TW201036551A - New pseudomonas bacterium - Google Patents

Abstract

To provide a method for controlling diseases caused by bacteria, fungi or viruses in a honeybee, an animal or a vegetable, and to provide a method for promoting the growth of the honeybee, the animal or the vegetable. The bacterium belongs to Pseudomonas fons, and has ability to inhibit the infection with the bacteria, the fungi and the viruses, and the proliferation thereof in the honeybee, the animal or the plant. The agent for inhibiting the infection with the pathogenic bacteria, fungi or viruses, and the proliferation thereof contains the bacterium as an active ingredient. Especially, Pseudomonas fons MS-1 strain (accession number: FERM P-21673) is preferable as the new Pseudomonas bacterium.

Description

201036551 VI. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a novel pseudo-single fetus + + early-genus bacteria using the novel pseudo-single

The genus of bacteria of the genus Bacteria, the _ plant early I # I x the general method, the method of earning the disease, and the method of cultivating the sputum. [Prior Art] 〇 〇 〇 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在 在. The fine handle is used to produce honey. So far, the Japanese honey bee is 4', but this variety is difficult to raise due to its mobile habit. At present, western honey bee is the main variety in honey production. For western honeybees, 'large losses are caused by the occurrence of several diseases. In the pot, it is caused by the rot of the rot disease (Mells_cus plutonius, the American genus paenibaeillus larvae), which is transmitted to the young bee by the bee eater and causes the disease in the bee larvae. Caused a large number of deaths. The above-mentioned diseases are caused by the method of medicinal feeding of silkworms, and the use of antibiotics (milomicin)/Piten", however, is produced in the countermeasures for preventing diseases. There is an event in which the above meaning is mixed into honey by means of a bee, and the purity of the f. In addition, the white mites caused by fungi (ascosphaeraapis) are highly infected in the bee larvae of 3 to 5 3 201036551, resulting in a large number of deaths, but antibiotics have no effect on fungi #, an effective solution to the disease Very few. In addition, the occurrence of disease in the bees in recent years has led to the disappearance of the entire bee colony. The above situation is called a bee colony dissipating syndrome, and acute paralysis becomes a deadly virus. No effective agent has been found against the above viral diseases. In the social tendency to suppress the use of pharmaceuticals in food materials in recent years, it is necessary to establish a bee feeding method that does not use a drug because the social level of the residual vitamins in the bee weight is improved. On the other hand, the diseases of livestock and vegetables are gradually expanding, and the use of new types of medicines is formed, although it is a disease or a disease. The reason for the enlargement of the above-mentioned diseases is exemplified by the imbalance of microorganisms in the digestive tract of the animal or in the soil (4) due to the use of a large amount of the medicament. For example, in nature: the virus is inhibited by bacteria, but the bacteria are reduced by daily antibiotics, so the virus liberated from the inhibition of bacteria is increased. The above phenomenon is prominently manifested in the antibiotics of the gorge (the growth promotion ship's livestock series. Therefore, it must be converted to a production method that does not depend on _. This method uses pathogenic bacteria, pathogenic fungi that inhibit the cause of the above diseases, Since the pathogenic virus is proliferated and infected with a fine g to prevent disease, the drug can be reduced and suppressed by using the above-mentioned useful bacteria. The microorganisms are used to add a nutrient-containing liquid medium to the body towel. The culture is carried out, and the ratio of the liquid to the medium is (10): 1 or less in consideration of the (four) in the liquid. However, in the case of the above-mentioned liquid clock culture, the amount of bacteria per unit of 35,651,51 ml is less, and the maximum harvest is obtained. The amount is from 1.09 to 101. The bacteria is finely divided into cells/ml, and in the case of administration to animals and plants, microorganisms must be cultured in a large amount. Therefore, it is necessary to develop a culture method for greatly increasing the amount of bacteria harvested. The problem to be solved is to provide a method for controlling bacteria, fungi or viruses which are used as a cause of removing bees, animals and vegetables, and Into the way of growth of bees, cockroaches, and vegetables. Surprisingly, the inventor of this case proposed that by containing Pseudomonas von (Pseudomonas 丨 丨 ( (Certificate No. FERMp_21673), it is fresh The present invention contains the following invention. The present invention contains the following inventions: 〇(1)-species(4), which has the ability to suppress the infection and proliferation of honey, hand animals or plants. 'The system is a pseudo-single-song rhyme (PSeud_as f〇ns), which has the ability to control the infection and proliferation of bees, animals or plants by fine H, fungi and viruses. (2) The bacteria in (1) are false. Monocytogenes • Von strain (commission number FERM P-21673) or its variants. (3) - a pathogenic substance _ bees, animal or plant inhibitors of infection and proliferation, containing (1) or (2) An inhibitor of infection or proliferation of a strong, bee, animal or plant that is a fungus that contains (1) or (2) bacteria. 201036551 (5) A bee, animal or plant infection of a pathogenic virus and An inhibitor of proliferation, which is a bacterium containing (1) or (2). (6) A growth promoter for bees or animals, which is a bacterium containing (1) or (2). (7) A growth promoter for plants, which is a bacterium containing (丨) or (2). A control agent for bees, animals or plants that is afflicted with diseases of bacteria, fungi or viruses, which is a bacterium containing (1) or (2). (9) - Feeds and strips or _ and food additives for bees or animals A bacterium containing (1) or (2). ' (10) A fertilizer or fertilizer additive, which is a bacterium containing (1) or (2). ((1) A cultivar of the fine g of the species (1) or (2), characterized in that it is used by The solid powder is prepared by cultivating the powder. By using the novel Pseudomonas bacteria of the present invention, it is possible to prevent bees, animals, and the main diseases of the disease, namely, rot, and white mites. In addition to the bacterial disease and avian influenza virus, the effect of increasing the production rate of honey is greatly increased. ^·The growth promotion effect of animals and vegetables that have been obtained in the country. Moreover, the above results are also indicative of healthy foods for the human body. , : The spread of diseases such as rickets, blight, and diseases. According to the novel Pseudomonas bacterium of the present invention, the above-mentioned bacteria can be obtained at a high yield in the case of use as an animal medicine or a case of a single-handed bacterium of the compassionate 1 Π 、, 八细固'. [Embodiment] 201036551 The present invention will be described in more detail below. 1. Novel bacteria (4) Pseudomonas sputum is a single singularity, and there is no particular limitation on bacteria which have the ability to inhibit pathogenic bacteria, fungi and viruses to inhibit infection and proliferation of bees, animals or plants. . For "animals" and "plants", the following valleys are described. Examples of bacteria which inhibit infection and proliferation by the novel Pseudomonas bacteria of the present invention include MeliSScxx_s or

