201224425 六、發明說明： 【發明所屬之技術領域】 本發明是有關於一種承載裝置（carrying device)及在此承載裝置 中容置檢體（specimen)的方法，且特別是有關於一種供檢視裝置 (inspection device)檢視檢體用的承載裝置及在此承載裝置中容置檢 體的方法。 【先前技術】 健康檢查（health examination)為現代人為了檢視自身健康狀況時 所絰$使用的一種方法。其中，對受檢者（examinee)採集血液或精液 等檢體’並且透過檢視裝置對檢體進行統計、分析及檢驗即為健康檢 查時所不可或缺的步驟之一。 舉例來說，醫療機構（medical institution)或檢驗機構 ms—)可紐由受檢者身上所採翻的精液放置於載玻片（齡） 與蓋玻片（_rglass)之間。然'後，檢驗人員即可藉由掃描器、顯微 鏡（microscope)、具有感光麵合元件（chargec〇upleddevice,ccD)的 =鏡戦是魏具錢似魏的檢财絲計算定量精液中的精子 ^ °其中，精液量可藉由精液與蓋玻片的接觸面積以及载玻片與蓋 玻片之間的間隙來估算出。 然而，-般的載玻片與蓋玻片之間跡容易轉固定的間隙，進 :導致檢驗人員難_確地計算出檢體_f。如此201224425 VI. Description of the Invention: [Technical Field] The present invention relates to a carrying device and a method for accommodating a specimen therein, and more particularly to a viewing device (inspection device) A carrier device for inspecting a sample and a method of accommodating the sample in the carrier device. [Prior Art] A health examination is a method used by modern people to examine their own health conditions. Among them, the collection of blood or semen samples from the examinee and the statistical analysis, analysis and inspection of the specimen through the inspection device are one of the indispensable steps in the health check. For example, a medical institution or a testing institution ms-) can be placed between a slide (age) and a cover glass (_rglass). However, the inspector can calculate the sperm in the quantitative semen by means of a scanner, a microscope, a chargec〇upled device (ccD), a mirror, and a Weishui-like Weis. ^ ° where the amount of semen can be estimated by the contact area of the semen with the coverslip and the gap between the slide and the coverslip. However, the gap between the slide and the cover slip is easy to turn into a fixed gap, which makes it difficult for the examiner to calculate the sample _f. in this way
^驗。為了改善上述問題，先前技術提出了 IS 片\^載玻片或盍玻片的周緣形成有，厚度的凸起。因此，蓋玻 片覆盍載玻片時可與載玻片維翻定的嶋:。 盍玻 值得注意的是，此種承餘置較為昂貴，而且載玻片與 間的間隙並無法根據使用需求調整。 片之 201224425 【發明内容】 為了解決上述問題，本發明提供一種承載裝置，其載玻片與蓋玻 片之間的間隙可才艮據使用需求調整。 為了解決上述問題，本發明更提供一種在承載裝置中容置檢體的 方法，其所使用的承载裝置較為便宜。 本發明提供一種承載裝置，用以承載一檢體，以供一檢視裝置檢 視檢體’其中承載裝置包括一載玻片、多個介質（medium)、一蓋玻片 以及多個間隙物（spacer)。載玻片用以承載檢體，並且具有一用以供 檢視裝置檢視檢體的檢視區域（inspection regi〇n )以及一環繞於 (surround )檢視區域的周邊區域（peripherai regi〇n )，其中檢體適於 佈滿檢視區域。這些介質配置於載玻片上，位於周邊區域中，並且環 繞於檢視區域。蓋玻片配置於載玻片上，用以覆蓋檢體，並且覆蓋檢 視區域、至少部份的周邊區域與這些介質。這些間隙物透過這些介質 保持於載玻片上，並且載玻片透過這些間隙物承載蓋玻片。 在本發明的一實施例中’上述的間隙物預先混合於這些介質中， 並且與這些介質一併配置於載玻片上。 本發明更提供一種在一承載裝置中容置一檢體的方法，其包括下 列步驟。首先’提供一載玻片’其具有一用以供一檢視裝置檢視檢體 的檢視區域以及一環繞於檢視區域的周邊區域。接著，在載玻片上提 供檢體，以使檢體佈滿檢視區域。之後，在載玻片上的周邊區域中配 置多個介質以及多個間隙物，以使這些介質與這些間隙物環繞於檢 體’其中這些間隙物透過這些介質保持於載玻片上。然後，提供一蓋 玻片，以覆蓋檢體、檢視區域、至少部份的周邊區域、這些介質與這 些間隙物，以使載玻片透過這些間隙物承載蓋玻片。 在本發明的一實施例中，在上述的載玻片上的周邊區域中配置這 201224425 些介質以及這些間隙物的步驟包括下列步驟。先將這些間隙物混合於 這些介質中，然後再在載玻片上配置混合有這些間隙物的這些介質。 在本發明的一實施例中，在上述的載玻片上的周邊區域中配置這 - 些介質以及這些間隙物的步驟包括下列步驟。先在載玻片上配置這些 介質，然後再分別在這些介質上散佈這些間隙物。 在本發明的一實施例中’上述的間隙物、介質與檢體之間不互溶 (immiscibly )，亦不產生化學反應（inactively )。 在本發明的一實施例中，上述的介質的顏色不同於檢體的顏色。 0 在本發明的一實施例中，上述的間隙物呈球狀。 在本發明的一實施例中，上述的介質呈液態（liquid)或膠狀 (jelly) 〇 基於上述，在本發明中，蓋玻片與載玻片透過間隙物維持固定的 間隙。因此，承載裝置可使用未經過特殊處理的蓋玻片與載玻片以 降低其整體製作成本^且，蓋玻片與載玻片之__可藉由使用 不同尺寸的間隙物來調整。 為讓本發明的上述特徵和優點能更明顯易懂，下文特舉多個實施 鲁 例’並配合所附圖式，作詳細說明如下。 【實施方式】 —，1 本發明—實施例的—種容置有—檢體的承載裝置的結 構示意圖’而圖2綠示_ i _視圖。為了能更清楚地繪示出各構 件之間的相對位置及連接義，圖丨細2放大了载玻狀蓋玻片的 居度，並且放大了間隙物的尺寸。 月參考圖1與圖2所示，承載裝置⑽包括一載玻片ιι〇、多個介 質120 ® 1與圖2中示意地緣示出二個）、一蓋玻片13〇以及多個間 201224425 隙物140 (圖1與圖2中示意地繪示出四個）。載玻片no用以承載一 檢體200’並且具有一可供一檢視裝置3〇〇檢視的檢視區域U2以及一 . 環繞於檢視區域112的周邊區域114。於此實施例中，檢體200例如是 血液 '精液、尿液或是其他待檢測物，其佈滿檢視區域112，並且可溢 入部份的周邊區域114中。