TWI446899B - Tissue engineering device - Google Patents

Description

Tissue engineering device

The present invention relates to a tissue engineering device, and more particularly to a tissue engineering device suitable for vascular related research.

The so-called tissue engineering is a technique for repairing or regenerating organs and tissues by using biologically active substances through in vitro culture or construction. Tissue engineering has been developed for more than 20 years, and it is now possible to reconstruct bones, skin, tendons, corneas and other tissues and organs.

During tissue or organ repair or reconstruction, new tissue growth and development requires appropriate blood vessels to deliver nutrients and remove waste. At present, it has been pointed out that a variety of vascular growth factors have functions of helping angiogenesis, tissue repair, and regeneration.

In addition, bone defects caused by congenital malformations, trauma, infection, and tumor resection are also one of the problems faced by tissue engineering. Therefore, current tissue engineering also has a role in the role of different vascular composition in angiogenesis, as well as the combination of different stem cells and terminal cells combined with bone-inducing ability of the periosteum to help repair bone defects.

In order to establish a tissue engineering model for the above research, it has been developed to use enamel to coat arteries and veins as a tissue engineering device for promoting vascular proliferation. As shown in Fig. 1, the tissue engineering device comprises a silicone tube 11 formed by crimping a film and sewing the film to the opposite sides through a suture 111. When the tissue engineering device is mounted on a body 10 to be observed, the blood vessel 101 of the body 10 to be observed passes through the hollow hole 112 of the rubber tube 11. If the influence of different analytes on angiogenesis is to be studied, the analyte can be administered in the hollow hole 112.

However, the ends of the rubber hose 11 are not sealed, so when the object to be tested is placed in the hollow hole 112, the object to be tested may diffuse outside the silicone tube 11, which affects the progress of the research. Accordingly, there is a need to develop a tissue engineering device that can be effectively used to study the effects of various analytes on angiogenesis.

SUMMARY OF THE INVENTION A primary object of the present invention is to provide a tissue viewing device that provides a space for sealing a region of a blood vessel to be viewed in this space.

In order to achieve the above object, the tissue observation device of the present invention comprises: a first half cylinder; a second half cylinder combined with the first half cylinder to form a hollow cylinder, the first end of the hollow cylinder The first end of the hollow cylinder has a second opening; a first cover is disposed on the first end of the hollow cylinder and corresponds to the first opening of the hollow cylinder. The first opening is opened or closed by the opening and closing of the first cover, and the first cover has at least one first through hole (herein, "at least one" is used, so it has been represented that there may be two perforations. Therefore, in order to obtain a larger range, it is recommended not to add a third perforation] ; and a second cover body is disposed on the second end of the hollow cylinder and corresponding to the second opening of the hollow cylinder, through the second Opening and closing the cover to open or close the second opening, and the second cover has at least one second through hole; wherein, when the tissue engineering device is assembled on a body to be observed, the blood vessel of the main body to be observed is sequentially worn The first perforation of the first cover, the hollow cylinder, and the second perforation of the second cover.

The tissue engineering device of the present invention provides a confined space through which blood vessels can pass. Therefore, when the tissue engineering device of the present invention is installed on a main body to be observed (for example, a mouse), different test objects such as periosteum, oxygen, and growth may be placed in the hollow cylinder of the tissue engineering device according to the purpose of observation. Factor, etc., a collection of different organisms to study the effects of various analytes on angiogenesis.

In the tissue engineering device of the present invention, a balloon is respectively disposed in the first perforation of the first cover and the second perforation of the second cover, and the first perforation is adjusted by the balloon being collapsed or expanded. The hole area of the second perforation. By providing a balloon, the remaining area of the first perforation and the second perforation through which the blood vessel passes can be filled to improve the sealing of the tissue engineering device. When the sealing performance of the tissue engineering device is improved, it can be applied to local vascular tissue engineering research, venous congestion, arterial insufficiency and the like.

In the tissue engineering device of the present invention, the area of the holes for adjusting the first perforation and the second perforation can be transmitted in the following two ways. One of the methods may be: an inflation valve and a deflation valve may be further disposed on the first cover body and the second cover body, the inflation valve system is connected to the balloon to expand the balloon, and the deflation valve is connected to the balloon to collapse the balloon. Alternatively, the tissue engineering device may further include an inflator connected to the balloon, the inflator being inserted into a balloon of gas to inflate the balloon.

Further, in the tissue engineering device of the present invention, the first half cylinder has a first joint surface, the second half cylinder has a second joint surface, and the first joint surface and the second joint surface correspond to each other. Here, the manner of combining the first joint and the second joint is not particularly limited, and may be combined in a manner known in the art, for example, the first joint of the first half cylinder and the second joint of the second half cylinder The face may have an L-shaped structure, and the L-shaped structure of the first half cylinder and the second half cylinder are mutually fitted; the first joint of the first half cylinder is provided with a plurality of jacks, and the second half of the cylinders The two junctions are provided with a plurality of pins, the pins are corresponding to the sockets, and when the first half cylinders and the second half cylinders are arranged as a hollow cylinder, the pins are inserted into the sockets; The first junction of the half cylinder and the second junction of the second cylinder are respectively provided with a magnet, and the first half cylinder and the second cylinder are coupled to each other through the magnet; or the outer wall of the first cylinder is provided with a convex The rim is provided with a hook member on the outer wall of the second half cylinder, and the first half cylinder and the second half cylinder are assembled into a hollow cylinder body by hooking the hook member and the flange. In addition, the two first joints of the first half cylinder and the two second joints of the second half cylinder may be combined in the same or phase.