Paenibacillus) (Salmonella infantis) ' Sl sputum sputum (Ralstonia solanacearum), Streptomyces scabies sacidiscabies, examples of fungi can be listed as the original fungus (Ascosphaera apis), examples of viruses can be listed as avian influenza virus (H3N8) , flounder, sputum, sputum, and rotavirus, but it is not limited thereto. The bacterium having the above-described ability is preferably Pseudomonas fons MS-l strain (commission number withdrawal M p_21673), but is not limited thereto, and for example, a gene belonging to 16SrRNA can be used. Partially arranged to arrange the pseudomons g of the number 1 of Table 7F; Mas, and the bacteria having the bacteriological properties of the town and classified by Pseudomonas vons. Shape: Bacillus; Length: 1~3 μm; Mobility: Yes; Pore formation: Innocent Gram stain: Negative; 0F test: - Fertility level after 1~2 days of culture to form a population of _, color: Thin brown; luster. No special; surface: smooth: diffusive pigment: no; pH: 4. 〇(—), 5· 〇( + ), 7 201036551 5. 6( + ), 8· 6( + ), 10· 〇 ( + ) Temperature: medium temperature 7~45t, pool with ·· 〇~4% lithic acid reduction: ~; denitrification reaction: -; ah: -; sulphide chloride: -; -; Acid salt utilization: ~; Pigment production: -; Urease: -; Oxidase: +; Salt catalase:; DNA G + C; Content: 68 mol% In addition to the use of bacteria and fungi that inhibit pathogenicity And the virus on the bees, animals or plants * and & ... the bacteria of the force, can also be better use of Pseudomonas • Codes MS-1 strain variants. Here, the mutant strain of the mutant Pseudomonas sp. (4) strain is induced to undergo mutation. Variant inducing treatment can be obtained using any suitable variant. Here, as the word "original", it should be understood that, in a broad sense, for example, in addition to the effect of the mutated original, it also includes the mutated effect when the UV irradiation is performed. Examples thereof include ethyl methyl sulphate, UV irradiation, methyl ketone, fish p, ketone + nitrosoniamitine diamine, and a nucleus base such as bromo broth. For the cultivation of the novel pseudo-monoculture of the soybean powder _ g and the rice genus bacteria of the present invention, solid 1 〇〇〇 ml ^ ... shoulder g, yeast extract 4 g can be used. , distilled water water ml and the generality of the _ can be made ± iL Yiqi ς ^ < soil, and no special composition. For example, 1 for: 1 «apricot bg, yeast extract ml.nK7 9 7 , ^ g, dipotassium dish potassium G. 2g, steamed crane water 1000 muscle PH7. 2~7, 4 culture, * %. use. _ face 121.匚, 15 minutes of heat sterilization 201036551 • In addition, the culture capacity of the new Pseudomonas bacteria cultured in solid and liquid • The stomach sputum uses the secret micro-organism culture device, and there is no side limitation method. For example, in small-scale culture, test tubes, bottles, cultures, etc. can be used on a large scale. In the middle of the cultivation, fermenters, cans that can be ventilated, and the like can be used. ο It is a large amount of raw __, especially _, which is cultured from solid medium prepared from solid powder. Here, as the solid powder, soybean powder or rice bran powder may be used as a main material, and it is preferable to add a small amount of yeast extract and egg to make a mixture. Further, an inorganic substance such as an acid carrier can be used as the powder. The inventors of the present invention found that in the solid powder, 2Q m biga, (four) 丄, _ points were added per (10) g of the thermal age X, and the above-mentioned fine riding was inoculated, and the hunger was passed through for 3 days =: Adding 6" ι or more of the medium in the medium, fine (four) ❹ 疋 _ (four) reading _ g water content is 8g ~ i4q W. In addition, even: the coffee inorganic particles (granules pinch about 1 〇 _ number 丨, Pseudomonas is the bacterium of the genus Bacillus. The nutrient solution 1 L · t θ θ σ to 1 〇 times. Preferably, the number of the helmet particles I (4) is in the cultivating material 2 () ~ , " , culture, or addition of a solid powder to a liquid medium, the new fine fl and the sputum f 1 in the solids of the solids 1 'by the invention Mogu recognized: r L in the so-called solid attachment substance attached culture The situation = ^ bacterial yield than the scale of the liquid sugar liquid, _===== 9 201036551 Substance: the spread of Benxi to the environment of bees, animals or plants. H is the same as == cells or cultures or their treatments, etc. · Here, t is: ^ fine (four) whip tearing _, no bacteria refers to dilute: =:? The concept of rationality, for example, also & release, reduction, dry matter, east knot, etc. Under the state of direct 彳μ目 in the form of the body culture and bacteria that can be cut and cut. The bacteria of the invention can be kept at a storage temperature of 5 ΐ or less for about 3 weeks (4), and can be kept and cultivated by a low-temperature shirt 2~ bee, animal or plant. Weng can be effectively utilized for honey-to-yellow, bundle-cultured bacteria. The body culture is divided into sugar, and the sugar is frozen and the battalion is long, and the addition of glucose or cane knot is effective. By using Kang, α buttocks, it is still _20 for a long time. Feeding bees, animals or plants for more than a month, and ending the training, and set up a W. 