201242583 六、發明說明： 【發明所屬之技術領域】 尤指一種適用於血 本發明係關於一種組織工程裝置 管相關研究之組織工程裝置。 【先前技術】 所謂的組織工程，即為-種利用生物活性物質，透過 體外培養或建構之方法，達到侈 — >设次再1ε·益g及組織之技 術。組織工程已發展二十餘车 .. 卞餘年，且目刚已有可能再造骨骼、 皮膚、肌腱、角膜等組織器官。 於組織或器官修復或再造的過程中，新的組織生長及 ^月需要適當的血管以傳送養分並移除廢物。目前已有研 九指出，多種血管生長因子具有幫助血管新生、組織修復、 再生的功能。 #此外，因先天畸形、外傷、感染 '腫瘤切除所造成之 :頭缺損，亦為組織工程所要面臨之問題之一。因此，目 前組織工程亦有關於對於不同血管組成在血管新生中扮寅 W色’以及結合不同的幹細胞及終端細胞並配合具骨路 誘導能力之骨膜等研究，以幫助修復骨頭缺損。 為了建立-個組織工程模型，以進行上述研究，目前 已發展可使用石夕質包覆動靜脈以做為促 工程裝置。如圖！所示，此組織工程裝置係包括一㈣^ Λ L g 1 1 _由將_㈣片捲曲並透過縫線⑴將石夕 “相對兩邊縫合所形成。當此組織工程裝置係裝設於一 201242583 待觀察主體獻時，該待觀察主體1〇之企管ι〇ι係穿雜 管11之中空孔洞112。若欲研究不同待測物對於血管新生的 影響，則可於中空孔洞112中施予待測物。 然而，此矽膠管1】之忐—、，— 11之兩缟亚非密封，故當將待測物放 於中空孔洞112中時，待測物可能會擴散至石夕膠管η外， 而影響到研究的進行。據此，目前需發展一種組織工程裝 置’、可另效用於研究各種待測物對於血管新生之影響。 【發明内容】 本發明之主要目的係在提供—種組織觀察裝置，俾能 疋仏空間以將#觀察之血管區域密封於此空間中。 為達成上述目的，本發明之組織觀察裝置係包括：一 一：半筒；一第二半筒’係與第-半筒相互結合而組設成 一:空筒體，中空筒體之第—端係具有一第一開口且中 空筒體之第二端係具有一第_ ^ . n 第—開口；一第一蓋體，係組設 於中空筒體之第一端上並對岸 _ τ匕甲二简體之第一開口，透過 第：盘體之開合以打開或關閉第—開口，I第—蓋體具有 i少一％ —驽1〖在此，係使用 ,此你便用，芏少一 ”表示，故已代表可 有兩個穿孔之情況。因此，為得到較大範面，建議仍不要 ^篇以句：以及—第二蓋體，餘設於中空筒體之第 -端上並對應中空筒體之第二開口，透過第二蓋 合 以打開或關閉第二開口 ，曰笛 . ^ 開 且第二蓋體具有至少一第二穿 '、中田組織工程裝置組設於—待觀察主體上時待 201242583 觀察主體之血管係依序穿過第一蓋體之第一穿孔、中空筒 體、以及第二蓋體之第二穿孔。 3 本發明之組織工程裝置，可提供一可使血管穿過之密 閉空間。因此’當本發明之組織工程裝置裝設於待觀察主 體上（如：老鼠）時，可依照觀察目的而於此組織工程裝置之 令空筒體内放置不同之待測物’如骨膜、氧氣、生長因子 等，不同的生物集合成的聚合物以研究各種待測物對血管 新生之影響。 於本發明之組織工程裝置中，第一蓋體之第—穿孔中 與第二蓋體之第二穿孔中係分別設有—氣球，且藉由氣球 =塌縮或賴’以調整第-f孔與第二穿孔之孔洞面積。 藉由設置氣球，可填補血管穿過之第一穿孔及第二穿孔之 剩餘面積，以提升組織玉程裝置之密封性。當組織工程裝 置之密封性提升時，則可應用於局部血管組織工程研究: 靜脈血循不足（venous congesti〇n)、動脈供血不足 insufficiency)等研究中。 於本發明之組織工程裝置中，調整第一穿孔與第二穿 孔之孔洞面積可透過下列兩種方式。其中一種方^可: 於第-蓋體及第二蓋體上可t設有—充氣閥m氣 閥’充氣閥係連接氣球以使氣球膨脹，而錢閥係連接氣 球以使氣球塌縮；而另外一種方式可為：組織工程裝置可 更包括-充氣裝置，係連接氣球，充氣裝置係輸入4體 之氣球中以使氣球膨脹。 201242583 此外，於本發明之組織工程裝置中，第一半筒係具有 第一接面’第二半筒係具有第二接面，且第一接面係與第 二接面相互對應。在此，第一接面與第二接面之結合方式 益無特殊限制’可使用本技術領域已知之方式結合，例如： 第一半筒之第一接面與第二半筒之第二接面可分別具有一 L型結構，且第一半筒與第二半筒之L型結構係相互嵌合； 第半向、第一接面係設有複數插孔，第二半筒之第二接 面係設有複數插針，插針係與插孔相互對應，且當第一半 筒與第二半筒組設成中空筒體時，插針係插鞘於插孔中； 第-半筒之第一接面與第二半筒之第二接面係分別設有一 磁鐵’且第-半筒與第三半筒係透過磁鐵相互結合；或者 第一半筒之外壁係設有一凸緣，第二半筒之外壁設有一扣 鉤件，且藉由扣鉤件與凸緣相互扣勾以將第一半筒與第二 半筒組設成中空筒體。此外’第一半筒之兩個第二接面與 第二半：之兩個第二接面’可以相同或相亦方式结合。 第 面可分別設有/密封 第一接面及/或第二半筒之第二接 層’以提升組織工程裝置之密封性 本發二加Γ一蓋體及第二蓋體與中空筒體之密合性， 以二：之、:且織工程裝置可更包括1件，纟中中空筒體之 第一端及第二端之管壁係分別設有〜 冰砂 厶π々堃Α 卓—結合孔，第〆結 &孔之弟一部係設於第一半筒上， ..^ tL 第—結合孔之第二部係 a又方、弟一+同上，且當第一蓋體及 萆—盍體絚設於中空同 另一方面，於本發明之組織工程裝置中%一半筒之 201242583 體時，鎖件係插入第一結合孔，以將第一蓋體及第二蓋體 固定組設於中空筒體上。 此外，為使第一蓋體及第二蓋體不致於從中空筒體上 掉落，中空筒體之第一端及第二端係分別設有一密合凹 槽，第一蓋體及第二蓋體係分別設有一密合部，且當第一 蓋體及第二蓋體組設於中空筒體時，密合部係卡置於密合 凹槽中。 此外，於本發明之組織工程裝置中，第一蓋體及第二 蓋體係分別設有一滚珠軸承。藉由此滚珠轴承之設置，可 固定第一蓋體及第二蓋體與血管之相對位置，而防止因待 觀察主體的運動而導致血管扭曲。 再者，於本發明之組織工程裝置中，中空筒體之第一 端及第二端可分別設有一斜面，其中斜面上係設有一保護 層，而保護層之材料可為石夕膠。透過此斜面可緩和管壁邊 緣之角度，而保護血管不致於因中空筒體管壁稜角而受到 損害。 另一方面，本發明之組織工程裝置可更包括一循環裝 置，該循環裝置係包括：一馬達，其包括一輸出部、以及 一汲取部；一第一固定元件，係組設於待觀察主體上；一 第二固定元件，係組設於待觀察主體上；一第一輸送管， 其兩端係分別與輸出部及第一固定元件連接；以及一第二 輸送管，其兩端係分別與汲取部與第二固定元件連接；其 中，中空筒體之管壁係具有一流體輸入口、及一流體輸出 口，且該中空筒體更包括一流體輸入管、及一流體輸出管， 201242583 士體：入：之兩端係分別與流體輸入口及與連接至第一固 管連接’流體輸出管之兩端係分別與流 至第-固定讀之第二輸送管連接，透過 二裳輪出流體及汲取部吸取流體，使流體係依序 =-：送管、流體輸入管、t空筒體、流體輸出管、 以及第二輸送管。藉此，可㈣液體或氣體交換之目的。 〃此夕、纟發明之組織工程裝置可更包括一第一内半 I及帛一内半同’第-内半筒及第二内半筒係相互結 5而組設成一中空内筒，且中空内筒係位於中空筒體内。 在=中工體及中空内筒之管壁可設有複數孔洞且中 空筒體及中空内筒所形成之空間係裝設有—滲透膜。較佳 為’此渗透膜係為一具有分子量選擇性之薄膜。透過此渗 透膜之設置’可調整傳送至中空内筒内之分子種類。 再者’本發明之組織工程裝置可更包括有複數滲透 膜’餘向固定於中空筒體内；或可更包括有複數隔板、 及複數㈣膜，隔板及㈣關徑向固定於該中空筒體 内藉由π透膜及/或隔板之設置，可分隔中空筒體之内部 空間。若於不同的内部处pq枚32 一 j ^。丨二間放置不同待測物，因滲透膜及/ ^隔板之孔/同選擇性’而可控制傳送於中空筒體内之分子 種類或大小。 另方面，本發明之組織工程裝置可更包括複數固定 兀件ίτ“又於中空同體上，i中空筒體係透過固定元件以 ^定於待觀察主體上。藉此，可固定組織工程裝置於待觀 察主體上之相對位置。 201242583 【實施方式】 以下係藉由特定的具體實施例說明本發明之實施方 式，熟習此技藝之人士可由本說明書所揭示之内容輕易地 了解本發明之其他優點與功效。本發明亦可藉由其他不同 的具體實施例加以施行或應用，本說明書中的各項細節亦 "T基於不同觀點與應用，在不恃離本發明之精神下進行各 種修飾與變更。 實施例1 圖2係本實施例之組織工程裝置裝設於待觀察主體上 之不意圖。其中，本實施例之組織工程裝置係包括：一第 —半筒21、一第二半筒22、一第一蓋體23、以及一第二蓋 體24。當本實施例之組織工程裝置組設於—待觀察主體！ 〇 上時’待觀察主體10之血管101係穿過第一蓋體23及第二蓋 體24上所設之穿孔（圖中未視）。 接著’將詳細說明本實施例之組織工程裝置之结構。 如圖3 A所示，本實施例之組織工程裝置係包括：一第一半 筒2 1 ; —第二半筒22 ’係與第一半筒2 1相互結合而組設成 一中空筒體25 ’中空筒體25之第一端係具有一第一開口 251，且中空筒體25之第二端係具有一第二開口 252 ; 一第 一蓋體23，係組設於中空筒體25之第一端上並對應於中空 筒體25之第一開口 25丨’透過第一蓋體23之開合以打開或關 閉第一開口 251 ’且第一蓋體23係具有至少一第一穿孔 23 1 ;以及一第二蓋體24 ’係組設於中空筒體25之第二端上 並對應於中空筒體25之第二開口 252，透過第二蓋體24之開 10 201242583 =打開或關閉第二開σ 252,且第二蓋體24係具有至少— 第一=孔241。其中，當組織工程裝置組設於—待觀察主體 ^寸（β同％冬閱圖2)，待觀察主體1〇之血管丨⑴係依序穿 過弟-盍體23之第-穿孔23丨、中空筒體25、以及第二蓋體 24之第二穿礼241。 於本實允例中，第一蓋體23係具有兩個第一穿孔231， 第，蓋體24係具有兩個第二穿孔241，且兩個第一穿孔231 及第二穿礼24丨係分別用以使動脈及靜脈通過。 因此，當第一蓋體23及第二蓋體24組設於中空筒體25 上夺可形成一岔閉空間，以進行組織工程研究及觀察血 官的情形。 此外，為了將第一蓋體23及第二蓋體24固定在中空筒 肢25_^不致於脫落，第一蓋體23之凸緣232上設有一密合部 233，第二蓋體24之凸緣242上亦設有一密合部243，且十空 同體25之内惻兩端係分別設有一密合凹槽253，而兩端之密 合凹槽253係分別對應於第一蓋體23之密合部233及第二蓋 體24密合部243。因此，透過將密合部233, 243卡置於密合 凹槽253中，可將第一蓋體23及第二蓋體24組設並固定於中 空筒體25兩端。 接著’將說明本實施例之第一半筒2丨與第二半筒22之 接合方式。於本實施例中，第一半筒21及第二半筒22之兩 接面係採用不同方式接合。如圖3Β所示，第一半筒2丨係具 有第一接面2丨1，第二半筒22係具有第二接面22][，且第一 接面2 1 1係與第二接面22 1相互對應。其中，第一半筒2 1之 201242583 其中之一第一接面2丨1與第二半筒22之其中之一第二接面 22 1係分別具有相互對應之[型結構，故當第一半筒2丨與第 二半筒22組設成中空筒體時，第一半筒2丨與第二半筒“係 藉由L型結構相互嵌合。 此外，於本實施例甲，第一半筒2 1之另一第一接面2 ! i 與第二半筒22之另一第二接面221之結合，可採用鉸鏈之方 式結合。如圖3C所示，第一半筒21之外壁及第二半筒22之 外壁係設有鉸鏈29。 因此，於本實施例中，第一半筒2丨及第二半筒22之接 面为別以L型結構及鉸鏈方式接合。然而，第一半筒21及第 一半筒22之接面亦可以相同方式接合以組設成中空筒體。 實施例2 圖4係為本貫施例之組織工程裝置之示意圖。本實施例 之組織工程裝置係與實施例1之組織工程裝置相似，除了第 一半筒與第二半筒結合方式、以及第一蓋體與第二蓋體結 構與實施例1不同。 如圖4所示，第一半筒21係具有第一接面2丨1，第二半 向22係具有第二接面221，且第一接面211係與第二接面221 相互對應。 於本實施例，第一半筒21之其中之—第一接面21丨與第 —半筒22之其中之一第二接面22 1係分別具有一 l型結構， 且當第一半筒21與第二半筒22組設成中空筒體時，第一半 筒21與第二半茼22係藉由L型結構相互嵌合。 201242583 另一方面’第一半筒2 1之另一第一接面2 1 1係設有複數 插孔2 111 ’第二半筒22之另一第二接面22丨係設有複數插針 22 1 1 ’第二接面22 1上之插針22 1係與第一接面2 1 1之插礼 2111相互對應，且當第一半筒21與第二半筒22組設成中空 筒體時，插針2211係插鞘於插孔2111中，如圖4及圖5(A)所 示。 此外’於第一半筒21之第一接面211上，更可設有一密 封層26卜亚透過此密封層26丨可使第一接面2丨丨與第二接面 2 2 1更佳密合。 於本實施例中，第一半筒2 1及第二半筒22係以兩種不 同方式（L型結構、及插針與插孔）接合以組設成中空筒體， 如圖4及圖5(A)所示。然而，第一半筒2丨及第二半筒22之接 面亦可以相同方式接合以組設成中空筒體。 另一方面，於本實施例之組織工程裝置，第一蓋體U 及第一蓋體24係分別設有一滾珠軸承244(在此，第一蓋體 23之滾珠軸承未示），其中滾珠軸承244係包括：一外環 244!、一内％2442、以及複數滾珠2443，滾珠2443係設於 外％ 244 1與内環2442間’且滾珠軸承244之外環μ ^係做為 一密合部，以卡置於密合凹槽⑸中。當本實施例之組織工 程裝置組設於-待觀察主體上時，此滾珠軸承⑽可固定第 穿孔231及第—穿孔241與血管之相對位置，防止因待觀 察主體掙扎造成中空筒體移動，而扭曲待觀察主體之血 官。此外，第-蓋體23係具有—個第一穿孔23丨，第二蓋體 201242583 24係具有一個第二穿孔24卜故本實施例之組織工裎裝置僅 可使一條血管通過。 除了使用L型結構、及插針與插孔等方式外，更可以下 述其他方式將第一半筒21及第二半筒22結合。 如圖5(B)所示，第一半筒2丨之第一接面2丨】與第二半筒 22之第二接面221係分別具有相互對應之圓弧型結構。 如圖5(C)所示’第一半筒21之第一接面211與第二半筒 22之第二接面22 1係分別設有一磁鐵21 1 2，22 12，且第一半 筒2 1與第二半筒22係透過磁鐵2 112, 22 1 2之磁力相互纟士人。 如圖5(D)所示，第一半筒21之外壁係設有一凸緣2n， 第二半筒22之外壁設有一扣鉤件223，且藉由扣鉤件223與 凸緣213相互扣勾以將第一半筒2丨與第二半筒22組設成該 中空筒體（圖中未示）。 實施例3 圖6係為本實施例之組織工程裝置之示意圖。本實施例 之組織工程裝置係與實施例1之組織工程裝置相似，除了第 半筒與第二半筒結合方式、以及第一蓋體與第二蓋體結 構與實施例1不同。在此，僅詳述組織工程裝置之第一蓋體 部分。 如圖ό所示’本實施例之組織工程裝置更包括一鎖件 27，其係設於第一蓋體23上。其中’中空筒體乃之第一端 之官壁係設有一第一結合礼254 ’第一結合孔254之第一部 212係設於第—半筒21上，第一結合孔254之第二部222係設 於第二半筒22上。因此’當第一蓋體23組設於中空筒體25 14 201242583 :’鎖件27係插入第一結合孔254，藉由旋轉鎖件27可將鎖 件27固設於第—結合孔254中，以將第-蓋體23固定电設於 中空筒體25上。藉由鎖件27卡置於第'结合孔2M中，可防 止第-半筒21及第二半筒22分離。於本實施例十，鎖件27 之卡制部27丨係對應第一結合孔254之形狀，且呈現〗型。 