On the other hand, in the tissue engineering device of the present invention, the first joint surface of the first half cylinder and/or the second joint surface of the second half cylinder may be respectively provided with a sealing layer to improve the sealing property of the tissue engineering device.

In order to strengthen the adhesion between the first cover body and the second cover body and the hollow cylinder body, the tissue engineering device of the present invention may further comprise a lock member, wherein the first end and the second end of the hollow cylinder body are respectively a first coupling hole is disposed, the first portion of the first coupling hole is disposed on the first half cylinder, and the second portion of the first coupling hole is disposed on the second half cylinder, and the first cover body and the second cover When the cover body is disposed on the hollow cylinder body, the lock member is inserted into the first joint hole to fix the first cover body and the second cover body on the hollow cylinder body.

In addition, in order to prevent the first cover body and the second cover body from falling from the hollow cylinder body, the first end and the second end of the hollow cylinder body are respectively provided with a close groove, the first cover body and the second cover body The cover system is respectively provided with a close-contact portion, and when the first cover body and the second cover body are assembled in the hollow cylinder body, the close-up portion is stuck in the close-fitting groove.

Further, in the tissue engineering device of the present invention, the first cover body and the second cover system are respectively provided with a ball bearing. By the arrangement of the ball bearing, the relative positions of the first cover and the second cover and the blood vessel can be fixed to prevent the blood vessel from being twisted due to the movement of the subject to be observed.

Furthermore, in the tissue engineering device of the present invention, the first end and the second end of the hollow cylinder may be respectively provided with a sloped surface, wherein the protective layer is provided on the inclined surface, and the material of the protective layer may be silicone. Through this bevel, the angle of the edge of the tube wall can be alleviated, and the blood vessel is protected from damage due to the angular shape of the hollow tube wall.

In another aspect, the tissue engineering device of the present invention may further include a circulation device including: a motor including an output portion and a scooping portion; and a first fixing member disposed on the main body to be observed a second fixing member is disposed on the main body to be observed; a first conveying pipe has two ends connected to the output portion and the first fixing member respectively; and a second conveying pipe, the two ends of which are respectively And the pumping portion is connected to the second fixing component; wherein the wall of the hollow cylinder has a fluid input port and a fluid output port, and the hollow cylinder further comprises a fluid input pipe and a fluid output pipe, the fluid The two ends of the input pipe are respectively connected with the fluid input port and the first conveying pipe connected to the first fixing component, and the two ends of the fluid output pipe are respectively connected with the fluid output port and the second conveying pipe connected to the second fixing component. The connection is performed, and the fluid is discharged through the output portion of the motor and the pumping portion draws the fluid, so that the flow system sequentially flows through the first conveying pipe, the fluid input pipe, the hollow cylinder, the fluid output pipe, and the second conveying pipe. Thereby, the purpose of liquid or gas exchange can be achieved.

In addition, the tissue engineering device of the present invention may further include a first inner half cylinder and a second inner half cylinder, and the first inner half cylinder and the second inner half cylinder are combined with each other to form a hollow inner cylinder, and The hollow inner cylinder is located in the hollow cylinder. Here, the wall of the hollow cylinder and the hollow inner cylinder may be provided with a plurality of holes, and a space formed by the hollow cylinder and the hollow inner cylinder is provided with a permeable membrane. Preferably, the permeable membrane is a membrane having molecular weight selectivity. Through the arrangement of the permeable membrane, the molecular species delivered into the hollow inner cylinder can be adjusted.

Furthermore, the tissue engineering device of the present invention may further comprise a plurality of permeable membranes radially fixed in the hollow cylinder; or may further comprise a plurality of separators and a plurality of permeable membranes, the separators and the permeable membrane being radially fixed to The hollow cylinder is inside. The inner space of the hollow cylinder can be separated by the arrangement of the permeable membrane and/or the partition. If different analytes are placed in different internal spaces, the type or size of the molecules transported in the hollow cylinder can be controlled due to the selectivity of the pores of the permeable membrane and/or the separator.

In another aspect, the tissue engineering device of the present invention may further comprise a plurality of fixing elements disposed on the hollow cylinder, and the hollow cylinder system is fixed to the body to be observed through the fixing member. Thereby, the relative position of the tissue engineering device on the body to be observed can be fixed.

The embodiments of the present invention are described by way of specific examples, and those skilled in the art can readily appreciate the other advantages and advantages of the present invention. The present invention may be embodied or applied in various other specific embodiments, and various modifications and changes can be made without departing from the spirit and scope of the invention.