而 而 ❸ ❸ ❸ ❸ ❸ ❸ ❸ ❸ ❸ ❸ ❸ ❸ 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效 有效Long-term storage and observation can be used for animals and plants - the plate is suitable for the age. But for the age, but can make the Qing „ U not limited to too smoke · vacuum view drying, etc. x ', bacterial culture, body, or resurrection (For example, _ use, feed _ ten with 4 umbrella treatment, can be used with the way, animal purchase, aquatic _) in the middle: the way, the use of fertilizer, the excipient or carrier of the medical license, and make 201036551 ^ for example, the age of H can be combined with touch, such as fishmeal, glutinous powder, seaweed powder, inorganic powder, And if it is = the intended use, it can be of any kind. And the right is the novel Pseudomonas bacteria and the white mites + bee mites that can be used in the present invention (especially rot ❹ Ο ::: The poisonous r, ·) ^ The seven characters are valid 1 of the new Pseudomonas is a special test - ·, can be listed as a paste of the larvae of the bee,: ί Add a new type of pseudomonas bacteria for feeding In the method, a method of spraying a liquid of a new type of sclerotium eyebrows, a liquid of a bacterium of 11 genus (for example, a dilution of a culture), and the like are sprayed. In particular, it is desirable to culture the novel Pseudomonas bacteria in a solid medium formulated with protein peptone, yeast extract, and soy protein gland, and to dilute the transgenic material by the nuclear filaments. Doing so that the bee larvae feed. Advantageously, the larval disease is reduced by the administration of the novel Pseudomonas bacteria, and the survival rate of the larvae is increased, so that the number of bees can be greatly increased, which can be confirmed in the examples. Use of the novel Pseudomonas bacteria of the present invention in the prevention of diseases in animals (for example, 'the sputum caused by the sputum g . "Listening, even, and removing" includes treatment, noodles, and inhibition. 201036551 p The present invention relates to a method comprising the steps of administering an effective amount of a bacterium to an animal, and preventing the disease of being true and wide. The so-called "animal" = disease: disease (4) poultry (such as poultry), M pure scorpion (such as 'bovine, pig), pets, animals, the method of administration, there is no special genus Pseudomonas bacteria to make livestock Method of feeding ^ Adding a new _ method containing a liquid of a novel Pseudomonas bacterium, etc. == In a healthy food of the human body. . In the case of the effect, the novel Pseudomonas bacterium of the present invention can also be used for controlling diseases in plants (for example, Fan Li: Bacterial wilt, taro-like sore touch _. Here, the so-called "anti-w The concept of treatment, and inhibition, that is, the efficacy of the new Pseudomonas bacteria applied to the plant step 2 == plants - the so-called "plant two shells 2, specifically, _ , pepper, broccoli, taro-like sulphur rice. Method for applying an effective amount of a novel Pseudomonas bacterium to vegetables = ship, for example, _ _ _ single _ bacteria mixed ^ soil shot method, And the method of applying to the plant body, and the method of licking the seed, in addition, the present invention contains an effective amount of the new Pseudomonas sp. 201036551 bacteria applied to the plant and promotes the product. The method has been confirmed to have the above-described growth promoting effect in (4), pepper, and broccoli = the application method of the plant is carried out as described above. [Example 1] fe early cell _ · is MS-1 strain Isolation and homology 1 · The strain is isolated from the freshwater environment' and has The following describes the characteristics of 形状 形状 杆菌, length: 1~3 microns, motility: yes, neurite formation: Yi's staining: negative, 0F test: - '1 3 · Fertility degree after 1~2 days of culture formation Group, color: thin brown; luster. No special; smooth surface; diffusible pigment: no; pH: 4. 〇(-), 5. 〇( + ), 8.6( + ), 9 ·0( + ) , 1〇, 0( + ); Temperature: Moderate temperature: 〇~4% 4. Reduction of nitric acid: Denitrification reaction: 吲哚:-, hydrogen sulfide: _ citric acid: -, nitrate utilization: -, Pigment production: -, urease: _, oxidase: +, medium: +, DNAG + C content: 68 mol · % 5.16 SrRNA analysis

