JP2007300869A - Highly functional health food and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide highly functional health food that shows the physiological activity at equal or higher level to or than that of the conventional one with a smaller amount of effective dose through highly activating the physiological activity of health food raw material. <P>SOLUTION: This highly functional health food comprises a mixture prepared by mechanically dispersing and treating the health food raw material and a phospholipid derived from a natural product, wherein the health food material comprises at least one kind selected from bitter gourd extract, Orthosphon aristatus extract, fiddlewood extract, mulberry leaf extract, Citrus depressa Hayata extract, loofah extract, Peucedanum japonicum extract, Citrus tankan Hayata extract, Crepidiastrum lanceolatum extract or Morinda citrifolia extract, and the phospholipid derived from the natural product comprises at least one kind selected from an egg yolk phospholipid or a soybean phospholipid. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a health food in which the physiological activity of a health food material is highly activated, and in particular, bitter extract, cat beard extract, pine wig extract, mulberry leaf extract, shik shisha extract, loofah extract, button A highly functional health food that enhances the physiological activity possessed by Bow Fu extract, Tankan extract, Hosova budan extract, or Noni extract, and exhibits a physiological activity equal to or higher than a conventional effective amount, And a method for producing a highly functional health food.

  Health foods are foods that claim a good health effect (biological adjustment function), and generally refer to foods that are widely sold and used as foods that contribute to the maintenance and promotion of health. In recent years, there has been a growing interest in the health of the nation due to nutritional bias due to disordered eating habits, diversification of eating habits, an increase in obesity and lifestyle-related diseases, and aging, etc. There is an increasing need for them.

  The general health effects expected from these health foods are: immunity enhancing action, antiviral action, antitumor action, hypoglycemic action, arteriosclerosis improvement, anticholesterol action, blood pressure lowering action, antipyretic action, intestinal regulation Action, diuretic action, analgesic action, antibacterial action, hangover prevention action and the like.

  On the other hand, general physiologically active ingredients considered to be related to the health effects expected of health foods include monosaccharides, disaccharides, functional oligosaccharides, polysaccharides, fatty acids, glycolipids, steroids, amino acids, Peptides, proteins, fat-soluble vitamins, water-soluble vitamins, vitamin-like active substances, minerals, dietary fiber, terpenoids, tannins, flavonoids, carotenoids, organic sulfur compounds, organic acids, chlorophyll, fermenting microorganisms (probiotics), and other special Components and the like.

  However, when these physiologically active ingredients are ingested orally, they are affected by various physical properties of the ingredients or in the body environment, leading to a decrease in bioavailability and it may be difficult to obtain the expected effects. There was a problem that there was.

  Here, the bioavailability can be considered as the amount transferred to the blood / total dose of physiologically active components such as orally administered drugs. Bioavailability depends on drug solubility, particle properties, physical and chemical degradation of the drug, absorption in the digestive tract, and the like. The higher the bioavailability, the better the absorption of drugs and the like, and the expected medicinal effect is expected.

For example, since polysaccharides and proteins, which are physiologically active ingredients, have a relatively large molecular weight, they have a problem of poor membrane permeability from the gastrointestinal mucosa and are difficult to be absorbed from the gastrointestinal tract when orally administered.
Therefore, for the purpose of obtaining the physiological activity of polysaccharides by ingestion at present, a method to increase the absorption efficiency from the digestive tract by subjecting polysaccharides and proteins to chemical treatment and enzyme treatment to reduce their molecular weight In addition, a method for increasing the amount of absorption by ingesting a large amount of polysaccharides and proteins has been studied.

  However, it is not clear whether the expected physiological activity is expressed when the molecular weight of the polysaccharide or protein is reduced to a molecular weight lower than that of the oligosaccharide or peptide. Moreover, since a large amount of intake is accompanied by human suffering, the intake itself is difficult and there is a problem that it is not desirable as a health food.

On the other hand, lipophilic physiologically active ingredients have a problem of poor membrane permeability from the gastrointestinal mucosa and, like polysaccharides, are difficult to be absorbed from the gastrointestinal tract when administered orally.
Therefore, at present, as a method for obtaining the physiological activity of a physiologically active ingredient having a lipophilic component by oral ingestion, a method in which the lipophilic component is encapsulated together with oil (for example, see Patent Document 1), A method of ingesting a large amount of lipophilic components has been taken.

JP 2001-086931 A

However, since the method of ingesting a large amount of lipophilic components is accompanied by human pain, the ingestion itself is difficult. Also, due to the large amount of intake, complicated food processing techniques such as thick retort foods, capsules, special granule products, and tablet products are required for processing and manufacturing, and the number of manufacturing processes is increased. There is a problem that
Furthermore, even when lipophilic components are encapsulated and ingested with oil, there are problems such as the need for complicated food processing techniques and an increase in the manufacturing process, and these methods may cause considerable manufacturing costs. Currently.

In addition, some physiologically active ingredients are degraded by digestive fluids such as gastric acid and duodenum produced from the stomach and duodenum after ingestion by mouth, so that most of them are not absorbed by the small intestine. .
A technique for absorbing a physiologically active component without being decomposed from the digestive tract has been a problem for a long time, but no technique has been established yet. For example, the following methods are known as techniques for promoting oral absorption of physiologically active ingredients.

For example, nonionic interfaces such as bile salts having a surfactant activity (WO 9606635), sucrose fatty acid esters, and O-acyl-L-carnitines having an acyl group having 8 to 18 carbon atoms (US Pat. No. 4,537,772). A method of using an active agent or an anionic surfactant such as sodium lauryl sulfate (SLS), or a method of using bile salts and SLS in combination (Pharm. Res., 7, No. 9, Suppl., S157, 1990). This method is a method for promoting the absorption of a highly water-soluble substance because it increases the fluidity of the cell membrane, but has not yet been put into practical use due to the problem of cytotoxicity (Journal).
of Controlled
release, 29, 253, 1994).

  EDTA (J. Pharm. Pharmacol., 51, No. 11, 1241-50, 1999), chelating agents such as EGTA, or trypsin inhibitor (J. Pharm. Pharmacol., 50, No. 8, 913-20, (1998) and the like. This method is a method in which the cell gap is loosened by extracting calcium ions, and the membrane permeability of the polymer substance is promoted. However, at physiological pH (neutral), the absorption effect must be relatively high. Is not expressed, and mucosal damage due to withdrawal of calcium ions has been reported.

In view of the above situation, the present inventors have intensively studied food processing technology that promotes absorption of lipophilic components and protects them from degradation by gastric acid and pancreatic juice without reducing the molecular weight of active ingredients such as polysaccharides and proteins. did. And among the natural food materials each having the above problems, bitter melon extract, cat beard extract, pine wig extract, mulberry leaf extract, shikushasha extract, loofah extract, button boo fu extract, It has been found that there is an action to solve the above problems by mixing at least one selected from a tankan extract, a Hosovabadan extract, or a noni extract with at least one selected from a phospholipid derived from a natural product. It came to be completed.
The present invention provides a highly functional health food that highly enhances the physiological activity of a health food material, and exhibits a physiological activity equal to or greater than a conventional effective amount, and a method for producing a highly functional health food. The purpose is to provide.

  In order to achieve the above object, the highly functional health food of the present invention is a highly functional health food comprising a mixture of a health food material and a phospholipid derived from a natural product mechanically dispersed, Health food material is from bitter melon extract, cat beard extract, pine beetle extract, mulberry leaf extract, shikshusha extract, loofah extract, button bowfish extract, tankan extract, hosobabadan extract, or noni extract The natural product-derived phospholipid is at least one selected from egg yolk phospholipid and soybean phospholipid.

If such a high-performance health food is configured as described above, these health food materials are mixed with egg yolk phospholipids or soybean phospholipids and mechanically dispersed to provide a living body that each of these health food materials has. The adjustment function can be improved.
For this reason, according to the highly functional health food of the present invention, it is possible to obtain a health effect equivalent to or higher than the conventional amount in a smaller amount than the conventional effective amount.

Moreover, the highly functional health food of the present invention is such that the above mixture contains bittern.
This makes it possible to further increase the physiological activity of each health food material.

In the highly functional health food of the present invention, the mechanical dispersion treatment is at least one selected from a high-speed rotational dispersion treatment, an ultrasonic dispersion treatment, and a high-pressure homogenizer dispersion treatment.
It is possible to further improve physiological activity by obtaining a high-functional health food by performing such mechanical dispersion treatment.

