KR101684051B1 - Apparatus for quantative analysis of biological material using information technology device - Google Patents

Abstract

The present invention relates to a biological sample quantitative analysis apparatus using an IT device. The biological sample quantitative analysis apparatus using the IT device according to the present invention is detachably attached to an IT device provided with a camera and a camera flash, and includes a case having a support, an opening for a camera and an opening for a camera flash, And includes a measurement pad containing an enzyme reaction material according to an object to be measured and reacting according to a sample to be injected for measurement.
According to the present invention, a camera and a camera flash provided in an IT device such as a smart phone are used as a light receiving unit and a light emitting unit to photograph and record the degree of color development resulting from the concentration of a sample, Analysis, and can be used in conjunction with a smartphone, so it is possible to maximize user convenience and satisfaction by not having a separate device but also analyzing the result without expert knowledge using the application of smartphone .
In addition, the present invention can be applied to various fields and diseases that can cause a color reaction by reacting with a coloring compound (4-AAP, MAOS) There is a wide range of use.

Description

TECHNICAL FIELD [0001] The present invention relates to a biological sample quantitative analysis apparatus using an IT device,

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a biological sample quantitative analysis apparatus using an IT device, and more particularly, to an apparatus for quantitatively analyzing biological samples using an IT And a biological sample quantitative analysis apparatus using the apparatus.

In general, a method for measuring the degree of disease diagnosis or state is obtained by collecting blood in the human body and analyzing the blood components. For example, in the case of diabetes, diabetes measures glucose in the blood, And the blood glucose measurement equipment provided in the hospital was used for this purpose. This has the advantage of being able to perform precise analysis, but it is not only expensive, but also has a disadvantage in that it requires technical expertise and knowledge in the measurement because of its complicated process, making it difficult for the general public to use it easily.

In recent years, in order to overcome such problems, portable diagnostic blood glucose meters which are easy to use by patients have been introduced without professional knowledge. However, this portable blood glucose meter has a disadvantage in that it has to be carried separately.

Nevertheless, in the biosensor market, blood glucose measuring devices are the majority of the market, and diabetes patients are also increasing steadily in the world. Therefore, they are portable enough to make field diagnosis, It is necessary to develop a blood glucose measuring and testing apparatus capable of measuring and quantifying blood glucose. Furthermore, there is a demand for the development of a biological sample quantitative analysis and inspection apparatus for on-site diagnosis of various biological samples used for various disease tests as well as blood glucose.

The technique which is the background of the present invention is disclosed in Korean Patent Laid-Open Publication No. 10-2014-0027735 (published on Mar. 03, 2007).

An object of the present invention is to provide a biological sample quantitative analysis apparatus using an IT device that photographs and records the degree of color development derived from the concentration of a biological sample using a camera provided in an IT device such as a smart phone .

A biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention includes a case detachably attached to an IT device having a camera and a camera flash, and having a support, a camera opening, and a camera flash opening; And a measurement pad supported by the support of the case and containing an enzyme reaction material according to an object to be measured and reacting according to a sample to be injected for the measurement.

Wherein the IT device is mounted on the case such that the camera and the camera flash are exposed through the camera opening and the camera flash opening and photographs the measurement pad through the camera and the camera flash, Can be used to analyze the measured results.

The measurement pad may include a measurement unit including an injection unit into which the sample is injected and the enzyme reaction material in which one or more substances that cause color development by reacting with the sample are mixed.

The case may further include a cover for blocking external light, and a reflection part for dispersing the light emitted from the camera flash evenly into the case may be attached to the cover.

In the case, a light splitting part for adjusting the intensity of light emitted from the camera flash may be attached to the camera flash opening.

The case may be attached to the camera opening by a right angle prism for rotating the image of the measurement pad at right angles to irradiate the camera.

