TW201427674A - Phaseolus vulgaris seed extract and its preparation method and uses - Google Patents

Abstract

Phaseolus vulgaris seed extract is disclosed, which is prepared by the following steps: (a) grinding the Phaseolus vulgaris seeds to obtain Phaseolus vulgaris powder; (b) using a first solvent to dissolve the Phaseolus vulgaris powder to get a homogeneous Phaseolus vulgaris extract; (c) and centrifuging the homogeneous Phaseolus vulgaris extract and collecting Phaseolus vulgaris extract supernatant; wherein in the step b), roughly one gram of Phaseolus vulgaris powder is dissolved in 10 ml of first solvent for extraction.

Description

Flower bean seed extract, preparation method and use thereof

The present invention relates to a flower bean seed extract, and more particularly to a flower bean seed extract which can regulate blood sugar of a mammal, a preparation method and use thereof.

Flower bean seeds are extremely nutritious and rich in mineral elements (calcium, copper, iron, manganese, magnesium, zinc), proteins, vitamins and polyphenols. In addition to being used as vegetables, the fresh pods can be made into salted products and canned foods. The seeds can also be processed into bean fillings, cakes, soy sauce and monosodium glutamate. In addition, the whole plant can be used as feed. Because flower beans are rich in various functional ingredients, they are also considered to be health foods that are good for human health.

Historical literature shows that polyphenolic compounds will act in the digestive system and regulate the decomposition and absorption of carbohydrates in the human body. Therefore, there is a huge market demand, and related functional foods or health foods have been asked about the market, so how to enhance the existing flowers There is room for improvement in the technology for efficient extraction of high levels of polyphenolic compounds from other active ingredients.

In view of the above, the main object of the present invention is to provide a seed extract of Pondolus vulgaris L., which is obtained by the method comprising the steps of: a) grinding the flower bean seed to obtain a single bean powder; Dissolving the flower bean powder using a first solvent to obtain a homogenous soybean extract; and c) centrifuging the homogenous bean extract and collecting a supernatant bean extract, wherein the step b) is about One gram of the flower bean powder was dissolved in a ratio of 10 ml of the first solvent, and the first solvent was selected from the group consisting of water, C ₁ -C ₄ alcohols, and a combination thereof.

A second object of the present invention is to provide a method of preparing the above extract.

Another object of the present invention is to provide a food or drink for regulating blood sugar in a mammal comprising an effective amount of the above extract.

It is still another object of the present invention to provide a use of the above-described extract for the manufacture of a medicament for regulating blood sugar in a mammal.

A further object of the present invention is to provide a pharmaceutical composition for modulating a blood glucose cell of a mammal comprising an effective amount of the above extract.

A final object of the present invention is to provide a pharmaceutical composition for modulating a blood glucose cell of a mammal comprising an effective amount of the above extract and at least one saccharide metabolism related inhibitor.

In the present invention, the extract may be administered alone or in a composition containing the extract and a pharmaceutically acceptable carrier, diluent and/or excipient. The dosage administered can be adjusted depending on the health condition of the individual or the disease to be prevented or treated.

Unless otherwise defined herein, the scientific or technical terms of the invention used herein are intended to be understood by those of ordinary skill in the art. The meaning and scope of these terms should be clear, however, once there is any ambiguity, the definitions in this document take precedence over any dictionary or external definition.

The technical and preferred embodiments of the present invention will be described in the following, and the present invention will be understood by those of ordinary skill in the art to which the present invention pertains. To limit the scope of the invention.

Each of the specific embodiments of the present invention involved in the field yield test, component analysis, and enzyme inhibition activity analysis sample determination were three replicates, and statistical analysis was performed by statistical analysis SAS (statistics analysis syatem), and strains were compared using variant analysis (ANOVA). Differences between treatment and interim, and significant differences between strains or treatment combinations were performed by Fisher's least siginificant difference method (LSD), which was P<0.05.