In addition, the method for producing a highly functional health food of the present invention comprises a bitter melon extract, a cat beard extract, a pine wig extract, a mulberry leaf extract, a shik sushi extract, a loofah extract, a button boo extract, and a tankan extract. Obtained by mixing a health food material consisting of at least one selected from foods, Hosoubadan extract, or noni extract and phospholipids derived from natural products consisting of at least one selected from egg yolk phospholipid or soybean phospholipid This is a method for performing high-speed rotational dispersion treatment of a mixture.
If the manufacturing method of high-functional health food is such a method, these health food materials are mixed with egg yolk phospholipids or soybean phospholipids and mechanically dispersed. A highly functional health food with an improved biological adjustment function can be manufactured.
For this reason, it becomes possible to manufacture the highly functional health food which can acquire the health effect equivalent to or more than before with a small amount from the conventional effective amount.

Moreover, the manufacturing method of the highly functional health food of this invention exists as a method of mixing a bittern further in the said mixing.
Moreover, the manufacturing method of the highly functional health food of this invention is a method of performing the ultrasonic dispersion process or the high-pressure homogenizer dispersion process of the said mixture after the said high speed rotation dispersion process.
If the manufacturing method of a highly functional health food is made into such a method, it will become possible to manufacture the highly functional health food which further activated the physiological activity of each health food material.

According to the present invention, a bitter melon extract, a cat beard extract, a pine wig extract, a mulberry leaf extract, a seek squirrel extract, a loofah extract, a button bowfish extract, a tankan extract, a Hoso Bawadan extract, or a noni extract By mixing a phospholipid with a product and subjecting it to a mechanical dispersion treatment, a higher physiological activity can be obtained with a smaller intake amount.
That is, it becomes possible to expect the below-mentioned physiological activity which each of these health food materials has in an amount less than the conventional effective amount.

Hereinafter, a highly functional health food according to an embodiment of the present invention will be described. Note that the configuration described below does not limit the present invention and can be variously modified within the scope of the present invention.
The highly functional health food of the present invention includes bitter melon extract, cat beard extract, pine pod extract, mulberry leaf extract, shik sushi extract, loofah extract, button bow fu extract, tankan extract, Hoso Bawadan extract Or at least one health food material selected from the group consisting of noni extracts and at least one natural phospholipid selected from egg yolk phospholipid or soybean phospholipid, and the resulting mixture is machined Distributed processing.

The health food of the present invention containing each of the above health food materials and naturally derived phospholipids has the physiological activity expected of the physiologically active ingredients contained in each of the health food materials. Further, by combining these health food materials, further physiological activity can be expected.
In addition, as the health food material of the present invention, an extract such as an extract can be used. In addition, the extract contains sufficient extract components such as dry powder of button fountain, and has the same effect as the extract. A highly functional health food containing a material obtained from the above as a health food material and a method for producing the same are within the scope of the present invention.

(Anguish)
Okinawa's special product, bitter melon, is called Momordica charantia L and scientific name is Goya, and it grows by extending its stem so that it is entangled with hermaphrodite and squirrel in an annual herb of cucurbitaceae. The flowers are hermaphrodite, with yellow pretty flowers from around May and June in summer, and the fruits are thick cucumbers covered with warts and are mainly used as vegetables. It is a vegetable with a bitter taste, as it is called bitter melon. The variety of bitter melon cultivated in Okinawa is a kind of long-necked peach.
The agony was transmitted to China from the south in the Ming era, and it came to Japan during the Tokugawa era. It has been widely known as a healthy vegetable king since ancient times. Generally, it is said that there are effects such as blood pressure stabilizing action, healthy stomach action, diuretic action, bowel regulation action and the like.

  Ingredients contained in bitter melon include vitamin C, potassium, saponin, charanthin, momordesin, adenine, citrulline and the like.

Examples of methods for adjusting the bitter melon extract include the following methods.
First, after pulverizing 100 kg of bitter melon washed as a raw material, it is blanched at about 90 ° C. for 1 to 1.5 hours. Then, the mixture is cooled to 45 ° C., 50 g of cellulolytic enzyme (enzyme titer: 1500 U / g) and 50 g of pectin degrading enzyme (enzyme titer: 1000 U / g) are added, and the mixture is stirred for 2 to 2.5 hours. Enzyme extraction. Thereafter, the enzyme is inactivated while stirring at 90 ° C. for 15 minutes. The extract is separated into solid and liquid by a centrifuge. The bitter extract is obtained by removing the solid part out of the system.
There are other methods such as hot water extraction with hot water (about 90 ° C.) and alcohol extraction with ethanol (about 70 ° C.).

(Loofah)
Loofah is an annual plant of the Cucurbitaceae family, native to tropical Asia. It came to Japan at the beginning of the Edo period and became widely cultivated. From summer to autumn, five yellow petals with a diameter of 5 to 10 cm are opened, and the fruits are elongated green and dark green.
When fruits ripen, strong reticulated fibers develop. Since fruits contain abundant alcohol-degrading enzymes and vitamin C, which are water-soluble components, hangover prevention and whitening effects can be expected.

  Examples of physiologically active components contained in loofah include terpenoids such as lucine and lucioside, vitamin C, alcohol degrading enzyme (ADH), and aldehyde degrading enzyme (ALDH).

Examples of methods for adjusting loofah extract include the following methods.
First, 5 kg of washed loofah as a raw material is cooled to about 5 ° C. Next, the cooled loofah is cut with a cutter or the like, put into a home mixer, 1000 ml of cold water at around 5 ° C. is added, and pulverized for 2 to 3 minutes at full rotation. Subsequently, the crushed loofah is centrifuged at 8000 rpm for 20 minutes using a centrifuge. And only a supernatant liquid is taken out and a solid part is removed. Sodium hydroxide (NaOH) is added to the supernatant obtained by solid-liquid separation to adjust the supernatant within the range of pH 6.9 to pH 7.1, followed by filtration under reduced pressure. A loofah extract is obtained.
In addition to this, there are methods of extracting water with water (5 ° C. to 90 ° C.) and squeezing a cut loofah by applying a pressure of about 350 kg / cm ² .

(Kumamatsura)
Kumagusura is called the Okinawan name "Machinokusa" and its scientific name is "Verbena officinalis L." It is a perennial plant that is widely distributed from the warm zone to the tropics in Honshu, Shikoku, Kyushu, Okinawa, Asia, Europe, and North Africa, and that grows in the wilderness and on the roadside. Stand upright at a plant height of 60 to 100 cm and branch upward. In addition to anti-inflammatory diuretic activity, the extract of hot water extract of pine moth has reported analgesic and antibacterial effects.

  Examples of components contained in the pine pod include verbanol, a pyran derivative, and verbenaline, berbenalol, and berbenine, which are glycosides thereof.

Examples of methods for adjusting the extract of the pine wig include the following methods.
First, the honeysuckle is added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable under the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane, or the like alone or in admixture of two or more.
In addition to this, there is also a method of obtaining a juice by applying a pressure of about 350 kg / cm ² to the cut wig and squeezing it.

(Tankan)
Tankan belongs to the citrus citrus family, and its scientific name is Citrus tankan HAYATA. Tankan is native to Guangdong Province, China, and is said to be a natural hybrid of orange and Ponkan. It is a member of Tangor, represented by Iyokan and Kiyomi. Tankan is rich in fruit juice, good in flavor, few seeds, slightly smaller than orange, has a heavy feeling, and is characterized by its sweetness wrapped in moderate acidity. A sympathomimetic action, a carcinogenesis-inhibiting action, a blood glucose level-inhibiting effect, an antioxidant action, etc. have been reported.

  The components contained in the tankan include catecholamines such as vitamin C and synephrine, carotenoids such as β-cryptoxanthin, polymethoxyflavones such as nobiletin and tangerine, phenylpropanoids such as caffeic acid, and coumarins such as aurapten. Is mentioned.

Examples of methods for adjusting the tankan extract include the following methods.
First, tankan is added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.
In addition to this, there is also a method of obtaining a juice by applying a pressure of about 350 kg / cm ² to the tankan and pressing it.

