201211262 六、發明說明： 【發明所屬之技術領域】 [0001] 本發明有關於一種二階段培養藻類生質柴油生產技 術，其藻類生成的步驟可分成第一階段培養、第二階段 培養，主要將藻體生成與油脂生成的方式分成兩階段， 以有效控制藻類脂質的生長者。 【先前技術】 [0002] 按，習用藻類培養方式，依據藻類的種類，不同的 藻類的脂質含量有明顯的差異，且生長環境的不同，同 藻屬間的脂質含量也會有很大的不同，影響藻類脂質含 量的因素，包括物理因子與化學因子，在化學因子方面 ，首先是營養鹽，改變營養鹽的成分可能會影響藻細胞 的脂質含量，有研究結果指出某種綠藻細胞處在低氮營 養的環境下，其脂質含量比控制組高，亦有一種矽藻在 矽、磷等營養鹽的供給受到限制時，其脂質含量會高於 未受限制的，但在大規模生產時，若要使單位培植面積 内達到最佳化就必須要同時達到藻類生長速度快，及藻 類内油含量高兩個目標，但就以目前藻類培養技術，這 兩個目標剛好相互矛盾者。 【發明内容】 [0003] 其上述習用的技術，改變營養鹽的成分可能會影響 藻體細胞的脂質含量，但要藻體同時達到生長速度快， 及油脂含量高的兩個目標，以目前藻體培養技術，此兩 個目標剛好相互抵觸者。 本發明主要目的在於提供一種二階段培養藻類生質 099130633 表單編號A0101 第4頁/共10頁 0992053783-0 201211262 柴油生產技術，將藻類的培養分成藻體的生長與脂質的 生成，使單位面積的藻類脂質產量能夠提高者。 為達前揭之目的，本發明二階段培養藻類生質柴油 生產技術，利用培養基的不同使藻類的生長速度與脂質 的生成分成兩階段，使藻體高濃度脂質的生成可更為顯 著，該二階段培養藻類生質柴油生產技術包含有第一階 段培養與第二階段培養所構成，該第一階段培養，係由 藻體、光反應器A、培養基A、成長最佳密度、分離、 θ 濃縮藻體所組成，其藻體，係為可生成脂質的單細胞微 藻，其光反應器A，係為培養藻體生成的裝置，其培養 基A，係供給藻體生成所需的營養鹽，其成長最佳密度 ，係為藻體於單位面積内的最大生長量，其分離，係將 外形生長完成的藻體進行固液兩相的分離動作，其濃縮 藻體，係經分離動作所分離之藻體；該第二階段培養， Ο 係由光反應器B、培養基B、高濃度脂質生成、萃取油 脂、成品所組成，其光反應器B，係為培養濃縮藻體脂 質生成的裝置，其培養基B，係供給濃縮藻體的脂質生 成所需的營養鹽，其高濃度脂質生成，係由培養基B促 進濃縮藻體内部的油脂生成，其萃取油脂，係將經由完 成培養脂質的濃縮藻體進行油脂萃取動作；該成品，係 為濃縮藻體經萃取後所得之生質油脂。 本發明將藻體脂質的生成分成二階段培養，第一階 段培養，先將藻體的單位面積的產量提高，再進行第二 階段培養，使藻體内的脂質生成，將生長與脂質的生成 分開，可避免藻體快速生長並同時生成高濃度脂質的技 術問題者。 099130633 表單編號A0101 第5頁/共10頁 0992053783-0 201211262 【實施方式】 [0004] 本發明之二階段培養藻類生質柴油生產技術，〔請 參閱第-圖〕其包括第一階段培養⑴與第二階段培 養（1 )所構成；其中： 第一階段培養（1 )，係由藻體（丄丄）、光反應 器A ( 1 2)、培養基A ( 1 3 )、成長最佳密度（丄 4)刀離（15)、》辰縮薄體（16)所組成，其藻 體（1 1)，係為可生成脂質的單細胞微藻，經由第一 階段生長過遽得濃縮藻體（16)，其光反應器 3)，係為培養薄體（1 1 )生成的裝置，其培養基A (14)，係供給薄體（11)的脂質生成所需的營養 鹽，含氮、峨量高的培養基A(14)，可促進藻 1 1 )外形的生長，讓藻體（！ i )生長週期可大為縮 減，其成長最佳密度（1 5)，係為藻體（丄i )於單 位面積内的最大生長量’其分離（16)，係將外形生 長完成的藻體（1 1)進行固液雨相的分離動作，以取 得經分離後的濃縮藻體(i 6〇 ，再進入第二階培養（2)的流程; 第二階段培養（2)，係由光反應器B(21)、 培養基B (2 2)、高濃度脂質生成（2 3 )、萃取油 脂（2 4)、成品（2 5)所組成，其光反應器B (2 1)，係為培養濃縮藻體脂質生成的裝置其 培養基B ’係供給濃祕體（i 6 )的脂質生成所需的 營養鹽，含氮、磷量低的培養基B ( 2 1 ),可促進濃 縮藻體（1 6 )内脂質的生成，其高濃度脂f生成（2 3)，係由培養基B(22)促進濃縮藻體（16)内 099130633 表單編號A0101 第6頁/共10頁 0992053783-0 201211262 部的油脂生成，其萃取油脂（24)，係將經由完成培 養脂質的濃縮藻體（1 Θ)進行油脂萃取動作，其成品 (2 5)，係為濃縮藻體（16)經萃取後所得之生質 油脂。 使用實施二階段培養藻類生質柴油生產技術時，〔 請參閱第一圖〕將藻體（工工）月旨質的生成分成二階段 培養· 第一階段培養（1 ):201211262 VI. Description of the Invention: [Technical Field of the Invention] [0001] The present invention relates to a two-stage cultivation technology for algae biodiesel production, wherein the steps of algae production can be divided into a first stage culture and a second stage culture, mainly The way in which algae formation and oil production occurs is divided into two stages to effectively control the growth of algal lipids. [Prior Art] [0002] According to the conventional algae cultivation method, depending on the type of algae, the lipid content of different algae is significantly different, and the growth environment is different, and the lipid content between the algae is also very different. Factors affecting the lipid content of algae, including physical factors and chemical factors. In terms of chemical factors, firstly, nutrients, changing the composition of nutrients may affect the lipid content of algae cells. Some research results indicate that certain green algae cells are present. In the low-nitrogen environment, the lipid content is higher than that of the control group. There is also a