approach.

Description

Detecting device and detecting method for qualitative and quantitative intelligent chemical substances

The invention relates to a detection device and a detection method for qualitative and quantitative intelligent chemical substances, in particular to a detection device and a method with simple operation and short detection time and high detection sensitivity.

Food is a necessity to maintain bodily functions and metabolism, usually composed of carbohydrates, fat, protein or water. In addition to maintaining physical function, food can also affect people's moods. For example, some foods contain substances that bring pleasure to people. With the improvement of the quality of life and the relatively busy work, people's pressure is gradually increasing. Therefore, today's food is not just for people to enjoy food and clothing. Instead, they enjoy the happiness and happiness brought by food. Therefore, today's catering industry is particularly prosperous, and a variety of snacks and beverages are also emerging, giving people more choices about food.

However, the continuous advancement of technology has also led to the prosperity of the food processing industry. In order to meet the needs of the market, the relevant industry will process natural food into food of higher commercial value, which may add chemicals to the food. Food is usually packaged for convenience, and the chemicals on the packaging Easy to stick to food. However, if people eat too much chemical additives, it will affect the body's function and cause disease. Basically, when people buy food, they can't directly observe the chemical substances left on the food through the naked eye. They can only rely on the government's control and the conscience of the industry. Inevitably, there will be unsafe foods that are sold in the market and eaten by consumers, such as toxic milk trimeric hydrogen and plasticizer storms. Some manufacturers are pursuing low-cost and legal food additives. In the use of cheap industrial plasticizers, the impact of a wide range, such as beverages, bread, cakes and even medicines, if accidentally eat foods with plasticizers, not only cause reproductive system lesions, and even carcinogenic danger. In recent years, China and Taiwan have been experiencing food toxic additive incidents such as tainted milk events (melamine, plasticizer, clenbuterol), carcinogenic soy sauce of monochloropropanediol, chlorpyrifos of maleic acid, and steroid (betamethasone). ), diuretic (hydrochlorothiazide), cardiotonic (caffeine) and other pigeon feed additives, Tianjin poisonous dumplings and environmental hormones that have not yet erupted, aquaculture, electronic consumer products, toys, daily necessities, cosmetics, agricultural products, etc., causing people Uneasy and paying attention to food inspection, rushing to buy milk tide panic and arousing the thorough inspection consciousness of various manufacturers; coupled with the large-scale use of pesticides by agricultural crops, agricultural administrative units have paid more and more attention to the control of pesticides in agricultural products; the feed in the aquaculture industry is frequently poisoned, causing export obstruction. The rivers and ecosystems of various countries have been polluted, the environmental monitoring and control has become more urgent and strict, and the reasons for rapid and simple testing have been comprehensively advanced to industrial production control purposes, and the sensitivity requirements for testing are becoming more and more traceable and reliable. need.

In addition, the medicines that are eaten and sick today and the health foods that maintain physiological functions are widely used in the market, and before various types of medicines are circulated in the market, The pharmaceutical company will conduct a rigorous test to check whether the ingredients of the drug are correct and carry out the final check.

In daily life, consumers often face the trouble of buying fakes and fake wines. The government and manufacturers are in difficulty, and consumers have the need to self-test.

Conventional techniques are used to test components in foods and pharmaceuticals by gas chromatography mass spectrometry (GC) or high performance liquid chromatography (HPLC). The disadvantage of gas chromatography mass spectrometry (GC) is that it takes time to increase the analysis. The volatility of the material, which in turn causes interference during the analysis and increases the difficulty of quantification, while the high performance liquid chromatography (HPLC) differs in the chemical or physical properties of the preparation process to separate the mixture. When using high performance liquid chromatography, the liquid analyte is injected into the column at the same time, mainly based on the difference in residence time caused by the difference in the interaction strength between the mobile phase and the stationary phase when the analyte is passed through the column. The separation is achieved, and finally the signal is detected by the detector's signal intensity of each substance, so that the analysis time is long (generally about one or two weeks or more) and the process is cumbersome. Moreover, high performance liquid chromatography does not recognize the properties of the sample by a detector such as an ultraviolet detector. In addition, high-performance liquid chromatography (HPLC) equipment and consumables are expensive, and a large amount of toxic organic solvent waste can cause great harm to the natural environment, strict operation, and method development is not simple and time-consuming. Therefore, the above two types of equipment need to be trained to operate the machine and have experience in application to be able to quickly apply to test the composition of food and medicine. For the pharmaceutical company, the above-mentioned lacking also leads to high production cost of the medicine.

The above inventors have invested a lot of time in relevant research, and compared various advantages and disadvantages, and carried out research and development of related products, and pushed The invention discloses a "detecting device and a detecting method for qualitative and quantitative intelligent chemical substances".

The main purpose of the invention is to achieve rapid (0.1 seconds to several minutes) and a large amount of analysis of toxic chemical solvents in the test object, thereby improving the sensitivity and accuracy of the analysis and detection, wherein the test object is a drug or a drug raw material. , food, food pollution, environmental hormones, explosives, viruses, cancer biomarkers, identification of genuine and fake items, identification of real wine and fake wine, etc., and the sensitivity and accuracy of the analysis and detection.

In order to achieve the above object and effect, the present invention is a first embodiment of a smart automatic chemical substance qualitative and quantitative detecting device, comprising: a container to be tested, which stores the object to be detected; a solvent processing device, The invention relates to a storage, heating and pressurizing solvent; an extracting device comprising an extracting machine, a perforating group, a switch member, a weight measuring unit and an extracting dropper, wherein the extracting machine is provided with a placing portion, and A detection channel communicating with the placement portion is disposed in the extraction machine, and the extraction device comprises a perforation group, a switch member, a weight measuring unit and an extraction dropper, wherein the perforation group is set in the detection channel. And the perforating group clamps and pierces the container to be tested, the switch member is pivotally disposed on the extraction device, and is disposed in conjunction with the perforation group, the weight measuring unit is corresponding to the placement portion and is disposed in the detection channel, and the extraction pipette assembly is set At the end of the extraction machine, and connected to the detection channel, the extraction pipette is provided with a titration end, and the solvent is mixed with the object to be detected through the extraction machine to form an extract, and the weight measurement unit outputs a a pipeline connecting the solvent treatment device and the detection channel of the extraction device; a concentration device comprising a moving platform and a collection container, the mobile platform being provided with a heating portion, the collection container being placed in the heating Department and heating and extraction The extracting liquid is concentrated into a concentrated liquid by a pipette blowing method; a detecting unit includes a sampler, a light focusing control group and a detecting carrier, the detecting carrier set is disposed on the mobile platform, and the detecting slide is The end surface is provided with a joint portion which is combined with the molecules in the concentrated liquid, and the light focus control group reads the light and then reads a Raman spectrum signal; and an electronic device electrically connected to the solvent treatment device, the extraction device and the concentrating device a detecting unit, the electronic device receives the Raman spectral signal to analyze the chemical substance contained in the detecting object, and the electronic device receives the weight information; accordingly, when the molecule of the concentrated liquid is combined with the detecting slide, the light focusing control group is Reading the highest Raman spectral signal, the weight measuring unit provides the weight information to the electronic device, and the electronic device performs the comparison analysis of the weight information of the object to be detected and the Raman spectral signal, and then recombines the chemical substance of the object to be detected, thereby achieving Qualitative and quantitative effects of the analyte, providing fast and easy to operate, fully automated and highly sensitive detection capabilities, allowing users to quickly A detection object to be detected chemical substances included, and to achieve quality control and testing of the qualitative and quantitative effects.

The first embodiment of the present invention further includes the following technical features: the solvent treatment device includes a reservoir for storing a solvent, a heating chamber for heating the solvent, two solenoid valves, and a pressure pump connected to the heating chamber, and the pipeline includes a front pipeline and a rear pipeline, the front pipeline communicates with the liquid storage bottle and the heating chamber, and the pipeline is connected to the heating chamber and the detection channel, and the two solenoid valves are respectively arranged on the front pipeline and the rear pipeline, The two solenoid valves adjust the liquid storage bottle and the heating chamber and the heating chamber and the detection passage to be in a conducting state or a blocking state, and the pressurized pump functions as a pushing solvent. According to a further feature of the first embodiment of the present invention, the perforating group comprises two oppositely disposed needle segments, and the shape of each needle segment The shape corresponds to the structure of the container to be tested, and each end of the needle piece has a plurality of needle tubes. When the two needle pieces are clamped to the sides of the container to be tested, the plurality of needle tubes of each needle piece will penetrate into the container to be tested.

According to a further feature of the first embodiment of the present invention, the bonding portion of the detecting carrier is a surface-enhanced Raman-active substrate, and the surface-enhanced Raman-active carrier is a sol of metal particles, and the surface of the bonding portion is provided. There is a Raman spectral focusing spot, which is a cymbal or other Raman spectroscopy standard for the application of concentrating focus setting, and a concentrated liquid limiting ring is arranged on the periphery of the joint ( Retaining Ring), the concentrate limiting ring is made of paraffin ring or Teflon to limit the concentration of concentrated droplets to the detection slide and maintain the uniformity and reliability of the concentrate distribution.

The light focus control group includes a light source device and a spectrometer capable of projecting laser light, ultraviolet light (UV), near infrared light (NIR), far infrared light (FIR), or visible light. The light source device includes a body, a light source, an light head, and a set of focus adjustment mechanisms disposed on the light head. The light source is disposed at one end of the body, and the light source projects at least one wavelength of light and generates at least a projection optical path and at least one return optical path, wherein the optical head is disposed at the other end of the body, the optical head corresponding to the detecting carrier; the at least one projection optical path sequentially passes through a first collimating lens, a laser line filter, and a color The filter and a beam splitter finally pass through the junction of the optical head to the detecting carrier, and the projection optical path generates a first focusing optical path through the beam splitter to the detecting carrier, and is split. a second focusing optical path reflected by the device, the second focusing optical path projected through a second focusing mirror to a group of the body a photosensitive element; the bonding portion of the at least one return light route detecting carrier sequentially passes through the optical head, the color filter, a reflective sheet, a second collimating lens and a set of long-pass filters disposed on the inner wall of the body The long-pass filter is electrically connected to the spectrometer; the focal length adjusting mechanism, the photosensitive element, and the spectrometer are electrically connected to the electronic device; the electronic device compares the path length of the first focusing optical path with the path length of the second focusing optical path The difference is that the focal length automatic adjustment mechanism adjusts the focal length of the optical head and the detecting slide to adjust the focal length of the optical head.

A further technical feature of the first embodiment of the present invention further includes a light focus control device electrically connected to the light source, and the light source is provided with at least two light emitting patterns, and the light focus control device controls the light emitting state of any of the light emitting patterns And the illuminating wavelength, the illuminating source projects the light of the at least one wavelength, and controls the wavelength, power, light intensity and illuminating state of the illuminating crystal grain.

According to a further feature of the first embodiment of the present invention, the electronic device includes a data storage unit, a receiving unit, and a processing unit. The data storage unit and the receiving unit and the processing unit exchange data with each other, and the data storage unit stores the plurality of sample weight information. , Raman spectral information of complex samples and scattering intensity values of complex samples, according to which the processing unit uses the weight information of the object to be identified, the relative relationship between Raman spectral information and scattering intensity values, the Least Square Method and the full limit. Data analysis of the Full Constrained Least Square Method, comparing the weight information of the plurality of samples in the data storage unit, the Raman spectrum information of the complex sample, and the scattering intensity values of the complex sample for baseline correction, fluorescence spectroscopy The subtraction, and the data qualitative and quantitative analysis of the chemical substances contained in the test substance.