Example 1

2 is a schematic view showing the tissue engineering device of the embodiment mounted on a main body to be observed. The tissue engineering device of the embodiment includes a first half cylinder 21, a second half cylinder 22, a first cover body 23, and a second cover body 24. When the tissue engineering device of the embodiment is assembled on a body 10 to be observed, the blood vessel 101 of the body 10 to be observed passes through the perforations provided on the first cover 23 and the second cover 24 (not shown in the figure) ).

Next, the structure of the tissue engineering apparatus of the present embodiment will be described in detail. As shown in FIG. 3A, the tissue engineering device of the present embodiment includes: a first half cylinder 21; a second half cylinder 22, which is combined with the first half cylinder 21 to form a hollow cylinder 25, which is hollow. The first end of the tubular body 25 has a first opening 251, and the second end of the hollow cylinder 25 has a second opening 252; a first cover 23 is disposed first in the hollow cylinder 25 a first opening 251 corresponding to the hollow cylinder 25, through the opening and closing of the first cover 23 to open or close the first opening 251, and the first cover 23 has at least one first through hole 231; The second cover body 24 is disposed on the second end of the hollow cylinder 25 and corresponding to the second opening 252 of the hollow cylinder 25, and is opened or closed by the opening and closing of the second cover body 24 to open or close the second opening 252. The second cover 24 has at least one second through hole 241. Wherein, when the tissue engineering device is disposed on a body 10 to be observed (please refer to FIG. 2 at the same time), the blood vessel 101 of the body 10 to be observed sequentially passes through the first through hole 231 of the first cover body 23 and the hollow cylinder. 25, and a second through hole 241 of the second cover body 24.

In this embodiment, the first cover 23 has two first through holes 231, the second cover 24 has two second through holes 241, and the two first through holes 231 and the second through holes 241 are respectively used. Pass the arteries and veins.

Therefore, when the first cover body 23 and the second cover body 24 are assembled on the hollow cylinder 25, a closed space can be formed for tissue engineering research and observation of blood vessels.

In addition, in order to fix the first cover 23 and the second cover 24 to the hollow cylinder 25, the flange 232 of the first cover 23 is provided with a close portion 233, and the second cover 24 is convex. The edge 242 is also provided with a close-fitting portion 243, and the inner ends of the hollow cylinder 25 are respectively provided with a close-fitting groove 253, and the close-fitting grooves 253 at the two ends respectively correspond to the denseness of the first cover 23 The joint portion 233 and the second lid body 24 are in close contact portion 243. Therefore, the first cover body 23 and the second cover body 24 can be assembled and fixed to both ends of the hollow cylindrical body 25 by inserting the adhesion portions 233, 243 in the adhesion grooves 253.

Next, the manner of joining the first half cylinder 21 and the second half cylinder 22 of the present embodiment will be described. In this embodiment, the two junctions of the first half cylinder 21 and the second half cylinder 22 are joined in different ways. As shown in FIG. 3B, the first half cylinder 21 has a first joint surface 211, the second half cylinder 22 has a second joint surface 221, and the first joint surface 211 and the second joint surface 221 correspond to each other. Wherein, one of the first joint surface 211 of the first half cylinder 21 and the second joint surface 221 of the second half cylinder 22 respectively have an L-shaped structure corresponding to each other, so when the first half cylinder 21 and the first When the two half cylinders 22 are arranged in a hollow cylinder, the first cylinders 21 and the second cylinders 22 are fitted to each other by an L-shaped structure.

In addition, in this embodiment, the combination of the other first joint surface 211 of the first half cylinder 21 and the other second joint surface 221 of the second half cylinder 22 may be combined by a hinge. As shown in FIG. 3C, the outer wall of the first half cylinder 21 and the outer wall of the second half cylinder 22 are provided with a hinge 29.

Therefore, in the present embodiment, the joint faces of the first half cylinder 21 and the second half cylinder 22 are joined by an L-shaped structure and a hinged manner, respectively. However, the joint faces of the first half cylinder 21 and the second half cylinder 22 can also be joined in the same manner to be assembled into a hollow cylinder.

Example 2

4 is a schematic view of the tissue engineering apparatus of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the first embodiment except that the first half cylinder and the second half cylinder are combined, and the first cover and the second cover structure are different from the first embodiment.

As shown in FIG. 4, the first half cylinder 21 has a first joint surface 211, the second half cylinder 22 has a second joint surface 221, and the first joint surface 211 and the second joint surface 221 correspond to each other.

In this embodiment, one of the first junction 211 of the first half cylinder 21 and the second junction 221 of the second cylinder 22 have an L-shaped structure, respectively, and when the first half cylinder 21 is When the second half cylinders 22 are assembled into a hollow cylinder, the first cylinders 21 and the second cylinders 22 are fitted to each other by an L-shaped structure.