(Seek Shear)
Shikshasha belongs to the citrus citrus family, and its scientific name is Citrus depressa HAYATA. A small mandarin that is wild from the Nansei Islands to the mountains of Taiwan, has a unique aroma and taste, and is used for cooking and raw food in the region. In recent years, animal experiments have revealed cancer prevention effects, improvement of insulin resistance, blood glucose level suppression effects, blood pressure suppression effects, and the like.

  Examples of the physiologically active component contained in Seek Shear are flavonoids such as nobiletin or tangeretin.

For example, the following methods can be used to adjust the seeker extract.
First, a seek shaker is added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.
In addition to this, there is also a method of obtaining a juice by applying a pressure of about 350 kg / cm ² to a seek shear and pressing it.

(Cat beard)
Nekonohige is a perennial plant belonging to the family Lamiaceae native to Southeast Asia with the scientific name Orthosiphon stamineus Benth. In Malay, Kumis is also called Kumiskutin because it means beard and Kuching means cat. In Japan, it is cultivated in warm regions such as Tanegashima, mainly in Okinawa. The extract of cat beard has been confirmed to have diuretic action, blood pressure lowering action and the like.

  Examples of the component contained in the cat beard include benzopyrans such as potassium and methyllipariochromene A, and phenols such as rosmarinic acid.

For example, the following methods are available as methods for adjusting the feline beard extract.
First, a cat beard is added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.

(Hosobawa Dan)
Hosovawadan is a plant belonging to the genus Azetouna, whose name is Okinawa, and whose scientific name is Crepidiastrum lanceolatum. It is a perennial that grows from the Sea of Japan in Shimane Prefecture and Yamaguchi Prefecture in Honshu to Ryukyu, southern Korea, and rocky places on the coast of China, and is said to have blood pressure lowering, whitening, antioxidant, and immunostimulatory effects. .

  Examples of the components contained in Hosoubadan include water-soluble polyphenols such as caffeic acid, chicory acid, chlorogenic acid, and isochlorogenic acid, and flavonoids such as luteolin and luteolin glucoside.

For example, the following methods can be used to adjust the Hoso Babadan extract.
First, Hosoubadan is added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.

(mulberry)
Mulberry belongs to the genus Mulberry, and its scientific name is Morus australis Poir. It is a deciduous tree that is widely distributed from the tropics to the subtropics. In Japan, there are three types of cultivars, Yamaguchi, Karayamaguwa and Logwa, with over 100 varieties. The mulberry leaves dried in the shade are called “Shinsen tea” and are said to be effective in suppressing blood sugar, nourishing, and suppressing blood pressure.

  In mulberry leaves, in addition to steroids such as carotenes and ergosterol, there is a water-soluble component called 1-deoxynojirimycin (hereinafter abbreviated as 1-DNJ) having a structural formula very similar to glucose. include.

Examples of methods for adjusting the mulberry leaf extract include the following methods.
First, mulberry leaves are added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.

(Button bow)
Button Bow Fu is a plant of celery that is called the Okinawan name “Long Life Grass” and the scientific name “Peucedanum japonicum Thunb”. It is an evergreen perennial plant that grows naturally in harsh natural conditions such as coastal cliffs and rocks made of limestone peculiar to Okinawa Prefecture. Button fountain has been shown to have a hypotensive effect, an antioxidant effect, an improvement in neuralgia, and an antipyretic effect.

  In the leaf of button pea, flavonoids such as rutin are contained in addition to polyphenols such as chlorogenic acid and neochlorogenic acid.

Examples of methods for adjusting the button bow extract include the following methods.
First, button bow is added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.

(Noni)
Noni has the scientific name Morinda citrifolia L and is also called Indian Mulberry. Noni can be shrubs 3-10 meters high or small or medium sized trees. Noni has some rounded branches and leaves that are evergreen (or quasi-alternating), dark, glossy, and wavy protruding veins. Noni leaves are wide oval to rectangular with both ends pointed, 10-30 cm long and 5-15 cm wide.
Noni fruits, like potatoes, have “eyes” on their surface. This fruit is hydrated, bitter, dark yellow to yellowish white, has many reddish brown hard, oval to triangular winged two-cell nuclei, each containing about 4 species ing. When the fruit is fully ripe, it has a strong smell of rotten cheese. This fruit extract has anti-cancer activity.

  Noni fruits contain many enzymes called prozeroin. This pro-zeroin has been shown to convert to xeronine in the body, repair and regenerate damaged cells in the body, and enhance the immunity of contaminated cells to normalize them.

Examples of methods for adjusting noni extract include the following methods.
First, noni fruits are added to a predetermined extraction solvent. Then, after performing extraction at a predetermined temperature for a predetermined time, an extraction extract can be obtained by removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.
In addition to this, there is also a method of obtaining a juice by applying a pressure of about 350 kg / cm ² to Noni fruits and pressing them.

  The health food material is preferably used mainly for the highly functional health food of the present invention mainly in the form of an extract, but is not limited to the extract. That is, in addition to the extract, the health food material may be squeezed and the resulting juice may be used, or one extracted with water or a predetermined organic solvent may be used.

  When using an organic solvent for extraction, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, ethylmethyl Use ones acceptable for foods such as ketones, glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible fats and oils, 1,1,1,2-tetrafluoroethane, 1,1,2-trichloroethene. In particular, it is more preferable to use hot water, hydrous ethanol, hydrous propylene glycol or the like.

(Phospholipid)
Examples of the phospholipid used in the present invention include egg yolk phospholipid, soybean phospholipid, and the like. In addition to using one of these, both can be used in combination.
Moreover, in this invention, what processed these with the predetermined enzyme, or these hydrogenated substances can also be used.
Furthermore, as these phospholipids, those generally used as a food additive is desirable. Further, the purity is preferably 30% or more, and more preferably 80% or more.

(Production method)
Next, the manufacturing method of the highly functional health food of this invention is demonstrated.
The method for producing a highly functional health food according to the present invention includes a bitter melon extract, a black beard extract, a pine pod extract, a mulberry leaf extract, a shik shusha extract, a loofah extract, a button boo extract, a tankan extract, A health food material consisting of at least one selected from Hosovawadan extract or Noni extract and a phospholipid derived from a natural product consisting of at least one selected from egg yolk phospholipid or soybean phospholipid, and a mixture obtained Although it is not particularly limited as long as high-speed rotation dispersion processing (high-speed rotation processing) can be performed, for example, as follows, a method including an extraction step, a preliminary dispersion processing step, and a dispersion processing step It is preferable to do.

[Extraction process]
In the extraction step, the extract of each material can be extracted using the extract adjustment method described above for each health food material.
For example, an extract can be obtained by adding a health food material to a predetermined extraction solvent, performing extraction for a predetermined time at a predetermined temperature, and then removing the extraction residue. The extraction solvent is not particularly limited as long as it is acceptable in accordance with the Food Sanitation Law. For example, it is preferable to use hot water, ethanol, acetone, hexane or the like alone or in combination.
In addition, there is a method of obtaining a juice by applying a pressure of about 350 kg / cm ² to the health food material and pressing it.

[Preliminary dispersion process]
First, the health food material is added to water and preliminarily dispersed by mechanical stirring. At this time, the concentration of the health food material can be, for example, less than 20 g / 100 ml, and preferably 1 to 10 g / 100 ml.
This preliminary dispersion treatment is preferably performed, for example, at 1000 to 4000 rpm with a high-speed rotating disperser for about 5 to 15 minutes. Moreover, the processing temperature can be about 20-70 degreeC, for example, and it is preferable to set it as 30-60 degreeC.

Alternatively, phospholipid is added to water and predispersed by mechanical stirring. At this time, the concentration of the phospholipid can be, for example, less than 30 g / 100 ml, and preferably 1 to 20 g / 100 ml. The preliminary dispersion is preferably performed under the same conditions as the health food material.
Then, the phospholipid dispersion liquid in which the phospholipid is predispersed is added to the health food material dispersion liquid in which the health food material is predispersed while maintaining the same mechanical agitation as in the predispersion treatment. The phospholipid is preferably added at a rate of 100 to 3000 ml / min.
After completing the addition of the phospholipid dispersion to the health food material dispersion, a high-speed rotational dispersion process is performed by mechanical stirring in the dispersion process.