另外，檢視裝置30〇則可以是顯微鏡、具 有感光耦合元件的攝影鏡頭或者是其他可用以檢視容置於承載裝置 100中的檢體200的檢視裝置。 這些介質120可以是液態介質或者是膠狀介質，其配置於載玻片 110上，位於周邊區域114中，並且環繞於檢視區域112。而且，這些 # 介質120不與檢體200接觸。此外，這些間隙物140可以是球狀間隙 物，並且可預先混合於這些介質12〇中。因此，使用者將這些介質12〇 配置於載玻片110上時，即可一併將這些間隙物14〇配置於載玻片n〇 上。此時’這些間隙物140即會透過這些介質120保持於載玻片110 上。或者，使用者亦可先將這些介質120配置於載玻片11〇上，然後 再分別在這些介質120上散佈這些間隙物140,以使這些間隙物14〇混 合於這些介質120中。如此一來，這些間隙物140亦可透過這些介質 120保持於載玻片11〇上。 除此之外，這些介質120還可以是黏著劑。此時，使用者可先將 • 這些介質12〇配置於載玻片110或蓋玻片130上，然後再分別在這些 介質120上散佈這些間隙物14〇,以使這些間隙物140附著於這些介質 120上。如此一來’這些間隙物140亦可透過這些介質ι2〇保持於載玻 片110或蓋玻片130上。 然後’使用者可再將蓋玻片130覆蓋於載玻片11〇之上，以覆蓋 檢視區域112、至少部份的周邊區域114、這些介質120與這些間隙物 140，進而將檢體200保持於載玻片110與蓋玻片130之間。此時，載 玻片110即會透過這些間隙物140承載蓋玻片130,進而與蓋玻片I% 之間維持一定的間隙。 201224425 相較於先前技術，在本發明中，載玻片11〇與蓋玻片13〇並不需 要經過特殊處理，而且介質120與間隙物140可選用水及液晶面板製 权中所使用的間隙物等較為便宜的材料，因此承載裝置1〇〇的整體製 作成本會較低。而且，使用者只需以檢視區域112的面積乘上間隙物 140的尺寸，即可精確地計算出檢視區域112中的檢體2〇〇的總量。另 外，使用者還可根據其使用需求使用不同尺寸的間隙物14〇，以調整載 玻片110與蓋玻片13〇之間的間隙。 除此之外’為了避免介質120及間隙物14〇接觸到檢體2〇〇之後 會影響到檢視結果的準確性。使用者可選用不會與檢體2〇〇互溶，亦 不會與檢體200產生化學反應的介質12〇及間隙物14〇。另外，使用者 更可選用顏色不同於檢體2〇〇顏色的介質120,以利辨識出檢體2〇〇的 外輪廓。 圖3繪示出本發明一實施例的一種在承載裝置中容置檢體的流程 圖。凊參考圖1、圖2與圖3所示，使用者可根據下列步驟將檢體2〇〇 容置於承載裝置1〇〇中。 。首先，將檢體200配置於載玻片11〇上，以使檢體2〇〇佈滿檢視 區域112,並將介質12〇與間隙物14〇配置於載玻片11〇上的周邊區域 114中，以環繞於檢體2〇〇 (si〇〇)。於此實施例中，這些間隙物14〇 可預先混合於這些介質120中，以與這些介質12〇 一併配置於載玻片^ Test. In order to improve the above problems, the prior art proposes that the periphery of the IS sheet or the glass slide is formed with a projection of a thickness. Therefore, when the cover glass covers the slide, it can be adjusted with the slide. It is worth noting that such a residual space is expensive and the gap between the slides and the slide cannot be adjusted according to the needs of use. SUMMARY OF THE INVENTION In order to solve the above problems, the present invention provides a carrying device in which the gap between the slide glass and the cover glass can be adjusted according to the use requirements. In order to solve the above problems, the present invention further provides a method of accommodating a specimen in a carrying device, which uses a carrier device which is relatively inexpensive. The invention provides a carrying device for carrying a sample for an inspection device to inspect a sample, wherein the carrying device comprises a slide, a plurality of medium, a cover glass and a plurality of spacers (spacer ). The slide is used to carry the specimen, and has an inspection area (inspection regi〇n) for the inspection device to view the specimen and a peripheral region (peripherai regi〇n) surrounding the inspection area. The body is suitable for covering the viewing area. These media are placed on the slide, in the peripheral area, and around the viewing area. The coverslip is disposed on the slide to cover the sample and cover the inspection area, at least a portion of the peripheral area, and the medium. These spacers are held on the slide through these media, and the slides carry the coverslip through these spacers. In an embodiment of the invention, the spacers described above are premixed in the medium and disposed on the slide together with the medium. The present invention further provides a method of accommodating a sample in a carrier device comprising the following steps. First, a "slide" is provided which has a viewing area for viewing the sample by a viewing device and a peripheral area surrounding the viewing area. Next, a specimen is provided on the slide so that the specimen fills the inspection area. Thereafter, a plurality of media and a plurality of spacers are disposed in the peripheral