According to a further feature of the first embodiment of the present invention, the mobile platform includes a plurality of displacement motors that provide multi-directional displacement and rotation of the moving platform; the sampler includes a plurality of moving motors and a sampler, the plurality of moving motors providing samplers Multi-directional displacement and rotation, and the sampler is a Syringe, a Pipette or a Motorized Auto-Pipette.

The first embodiment of the invention relates to a method for detecting qualitative and quantitative intelligent chemical substances, which comprises the following steps: sample positioning: the container to be tested equipped with the object to be tested is put into an extraction device, and the object to be tested is located in the weight Measuring unit, and generating weight information by the weight measuring unit, and then operating the switch member to clamp the perforation group and partially penetrate the container to be tested; solvent treatment: heating the solvent in the solvent treatment device (10C to 200C) and pressurizing (0.1kg/cm2 to 1000kg/cm2 to the extraction device; extraction: the solvent flows into the container to be tested and mixed with the analyte to produce an extract, and the extraction pipette drops the collected extract into the collection container; Heating and blowing to accelerate the evaporation of the solvent in the collection container to obtain a concentrated liquid; analysis: the sampler draws the concentrated liquid, drops it to the test slide and combines with the joint to blow dry, and then irradiates the light focus control group to read Raman The spectral signal is input to the electronic device for analysis, and the electronic device analyzes the Raman spectral signal and weight information to analyze the chemical substance contained in the object to be detected.

The first embodiment of the method for detecting qualitative and quantitative intelligent chemical substances of the present invention further includes the following technical features. In the analyzing step, the electronic device stores the Raman spectral signal and weight information of the object to be detected in the electronic device. The Raman spectral signal of the complex sample, the weight information of the plurality of samples for comparison, the baseline correction, the subtraction of the fluorescence spectrum, Data qualitative and quantitative analysis, using the Least Square Method and the Full Constrained Least Square Method data to analyze the chemicals contained in the analyte.

According to a further feature of the first embodiment of the present invention, the light focus control group emits the first light and the second light, and the first light and the second light have similar wavelengths, thereby generating a first spectrum when scanning and detecting the object to be detected. The second spectrum is used to obtain the qualitative and quantitative results of the dark sample by subtraction and baseline correction of the fluorescence spectrum, or the light focus control group emits a far infrared light, thereby generating a scan by detecting the object to be detected by scanning The Raman spectrum of the fluorescent noise is used to achieve qualitative and quantitative results for detectable dark samples.

According to a further feature of the first embodiment of the present invention, in the solvent treatment step, the pressurized solvent is used to push the heated solvent from the solvent treatment device to the extraction device, and the pressure pump generates 0.1 kg/cm ² to 1000 kg/cm ^{2 .} The pressure of the range; the solvent is an organic solvent, an aqueous solvent, a mixed solvent or carbon dioxide.

A second embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention comprises: a container to be tested for storing the object to be detected; and a solvent processing device for storing and heating the pressurized solvent; An extraction device comprising an extraction machine, a perforation group, a switch member and a weight measuring unit, the extraction machine is provided with a placement portion, and the extraction machine is provided with a detection channel communicating with the placement portion and a An outlet end that communicates with the detection side channel, the perforation group active group is disposed in the detection channel, and the perforation group clamps against and pierces the container to be tested, and the switch member is pivotally disposed on the extraction machine and is disposed in conjunction with the perforation group. The weight measuring unit is corresponding to the placing portion and is disposed on the detecting channel, and the outlet end is connected to the detecting channel, and the heated solvent is introduced into the detecting channel by the pipeline, and the object to be detected in the container to be tested is dissolved through the punching group to generate an extract. And the weight measuring unit outputs a weight information; a pipeline connecting the solvent treatment device and the detection channel of the extraction device; and a detection tube set at the outlet end Communication channels with the detection, and detection The inner tube of the test tube is provided with a joint portion, and the extract liquid is connected to the test tube to be combined with the joint portion; a detecting unit comprising a light focus control group, the light focus control group for reading a Raman spectrum after projecting light to the joint portion The signal is used to set the focal length of the strongest signal and the Raman spectrum signal of the detecting object; an electronic device electrically connected to the solvent processing device, the extracting device and the detecting unit, the electronic device receiving the Raman spectral signal and analyzing the Raman spectral signal to Obtaining information on the chemical substance contained in the test object, and the electronic device receives the weight information; accordingly, the concentrated liquid is obtained by the solvent treatment device and the extraction device, and the combination of the molecules of the concentrated liquid and the detection tube is combined The light focus control group reads the weight information provided by the Raman spectrum signal and the weight measuring unit to the electronic device, and the electronic device recombines the object to be detected by comparing the analysis unit and analyzing the weight information of the object to be detected and the Raman spectrum signal. Chemicals to achieve qualitative and quantitative effects of the analyte, providing fast and easy operation, fully automated and sensitive High detection capabilities, allowing users to quickly detect chemical substances to be included in the test object and achieve the effect of quality control and testing.

The second embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features, the solvent processing device comprising a pressurizing pump, a liquid storage bottle, and a heating connected to the liquid storage bottle and the detecting passage a pipeline, the pipeline includes a first pipeline and a second pipeline, the first pipeline is connected to the pressure pump and the detection channel, and the second pipeline is connected to the liquid storage bottle and the detection channel, and is connected to the first pipeline A first valve is provided and a second valve is disposed on the second line.

The second embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features. The perforating group includes two oppositely disposed needle segments, and the shape of each needle segment corresponds to the structure of the container to be tested, and each The end surface of the needle piece has a plurality of needle tubes. When the two needle pieces are clamped to the two sides of the container to be tested, the plurality of needle tubes of each needle piece will penetrate into the container to be tested.

The second embodiment of the qualitative and quantitative detection device for the intelligent automatic chemical substance of the present invention further includes the following technical features, wherein the junction of the test tube is a surface-enhanced Raman-active substrate (SERS-active substrate), the surface-enhanced Raman activity The carrier is a sol of metal particles.

The second embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features. The light focusing control group includes a light source device and a spectrometer, and the light source device can project laser light and ultraviolet light (UV). a near-infrared (NIR), far infrared (FIR) or visible light, the light source device includes a body, an illumination source, an optical head, and a set of focal length adjusting mechanisms disposed on the optical head. The light source is disposed at one end of the body, the light source projects at least one wavelength of light, and generates at least one projection light path and at least one return light path, and the light head is disposed at the other end of the body, and the light head corresponds to the detection tube a joint portion; the at least one projection optical path is sequentially passed through a first collimating lens, a laser line filter, a color filter, a beam splitter, and finally through a joint of the optical head to the joint of the detecting tube, and the The projection optical path is generated by the beam splitter to generate a first focusing optical path through a combination of the beam splitter and the detecting tube, and a second focusing optical path reflected by the beam splitter, the second pair The light path is projected through a second focusing mirror to a group of photosensitive elements disposed on the body; the junction of the at least one return light routing detection tube sequentially passes through the optical head, the color filter, a reflective sheet, and a second collimating lens. And a set of long-pass filters disposed on the inner wall of the body, the long-pass filter is electrically connected to the spectrometer; the focal length adjusting mechanism, the photosensitive element and the spectrometer are electrically connected to the electronic device; and the electronic device is in comparison The focal length adjustment mechanism automatically adjusts the focal length of the optical head to adjust the focal length of the joint between the optical head and the detection tube.

The second embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features, further comprising a light focusing control device electrically connected to the light emitting source, wherein the light emitting source is provided with at least two light emitting crystal grains, The light focus control device regulates Light-emitting state and light-emitting wavelength of any of the light-emitting crystal grains, causing the light-emitting source to project the light of the at least one wavelength, and controlling the wavelength, power, light intensity and light-emitting state of the light-emitting die; the light focus control group is provided with a rotating motor, The rotating motor drives the light source device to displace.

A second embodiment of the method for detecting qualitative and quantitative intelligent chemical substances of the present invention comprises the following steps: sample positioning: putting a container to be tested containing the object to be tested into an extraction device, and then operating the switch member to clamp the perforation group The container to be tested is partially penetrated into the container to be tested; solvent treatment: heating and pressurizing the solvent in the solvent treatment device to the extraction device; extracting: the solvent flows into the container to be tested and mixed with the analyte to produce an extract; Analysis: The extract is sent from the outlet end to the test tube, and the molecules in the extract are combined with the joint, heated and blown dry, and then a Raman spectrum signal is read by the light focus control group and input into the electronic device for analysis. The electronic device compares the Raman spectral signal and weight information to analyze the chemical substances contained in the object to be detected.

The second embodiment of the method for detecting a qualitative and quantitative intelligent chemical substance of the present invention further includes the following technical features: in the analyzing step, the electronic device stores the Raman spectral signal and weight information of the object to be detected and stored therein. Complex sample Raman spectroscopy signal, complex sample weight information for comparison, baseline correction, fluorescence spectral subtraction, data qualitative and quantitative analysis, application of least square error method (Least Square Method) and fully restricted condition least square error method ( The data of Full Constrained Least Square Method) analyzes the chemical substances contained in the analyte.

In a second embodiment of the method for detecting a qualitative and quantitative intelligent chemical substance of the present invention, the light focusing control group emits a first light and a second light, wherein the first light and the second light have similar wavelengths, thereby scanning detection When the object to be detected is generated, the first spectrum is generated and The second spectrum is used to obtain the qualitative and quantitative results of the dark sample by subtraction and baseline correction of the fluorescence spectrum, or the light focus control group emits a far infrared light, thereby generating a scan by detecting the object to be detected by scanning The Raman spectrum of the fluorescent noise is used to achieve qualitative and quantitative results for detectable dark samples.

The method for detecting a qualitative and quantitative detection device for an intelligent automatic chemical substance according to the present invention, in the sample positioning step, the weight measuring unit measures the weight data of the object to be tested into the electronic device.

The method for testing the qualitative and quantitative detection device of the intelligent automatic chemical substance of the invention, in the solvent treatment step, opening the second valve to transfer the solvent in the liquid storage bottle to the heating chamber for heating (10 C to 200 C); the solvent is an organic solvent , water, mixed solvents or carbon dioxide.

In the method for detecting qualitative and quantitative intelligent chemical substances in the invention, in the extraction step, the first valve is opened to allow the gas in the pressure pump to flow into the detection channel, and the solvent is pushed into the container to be tested, and finally to the test tube. The pressure of the gas sent by the pressure pump is 0.1 kg/cm ² to 1000 kg/cm ² .