除此之外，本實施例之組織工程裝置，第一蓋體h係 具有一個第一穿孔231 ’第二蓋體（圖中未示）亦對應第一蓋 體23而具有一個第二穿孔(圖中未示)’故本實施例之組織工 知裝置僅可使一條血管通過。 於本實施例之組織工程裝置中，由於第一蓋體及第二 盍祖組ό又於中空筒體之方式相同，故在此僅說明第一蓋體 組設於中空筒體之第一端之情形。 實施例4 如圖7所示，此為本實施例之組織工程裝置之示意圖。 本實施例之組織工程裝置係與實施例3之組織工程裝置相 似’鎖件2 7之卡制部2 7 1係呈S型。 實施例5 圖8(A)係為本實施例之設有空間調節裝置之組織工程 裝置之局部示意圖。本實施例之組織工程裝置係與實施例2 之組織工程裝置相似，除了本實施例之組織工程裝置更設 有一空間調節裝置。 如圖8( Α)所示，於本實施例之組織工程裝置中，第— 蓋體23之第一穿孔23丨中係設有一氣球28。此外，第一蓋體 2 3上更設有一充氣閥2 8 1、及一沒氣閥2 8 2 ’其中充氣閥2 8 1 201242583 係透過一充氣管283連接至氣球28，以將氣體通入氣球28中 使氣球28膨脹；而洩氣閥282則透過一洩氣管284連接至氣 球28 ’多餘的氣體則藉由洩氣管284從氣球28中排出。 因此，於本實施例之組織工程裝置中，可藉由氣球28 之塌縮或膨脹’以調整第一穿孔23 1之孔洞面積。藉此，當 本實施例之組織工程裝置裝設於一待觀察主體上時，因氣 球2 8可調整第一穿孔2 3 1之孔洞面積’故可更加密合血管 1 0 1所穿過之第一穿孔23 1 ’而提升組織工程裝置之密封性。 於本實施例之組織工程裝置中，由於第一蓋體及第二 蓋體均設有相同的空間調整裝置，故在此僅說明組設於第 一蓋體上之空間調整裝置， 實施例6 圖8(B)係為本實施例之設有空間調節裝置之組織工程 裝置之局部示意圖。本實施例之組織工程裝置係與實施例5 之組織工程裝置相似，除了本實施例之空間調節裝置係包 括：一氣球、以及一充器裝置。 如圖8(B)所示’於本實施例之組織工程裝置中第一 蓋體23之第一穿孔231中係設有一氣球28。此外，本實施例 之組織工程裝置更包括一充氣裝置285，係透過一充氣管 283連接氣球28，且充氣裝置285係輸入—氣體至氣球“中 以使氣球28膨脹。此外，空間調節裝置更包括—氣壓計 286，其係與充氣裝置285連接，以調整輸入至氣球28中之 氣體流速、體積、或壓力。 201242583 於本貫鈀例之組織工裎裝置中，由於第一蓋體之第一 穿孔及第二盍體之第二穿孔岣設有相同的空間調整裝置， 故在此僅說明組設於第一蓋體上之空間調整裝置。此外， 设於第一穿孔及第二穿孔中之氣球，可使用同一個充器裝 置進行充氣。 實施例7 圖9係本實施例之結合有循環裝置之組織工程裝置之 示意圖。本實施例之組織工程裝置係與實施例2之組織工程 裝置相似，除了本實施例之組織工程裝置更設有一循環裝 置。 如圖9所不，於本實施例之組織工程裝置更包括一循環 裝置，其包括：一馬達30,其包括一輪出部3(H、以及一汲 取部302;-第-岐元件η，係組設於待觀察主體ι〇之皮 膚103上；一第二固定元件32,係組設於待觀察主體⑺之皮 膚103上：一第一輸送管3〇3,其兩端係分別與輸出部训及 第一固定元件3!連接；以及-第二輸送管3()4,其兩端係分 別與汲取部302與第二固定元件32連接。在此，第—固定元 件31可透過縫線3丨1或插針3〗2以固定於待觀察主體丨〇之皮 膚1〇3表面及組織丨〇2上；且第二固定元件u亦可以與第一 固定元件3丨相同之方式（縫線32丨或插針322)固定於待觀察 主體10上。 其中，中空筒體25之管壁係具有—流體輪入口 255、一 流體輸出σ 256’且中空筒體25更包括_流體輸人管W、 及-流體輸出管258。流體輸入管257之兩端係分別與流體 201242583 輸入口 255及連接至第一固定元件3i之第一輪送管連 接，流體輸出管258之兩端係分別與流體輸出口 258及連接 至第一固疋元件32之第_輸送管304連接。因此，於本實施 例中，透過馬達30之輸出部301輸出流體、及汲取部3〇2吸 取流體，可使流體係依序流經第一輸送管3〇3、流體輸入管 257、中空茼體25、流體輸出管258、以及第二輸送管304。 藉此’可達到氣體或液體交換之目的。 藉由设置此循環裝置，本實施例之組織工程裝置可用 於各種影響血管新生因子的研究中，如生長因子調控氧 氣/二氧化碳交換、超/低比重氧氣治療（hyper/hyp〇baHc oxygen therapy)等。此外，若於本實施例之組織工裎裝置上 加裝一加熱器及一負回饋溫度感應器，則可調控溫度或 用於採討溫度對於血管影響的研究中。 實施例8 圖丨0係本實施例之組織工程裝置之示意圖。本實施例 之組織工程裝置係與實施例2之組織工程裝置相似’除了本 實施例之組織工程裝置更設有一中空内筒。 如圖1 0所示，於本實施例之組織工程裝置更包括一第 内半筒215、及-第二内半筒225，第一内半筒及第二 内半同225係相互結合而組設成—中空内筒。於本實施例 中第内半琦215係對應於第一半筒21且設於第一半筒以 内側，而第二内半筒225係對應於第二半筒22且設於第二半 筒22内側。因此’當第_半筒2|及第二半筒22組設成中空 201242583 筒體，而第一内半筒2丨5及第二内半筒225組設成中空内筒 時，中空内筒係位於中空筒體内。 此外，於本實施例之組織工程裝置中，中空筒體（即第 一半筒21及第二半筒22)及中空内筒（即第一内半筒215及第 一内半筒225)之管壁係設有複數孔洞2[4，224，Μ。 225 1，且中空筒體及中空内筒所形成之空間係裝設有一滲 透膜40。此外，置於中空筒體及中空内筒間之滲透膜40係 可置換的。 實施例9 圖1 1係本實施例之組織工程裝置之示意圖。本實施例 之組織工程裝置係與實施例1之組織工程裝置相似，除了本 實施例之組織工程裝置更包括有複數滲透膜5〇，係徑向固 定於中空筒體25内。同時，滲透膜5〇亦具有第三穿孔5〇1， 以使待觀察主體之血管通過。 更詳細而言，本實施例之組織工程裝置，中空筒體25 内壁係設有固定凹槽259 ’而滲透膜5〇則徑向固定於固定凹 槽259中。當第一半筒21及第二半筒22組設成中空筒體時， 滲透膜50則可將中空筒體内部空間分隔成數個小空間。由 於/爹透膜5 0係為一種可讓具有特定大小分子經過之具分子 $選擇性薄膜，藉由在中空筒體所被分隔成之小空間中放 置不同物質’可達到選擇性的釋放物質之目的。 實施例10 圖1 2係本實施例之組織工程裝置之示意圖。本實施例 之組織工裎裝置係與實施例9之組織工程裝置相似，除了本 201242583 實施例之組織工程裝置更包括有複數隔板60，其與滲透膜 50以相同方式徑向固定於中空筒體25内。此外，隔板6〇更 具有第四穿孔601 ’以使待觀察主體之血管通過。再者，隔 板60更具有貫穿隔板6〇之複數貫穿孔6〇2。 如圖1 2所示’本實施例之組織工程裝置從第一蓋體23 或第二蓋體24之軸向方向係依序設置有滲透膜5〇及隔板 60，而可將中空筒體内部空間分隔成數個小空間。在此， 車乂佳係於滲透膜5 〇與隔板6〇所形成之空間中放置待研究物 質，而此待研究物質可藉由隔板6〇之第四穿孔6〇1傳送至兩 隔板60所形成之空間中，但由於使用具分子量選擇性之滲 透膜50而可防止待研究物質傳送至滲透膜5〇與第一蓋體23 或第二蓋體24所形成之空間中。 此外’為使滲透膜50及隔板6〇所分隔出之空間具有密 封性，可選擇性地於第三穿孔5〇 1及第四穿孔6〇 1内設置空 間調節裝置，如圖8(A)或圖8(B)所示。 實施例11 圖1 3 6本貫施例之組織工程裝置之中空筒體之第一端 之示意圖。本實施例之組織工程裝置係與實施例9之組織工 程裝置相似，除了本實施例之組織工程裝置之中空筒體之 第一端及第二端（圖中未示）係分別設有一斜面2511，此斜面 25丨1上設有一保護層70，且保護層70之材料為矽膠。 由於本實施例之組織工程裝置之中空筒體之第一端及 第二端設有斜面及保護層，故可保護血管不易受到中空筒 體邊緣稜角而損害。 201242583 實施例12 圖14係本實施例之組織工程裝置之示意圖。本實施例 之，·且織工轾裝置係與實施例2之組織工程裝置相似，除了本 ==之組織卫程裝置更包括複數固U件70,係設於令 二筒胆25上，且中空筒體25係透過固定元件7〇以固定於待 觀'τ'主體(圖中未不)上。更詳細而言，固定元件之一端係 。又於中空同體25之外側，而固定元件7〇之另一端係設有— 疋孔70丨’且藉由縫線（圖中未示）穿過固定孔丨，可將固 定το件70固定於待觀察主體（圖中未示）上。 實施例13 圖15係本實施例之組織工程裝置之示意圖。本實施例 之組織工程裝置係與實施例1之組織工裎裝置相似，除了本 賞施例之組織工程裝置之中空筒體係為方形且中空筒體 之底部係設有一隔板60。 因此，當本實施例之組織工程裝置裝設於待觀察主體 上時’隔板60上方空間係設置待研究物質隔㈣下方空 間則用以置放待觀察主體之血管，而待研究物質係透過隔 板60之貫穿孔6〇2通入置放有血管之空間中(即隔板 空間）。 上述實施例僅係為了方便說明而舉例而已本發明所 主張之權利範圍自應以申請專利範圍所述為準而非僅限 於上述實施例。 【圖式簡單說明】 201242583 圖1係習知之組織工程裝置。 圖2係本發明實施例丨之組織工程裝置裝設於待觀察主體上 之不意圖。 圖3A係本發明實施例丨之組織工程裝置之第—蓋體及第二 蓋體之分解示意圖。 圖3 B係本發明實施例1之組織工程裝置之第—半筒及第二 半筒之其中一側接面示意圖。 圖3C係本發明實施例1之組織工程裝置之第—半筒及第二 半筒之另一側接面示意圖。 圖4係本發明實施例2之組織工程裝置之示衰圖。 圖5係本發明之組織工程裝置之第一半筒及第二半筒結合 之不意圖。 圖6係本發明實施例3之組織工程裝置之第—蓋體組設於中 空筒體之示意圖。 圖7係本發明實施例4之組織卫程裝置之第―蓋體組設於中 空筒體之局部示意圖。。 圖8(AH系本發明實施例5之設有空間調節裝置之組織工程 裝置之局部示意圖。 圖_係本發明實施例6之設有空間調節裝置之組織工程 裝置之局部示意圖。 圖9係本發明實施例7之結合有循環裝置之組織工程裝置之 示意圖。 圖10係本發明實施例8之組織工程裝置之示咅圖。 圖1 1係本發明實施例9之組織工程裝置之示咅圖。 201242583 圖1 2係本發明實施例1 0之組織工程裝置之示意圖。 圖丨3係本發明實施例1 1之組織工程裝置之中空筒體之第一 端之示意圖。 圖1 4係本發明實施例1 2之組織工程裝置之示意圖。 圖15係本發明實施例13之組織工程裝置之示意圖。 * 【主要元件符號說明】 10 待觀察主體 101 血管 102 組織 103 皮膚 11 矽膠管 111 縫線 112 中空孔洞 21 第一半筒 21 1 第一接面 2111 插孔 2112, 2212 磁鐵 212 第一部 213 Λ緣 214, 224, 2151, 2251 孔洞 215 第一内半筒 22 第二半筒 221 第二接面 2211 插針 222 第二部 223 扣鉤件 225 第二内半筒 23 第一蓋體 23 1 第一穿孔 232 凸緣 233 密合部 24 第二蓋體 241 第二穿孔 242 凸緣 243 密合部 244 滚珠轴承 2441 外環 2442 内環 2443 滾珠 25 中空筒體 第一開口 第二開口 第一結合孔 流體輸出口 流體輸出管 密封層 卡制部 充氣間 充氣管 充氣裝置 鉸鍵 輸出部 第一輸送管 第一固定元件 插針 縫線 滲透膜 搞板 貫穿孔 固定子匕 2511 斜面 253 密合凹槽 255 流體輸入口 257 流體輸入管 259 固定凹槽 27 鎖件 28 氣球 282 茂氣閥 284 洩氣管 286 氣壓計 30 馬達 302 汲取部 304 第二輸送管 311 縫線 32 第二固定元件 322 插針 501 第三穿孔 601 第四穿孔 70 固定元件 80 保護層 24201242583 VI. Description of the invention: [Technical field to which the invention pertains] Especially suitable for blood. The present invention relates to a tissue engineering device for tissue engineering device related research. [Prior Art] The so-called tissue engineering, that is, the use of biologically active substances, through the method of in vitro culture or construction, to achieve the extravagance - > set times again 1 ε · benefits g and tissue technology. Tissue engineering has developed more than 20 cars.. For the rest of the year, it has been possible to reconstruct bones, skin, tendons, corneas and other tissues and organs. During tissue or organ repair or reconstruction, new tissue growth and appropriate blood vessels are needed to transport nutrients and remove waste. At present, it has been pointed out that various angiogenic factors have the functions of helping angiogenesis, tissue repair and regeneration. # In addition, due to congenital malformations, trauma, infections caused by tumor resection: head defects, is also one of the problems faced by tissue engineering. Therefore, current tissue engineering also has research on the repair of bone defects in different vascular compositions in angiogenesis, and in combination with different stem cells and terminal cells in combination with osteopathy. In order to establish a tissue engineering model for the above research, it has been developed to use the stone cervix to coat the arteries and veins as an engineering device. As shown! As shown, the tissue engineering device comprises a (four) ^ Λ L g 1 1 _ which is formed by crimping the _ (four) piece and