After the nucleic acid was extracted using a Sepa Gene kit (manufactured by Sanko Pure Chemical Industries, Ltd.), the nucleic acid was recovered by ethanol precipitation. The use of two specific primers for 16SrRNA did not produce a towel! Then, the green inspection (made by Pharmacia) 201036551 was carried out. For the purified PCR amplification product, the salt base alignment was resolved by applying the biological system 377. Thereafter, online retrieval was performed by the database of the sheenbank. Hereinafter, the salt group arrangement of the 16SrRNA gene of the Pseudomonas fons MS-1 strain is shown as the corresponding DNA data (Table 1). According to the industrial category review benchmark, the microbial industry was applied, and the new version of the bacteria (edited by the Patent Office) was used to investigate the traits of the bacteria, and the taxonomic position was discussed based on Baumann (1984). The result was There is no documented strain corresponding to this strain, so it was named Pseudomonas fons MS-1 strain. Invented by the inventor of the present invention, the strain was named Pseudomonas and Von MS-1, and was commissioned as a patented biological entrustment center of the Industrial Technology Research Institute of the Independent Administrative Corporation as an entrusted number FERM-21673 on September 18, 2008. Trusted. [Table 1] Pseudomonas • Von MS-1 strain 16S ribosomal RN gene, partial sequence length - 1531 score = 2773 bits (1399) E value = 0, 0 identifier = 1402/1403 (99% ); phosphoribosylglycinamide synthase 20/1403 (0%) bond = positive / positive