[Distributed processing step]
This dispersion treatment can be performed, for example, at 4000 to 20000 rpm with a high-speed rotating disperser, and is preferably performed at 8000 to 13000 rpm.
The treatment time can be about 10 to 60 minutes, preferably 20 to 40 minutes.
The treatment temperature can be about 20 to 70 ° C, preferably 40 to 60 ° C.
The health food material and phospholipid suspension obtained by this dispersion treatment step can be used as a highly functional health food.

  In addition, this suspension can be used as a highly functional health food in a liquid state, and can be a liquid food such as a beverage. Moreover, this suspension can also be utilized for solid foods, such as a tablet, a granule, and a chewable tablet, by making it dry by spray-drying or freeze-drying. Furthermore, it can be prepared by a usual method in combination with a food material, food additive or the like according to the food. Moreover, it can also be used for uses such as health foods, functional foods, foods for specified health use, and foods for the sick according to the functionality of each health food material.

When the highly functional health food contains bittern, it is preferable to add bittern at the same time when the phospholipid dispersion is added to the health food material dispersion in the preliminary dispersion step.
The amount of bittern added may vary depending on the health food material used and the composition of the phospholipid, but for example, the weight ratio of bittern: phospholipid can be 1: 6 to 1: 1.

The method for producing a highly functional health food according to the present invention is not limited to the above examples. For example, the health food material and the phospholipid are simultaneously added to water and preliminarily dispersed by mechanical stirring. After that, it is possible to perform high-speed rotation dispersion processing.
However, it is more preferable to adjust the health food material and the phospholipid by separately predispersing the above-described methods because the physiological activity of each health food material extract is further enhanced. .

Although it does not specifically limit as water used when manufacturing the highly functional health food of this invention, For example, deionized water, city water, distilled water, electrolyzed water, etc. can be used.
In addition, examples of the high-speed rotation type disperser used in the present invention include high-speed rotation type dispersers widely used in various food fields such as a dissolver type, a homomixer type, and a comb-tooth type.
Furthermore, as the dispersion treatment in the dispersion treatment step, precise dispersion using a high-pressure homogenizer or an ultrasonic homogenizer can be performed. It is also preferable to use such precision dispersion in combination with dispersion by a high-speed rotating disperser.

The suspension of the highly functional health food according to the present invention varies depending on the health food material used and the composition of the phospholipid, but either a liposome, a gel-like compound, a polymer dispersion, or these forms Of these, one or two or more are mixed.
Various reports on the effectiveness of the liposome have been made. For example, it has been reported that the bioavailability of insulin at the time of oral administration is increased by encapsulating insulin, which was normally invalidated by oral administration, in liposomes (Takeuchi H et al. Pharam. Res. 13 , 896, 1996, Muramatsu K et al. Biol.
Bull. 19, 1055, 1996, Iwanaga K et al. J. Pharm. Sci. 88, 248, 1999, etc.). It has also been reported that the bioavailability is increased by encapsulating erythropoietin in liposomes (Maitani Y et al. J. Pharam. Sci. 85, 440, 1996, etc.).
From this, in the highly functional health food of the present invention, if liposomes are mixed, it can be expected that the physiological activity is further increased.

Here, the liposome itself is a known one, and there is a conventional method for its production. Various studies have already been conducted on the particle size and pH of liposomes. For example, as for particle size, Biochemica et Biophysica Acta, 557, 9 (1979), 601, 559 (1980), Journal of Pharmaceutical
Sciences, 71, 806 (1982). Examples of pH-related materials include JP-A-63-96193, JP-A-64-85920, and JP-A-4-9337.

However, these reports are all based on the general physical properties of liposomes, bioactive peptides not covered by the present invention (Japanese Patent Laid-Open No. 15-171262), and other special drug-containing liposomes (Takeuchi H et al. Pharam. Res. 13, 896, 1996, Muramatsu K et al. Biol.
Bull. 19, 1055, 1996, Iwanaga K et al. J. Pharm. Sci. 88, 248, 1999, Maitani Y et al. J.
Pharam. Sci. 85, 440, 1996, etc.), mainly vitamin C, potassium, saponin, charanthin, each health food used in the highly functional health food of the present invention. Terpenoids such as momordesine, adenine, citrulline, lucine, lucioside, alcohol-degrading enzyme (ADH), aldehyde-degrading enzyme (ALDH), pyran derivative verbenalol and its glycosides verbenaline, verbenalol, berbenine, and nobiletin Or tangoletin, luteolin, flavonoids such as luteolin glucoside and rutin, benzopyrans such as methyl lipariochromene A, rosmarinic acid, caffeic acid, chicory acid, chlorogenic acid, neochlorogenic acid, isochlorogenic acid and other polyphenols, Carotenes, L Steroids such as sterols, 1-DNJ, for Proxeronine, not described at all.

  Also, in terms of physiological activity, the above report has blood pressure stabilizing action, stomachic action, diuretic action, intestinal action, diuretic action of cat beard extract, blood pressure lowering action, and pine wig extract. Anti-inflammatory diuretic action, analgesic action, antibacterial action, anti-glycemic action of mulberry leaf extract, nourishing tonic, anti-blood pressure, anti-carcinogenic effect of shikushisha extract, improvement of insulin resistance, anti-blood sugar effect , Antihypertensive effect, whitening prevention effect of loofah extract, antihypertensive action, antioxidative action, neuralgia improvement, antipyretic action, sympathetic excitatory action, anticarcinogenic action of tankan extract , Blood glucose level inhibitory effect, antioxidative effect, antihypertensive effect of Hosovawadan extract, whitening effect, antioxidant effect, immunostimulatory effect, or noni extract has Not intended to disclose 瘍 cytostatic effect, not related to the high functional health food of the present invention.

In the above embodiment, as the health food material, the bitter melon extract, the cat beard extract, the pine wig extract, the mulberry leaf extract, the seek shark extract, the loofah extract, the button boo extract, the tankan extract, and Hoso Bawadan Either an extract or a noni extract can be used, but it is also preferable to combine two or more of these. This makes it possible to produce a highly functional health food having both physiological activities.
Similarly, either egg yolk phospholipid or soybean phospholipid can be used as a phospholipid, or a combination thereof can be used.

Examples of the present invention will be described below. However, the present invention is not limited to these examples.
Example 1 uses a tankan as a health food material. Example 2 uses mulberry as a health food material. Example 3 uses loofah which is a plant of the family Ruriaceae as a health food material. Examples 4 and 5 use a seek dish as a health food material. Examples 6 and 7 use noni as a health food material. In addition, the test examples are for evaluating the highly functional health food of each example, test example 1 is related to example 1, test example 2 is related to example 2, test example 3 is performed Regarding Example 3, Test Example 4 relates to Examples 4 and 5, and Test Example 5 relates to Examples 6 and 7.

Example 1
As a tankan extract, 200 g of tankan produced in Okinawa Prefecture was cut into pieces, extracted with a mixed solvent of 600 ml of city water and 1400 ml of ethanol at 80 ° C. for 1 hour, the extraction residue was removed, and the resulting extract was frozen. What was adjusted by processing with a dryer was used.

Then, 3 g of Tancan extract powder, 7 g of phospholipid derived from soybean (SLP-PC70, manufactured by Tsuru-Lecithin Industry Co., Ltd.), and 300 ml of city water were placed in a 500 ml tall beaker, and a high-speed rotating disperser (TK). Homomixer MARK II fmodel Tokushu Kika Kogyo Co., Ltd. The same applies hereinafter.) For 5 minutes at room temperature and atmospheric pressure (open) at a rotation speed of 1500 rpm.
Thereafter, the rotational speed of the high-speed rotary disperser was set to 13000 rpm, and the system was operated at 60 ° C. for 30 minutes. This treated product was designated as Sample 1.

(Example 2)
As a mulberry leaf extract, 2000 g of Okinawa mulberry leaves were dried at 45 ° C. for 12 hours, and then dried mulberry leaves were cut into 1 cm square using a food chopper. After 200 g of the dried mulberry leaves were extracted with 2000 ml of city water at 90 ° C. for 1 hour, the extracted extract was removed and the extract obtained by treating with a freeze dryer was used. .

Then, 1 g of mulberry leaf extract powder, 7 g of phospholipid derived from soybean used in Example 1 and 300 ml of city water were placed in a 500 ml tall beaker, and at room temperature and atmospheric pressure (open) with a high-speed rotating disperser. ), Pre-dispersed for 5 minutes at a rotation speed of 1500 rpm.
Thereafter, the rotational speed of the high-speed rotary disperser was set to 13000 rpm, and the system was operated at 60 ° C. for 30 minutes.
This treated product was designated as Sample 2.