A third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention comprises: a container to be tested for storing the object to be detected; and a solvent processing device for storing, heating and pressurizing the solvent An extraction device comprising an extraction machine, a perforation group, a switch member, a weight measuring unit and an extraction dropper, the extraction machine having a placement portion and a connection with the placement portion therein The detection channel, the perforation group activity group is disposed in the detection channel, and the switch component is arranged in linkage with the perforation group, the weight measurement unit is corresponding to the placement portion and is disposed in the detection channel, and the extraction dropper group is disposed at the end of the extraction machine and Connected to the detection channel, the extraction pipette is provided with a titration end, and the solvent is mixed with the object to be detected through the extraction device to form an extract, and the weight The measuring unit outputs a weight information; a pipeline connecting the solvent processing device and the detection channel of the extraction device; a concentrating device comprising a moving platform and a detecting container, the moving platform is provided with a heating portion, the detecting container Placed in the heating part, and the detection container has a joint portion, the extract is in the detection container, and is concentrated into a concentrated liquid by heating and blowing, and the molecules in the concentrated liquid are combined with the joint; a detecting unit includes a a light focus control group, the light focus control group includes a light source device, the light source device is provided with an optical head at one end, the light head is disposed through the moving platform and corresponding to the joint portion of the detecting container, and the light focusing control group projects the light to the joint portion Reading a Raman spectral signal; an electronic device electrically connected to the solvent processing device, the extraction device, the concentrating device, the detecting unit, and the electronic device receiving the chemical substance contained in the Raman spectroscopy signal, and receiving the electronic device The weight information.

The third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features, the solvent processing device includes a liquid storage bottle for storing a solvent, a heating chamber for heating the solvent, two electromagnetic valves, and a a heating pump connected to the heating chamber, and the pipeline includes a front pipeline and a rear pipeline, the front pipeline communicates with the liquid storage bottle and the heating chamber, and the pipeline is connected to the heating chamber and the detection channel, and the two solenoid valves respectively The group is arranged on the front pipeline and the rear pipeline, and the two solenoid valves adjust the liquid storage bottle and the heating chamber, the heating chamber and the detection passage to be in a conducting state or a blocking state, and the pressure pump is used as a function of pushing the solvent.

The third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features. The perforating group includes two oppositely disposed needle segments, and the shape of each needle segment corresponds to the structure of the container to be tested, and each The end surface of the needle piece has a plurality of needle tubes. When the two needle pieces are clamped to the two sides of the container to be tested, the plurality of needle tubes of each needle piece will penetrate into the container to be tested.

The third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features, the bonding portion of the detecting container is a surface-enhanced Raman-active substrate (SERS-active substrate), and the surface-enhanced Raman-active carrier It is a sol body of metal particles.

The third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features. The light focusing control group includes a light source device and a spectrometer, and the light source device can project laser light and ultraviolet light (UV). a near-infrared (NIR), far infrared (FIR) or visible light, the light source device includes a body, an illumination source, an optical head, and a set of focal length adjusting mechanisms disposed on the optical head. The light source is disposed at one end of the body, the light source projects at least one wavelength of light, and generates at least one projection light path and at least one return light path, and the light head is disposed at the other end of the body, and the light head corresponds to the detection load. a film; the at least one projection optical path sequentially passes through a first collimating lens, a laser line filter, a color filter, a beam splitter, and finally passes through the optical head to the joint of the detecting carrier, and the projection optical path is divided into The beam generator generates a first focusing optical path through the beam splitter to the detecting carrier, and a second focusing optical path reflected by the beam splitter, the second focusing optical path is projected through a second focusing a pair of photosensitive elements disposed on the body; the bonding portion of the at least one return light route detecting carrier sequentially passes through the optical head, the color filter, a reflective sheet, a second collimating lens, and a group disposed in the body a long-pass filter of the wall, the long-pass filter is electrically connected to the spectrometer; the focal length adjusting mechanism, the photosensitive element and the spectrometer are electrically connected to the electronic device; and the electronic device compares the path length of the first focusing optical path and The difference in the path length of the second focusing optical path is adjusted by the focal length adjusting mechanism to adjust the focal length of the optical head to adjust the focal length of the optical head and the detecting container.

Detecting device for qualitative and quantitative detection of intelligent automatic chemical substances of the invention The third embodiment further includes the following technical features, further comprising: a light focusing control device electrically connected to the light source, wherein the light source is provided with at least two light emitting patterns, and the light focusing control device regulates the light emitting state of any of the light emitting crystal grains And the illuminating wavelength, the illuminating source projects the light of the at least one wavelength, and controls the wavelength, power, light intensity and illuminating state of the illuminating crystal grain.

The third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention further includes the following technical features, the electronic device comprising a data storage unit, a receiving unit and a processing unit, the data storage unit and the receiving unit and the processing The unit mutual data transmission, the data storage unit stores the plurality of sample weight information, the complex sample Raman spectrum information and the plurality of sample scattering intensity values, according to which the processing unit uses the weight information of the object to be identified, the Raman spectrum information and the relative value of the scattering intensity values. Data analysis of the relationship, the Least Square Method and the Full Constrained Least Square Method, comparing the weight information of the plurality of samples in the data storage unit, and the Raman spectrum information of the complex sample And the complex sample scattering intensity values are subjected to baseline correction, subtraction of the fluorescence spectrum, and data qualitatively and quantitatively analyzing the chemical substances contained in the detection object.

The third embodiment of the method for detecting qualitative and quantitative intelligent chemical substances of the present invention comprises the following steps: sample positioning: the container to be tested equipped with the object to be tested is put into the extraction device, and the object to be tested is located in the weight measuring unit. The weight measuring unit generates a weight information, and then operates the switch member to clamp the perforated group and partially penetrate the container to be tested; solvent treatment: heating and pressurizing the solvent in the solvent treatment device to the extraction device; and extracting: The solvent flows into the container to be tested to mix with the analyte and produces an extract, and the extraction pipette drops the collected extract into the detection container; concentrates: heats and blows the detection solution in the container to volatilize the solvent. a concentrated liquid; analysis: after the light focus control group detects the junction of the container after irradiation, reading the Raman light The spectral signal is input to the electronic device for analysis, and the electronic device analyzes the Raman spectral signal and weight information to analyze the chemical substance contained in the object to be detected.

The third embodiment of the method for detecting qualitative and quantitative intelligent chemical substances of the present invention further includes the following technical features; in the analyzing step, the electronic device compares the Raman spectral signal of the object to be detected, the weight information, and the plurality of samples stored therein. Raman spectral signal, complex sample weight information for comparison, baseline correction, fluorescence spectral subtraction, data qualitative and quantitative analysis, application of least square error method (Least Square Method) and fully constrained least square error method (Full Constrained The data of Least Square Method) analyzes the chemicals contained in the analyte.

In a third embodiment of the method for detecting qualitative and quantitative intelligent chemical substances, the light focusing control group emits a first light and a second light, and the first light and the second light have relatively close wavelengths, so that scanning detection is performed. When the object is detected, a first spectrum image and a second spectrum image are generated, thereby performing qualitative and quantitative detection of the dark sample by subtracting the fluorescence spectrum and correcting the baseline.

A third embodiment of the qualitative and quantitative detection method for the intelligent automatic chemical substance of the present invention, in the solvent treatment step, the pressurized solvent is used to push the heated solvent from the solvent treatment device to the extraction device, and the pressure pump generates 0.1 kg. a pressure in the range of /cm ² to 1000 kg/cm ² ; the solvent is an organic solvent, an aqueous solvent, a mixed solvent or carbon dioxide.

1‧‧‧ Container to be tested

2‧‧‧Solvent treatment unit

21‧‧‧Liquid bottle

22‧‧‧heating room

23‧‧‧ solenoid valve

24‧‧‧ solenoid valve

25‧‧‧Pressure pump

3‧‧‧ Extraction device

31‧‧‧Placement Department

32‧‧‧Detection channel

33‧‧‧Perforation group

331‧‧‧ Needle tube

3311‧‧‧ needle

34‧‧‧Switches

35‧‧‧ Weight measuring unit

36‧‧‧ Extraction dropper

361‧‧‧Trip end

37‧‧‧export end

38‧‧‧Extraction machine

4‧‧‧pipe

41‧‧‧ Front pipeline

42‧‧‧After the pipeline

43‧‧‧First line

431‧‧‧First valve

44‧‧‧Second line

441‧‧‧Second valve

5‧‧‧Concentrated device

51‧‧‧Mobile platform

511‧‧‧displacement motor

52‧‧‧Collection container

53‧‧‧ heating department

6‧‧‧Detection unit

61‧‧‧Sampling device

611‧‧‧Moving motor

612‧‧‧sampler

62‧‧‧Light Focus Control Group

621‧‧‧Light source device

6211‧‧‧Light source

62111‧‧‧Lighting grain

6213‧‧‧Out of the head

6214‧‧•focal length adjustment mechanism

6215‧‧‧Light path

6216‧‧‧Return light path

6217‧‧‧First collimating lens

6218‧‧‧Laser line filter

6219‧‧‧Color filters

6220‧‧‧beam splitter

6221‧‧‧First focusing light path

6222‧‧‧Second focusing light path

6224‧‧‧Photosensitive elements

6225‧‧‧reflector

6226‧‧‧Second collimating lens

6227‧‧‧Long pass filter

622‧‧‧ Spectrometer

623‧‧‧Light focus control device

63‧‧‧Test slides

631‧‧‧Combination Department

632‧‧‧Concentration limit ring

633‧‧‧Raman Spectral Concentration Focus

7‧‧‧Electronic devices

71‧‧‧Data storage unit

72‧‧‧ receiving unit

73‧‧‧Processing unit

8‧‧‧Test tube

81‧‧‧Combination Department

9‧‧‧Test container

91‧‧‧Combination Department

The first figure is a schematic view of a device of a first embodiment of the detecting device of the present invention.

The second to fifth figures are diagrams showing the operation of the first embodiment of the detecting device of the present invention.

Figure 6 is an enlarged schematic view showing a solvent treatment device and an extraction device of a first embodiment of the detecting device of the present invention.

The seventh drawing is an enlarged schematic view of the concentrating apparatus of the first embodiment.

The eighth figure is an enlarged schematic view of the detecting unit.

The ninth and tenth drawings are diagrams of the second embodiment of the detecting device of the present invention.

Figure 11 is a view showing the apparatus of the third embodiment of the detecting device of the present invention.

Figure 12 is an enlarged schematic view showing a concentrating device and a detecting unit of the third embodiment of the detecting device of the present invention.

The thirteenth diagram is a schematic diagram of a light source device.

The fourteenth figure is an electronic device diagram.

The fifteenth diagram is an enlarged view of the cross section of the test slide.

Figure 16 is a flow chart showing the steps of the first and third embodiments of the detection method of the present invention.

Figure 17 is a spectrum diagram of the present invention for detecting melamine using a 785 nm laser wavelength.

Figure 18 is a graph showing the sensitivity of the present invention with different concentrations of melamine.

The nineteenth figure is a spectrogram comparing the intensity of the melamine peak at 675.9 nm and the intensity of the Raman spectrum at 520 nm to achieve the best focus distance.

Figure 20 is a flow chart showing the steps of the second embodiment of the detecting method of the present invention.

In order to achieve the above objects and effects, and the technical means and configurations employed, the features and effects of the embodiments of the present invention are described in detail with reference to the accompanying drawings.