suturing the stone eve by the suture (1). When the tissue engineering device is installed in a 201242583 When the subject to be observed is offered, the main body of the object to be observed is the hollow hole 112 of the miscellaneous tube 11. If the influence of different analytes on angiogenesis is to be studied, it can be administered in the hollow hole 112. However, the two sides of the 矽-, -, - 11 are not sealed, so when the object to be tested is placed in the hollow hole 112, the object to be tested may diffuse to the outside of the stone tube η Therefore, it is affected by the progress of the research. Accordingly, there is a need to develop a tissue engineering device, which can be used to study the effects of various analytes on angiogenesis. SUMMARY OF THE INVENTION The main object of the present invention is to provide a tissue The observation device is configured to seal the space of the vessel to be observed in this space. To achieve the above object, the tissue observation device of the present invention comprises: one: a half cylinder; a second half cylinder 'system and - half cylinders are combined with each other The first cylinder is provided with a first opening and the second end of the hollow cylinder has a first opening, and a first cover is assembled. The first opening of the first end of the hollow cylinder and the first opening of the bank _ τ 匕 二 , , , , , , , , 第 第 第 第 第 第 第 第 第 第 第 第 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一 第一1 〖Is here, use, this you use, 芏 less one," so it has been represented that there are two perforations. Therefore, in order to obtain a larger norm, it is recommended not to use the following sentence: and - the second cover body, which is disposed on the first end of the hollow cylinder and corresponding to the second opening of the hollow cylinder, through the second cover To open or close the second opening, the flute. ^ open and the second cover has at least one second through, and the Zhongtian tissue engineering device is set on the main body to be observed, waiting for 201242583 to observe the main body of the blood vessel through the sequence a first perforation of the first cover, a hollow cylinder, and a second perforation of the second cover. 3 The tissue engineering device of the present invention provides a confined space through which blood vessels can pass. Therefore, when the tissue engineering device of the present invention is installed on a subject to be observed (for example, a mouse), different test objects such as periosteum and oxygen can be placed in the empty cylinder of the tissue engineering device according to the purpose of observation. , growth factors, etc., different organisms assembled into polymers to study the effects of various analytes on angiogenesis. In the tissue engineering device of the present invention, the first perforation of the first cover and the second perforation of the second cover are respectively provided with a balloon, and the balloon is collapsed or collapsed to adjust the first-f The hole area of the hole and the second perforation. By providing a balloon, the remaining area of the first perforation and the second perforation through which the blood vessel passes can be filled to improve the sealing of the tissue device. When the sealing of tissue engineering equipment is improved, it can be applied to local vascular tissue engineering research: venous congesti〇n, insufficiency of arterial insufficiency. In the tissue engineering device of the present invention, the area of the holes for adjusting the first perforation and the second perforation can be transmitted in the following two ways. One of the methods can be: provided on the first cover body and the second cover body - an inflation valve m air valve 'inflating valve system is connected to the balloon to expand the balloon, and the money valve is connected to the balloon to collapse the balloon; Alternatively, the tissue engineering device may further include an inflator connected to the balloon, the inflator being inserted into the balloon of the 4 body to inflate the balloon. In addition, in the tissue engineering device of the present invention, the first half cylinder has a first joint surface. The second half cylinder has a second joint surface, and the first joint surface and the second joint surface correspond to each other. Here, the manner in which the first joint and the second joint are combined is not particularly limited 'can be combined using methods known in the art, for example: the first joint of the first half cylinder and the second joint of the second half cylinder The surface may have an L-shaped structure, and the L-shaped structure of the first half cylinder and the second half cylinder are mutually fitted; the first half-way, the first joint surface is provided with a plurality of jacks, and the second half-tube is second. The connecting surface is provided with a plurality of pins, the pin system and the socket are corresponding to each other, and when the first half cylinder and the second half cylinder are set as a hollow cylinder, the pin is inserted into the socket; the first half The first junction of the cylinder and the second junction of the second cylinder are respectively provided with a magnet 'and the first half cylinder and the third half cylinder are coupled to each other through the magnet; or the outer wall of the first cylinder is provided with a flange The outer wall of the second half cylinder is provided with a hook member, and the first half cylinder and the second half cylinder are assembled into a hollow cylinder body by hooking the hook member and the flange. Further, the two second junctions of the first half cylinder and the second half: the two second junctions ' may be combined in the same or phasewise manner. The first surface may respectively be provided with/seal the first joint surface and/or the second joint layer of the second half cylinder to improve the sealing property of the tissue engineering device, the second cover and the second cover body and the hollow cylinder body The adhesion is as follows: the woven engineering device may further comprise one piece, and the first end and the second end of the hollow cylinder are respectively provided with ice sand 厶 々堃Α 々堃Α 卓- the combination of the hole, the first knot and the second part of the hole are set on the first half of the tube, .. t t the first part of the combined hole is a square, the same one, the same as above, and when the first cover and The first body and the second body are fixed to the first and second covers when the body is in the middle