(Example 3)
As the loofah extract, 5 kg of loofah from Okinawa is first cooled to about 5 ° C. Next, the cooled loofah is cut with a cutter or the like, put into a home mixer, 1000 ml of cold water at around 5 ° C. is added, and pulverized for 2 to 3 minutes at full rotation. Subsequently, the crushed loofah is centrifuged at 8000 rpm for 20 minutes using a centrifuge. And only a supernatant liquid is taken out and a solid part is removed. Sodium hydroxide (NaOH) is added to the supernatant obtained by solid-liquid separation to adjust the supernatant within the range of pH 6.9 to pH 7.1, followed by filtration under reduced pressure. In addition, an extract obtained by treating the extract obtained from the loofah extract with a freeze dryer was used.

  Then, 3 g of loofah extract powder, 6 g of phospholipid derived from soybean used in Example 1, and 300 ml of city water were placed in a 500 ml tall beaker, and were heated at room temperature and atmospheric pressure (open) with a high-speed rotating disperser. Pre-dispersion was carried out at a rotation speed of 1500 rpm for 5 minutes.

Thereafter, the rotational speed of the high-speed rotary disperser was set to 13000 rpm, and the system was operated at 60 ° C. for 30 minutes.
This treated product was designated as Sample 3. Further, the sample 3 was divided in half, and one of them was subjected to ultrasonic dispersion treatment (ultrasonic treatment (UD-201 TOMY Seiko Co., Ltd., hereinafter the same)) at 60 ° C. under atmospheric pressure (open) for 30 minutes. The treated product was designated as sample 4.

Example 4
As the Shikushasha extract, 200 g of Okinawa Shikushasha was cut into pieces, extracted with a mixed solvent of 600 ml of city water and 1400 ml of ethanol at 80 ° C. for 1 hour, and then extracted residue was obtained. What was adjusted by processing an extract with a freeze dryer was used.

Then, 3 g of Shikshasha extract powder, 7 g of the phospholipid derived from soybean used in Example 1 and 300 ml of city water were placed in a 500 ml tall beaker, and at room temperature and atmospheric pressure (open) with a high-speed rotating disperser. ), Pre-dispersed for 5 minutes at a rotation speed of 1500 rpm.
Thereafter, the rotational speed of the high-speed rotary disperser was set to 13000 rpm, and the system was operated at 60 ° C. for 30 minutes.
This treated product was designated as Sample 5. Further, the sample 5 was divided into halves, and one of them was subjected to ultrasonic dispersion treatment at 60 ° C. and atmospheric pressure (open) for 30 minutes using an ultrasonic treatment machine.

(Example 5)
As a seeker extract, an extract prepared in the same manner as in Example 4 was used.
Then, 3 g of Shikshasha extract powder and 200 ml of city water are placed in a 500 ml tall beaker and pre-dispersed for 5 minutes at room temperature and atmospheric pressure (open) at a rotation speed of 1500 rpm with a high-speed rotating disperser. Was designated as A liquid.
In addition, 7 g of soybean-derived phospholipid used in Example 1 and 100 ml of city water were placed in a 300 ml beaker and subjected to a predispersion treatment under the same conditions as above, and this was designated as B solution.

Next, the liquid A was gradually added over 1 minute while stirring the prepared liquid A with a high-speed rotating disperser at normal temperature and atmospheric pressure (open) at a rotation speed of 1500 rpm.
After all the liquid B was added, the rotational speed of the high-speed rotary disperser was set to 13000 rpm, and the system was operated at 60 ° C for 30 minutes.
This treated product was designated as Sample 7. Further, the sample 7 was divided in half, and one of the samples was subjected to ultrasonic dispersion treatment at 60 ° C. and atmospheric pressure (open) for 30 minutes with an ultrasonic treatment machine.

(Example 6)
As noni extract, after slicing noni fruit from Okinawa, it is squeezed by applying a pressure of about 350 kg / cm2 using a fruit press, and the resulting juice is processed with a freeze dryer. The adjusted one was used.

  Then, 8 g of noni extract powder, 400 g of soybean-derived phospholipid used in Example 1, and 1800 ml of city water were placed in a 10,000 ml stainless steel beaker, and the mixture was heated at room temperature and atmospheric pressure (open) with a high-speed rotating disperser. It was operated at 60 ° C. for 30 minutes at a rotational speed of 13,000 rpm. This processed product was designated as Sample 9.

  A processed product similar to that of Sample 9 was further subjected to ultrasonic dispersion treatment at 60 ° C. under atmospheric pressure (open) for 30 minutes using an ultrasonic treatment machine.

A treated product similar to that of Sample 9 was further subjected to a dispersion treatment using an ultrahigh pressure homogenizer (microfluidizer), and this treated product was designated as Sample 11.
The operating conditions of the ultra-high pressure homogenizer are as follows. The pressure was 100 MPa, one pass. The input temperature and extraction temperature in the treatment were 35 ° C.

(Example 7)
As the noni extract, one prepared in the same manner as in Example 6 was used.
Then, 8 g of noni extract powder, 400 g of soybean-derived phospholipid used in Example 1, 1.44 g of bittern (from Tarama Island, Okinawa Prefecture) and 1800 ml of city water were placed in a 10,000 ml stainless steel beaker, and a high-speed rotating disperser Was operated at 60 ° C. for 30 minutes at room temperature and atmospheric pressure (open) at a rotational speed of 13000 rpm. This processed product was designated as Sample 12.

  A processed product similar to that of Sample 12 was further subjected to ultrasonic dispersion treatment at 60 ° C. under atmospheric pressure (open) for 30 minutes using an ultrasonic processor, and this processed product was designated as Sample 13.

A treated product similar to that of sample 12 was further subjected to a dispersion treatment using an ultra-high pressure homogenizer (microfluidizer).
The operating conditions of the ultra-high pressure homogenizer are as follows. The pressure was 100 MPa, one pass. The input temperature and extraction temperature in the treatment were 35 ° C.

(Test Example 1)
<Cancer growth inhibition test>
The highly functional health food (tancan extract, sample 1) obtained in Example 1 is administered once to normal cells and cancer cells, and then the amount of ATP produced by each cell is determined according to the following method. By examining, the cancer cell proliferation inhibitory effect by the highly functional health food of the present invention was evaluated.

(1) Test method First, a total of 3 groups for administering the following test substances were set as test groups.
-Test group I tankan extract-Test group II Mixture of tankan extract and soybean phospholipid (untreated composition with the same components and mixing ratio as in Example 1 but not mechanically processed)
・ Test group III sample 1 (tancan extract high-speed rotation processing)

(I) Preparation of sample solution Next, 50 mg of the above test substance was dissolved in 1 ml of phosphate buffered saline (pH-7.4) and sterilized by filtration with a 0.22 μM filtration filter (Millipore). This was used as a 1-fold solution, and this was serially diluted as necessary to prepare a sample. FIG. 1 shows the composition of the test group and the contents of the administered substance.

(Ii) Preparation of cells Leukocyte fraction prepared from human acute lymphocytic leukemia cell (T cell-derived) strain Molt-4, RPMI8402 and peripheral blood of healthy individuals using Lymphosepar I (Immuno-Biological Laboratories) Minutes were used as normal cells.

(2) Evaluation of cancer cell growth inhibitory action Each cell suspension (90 μl) and sample solution (10 μl) were added to a 96-well microplate so that the cell density was 4.5 × 10 ⁵ cells / ml. did.
Then, after culturing this at 37 ° C. for 24 hours in a 5% CO ₂ atmosphere, the amount of ATP produced by the cells was measured using an ATP measurement kit (Cell-Titer-Glo ^™ Luminescent Cell Viability Assay (Promega)). Measured.
Moreover, what added the same quantity phosphate buffered saline without adding the foodstuff material of this invention was made into the control | contrast.

The measurement result of the amount of ATP produced for RPMI8402, Molt-4 and normal cells is shown in FIG.
In the figure, the added concentration is the concentration (mg / ml) of the added sample solution, and the numerical value in the lower column of the cell name used in the test is the relative ATP when the ATP production of the control is 100. Indicates the production rate (%). When the value of this ATP production rate is less than 100, it means that cell growth is suppressed, and conversely, when it exceeds 100, it means that cells are activated.