The enzyme reaction material includes HRP (Horseradish peroxidase) and 4-AAP (4-aminoantipyrine), and includes GOx (glucose oxidase), AChE (Acetylcholine), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline, sodium salt (MAOS) monohydrate), ADOS (N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methoxyaniline, sodium salt, dihydrate) salt, monohydrate), ALPS (N-Ethyl-N- (3-sulfopropyl) aniline, sodium salt), DAOS (N-Eyhyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline, sodium salt ), HDAOS (N- (2-Hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline, sodium salt), MADB (N, N-Bis (N-Ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt dihydrate), TOPS (N-ethyl- N- (3- sulfopropyl) -3-methylaniline, sodium salt, monohydrate) and phenol.

A biological sample quantitative analysis apparatus using an IT device according to the present invention uses a camera and a camera flash provided in an IT device such as a smart phone as a light receiving unit and a light emitting unit to photograph and record the degree of color development derived from the concentration of the sample, By analyzing the recorded results, it is possible to perform accurate and quick analysis, and it can be used in conjunction with a smart phone, so that it is not necessary to have a separate device, and the result analysis can be performed without expert knowledge using the application of the smartphone. It is possible to maximize convenience and satisfaction.

In addition, the present invention can be applied to various fields and diseases that can cause a color reaction by reacting with a coloring compound (4-AAP, MAOS) There is a wide range of use.

In addition, the present invention requires a special external power source because it is cost-effective to manufacture an examination sheet necessary for analyzing a biological sample, that is, a measurement pad is made of paper, and operates by the capillary force inside the paper.

1 is a perspective view illustrating a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.
2 is a schematic view illustrating a measurement principle of a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.
FIG. 3 is a perspective view illustrating a right angle prism unit in a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention. FIG.
4 is a view illustrating a process of manufacturing a measurement pad in a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.
5 is a view showing the internal structure of a measurement pad in a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.
6 is a view illustrating a process of reacting a sample injected into a measurement pad in a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.
FIG. 7 is a diagram illustrating measurement results of a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.

Hereinafter, a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention will be described with reference to the accompanying drawings. In this process, the thicknesses of the lines and the sizes of the components shown in the drawings may be exaggerated for clarity and convenience of explanation.

Further, the terms described below are defined in consideration of the functions of the present invention, which may vary depending on the intention or custom of the user, the operator. Therefore, definitions of these terms should be made based on the contents throughout this specification.

FIG. 1 is a perspective view of a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention, and FIG. 2 is a view illustrating a measurement principle of a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention It is a schematic diagram.

1 and 2, a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention includes a case 20 and a measurement pad 30 and includes a camera 11 and a camera flash 13, The IT device 10 may be detachably attached to the apparatus.

The IT device 10 may be any device provided with a camera 11 and a camera flash 13 so long as it is capable of shooting and recording.

For example, the IT device 10 may be a mobile communication terminal such as a smart phone, a smart pad, a tablet PC, and a PDA. In addition, it is possible to analyze the measurement result based on the photographed image using the application installed in the IT device 10. [

The case 20 is provided with a support 25, a camera opening 21, and a camera flash opening 23.

In this case, a flexible plastic film such as an OHP film, PET, PS, PE, or the like may be used as the support 25, and a groove is provided so as to sandwich the paper. An adhesive or a double- And is attached to the inside of the case 20 in detail, it is preferable to install the camera at a maximum focal distance of the camera 11 capable of capturing an image of the measurement pad 30 in detail.

It is preferable that the camera opening 21 and the camera flash opening 23 are opened to correspond to the sizes of the camera 11 and the camera flash 13, respectively.

Therefore, the case 20 is configured so that the camera 11 of the IT device 10 and the camera flash 13 are exposed through the camera opening 21 and the camera flash opening 23 of the case 20, 10.

At this time, since the case 20 can be detached from the IT device 10, it can be mounted and used only when necessary.

Therefore, the case 20 may be formed in the same shape as a generally used smartphone case.

The case 20 is further provided with a lid 20a for blocking external light and is provided with a lid 20a and a fixing member 27 provided on the side of the case 20 as shown in Fig. The lid 20a may be fixed.