[Example 1] Preparation method of flower bean seed extract containing high total polyphenol amount

I. Source of Bean Material

The plant material of the present invention is a tomato flower cultivar Hwachia and a sodium azide mutagenesis strain SA-05. The above-mentioned materials were planted at the Taiwan Agricultural Research Institute, and they were cultivated according to the management method of the cultivation of flower beans. They were planted in spring 2011, autumn 2011 and spring 2012. The dried pods were harvested for investigation of yield components, and the seeds were stored at 4 ° C for use.

II. Preparation process of flower bean seed extract

Please refer to the first figure. First, in step S11, the aforementioned bean seed is ground to obtain a single bean powder, and the preferred bean is selected from the group consisting of Hwachia. The mutagenesis line obtained by mutagenesis of the flower pods by sodium azide was selected. In this example, the mutagenesis was selected from the SA-05 line.

Subsequently, in step S12, the soybean powder was dissolved using the first solvent, and the treatment was carried out in the dark and shaking at 150 rpm for 1 hour. The first solvent is selected from the following group: water, C ₁ -C ₄ alcohols, and combinations of any one. Preferably, in the present embodiment, the first solvent is an aqueous methanol solution, and the aqueous methanol solution is used in an amount of from about 70% by volume to about 90% by volume. The best one is 80% methanol aqueous solution. In another embodiment, preferably the first solvent is an aqueous ethanol solution, and the aqueous ethanol solution is used in an amount of from about 40% by volume to about 60% by volume. The best one is a 50% aqueous solution of ethanol.

Next, in step S13, the homogenized soybean extract was centrifuged for 30 minutes, and then a supernatant of the supernatant was collected, and the centrifugation condition was 10,000 × g.

In step S14, the supernatant bean extract is filtered to obtain a purified flower bean extract, and the syringe filter used for filtration is purchased from Pall Corporation, and the membrane pore diameter used is used. It is 0.2 μm.

Finally, in step S15, the purified bean extract is stored at minus 20 degrees to obtain a purified bean seed extract containing a high total polyphenol amount.

The user can perform the freeze-vacuum drying treatment on the purified soybean bean seed extract to obtain a freeze-dried soybean extract, wherein the freeze-dried soybean extract can preserve several proteins.

III. Determination of Total Phenol, Total Flavonoids and Total Anthocyanin Content in Flower Bean Seed Extracts

For the determination of the total phenolic compound content, the conventional technique (Taga et al., 1984) was slightly modified, and 10 μl of the bean material was mixed with 200 μl of 2% Na2CO3 in a serum disk, and reacted at room temperature for 2 minutes. 10 μl of 50% Folin & Ciocalteau's phenolic reagent was shaken and mixed and allowed to react in the dark for 30 minutes at room temperature. Absorbance values at 750 nm were measured using a full wavelength microanalyzer (Molecular Devices, Spectra Max 250) and the standard curve was plotted as a gallic acid test.

For the determination of total flavonoids, the conventional technique (Jeng et al ., 2011) was slightly modified to take 20 μl of the bean material in sequence with 112 ml of double distilled water (ddH ₂ O), 60 ml of ethanol (95:5). , v/v), 4 ml of aluminum chloride (1:10, w/v) and 4 ml of 1 M potassium acetate were mixed evenly in the serum disk. The serum disk was allowed to stand at room temperature for 40 minutes in the dark, and the absorbance at 415 nm was measured with a full-wavelength microanalyzer (Molecular Devices, Spectra Max 250), and the standard curve was plotted with the quercetin test.

For the determination of total anthocyanin compounds, the conventional technique (Jeng et al ., 2010) was slightly modified to configure a buffer solution of 0.025 M potassium chloride (pH 1.0) and 0.4 M sodium acetate (pH 4.5), 1 ml of flowers. The bean materials were adjusted to pH 1.0 and pH 4.5 with a buffer solution, and the absorbance at 530 nm and 700 nm was measured after mixing uniformly. The formula for calculating the total anthocyanin content:

A = (A 530 nm - A700 nm) pH 1.0 - (A530 nm - A700 nm) pH 4.5.