case where the supply of nutrients such as earthworms and phosphorus is limited, and the lipid content is higher than that of unrestricted, but in large-scale production. In order to optimize the cultivation area of the unit, it is necessary to simultaneously achieve the two goals of rapid growth of algae and high oil content in algae, but with the current algae cultivation technology, these two goals are just contradictory. SUMMARY OF THE INVENTION [0003] The above-mentioned conventional techniques, changes in the composition of nutrients may affect the lipid content of algal cells, but the algae simultaneously achieve two growth goals, and high oil content, the current algae Body culture technology, these two goals are just against each other. The main object of the present invention is to provide a two-stage culture algae biomass 099130633 Form No. A0101 Page 4 / 10 pages 0992053783-0 201211262 Diesel production technology, the algae cultivation is divided into algae growth and lipid production, so that the unit area Algal lipid production can be improved. For the purpose of the prior disclosure, the two-stage cultivation technology of algae biodiesel in the present invention utilizes different culture media to divide the growth rate of algae and the production of lipid into two stages, so that the formation of high concentration lipids of algae can be more remarkable. The two-stage culture algae biodiesel production technology comprises a first stage culture and a second stage culture, the first stage culture is performed by algae, photoreactor A, medium A, growth optimal density, separation, θ The concentrated algae is composed of a single cell microalgae capable of producing lipids, and the photoreactor A is a device for cultivating the formation of algae, and the medium A is a nutrient salt required for the production of algae. The optimal growth density is the maximum growth of algae in a unit area. The separation is performed by separating the solid-liquid two-phase separation of the algae body with the shape growth, and the concentrated algae body is separated. The isolated algae body; the second stage culture, the lanthanum is composed of photoreactor B, medium B, high-concentration lipid formation, oil extraction, and finished product, and photoreactor B is cultured and concentrated. The apparatus for producing body lipids, wherein the medium B is a nutrient salt required for the production of lipids in the concentrated algal body, and the high-concentration lipid is formed by the medium B to promote the formation of oil and fat inside the concentrated algae body, and the oil is extracted by the medium B. The concentrated algae body for culturing the lipid is subjected to a fat extraction operation; the finished product is a raw fat obtained by extracting the concentrated algae body. The invention divides the production of algal lipid into two-stage culture, the first stage culture, firstly increases the yield per unit area of the algae, and then carries out the second stage culture, the lipid formation in the algae, the growth and the formation of lipids. Separation can avoid the technical problems of rapid growth of algae and simultaneous production of high concentrations of lipids. 