At this time, the fixing member 27 is a member for facilitating opening and closing of the lid 20a such as a magnet, a button or the like, and is made of a lid 20a and a case 20 Respectively.

In other words, the case 20 can form a dark room condition so that external light is blocked by the lid 20a and the fixing member 27, so that the influence of external light can be minimized.

The lid 20a of the case 20 may be provided with a reflector 26 for evenly dispersing the light emitted from the camera flash 13 into the case 20. [

More specifically, the reflective portion 26 may be a mirror-type reflective film, a mirror, a silver foil or the like used for an LCD display or the like, and may be attached to the inside of the lid 20a as shown in Fig. 1 by using an adhesive, .

A light splitting part 24 for adjusting the intensity of light emitted from the camera flash 13 may be attached to the camera flash opening 23 of the case 20.

More specifically, the light splitting unit 24 is an accessory for preventing over-irradiation of the camera flash 13 and thus a signal unevenness problem, and may be a light dispersion film used for an LCD display or the like, and may be a translucent cellophane film, May be used and attached to the opening 23 for the camera flash as shown in Fig. 1 by using an adhesive or double-sided tape or the like.

A rectangular prism portion 22 may be attached to the camera opening 21 of the case 20 to rotate the image of the measurement pad 30 at right angles to irradiate the camera 11 with the image.

More specifically, the right angle prism portion 22 may be made of glass or a transparent plastic material.

FIG. 3 is a perspective view illustrating a right angle prism unit in a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention. FIG.

3, only the light in the direction of the measurement pad 30 needs to be transmitted to the camera 11, so that it is preferable that the rectangular prism portion 22 is covered with black aluminum foil only on the faces 1, 2, and 3 .

In summary, the intensity of color development derived from the measurement pad 30 is measured by using the camera 11 of the IT device 10. In the process of measuring the photographed image, the brightness of the image is changed by the external light source, In order to minimize the interference of the light source, the case 20 allows the external light source to be blocked by the cover 20a and the fixing member 27 for an environment of a dark room condition.

In order to uniformly distribute the light emitted through the camera flash 13 to the inside of the case 20, the camera flash 13 of the IT device 10 is used to fill the light required for photographing under the dark room condition, (26), i.e., a mirror, is mounted on the inner ceiling of the ceiling (20).

At this time, the measurement pad 30 is positioned at the center of the case 20, and the light emitted through the camera flash 13 is uniformly spread inside the case 20 through the reflection part 26, The measurement section 32 reaches the lens of the camera 11 through the right prism section 22, whereby an image is captured.

The measurement pad 30 is supported by a support 25 of the case 20 as shown in FIG. 1, and includes an enzyme reaction material according to an object to be measured, and reacts according to a sample to be injected for measurement.

In detail, the measurement pad 30 may be formed in any form as long as it can cause an enzyme color reaction according to a sample to be injected for measurement. In an embodiment of the present invention, a process of manufacturing using paper and wax .

4 is a view illustrating a process of manufacturing a measurement pad in a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.

4, the measurement pad 30 prints a pattern on a paper sheet using a wax printer, and allows the wax to permeate into the paper sheet using an oven for one minute. At this time, the portion to which the wax is impregnated becomes hydrophobic, and the portion to which the wax is not impregnated becomes hydrophilic. That is, the liquid is allowed to flow only to a place where the wax is not printed using the point that flows only into the hydrophilic region.

FIG. 5 is a view showing the internal structure of a measurement pad in a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention, and FIG. 6 is a graph showing an internal structure of a measurement pad using an IT device according to an embodiment of the present invention, FIG. 3 is a view showing a process of reacting a sample injected into a measurement pad in a device. FIG.

As shown in FIG. 5, the measurement pad 30 adheres paper having two different patterns produced in FIG. 4 to each other with a double-sided tape 30c or the like. The upper layer 30a, which is an upper paper, The lower layer 30b, which is a lower layer paper, comprises an injection unit 31 and a measurement unit 32 including an enzyme reaction material mixed with one or more substances that cause color development by reacting with a sample. A channel 33 for moving the fluid to the measuring unit 32 is formed. In addition, the channel 33 may contain a surfactant in order to speed up the flow rate of the fluid.