of the 201242583 body of the tissue engineering device of the present invention. The group is arranged on the hollow cylinder. In addition, in order to prevent the first cover body and the second cover body from falling from the hollow cylinder body, the first end and the second end of the hollow cylinder body are respectively provided with a close groove, the first cover body and the second cover body The cover system is respectively provided with a close-contact portion, and when the first cover body and the second cover body are assembled in the hollow cylinder body, the close-up portion is stuck in the close-fitting groove. Further, in the tissue engineering device of the present invention, the first cover body and the second cover system are respectively provided with a ball bearing. By the arrangement of the ball bearing, the relative positions of the first cover and the second cover and the blood vessel can be fixed to prevent the blood vessel from being twisted due to the movement of the subject to be observed. Furthermore, in the tissue engineering device of the present invention, the first end and the second end of the hollow cylinder may be respectively provided with a sloped surface, wherein a protective layer is disposed on the inclined surface, and the material of the protective layer may be Shishijiao. Through this bevel, the angle of the edge of the tube wall can be alleviated, and the blood vessel is protected from damage due to the angular shape of the hollow tube wall. In another aspect, the tissue engineering device of the present invention may further include a circulation device including: a motor including an output portion and a scooping portion; and a first fixing member disposed on the main body to be observed a second fixing member is disposed on the main body to be observed; a first conveying pipe, the two ends of which are respectively connected with the output portion and the first fixing member; and a second conveying pipe, the two ends of which are respectively The tube body of the hollow cylinder has a fluid input port and a fluid output port, and the hollow cylinder further includes a fluid input tube and a fluid output tube, 201242583 Body: In: The two ends of the fluid inlet and the fluid connection to the first solid pipe are respectively connected to the second delivery pipe that flows to the first fixed reading. The fluid and the extraction portion draw the fluid so that the flow system sequentially =-: the delivery tube, the fluid input tube, the t-cavity tube, the fluid output tube, and the second delivery tube. Thereby, the purpose of (iv) liquid or gas exchange can be achieved. In this case, the tissue engineering device of the invention may further comprise a first inner half I and a first inner half and a first inner half cylinder and a second inner half cylinder joint 5 and assembled into a hollow inner cylinder. And the hollow inner cylinder is located in the hollow cylinder. A plurality of holes may be formed in the wall of the medium-working body and the hollow inner cylinder, and a space formed by the hollow cylinder and the hollow inner cylinder is provided with a permeable membrane. Preferably, the permeable membrane is a membrane having molecular weight selectivity. The molecular species delivered into the hollow inner cylinder can be adjusted through the arrangement of the permeable membrane. Furthermore, the tissue engineering device of the present invention may further comprise a plurality of permeable membranes that are fixed in the hollow cylinder; or may further comprise a plurality of separators, and a plurality of (four) membranes, the separators and (4) radially fixed to the membrane The inner space of the hollow cylinder can be separated by the arrangement of the π-permeable membrane and/or the partition in the hollow cylinder. If there are different internal pq 32, a j ^. The different materials to be tested are placed in the second chamber, and the type or size of the molecules transported in the hollow cylinder can be controlled by the pores and the selectivity of the membrane and the separator. In another aspect, the tissue engineering device of the present invention may further comprise a plurality of fixed elements ίτ "on the hollow body, the hollow tube system is fixed to the body to be observed through the fixing member. Thereby, the tissue engineering device can be fixed The relative position of the subject to be observed. 201242583 [Embodiment] The following describes the embodiments of the present invention by way of specific embodiments, and those skilled in the art can readily understand other advantages of the present invention from the disclosure of the present specification. The invention may be embodied or applied by other different embodiments, and various details in the specification are also based on various aspects and applications, and various modifications and changes can be made without departing from the spirit of the invention. Embodiment 1 FIG. 2 is a schematic view of the tissue engineering device of the present embodiment installed on a main body to be observed. The tissue engineering device of the present embodiment includes: a first half cylinder 21 and a second half cylinder 22 a first cover body 23 and a second cover body 24. When the tissue engineering device of the embodiment is assembled on the body to be observed! The blood vessel 101 of the body 10 passes through the perforations provided in the first cover body 23 and the second cover body 24 (not shown). Next, the structure of the tissue engineering apparatus of the present embodiment will be described in detail. As shown, the tissue engineering device of the present embodiment includes: a first half cylinder 2 1 ; a second half cylinder 22 ′ and the first half cylinder 2 1 are combined with each other to form a hollow cylinder 25 ' hollow cylinder The first end of the body 25 has a first opening 251, and the second end of the hollow cylinder 25 has a second opening 252. A first cover 23 is disposed at the first end of the hollow cylinder 25. The first opening 25 丨 ' corresponding to the hollow cylinder 25 passes through the opening and closing of the first cover 23 to open or close the first opening 251 ′ and the first cover 23 has at least one first through hole 23 1 ; A second cover body 24' is disposed on the second end of the hollow cylinder 25 and corresponding to the second opening 252 of the hollow cylinder 25, through the opening of the second cover body 24 201242583 = opening or closing the second opening σ 252, and the second cover body 24 has at least - the first = hole 