On the other hand, the other first connecting surface 211 of the first half cylinder 21 is provided with a plurality of jacks 2111, and the other second connecting surface 221 of the second half cylinder 22 is provided with a plurality of pins 2211, and the second connecting surface The pin 221 on the 221 is corresponding to the jack 2111 of the first joint 211, and when the first half cylinder 21 and the second half cylinder 22 are assembled into a hollow cylinder, the pin 2211 is sheathed at the jack. 2111 is shown in FIG. 4 and FIG. 5(A).

In addition, a sealing layer 261 is further disposed on the first connecting surface 211 of the first half cylinder 21, and the first connecting surface 211 and the second connecting surface 221 are better adhered through the sealing layer 261.

In this embodiment, the first half cylinder 21 and the second half cylinder 22 are joined in two different ways (L-shaped structure, and pins and jacks) to form a hollow cylinder, as shown in FIG. 4 and FIG. 5 . (A) is shown. However, the joint faces of the first half cylinder 21 and the second half cylinder 22 can also be joined in the same manner to be assembled into a hollow cylinder.

On the other hand, in the tissue engineering device of the present embodiment, the first cover 23 and the second cover 24 are respectively provided with a ball bearing 244 (here, the ball bearing of the first cover 23 is not shown), wherein the ball bearing The 244 includes: an outer ring 2441, an inner ring 2442, and a plurality of balls 2443. The ball 2443 is disposed between the outer ring 2441 and the inner ring 2442, and the outer ring 2441 of the ball bearing 244 is used as a close portion. The card is placed in the close recess 253. When the tissue engineering device of the embodiment is assembled on a body to be observed, the ball bearing 244 can fix the relative positions of the first through hole 231 and the second through hole 241 and the blood vessel to prevent the hollow cylinder from moving due to the struggle of the object to be observed. And twist the blood vessels of the subject to be observed. In addition, the first cover 23 has a first through hole 231, and the second cover 24 has a second through hole 241. Therefore, the tissue engineering device of the embodiment can pass only one blood vessel.

In addition to the use of an L-shaped structure, and a pin and a jack, the first half cylinder 21 and the second half cylinder 22 may be combined in other ways as described below.

As shown in FIG. 5(B), the first joint surface 211 of the first half cylinder 21 and the second joint surface 221 of the second half cylinder 22 respectively have arc-shaped structures corresponding to each other.

As shown in FIG. 5(C), the first junction 211 of the first half cylinder 21 and the second junction 221 of the second cylinder 22 are respectively provided with a magnet 2112, 2212, and the first half cylinder 21 and the second The half cylinders 22 are coupled to each other by the magnetic forces of the magnets 2112, 2212.

As shown in FIG. 5(D), the outer wall of the first half cylinder 21 is provided with a flange 213. The outer wall of the second half cylinder 22 is provided with a hook member 223, and is fastened by the hook member 223 and the flange 213. The first half cylinder 21 and the second half cylinder 22 are grouped into the hollow cylinder (not shown).

Example 3

Figure 6 is a schematic view of the tissue engineering apparatus of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the first embodiment except that the first half cylinder and the second half cylinder are combined, and the first cover and the second cover structure are different from the first embodiment. Here, only the first cover portion of the tissue engineering device will be described in detail.

As shown in FIG. 6, the tissue engineering device of the present embodiment further includes a lock member 27 which is attached to the first cover body 23. The first end portion 212 of the first coupling hole 254 is disposed on the first half cylinder 21, and the second coupling hole 254 is second. The portion 222 is provided on the second half cylinder 22. Therefore, when the first cover body 23 is assembled to the hollow cylinder 25, the lock member 27 is inserted into the first joint hole 254, and the lock member 27 can be fixed in the first joint hole 254 by the rotary lock member 27, The first cover 23 is fixedly assembled to the hollow cylinder 25. By the latching member 27 being inserted into the first coupling hole 254, the first half cylinder 21 and the second half cylinder 22 can be prevented from separating. In the present embodiment, the locking portion 271 of the lock member 27 corresponds to the shape of the first coupling hole 254 and assumes an I shape.

In addition, in the tissue engineering device of the embodiment, the first cover 23 has a first through hole 231, and the second cover (not shown) also has a second through hole corresponding to the first cover 23 ( Not shown in the drawings, the tissue engineering device of the present embodiment can pass only one blood vessel.

In the tissue engineering device of the present embodiment, since the first cover body and the second cover body are disposed in the same manner in the hollow cylindrical body, only the first cover body is disposed at the first end of the hollow cylindrical body. situation.

Example 4

As shown in FIG. 7, this is a schematic diagram of the tissue engineering apparatus of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the third embodiment, and the locking portion 271 of the lock member 27 is S-shaped.

Example 5

Fig. 8(A) is a partial schematic view showing the tissue engineering device provided with the space adjusting device of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of Embodiment 2, except that the tissue engineering device of the present embodiment is further provided with a space adjusting device.