FIG. 2 shows that test group I containing only the tankan extract does not show toxicity against normal cells, and tends to suppress cell growth against RPMI8402 and Molt-4, which are leukemia cells.
Moreover, the only test group II was mixed phospholipids Tankan extract test group I (Tankan extract only) revealed that almost the same effect on the cell.

However, Test Group III, in which a tankan extract and soybean phospholipid were dispersed by mechanical treatment, showed a very strong inhibition of cell growth compared to Test Groups I and II.
That is, the highly functional health food of the present invention containing the tankan extract produced in Example 1 is not toxic to normal cells and is 1 for leukemia cells RPMI8402 and Molt-4. Even in the case of an addition amount of .25, the cancer cell proliferation inhibitory effect with a survival rate of 50% or less is shown.

Therefore, according to the highly functional health food of the present invention in which a mixture of a tankan extract and soybean phospholipid is dispersed by mechanical treatment, it has been clarified that the cancer cell proliferation inhibitory effect is manifested in a smaller amount than before. It was.
On the other hand, it was clarified that the enhancement of the original cancer cell growth inhibitory effect cannot be expected only by mixing the tankan extract and soybean phospholipid without performing mechanical dispersion treatment.

(Test Example 2)
<Glucose level rise suppression test>
The high-functional health food (mulberry leaf extract, sample 2) obtained in Example 2 is administered once to an experimental animal, and then the change in blood glucose level of the mouse is examined according to the following method. Thus, the blood glucose level increase inhibitory effect of the highly functional health food of the present invention was evaluated.

(1) Test method First, a total of 6 groups for administering the following test substances were set as test groups.
Test group I Mulberry leaf extract (mulberry leaf extract solid content 25 mg / kg)
Test group II Mulberry leaf extract (mulberry leaf extract solid content 50 mg / kg)
Test group III Mulberry leaf extract and soybean phospholipid mixture (untreated composition with the same components and mixing ratio as in Example 1 but not mechanically treated) (mulberry leaf extract solids 25 mg / Kg)
Test group IV A mixture of mulberry leaf extract and soybean phospholipid (untreated composition with the same components and mixing ratio as in Example 1 and not subjected to mechanical treatment) (solid amount of mulberry leaf extract 50 mg / Kg)
Test group V Sample 2 (mulberry leaf extract high-speed rotation treatment ) (mulberry leaf extract solid content 25 mg / kg)
Test group VI Sample 2 (Mulberry leaf extract high-speed rotation treatment ) (Mulberry leaf extract solid content 50 mg / kg)

And about these test groups I-VI and a total of 7 groups of a control group, it tested using the mouse | mouth (ICR mouse | mouth, a male, 5 weeks old, n = 10, an acquisition place: Japan Charles River company).
After the control group and the test group were fasted for 16 hours, the mice in the test groups I to VI were used with a 1 ml syringe for tuberclee and an oral sonde for mice. The oral administration was forcibly adjusted to 25 mg / kg and 50 mg / kg.

A control group of mice was administered city water as described above. FIG. 3 shows the group structure of the test group and the contents of the administered substance.
Thirty minutes after administration, glucose and soluble starch were administered in the same manner as the mulberry leaf extract so as to give 3.3 g per kg of body weight.

The blood glucose level was measured at a total of 4 points before administration of the samples in the test group and the control group (0 minutes), 30 minutes, 60 minutes, and 120 minutes after administration of the samples in the test group and the control group.
The blood glucose level is measured by placing the mouse in a fixator, cutting the tail vein with a 25G syringe and bleeding, and measuring the blood glucose level using Glutest Ace G1640 (manufactured by Sanwa Chemical Laboratory Co., Ltd.). It was.

  After measuring the blood glucose level, the rate of increase in blood glucose level was calculated from the following equation, and the effect of suppressing the increase in postprandial blood glucose level of each sample was evaluated.

(Rate of increase in blood glucose level after meals)
Increase rate of blood glucose level (%) = (blood glucose level after glucose load / blood glucose level before glucose load) × 100-100

(2) Determination of effect Using the average value of the blood glucose level increase rate calculated as described above, a t-test was performed between the control group and each test group to determine the postprandial blood glucose level increase inhibitory effect. . The results are shown in FIGS. In each figure, the asterisk shown 30 minutes after the meal in the graph of increased blood glucose level is significant with one significant difference p <0.05 and two significant differences p <0.01. It means that.

Figure 4 is a diagram showing changes in the blood glucose level increase rate in glucose tolerance, FIG. 5 is a diagram showing a change in blood glucose level increase rate in soluble starch load.
In FIG. 4, the mulberry leaf extract control group I and the test group III that was not subjected to mechanical treatment (each mulberry leaf extract solid dose equivalent to 25 mg / kg administration) were compared with the mulberry leaf extract control. Although there was a tendency for the blood glucose level to increase with respect to the group, no significant difference was observed with respect to the control group.

On the other hand, the test group V in which mulberry leaf extract and soybean phospholipid were dispersed by mechanical treatment (administered in an amount corresponding to a solid amount of mulberry leaf extract 25 mg / kg) was significantly different from the control group (p <0). .05) was confirmed.
Furthermore, test group II with mulberry leaf extract alone and test group IV without mechanical treatment (administered in a solid amount of mulberry leaf extract 50 mg / kg, respectively) were significantly different from the control group ( p <0.05) was confirmed, but for Test Group VI (mulberry leaf extract solid content equivalent to 50 mg / kg administered) in which mulberry leaf extract and soybean phospholipid were dispersed by mechanical treatment A significant difference (p <0.01) was confirmed with respect to the control group.

Next, as shown in FIG. 5, the same results as in the case of using glucose were obtained for tests using soluble starch as the sugar to be loaded.
That is, for test group I with mulberry leaf extract only and test group III with no mechanical treatment (solid dose of mulberry leaf extract 25 mg / kg, respectively), mulberry leaf extract control group On the other hand, although there was a tendency for the rate of increase in blood glucose to decrease, none of the results showed a significant difference from the control group.

On the other hand, the test group V in which mulberry leaf extract and soybean phospholipid were dispersed by mechanical treatment (administered in an amount corresponding to a solid amount of mulberry leaf extract 25 mg / kg) was significantly different from the control group (p <0). .05) was confirmed.
Furthermore, test group II with mulberry leaf extract alone and test group IV without mechanical treatment (administered in an amount corresponding to a solid amount of mulberry leaf extract of 50 mg / kg, respectively) were significantly different from the control group ( p <0.05) was confirmed, but for Test Group VI (mulberry leaf extract solid content equivalent to 50 mg / kg administered) in which mulberry leaf extract and soybean phospholipid were dispersed by mechanical treatment A significant difference (p <0.01) was confirmed with respect to the control group.

From the above results, it has been clarified that the effect of suppressing the increase in blood glucose level is exhibited even when the amount of the test substance is small by performing the mechanical treatment in the present invention.
That is, in Test Example 2, the mixture of mulberry leaf extract and soybean phospholipid that had not been subjected to mechanical treatment showed a significant difference (p <0.05) from the control group for the first time at 50 mg / kg / dose. It is shown, and the blood glucose level increase inhibitory effect is not shown at doses lower than that.
On the other hand, the highly functional health food of the present invention obtained by dispersing mulberry leaf extract and soybean phospholipid by mechanical treatment shows an effect of suppressing an increase in blood glucose level at a dose of 25 mg / kg / dose. It was revealed that the effect of suppressing an increase in blood glucose level was manifested in a smaller amount than before.

Further, the highly functional health food of the present invention obtained by dispersing mulberry leaf extract and soybean phospholipid by mechanical treatment is administered at a dose of 50 mg / kg / dose that exhibits a conventional blood glucose level increase inhibitory effect. Thus, it was clarified that an even higher blood glucose level increase suppressing effect can be expected.
On the other hand, it is clear that if the mulberry leaf extract and soybean phospholipids are mixed and mechanical treatment is not applied, it is not expected to enhance the effect of suppressing the increase in blood glucose level without increasing the intake. It was.

(Test Example 3)
<Blood alcohol degradation acceleration test>
The high-functional health food obtained in Example 3 (loofah extract, samples 3 and 4) is administered stepwise to experimental animals, and then the effect on the concentration of alcohol in the blood of mice is examined according to the following method. Thus, the blood alcohol degradation promoting effect of the highly functional health food of the present invention was evaluated.