MW = cyanidin-3-glucoside molecular weight 449.2.

V = sample volume.

DF = dilution factor.

ε = cyanidin-3-glucoside molecular weight extinction coefficient of 26,900.

The results of measuring the content of total phenols, total flavonoids and total anthocyanins in the polyphenolic compounds of the bean seed extract of the present example are shown in Table 1 below.

It can be seen from Table 1 that in view of the yield of the extract, the best in this example is to use the SA-05 strain as a plant material and extract it with a 50% aqueous solution of ethanol to obtain a higher content, and the total phenol content is about 2.08 to About 3.55 mg/g. Secondly, if you want to look at it in the future, it will be the best in spring.

Table 1

[Example 2] Inhibition of aldose reductase and α-glucosidase by flower bean seed extract

I. Determination of aldose reductase inhibitory activity of flower bean seed extract

(1) Pig water crystal aldose reductase extraction

The conventional technique (Saraswat et al., 2008) was slightly modified to purchase pig eyes from the market pig farmers on the same day, and the crystals were cut on ice. The following procedures were carried out at 4 ° C. The crystals were mixed with 3 volumes of 4 ° C 0.1 M phosphate buffer (0.1 MK ₂ HPO ₄ , 0.1 M KH ₂ PO ₄ , pH 7.0) and homogenized by homogenizer, 10,000×g. After centrifugation for 30 minutes, the supernatant was taken for the aldose reductase crude extract and stored at -80 ° C until use.

(II) Determination of aldose reductase inhibitory activity

The conventional technique (Fujita et al., 2004) was slightly modified to configure 0.05 M phosphate buffer (0.1 MK ₂ HPO ₄ , 0.1 M KH ₂ PO ₄ , pH 7.0) containing 0.01 M DL-glyceraldehyde (G5001, Sigma), 0.00015 M β-NADPH (N6505, Sigma), 20 μl of porcine water crystal aldose reductase crude extract and 10 μl of crotonol extract in a total volume of 1 ml. After uniformly mixing, it was placed in a 37 ° C water bath for 20 minutes, and 10 μl of ammonium chloride was added to terminate the reaction. 200 μl of the test product was taken in a serum disk, and the absorbance at 340 nm was measured with a full-wavelength microanalyzer (Molecular Devices, Spectra Max 250). Enzyme activity was defined as a change of 1 unit per 0.001 unit absorbance per minute at 37 °C.

II. Determination of α-glucosidase inhibitory activity by flower bean seed extract

The conventional technique (Kang at al ., 2012) was slightly modified, and 112 μl of 0.1 M phosphate buffer (pH 6.8), 20 μl of 0.2 unit/ml α-glucosidase (G0660, Sigma) and 8 μl were added to the serum disk. The bean seed extract was reacted at 37 ° C for 15 minutes. Then add 20 ml of 2.5 mM 4-nitrophenyl α-D-glucopyranoside (PNPG; N1377, Sigma), and after reacting at 37 ° C for 15 minutes, add 80 ml of 0.2 M Na ₂ CO _{3 to} mix and shake. The absorbance at 405 nm was measured with a full wavelength microanalyzer (Molecular Devices, Spectra Max 250). The standard curve is plotted at different concentrations of 4-nitrophenol (PNP). Enzyme activity definition: At 37 ° C, PNPG was induced to produce 1 μM PNP per minute at 1 unit.

III. Positive control group for inhibition of aldose reductase and α-glucosidase

In this example, the positive control group used acarbose, epalrestat, and ferulic acid. Acarbose is a second-type (non-insulin dependent) type of diabetes treated with an inhibitory drug designed for alpha-glucosidase. Epalrestat is an inhibitor against aldose reductase activity. Ferulic acid is a phenolic acid compound which also has an inhibitory activity against aldose reductase. The effect of each extract and the positive control group on inhibiting the activity of the enzyme is expressed by IC ₅₀ , which is defined as the content or amount of the extract when the enzyme activity is inhibited by 50%.