As shown in FIG. 8(A), in the tissue engineering apparatus of the present embodiment, a balloon 28 is disposed in the first through hole 231 of the first cover 23. In addition, the first cover body 23 is further provided with an inflation valve 281 and a deflation valve 282, wherein the inflation valve 281 is connected to the balloon 28 through an inflation tube 283 to pass gas into the balloon 28 to expand the balloon 28; The deflation valve 282 is coupled to the balloon 28 through a deflation tube 284, and excess gas is expelled from the balloon 28 by a deflation tube 284.

Therefore, in the tissue engineering apparatus of the present embodiment, the hole area of the first through hole 231 can be adjusted by the collapse or expansion of the balloon 28. Therefore, when the tissue engineering device of the embodiment is installed on a body to be observed, since the balloon 28 can adjust the hole area of the first hole 231, the first hole 231 through which the blood vessel 101 passes can be more encrypted. Improve the sealing of tissue engineering equipment.

In the tissue engineering apparatus of the present embodiment, since the first cover body and the second cover body are provided with the same space adjustment device, only the space adjustment device disposed on the first cover body will be described herein.

Example 6

Fig. 8(B) is a partial schematic view showing the tissue engineering device provided with the space adjusting device of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of Embodiment 5, except that the space conditioning device of the present embodiment includes: a balloon, and a charger device.

As shown in FIG. 8(B), in the tissue engineering apparatus of the present embodiment, a balloon 28 is disposed in the first through hole 231 of the first cover 23. In addition, the tissue engineering device of the present embodiment further includes an inflator 285 that connects the balloon 28 through an inflation tube 283, and the inflator 285 inputs a gas into the balloon 28 to inflate the balloon 28. In addition, the space adjustment device further includes a barometer 286 coupled to the inflator 285 to adjust the gas flow rate, volume, or pressure input to the balloon 28.

In the tissue engineering device of the embodiment, since the first perforation of the first cover body and the second perforation of the second cover body are provided with the same space adjustment device, only the first cover body is disposed herein. Space adjustment device. In addition, the balloons provided in the first perforation and the second perforation can be inflated using the same charger device.

Example 7

Figure 9 is a schematic illustration of a tissue engineering device incorporating a circulation device of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of Embodiment 2, except that the tissue engineering device of the present embodiment is further provided with a circulation device.

As shown in FIG. 9, the tissue engineering device of the present embodiment further includes a circulation device, including: a motor 30, including an output portion 301, and a capturing portion 302; a first fixing member 31, which is assembled On the skin 103 of the main body 10 to be observed; a second fixing member 32 is disposed on the skin 103 of the main body 10 to be observed; a first conveying tube 303 has two ends respectively connected to the output portion 301 and the first fixing portion The element 31 is connected; and a second duct 304 is connected at its two ends to the scooping portion 302 and the second fixing member 32, respectively. Here, the first fixing member 31 can be fixed to the surface of the skin 103 of the body 10 to be observed and the tissue 102 through the suture 311 or the pin 312; and the second fixing member 32 can also be in the same manner as the first fixing member 31. (The suture 321 or the pin 322) is fixed to the body 10 to be observed.

The wall of the hollow cylinder 25 has a fluid input port 255 and a fluid output port 256, and the hollow cylinder 25 further includes a fluid input pipe 257 and a fluid output pipe 258. The two ends of the fluid input pipe 257 are respectively connected to the fluid input port 255 and the first conveying pipe 303 connected to the first fixing member 31. The two ends of the fluid output pipe 258 are respectively connected to the fluid output port 258 and the second fixed pipe. The second delivery tube 304 of element 32 is connected. Therefore, in the present embodiment, the fluid is outputted through the output portion 301 of the motor 30, and the pumping portion 302 draws the fluid, so that the flow system can sequentially flow through the first delivery tube 303, the fluid input tube 257, the hollow cylinder 25, and the fluid. An output tube 258 and a second delivery tube 304. Thereby, the purpose of gas or liquid exchange can be achieved.

By providing this circulation device, the tissue engineering device of the present embodiment can be used in various studies affecting angiogenesis factors such as growth factor regulation, oxygen/carbon dioxide exchange, and hyper/hypobaric oxygen therapy. In addition, if a heater and a negative feedback temperature sensor are added to the tissue engineering device of the embodiment, the temperature can be adjusted, or used to investigate the influence of temperature on blood vessels.

Example 8

Figure 10 is a schematic illustration of the tissue engineering device of the present embodiment. The tissue engineering device of the embodiment is similar to the tissue engineering device of the second embodiment, except that the tissue engineering device of the embodiment further comprises a hollow inner cylinder.

As shown in FIG. 10, the tissue engineering device of the present embodiment further includes a first inner half cylinder 215 and a second inner half cylinder 225. The first inner half cylinder 215 and the second inner half cylinder 225 are coupled to each other. The group is set as a hollow inner cylinder. In the present embodiment, the first inner half cylinder 215 corresponds to the first half cylinder 21 and is disposed inside the first half cylinder 21 , and the second inner half cylinder 225 corresponds to the second half cylinder 22 and is disposed at the second The inside of the half cylinder 22 is. Therefore, when the first half cylinder 21 and the second half cylinder 22 are assembled into a hollow cylinder, and the first inner cylinder 215 and the second inner cylinder 225 are assembled into a hollow inner cylinder, the hollow inner cylinder is located in the hollow cylinder. in vivo.