Pseudomonas fons MS-1 16S ribosomal DNA gene, partial sequence Length=1531

Score = 2773 bits (1399), Expect - 0.0 Identities - 1402/1403 (99%), Gaps - 0/1403 (03⁄4)

Strand=Plus/Plus 201036551

Ο

Query 9 Sbjct 67 Query 69 Sbjct 127 Query 129 Sbjct 187 Query 189 Sbjct 247 Query 249 Sbjct 307 Query 309 Sbjct 367 Query 369 Sbjct 427 Query 429 Sbjct 487 Query 489 Sbjct 547 Query 549 Sbjct 607 Query 609 Sbjct 667 TGAGTGGAGCTTGCTCCATGATTCAGCGGCGCACGGGTGAGTAATGCCTAGGAATCTGCC 68 iimmimimimm Shu Hmmim: Shu miimmmmiim TGAGTGGAGCTTGCTCCATGATTCAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCC 126 TGGTAGTGGGGGATAACGTTTCGAAAGGAACGC \ ATACCGCATACGTCCTACGGGAGAA 128 Ι! ΕΜ! ΙΙΙΙ !! Μ! ΙΙ1! ϋΙΙίΜ !!!! ΜΜ !! ΜΜΜ1ί! Μ! Μ !! Ι! ΙΙΙ! 1! TGGTAGTGGGGGATAACGTTTCGAAAGGAACGCT.HTACCGCATACGTCCTACGGGAGAA 186 AGTGGGGG ATCTTCGG ACCTCACGCT ATC AG AT ·:: .GCCTAGGTCGG ATT AGCT AGTTGGT 18 8 mmiimmimimiimm丨丨iiiNiimim AGTGGGGGATCTTCGGACCTCACGCT/lTCAi'.ilv;AGCCTA,GGTCGGATTAGCTAGTTGGT 246 GGGGTAATGGCCCACCAAGGCGACGATCCGTiV:Ti;GTCTGAGAGGATGATCAGTCACAC 248 11!!ΙΙΙ!!Ι!Ι!ΜΜΙ!!!ίΙ!Μρΐ! ΜΜΜ!ιί!Μ!!!Μ!Ι!ΙΙΙ!ί!Μ! GGGGTAATGGCCCACCAAGGCGACGATCCGTAA^FGGTCTGAGAGGATGATCAGTCACAC 306 TGGAACTGAGACACGGTCCA GACTCCTACGGGACGCASCAGTGGGGAATATTGGACAATG 308 iimimimimimimmimm Shu mNiimmimimiii TGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATG 366 GGCGAAAGCCTGATCCAGCCATGCCGCbTGTG :: > '\ ^ AAGGTCTTCGGATTGTAAAGCAC 368 111 I Μ 1111111 I !! I i !! Π Μ Μ Μ; M:!!! M: i η !! π I ί 11 ί! ! ί ί I I i M GGCGAAAGCCTGATCCACCCATGCCGCGTGTG'rG'.AGUGGTCTTCGGATTGTAAAGCAC 426 TTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTTTTGACGnACCAACAGAA 428 mmmmiim mmmmmMuuu Shu mmmmmm TTTAAGTTGGGAGGAAGAGCAGTAAGTTMTACC'TGCTGTnTGACGTTACCAACAGAA 486 TAAGCACCGGCTAACTTCGTGCCAGCAGCCGCG ';! rAAT.ACGAAGGGTGCAAGCGTTAATC 488 TAAGCACCGGCTAACTTCGTGCCAGCAGCCGCGGFAATACGAAGGGTGCAAGCGTTAATC 546 GGAATTACTGGGCGTAAAGCGCGCfrFAGGTGGTTCAGC / UGTTGGATGTGAAAGCCCCGG 548 l lll !! ll l !! IMiM!!! !li!!:i:iiM. MhiMIMNiiMliiMNii! GGAATTACTGGGCGTAAAGCGCGCGT-1GGTGGT ' AG;:AAGTTGGATGTGAAAGCCCCGG 606 GCTCAACCTGGGAAnGCATCCAAAACTACTGA ICTAGAGTACGGTAGAGGGTGGTGGAA 608 !丨|丨丨丨丨|丨丨丨丨丨丨丨1丨丨丨丨丨丨丨mmmimm GCTCAACC TGGGAATTGCATCCAAAACTACTGA1(:TA(;AGTACGGTAGAGGGTGGTGGAA 666 TnCCTGTGTAGCGGTGAAATGCGTAGATATMG\AGf;AACACCAGTGGCGAAGGCGACC 668 TTTCCTGTGTAGCGGTGAAATGCGTAG.\TAT \G\Λ(;(;ΛACACCAGTGGCGAAGGCGACC 726 201036551