241. Wherein, when the tissue engineering device is set to - the body to be observed β and % winter read Figure 2), the vasospasm (1) of the subject to be observed is sequentially passed through the first-perforation 23丨 of the corpus callosum 23, the hollow cylinder 25, and the second cover 24 241. In the present embodiment, the first cover 23 has two first through holes 231, and the cover 24 has two second through holes 241, and the two first through holes 231 and the second one. The iliac crest is used to pass the arteries and veins respectively. Therefore, when the first cover body 23 and the second cover body 24 are assembled on the hollow cylinder 25, a closed space can be formed for tissue engineering research and blood observation. In addition, in order to fix the first cover 23 and the second cover 24 to the hollow tubular leg 25_^, the flange 232 of the first cover 23 is provided with a close portion 233, and the second cover is provided. A flange 242 of the body 24 is also provided with a close-fitting portion 243, and a closed groove 253 is respectively disposed at both ends of the inner body of the ten-hole body 25, and the close-fitting grooves 253 at the two ends respectively correspond to the first portion The adhesion portion 233 of the lid body 23 and the second lid body 24 are in close contact portion 243. Therefore, the first lid body 23 and the second lid body 24 can be assembled and fixed to both ends of the hollow cylinder 25 by sandwiching the adhesion portions 233, 243 in the adhesion groove 253. Next, the manner of joining the first half cylinder 2''' to the second half cylinder 22 of the present embodiment will be described. In the present embodiment, the two junctions of the first half cylinder 21 and the second half cylinder 22 are joined in different ways. As shown in FIG. 3A, the first half cylinder 2 has a first joint surface 2丨1, and the second half cylinder 22 has a second joint surface 22][, and the first joint surface 2 1 1 is connected to the second joint. The faces 22 1 correspond to each other. Wherein, one of the first junction 2丨1 of the first half cylinder 2 1 and the second junction 22 1 of the second half cylinder 22 respectively have a corresponding structure [type structure, so when the first When the half cylinder 2 丨 and the second half cylinder 22 are assembled into a hollow cylinder, the first half cylinder 2 丨 and the second half cylinder are "fitted by the L-shaped structure. In addition, in the embodiment A, the first The combination of the other first joint 2! i of the half cylinder 2 1 and the other second joint 221 of the second half cylinder 22 can be combined by a hinge. As shown in Fig. 3C, the first half cylinder 21 The outer wall and the outer wall of the second half cylinder 22 are provided with a hinge 29. Therefore, in the present embodiment, the joint faces of the first half cylinder 2 and the second cylinder 22 are joined by an L-shaped structure and a hinge. The joint faces of the first half cylinder 21 and the first half cylinder 22 can also be joined in the same manner to form a hollow cylinder. Embodiment 2 FIG. 4 is a schematic view of a tissue engineering apparatus according to the present embodiment. The tissue engineering device is similar to the tissue engineering device of Embodiment 1, except that the first half cylinder and the second half cylinder are combined, and the first cover and the second cover are combined Different from Embodiment 1. As shown in FIG. 4, the first half cylinder 21 has a first joint 2丨1, the second half 22 has a second joint 221, and the first joint 211 is second. The connecting surfaces 221 correspond to each other. In the embodiment, the first connecting surface 21 of the first half cylinder 21 and the second connecting surface 22 1 of the first half cylinder 22 respectively have an l-type structure. When the first half cylinder 21 and the second half cylinder 22 are assembled into a hollow cylinder, the first half cylinder 21 and the second half cylinder 22 are fitted to each other by an L-shaped structure. 201242583 On the other hand, the first half The other first joint 2 1 1 of the cylinder 2 1 is provided with a plurality of jacks 2 111 'the other second joint 22 of the second half cylinder 22 is provided with a plurality of pins 22 1 1 'the second joint The pin 22 1 on the 22 1 corresponds to the intervening 2111 of the first joint 2 1 1 , and when the first half cylinder 21 and the second half cylinder 22 are assembled into a hollow cylinder, the pins 2211 are inserted. The sheath is formed in the insertion hole 2111 as shown in FIG. 4 and FIG. 5(A). Further, on the first connection surface 211 of the first half cylinder 21, a sealing layer 26 may be further disposed through the sealing layer 26. The first junction 2丨丨 and the second junction 2 2 1 can be made more In this embodiment, the first half cylinder 2 1 and the second half cylinder 22 are joined in two different ways (L-shaped structure, and pins and jacks) to form a hollow cylinder, as shown in the figure. 4 and Fig. 5(A). However, the joint faces of the first half cylinder 2丨 and the second half cylinder 22 can also be joined in the same manner to form a hollow cylinder. On the other hand, in the organization of the embodiment The engineering device, the first cover U and the first cover 24 are respectively provided with a ball bearing 244 (here, the ball bearing of the first cover 23 is not shown), wherein the ball bearing 244 comprises: an outer ring 244! One inside 2442, and a plurality of balls 2443, the ball 2443 is disposed between the outer % 244 1 and the inner ring 2442' and the outer ring μ of the ball bearing 244 is used as a close portion to be stuck in the close groove (5) Medium. When the tissue engineering device of the embodiment is assembled on the main body to be observed, the ball bearing (10) can fix the relative positions of the first through hole 231 and the first through hole 241 and the blood vessel to prevent the hollow cylinder from moving due to the struggle of the main body to be observed. And distort the blood official of the subject to be observed. Further, the first cover 23 has a first through hole 23, and the second cover 201242583 24 has a second through hole 24. Therefore, the tissue device of the present embodiment can pass only one blood vessel. In addition to the use of an L-shaped structure, and a pin and a jack, the first half cylinder 21 and the second half cylinder 22 may be combined in other ways as described below. As shown in Fig. 5(B), the first joint surface 2 of the first half cylinder 2 and the second joint surface 221 of the