Further, in the tissue engineering apparatus of the present embodiment, the hollow cylinders (ie, the first half cylinder 21 and the second cylinder 22) and the hollow inner cylinder (ie, the first inner cylinder 215 and the second inner cylinder 225) The pipe wall is provided with a plurality of holes 214, 224, 2151, 2251, and a space formed by the hollow cylinder and the hollow inner cylinder is provided with a permeable membrane 40. Further, the permeable membrane 40 disposed between the hollow cylinder and the hollow inner cylinder is replaceable.

Example 9

Figure 11 is a schematic illustration of the tissue engineering device of the present embodiment. The tissue engineering device of the present embodiment is similar to the tissue engineering device of the first embodiment, except that the tissue engineering device of the present embodiment further includes a plurality of permeable membranes 50 which are radially fixed in the hollow cylinder 25. At the same time, the permeable membrane 50 also has a third perforation 501 to allow passage of blood vessels of the subject to be observed.

In more detail, in the tissue engineering device of the present embodiment, the inner wall of the hollow cylinder 25 is provided with a fixing groove 259, and the permeable membrane 50 is radially fixed in the fixing groove 259. When the first half cylinder 21 and the second half cylinder 22 are assembled into a hollow cylinder, the permeable membrane 50 can divide the inner space of the hollow cylinder into a plurality of small spaces. Since the permeable membrane 50 is a molecular weight selective membrane which allows molecules having a specific size to pass through, a selective release of the substance can be achieved by placing different substances in a small space into which the hollow cylinder is divided.

Example 10

Figure 12 is a schematic illustration of the tissue engineering device of the present embodiment. The tissue engineering device of the present embodiment is similar to the tissue engineering device of the embodiment 9, except that the tissue engineering device of the present embodiment further includes a plurality of spacers 60 which are radially fixed to the hollow cylinder 25 in the same manner as the permeable membrane 50. Inside. Further, the partition 60 further has a fourth through hole 601 to pass the blood vessel of the body to be observed. Furthermore, the partition 60 further has a plurality of through holes 602 extending through the partition 60.

As shown in FIG. 12, the tissue engineering device of the present embodiment is provided with a permeable membrane 50 and a partition 60 in the axial direction of the first cover 23 or the second cover 24, and the hollow cylinder internal space can be Divided into several small spaces. Here, it is preferable to place the substance to be studied in the space formed by the permeable membrane 50 and the partition 60, and the substance to be studied can be transported to the space formed by the two partitions 60 by the fourth perforation 601 of the partition 60. However, the use of the molecular weight selective permeable membrane 50 prevents the substance to be studied from being transferred into the space formed by the permeable membrane 50 and the first cover 23 or the second cover 24.

In addition, in order to make the space separated by the permeable membrane 50 and the partition 60 have a sealing property, a space adjusting device may be selectively disposed in the third through hole 501 and the fourth through hole 601, as shown in FIG. 8(A) or FIG. 8 ( B) is shown.

Example 11

Figure 13 is a schematic view showing the first end of the hollow cylinder of the tissue engineering apparatus of the present embodiment. The tissue engineering device of the embodiment is similar to the tissue engineering device of the embodiment 9, except that the first end and the second end (not shown) of the hollow cylinder of the tissue engineering device of the embodiment are respectively provided with a slope 2511. A protective layer 70 is disposed on the slope 2511, and the material of the protective layer 70 is silicone.

Since the first end and the second end of the hollow cylinder of the tissue engineering device of the embodiment are provided with a slope and a protective layer, the blood vessel can be protected from being damaged by the edge of the hollow cylinder.

Example 12

Figure 14 is a schematic illustration of the tissue engineering device of the present embodiment. The tissue engineering device of the embodiment is similar to the tissue engineering device of the second embodiment, except that the tissue engineering device of the embodiment further includes a plurality of fixing members 70, which are disposed on the hollow cylinder 25, and the hollow cylinder 25 is fixed through Element 70 is attached to the body to be viewed (not shown). In more detail, one end of the fixing member 70 is disposed on the outer side of the hollow cylinder 25, and the other end of the fixing member 70 is provided with a fixing hole 701; and a fixing thread 701 is passed through a fixing thread (not shown). The fixing member 70 can be fixed to the body to be observed (not shown).

Example 13

Figure 15 is a schematic illustration of the tissue engineering device of the present embodiment. The tissue engineering device of this embodiment is similar to the tissue engineering device of the first embodiment, except that the hollow cylinder system of the tissue engineering device of the present embodiment is square, and a partition 60 is provided at the bottom of the hollow cylinder.

Therefore, when the tissue engineering device of the embodiment is installed on the main body to be observed, the space above the partition 60 is provided with the substance to be studied, and the space below the partition 60 is used for placing the blood vessel of the main body to be observed, and the substance to be studied The through hole 602 of the partition 60 is inserted into the space in which the blood vessel is placed (i.e., the space below the partition 60).