As shown in the first to fifth figures, a first embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention comprises: a container 1 to be tested, which stores the object to be detected; a solvent The processing device 2 is a storage, heating and pressurizing solvent (the stainless steel, the glass, the capsule type and the like are used as the container 1 to be tested in the embodiment, and the object to be detected may be the food and the medicine processed by crushing, grinding, etc. An extraction device 3, comprising an extraction machine 38, a perforation group 33, a switch member 34, a weight measuring unit 35 and an extraction drip tube 36. The extraction machine 38 is provided with a placement portion 31, and A detection channel 32 is formed in the extraction machine 38. The perforation group 33 is movably disposed in the detection channel 32, and the perforation group 33 is clamped and pierces the container 1 to be tested. It is pivotally disposed on the extraction machine 38 and is disposed in conjunction with the perforation group 33. The weight measuring unit 35 is corresponding to the placement portion 31 and is disposed on the detection channel 32, and the extraction drip tube 36 is disposed at the end of the extraction machine 38, and In communication with the detection channel 32, the extraction dropper 36 is provided with a titration end 361. The agent is mixed with the object to be detected via the extraction machine 38 to form an extract, and the weight measuring unit 35 outputs weight information; a pipe 4 that communicates with the solvent processing device 2 and the detection channel 32 of the extraction device 3; The device 5 includes a moving platform 51 and a collecting container 52. The moving platform 51 is provided with a heating portion 53. The collecting container 52 is placed on the heating portion 53, and the extract is accelerated by heating and extracting the dropper 36 to blow air. Volatilization of the solvent to increase the concentration of the extract into a concentrate; a detecting unit 6 comprising a sampling device 61, a light focusing control group 62 and a detecting carrier 63, the detecting carrier 63 being assembled on the moving platform 51 And the end surface of the detecting slide 63 is provided with a joint portion 631, and the joint portion 631 is combined with the molecules in the concentrated liquid, and the light focus control group 62 reads the light and reads a Raman spectrum signal; An electronic device 7, the electronic device 7 of the present invention may be a computer device or other electronic product, which is electrically connected to the solvent processing device 2, the extraction device 3, the concentrating device 5, the detecting unit 6, and the electronic device 7 receives the Raman spectral signal analysis. The chemical substance contained in the sample is detected while the electronic device 7 receives the weight information.

The second embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention, as shown in the ninth and tenth drawings, comprises: a container to be tested 1 for storing the object to be detected; a solvent treatment The device 2 is a storage and heating solvent; an extraction device 3, the extraction device 3 is provided with a placement portion 31, and the extraction device 3 is provided with a detection channel 32 communicating with the placement portion 31, and the extraction device 3 includes a a punching group 33, a switch member 34, a weight measuring unit 35, and an outlet end 37. The punching group 33 is movably disposed in the detecting channel 32, and the punching group 33 is clamped against and pierces the container 1 to be tested. 34 is pivotally disposed in the extraction device 3, and is disposed in conjunction with the perforating group 33. The weight measuring unit 35 is disposed corresponding to the placing portion 31 and disposed in the detecting passage 32, and the outlet end 37 is in communication with the detecting passage 32, and the heated solvent is The pipeline 4 is introduced into the detection channel 32, and dissolves the analyte in the container 1 to be tested through the perforation group 33 to produce an extract, and the weight measuring unit 35 outputs weight information; a pipeline 4, which communicates with the solvent treatment device 2 and the detection channel 32 of the extraction device 3 a test tube 8 is disposed at the outlet end 37 and communicates with the detection channel 32, and a detection portion 81 is disposed in the inner tube of the detection tube 8, and the extract liquid is introduced into the detection tube 8 to be combined with the joint portion 81; 6. It includes a light focus control group 62. The light focus control group 62 projects light to the joint portion 81 and reads a Raman spectrum signal. An electronic device 7 is electrically coupled to the solvent treatment device 2 and the extraction device 3. a detecting unit 6, the electronic device 7 receives the Raman spectrum signal, and analyzes the Raman spectrum signal to obtain a check The chemical substance information contained in the sample is detected, and the electronic device 7 receives the weight information.

Accordingly, the concentrate is obtained by the solvent treatment device 2 and the extraction device 3, and after the molecules of the concentrate are combined with the joint 81 of the test tube 8, the Raman spectrum signal and the weight measuring unit are read via the light focus control group 62. 35, the weight information provided to the electronic device 7, the electronic device 7 analyzes the weight information of the object to be detected and the Raman spectrum signal, and then recombines the chemical substance of the object to be detected to achieve the qualitative and quantitative effect of the object to be detected Provides fast and easy operation, fully automated and highly sensitive detection function, allowing users to quickly detect the chemical substances contained in the analyte and achieve quality control and detection (second embodiment and The difference between an embodiment is that the present invention can be used to qualitatively and quantitatively analyze the molecules of the extract by simply combining the molecules in the extract with the test tube 8 through the test tube 8 installed at the end of the extraction device 3. The chemical composition does not require the concentrating device 5, so other devices and structures are the same as those of the first embodiment, and the description thereof will not be repeated.

The third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device of the present invention, as shown in the eleventh and twelfth drawings, includes a container 1 to be tested, which stores the object to be tested; The solvent treatment device 2 is a storage, heating and pressurizing solvent; an extraction device 3 comprising an extraction machine 38, a perforation group 33, a switch member 34, a weight measuring unit 35 and an extraction drip tube 36, The extraction machine 38 is provided with a placement portion 31, and the detection machine 38 is provided with a detection channel 32 communicating with the placement portion 31. The perforation group 33 is movably disposed in the detection channel 32, and the perforation group 33 is clamped. And piercing the container 1 to be tested, the switch member 34 is pivotally disposed on the extraction device 3, and is disposed in conjunction with the perforation group 33. The weight measurement unit 35 is corresponding to the placement portion 31 and is disposed on the detection channel 32, and the extraction pipette 36 sets are arranged at the end of the extraction machine 38 and connected to the detection channel 32 The extraction pipette 36 is provided with a titration end 361, the solvent is mixed with the object to be detected by the extraction device 3 to form an extract, and the weight measuring unit 35 outputs a weight information; a pipe 4, which is connected to the solvent treatment a detection channel 32 of the device 2 and the extraction device 3; a concentrating device 5 comprising a moving platform 51 and a detecting container 9, the moving platform 51 is provided with a heating portion 53, the detecting container 9 is placed on the heating portion 53, and The detecting container 9 has a joint portion 91. The extract liquid is in the detecting container 9, and is concentrated into a concentrated liquid by heating and blowing, and the molecules in the concentrated liquid are combined with the joint portion 91. A detecting unit 6 includes a light focusing unit. The control unit 62 includes a light source device 621 (the light source device 621 is a laser head in the embodiment). The light source device 621 is provided with an optical head 6213 at one end, and the optical head 6213 is disposed on the mobile platform 51. Corresponding to the joint portion 91 of the detecting container 9, the light source device 621 projects light through the optical head 6213 to the joint portion 91 to read a Raman spectrum signal; an electronic device 7 electrically connected to the solvent processing device 2, the extraction device 3, Concentrator 5 The detecting unit 6 receives the chemical substance contained in the detection object by the Raman spectrum signal, and the electronic device 7 receives the weight information.

Accordingly, the extract liquid is obtained by the solvent treatment device 2 and the extraction device 3, and the molecules of the extract are combined with the joint portion 91 of the detection container 9, and the extract is concentrated into a concentrate by heating and blowing, and then The light focus control group 62 reads the weight information provided by the Raman spectrum signal and the weight measuring unit 35 to the electronic device 7, and the electronic device 7 analyzes the weight information and the Raman spectrum signal of the object to be detected, and then recombines the object to be detected. Chemicals to achieve qualitative and quantitative effects of the analyte, providing fast and easy to operate, fully automated and highly sensitive detection capabilities, allowing users to quickly detect the chemical substances contained in the analyte and achieve quality control And the efficacy of the test, the above is an example of a low concentration liquid sample.

In the third embodiment of the present invention, when detecting a high-concentration liquid or a powdery solid sample, it is placed in a detection container 9 with a high-concentration liquid or a powdery solid analyte to be measured, and the light source device 621 is automatically The focus is positioned on the joint portion 91 of the detection container 9 and the Raman spectrum is read, and the pull spectrum (Raman Spectrum) is input to the electronic device 7.

In addition, the first embodiment, the second embodiment, and the third embodiment of the intelligent automatic chemical substance qualitative and quantitative detecting device further include the following technical features: (1) Please refer to the second figure, the solvent processing device 2 The invention comprises a liquid storage bottle 21 for storing a solvent, a heating chamber 22 for heating the solvent, two electromagnetic valves 23 and 24, and a pressure pump 25 connected to the heating chamber 22 (the first and third embodiments of the present invention are the pressure pump 25 The pump, the second embodiment of the pressure pump 25 is a pneumatic cylinder), and the pipeline 4 includes a front line 41 and a rear line 42 that communicates with the liquid storage bottle 21 and the heating chamber 22, and then the pipeline 42 is connected to the heating chamber 22 and the detecting passage 32. The two solenoid valves 23 are respectively disposed on the front pipeline 41 and the rear pipeline 42. The two solenoid valves 23 adjust the liquid storage bottle 21, the heating chamber 22 and the heating chamber 22 And the detecting passage 32 is in an on state or a blocking state, and the pressure pump 25 starts to generate pressure to push the solvent through the pipeline 4 to the detection passage 32, and finally mixes with the detection substance in the container 1 to be tested, and then pushes the extract liquid. The function of extracting the dropper 36 to the outside is additionally used, and when the solenoid valve 23 provided in the front line 41 is closed State, the pressurizing pump 25 can be actuated to promote the effect of the gas through the heating chamber 22 in the line 42, the detection channel 32 to the dropper 36 is extracted outside, in order to achieve blowing. In the second embodiment of the present invention, the pipeline 4 includes a first conduit 43 and a second conduit 44. The first conduit 43 communicates with the pressure pump 25 and the detection passage 32, and the second conduit 44 communicates with the reservoir. The liquid bottle 21 and the detection passage 32 are provided with a first valve 431 on the first line 43 and a first line on the second line 44. Two valves 441.

(2) Referring to the second and seventh figures, the detailed structure of the perforating group 33 is further described. The perforating group 33 includes two oppositely disposed needle segments 331 and is placed in the detecting channel when the container 1 to be tested is placed from the placing portion 31. At 32 o'clock, the two needle segments 331 are respectively located at the front and rear end faces of the container 1 to be tested, and the shape of each of the needle segments 331 corresponds to the structure of the container 1 to be tested, and each end surface of each of the needle segments 331 has a plurality of needle tubes 3311, when the two needle tubes When the piece 331 is clamped to the two sides of the container 1 to be tested, the plurality of needle tubes 3311 of each of the needle pieces 331 penetrate into the container 1 to be tested, so that the inside of the container 1 to be tested and the detection channel 32 communicate with each other.