The results of the inhibition of the aldose reductase and the α-glucosidase in the bean seed extract of the present example are shown in Table 2 below.

Table 2 shows that, in the case of the polyphenol content in the extract, the inhibitory activity of the aldehyde reducing enzyme in the present embodiment is better than that of the SA-05 strain extracted with 50% aqueous ethanol solution, and the IC _{50 is} about At 10.95 μg/ml, the Hwachia is about 11.35 μg/ml. The best one is to extract the flower pod (Hwachia) with 80% aqueous methanol solution, and its IC ₅₀ is about 7.55 μg/ml. The extraction effect of the SA-05 strain is weak, and its IC ₅₀ is about 8.11 μg/ml.

In addition, in the above example, the α-glucosidase inhibitory activity is preferred in the present embodiment, and the SA-05 strain is extracted with an 80% aqueous methanol solution, and the IC ₅₀ is about 50.07 μg/ml. The flower pod (Hwachia) is about 58.93 μg/ml. The best one was to extract the SA-05 strain with 50% ethanol aqueous solution, the IC ₅₀ was 36.39 μg/ml, and the extraction effect of Hwachia was weak, and the IC ₅₀ was 43.21 μg/ml.

Each positive control IC ₅₀ values were determined as described later, acarbose ₅₀ α- glucosidase value of IC was 11.23 mg / ml, ₅₀ epalrestat aldose reductase value of the IC is 0.16 μg / Ml, ferulic acid also has an inhibitory activity on aldose reductase, and its IC ₅₀ is 8.44 μg/ml. Compared with the positive control group acarbose, the extract obtained by using 50% ethanol aqueous solution in the present embodiment has stronger α-glucosidase activity inhibiting ability, and should be used for replacing α-glucose to prepare α-glucose. A glycosidase inhibitor or a pharmaceutical composition comprising an effective amount thereof.

Briefly, in this embodiment, the SA-05 strain is preferably used as a plant material, and the obtained extract is superior to the selected flower pod (Hwachia) in inhibiting yeast α-glucosidase. If the difference of the inhibitory activities of the two enzymes involved in carbohydrate metabolism is compared between the extracts extracted from different solvents, the 80 mg aqueous methanol solution is preferred for the inhibition of porcine water aldose reductase; Preferably, the glycosidase inhibitory activity is selected from a 50% aqueous solution of ethanol.

[Example 3] Preparation method of flower bean seed extract containing α-amylolytic enzyme

I. Source of Bean Material

For the plant material used in this example, please refer to Example 1.

II. Preparation process of flower bean seed extract

The preparation method of this embodiment is a conventional technique (Kotaru et Al., 1987) slightly modified, please refer to the second figure. First, in step S21, the aforementioned bean seed is ground to obtain a single bean powder, and the preferred bean is selected from the group of flowers (Hwachia). The mutagenesis line obtained by mutagenesis of the flower pods by sodium azide was selected. In this example, the mutagenesis was selected from the SA-05 line.

Subsequently, in step S22, the soybean powder was dissolved using the first solvent, and the treatment was carried out in the dark and shaking at 150 rpm for 1 hour. The first solvent is selected from the following group: water, C ₁ -C ₄ alcohols, and combinations of any one. Preferably, in the present embodiment, the first solvent is an aqueous methanol solution, and the aqueous methanol solution is used in an amount of from about 70% by volume to about 90% by volume. The best one is 80% methanol aqueous solution. In another embodiment, preferably the first solvent is an aqueous ethanol solution, and the aqueous ethanol solution is used in an amount of from about 40% by volume to about 60% by volume. The best one is a 50% aqueous solution of ethanol.

Next, in step S23, the homogenized bean extract was centrifuged for 30 minutes, and a precipitate was collected, and the centrifugation condition was 10,000 × g.