The above-mentioned embodiments are merely examples for convenience of description, and the scope of the claims is intended to be limited to the above embodiments.

10. . . Subject to be observed

101. . . Blood vessel

102. . . organization

103. . . skin

11. . . Rubber hose

111. . . Suture

112. . . Hollow hole

twenty one. . . First half cylinder

211. . . First junction

2111. . . Jack

2112,2212. . . magnet

212. . . First

213. . . Flange

214,224,2151,2251. . . Hole

215. . . First inner half cylinder

twenty two. . . Second half cylinder

221. . . Second junction

2211. . . Pin

222. . . Second part

223. . . Buckle hook

225. . . Second inner half cylinder

twenty three. . . First cover

231. . . First perforation

232. . . Flange

233. . . Closed part

twenty four. . . Second cover

241. . . Second perforation

242. . . Flange

243. . . Closed part

244. . . Ball bearing

2441. . . Outer ring

2442. . . Inner ring

2443. . . Ball

25. . . Hollow cylinder

251. . . First opening

2511. . . Bevel

252. . . Second opening

253. . . Closed groove

254. . . First bonding hole

255. . . Fluid input port

256. . . Fluid outlet

257. . . Fluid input tube

258. . . Fluid output tube

259. . . Fixed groove

261. . . Sealing layer

27. . . Lock

271. . . Carding department

28. . . balloon

281. . . Inflation valve

282. . . Vent valve

283. . . Inflatable tube

284. . . Venting pipe

285. . . Inflator

286. . . Barometer

29. . . Hinge

30. . . motor

301. . . Output department

302. . . Capture department

303. . . First duct

304. . . Second duct

31. . . First fixing element

311. . . Suture

312. . . Pin

32. . . Second fixing element

321. . . Suture

322. . . Pin

50. . . Osmotic membrane

501. . . Third perforation

60. . . Partition

601. . . Fourth perforation

602. . . Through hole

70. . . Fixed component

701. . . Fixed hole

80. . . The protective layer

Figure 1 is a conventional tissue engineering device.

2 is a schematic view showing the tissue engineering device of Embodiment 1 of the present invention installed on a main body to be observed.

3A is an exploded perspective view showing a first cover body and a second cover body of the tissue engineering device according to Embodiment 1 of the present invention.

3B is a schematic view showing one side of a first half cylinder and a second half cylinder of the tissue engineering apparatus according to Embodiment 1 of the present invention.

3C is a schematic view showing the other side of the first half cylinder and the second half cylinder of the tissue engineering apparatus according to Embodiment 1 of the present invention.

Fig. 4 is a schematic view showing a tissue engineering apparatus according to a second embodiment of the present invention.

Figure 5 is a schematic view showing the combination of the first half cylinder and the second half cylinder of the tissue engineering device of the present invention.

Fig. 6 is a schematic view showing the first cover body of the tissue engineering device of the third embodiment of the present invention assembled in a hollow cylinder;

Fig. 7 is a partial schematic view showing the first cover body of the tissue engineering device of the fourth embodiment of the present invention assembled in a hollow cylinder; .

Fig. 8(A) is a partial schematic view showing a tissue engineering apparatus provided with a space adjusting device according to a fifth embodiment of the present invention.

Fig. 8 (B) is a partial schematic view showing a tissue engineering device provided with a space adjusting device according to a sixth embodiment of the present invention.

Figure 9 is a schematic illustration of a tissue engineering apparatus incorporating a circulation device in accordance with a seventh embodiment of the present invention.

Figure 10 is a schematic view of a tissue engineering apparatus according to Embodiment 8 of the present invention.

Figure 11 is a schematic illustration of a tissue engineering device of Example 9 of the present invention.

Figure 12 is a schematic illustration of a tissue engineering apparatus in accordance with a tenth embodiment of the present invention.

Figure 13 is a schematic view showing the first end of the hollow cylinder of the tissue engineering apparatus of the eleventh embodiment of the present invention.

Figure 14 is a schematic view showing a tissue engineering apparatus of Embodiment 12 of the present invention.

Figure 15 is a schematic illustration of a tissue engineering device of Example 13 of the present invention.

101. . . Blood vessel

twenty one. . . First half cylinder

twenty two. . . Second half cylinder

twenty three. . . First cover

231. . . First perforation

232. . . Flange

233. . . Closed part

twenty four. . . Second cover

241. . . Second perforation

242. . . Flange

243. . . Closed part

25. . . Hollow cylinder

251. . . First opening

252. . . Second opening

253. . . Closed groove

Claims (21)