region on the slide such that the media and the spacers surround the sample' wherein the spacers are retained on the slide through the media. A cover glass is then provided to cover the specimen, the viewing area, at least a portion of the peripheral area, the media and the interstitials such that the slides carry the coverslip through the interstitials. In an embodiment of the invention, the step of arranging the 201224425 media and the spacers in the peripheral region on the slide described above includes the following steps. These spacers are first mixed into these media, and then these media mixed with these spacers are placed on the slide. In an embodiment of the invention, the step of disposing the media and the spacers in the peripheral region on the slide described above comprises the following steps. These media are first placed on the slides and then spread on these media separately. In an embodiment of the invention, the spacer, the medium, and the sample are immiscibly and do not produce an inactively. In an embodiment of the invention, the color of the medium is different from the color of the sample. In an embodiment of the invention, the spacer is spherical. In an embodiment of the invention, the medium is in a liquid or jelly. Based on the above, in the present invention, the cover glass and the slide are maintained in a fixed gap through the spacer. Therefore, the carrier device can use the cover glass and the slide without special treatment to reduce the overall manufacturing cost thereof, and the cover glass and the glass slide can be adjusted by using spacers of different sizes. The above described features and advantages of the present invention will become more apparent from the following description. [Embodiment] - 1 is a schematic view of a structure of a carrier device in which a sample is accommodated, and FIG. 2 shows a view of the _i_. In order to more clearly illustrate the relative position and connection between the components, Figure 2 magnifies the occupancy of the glass cover slip and enlarges the size of the spacer. Referring to FIG. 1 and FIG. 2, the carrying device (10) includes a slide ιι, a plurality of media 120 ® 1 and two schematically shown in FIG. 2 , a cover glass 13 〇 and a plurality of 201224425 Gap 140 (four are schematically depicted in Figures 1 and 2). The slide no is used to carry a sample 200' and has a view area U2 for viewing by the view device 3 and a peripheral area 114 surrounding the view area 112. In this embodiment, the sample 200 is, for example, blood 'semen, urine, or other object to be detected, which is covered with the inspection area 112 and can overflow into the peripheral portion 114 of the portion. Alternatively, the viewing device 30A may be a microscope, a photographic lens having a photosensitive coupling element, or other inspection device that can be used to view the sample 200 housed in the carrier device 100. These media 120 may be liquid media or gelatinous media disposed on the slide 110, in the peripheral region 114, and surrounding the viewing region 112. Moreover, these # media 120 are not in contact with the specimen 200. Additionally, these spacers 140 can be spherical spacers and can be premixed in these media 12〇. Therefore, when the user arranges the media 12 on the slide glass 110, the spacers 14 can be disposed on the slide glass n〇. At this time, the spacers 140 are held on the slide glass 110 through the media 120. Alternatively, the user may first arrange the