099130633 Form No. A0101 Page 5 / Total 10 Page 0992053783-0 201211262 [Embodiment] [0004] The two-stage cultivation technology of algae biodiesel in the present invention, [see Fig. 1] includes the first stage culture (1) and The second stage culture (1) is composed; wherein: the first stage culture (1) is composed of algae (丄丄), photoreactor A (1 2), medium A (13), and optimal growth density (丄 4) The knife is separated from the (15) and the thin body (16), and the algae (1 1) is a single-celled microalgae capable of producing lipids, and the concentrated algae are grown through the first stage. (16) The photoreactor 3) is a device for cultivating a thin body (1 1 ), and the medium A (14) is a nutrient salt required for the production of lipids of the thin body (11), containing nitrogen, Medium A (14) with high amount of sputum can promote the growth of algae 1 1 ), so that the growth cycle of algae (! i ) can be greatly reduced, and the optimal density of growth (1 5) is algae (丄i) The maximum growth amount per unit area's separation (16) is the separation of the solid-liquid rain phase by the algae body (1 1) whose shape growth is completed. In order to obtain the separated concentrated algae (i 6〇, then enter the second-stage culture (2) process; the second stage culture (2), from the photoreactor B (21), medium B (2 2 ), high-concentration lipid production (2 3 ), extraction of oil (2 4), and finished product (25), and photoreactor B (2 1), which is a device for cultivating concentrated algal lipids, and its medium B ' It is a nutrient salt required for the production of lipids from the secret body (i 6 ), and a medium B ( 2 1 ) containing a low nitrogen and phosphorus amount, which promotes the formation of lipids in the concentrated algae (16), and its high concentration of lipid f generation (2 3), promoted by concentrated medium algae (16) by medium B (22) 099130633 Form No. A0101 Page 6 / Total 10 pages 0992053783-0 201211262 Oil production, extraction of oil (24), The concentrated algae (1 Θ) that has been subjected to the culture of the lipid is subjected to a fat extraction operation, and the finished product (25) is a raw oil obtained by extracting the concentrated algae (16). The two-stage cultivation of the algal biomass is carried out. In the diesel production technology, [see the first figure], the formation of the algae (worker) monthly quality is divided into two stages. Segment culture (1):
首先選擇所欲培養含油脂量高的藻體（1 1 )，並 進行第一階段的藻體（i i )培養，將所選定培養的藻 體（1 1 )置入光反應器A:( 1:2 )肩，藉由設置在光 反應器A ( 1 2 )内的培養基a ( 1: 3 )進杆藻體（1 1 )的培養’經數小時後’其藻體（11 )可達到成長 最佳密度（14)，其成長最佳密度（14)係為單位 面積的最大生長量，此時，將藻體（)進行分離（First, select the algae (1 1 ) to be cultured with high oil content, and carry out the first stage of algae (ii) culture, and place the selected cultured algae (1 1 ) into photoreactor A: (1) : 2 ) Shoulder, by the culture medium a ( 1: 3 ) set in the photoreactor A ( 1 2 ) into the culture of the algae (1 1 ) 'after a few hours' its algae (11) can be reached The optimal density for growth (14), the optimal density for growth (14) is the maximum growth per unit area, at which point the algae () is separated (