A tissue engineering device comprising: a first half cylinder; a second half cylinder combined with the first half cylinder to form a hollow cylinder, the first end of the hollow cylinder having a first Opening, and the second end of the hollow cylinder has a second opening; a first cover body is disposed on the first end of the hollow cylinder and corresponds to the first opening, through the first cover Opening and closing the first opening, and the first cover has at least one first through hole; and a second cover disposed on the second end of the hollow cylinder and correspondingly a second opening through which the second cover is opened or closed to open or close the second opening, and the second cover has at least one second through hole; wherein, when the tissue engineering device is assembled on a body to be observed The blood vessel of the body to be observed sequentially passes through the first perforation of the first cover, the hollow cylinder, and the second perforation of the second cover. The tissue engineering device of claim 1, wherein the first perforation of the first cover and the second perforation of the second cover are respectively provided with a balloon, and by the balloon Collapse or expansion to adjust the hole area of the first perforation and the second perforation. The tissue engineering device of claim 2, wherein the first cover and the second cover are further provided with an inflation valve and a deflation valve, the inflation valve connecting the balloon to expand the balloon And the vent valve connects the balloon to collapse the balloon. The tissue engineering device of claim 2, further comprising an inflator for connecting the balloon, the inflator being inserted into the balloon of a gas to inflate the balloon. The tissue engineering device of claim 1, wherein the first half cylinder has a first junction, the second cylinder has a second junction, and the first junction and the second The junctions correspond to each other. The tissue engineering device of claim 5, wherein the first junction of the first half cylinder and the second junction of the second cylinder have an L-shaped structure, and the first The half cylinder and the L-shaped structure of the second cylinder are fitted to each other. The tissue engineering device of claim 5, wherein the first junction of the first half cylinder is provided with a plurality of sockets, and the second junction of the second cylinder is provided with a plurality of pins The pins are corresponding to the jacks, and when the first half cylinder and the second half cylinder are assembled into the hollow cylinder, the pins are inserted into the sockets. The tissue engineering device of claim 5, wherein the first junction of the first half cylinder is provided with a sealing layer. The tissue engineering device of claim 5, wherein the first junction of the first half cylinder and the second junction of the second cylinder are respectively provided with a magnet, and the first half cylinder The second half cylinder is coupled to each other through the magnets. The tissue engineering device of claim 1, wherein the outer wall of the first half cylinder is provided with a flange, and the outer wall of the second cylinder is provided with a hook member, and the hook member is The flanges are hooked to each other to form the first half cylinder and the second half cylinder into the hollow cylinder. The tissue engineering device of claim 1, further comprising a lock member, wherein the first end and the second end of the hollow cylinder are respectively provided with a first coupling hole, the first a first portion of the first coupling hole is disposed on the first half cylinder, and the second portion of the first coupling hole is disposed on the second half cylinder, and the first cover body and the second cover body When the hollow cylinder is assembled, the locking member is inserted into the first coupling hole to fix the first cover and the second cover to the hollow cylinder. The tissue engineering device of claim 1, wherein the first end and the second end of the hollow cylinder are respectively provided with a close groove, and the first cover and the second cover are respectively provided There is a sealing portion, and when the first cover body and the second cover body are assembled to the hollow cylindrical body, the closely fitting portions are stuck in the close fitting grooves. The tissue engineering device of claim 1, wherein the first cover body and the second cover system are respectively provided with a ball bearing. The tissue engineering device of claim 1, wherein the first end and the second end of the hollow cylinder are respectively provided with a slope. The tissue engineering device of claim 14, wherein the slope is provided with a protective layer. The tissue engineering device of claim 1, further comprising a circulation device, the cycle device comprising: a motor comprising an output portion and a picking portion; a first fixing member, the first fixing member a second fixing member is disposed on the main body to be observed; a first conveying pipe has two ends connected to the output portion and the first fixing member respectively; and a second conveying a tube, the two ends of which are respectively connected to the capturing portion and the second fixing member; wherein the wall of the hollow cylinder has a fluid input port and a fluid output port, and the hollow tube further comprises a fluid An input tube and a fluid output tube, the two ends of the fluid input tube are respectively connected to the fluid input port and the first conveying tube connected to the first fixing element, and the two ends of the fluid output tube are respectively The fluid outlet is connected to the second conveying pipe connected to the second fixing element, and the output portion of the motor outputs a fluid, and the capturing portion sucks the fluid, so that the flow system sequentially flows through the first conveying pipe. The fluid An input tube, the hollow cylinder, the fluid output tube, and the second delivery tube. The tissue engineering device of claim 1, further comprising a first inner half cylinder and a second inner half cylinder, wherein the first inner half cylinder and the second inner half cylinder are combined with each other Forming a hollow inner cylinder, and the hollow inner cylinder is located in the hollow cylinder. The tissue engineering device of claim 17, wherein the hollow cylinder and the wall of the hollow inner cylinder are provided with a plurality of holes, and the hollow cylinder and the hollow inner cylinder form a space system There is a permeable membrane. The tissue engineering device according to claim 1, further comprising a plurality of permeable membranes radially fixed in the hollow cylinder. The tissue engineering device of claim 1, further comprising a plurality of separators and a plurality of permeable membranes, wherein the separators and the permeable membranes are radially fixed in the hollow cylinder. The tissue engineering device of claim 1, further comprising a plurality of fixing elements disposed on the hollow cylinder, and the hollow cylinder system is fixed to the body to be observed through the fixing elements.