In step S24, the precipitate was dissolved by adding double distilled water or a 0.5% sodium chloride (w/v) aqueous solution and treated under shaking at 120 rpm for 40 minutes to obtain a first crude extract.

Subsequently, in step S25, the first crude extract was centrifuged for 30 minutes, and then the supernatant portion was taken to obtain a second crude extract having a centrifugation condition of 10,000 × g.

Next, in step S26, the second crude extract was heated at 70 ° C for 15 minutes.

In step S27, the second crude extract was placed in an ice water bath for 15 minutes, and then placed in a refrigerator at 4 ° C for 30 minutes.

Finally, in step S28, the second crude extract was centrifuged for 30 minutes and then the supernatant fraction was taken to obtain a final adzuki bean extract, which was centrifuged at 10,000 x g. Among them, the final flower bean extract contains α-amylase inhibitor 1 (α-AI1) and can be stored at 4 ° C. The user may perform a freeze-vacuum drying process on the final flower bean extract to obtain a freeze-dried soybean extract, wherein the freeze-dried soybean extract may preserve several proteins.

III. Detection of protein content

10 μl of the above final bean extract was added to 200 μl of bradford reagent (B6916, Sigma), mixed uniformly and protected from light for 5 minutes, and the absorbance at 595 nm was measured with a full-wavelength microanalyzer (Molecular Devices, Spectra Max 250). Standard curves were prepared by diluting to different concentrations with 0.6 mg/ml calf serum albumin (Albumin bovine, BSA; A4503, Sigma).

[Example 4] Inhibition of α-amylolytic enzyme by flower bean seed extract

The conventional technique (Pueyo et al ., 1993) was slightly modified to configure 25 unit/ml porcine pancreatic alpha-amylolytic enzyme (A4268, Sigma) using SS buffer (15 mM NaOH, 20 mM CaCl ₂ , 0.5). M NaCl, pH 5.6). Take 100 μl of the extract and 100 μl of porcine pancreatic α-amylase in a centrifuge tube, mix well, and react in a 37 ° C water bath for 30 minutes, then add 600 μl of 2% starch solution (w/v, 20 mM sodium phosphate buffer, 6.7 mM NaCl, pH 6.9), reacted at 37 ° C for 1 minute. 800 μl of 3,5-dinitrosalicylic acid (DNSA) coloring reagent was added to the centrifuge tube and mixed well. It was placed in a 95 ° C boiling water bath for 12 minutes and allowed to cool at room temperature. A standard curve was prepared by diluting to a different concentration with a 1% maltose solution. After the sample was diluted 6 times with double distilled water, the absorbance at 530 nm was measured with a full wavelength microanalyzer (Molecular Devices, Spectra Max 250).

The results of the inhibition of the α-amylolytic enzyme in the bean seed extract of the present example are shown in Table 3.

Table 3 shows that, in the case of the polyphenol content in the extract, the inhibition effect of the α-amylase-degrading activity of the present embodiment was better than that of the SA-05 strain extracted with the 80% aqueous methanol solution. In addition, based on the weight of the extracted dry matter, the best solvent for α-amylase decomposing activity in this example is 80% methanol aqueous solution and 0.5% sodium chloride (w/v) aqueous solution. The SA-05 strain extracted as the second solvent had the best inhibitory effect and had an IC _{50 of} about 17.68 μg/ml. The second best method is to extract the first solvent using a 50% aqueous solution of ethanol and a 0.5% sodium chloride (w/v) aqueous solution as the second solvent, and the obtained IC ₅₀ is about 31.11 μg/ml. Approximately 38.68 μg/ml.

If the inhibitory effect between different extracts is further compared based on the protein content, it can be known that when the SA-05 strain is selected as the plant material and the 0.5% sodium chloride (w/v) aqueous solution is used as the second solvent for extraction, Indeed, it is helpful to obtain a protein containing high activity α-AI1, and the resulting extract has an IC _{50 of from} about 0.17 μg/ml to about 0.24 μg/ml. In the present example, the IC ₅₀ value of the acarbose as a positive control group was about 3.70 μg/ml, and in comparison, the above-mentioned extract was more effective than acarbose.

