201043692 六、發明說明： 【發明所屬之技術領域】 本發明係錢於-纖錄轉設備，_是指—種可滅菌之 生物培養設備及其培養方法。 【先前技術】 Ο ❹ 近年來，微生物的顧大·1¾善人躺生活品f，微生物的使用範圍 廣泛’-般多糊於_、害蟲、雜草㈣辆發作物，或是由微生物的 有效成分驗配謂製成產品，-般由自然界分離所得，惟也可再經人工 品系改良，如人為誘變、汰選或遺傳細改造。峨生物製作的產品多半 無毒性，而且在環境中能被生物所分解。微生物麵眾多，包含細菌、真 菌、藻類、原生動物或病毒。其作關制可經由#素的生產（如蘇力菌，、 勤齡、侵入寄生（如白彊菌，細睛吻）、病原性（如桿 狀病毒）和競爭（如病毒用於植物之交又保護）。 干 微生物可針對特定的害蟲作有__，對人或其他動植⑽毒性， =因其具生物可分解性，所料會_污_境，長久絲即已商業化大 量生產用於蟲害的防治、然而-般微生物培養不易，必須以特定的裳 設備才能培概生物，域定的裝置或設備造_高，成本料大量使用 所能負擔。軸微生物培養的研究於文獻上之記载頗多，技術上 =多為實驗室的研究成果，而且卫業化培養的成果報告因屬機密性質，、外 =交不易得知内容。而實驗室的微生物酸酵槽，再配備附屬之域 蒸汽麵、冰水機、桶槽、管路等，視品牌規格，需花費—百至r 使用者根本負擔不起這麼魔大的費用。另外，有些使用者、農家或 養i質桶自行生產農用或其他用途的微生物，但因無培 土質預先顧的程序，通人之空氣亦無除 溫控設備，所轉養過程被污染料自知，培養效果- 製微生物的培養方式，幾丰益不葙古缸如山 至於小型自 ⑼4手無獻報告。因此本發明提供一種可滅菌 之簡便试生物培養設備，其結顧單，成本低廉，操作料，且可避免雜 201043692 菌污染’以讓使用者以低成本的方式自行培輕生物而使用。 【發明内容】 本發明之目的之一， 培養方法，其結構簡單， 用。 在於提供—種可滅菌之簡便微生物培養設備及其 以讓使用者自行以低成本的方式培養微生物而使 拉美u之目的之一在於提供—種可滅菌之簡便微生物培養設備及其 二二:其係藉由設置於一培養槽之—空氣渡器以將細_滅，並藉 通氣管通人氣體’使氣體經由培養槽之一排氣口排出，如此可使培 養槽内之工氣不斷排出’以避免細H由外界進人培養槽内*污染培養之 生物。 本f明之可滅狀簡便微生物培養設備，包含—培養U掉裝置、 通氣管與-加錄置；培频，有—職σ ;麟裝置穿設於培養槽之 側邊；通氣管穿設於培養槽之側邊，通氣管通入一氣體，氣體經由排氣口 排出；空氣過濾器設於培養槽内並與通氣管相接設；加熱裝置設於培養槽 之底端；冷卻裝置設於培養槽内。本發明不但結構簡單，製作成本低廉， 以讓使用者以低成本的方式自行培養微生物而使用，且藉由設置於培養槽 内的空氣過濾器以將細菌消滅，並使通氣管通入氣體再經由排氣口排出的 方式，可使培養槽内之空氣不斷排出，以避免細菌由外界進入培養槽内而 污染培養之微生物。 本發明之可滅菌之簡便微生物培養設備其培養方法，包括：（_)培養 基煮沸滅菌：將培養基置於一培養槽内，並加熱煮沸；（二）導入空氣： 在培養基煮沸滅菌結束前導入空氣於煮沸之培養基中，該空氣係經—設於 該培養槽内之空氣過遽器；（二）冷卻培養基；（四）無雜菌接種：將種菌 液於無雜菌狀態下倒入培養槽中完成接種；（五）維持「正壓」於培養操作 期間。 201043692 【實施方式】 兹為使貴審查委員對本發明之結構、方法特徵及所達成之功效有更 進-f之瞭解與認識，難啸佳之實補魏合詳蚊綱，說明如後： 明參閱第-圖與第二圖’其係為本發明較佳實闕之可鋪之簡便微 生物培養設備之舰W與上視圖；本發明之可滅菌之簡倾生物培養設 備，包含-培養槽10、-授拌裝置2〇、一通氣管3〇與一加熱裝置4〇;培 養槽10上方具有-排氣口 12;攪拌裝置2〇穿設於培養槽1〇之側邊·，通氣 f 30穿設於培養槽10之側邊，通氣管3G藉由-空氣壓縮機通人-氣體， 氣體可為工H體經由排氣口 12排出；加熱裝置4Q設於培養槽1〇之底201043692 VI. Description of the Invention: [Technical Field to Be Invented by the Invention] The present invention relates to a money-to-fibre recording device, and _ refers to a sterilizable biological culture device and a culture method thereof. [Prior Art] Ο ❹ In recent years, the micro-organisms of the Gu Da·13⁄4 good people have lived in a wide range of products, and the use of microorganisms has been extensively---------------------------------------------------------- The test is made into a product, which is generally separated from the natural world, but can also be modified by artificial lines, such as artificial mutagenesis, selection or genetic modification. Most of the products made by cockroaches are non-toxic and can be broken down by organisms in the environment. Microorganisms are numerous and contain bacteria, fungi, algae, protozoa or viruses. Its production system can be produced through the production of #素素 (such as Suri, bacteria, invasive parasites (such as white strong bacteria, fine kiss), pathogenic (such as baculovirus) and competition (such as viruses used in plants) Crossing and protection) Dry microbes can be __ for specific pests, toxic to humans or other animals (10), = due to their biodegradability, expected to be _ _ _ _, long-term silk has been commercialized Production is used for the control of pests, however, microbial culture is not easy. It is necessary to use specific equipment to cultivate organisms. The equipment or equipment used in the field is high and the cost can be used in large quantities. The research on shaft microbial culture is in the literature. There