second cylinder 22 have a circular arc structure corresponding to each other. As shown in FIG. 5(C), the first junction 211 of the first half cylinder 21 and the second junction 22 1 of the second cylinder 22 are respectively provided with a magnet 21 1 2, 22 12 and the first half cylinder 2 1 and the second half cylinder 22 are transmitted through the magnets 2 112, and the magnetic force of the 22 1 2 is mutually gentle. As shown in FIG. 5(D), the outer wall of the first half cylinder 21 is provided with a flange 2n. The outer wall of the second half cylinder 22 is provided with a hook member 223, and is fastened by the hook member 223 and the flange 213. The first half cylinder 2 丨 and the second half cylinder 22 are grouped into the hollow cylinder (not shown). Embodiment 3 Fig. 6 is a schematic view of a tissue engineering apparatus of the present embodiment. The tissue engineering apparatus of this embodiment is similar to the tissue engineering apparatus of the first embodiment except that the first and second half cylinders are combined, and the first and second cover structures are different from the first embodiment. Here, only the first cover portion of the tissue engineering device will be described in detail. As shown in the figure, the tissue engineering apparatus of the present embodiment further includes a lock member 27 which is attached to the first cover body 23. Wherein the first hollow end of the hollow tubular body is provided with a first joint 254 ′, the first portion 212 of the first joint hole 254 is disposed on the first half cylinder 21, and the second joint hole 254 is second. The portion 222 is provided on the second half cylinder 22. Therefore, when the first cover 23 is assembled to the hollow cylinder 25 14 201242583 : 'the lock member 27 is inserted into the first coupling hole 254 , the lock member 27 can be fixed in the first coupling hole 254 by rotating the lock member 27 The first cover 23 is fixedly mounted on the hollow cylinder 25 . By engaging the lock member 27 in the 'binding hole 2M', the separation of the first-half cylinder 21 and the second half cylinder 22 can be prevented. In the tenth embodiment, the locking portion 27 of the lock member 27 is corresponding to the shape of the first coupling hole 254, and is of a type. In addition, in the tissue engineering device of the embodiment, the first cover h has a first through hole 231 'the second cover (not shown) also has a second through hole corresponding to the first cover 23 ( The structure of the present invention is such that only one blood vessel can pass. In the tissue engineering device of the embodiment, since the first cover body and the second ancestor group are the same in the hollow cylinder, only the first cover body is disposed at the first end of the hollow cylinder. The situation. Embodiment 4 As shown in Fig. 7, this is a schematic view of the tissue engineering apparatus of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the third embodiment. The locking portion 271 of the lock member 27 is S-shaped. Embodiment 5 Fig. 8(A) is a partial schematic view showing a tissue engineering apparatus provided with a space adjusting device of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the second embodiment except that the tissue engineering device of the present embodiment is further provided with a space adjusting device. As shown in Fig. 8 (Α), in the tissue engineering apparatus of the present embodiment, a balloon 28 is provided in the first through hole 23 of the first cover 23 . In addition, the first cover body 2 3 is further provided with an inflation valve 2 8 1 and an airless valve 2 8 2 ', wherein the inflation valve 2 8 1 201242583 is connected to the balloon 28 through an inflation tube 283 to pass the gas into the air. The balloon 28 is inflated in the balloon 28; and the deflation valve 282 is coupled to the balloon 28 through a deflation tube 284. The excess gas is expelled from the balloon 28 by the deflation tube 284. Therefore, in the tissue engineering apparatus of the present embodiment, the hole area of the first perforation 23 1 can be adjusted by the collapse or expansion of the balloon 28. Thereby, when the tissue engineering device of the embodiment is installed on a main body to be observed, since the balloon 28 can adjust the hole area of the first perforation 213, the vascular passage 1 0 1 can be more encrypted. The first perforation 23 1 'is improved the tightness of the tissue engineering device. In the tissue engineering device of the present embodiment, since the first cover body and the second cover body are provided with the same space adjustment device, only the space adjustment device disposed on the first cover body will be described herein. Fig. 8(B) is a partial schematic view showing the tissue engineering device provided with the space adjusting device of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the fifth embodiment except that the space adjusting device of the present embodiment includes: a balloon, and a charger device. As shown in Fig. 8(B), a balloon 28 is provided in the first through hole 231 of the first cover 23 in the tissue engineering apparatus of the present embodiment. In addition, the tissue engineering device of the present embodiment further includes an inflator 285 connected to the balloon 28 through an inflation tube 283, and the inflator 285 is configured to input a gas into the balloon to expand the balloon 28. Further, the space adjustment device is further Including a barometer 286 coupled to the inflator 285 to adjust the flow rate, volume, or pressure of the gas input into the balloon 28. 201242583 In the tissue work apparatus of the present Palladium, due to the first cover A perforation and a second perforation of the second body are provided with the same space adjustment device, so only the space adjustment device assembled on the first cover body will be described herein. Further, it is disposed in the first perforation and the second perforation. The balloon can be inflated using the same filling device. Embodiment 7 Fig. 9 is a schematic view of the tissue engineering device incorporating the circulation device of the embodiment. The tissue engineering device of the embodiment is the tissue engineering device of the embodiment 2. Similarly, the tissue engineering device of the embodiment further includes a circulation device. As shown in FIG. 9, the tissue engineering device of the embodiment further includes a circulation device, and the package thereof Included: a motor 30 comprising a wheeled portion 3 (H, and a picking portion 302; - a first jaw element η, which is assembled on the skin 103 of the body to be observed; a second fixing member 32, The group is disposed on the skin 103 of the body to be observed (7): a first conveying pipe 3〇3, the two ends of which are respectively connected with the output part training first fixing element 3!; and the second conveying pipe 3() 4, The two ends are respectively connected to the capturing portion 302 and the second fixing member 32. Here, the first fixing member 31 can be fixed to the skin of the body to be observed through the suture 3丨1 or the pin 3〗2. 