[Example 5] Food and beverage and pharmaceutical composition containing flower bean seed extract

In a specific embodiment, the present invention provides a food or drink for regulating blood sugar in a mammal comprising an effective amount of the above extract.

In view of the above-described evidence for the inhibitory effect of the bean seed extract of the present invention on aldose reductase, alpha-glucosidase and alpha-amylolytic enzyme, in another embodiment, a use of the above extract is provided. The use of a pharmaceutical agent for regulating blood sugar in a mammal. Also provided is a pharmaceutical composition for modulating a blood glucose cell of a mammal comprising an effective amount of the above extract.

In yet another embodiment, a method for regulating blood sugar in a mammal is also provided A pharmaceutical composition comprising an effective amount of the above extract and at least one saccharide metabolism related inhibitor.

In particular, the pharmaceutical compositions of the present invention can be administered in any convenient manner, for example, but not limited thereto, for example, by oral, subcutaneous or intravenous administration. The pharmaceutical composition can be used alone or in combination with a pharmaceutical adjuvant, and can be used in veterinary and human medicine.

For example, in the preparation of a pharmaceutical preparation suitable for oral administration, an adjuvant which does not adversely affect the activity of the extract may be contained in the pharmaceutical composition of the present invention, for example, a solvent, an oily solvent, a diluent, a stabilizer, an absorption delaying agent, and a disintegration. Powder, emulsifier, binder, lubricant, moisture absorbent, etc. For example, the solvent may be selected from the group consisting of water and sucrose solutions, the diluent may be selected from the group consisting of lactose, starch, and microcrystalline cellulose, the absorption delaying agent may be selected from the group consisting of chitosan and aglycosyl glycans, and the lubricant may be selected from the group consisting of Magnesium, an oily solvent may be selected from plant or animal oils such as olive oil, sunflower oil and cod liver oil. The composition can be formulated into a suitable oral administration form by a conventional method, for example, a tablet, a capsule, a granule, a powder, a flow extract, a solution, a syrup, a suspension, an emulsion, an expectorant, and the like. Wait.

For pharmaceutical preparations suitable for subcutaneous or intravenous administration, one or more ingredients such as solubilizers, emulsifiers, and other adjuvants may be included in the pharmaceutical composition of the present invention to prepare, for example, intravenous infusion solutions, emulsion veins. Infusion solution, injection, dry powder injection, suspension injection, or dry powder suspension injection. Solvents which may be employed are, for example, water, physiological saline solutions, alcohols (e.g., ethanol, propanol, or glycerol, etc.), sugar solutions (e.g., glucose or mannose solutions), or combinations of the foregoing.

Optionally, the pharmaceutical composition of the present invention may further contain additives such as a flavoring agent, a toner, a coloring agent, etc., to improve the mouthfeel and visual feeling when the obtained medicament is taken; and a reasonable amount of preservative, preservative, An antibacterial agent, an antifungal agent or the like to improve the storage property of the obtained agent.

The content of the extract in the pharmaceutical composition of the present invention can be adjusted according to the age of the object to be administered and the purpose of application (for example), and the frequency of use can be adjusted as needed.

Furthermore, it may be desirable to include one or more other active ingredients in the pharmaceutical compositions of the present invention to further enhance the efficacy of the pharmaceutical compositions of the present invention or to increase the flexibility and formulation of the formulation. For example, the pharmaceutical composition of the present invention may contain one or more active ingredients related to the regulation of carbohydrate metabolism in the body: acarbose, epalrestat, ferulic acid and others. The active ingredient or the like as long as the other active ingredient does not adversely affect the benefit of the extract.

The above is only a preferred embodiment of the present invention, and equivalent changes to the scope of the present invention and the scope of the patent application are intended to be included in the scope of the present invention.