(3) Referring to the first, ninth, eleventh, and thirteenth drawings, the light focus control group 62 includes a light source device 621 and a spectrometer 622 (this embodiment is a Raman spectrometer), and the light source device 621 can project Laser light, ultraviolet light (UV), near infrared light (NIR), far infrared light (FIR), or visible light, and the present embodiment is laser light. The light source device 621 includes a light source 6211. An optical head 6213, a set of focal length adjusting mechanism 6214 disposed on the optical head 6213, the light source 6211 is disposed at one end of the light source device 621, and the light source 6211 projects at least one wavelength of light (for example, 785 nm or 638 nm, or 532 nm). And generating at least one projection optical path 6215 and at least one return optical path 6216, and the optical head 6213 is disposed at the other end of the light source device 621, the optical head 6213 corresponding to the detecting carrier 63; the at least one projection optical path 6215 is sequentially passed through The first collimating lens 6217, a laser line filter 6218, a color filter 6219 (inclined at 45 degrees), a beam splitter 6220 (T95/R5 beam splitter 6220, inclined at a negative 45 degrees) finally passes through the optical head 6213 (the optical head 6213 is provided with a focusing lens) to the detecting carrier 63 The combining portion 631, the projection optical path 6215 generates a first focusing optical path 6221 through the beam splitter 6220 to the detecting carrier 63 at the beam splitter 6220 (T95/R5 beam splitter 6220), and a beam splitter 6220. a second focusing light path 6222 that reflects The second focusing optical path 6222 is projected through a second focusing mirror 6223 to a group of photosensitive elements 6224 (photosensitive elements 6224 are photodiodes) disposed on the light source device 621 (the light of the at least one wavelength passes through the beam splitter 6220, 95 % of the light energy enters the first focusing light path 6221, and 5% of the light energy of the at least one wavelength enters the second focusing light path 6222). The angle between the first focusing light path 6221 and the second focusing light path 6222 is a right angle and is transmitted through The electronic device 7 adjusts the focal length by the focal length adjusting mechanism 6214 (voice coil motor) by comparing the path length of the first focusing optical path 6221 and the path length of the second focusing optical path 6222, and the focal length ranges from 5 mm to 8 mm. The focal length adjusting mechanism 6214, the photosensitive element 6224 and the spectrometer 622 are electrically connected to the electronic device 7; the at least one return optical path 6216 is sequentially passed through the combining portion 631 of the detecting carrier 63 through the optical head 6213, the color filter 6219, and a reflection. a piece 6225, a second collimating lens 6226, and a set of long pass filters 6227 disposed on the inner wall of the light source device 621. The long pass filter 6227 is electrically connected to the spectrometer 622 (the long pass filter 6227 and Spectrometer 622 with fiber optic system The electrical connection is a conventional technique, so the detailed operation mode is not described. According to the optical focus control group 62, the Raman spectrum can be read and input into the electronic device 7 for qualitative and quantitative analysis. .

(4) Please refer to the first and seventh figures. In order to control the wavelength, power, light intensity and brightness of the light source 6211, for example, select the far-infrared light source 6211 that does not emit fluorescence emission to reduce the background fluorescence. The generation of the noise further includes a light focusing control device 623 electrically connected to the light source 6211, and the light source 6211 is provided with at least two light emitting patterns 62111, and the light focusing control device 623 regulates the light emitting of any of the light emitting crystals 62111. State and illuminating wavelength, in this embodiment, two illuminating crystals 62111 are respectively projected with two different wavelengths of light (for example, 784 nm and 785 nm, or 637 nm and 638 nm, or 531 nm and 532 nm), and the two are compared. Light is used to subtract the resulting background fluorescence noise.

(5) As shown in FIG. 16, the electronic device 7 includes a data storage unit 71, a receiving unit 72, and a processing unit 73. The data storage unit 71 and the receiving unit 72 and the processing unit 73 transmit data to each other. The data storage unit 71 stores a plurality of sample weight information, a plurality of sample Raman spectrum information, and a plurality of sample scattering intensity values, and according to the processing unit 73, the relative relationship between the weight information of the object to be identified, the Raman spectrum information, and the scattering intensity value, and the minimum Data analysis of the Least Square Method and the Full Constrained Least Square Method, comparing the weight information of the plurality of samples in the data storage unit 71, the Raman spectrum information of the complex sample, and the The baseline sample scattering intensity values are baseline corrected, the fluorescence spectra are subtracted, and the data are qualitatively and quantitatively analyzed for chemical species contained in the analyte.

(6) Referring to the seventh figure, the mobile platform 51 includes a plurality of displacement motors 511 that provide multi-directional displacement and rotation of the moving platform 51.

(7) Referring to the first figure, the sampling device 61 includes a plurality of moving motors 611 and a sampler 612. The complex moving motor 611 provides multi-directional displacement and rotation of the sampler 612, and the sampler 612 is a quantitative needle (Syringe a quantitative liquid pipette (Pipette) or a motorized automatic metering device (Motorized Auto-Pipette), the plurality of displacement motors 511 located on the moving platform 51 and the moving motor 611 located on the sampler 612 are controlled by the electronic device 7, Achieve full automation.

In addition, in the first embodiment of the present invention, referring to the fifteenth figure, the detecting portion 63 of the detecting slide 63 (the detecting slide 63 is a detecting wafer), the joint portion 81 of the detecting tube 8 of the second embodiment, and the first embodiment The third embodiment detects the joint portion 91 of the container 9 for chemical, physical, or raw a concentrated carrier for surface affinity or a surface-enhanced Raman-active substrate, the surface-enhanced Raman-active carrier is a sol of metal particles, such as silver, gold, copper, nickel, etc. a microscopic rough surface whereby the functional groups of the chemical molecules in the concentrate can be adsorbed by the active carrier, so that surface-enhanced Raman scattering spectroscopy (SERS) is used for micro molecular identification and spectral identification of the nano surface, so that even A small amount of molecular weight can also be used for high sensitivity identification. The surface of the joint portion 631 of the first embodiment is provided with a Raman spectral focusing spot 633, which is a cymbal or other Raman spectroscopy standard for the application of the concentrating focus setting. And a concentration limiting ring 632 (Retaining Ring) is disposed on the outer periphery of the joint portion 631. The material of the concentration limiting ring 632 is a chemical, physical, and biological mutual exclusion of a paraffin ring, a Teflon or other complex concentrate. Or an affinity chemical material to limit the concentration of concentrated droplets to the detection slide 63 and to maintain uniformity and reliability of the concentrate distribution. The illuminating source 6211 projects a ray scanning Raman spectroscopy focusing spot 633 having a wavelength of 520 nm to achieve the highest intensity automatically setting the focus of the light source to the detecting carrier 63.

According to the first embodiment of the above-mentioned intelligent automatic chemical substance qualitative and quantitative detection device and further technical features, the description of the qualitative and quantitative detection methods of the intelligent automatic chemical substance will be continued, please refer to the twelfth figure and together with the first to As shown in Fig. 8, (1) Sample positioning: the container 1 to be tested equipped with the object to be tested is put into the extraction device 3, and the object to be tested is placed on the weight measuring unit 35, and the weight measuring unit 35 generates weight information, and then operates the switch. The member 34 clamps the perforation group 33 and partially penetrates into the container 1 to be tested while closing the placement portion 31; (2) Solvent treatment: the solvent in the solvent treatment device 2 (the solvent is an organic solvent, an aqueous solvent, a mixed solvent) Or carbon dioxide) is heated and pressurized and sent to the extraction device 3, the detailed process of which is to activate the solenoid valve 23 located in the front line 41 to allow the solvent to flow from the reservoir bottle 21 to the heating chamber 22 to be heated to a set temperature, and then close the solenoid valve 23 . (3) Extraction: the solvent flows into the container 1 to be tested and mixed with the analyte to produce an extract, and the extraction pipette 36 drops the collected extract into the collection container 52, and the detailed process adjusts the moving platform 51 to make the collection container. 52 is aligned with the titration end 361 of the extraction dropper 36, activates the solenoid valve 24 located in the rear line 42, and activates the pressurized pump 25 to deliver the heated solvent to the detection channel 32, and flows from the needle piece 331 to the test. The inside of the container 1 is mixed with the analyte to produce an extract, and the collected extract is dropped into the collection container 52 by the extraction dropper 36 (the pressure pump 25 generates a pressure in the range of 0.1 kg/cm ² to 1000 kg/cm ² ) . (4) Concentration: heating and blowing to accelerate the evaporation of the extract in the collecting container 52 to obtain a concentrated liquid; (5) Analysis: the sampling device 61 sucks the concentrated liquid, and drops the concentrated liquid to the detecting slide 63 and the joint portion 631. After the combined air blow drying, the Raman spectrum signal is read by the light focus control group 62, and input to the electronic device 7 for analysis, and the electronic device 7 analyzes the Raman spectrum signal and the weight information to analyze the object to be detected. The chemical substance contained in the process is such that the displacement motor 511 displaces the moving platform 51 below the sampler 612, and the sampler 612 places the end of the sampler 612 into the collection container 52 by moving the motor 611 and moves the motor 611 to suck The concentrate (e.g., 0.5 microliter) is metered, and then the displacement motor 511 aligns the test slide 63 with the sampler 612, and the absorbed concentrate is dropped onto the joint 631 of the test slide 63, which is quickly dried after the sample is processed. The displacement motor 511 displaces the moving platform 51 to the light focusing control group 62, and the Raman spectrum is read by the projected light (laser laser) and the spectrometer 622 (Raman spectroscopy), and is input to the electronic device 7 for reading and pulling. Manguang The Raman spectrum was compared.

Among them, the intelligent automatic chemical substance qualitative and quantitative detection method An embodiment includes the following further technical features:

(1) In the analyzing step, the electronic device 7 compares the Raman spectral signal and the weight information of the object to be detected with the Raman spectrum signal of the plurality of samples stored in the electronic device 7, and the weight information of the plurality of samples, the baseline correction, and the firefly. Subtraction of light spectrum, data qualitative and quantitative analysis, The data contained in the object to be detected is analyzed using the data of the Least Square Method and the Full Constrained Least Square Method. The substance is used to provide a fully automated and simple operation to quickly detect, characterize, and quantify the results of the test in the side container 1.

(2) In order to reduce noise, the light focusing control group 62 emits a first light and a second light. The first light and the second light have relatively close wavelengths, and the first spectrum is generated by scanning and detecting the object to be detected. The second spectrum is used to obtain the qualitative and quantitative results of detecting the dark sample by subtraction and baseline correction of the fluorescence spectrum, or the light focus control group 62 emits a far infrared light to scan and detect the object to be detected. Qualitative and quantitative results of detectable dark samples without Raman spectrograms of fluorescent noise, such as qualitative and quantitative results for red wine (regardless of the first or second light path and the thirteenth The figures are the same, so they are not shown in other drawings and are not repeated.)

Please refer to the seventeenth figure, which is a method for detecting melamine (chemical formula: C ₃ H ₆ N ₆ ) at a laser wavelength of 785 nm, and the chemical structural formula is as follows:

In the seventeenth figure, the vertical axis coordinates are the intensity (Intensity (counts/s)), the horizontal axis coordinates are the wave number (Wavenumber (cm ^-1 )), and the melamine is 378.7 cm ^-1 , 387.6 cm ^-1 , 581.5 cm ^{-1 .} 675.9cm ^-1 , 774.7cm ^-1 , 986.2cm ^-1 , 1445.3cm ^-1 , 1560.2cm ^{-1 are} stronger, and the highest peak is at 675.9cm ^-1 .