are quite a lot of records on it. Technically, it is mostly the research results of the laboratory, and the report on the results of the industrialization training is classified as confidential, and the foreign exchange is not easy to know. The laboratory microbial acid fermentation tank, Equipped with a subsidiary domain steam surface, ice water machine, tank, pipeline, etc., depending on the brand specifications, it costs - hundred to r users can not afford such a huge cost. In addition, some users, farmers or raise i The barrel itself produces agricultural or other Microorganisms for use, but because of the procedures without pre-existing soil quality, there is no temperature control equipment for the air passing through, and the breeding process is known by the pollutants. The culture effect - the cultivation method of microbes, The cylinder is as small as the small (9) 4 hands without report. Therefore, the present invention provides a simple and sterilizable biological test equipment, which can be used for the convenience of the single, low cost, operating materials, and can avoid the pollution of the 201043692 bacteria to make the user low. The invention relates to a method for cultivating a light organism by itself. The invention has the advantages of simple structure and a culturing method, and provides a simple microbial culture device capable of sterilizing and so as to let the user lower himself. One of the purposes of cultivating microorganisms in a cost-effective manner is to provide a simple microbial culture apparatus that can be sterilized and two or two thereof: it is cooled by an air trap disposed in a culture tank, and By using a ventilator to pass the gas, the gas is discharged through one of the vents of the culture tank, so that the work gas in the culture tank can be continuously discharged to avoid the fine H from being cultivated by the outside world. The organism in the tank* is contaminated and cultivated. The simple microbial culture equipment of the present invention includes: the culture U-drop device, the vent pipe and the -recording; the frequency, the - σ; the lining device is placed in the culture tank a side pipe; a vent pipe is disposed on a side of the culture tank, a gas is introduced into the vent pipe, and the gas is discharged through the exhaust port; the air filter is disposed in the culture tank and connected to the vent pipe; the heating device is disposed in the culture tank The bottom end of the cooling device is disposed in the culture tank. The invention has the advantages of simple structure and low production cost, so that the user can self-cultivate the microorganisms in a low-cost manner, and the air filter is disposed in the culture tank. The bacteria are eliminated, and the vent pipe is introduced into the gas and discharged through the exhaust port, so that the air in the culture tank can be continuously discharged to prevent the bacteria from entering the culture tank from the outside to contaminate the cultured microorganisms. The method for cultivating the simple microbial culture equipment comprises: (_) culture medium boiling sterilization: placing the culture medium in a culture tank and heating and boiling; (2) introducing air: in the medium Before the end of boiling sterilization, air is introduced into the boiled medium, and the air is passed through an air filter set in the culture tank; (2) cooling medium; (4) no bacteria inoculation: the inoculum is in the absence of bacteria Pour into the culture tank to complete the inoculation; (5) Maintain "positive pressure" during the cultivation operation. 