The first figure shows a flow chart of a specific embodiment of the method for preparing the bean seed extract of the present invention; the second figure shows a flow chart of another specific embodiment of the method for preparing the bean seed extract of the present invention;

Claims (40)

A seed extract of Phaseolus vulgaris L. is prepared by the method comprising the steps of: a) grinding the bean seed to obtain a flower bean powder; b) dissolving the bean powder using a first solvent to Obtaining a homogenous soybean extract; and c) centrifuging the homogenous soybean extract and collecting a supernatant of the soy bean extract, wherein the step b) is dissolved in the first solvent 10 by about 1 gram of the soybean powder The ratio of milliliters is carried out. The extract of item 1, wherein the first solvent is selected from the group-based request: water, C ₁ -C ₄ alcohols, and combinations of any one. The extract according to claim 1, wherein the first solvent is an aqueous ethanol solution, and the aqueous ethanol solution is from about 40% by volume to about 60% by volume. The extract of claim 1 is a 50% aqueous ethanol solution (v/v). The extract according to claim 1, wherein the first solvent is an aqueous methanol solution, and the aqueous methanol solution is from about 70% by volume to about 90% by volume. The extract of claim 1 is the 80% aqueous methanol solution (v/v). The extract of claim 1, wherein the dissolving process of the step b) is carried out in the dark and shaking at 150 rpm for 1 hour, and the centrifugation condition of the step c) is centrifugation at 10,000 x g for 30 minutes. The extract of claim 1, wherein after step c), a filtration step d) is further included to obtain a purified flower bean extract. The extract of claim 1, wherein the flower bean is selected from the group consisting of a flower pod (Hwachia) or a mutagen strain obtained by mutagenizing the flower pod with sodium azide. The extract of claim 9 is selected from the group consisting of the flower pods. The extract of claim 9 is selected from the mutagen line. As the extract of claim 11, the mutagen is the SA-05 line. The extract of claim 10, wherein the supernatant of the soy bean extract comprises a polyphenolic compound having a total content concentration of about 1.25 mg/kg of the bean seed to about 3.13 mg/kg. Flower bean seeds. The extract of claim 13, the polyphenolic compound comprising a flavonoid compound and an anthocyanin compound. The extract of claim 11, wherein the supernatant of the soy bean extract comprises a polyphenolic compound having a total content concentration of about 1.62 mg/kg of the bean seed to about 3.69 mg/kg. Flower bean seeds. The extract of claim 15, the polyphenolic compound comprising a flavonoid compound and an anthocyanin compound. The extract of claim 3, wherein after the step d), further comprises a freeze-vacuum drying step e) to obtain a freeze-dried soybean extract, wherein the freeze-dried soybean extract comprises at least one protein. The extract of claim 17, wherein the extract has a total protein weight percentage of between about 52 and 95 mg per gram of bean seed. The extract of claim 17, wherein the extract has a total protein weight percentage of between about 0.29 and 1.9 mg per gram of flower bean seed. The extract of claim 10 having an activity of inhibiting aldose reductase having an IC ₅₀ of from about 0.36 mg/ml to about 0.40 mg/ml. The extract of claim 12 having an activity of inhibiting aldose reductase having an IC ₅₀ of from about 0.39 mg/ml to about 0.46 mg/ml. The extract of claim 10 having an activity of inhibiting α-glucosidase having an IC ₅₀ of from about 1.46 mg/ml to about 1.92 mg/ml. The extract of claim 12, which has an activity of inhibiting α-glucosidase, having an IC ₅₀ of from about 1.32 mg/ml to about 1.94 mg/ml. A food or drink for regulating blood sugar of a mammal, comprising an effective amount of the extract according to any one of claims 1 to 23 and at least one food additive. A pharmaceutical composition for modulating blood glucose in a mammal comprising an effective amount of the extract of any one of claims 1 to 23 and at least one pharmaceutically acceptable excipient. Use of a flower bean seed extract according to any one of claims 1 to 23 for the