media 120 on the slides 11 and then spread the spacers 140 on the media 120 to mix the spacers 14 into the media 120. In this way, the spacers 140 can also be held on the slide 11 through the medium 120. In addition to these, these media 120 can also be adhesives. At this time, the user may first arrange the media 12 on the slide 110 or the cover glass 130, and then spread the spacers 14 on the media 120 respectively, so that the spacers 140 are attached to the media 140. On the medium 120. As a result, these spacers 140 can also be held on the slide glass 110 or the cover glass 130 through these media. Then the user can then cover the cover glass 130 over the slide 11 , to cover the inspection area 112, at least a portion of the peripheral area 114, the medium 120 and the spacers 140, thereby maintaining the sample 200 Between the slide glass 110 and the cover glass 130. At this time, the slide glass 110 carries the cover glass 130 through the spacers 140, thereby maintaining a certain gap with the cover glass I%. 201224425 Compared with the prior art, in the present invention, the slide 11 〇 and the cover glass 13 〇 do not need special treatment, and the medium 120 and the spacer 140 can be used for water and the gap used in the liquid crystal panel. Since the material is relatively inexpensive, the overall manufacturing cost of the carrier device 1 会 will be low. Moreover, the user can accurately calculate the total amount of the sample 2 in the inspection region 112 by multiplying the area of the inspection region 112 by the size of the spacer 140. In addition, the user can also use different sizes of spacers 14 根据 according to their use requirements to adjust the gap between the slide glass 110 and the cover glass 13 。. In addition, in order to prevent the medium 120 and the spacers 14 from coming into contact with the sample 2, the accuracy of the inspection result is affected. The user can select the medium 12 and the spacer 14 which do not interfere with the sample 2 and do not chemically react with the sample 200. In addition, the user may further select a medium 120 having a color different from that of the specimen 2 to facilitate identification of the outer contour of the specimen 2〇〇. Fig. 3 is a flow chart showing the accommodation of a sample in a carrying device according to an embodiment of the invention. Referring to Figures 1, 2 and 3, the user can place the sample 2 in the carrier device 1 according to the following steps. . First, the specimen 200 is placed on the slide glass 11 so that the specimen 2 is covered with the inspection region 112, and the medium 12 and the spacer 14 are disposed on the peripheral region 114 on the slide 11 In order to surround the specimen 2〇〇(si〇〇). In this embodiment, the spacers 14〇 may be pre-mixed in the media 120 to be disposed on the slide together with the media 12〇.
Uo上，並且可透過這些介質12〇保持於載玻片110上。或者，使用者 亦可先將這些介質120配置於載玻片ι10上，然後再分別在這些介質 120上散佈這些間隙物140，以使這些間隙物140混合於這些介質120 中。如此一來，這些間隙物140亦可透過這些介質】2〇保持於載玻片 110 上。 另外，當這些介質12〇為黏著劑時，使用者可先將這些介質12〇 配置於載玻片11〇或蓋玻片130上，然後再分別在這些介質12〇上散 佈這些間隙物140，以使這些間隙物140附著於這些介質120上。如此 201224425 一來’這些間隙物140亦可透過這些介質i2〇保持於載玻片no或蓋 玻片130上。 然後，使用者可再將蓋玻片130配置於載玻片110上，以覆蓋檢 體200、檢視區域112、至少部份的周邊區域114、這些介質12〇與這 些間隙物140 (S200)。此時，載玻片no即可透過這些間隙物14〇來 承載蓋玻片130,並且可透過這些間隙物140與蓋玻片13〇之間維持一 定的間隙。之後，使用者只需以檢視區域112的面積乘上間隙物14〇 的尺寸，即可精確地計算出檢視區域112中的檢體2〇〇的總量。