201043692 [Embodiment] In order to enable the reviewing committee to have a better understanding and understanding of the structure, method features and effects achieved by the present invention, it is necessary to understand the understanding and understanding of the results of the invention. Figure 2 and the second figure 'is a preferred embodiment of the present invention, a simple microbial culture equipment ship W and a top view; the sterilizable simple tilt biological culture apparatus of the present invention, comprising - culture tank 10, - Mixing device 2〇, a vent pipe 3〇 and a heating device 4〇; the top of the culture tank 10 has an exhaust port 12; the stirring device 2 is disposed on the side of the culture tank 1〇, and the ventilation f 30 is placed in the culture On the side of the tank 10, the vent pipe 3G is passed through a gas-gas compressor, and the gas can be discharged through the exhaust port 12; the heating device 4Q is disposed at the bottom of the culture tank.
Q 端。 本發明係时有蘇力菌之微生物的製作為範例作說明。*製作微生物 之培養槽H)之雜可_鐵。树敗裝置2Q包含—勝馬達22、 擾拌軸24與-餅葉片26 ’授拌馬達22設於培養槽之外側丨谢棒 24穿叹於培養槽1〇之側邊並與授摔馬達22相接設；授摔葉片％設於授抖 軸24並位於培養槽1G内。另外，勝馬達四可再接設一變流器以調整賴 拌馬達22轉速’而本發明將娜馬達22之轉數調整為綱_左右。 本發明製作微生物之騎基質可職纽絲適當地調配並調璧 pH將培養基質灌人谷積約為1〇〇公升之培養槽至約7成滿。培養基賀 在授拌通氣培養的過程巾會起泡’故預先酌量加人消細。接著以瓦斯權 加熱裝置40將培養基質煮彿。本發明之加熱裝置4〇可為一瓦斯爐（或其付 力:熱器\而於培養槽1〇之—側設有溫度計60。在瓦斯爐火將培養基質邊 部後’接著將瓦火轉小’並且維持培養基f於彿騰狀態 ，使培養基Ϊ ';】、時。而本發曰月之培養槽W之排氣口蓋14可藉槽内壓力自七 開=本發収可於排氣置氣孔142，並將排氣口蓋Μ蓋緊，相 f槽10的礼體由乳孔142不斷排出，因此可使培養槽1〇保持半開放法 態’排出過多蒸汽或空氣。 本發明之通氣㈣財—人風σ 32與出風σ 34，人風σ 32位於培4 槽10之外出風口 34位於培養槽1〇之内。入風口 32之位置高於出風[ 5 201043692 34之^置。如此可使乾淨的氣體經由沸騰的培養基質加熱滅菌後，在從培 養基質内溢出於排氣口 12外，如此可避免細菌由排氣口 12内進入培養^ 質，入風口 32之位置通常也會高於培養基質之最高液位，以避免培養基質 從入風口 32流出。 本發明更於培養槽10内設置一空氣過濾器36，空氣過濾器36與通氣 管30相接設，藉以將輸入於培養基質之氣體藉由空氣過遽器％之一渡膜 過濾’以濾除空氣中之雜質或細S，以產生乾淨的氣體。濾、膜材質可為疏 水性之聚四氟乙稀（P·)或是其他材質，滤膜之孔隙約為Q.胸· 26 ^ 米（如較佳為G. 22齡），柯視實際S求而更換;f同孔社雜。培養基 質加熱滅®時’空氣過_ 36及其下游空氣管路—併受熱關。在培養基 質彿騰滅g完成前十分鐘’開啟空氣壓縮機灌人經除濕的空氣，流量約 =〜1〇〇公升/分鐘，將空氣過濾!！ 36下游培養基f液面之上的管路内可 月b仍帶雜_找’吹進》轉的培養基質中充分_。空氣流經空氣過據 益36及其下游管路後再由出風口 34 S{fc無菌的空氣泡，無菌空氣再由培 養槽10之排氣口蓋14的氣孔142或是排氣口 12排出。培養槽1〇自此時 起至培養結束’-直維持灌人除g空氣，及無菌的空氣自氣孔142或排氣 口 12排出’以「正壓」避免有雜_空氣進人培養槽1() β，此時起一直 維持灌入除g空氣，亦可避免後續培養基質冷卻時培養基質倒吸入空氣過 濾器36而損壞過濾器。 本發明更包含多孔之一氣泡石38，氣泡石38設於培養槽1〇内並與通 ，管30相接設’氣泡石38具有多孔隙，可使乾淨氣體打入培養基質時， 氣泡變的較細小且平均。 本發明於培養槽1〇内設置—冷卻裝置5〇，冷卻装置5〇更包含一冷卻 g 52 ’冷卻管52為一螺管，冷卻I； 52具有一輸入端522與一輸出端524， 輸入端522與輸出端524皆位於培養槽1〇外。輸入端522之位置低於輸出 端524之位置。在培養基質滅菌完成後，將冷卻管52之輸入端522通入冷 部液體，例如水，並自冷卻管52之輸出端524流出。藉由冷卻管52以使 培養基質降溢至30t ’將培養槽1()之排氣口蓋14打開一小縫，以維持無 201043692 菌二氣由縫中不斷排^在上吹的無菌氣流中，將_種菌液倒人培養槽ι〇 中以70成接種。蓋好培養槽10之排氣口蓋14，啟動授摔裝置2〇，並使授 拌馬達22維持約3〇〇rpm。 »培養槽10置於室峨涼處，培養触巾注意、將培養基質維持溫度 在蘇力菌最佳生長,皿度3〇 士 2c ’必要時開啟加熱裝置4〇或冷卻裝置5〇 調整溫度。培養過程中無其他的控制，如pH、親、溶解氧氣的控制。培 養過程約三日内可完成。完成後自產品流出口 16收集之培養液可立即使 用，或者將培養液冷藏，日後再用。 