At this time, a hemocyte separation film (not shown) may be further laminated on the injection unit 31 for injecting the blood and the serum sample in order to efficiently separate the blood cells and improve the signal sensitivity thereof. Or a filter made of paper or the like may be used.

6, when the blood is injected into the injection unit 31, the blood cells are filtered through the channel 33 of the lower layer 30b, and only the serum reaches the measurement unit 32, ) Reacts with an enzyme reaction substance contained in the enzyme to cause color development.

Therefore, the IT device 10 photographs the measurement pad 30 that has developed color through the camera 11 and the camera flash 13, analyzes the measurement result based on the photographed image, and performs rapid measurement and quantitative analysis .

Hereinafter, a method for measuring glucose concentration for diagnosing diabetes is described as an embodiment for diagnosing disease using a biological sample quantitative analysis apparatus using an IT device of the present invention.

Firstly, GOx, an enzyme used for the generation of hydrogen peroxide in the diagnosis of diabetes, and horseradish peroxidase (HRP), 4-aminoantipyrine and MAOS (N-Ethyl-N- the measurement part 32 is provided with an injection part 31 of the measurement pad 30 in which an enzyme reaction material according to an object to be measured is contained in the measurement part 32. In this case, The amount of the enzyme reaction substance included in the measurement part 32 was 10 mM 4-AAP, 1 mM MAOS, 1 mg / ml HRP, 10 mg / ml GOx. < / RTI >

The following formula (1) shows the principle of inducing a glucose color reaction through an enzyme reaction.

To describe Formula 1, glucose oxidase (GOx) oxidizes glucose to gluconic acid to produce hydrogen peroxide. This hydrogen peroxide is reduced by Horseradish peroxidase (HRP) and a coloring compound (4-aminoantipyrine ; 4-AAP, N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline, sodium salt monohydrate; MAOS).

At this time, as the concentration of glucose increases, the amount of hydrogen peroxide generated by GOx increases and the intensity of the blue color gradually increases. Therefore, the amount of glucose can be measured by analyzing the intensity of color development.

FIG. 7 is a diagram illustrating measurement results of a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention.

In order to measure the glucose in the blood, a serum containing 5 mM to 17 mM glucose was prepared and the change in the color intensity was measured using a camera (11) in 150 seconds for 10 seconds using 20 쨉 l.

7 (A) shows photographs of glucose concentration in serum obtained through a biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention, (B) shows (A) As can be seen from the graph shown in (B) of the result graph, it can be seen that as the concentration of glucose increases, the value also increases.

(C) is a graph showing the results of measurement using a general blood glucose meter for the glucose concentration of serum samples identical to those of (A) and (B), wherein a biological sample using an IT device according to an embodiment of the present invention And the result of measurement using the quantitative analysis apparatus is compared with the graph of (B) showing the result, the respective result values show almost a one-to-one correspondence. Therefore, the bio- It can be seen that the reliability of the apparatus is proved.

In addition, enzyme reactions for diagnosing diseases that can be additionally applied are shown in Table 1 below.

At this time, the analyte shown in Table 1 is a sample to be injected into the injection unit 31. The enzymes used for the analysis for generating hydrogen peroxide are mixed with HRP, 4-AAP and MAOS in the measuring unit 32 And materials which react with the sample injected into the injection unit 31 to cause color development. Table 1 below summarizes the chemical formulas for generating hydrogen peroxide.

Here, the principle of inducing a color reaction through enzyme reaction for each diagnostic field is as follows.

<Acetylcholine>

<Liver health examination item (ALT)>

<Liver Health Checkup Items (AST)>

<Total cholesterol>

<Metabolism and kidney related (Creatine)>

<Kidney and urine diagnosis (Creatinine)>

<Triglyceride>

Accordingly, the biological sample measuring apparatus according to one embodiment of the present invention can be used in a field where a reaction to induce color development is used, for example, a coloring reaction can be caused by reacting with a coloring compound (4-AAP, MAOS) It can be applied to various fields and diseases and has a wide range of use.