Query 669 ACCTGGACTGATACTGACACTGAGGTGCGAAAGC5''GGGGAGCAAACAGGATTAGATACC 728 111II! 11!! 111III i I Μ IIIII!!!!!! I! ί 11! m Μ ! Μ! I! 11111! 11! i 11

Sbjct 727 ACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACC 786

Query 729 CTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCG'n'GGGATCCTTGAGATCTTAGTGG 788 mmimmmmmmmmm丨...mimmmmmiij

Sbjct 787 CTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCGTTGGGATCCTTGAGATCTTAGTGG 846

Query 789 CGCAGCTAACGCGATAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAAT 848

111!!! 111111 Μ III1111!! 11 M !!!!! i! i 111 i I! HI I Μ III111!!! 11! I

Sbjct 847 CGCAGCTAACGCGATAAGTCGACCGCCTGGGGAGl'iiC^GCCGCAAGGnAAAACTCAAAT 906

Query 849 GAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTCGTTTAM'TCGAAGCAACGCGAAG 908 ΙΙΙΙΙΙΙΙΙΙΙ!ΜΙΙΙΙΙΙΙΙΙΙΙΙίΠΙίΐ!Μΐ!!|ΐΙ!!!|||!!ΙΙΙΙΙΙΙΙΙΙΙ Sbjct 907 GAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTHAATTCGAAGCAACGCGAAG 966

Query 909 AACCTTACCTGGCCTTGACATGCAGAGAACTTTCCAG.AGATGGAnGGTGCCTTCGGGAA 968 iiiiiiiiiiiiiiiiiiiiiiiiiMiiiiimmimmiiimiimiiii

Sbjct 967 AACCTTACCTGGCCnGACATGeAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAA 1026 Query 969 CTCTGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC 1028

MiiiiiiiiiiMmmiMmmmmmmiiiimiMiiiMim

Sbjct 1027 CTCTGACACAGGTGCTGCATGGCTGTCGTCAGCFCGTGTCGTGAGATGTTGGGnAAGTC 1086

Query 1029 CCGTAACGAGCGCAACCCTTGTCCTTAGTTACC\G(:A(XTCGGGTGGGCACTCTAAGGAG 1088 11111 m m 11 m μ 丨 I m I m_ ί 丨丨ί 丨 m m m m I m 丨丨 I 丨 m in I i