Referring to FIG. 18, the sensitivity of the melamine on the detection carrier 63 (SERS) substrate is measured at a laser wavelength of 785 nm, and the obtained Raman shift spectrum is in the range of 300 cm ^-1 to 1800 cm ^-1 . In the figure, A to F are different concentrations of melamine, A concentration is 10 ^-2 molL ^-1 , B concentration is 10 ^-3 molL ^-1 , C concentration is 10 ^-4 molL ^-1 , D concentration is 10 ^-5 molL ^-1 , E concentration is 10 ^-6 molL ^-1 , F concentration is 10 ^-7 molL ^-1 , G is SERS substrate gold substance (gold subtrate), as can be seen from the twentieth figure, the invention E is still The peak of 676 cm ^-1 can be clearly detected, so the sensitivity of the present invention can reach a concentration of ^10-6 molL ^-1 of melamine.

Please refer to the twentieth figure, which is a Raman spectrum obtained by adjusting the focusing distance of 10 ^-4 M melamine on the detection slide 63 (SERS substrate) at 675.9 nm (melamine) and at 520 nm (Si). The best Raman spectral intensity is achieved when the focusing distance is adjusted from C to B to A, and A is the optimum focusing distance.

The invention further describes a second embodiment of the qualitative and quantitative detection method of the intelligent automatic chemical substance according to the above-mentioned intelligent automatic chemical substance qualitative and quantitative detection device, as shown in the twentieth figure, together with the ninth As shown in FIG. 10, it comprises the following steps: (1) Sample positioning: the container 1 to be tested containing the object to be tested is put into the extraction device 3, and then the switch member 34 is operated to clamp the perforation group 33 to the container 1 to be tested. Passing into the container 1 to be tested; (2) Solvent treatment: heating and pressurizing the solvent in the solvent treatment device 2 to the extraction device 3; (3) Extraction: the solvent flows into the container 1 to be tested and mixed with the analyte to produce an extract; (4) Analysis: the extract is sent from the outlet end 37 to the test tube 8, and the molecules and the joints in the extract 81 After combining, heating and blowing drying, a Raman spectral signal is read by the light focusing control group 62 and input to the electronic device 7 for analysis, and the electronic device 7 compares the Raman spectral signal and the weight information to analyze the object to be detected. Chemical substances contained. (The step flow of the second embodiment of the present invention differs from the step flow of the first embodiment in that the second embodiment does not require a concentration step, but directly extracts the extract from the extraction device 3 into the test tube 8, and other processes The detailed process is the same as that of the first embodiment, so the description will not be repeated.

The second embodiment of the method for detecting qualitative and quantitative intelligent chemical substances of the present invention further includes the following technical features: (1) In the analyzing step, the electronic device 7 stores and stores the Raman spectral signal and weight information of the object to be detected. Comparison of Raman Spectral Signals and Complex Sample Weight Information in Multiple Samples, Baseline Correction, Fluorescence Spectral Subtraction, Data Qualitative and Quantitative Analysis, Applying Least Square Method and Fully Constrained Least Square Error The data of the Full Constrained Least Square Method analyzes the chemicals contained in the analyte. (2) the light focusing control group 62 emits the first light and the second light, wherein the first light and the second light have similar wavelengths, and when the object to be detected is scanned and scanned, the first spectrum and the second spectrum are generated, thereby generating a first spectrum and a second spectrum. The subtraction of the fluorescence spectrum and the baseline correction are used to obtain qualitative and quantitative results of detecting the dark sample, or the light focus control group 62 emits a far-infrared light, thereby scanning and detecting the object to be detected, thereby generating a mixture containing no fluorescence. The Raman spectrum of the signal is used to achieve qualitative and quantitative results for detectable dark samples. (3) In the sample positioning step, the weight measuring unit 35 measures the weight data of the object to be tested into the electronic device 7. (4) In the solvent treatment step, the second valve 441 is opened to transfer the solvent in the liquid storage bottle 21 to the heating chamber 22 for heating. (5) The solvent is an organic solvent, water, a mixed solvent or carbon dioxide. (6) In the solvent treatment step, the gas sent from the pressurizing pump 25 has a gas pressure of 0.1 kg/cm ² to 1000 kg/cm ² . (7) In the extraction step, the first valve 431 is opened to allow the gas in the pressurizing pump 25 to flow into the detecting passage 32, and the solvent is pushed into the container 1 to be tested, and finally, the detecting tube 8 is combined with the joint portion 81.

The second embodiment of the qualitative and quantitative detection method of the intelligent automatic chemical substance of the present invention is the same as the detailed process steps in the first embodiment, and therefore is not repeated. Description.

The invention further describes a third embodiment of the qualitative and quantitative detection method of the intelligent automatic chemical substance according to the above-mentioned intelligent automatic chemical substance qualitative and quantitative detection device, please refer to the sixth figure and together with the eleventh and the twelfth figure. As shown, the steps include: sample positioning: the container 1 to be tested equipped with the object to be tested is put into the extraction device 3, and the object to be tested is placed on the weight measuring unit 35, and the weight measuring unit 35 generates weight information, and then the switch member 34 is operated. The perforated group 33 is clamped and partially penetrated into the container 1 to be tested; solvent treatment: the solvent in the solvent treatment device 2 is heated and pressurized and sent to the extraction device 3 (which pushes the heated solvent by the pressurizing pump 25) The solvent treatment device 2 to the extraction device 3, the pressure pump 25 generates a pressure in the range of 0.1 kg/cm ² to 1000 kg/cm ² ; extraction: the solvent flows into the container 1 to be tested and mixed with the analyte to produce an extract And extracting the dropper 36 to drop the collected extract into the detecting container 9 (the organic solvent, the water solvent or the mixed solvent in the embodiment); concentrating: heating and blowing the extract in the detecting container 9 to evaporate the solvent A concentrated liquid; analysis: after the light focusing control group 62 detects the bonding portion 91 of the container 9, the Raman spectrum signal is read and input to the electronic device 7 for analysis, and the electronic device 7 analyzes the Raman spectral signal and weight. Information to analyze the chemicals contained in the analyte.

The invention discloses a qualitative and quantitative detection method for intelligent automatic chemical substances of the invention The third embodiment further includes the following technical features: (1) after the concentrated liquid is combined with the joint portion 91 in the detecting container 9, the light focusing control group 62 is directly irradiated to obtain a Raman spectrum, and the Raman spectrum is transmitted to The electronic device 7 is aligned. (2) In addition, in the analyzing step, the electronic device 7 compares the Raman spectral signal and weight information of the object to be detected with the complex sample Raman spectral signal and the plurality of sample weight information stored in the electronic device 7, and the baseline Calibration, fluorescence spectral subtraction, data qualitative and quantitative analysis, application of the Least Square Method and Full Constrained Least Square Method data analysis of the chemistry of the analyte The substance (the effect achieved is the same as that of the first and second embodiments, so the description will not be repeated).

The above is only a preferred embodiment for explaining the present invention, and is not intended to limit the invention in any way, so that any modifications relating to the present invention are made in the spirit of the same invention. And the changer, other types that can be implemented and have the same effect, should still be included in the scope of the intention of the present invention.

In summary, the "intelligent automatic chemical substance qualitative and quantitative detection device and detection method" of the present invention is practically and cost-effectively required to fully meet the needs of industrial development, and the disclosed structural invention is also It has an unprecedented innovative structure, so its "novelty" should be undoubtedly considered, and the invention can be more effective than the conventional structure, so it is also "progressive", which fully complies with the application for the invention patent of the Chinese Patent Law. The requirements of the requirements are to file a patent application in accordance with the law, and I would like to ask the bureau to review it as soon as possible and give it affirmation.

1‧‧‧ Container to be tested

2‧‧‧Solvent treatment unit

21‧‧‧Liquid bottle

22‧‧‧heating room

23‧‧‧ solenoid valve

24‧‧‧ solenoid valve

25‧‧‧Pressure pump

3‧‧‧ Extraction device

31‧‧‧Placement Department

32‧‧‧Detection channel

33‧‧‧Perforation group

34‧‧‧Switches

35‧‧‧ Weight measuring unit

36‧‧‧ Extraction dropper

361‧‧‧Trip end

38‧‧‧Extraction machine

4‧‧‧pipe

41‧‧‧ Front pipeline

42‧‧‧After the pipeline

51‧‧‧Mobile platform

511‧‧‧displacement motor

52‧‧‧Collection container

6‧‧‧Detection unit

61‧‧‧Sampling device

611‧‧‧Moving motor

612‧‧‧sampler

62‧‧‧Light Focus Control Group

621‧‧‧Light source device

622‧‧‧ Spectrometer

623‧‧‧Light focus control device

63‧‧‧Test slides

7‧‧‧Electronic devices

Claims (41)