而且，Uo is placed on the slide 110 through these media 12〇. Alternatively, the user may first arrange the media 120 on the slide ι10 and then spread the spacers 140 on the media 120 to mix the spacers 140 into the media 120. In this way, the spacers 140 can also be held on the slide glass 110 through the medium. In addition, when the media 12 is an adhesive, the user may first dispose the media 12 on the slide 11 or the cover glass 130, and then spread the spacers 140 on the media 12, respectively. These spacers 140 are attached to these media 120. Thus, in 201224425, these spacers 140 can also be held on the slide no or the cover glass 130 through these media i2. The user can then place the cover glass 130 on the slide 110 to cover the sample 200, the inspection area 112, at least a portion of the peripheral area 114, the media 12 and the spacers 140 (S200). At this time, the slide no can pass through the spacers 14 to carry the cover glass 130, and a certain gap can be maintained between the spacers 140 and the cover glass 13〇. Thereafter, the user simply multiplies the area of the inspection region 112 by the size of the spacer 14 , to accurately calculate the total amount of the specimen 2 in the inspection region 112. and,
使用者還可藉由使用不同尺寸的間隙物14〇來調整檢視區域112中的 檢體200的總量。 ^综合上述，在本發明中，間隙物透過介質保持於載玻片上，以使 蓋玻片與載玻片可透賴隙物轉固定關隙。因此，承載裝置可使 用未經過特殊處理的蓋玻^[與触#，崎低其整體製作成本。之後， 使用者只需以檢視區域的面積乘上間隙物的尺寸，即可精確地計算出 檢視區域中的檢體的總量。而且，蓋玻片與載玻片之間的間隙^由 使用不同尺寸的間隙物來調整。 另外’㈣者可咖不會無體互溶，亦不會與檢體產生化學反 …的介質及_物’以避免介f及___碰之齡影響到檢 2果的準確性而^，使用者更可選悲色不同於檢體顏色的介質， 以利辨識出檢體的外輪廓。 穌發明已財施例揭露如上，然其並_以限定本發明，任 内㈣者，在不麟本㈣⑽神和範圍 請專_=者=本發㈣__後附的申 201224425 【圖式簡單說明】 . 圖1繪示出本發明一實施例的一種容置有一檢體的承載裝置的結構 示意圖。 圖2繪示出圖1的俯視圖。 圖3繪示出本發明一實施例的一種在承載裝置中容置檢體的流程圖。 【主要元件符號說明】 • 100:承載裝置 110 :載玻片 112 :檢視區域 114 :周邊區域 120 :介質 130 :蓋玻片 140 :間隙物 # 200 :檢體 300 :檢視裝置 S100 ' S200 :步驟The user can also adjust the total amount of the specimen 200 in the view region 112 by using spacers 14 of different sizes. In summary, in the present invention, the spacers are held on the slide by the medium to allow the cover glass and the slide to be rotatably fixed to the gap. Therefore, the carrying device can use the cover glass that has not been specially treated, and it can reduce the overall manufacturing cost. After that, the user can accurately calculate the total amount of the sample in the inspection area by multiplying the area of the inspection area by the size of the spacer. Moreover, the gap between the cover glass and the slide is adjusted by using spacers of different sizes. In addition, '(4) can not be incompatible with each other, and will not produce a chemical anti-media with the sample and the 'object' to avoid the influence of the age of the f and the ___ touch on the accuracy of the test 2, ^ The user can select a medium with a different color than the color of the sample to identify the outer contour of the sample. He invented the financial example to expose the above, but it is _ to limit the invention, within the (four), in the non-uniform (four) (10) God and scope please _ = person = this hair (four) __ attached to the application 201224425 [simple figure BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing the structure of a carrying device for accommodating a sample according to an embodiment of the invention. Figure 2 depicts a top view of Figure 1. 3 is a flow chart of housing a sample in a carrying device according to an embodiment of the invention. [Description of main component symbols] • 100: Carrier device 110: Slide glass 112: Inspection area 114: Peripheral area 120: Media 130: Cover glass 140: Interstitial # 200: Sample 300: Inspection device S100 'S200: Step