(1) Test method First, a total of 4 groups for administering the following test substances were set as test groups.
Test group I Loofah extract Extract of test group II Loofah extract and soybean phospholipid (untreated composition with the same components and mixing ratio as Example 1 but not mechanically treated)
・ Test group III sample 3 (high-speed rotation processing of loofah extract)
・ Test group IV sample 4 (high-speed spinach extract + ultrasonic treatment)

And about these test groups I-IV and a total of 5 groups of a control group, it tested using the mouse | mouth (ICR mouse | mouth, a male, 4 weeks old, n = 10, a source: Nippon Charles River company).
The mice of Test Groups I to IV were orally forcibly administered with each test substance using a 1 ml syringe for tuberclee and an oral sonde for mice so that the loofah extract was 1000 mg / kg in solid amount. A control group of mice was administered city water as described above. FIG. 6 shows the group structure of the test group and the contents of the administered substance.
Thirty minutes after the administration, 20% ethanol was administered in the same manner as the loofah extract so as to obtain 1.6 g / kg body weight.

After administration of the sample, blood was collected 30 minutes later, and the obtained serum was used as a measurement sample.
The alcohol concentration in blood was determined by measuring the alcohol concentration in plasma obtained from each test group and control group using a commercially available measurement kit (F kit ethanol JK International).

(2) Determination of effect Using the average value of blood alcohol concentration measured as described above, a t-test was performed between the control group and each test group to determine the effect of promoting blood alcohol degradation. . The result is shown in FIG. In the figure, the asterisk shown on the upper side of the bar graph of blood alcohol concentration in the test group is significant difference p <0.05 for one, significant difference p <0.01 for two, and the significant difference is It means that it is recognized.

In the same figure, the test group I with only the loofah extract and the test group II not subjected to the mechanical treatment of the present invention showed a tendency to decrease the blood alcohol concentration relative to the control group. There was no significant difference.
In addition, in the mechanical treatment of the present invention, a significant difference (p <0.05) was confirmed with respect to the control group for the test group III in which the loofah extract and soybean phospholipid were dispersed. For the test group IV, a significant difference (p <0.01) was confirmed with respect to the control group.

From the above results, it was clarified that the blood alcohol degradation promoting effect was exhibited only by performing the mechanical treatment of the present invention.
That is, in Test Example 3, the loofah extract used is 1000 mg / kg / dose, which is lower than the conventional effective amount, and therefore contains only this health food material (test groups I and II). ) Does not show an effective blood alcohol degradation promoting effect.
However, the highly functional health food of the present invention containing the loofah extract produced in Example 3 shows a blood alcohol degradation promoting effect at a dose of 1000 mg / kg / dose, in a smaller amount than before, It was clarified that blood alcohol degradation was promoted.

Furthermore, in the mechanical treatment of the present invention, it has been clarified that not only high-speed rotational dispersion treatment but also ultrasonic dispersion treatment can be expected to have a higher blood alcohol degradation promoting effect.
However, it has been clarified that the effect of promoting the promotion of alcohol degradation in blood cannot be expected only by mixing the loofah extract and soybean phospholipid without performing the mechanical treatment of the present invention.

(Test Example 4)
<Blood pressure increase suppression test>
The high-functional health foods obtained in Examples 4 and 5 (Shikshasha extract, samples 5 to 8) were continuously administered to experimental animals, and then the effects of systolic blood pressure in rats that developed hypertension were as follows. By examining according to the method, the blood pressure increase inhibitory effect of the highly functional health food of the present invention was evaluated.

(1) Test method First, a total of 6 groups for administering the following test substances were set as test groups.
-Test group I Shikushishasha extract-Testgroup II Mixture of Shikshasha extract and soybean phospholipid (untreated composition with the same components and mixing ratio as in Example 2 but not mechanically treated)
・ Test Group III Sample 5 (High-speed rotation processing of squeak shear extract / preliminary dispersion of materials simultaneously)
・ Test Group IV Sample 6 (High-speed rotation processing of squeezer extract + sonication / preliminary dispersion of materials simultaneously)
・ Test group V sample 7 (seek-shasher extract high-speed rotation treatment / preliminary dispersion of materials separately)
・ Test Group VI Sample 8 (High speed rotation of squeezer extract + sonication / preliminary dispersion of materials separately)

And about these test groups I-VI and a total of 7 groups of a control group, the test was done using the hypertensive rat (SHR, male, 8 weeks old, n = 10, a source: Charles River Japan).
Each test substance was forcibly orally administered to the rats of test groups I to VI using a 1 ml syringe for tuberclee and an oral sonde for rats so that the squeak shaker extract had a solid content of 250 mg / kg. .
The administration time was in the morning and the administration frequency was once a day. The administration period was 6 weeks.
A control group of mice was administered city water as described above. FIG. 8 shows the composition of the test group and the contents of the administered substance.

  Systolic blood pressure was measured once a week during the administration period. Systolic blood pressure was measured 5 times consecutively using the tail cuff method (Softron non-invasive automatic blood pressure measuring device, BP-98A Softlon, Tokyo), and 3 times excluding the highest and lowest blood pressure. Mean blood pressure was used.

(2) Determination of effect Using the average value of the systolic blood pressure measured as described above, a t-test was performed between the control group and each test group to determine the blood pressure increase suppressing effect. The result is shown in FIG. In the figure, the asterisk shown on the right side of the systolic blood pressure value in the test group is significantly different with one significant difference p <0.05 and two significant differences p <0.01. It means that.

In the same figure, in the test groups I and II that were not subjected to the mechanical treatment of the present invention, no significant difference was confirmed with respect to the control group.
In addition, in the mechanical treatment of the present invention, the test groups III and IV in which the sikh-shah extract and soybean phospholipid were simultaneously preliminarily dispersed were significantly different from the control group (p <0. 05) was confirmed, and it was confirmed that the systolic blood pressure was decreased.
In addition, in the mechanical treatment of the present invention, a significant difference (p <0.01) was confirmed with respect to the control group for the test group V in which the sikh-shah extract and soybean phospholipid were separately preliminarily dispersed. A significant difference (p <0.01) was also confirmed for the test group VI subjected to the sonic dispersion treatment with respect to the control group, but the systolic blood pressure value was further decreased as compared with the test group V.

From the above results, it was clarified that the blood pressure increase suppressing effect was manifested only by performing the mechanical treatment of the present invention.
In other words, in this Test Example 4, the used shikshisha extract is 250 mg / kg / day, which is less than the effective amount that expresses the original functionality, and therefore simply contains this health food material. The ones (test groups I and II) do not show an effective antihypertensive effect .
However, the highly functional health food of the present invention containing the squeezer extract produced in Examples 4 and 5 shows a blood pressure increase inhibitory effect at a dose of 250 mg / kg / day, which is higher than in the past. It was clarified that the effect of suppressing the increase in blood pressure was expressed with a small amount.

In addition, in the mechanical treatment of the present invention, it is clear that a higher blood pressure elevation suppressing effect can be expected by adjusting separately by pre-dispersing separately than by simultaneously pre-dispersing sikhshire extract and soybean phospholipid. became.
Furthermore, in the mechanical treatment according to the present invention, it has been clarified that a higher blood pressure rise suppressing effect can be expected by performing not only the high-speed rotational dispersion treatment but also the ultrasonic dispersion treatment.

(Test Example 5)
<Tumor cell growth inhibition test>
The high-functional health food obtained in Examples 6 and 7 (containing noni extract, samples 9 to 14) was administered to cancer-bearing animals, and changes in tumor volume were examined according to the following method, whereby high The tumor cell growth inhibitory effect in functional health food was evaluated.