基於上述酬，本發明之培養方法包括：Q end. The present invention is illustrative of the production of microorganisms having S. cerevisiae. * Produce microbial culture tank H) Miscellaneous _ iron. The tree defeating device 2Q includes a wins motor 22, a spoiler shaft 24, and a cake blade 26. The mixing motor 22 is disposed outside the culture tank. The thank-you bar 24 is sighed on the side of the culture tank 1〇 and is coupled to the drop motor 22 The connection is provided; the drop blade % is set on the shaker shaft 24 and is located in the culture tank 1G. In addition, the win motor 4 can be further connected with a current transformer to adjust the rotational speed of the hybrid motor 22, and the present invention adjusts the number of revolutions of the nanomotor 22 to about _. The preparation of the microorganisms of the microorganisms of the substrate can be properly formulated and adjusted to pH. The culture medium is filled with a grain storage tank of about 1 liter to about 7 liters. The medium will be foamed in the process of the aeration and aeration process, so the amount is reduced in advance. The culture medium is then boiled with a gas weight heating device 40. The heating device 4 of the present invention may be a gas furnace (or its force: a heater\ and a thermometer 60 on the side of the culture tank). After the gas stove fires the material edge, the tile fire is followed. Turn to small 'and maintain the medium f in the state of Fo Teng, so that the medium Ϊ ';], and the venting cover 14 of the culture tank of the hairpin can be borrowed from the pressure in the tank from the seven open = the hair can be discharged The air is provided in the air hole 142, and the exhaust port cover is tightly closed, and the body of the phase f slot 10 is continuously discharged from the milk hole 142, so that the culture tank 1〇 can be kept in a semi-open state to discharge excessive steam or air. Ventilation (four) wealth - human wind σ 32 and wind σ 34, the human wind σ 32 is located outside the cultivating tank 4 and the outlet 34 is located within 1 培养 of the culture tank. The position of the air inlet 32 is higher than that of the wind [ 5 201043692 34 In this way, the clean gas can be heated and sterilized through the boiling culture medium, and then overflows from the culture medium to the outside of the exhaust port 12, thereby preventing the bacteria from entering the culture through the exhaust port 12, and the position of the air inlet 32. It is usually also higher than the highest level of the culture medium to avoid the culture medium flowing out of the air inlet 32. In the present invention, an air filter 36 is disposed in the culture tank 10, and the air filter 36 is connected to the vent pipe 30, so that the gas input into the culture medium is filtered by one of the air filter devices. Filter out impurities or fine S in the air to produce a clean gas. The filter material can be hydrophobic polytetrafluoroethylene (P·) or other materials, and the pores of the filter are about Q. Chest 26 ^ m (e.g., preferably G. 22 years old), the replacement of the actual S is required; f is the same as the hole. When the culture medium is heated, the air is _ 36 and its downstream air line - and is closed by heat. The culture medium is completed ten minutes before the completion of the air compressor to open the air compressor to dehumidify the air, the flow rate is about ~ ~ 1 〇〇 liter / minute, the air will be filtered!! 