Sbjct ] 087 CCGTAACGAGCGCAACCCTTGTCCTTAGTTACC;\G(:ACCTCGG(;TGGGCACTCTAAGGAG 1146

Query 1089 ACTGCCGGTGACAAACCGGAGGAAGGTGGCG^TGWXil'CAAGTCATCWGGCCCTTACGG 1148 I!! III1111 Μ 1111111111111!! Μ i!!! Μ ί ί ί! Μ 1111! i 111 III 1111! II '

Sbjct 1147 ACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGG 1206

Query 1149 CCAGGGCTACACACGTGCTACAATGGTCGGTA(;.v\/\(;GG'rTGCCAA(;CCGCGAGGTGGAG 1208 !ΙΙΜ!ΙΙΙ!ΜΜΜΙ!ΜΙΙΙΙί!!!Μ!ίΗ;)!!! ί!ΙΙ!!Ι!1ΙΙ!!Ι! ΙΜ!ΙΙ '

Sbjct 1207 CCAGGGCTACACACGTGCTACAATGGTCGGTA(;:\AAGGCTTGCCAAGCCGCGAGGTGGAG 1266

Query 1209 CTAATCCCATAAAACCGATCGTAGTCCGGAUX'CAGTCTGCAACTCGACTGCGTGAAGTC 1268 111 III 1111! II! 1111II! III i Μ ! I! i I ί i! !M! II! Μ 111 III I! II III 111

Sbjct 1267 CTAATCCCATAAAACCGATCGTAGltX; (X; ATrGr: AG'f(;TGCAACTCGACTGCGTGAAGTC 1326

Query 1269 GGAATCGCTAGTAATCGTGAATCA(;AA1'G1C/\C( ;(;TG1ATACGTTCCCGGGCCTTGTACA 1328 iimmmmmmm!丨!丨nmu丨

Sb jet 1327 GGAATCGCTAGTAATCGTGAATCAGAATGT(:;\i:!iGT(;\AT,\CGTTCCCGCGCCTTGTACA 1386 1388 201036551 1446

Query 1329 CACCGCCCGTCACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGTCTAACCGCAAGGA 丨丨丨丨1丨丨丨丨丨丨丨丨丨丨丨丨丨丨丨mmiimm丨丨丨丨丨丨丨|丨丨丨丨丨丨丨丨丨丨丨丨丨丨丨丨丨Sbjet 1387 CACCGCCCGTCACACCATGGGAGTGGGnGCTCCAGAAGTAGCTAGTCTAACCGCAAGGA

Query 1389 GGACGGnACCACGGAGTGATTC 1411 NIMIMIMMMIIIIIMI Sbjct 1447 GGACGGnACCACGGAGTGAnC 1469 [Example 2]

〇 b Under the department, recognize the secret list. The lying MS-ι strain has the ability to inhibit the proliferation of pathogenic bacteria. The smear specimens of the two upper-stage plants pre-applied on the agar medium were transplanted with Salm〇nella infantis, Rhizoctonia solani and S. lanacearum, and cultured under the muscle for 1 day, after which the specimen was smeared. The population size between the pathogens is compared with the population size in the case where the disease is cultured only as a lamella, and as shown in Table 2, the proliferation of the Salmonella g and the Phytophthora can be inhibited. "Suppression" is the ratio of the size of the banner of the population of pathogens on the medium of the simplification rate to the same size as the population of the test area.