(1) Test Method First, as a test group (test substance administration group), a total of 8 groups for administering the following test substances were set.
Test Group I Noni Extract Test Group II Noni Extract and Soy Phospholipid Mixture (Untreated Composition with the Same Components and Mixing Ratio as Example 4 but not Mechanically Treated)
・ Test group III sample 9 (noni extract high-speed rotation treatment / no bittern)
Test group IV sample 10 (noni extract high-speed rotation treatment + sonication / no bittern)
・ Test group V sample 11 (noni extract high-speed rotation treatment + ultra-high pressure homogenizer treatment / no bittern)
・ Test group VI Sample 12 (noni extract high-speed rotation treatment / with bittern)
Test group VII sample 13 (noni extract high-speed rotation treatment + ultrasonic treatment / with bittern)
Test group VIII sample 14 (noni extract high-speed rotation treatment + ultra-high pressure homogenizer treatment / with bittern)

And about these test groups I-VIII and a total of nine groups of a control group (control substance administration group), a mouse (nude mouse, BALB / c-nu, male, 4 weeks old, n = 10, acquisition place: Japan) (Charles River Co.) was prepared and inoculated with tumor cells, and these mice were bred with each substance administered, and changes in the tumor volume were examined.
Tumor cell inoculation was performed subcutaneously on the back using 100 μl of a physiological saline dispersion (cell number: 1 × 10 ⁶ cells / ml) of the mouse-derived tumor cell line Sarcoma180 using an injection needle 25G and a 1 ml syringe for tuberclee.

After inoculation, each test group was forcibly orally administered to each test group using a tuberclee 1 ml syringe and an oral sonde for mice so that the noni extract was 100 mg / kg in solid content.
As for the administration time, administration on the day with tumor measurement was performed in the afternoon after the end of tumor measurement, and administration on the day without tumor measurement was performed at approximately the same time. The frequency of administration was once a day. The administration period was 21 days.
A control group of mice was administered city water as described above. FIG. 10 shows the composition of the test group and the contents of the administered substance.

The tumor volume (mm ³ ) of each mouse was determined by measuring the major axis (a), minor axis (b), and height (c) of the tumor in mm with a caliper, and (a) × (b) × ( c) It was obtained by the equation of / 2.
The measurement points were 3 points on the 5th, 10th and 15th after administration of the test substance, and the measurement was performed once a day.

(2) Judgment of effect On the fifth, tenth, and fifteenth days after administration of the test substance, the average value of the estimated tumor volume of each administration group was calculated, and this value was used between the control group and each test group. The t-test was carried out to determine the tumor cell growth inhibitory effect. FIG. 11 is a graph showing changes in estimated tumor volume in each test group.

In the same figure, in the test groups I and II that were not subjected to the mechanical treatment of the present invention, no significant difference was confirmed with respect to the control group.
In addition, regarding the test group III in which the noni extract and soybean phospholipid were dispersed by the mechanical treatment of the present invention, according to the t test, the proliferation of the tumor cells was obvious, although not significant compared to the control group. It showed a downward trend.

In addition, in the mechanical treatment of the present invention, test group IV in which noni extract and soybean phospholipid were further subjected to ultrasonic dispersion treatment, test group in which noni extract and soybean phospholipid were further subjected to ultrahigh pressure homogenizer treatment For V, significant differences (p <0.05) and (p <0.01) were confirmed on the 15th day.
Furthermore, in the test groups VI, VII, and VIII in which the noni extract and soybean phospholipid were dispersed by the mechanical treatment of the present invention and the bittern was further added, each of the test groups VI, VII, and VIII (p A significant difference of <0.05), (p <0.01), and (p <0.001) was confirmed.

From the above results, it is considered that the highly functional health food produced in Examples 6 and 7 (samples 9 to 14: test groups III to VIII) can be expected to have a tumor growth inhibitory effect, and the mechanical treatment of the present invention. For the first time, it was revealed that the tumor cell growth inhibitory effect was expressed.
That is, in this Test Example 5, the noni extract used is 100 mg / kg / day, which is less than the effective amount that expresses the original functionality, and therefore simply contains this health food material ( Test groups I and II) do not show an effective tumor cell growth inhibitory effect.
However, the highly functional health food of the present invention containing the noni extract produced in Examples 6 and 7 shows a tumor cell growth inhibitory effect at a dose of 100 mg / kg / day, and is smaller than the conventional amount. It was revealed that the tumor cell growth inhibitory effect was expressed.

In particular, in the mechanical treatment of the present invention, it was revealed that a higher tumor growth inhibitory effect can be expected when noni extract and soybean phospholipid are subjected to not only high-speed rotational dispersion treatment but also ultrasonic dispersion treatment.
Further, in the mechanical treatment of the present invention, not only the high-speed rotational dispersion treatment but also the ultra-high pressure homogenizer treatment can be expected to have a higher tumor growth inhibitory effect than the ultrasonic dispersion treatment. It became clear.
Furthermore, it has been clarified that an even higher tumor cell growth inhibitory effect is expressed by adding bittern to the highly functional health food of the present invention.

  The present invention can be used for liquid foods such as beverages and solid foods such as tablets, granules, and chewable tablets. In addition, blood pressure stabilization action, healthy stomach action, diuretic action, bowel regulation action, diuretic action of Nekonohibo extract, antihypertensive action, anti-inflammatory diuretic action, analgesic action, antibacterial action, mulberry extract Blood sugar inhibitory effect, nourishing tonic, blood pressure inhibitory effect, shikushushisha extract cancer prevention effect, insulin resistance improvement, blood sugar level inhibitory effect, blood pressure inhibitory effect, hangover of loofah extract Prevention and whitening effect, antihypertensive action of anti-bacterial extract, antioxidant action, neuralgia improvement, antipyretic action, sympathomimetic action of tankan extract, carcinogenesis-inhibiting effect, blood glucose level-inhibiting effect, antioxidant effect, Hoso Bawadan Health according to blood pressure lowering action, whitening action, antioxidant action, immunostimulatory action, or tumor cell growth inhibitory effect of Noni extract Goods, functional foods, it is possible to use food for specified health use, in applications such as food for the sick.

It is a figure which shows the structure of the test group in Test example 1 of this invention, the compounding composition of an administration substance, and the presence or absence of the mechanical treatment of an administration substance. It is a figure which shows the measurement result of the amount of ATP production about RPMI8402, Molt-4, and a normal cell in Experiment 1 of this invention. It is a figure which shows the structure of the test group in Test example 2 of this invention, the compounding composition of an administration substance, and the presence or absence of the mechanical treatment of an administration substance. It is a figure which shows transition of the blood glucose level increase rate in the glucose load in Test example 2 of this invention. It is a figure which shows transition of the blood glucose level raise rate in the soluble starch load in Experiment 2 of this invention. It is a figure which shows the structure of the test group in Test example 3 of this invention, the compounding composition of an administration substance, and the presence or absence of the mechanical treatment of an administration substance. It is a figure which shows the blood alcohol concentration value in Test Example 3 of this invention. It is a figure which shows the structure of the test group in Test example 4 of this invention, the compounding composition of an administration substance, and the presence or absence of the mechanical treatment of an administration substance. It is a figure which shows the systolic blood pressure value in Test Example 4 of the present invention. It is a figure which shows the structure of the test group in Test example 5 of this invention, the compounding composition of an administration substance, and the presence or absence of the mechanical treatment of an administration substance. It is a graph showing transition of change in tumor volume in Test Example 5 of the present invention.

Claims (6)

A highly functional health food comprising a mixture of a health food material and a phospholipid derived from a natural product mechanically dispersed,
The health food material is a bitter melon extract, a black beard extract, an echidna extract, a mulberry leaf extract, a shikshasha extract, a loofah extract, a button bowfish extract, a tankan extract, a Hosobabadan extract, or a noni extract A highly functional health food, wherein the phospholipid derived from natural products is at least one selected from egg yolk phospholipid and soybean phospholipid.   The highly functional health food according to claim 1, wherein the mixture contains bittern.   The highly functional health food according to claim 1 or 2, wherein the mechanical dispersion treatment is at least one selected from high-speed rotation dispersion treatment, ultrasonic dispersion treatment, and high-pressure homogenizer dispersion treatment.   At least one selected from bitter melon extract, cat beard extract, pine pod extract, mulberry leaf extract, shikshusha extract, loofah extract, button bowfish extract, tankan extract, hosobabadan extract, or noni extract A high-function, characterized in that a health food material comprising the above and a natural product-derived phospholipid selected from egg yolk phospholipid or soybean phospholipid are mixed, and the resulting mixture is subjected to high-speed rotational dispersion treatment. Manufacturing method of sex health food.   The method for producing a highly functional health food according to claim 4, further comprising mixing bittern in the mixing.   6. The method for producing a highly functional health food according to claim 4, wherein the mixture is subjected to ultrasonic dispersion treatment or high-pressure homogenizer dispersion treatment after the high-speed rotation dispersion treatment.

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