36 downstream medium f in the pipeline above the liquid level The monthly b is still mixed with _ looking for 'blowing in' the medium is sufficient. The air flows through the air through the benefit 36 and its downstream pipeline and then from the air outlet 34 S{fc sterile air bubble, sterile air The vent 142 of the vent cap 14 of the culture tank 10 or the vent 12 is discharged. The culture tank 1 〇 from this time to the culture The bundle '- directly maintains the man's air except for g, and the aseptic air is discharged from the air hole 142 or the exhaust port 12' to "positive pressure" to avoid miscellaneous air entering the culture tank 1 () β, and the irrigation is maintained at this time. The addition of g air can also prevent the culture medium from being sucked into the air filter 36 to damage the filter when the culture medium is cooled. The present invention further comprises a porous bubble stone 38, which is disposed in the culture tank 1 When the tube 30 is connected, the bubble stone 38 has a multi-porosity, and when the clean gas is driven into the culture medium, the bubbles become finer and evener. The present invention is provided in the culture tank 1—-cooling device 5〇, the cooling device 5 The 包含 further includes a cooling g 52 'cooling tube 52 is a solenoid, cooling I; 52 has an input end 522 and an output end 524, the input end 522 and the output end 524 are located outside the culture tank 1 . The position of input 522 is lower than the position of output 524. After the sterilization of the culture substrate is completed, the input end 522 of the cooling tube 52 is passed to a cold liquid, such as water, and flows out of the output end 524 of the cooling tube 52. The vent cap 14 of the culture tank 1 () is opened by a cooling pipe 52 to reduce the culture medium to 30 t', so as to maintain the absence of 201043692 bacteria in the aseptic airflow continuously discharged from the slit. The phage liquid was poured into a culture tank and inoculated with 70%. The vent cover 14 of the culture tank 10 is covered, the throwing device 2 is activated, and the mixer motor 22 is maintained at about 3 rpm. »The culture tank 10 is placed in the cool place of the room, the contact area of the culture is carefully watched, the temperature of the culture medium is maintained at the optimal growth of the strain of Su bacterium, and the dish is 3 ft. 2c. When necessary, the heating device is turned on 4 冷却 or the cooling device 5 〇 adjusts the temperature. . There is no other control during the cultivation process, such as pH, pro- and dissolved oxygen control. The cultivation process can be completed in about three days. The culture solution collected from the product outlet 16 after completion can be used immediately, or the culture solution can be refrigerated and reused in the future. Based on the above remuneration, the culture method of the present invention includes:
㈠培養^沸滅菌：將培養基置於該培養槽10内，並加熱素沸； (一)導入二氣.在培養基煮沸滅菌結束前(例如前Μ分鐘）導人空氣於煮 弗之口養基中，该空氣係經該設於培養槽1〇 Θ之空氣過遽器36 ; (三）冷卻培養基； ⑻無雜菌接種：將種菌液於無_狀態下倒人培養槽1G中完成接種； :將雜養槽1G之排氣口蓋14打開―部份，維持無菌空氣自開口不斷排 出的狀態下，將種·倒人該培養槽1()中完成接種； 盖正M」於培養操作綱；即以持料人除菌空氣，使得該培 曰”、、有無菌空氣自培養槽排出而無外界含雜菌空氣可進入的狀 態，以減低受污染的機會。 4上所述’本發嗎錢於—種可滅狀簡倾生物培養設備及其培 於3槽=包;Ϊ養槽上方具有—排氣口 ；—通氣管穿設 管藉由一空_縮機通入一氣體，氣體經由排氣口 L 設於培養槽内並與通氣管相接S。如 t菌將空氣過濾器内的細菌消除，並藉由通氣管通入氣體再經^ 入ί絲的方式，可使培養槽内之空氣不斷排出，以避免細菌由外界進 紅微生物。教树縣構鮮，製作成本低廉， 讓使用者W域接料自行鱗微生物而使用。 故本發明實為一具有新穎性、進步性及可供產業利用者，應符合我國 7 201043692 專利法所規定之專獅請要件無疑，銳_ 早日賜准專利，至感為禱。 月專利申請，析釣局 惟以上所述者，僅為本發狀-較佳魏細已 明實施之翻，舉凡依本制_魏_述、、定本發 砷所為之鱗娜飾，馳包胁本發批中鱗概圍内。 【圖式簡單說明】 第一圖為本發佳實施例之可_之簡便微生物培養設備之側視圖；以 及 第二圖為本發明較佳實施例之可滅菌之簡便微生物培養設備之上視圖。 【主要元件符號說明】 10 培養槽 12 排氣口 14 排氣口蓋 142 氣孔 16 產品流出口 20 攪拌裝置 22 攪拌馬達 24 攪拌軸 26 攪拌葉片 30 通氣管 32 入風口 34 出風口 36 空氣過濾器 38 氣泡石 40 加熱裝置 50 冷卻裝置 201043692 52 冷卻管 522 輸入端 524 輸出端 60 溫度計 〇 ❹ 9(1) culture boiling boiling sterilization: the culture medium is placed in the culture tank 10, and the heating is boiled; (1) introducing two gas. Before the end of the culture medium boiling sterilization (for example, before and after the minute), the air is introduced into the mouth of the boil The air is passed through the air filter 36 disposed in the culture tank; (3) cooling the medium; (8) no inoculation: the inoculum is inoculated into the culture tank 1G without the state to complete the inoculation; : Opening the vent cover 14 of the mixing tank 1G to maintain the sterile air continuously discharged from the opening, and injecting the seed into the culture tank 1 () to complete the inoculation; Covering the M" in the culture operation That is, the carrier removes the sterilizing air, so that the culture is discharged from the culture tank without the outside air containing the bacteria and air to enter, so as to reduce the chance of being contaminated. The money is in a kind of destructible simple tilting biological culture equipment and its cultivation in 3 tank = package; the top of the cultivating tank has an exhaust port; the vent pipe is provided with a gas through a space-shrinking machine, gas It is disposed in the culture tank through the exhaust port L and is connected to the snorkel S. If the bacteria are inside the air filter The bacteria are eliminated, and the air in the culture tank is continuously discharged by the gas passing through the vent pipe and then passed through the wire to prevent the bacteria from entering the red microorganisms from the outside. The teaching tree is fresh and the production cost is low. The user W field is used to pick up the self-scale microbes. Therefore, the present invention is novel, progressive, and available for industrial use, and should conform to the requirements of the lion's stipulations in the patent law of China 2010. Granting a patent, the feeling is a prayer. The monthly patent application, the analysis of the fishing bureau, only the above, only for the hairline - the best Wei fine has been implemented, according to the system _Wei _,, the book The arsenic is made of scales, and the package is covered by the scales in the batch. [Simplified illustration] The first figure is a side view of the simple microbial culture equipment of the best example; and the second figure A top view of a sterilizable simple microbial culture apparatus according to a preferred embodiment of the present invention. [Main component symbol description] 10 Culture tank 12 Exhaust port 14 Exhaust port cover 142 Air hole 16 Product outlet 20 Stirring device 22 Stirring motor 24 Stirring Agitating blade 30 through the shaft 26 into the pipe 32 outlet 36 outlet 34 air filter 38 of the air stone 40 20104369252 condenser heating means 522 input terminal 524 output terminal 50 of the cooling apparatus 60 billion thermometer ❹ 9