manufacture of a medicament, wherein the medicament is for regulating blood sugar in a mammal. A pharmaceutical composition for modulating blood glucose in a mammal comprising an effective amount of the extract of any one of claims 1 to 23, a metabolic inhibitor, and at least one pharmaceutically acceptable excipient. The pharmaceutical composition according to claim 27, wherein the metabolic inhibitor is selected from the group consisting of an aldose reductase inhibitor, an α-glucosidase inhibitor, an α-amylase inhibitor, and a combination thereof. A method for preparing a flower bean seed ( Phaseolus vulgaris L.) extract comprises: a) grinding the bean seed to obtain a flower bean powder; b) dissolving the bean powder using a first solvent to obtain a homogenous bean And extracting the homogenized bean extract and collecting a supernatant of the soy bean extract, wherein the flower bean is selected from the group consisting of a flower pod (Hwachia) or the flower pod is obtained by mutagenesis of sodium azide The mutagenesis is a strain of SA-05, and the step b) is carried out at a ratio of 1 gram of the soybean powder dissolved in 10 ml of the first solvent, and the first solvent is selected from the group consisting of C ₁ -C ₄ alcohol solutions . The preparation method of claim 29, wherein the first solvent is an aqueous methanol solution, and the aqueous methanol solution is from about 70% by volume to about 90% by volume. In the preparation method of claim 29, the first solvent is an aqueous ethanol solution, and the aqueous ethanol solution is from about 40% by volume to about 60% by volume. The extract of claim 29, wherein the dissolving process of step b) is carried out in the dark and shaking at 150 rpm for 1 hour, and the centrifugation condition of step c) is 10,000 x g for 30 minutes. The preparation method of claim 29, wherein after the step c), further comprising a filtration step d) to obtain a purified flower bean extract. The preparation method of claim 29, wherein the supernatant bean extract comprises a polyphenol compound having a total content concentration of about 1.25 mg/kg of the bean seed to about 3.13 mg/kg. Flower bean seeds. A method for preparing a seed extract of a flower bean ( Phaseolus vulgaris L.) is prepared by the method comprising the steps of: a) grinding the bean seed to obtain a flower bean powder; b) dissolving the flower bean using a first solvent Powder to obtain a homogeneous bean extract; c) centrifuging the homogenized bean extract and collecting a precipitate; d) adding a second solvent to dissolve the precipitate to obtain a first crude extract; e) centrifuging the first a crude extract and taking the supernatant portion thereof to obtain a second crude extract; f) heating the second crude extract; g) cooling the second crude extract; and h) centrifuging the second crude extract and Taking the supernatant portion thereof to obtain a final flower bean extract, wherein the flower bean is selected from a seed pod (Hwachia) or a mutagen strain SA-05 obtained by mutagenesis of the flower pod species and This step b) is carried out at a ratio of 1 g of the edulis powder dissolved in 10 ml of the first solvent. The preparation method of claim 35, wherein the first solvent is an aqueous methanol solution, and the aqueous methanol solution is from about 70% by volume to about 90% by volume. In the preparation method of claim 35, the first solvent is an aqueous ethanol solution, and the aqueous ethanol solution is from about 40% by volume to about 60% by volume. The preparation method of claim 35, wherein the second solvent is distilled water. The preparation method of claim 35, wherein the second solvent is a 0.5% sodium chloride (w/v) aqueous solution. The dissolution process of the step b) is carried out in the dark and shaking at 150 rpm for 1 hour, and the dissolution process of the step d) is carried out under the condition of shaking at room temperature 120 rpm for 40 minutes. The heating process of the step f) is carried out under the condition of a temperature of 70 ° C for 15 minutes, and the cooling process of the step g) is carried out at a temperature of 4 ° C. The centrifugation process of the step h) is performed at a centrifugal force of 10,000 × g. It was carried out under 30 minutes.