Further, the MAOS used for the color reaction may be a phenol-based material or other materials. Thus, examples of substances that can replace MAOS and their reactivity are as follows.

<ADOS>

<ADPS>

<ALPS>

<DAOS>

<HDAOS>

<MADB>

<TODB>

<TOOS>

<TOPS>

At this time, although the color of the color changes depending on the kind of the material, the measurement result is the same, so it is possible to apply any other one instead of MAOS.

As described above, the biological sample quantitative analysis apparatus using an IT device according to an embodiment of the present invention uses a camera and a camera flash provided in a smart phone as a light receiving unit and a light emitting unit to measure the degree of coloring derived from the concentration of a sample It is possible to perform accurate and quick analysis by analyzing the recorded results, and it can be used in conjunction with a smart phone, so that it is not necessary to have a separate device, and the result It is possible to maximize user convenience and satisfaction.

In addition, since an inspection sheet necessary for analysis of a biological sample, that is, a measurement pad is made of paper, the cost is low and it is operated by the capillary force inside the paper, so there is an advantage that a special external power source is unnecessary.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the invention as defined by the appended claims. will be. Accordingly, the true scope of the present invention should be determined by the following claims.

10: IT device 11: camera
13: camera flash 20: case
20a: lid 21: opening for camera
22: Right angle prism section 23: Camera flash opening
24: light distribution section 25:
26: reflection part 27: fixing member
30: Measurement pad 30a: Upper layer
30b: Lower layer 30c: Adhesive member
31: injection part 32: measuring part
33: channel

Claims (7)

A case detachably attached to an IT device provided with a camera and a camera flash and including a support, an opening for a camera, an opening for a camera flash, a cover for blocking external light, and a fixing member for fixing the cover; And
And a measurement pad supported by a support of the case and containing an enzyme reaction material according to an object to be measured and reacting according to a sample to be injected for the measurement,
In this case,
Wherein a right prism portion for rotating the image of the measurement pad at a right angle and irradiating the camera is attached to the opening for the camera and a light splitting portion for adjusting the intensity of light emitted from the camera splash is attached to the camera flash opening And a reflection part for uniformly dispersing the light emitted from the camera flash into the case is attached to the cover,
The support includes:
Wherein the measurement pad is provided with a groove through which the measurement pad can be inserted, and is installed at a maximum focusable distance of the camera. The method according to claim 1,
The IT device includes:
The camera and the camera flash are mounted on the case so that the camera and the camera flash are exposed through the camera opening and the camera flash opening, and the camera and the camera flash are used to photograph the measurement pad, A biological sample quantitative analysis device using an IT device for analyzing the result of the analysis. The method according to claim 1,
Wherein the measurement pad comprises:
And a measurement unit including the injection unit to inject the sample and the enzyme reaction material in which one or more substances that react with the sample and cause color development are mixed. delete delete delete The method according to claim 1,
The enzyme reaction material may contain,
HRP (Horseradish peroxidase) and 4-AAP (4-aminoantipyrine)
And further comprises at least one of GOx (Glucose oxidase), AChE (Acetylcholine), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (CE), creatine (CT), creatinine (CA), and triglyceride ,
MAOS (N-Ethyl-N- (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline, sodium salt monohydrate), ADOS , sodium salt, dihydrate), ADPS (N-Ethyl-N- (3-sulfopropyl) -3-methoxyaniline, sodium salt, monohydrate) DAOS (N- (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline, sodium salt), HDAOS (N- ), MADB (N, N-bis (4-sulfobutyl) -3,5-dimethylaniline, disodium salt), TODB (N-ethyl-N- (3-sulfopropyl) -3-methylaniline, sodium salt, monohydrate) and phenol A biological sample quantitative analysis apparatus using an IT device including one further.

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