3 surface and tissue 丨〇 2; and the second fixing element u can also be fixed to the body 10 to be observed in the same manner as the first fixing element 3 缝 (the suture 32 丨 or the pin 322). The wall of the pipe has a fluid wheel inlet 255, a fluid output σ 256', and the hollow cylinder 25 further includes a fluid input pipe W and a fluid output pipe 258. The fluid input pipe 257 is respectively connected to the fluid 201242583 The input port 255 is connected to the first wheel feed pipe connected to the first fixing component 3i, and the two ends of the fluid output pipe 258 are respectively The body output port 258 is connected to the first transfer pipe 304 connected to the first solid-state element 32. Therefore, in the present embodiment, the output unit 301 of the motor 30 outputs the fluid, and the pumping unit 3〇2 draws the fluid. The flow system sequentially flows through the first transfer pipe 3〇3, the fluid input pipe 257, the hollow body 25, the fluid output pipe 258, and the second transfer pipe 304. By this, the purpose of gas or liquid exchange can be achieved. With this circulation device, the tissue engineering device of the present embodiment can be used in various studies affecting angiogenesis factors, such as growth factor regulation oxygen/carbon dioxide exchange, hyper/hyp〇baHc oxygen therapy, and the like. Further, if a heater and a negative feedback temperature sensor are added to the tissue processing apparatus of the present embodiment, the temperature can be adjusted or used to investigate the influence of temperature on the blood vessel. Embodiment 8 Fig. 0 is a schematic view of a tissue engineering apparatus of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the second embodiment. In addition to the tissue engineering device of the present embodiment, a hollow inner cylinder is further provided. As shown in FIG. 10, the tissue engineering device of the present embodiment further includes an inner half cylinder 215 and a second inner half cylinder 225. The first inner half cylinder and the second inner half 225 series are combined with each other. Set to - hollow inner cylinder. In the present embodiment, the inner half-half 215 corresponds to the first half cylinder 21 and is disposed inside the first half cylinder, and the second inner half cylinder 225 corresponds to the second half cylinder 22 and is disposed in the second half cylinder. 22 inside. Therefore, when the first half cylinder 2| and the second half cylinder 22 are assembled into a hollow 201242583 cylinder, and the first inner half cylinder 2丨5 and the second inner half cylinder 225 are assembled into a hollow inner cylinder, the hollow inner cylinder It is located in the hollow cylinder. Further, in the tissue engineering apparatus of the present embodiment, the hollow cylinders (ie, the first half cylinder 21 and the second cylinder 22) and the hollow inner cylinder (ie, the first inner cylinder 215 and the first inner cylinder 225) The pipe wall system is provided with a plurality of holes 2 [4, 224, Μ. 225 1, and a space formed by the hollow cylinder and the hollow inner cylinder is provided with a seepage film 40. Further, the permeable membrane 40 disposed between the hollow cylinder and the hollow inner cylinder is replaceable. Embodiment 9 Fig. 1 is a schematic view showing a tissue engineering apparatus of this embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the first embodiment except that the tissue engineering device of the present embodiment further includes a plurality of permeable membranes 5 〇 which are radially fixed in the hollow cylinder 25. At the same time, the permeable membrane 5〇 also has a third perforation 5〇1 to pass the blood vessel of the subject to be observed. More specifically, in the tissue engineering apparatus of the present embodiment, the inner wall of the hollow cylinder 25 is provided with a fixing groove 259' and the permeable membrane 5' is radially fixed to the fixing recess 259. When the first half cylinder 21 and the second half cylinder 22 are assembled into a hollow cylinder, the permeable membrane 50 can divide the inner space of the hollow cylinder into a plurality of small spaces. Since the / permeable membrane 50 is a molecularly-selective film that allows a molecule having a specific size to pass through, a selective release substance can be obtained by placing a different substance in a small space into which the hollow cylinder is divided. The purpose. Embodiment 10 Figure 1 2 is a schematic view of the tissue engineering apparatus of the present embodiment. The tissue working device of the present embodiment is similar to the tissue engineering device of the embodiment 9, except that the tissue engineering device of the embodiment of the 201242583 embodiment further includes a plurality of partitions 60 which are radially fixed to the hollow cylinder in the same manner as the permeable membrane 50. Within body 25. Further, the partition 6 has a fourth perforation 601 ' to pass the blood vessel of the subject to be observed. Further, the spacer 60 further has a plurality of through holes 6〇2 penetrating through the spacers 6〇. As shown in FIG. 12, the tissue engineering device of the present embodiment is provided with a permeable membrane 5〇 and a partition 60 from the axial direction of the first cover 23 or the second cover 24, and the hollow cylinder can be disposed. The internal space is divided into several small spaces. Here, the car is placed in the space formed by the permeable membrane 5 〇 and the partition 6 放置, and the substance to be studied can be transported to the two partitions by the fourth perforation 6 〇 1 of the partition 6 〇 In the space formed by the plate 60, the use of the molecular weight selective permeable membrane 50 prevents the substance to be studied from being transferred into the space formed by the permeable membrane 5 and the first cover 23 or the second cover 24. In addition, in order to make the space separated by the permeable membrane 50 and the partition 6 具有 sealed, a space adjusting device can be selectively disposed in the third through hole 5〇1 and the fourth through hole 6〇1, as shown in FIG. 8(A). ) or as shown in Figure 8 (B). Embodiment 11 Fig. 1 is a schematic view showing the first end of a hollow cylinder of the tissue engineering apparatus of the present embodiment. The tissue engineering device of the embodiment is similar to the tissue engineering device of the embodiment 9, except that the first end and the second end (not shown) of the hollow cylinder of the tissue engineering device of the embodiment are respectively provided with a slope 2511. A protective layer 70 is disposed on the slope 25丨1, and the material of the protective layer 70 is silicone. Since the first end and the second end of the hollow cylinder of the tissue engineering apparatus of the embodiment are provided with a slope and a protective layer, the blood vessel can be protected from being damaged by the edge of the hollow cylinder. 