1 5 )的動作，其分離（1 5 ),係利用固液兩相過濾的 方式，經分離（1 5)過濾後可得濃縮藻體（1 6)， 以完成第一階段培養（1 )並進入第二階段培養（2 ) 9 第二階段培養（2): 進行第二階段培養（2)時’將經過濾濃縮藻體（ 1 6)置入光反應器B (2 1 )内，藉由設置在光反應 器B ( 2 1 )内的培養基B (2 2)進行濃縮藻體（1 6)内的高濃度脂質生成（23)，經數小時後，其濃 縮藻體（1 6)内的高濃度脂質含量達最高，此時，將 含有高濃度脂質的濃縮藻體（16)進行萃取油脂（2 099130633 表單編號A0101 第7頁/共10頁 〇992053783-0 201211262 4)，經萃取油脂（2 4)後可得萃取脂質之成品（2 5 )者。 其優點在於，利用培養基A ( 1 2 )與培養基B ( 22)含氮、磷量的不同使藻體（1 1)的生長與脂質 的生成分成兩階段，先將藻體（1 1 )的單位面積的產 量提高，再進行藻體（11)内的脂質生成，將生長與 脂質生成的步驟分開，可避免藻體（1 1 )快速生長並 同時生成高濃度脂質的技術問題者。 綜上所述，當知本發明具有新穎性，且本發明未見 之於任何刊物，當符合專利法第2 1、2 2條之規定。 唯以上所述者，僅為本發明之一較佳實施例而已， 當不能以之限定本發明之範圍。即大凡依本發明申請專 利範圍所作之均等變化與修飾，皆應仍屬本發明專利涵 蓋之範圍内。 【圖式簡單說明】 [0005] 第一圖：本發明之培養流程示意圖 [0006] 【主要元件符號說明】 1 第一階段培養 12 光反應器A 14 成長最佳密度 16 濃縮藻體 2 1 光反應器B 23 向濃度脂質生成 2 5 成品 22 2 2 4 藻體 培養基A 分離 第二階段培養 培養基B 萃取油脂 099130633 表單編號A0101 第8頁/共10頁 0992053783-0The action of 1 5 ) is separated (1 5 ) by means of solid-liquid two-phase filtration, and after separation (15) filtration, concentrated algae (1 6) can be obtained to complete the first stage culture (1). And enter the second stage of cultivation (2) 9 the second stage of cultivation (2): When the second stage of cultivation (2) is carried out, 'the filtered concentrated algal body (16) is placed in the photoreactor B (2 1 ), The high concentration of lipids (23) in the concentrated algae (16) is carried out by the medium B (2 2) provided in the photoreactor B (21), and after several hours, the algae are concentrated (1 6 The high concentration of lipids in the high concentration is high. At this time, the concentrated algae (16) containing high concentration of lipids are extracted and extracted (2 099130633 Form No. A0101 Page 7 / 10 pages 〇992053783-0 201211262 4), After extracting the oil (2 4), the finished product (25) can be obtained. The advantage is that the difference between the nitrogen content and the phosphorus content of the medium A (1 2 ) and the medium B (22) is used to divide the growth of the algae (11) and the formation of lipid into two stages, firstly the algae (1 1 ) The yield per unit area is increased, and the formation of lipids in the algal body (11) is carried out, and the steps of growth and lipid formation are separated, and the technical problem of rapid growth of algae (1 1 ) and simultaneous formation of high-concentration lipids can be avoided. In summary, it is known that the present invention is novel, and the present invention is not found in any publication, and is in compliance with the provisions of Articles 21 and 22 of the Patent Law. The above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited thereto. That is, the equivalent changes and modifications made by the applicant in accordance with the scope of the patent application of the present invention are still within the scope of the invention. BRIEF DESCRIPTION OF THE DRAWINGS [0005] First: Schematic diagram of the culture process of the present invention [0006] [Explanation of main component symbols] 1 First stage culture 12 Photoreactor A 14 Growth optimal density 16 Concentrated algae 2 1 Light Reactor B 23 to concentration of lipids 2 5 Finished product 22 2 2 4 Algae medium A Separation of second stage culture medium B Extraction of oil 099130633 Form No. A0101 Page 8 of 10 0992053783-0