The invention relates to a qualitative and quantitative detection device for intelligent automatic chemical substances, which comprises: a container to be tested for storing the object to be detected; a solvent treatment device for storing, heating and pressurizing the solvent; and an extraction device; The utility model comprises an extraction machine, a perforation group, a switch component, a weight measuring unit and an extraction dropper, wherein the extraction machine is provided with a placing portion, and a detecting channel connected with the placing portion is arranged in the extracting machine The perforation group activity group is disposed in the detection channel, and the switch member is arranged in linkage with the perforation group, the weight measurement unit is corresponding to the placement portion and is disposed in the detection channel, and the extraction dropper group is disposed at the end of the extraction machine, and is detected The channel is connected, the extraction pipette is provided with a titration end, the solvent is mixed with the object to be detected through the extraction machine to form an extract, and the weight measuring unit outputs a weight information; a pipeline connecting the solvent treatment device and the extraction device a detecting device; a concentrating device comprising a moving platform and a collecting container, the moving platform is provided with a heating portion, the collecting container is placed in the heating portion, and is heated Extracting a pipette to condense the extract into a concentrated liquid; a detecting unit comprising an adjusted sampler, a light focusing control group and a detecting carrier, the detecting carrier set on the mobile platform, and The detecting end surface of the detecting piece is provided with a joint portion which is combined with the molecules in the concentrated liquid, and the light focusing control group reads the light and then reads a Raman spectrum signal; and an electronic device electrically connected to the solvent processing device and the extraction device a concentrating device, a detecting unit, and an electronic device receiving the Raman spectrum signal to analyze the chemical substances contained in the detecting object, At the same time, the electronic device receives the weight information; accordingly, after the molecules of the concentrated liquid are combined with the detecting slide, the weight information provided by the Raman spectral signal and the weight measuring unit is read by the optical focus control group to the electronic device, and the electronic device performs the After the weight information of the analyte and the Raman spectral signal are analyzed, the chemical substance of the object to be detected is recombined, thereby achieving the effect of qualitatively and quantitatively detecting the object to be detected, providing a quick and easy operation, fully automated and highly sensitive detection. The function allows the user to quickly detect the chemical substances contained in the object to be detected, and achieve qualitative and quantitative effects of quality control and testing. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 1, wherein the solvent treatment device comprises a liquid storage bottle for storing a solvent, a heating chamber for heating the solvent, two electromagnetic valves, and a a heating pump connected to the heating chamber, and the pipeline includes a front pipeline and a rear pipeline, the front pipeline communicates with the liquid storage bottle and the heating chamber, and the pipeline is connected to the heating chamber and the detection channel, and the two solenoid valves respectively The group is arranged on the front pipeline and the rear pipeline, and the two solenoid valves adjust the liquid storage bottle and the heating chamber, and the heating chamber and the detection passage are in a conducting state or a blocking state, and the pressurized pump is used as a pushing solvent. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 1, wherein the perforating group comprises two oppositely disposed needle segments, and the shape of each needle segment corresponds to the structure of the container to be tested, and each The end surface of the needle piece has a plurality of needle tubes. When the two needle pieces are clamped to the two sides of the container to be tested, the plurality of needle tubes of each needle piece will penetrate into the container to be tested. The invention relates to a qualitative and quantitative detection device for intelligent automated chemical substances according to claim 1, wherein the detecting portion of the detecting slide is a surface-enhanced Raman-active substrate (SERS-active substrate), and the surface-enhanced Raman The active carrier is a sol of metal particles, and a Raman spectrum focusing spot is provided on the surface of the joint, and a concentrated liquid limit is arranged on the outer periphery of the joint. Retaining Ring, the concentrate restriction ring is made of paraffin ring or Teflon. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 4, wherein the light focus control group comprises a light source device and a spectrometer, and the light source device can project laser light and ultraviolet light (UV). a near-infrared (NIR), far infrared (FIR) or visible light, the light source device includes a body, an illumination source, an optical head, and a set of focal length adjusting mechanisms disposed on the optical head. The light source is disposed at one end of the body, the light source projects at least one wavelength of light, and generates at least one projection light path and at least one return light path, and the light head is disposed at the other end of the body, and the light head corresponds to the detection load. a film; the at least one projection optical path sequentially passes through a first collimating lens, a laser line filter, a color filter, a beam splitter, and finally passes through the optical head to the joint of the detecting carrier, and the projection optical path is divided into The beam generator generates a first focusing optical path through the beam splitter to the detecting carrier, and a second focusing optical path reflected by the beam splitter, the second focusing optical path is projected through a second focusing mirror to a set of a photosensitive element of the body; the bonding portion of the at least one return optical path detecting carrier sequentially passes through the optical head, the color filter, a reflective sheet, a second collimating lens and a set of long filtering filters disposed on the inner wall of the body The optical device is electrically connected to the spectrometer; the focal length adjusting mechanism, the photosensitive element and the spectrometer are electrically connected to the electronic device; and the electronic device compares the path length of the first focusing optical path with the second focusing optical path The difference in path length is automatically adjusted by the focal length adjustment mechanism to adjust the focal length of the optical head to adjust the focal length of the optical head and the detecting slide. The intelligent automatic chemical substance qualitative and quantitative detecting device according to claim 5, further comprising a light focusing control device electrically connected to the light source, and emitting The source is provided with at least two illuminating crystal grains, and the light focusing control device controls the illuminating state and the illuminating wavelength of any illuminating crystal grain, so that the illuminating source projects the light of the at least one wavelength, and controls the wavelength, power and light intensity of the illuminating crystal grain. And shiny state. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 1, wherein the electronic device comprises a data storage unit, a receiving unit and a processing unit, the data storage unit and the receiving unit and the processing unit are mutually Data transmission, the data storage unit stores the weight information of the plurality of samples, the Raman spectrum information of the plurality of samples, and the scattering intensity values of the plurality of samples, according to which the processing unit uses the weight information of the object to be identified, the Raman spectrum information, and the relative relationship of the scattering intensity values, Data analysis of the Least Square Method and the Full Constrained Least Square Method, comparing the weight information of the plurality of samples in the data storage unit, the Raman spectrum information of the complex sample, and the The baseline sample scattering intensity values are baseline corrected, the fluorescence spectra are subtracted, and the data are qualitatively and quantitatively analyzed for chemical species contained in the analyte. The intelligent automatic chemical substance qualitative and quantitative detecting device according to claim 1, wherein the moving platform comprises a plurality of displacement motors, and the complex displacement motor provides multi-directional displacement and rotation of the moving platform. The intelligent automatic chemical substance qualitative and quantitative detecting device according to claim 1, wherein the adjustable sampler comprises a plurality of moving motors and a sampler, wherein the plurality of moving motors provide a multi-directional displacement of the mobile sampler And rotating, and the sampler is a Syringe, a Pipette or a Motorized Auto-Pipette. A method for qualitative and quantitative detection of intelligent automated chemical substances, including Column step: sample positioning: the container to be tested equipped with the object to be tested is put into the extraction device, and the object to be tested is placed on the weight measuring unit, and the weight measuring unit generates the weight information, and then the switch member is operated to clamp the piercing group to the side. Passing into the container to be tested; solvent treatment: heating and feeding the solvent in the solvent treatment device to the extraction device; extracting: the solvent flows into the container to be tested and mixed with the analyte to produce an extract, and the extraction pipette will collect The extracting liquid is dropped into the collecting container; the concentration: heating and blowing to accelerate the evaporation of the solvent in the collecting container to obtain a concentrated liquid; analysis: the adjusting sampler sucks the concentrated liquid, and drops to the detecting slide to combine with the joint to blow dry After that, the Raman spectrum signal is read by the light focus control group and input to the electronic device for analysis, and the electronic device analyzes the Raman spectrum signal and the weight information to analyze the chemical substances contained in the object to be detected. According to the qualitative and quantitative detection method of the intelligent automatic chemical substance described in claim 10, in the analysis step, the electronic device compares the Raman spectrum signal of the object to be detected, the weight information, and the plurality of samples stored in the electronic device. Raman spectral signal, complex sample weight information for comparison, baseline correction, fluorescence spectral subtraction, data qualitative and quantitative analysis, application of least square error method (Least Square Method) and fully constrained least square error method (Full Constrained The data of Least Square Method) analyzes the chemicals contained in the analyte. According to the qualitative and quantitative detection method of the intelligent automatic chemical substance described in claim 10, the light focus control group emits the first light and the second light, and the wavelengths of the first light and the second light are relatively close, thereby When scanning and detecting the object to be detected, the first spectrum image and the second spectrum image are generated, thereby performing the subtraction of the fluorescence spectrum and the baseline correction to obtain the qualitative property of detecting the dark sample. And the quantitative result, or the light focus control group emits a far-infrared light, thereby scanning and detecting the object to be detected, and generating a Raman spectrum of the noise without fluorescence to achieve qualitative and quantitative results of the detectable dark sample. The method for qualitative and quantitative detection of intelligent automated chemical substances according to claim 10, wherein in the solvent treatment step, the heated solvent is pushed from the solvent treatment device to the extraction device by a pressurized pump. The pressurizing pump generates a pressure in the range of 0.1 kg/cm ² to 1000 kg/cm ² . The method for qualitative and quantitative detection of intelligent automated chemical substances according to claim 10, wherein the solvent is an organic solvent, an aqueous solvent, a mixed solvent or carbon dioxide. The invention relates to a qualitative and quantitative detection device for intelligent automatic chemical substances, which comprises: a container to be tested for storing the object to be tested; a solvent treatment device for storing and heating the pressurized solvent; and an extraction device comprising An extraction machine, a perforation group, a switch member and a weight measuring unit, the extracting machine is provided with a placing portion, and the extracting machine is provided with a detecting channel communicating with the placing portion and a connecting channel with the detecting side channel The outlet end, the perforation group active group is disposed in the detection channel, and the perforation group clamps against and pierces the container to be tested, the switch member is pivotally disposed on the extraction machine, and is disposed in conjunction with the perforation group, and the weight measurement unit is correspondingly disposed. And the group is disposed in the detection channel, and the outlet end is connected to the detection channel, and the heated solvent is introduced into the detection channel by the pipeline, and the object to be detected in the container to be tested is generated through the perforation group to generate an extract, and the weight measuring unit is Outputting a weight information; a pipeline connecting the solvent treatment device and the detection channel of the extraction device; a detection tube set at the outlet end and connected to the detection channel And detection The inner ring of the test tube is provided with a joint portion, and the extract liquid is connected to the test tube to be combined with the joint portion; a detecting unit includes a light focus control group, and the light focus control group reads a Raman spectrum signal after projecting the light to the joint portion. An electronic device electrically connected to the solvent processing device, the extraction device, and the detecting unit, the electronic device receiving the Raman spectral signal, and analyzing the Raman spectral signal to obtain information of the chemical substance contained in the detecting object, and the electronic device Receiving the weight information; accordingly, obtaining the concentrated liquid by the solvent processing device and the extraction device, and combining the molecules of the concentrated liquid with the detection tube, and reading the Raman spectral signal and the weight measuring unit through the light focusing control group The weight information is provided to the electronic device, and the electronic device compares and analyzes the weight information of the object to be detected and the Raman spectrum signal, and then recombines the chemical substances of the object to be detected to achieve qualitative and quantitative effects of the object to be detected. Provides fast and easy operation, fully automated and highly sensitive detection, allowing users to quickly detect It contains chemical substances and to achieve quality control and efficacy testing. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 15 , wherein the solvent treatment device comprises a pressure pump, a liquid storage bottle, and a heating connection with the liquid storage bottle and the detection channel. a pipeline, the pipeline includes a first pipeline and a second pipeline, the first pipeline is connected to the pressure pump and the detection channel, and the second pipeline is connected to the liquid storage bottle and the detection channel, and is connected to the first pipeline A first valve is provided and a second valve is disposed on the second line. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 15 , wherein the perforation group comprises two oppositely disposed needle segments, and the shape of each needle segment corresponds to the structure of the container to be tested, and each The end surface of the needle piece has a plurality of needle tubes. When the two needle pieces are clamped to the two sides of the container to be tested, the plurality of needle tubes of each needle piece will penetrate into the container to be tested. The invention relates to a qualitative and quantitative detection device for intelligent automated chemical substances according to claim 15, wherein the joint of the test tube is a surface-enhanced Raman-active substrate and surface-enhanced Raman activity. The carrier is a sol of metal particles. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 18, wherein the light focus control group comprises a light source device and a spectrometer, and the light source device can project laser light and ultraviolet light (UV). a near-infrared (NIR), far infrared (FIR) or visible light, the light source device includes a body, an illumination source, an optical head, and a set of focal length adjusting mechanisms disposed on the optical head. The light source is disposed at one end of the body, the light source projects at least one wavelength of light, and generates at least one projection light path and at least one return light path, and the light head is disposed at the other end of the body, and the light head corresponds to the detection tube The at least one projection light path is sequentially passed through a first collimating lens, a laser line filter, a color filter, a beam splitter, and finally through the junction of the optical head to the detecting tube, and the at least one projection The optical path generates a first focusing optical path through the beam splitter to the detecting portion of the detecting tube, and a second focusing optical path reflected by the beam splitter, the second focusing optical path is projected Passing a second focusing mirror to a group of photosensitive elements disposed on the body; the bonding portion of the at least one return optical routing detecting carrier sequentially passes through the optical head, the color filter, a reflective sheet, a second collimating lens, and a long pass filter disposed on the inner wall of the body, the long pass filter is electrically connected to the spectrometer; the focal length adjusting mechanism, the photosensitive element and the spectrometer are electrically connected to the electronic device; the electronic device is firstly matched The difference between the path length of the focusing optical path and the path length of the second focusing optical path is automatically adjusted by the focal length adjusting mechanism to adjust the focal length of the optical head to adjust the focal length of the optical head and the detecting tube. The apparatus for detecting qualitative and quantitative intelligent chemical substances according to claim 19, further comprising a light focusing control device electrically connected to the light source, wherein the light source is provided with at least two light emitting grains. The light focusing control device controls the light emitting state and the light emitting wavelength of any of the light emitting crystal grains, and causes the light emitting source to project the light of the at least one wavelength, and controls the wavelength, power, light intensity and lightening state of the light emitting crystal grain. The intelligent automatic chemical substance qualitative and quantitative detecting device according to claim 15 , wherein the light focusing control group is provided with a rotating motor, and the rotating motor drives the light source device to displace. A method for qualitative and quantitative detection of intelligent automated chemical substances, comprising the following steps: sample positioning: putting a container to be tested containing the object to be tested into an extraction device, and then operating the switch member to clamp the piercing group against the container to be tested and partially Penetrating into the container to be tested; solvent treatment: heating and pressurizing the solvent in the solvent treatment device to the extraction device; extracting: the solvent flows into the container to be tested and mixed with the analyte to produce an extract; analysis: the extract is exported The end is sent to the test tube, and the molecules in the extract are combined with the joint, heated and blown dry, and a Raman spectrum signal is read by the light focus control group, and input into the electronic device for analysis, and the electronic device is pulled. Mann spectrum signal and weight information to analyze the chemical substances contained in the analyte. According to the qualitative and quantitative detection method of the intelligent automatic chemical substance described in claim 22, in the analysis step, the electronic device compares the Raman spectral signal of the object to be detected, the weight information and the plurality of samples stored in Raman. Spectral signal, complex sample weight information for comparison, baseline correction, fluorescence spectral subtraction, data qualitative and quantitative analysis, application of least square error The data of the Least Square Method and the Full Constrained Least Square Method analyzes the chemical substances contained in the analyte. According to the qualitative and quantitative detection method of the intelligent automatic chemical substance described in claim 22, the light focus control group emits the first light and the second light, and the first light and the second light have similar wavelengths, thereby scanning When detecting the object to be detected, a first spectrum image and a second spectrum image are generated, by which the fluorescence spectrum is subtracted and the baseline is corrected to obtain qualitative and quantitative results of the dark fluorescent radiation sample, or the light focus control group is irradiated. A far-infrared light, by scanning to detect the object to be detected, produces a Raman spectrum of no-noise noise to achieve qualitative and quantitative results of the detectable dark sample. The method for detecting qualitative and quantitative intelligent chemical substances according to the scope of claim 22, wherein in the sample positioning step, the weight side unit measures the weight data of the test object into the electronic device. The method of operating an automated detection, characterization, and quantification apparatus and method of claim 22, wherein in the solvent treatment step, a second valve is opened to transfer the solvent in a reservoir to the heating chamber for heating. The method for qualitative and quantitative detection of intelligent automated chemical substances according to claim 22, wherein the solvent is an organic solvent, water, a mixed solvent or carbon dioxide. According to the method for detecting qualitative and quantitative intelligent chemical substances according to claim 22, in the extraction step, the first valve is opened to allow the gas in the pressure pump to flow into the detection channel, and the solvent is pushed to be treated. Measure the inside of the container and finally into the test tube. According to the method for detecting qualitative and quantitative intelligent chemical substances according to the scope of claim 28, in the solvent treatment step, the gas sent by the pressurized pump has a gas pressure of 0.1 kg/cm ² to 1000 kg/cm ^{2 .} . The invention relates to a qualitative and quantitative detection device for intelligent automatic chemical substances, which comprises: a container to be tested for storing the object to be detected; a solvent treatment device for storing, heating and pressurizing the solvent; and an extraction device; The utility model comprises an extraction machine, a perforation group, a switch component, a weight measuring unit and an extraction dropper, wherein the extraction machine is provided with a placing portion, and a detecting channel communicating with the placing portion is arranged therein, the perforating The group active group is disposed in the detecting channel, and the switch component is arranged in linkage with the punching group, the weight measuring unit is corresponding to the placing portion and is disposed in the detecting channel, and the extracting dropper group is disposed at the end of the extracting machine and communicating with the detecting channel, The extraction pipette is provided with a drop end, the solvent is mixed with the object to be detected by the extraction device to form an extract, and the weight measuring unit outputs a weight information; a pipeline connecting the solvent treatment device and the detection channel of the extraction device; The device comprises a moving platform and a detecting container, wherein the moving platform is provided with a heating portion, the detecting container is placed on the heating portion, and the detecting container has a The extracting liquid is in the detecting container, and is concentrated into a concentrated liquid by heating and blowing, and the molecules in the concentrated liquid are combined with the joint; a detecting unit includes a light focusing control group, and the light focusing control group includes a a light source device, the light source device is provided with an optical head at one end, the optical head passes through the moving platform and corresponds to the joint portion of the detecting container, and the light focusing control group projects the light to the joint portion to read a Raman spectrum signal; an electronic device, The electrical connection solvent processing device, the extraction device, the concentrating device, the detecting unit, the electronic device receives the chemical substance contained in the Raman spectrum signal, and the electronic device receives the weight information. According to the intelligent automatic chemical substance qualitative and quantitative detecting device described in claim 30, the solvent processing device comprises a liquid storage bottle for storing a solvent, a heating chamber for heating the solvent, two electromagnetic valves, and a heating chamber. a connected pressure pump, and the pipeline includes a front pipeline and a rear pipeline, the front pipeline communicates with the liquid storage bottle and the heating chamber, and the pipeline is connected to the heating chamber and the detection channel, and the two solenoid valves are respectively set up On the front pipeline and the rear pipeline, the two solenoid valves adjust the liquid storage bottle and the heating chamber, the heating chamber and the detection passage to be in a conducting state or a blocking state, and the pressure pump functions as a pressurizing and pushing solvent. According to claim 30, the intelligent automatic chemical substance qualitative and quantitative detecting device according to claim 30, wherein the perforating group comprises two oppositely disposed needle segments, and the shape of each pin segment corresponds to the structure of the container to be tested, and each The end surface of the needle piece has a plurality of needle tubes. When the two needle pieces are clamped to the two sides of the container to be tested, the plurality of needle tubes of each needle piece will penetrate into the container to be tested. The invention relates to a qualitative and quantitative detection device for intelligent automated chemical substances according to claim 30, wherein the joint of the detection container is a surface-enhanced Raman-active substrate (SERS-active substrate), and the surface-enhanced Raman-active carrier It is a sol body of metal particles. The intelligent automatic chemical substance qualitative and quantitative detecting device according to claim 30, wherein the light focusing control group comprises a light source device and a spectrometer, and the light source device can project laser light and ultraviolet light (UV). a near-infrared (NIR), far infrared (FIR) or visible light, the light source device includes a body, an illumination source, an optical head, and a set of focal length adjusting mechanisms disposed on the optical head. The light source is disposed at one end of the body, the light source projects at least one wavelength of light, and generates at least one projection light path and at least one return light path, and the light head is disposed at the other end of the body, and the light head corresponds to the detection load. a sheet; the at least one projection light path sequentially passes through a first collimating lens, a laser line filter, and a color The filter and a beam splitter finally pass through the junction of the optical head to the detecting carrier, and the projection optical path generates a first focusing optical path through the beam splitter to the detecting carrier, and is split. Reflecting a second focusing optical path, the second focusing optical path is projected through a second focusing mirror to a group of photosensitive elements disposed on the body; the bonding portion of the at least one return optical routing detecting carrier sequentially passes through the optical head, the color filter a light sheet, a reflection sheet, a second collimating lens and a set of long-pass filters disposed on the inner wall of the body, the long-pass filter being electrically connected to the spectrometer; the focal length adjusting mechanism, the photosensitive element and the spectrometer Electrically connecting with the electronic device; the electronic device compares the path length of the first focusing optical path with the path length of the second focusing optical path, and automatically adjusts the focal length of the optical head by the focal length adjusting mechanism to adjust the focal length of the optical head and the detecting container . The intelligent automatic chemical substance qualitative and quantitative detecting device according to claim 34, further comprising a light focusing control device optical focusing control device electrically connected to the light source, wherein the light source is provided with at least two light crystals The light focusing control device controls the light emitting state and the light emitting wavelength of any of the light emitting crystal grains, and causes the light emitting source to project the light of the at least one wavelength, and controls the wavelength, power, light intensity and lightening state of the light emitting crystal grain. For example, the intelligent automatic chemical substance qualitative and quantitative detecting device described in claim 30, the electronic device includes a data storage unit, a receiving unit and a processing unit, the data storage unit and the receiving unit and the processing unit are mutually Data transmission, the data storage unit stores the weight information of the plurality of samples, the Raman spectrum information of the plurality of samples, and the scattering intensity values of the plurality of samples, according to which the processing unit uses the weight information of the object to be identified, the Raman spectrum information, and the relative relationship of the scattering intensity values, Least Square Method and Least Square Method Data analysis of the Full Constrained Least Square Method, comparing the weight information of the plurality of samples in the data storage unit, the Raman spectrum information of the complex sample, and the scattering intensity values of the complex sample for baseline correction and fluorescence spectrum phase Reduction, and data qualitative and quantitative analysis of the chemical substances contained in the test substance. The invention relates to a method for detecting qualitative and quantitative intelligent chemical substances, which comprises the following steps: sample positioning: the container to be tested equipped with the object to be tested is put into an extraction device, and the object to be tested is located on the weight measuring unit, and the weight measuring unit generates a weight information, and then operating the switch member to clamp the perforated group and partially penetrate the container to be tested; solvent treatment: heating and pressurizing the solvent in the solvent treatment device to the extraction device; and extracting: the solvent flows into the container to be tested Mixing with the analyte to produce an extract, and extracting the dropper to drop the collected extract into the detection container; concentrating: heating and blowing the detection solution in the container to volatilize the solvent to obtain a concentrated solution; analysis: light After the focus control group detects the junction of the container after the irradiation, the Raman spectrum signal is read and input to the electronic device for analysis, and the electronic device analyzes the Raman spectral signal and the weight information to analyze the chemical contained in the object to be detected. substance. According to the method for qualitative and quantitative detection of intelligent automated chemical substances described in claim 37, in the analysis step, the electronic device compares the Raman spectral signal of the object to be detected, the weight information and the plurality of samples stored in Raman. Spectral signal, complex sample weight information for comparison, baseline correction, fluorescence spectral subtraction, data qualitative and quantitative analysis, application of least square error method (Least Square Method) and fully constrained least square error method (Full Constrained The data of Least Square Method) analyzes the chemicals contained in the analyte. According to the method for qualitative and quantitative detection of intelligent automated chemical substances according to claim 37, the light focusing control group emits a first light and a second light, and the first light and the second light have similar wavelengths. By scanning to detect the object to be detected, a first spectrum image and a second spectrum image are generated, thereby performing qualitative and quantitative detection of the dark fluorescent radiation sample by subtracting and baseline correction of the fluorescence spectrum, or the light focusing control The group emits a far-infrared light, thereby scanning and detecting the object to be detected, and generating a Raman spectrum of the noise without fluorescence to achieve qualitative and quantitative results of the detectable dark sample. The method for qualitative and quantitative detection of intelligent automated chemical substances according to claim 37, wherein in the solvent treatment step, the pressurized solvent is used to push the heated solvent from the solvent treatment device to the extraction device. The pressure pump produces a pressure in the range of 0.1 kg/cm ² to 1000 kg/cm ² . The method for qualitative and quantitative detection of intelligent automated chemical substances according to claim 40, wherein the solvent is an organic solvent, an aqueous solvent, a mixed solvent or carbon dioxide.