201242583 Embodiment 12 FIG. 14 is a schematic view of the tissue engineering apparatus of the present embodiment. In this embodiment, the weaving device is similar to the tissue engineering device of the second embodiment, except that the tissue maintenance device of the present invention includes a plurality of solid components 70, which are disposed on the second cylinder 25, and The hollow cylinder 25 is passed through the fixing member 7 to be fixed to the body of the 'τ' (not shown). In more detail, one of the fixing elements is terminated. Further, on the outer side of the hollow body 25, and the other end of the fixing member 7 is provided with a boring hole 70丨', and the fixing τ 件 70 is fixed by a suture (not shown) passing through the fixing hole 丨. On the subject to be observed (not shown). Embodiment 13 Fig. 15 is a schematic view showing a tissue engineering apparatus of the present embodiment. The tissue engineering apparatus of this embodiment is similar to the tissue processing apparatus of the first embodiment, except that the hollow cylinder system of the tissue engineering apparatus of the present embodiment is square and a partition 60 is provided at the bottom of the hollow cylinder. Therefore, when the tissue engineering device of the embodiment is installed on the main body to be observed, the space above the partition 60 is provided with a space under the partition (4) for placing the blood vessel of the main body to be observed, and the substance to be studied is transmitted through The through hole 6〇2 of the partition plate 60 opens into the space in which the blood vessel is placed (i.e., the partition space). The above-described embodiments are merely examples for the convenience of the description, and the scope of the claims of the present invention is intended to be based on the scope of the claims and not limited to the above embodiments. [Simple description of the drawing] 201242583 Figure 1 is a conventional tissue engineering device. Fig. 2 is a schematic view showing the arrangement of the tissue engineering device of the embodiment of the present invention on the main body to be observed. Fig. 3A is an exploded perspective view showing the first cover body and the second cover body of the tissue engineering device according to the embodiment of the present invention. Fig. 3B is a schematic view showing one side of the first half cylinder and the second half cylinder of the tissue engineering apparatus of the first embodiment of the present invention. Fig. 3C is a schematic view showing the other side of the first half cylinder and the second half cylinder of the tissue engineering apparatus of the first embodiment of the present invention. Fig. 4 is a diagram showing the attenuation of the tissue engineering apparatus of the second embodiment of the present invention. Fig. 5 is a schematic view showing the combination of the first half cylinder and the second half cylinder of the tissue engineering apparatus of the present invention. Fig. 6 is a schematic view showing the first cover body of the tissue engineering apparatus of the third embodiment of the present invention assembled in a hollow cylinder. Fig. 7 is a partial schematic view showing the first cover body of the tissue maintenance device of the fourth embodiment of the present invention disposed in the hollow cylinder. . Fig. 8 is a partial schematic view showing a tissue engineering device provided with a space adjusting device according to a fifth embodiment of the present invention. Fig. 9 is a partial schematic view showing a tissue engineering device provided with a space adjusting device according to a sixth embodiment of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 10 is a schematic view of a tissue engineering device according to a seventh embodiment of the present invention. Fig. 11 is a schematic view showing a tissue engineering device according to a ninth embodiment of the present invention. 201242583 Fig. 1 2 is a schematic view of a tissue engineering device according to Embodiment 10 of the present invention. Fig. 3 is a schematic view showing a first end of a hollow cylinder of a tissue engineering device according to Embodiment 11 of the present invention. Figure 1 is a schematic view of a tissue engineering device of Embodiment 13. Figure 15 is a schematic view of a tissue engineering device according to Embodiment 13 of the present invention. * [Main component symbol description] 10 Subject to be observed 101 Blood vessel 102 Tissue 103 Skin 11 Silicone tube 111 Suture 112 Hollow hole 21 first half cylinder 21 1 first joint surface 2111 socket 2112, 2212 magnet 212 first portion 213 flange 214, 224, 2151, 2251 hole 215 first inner half cylinder 22 second half Cartridge 221 second joint 2211 pin 222 second portion 223 hook member 225 second inner half cylinder 23 first cover body 23 1 first through hole 232 flange 233 contact portion 24 second cover body 241 second through hole 242 Flange 243 Closer 244 Ball bearing 2441 Outer ring 2442 Inner ring 2443 Ball 25 Hollow cylinder First opening Second opening First joint hole Fluid output port Fluid output tube Sealing layer Carding part Inflatable chamber Inflator tube Inflator Hinge Output section First delivery tube First fixing element Pin suture osmosis membrane plate penetration hole holder 匕2511 Bevel 253 Closer groove 255 Fluid input port 257 Fluid input tube 259 Fixing groove 27 Locking member 28 Balloon 282 Valve 284 bleed tube 286 barometer 30 motor 302 raking portion 304 second delivery tube 311 suture 32 second fixation element 322 pin 501 third perforation 601 fourth perforation 70 fixation element 80 protective layer 24