Further, the ability of the above-mentioned Pseudomonas, Masis H ^^^#f.J4^(Melissococcus plutonius ^ Paenibacillus larvae) and A. sphaera apis to proliferate can be confirmed by the same method. [Table 2] Fungus name

Melissoccoccus plutonius inhibition rate (%)

Paenibacillus larvae inhibition rate (%)

Ascosphaera apis inhibition rate (%) 17 201036551

In the natural world, there are a large number of infections that inhibit the virus, or the virus is decomposed. ★ The bacteria are in the natural world as above. If antibiotics are used, the antiviral fines are suppressed. On the other hand, for most antibiotics, the disease = remain resistant. As a result of the above, the antiviral can be finely eliminated by the use of the hopping hormone, so that the part of the disease is fully infected and proliferated. In the case of beekeeping, the use of antibiotics will eliminate the rot pathogens, and in addition, the possibility of money and money will increase. The same scenario has occurred in the breeding of porpoise, poultry, cattle and fish. Therefore, in the present study, the virus inhibition of the useful fine_MS-1 tissue was measured. As described below, it can be confirmed that the above-mentioned strain Pseudomonas s. g. sales ms-1 has an ability to inhibit the growth of pathogenic scales. In addition, because it is still unable to culture the virus caused by the disease of bees, it is a virus that maintains the similarity of the outer skin (ca_). The system uses 18 201036551 county (_8) and (4) hematopoietic transgenic silk virus (IHNV) as a control virus. That is, the inactivation of the virus is caused by the decomposition and damage of the virus skin. Therefore, the result of this experiment is that the virus which causes the disease of the bee is more inactivated by the virus. First, in the culture system of the MDCK cells in the kidney, whether or not the culture supernatant has a feeling of avian influenza virus (BUD/deletion), which is a pathogenic virus for inhibiting avian influenza, is determined (4). The effect of inhibition is to inhibit the effect of canine kidney and visceral cell (10). That is, the canine kidney MDCK cells were placed in a liquid medium of 1 mi (containing recommended bovine serum components, 〇·〇75% NaHC〇3, 1〇〇1_penicillin, 1〇〇//g/ml chain microtin, κ 6% TriS-HCl (pH 7.8) contains), at 25. (: The culture was carried out, and a culture solution of the avian influenza virus (10) N8) (g. 1 ml) and the culture supernatant of the above Pseudomonas strain MS-1 (〇. i ml) were added to the culture system. As a control experiment, only a culture solution (〇·1 ml) of avian influenza virus (BUD/H3M8 strain) was added to the above culture system. Here, the culture supernatant of the above pseudomonomer__ is said to be cultured in the above fine medium at 25 C for 3 days, and then subjected to spin-down (4 rpm) to precipitate the cells, and borrowed The filtrate was obtained by filtration through a 0.22 /zm filter. In addition, as a culture solution of the avian influenza virus (BUD/H3N8 strain), the avian influenza virus (H3N8) is propagated by using the same culture solution and the kidney MDCK cells of the dog, and then filtered by a filter of 0.45 _ And the filtrate obtained. The CPE after the addition of the above cell culture supernatant was tested over time. The lower case of 〇^ means that the proliferation of the virus is inhibited. The results are shown in Table 3, respectively. 19 201036551 In the case of the culture supernatant to which the Pseudomonas Von MS-1 strain was added, the proliferation of the avian influenza virus (BUD/H3N8 strain) was inhibited. Further, by changing one of the above methods, it was confirmed that Pseudomonas von V. MS-1 strain has the ability to inhibit infection and proliferation of infectious hematopoietic necrosis virus (mNV). That is, in the above method, salmon cells (IHV) were used instead of avian influenza virus (H3N8)' and investigation was carried out using infectious hematopoietic necrosis virus (IHNV), and as a result, Pseudomonas bacteria- The culture supernatant of the sputum strain inhibited the proliferation of infectious hematopoietic necrosis virus (腑) (Table 3). Furthermore, the ability of the virus appears as the culture cell infection price (TCIDso). [Table 3] Avian influenza virus (BUD/H3N8) Infectious hematopoietic necrosis virus control area l〇5'8TCID5〇/25ul 10S7TCID5〇/25ul MS-1 strain 1〇i'9TCID5〇/25u1 1〇15TCIDm/25u1 [Example 4] It is a bacterial effect in the lock system (4) Three net chambers are provided, and a total of three bee colonies of the bees of the bees are individually introduced into the respective net chambers and turned over. In the beehive of one of the three bee colonies, stealing _ called Zhu (for group workers), and for other i 4 apis group (four) strains, and mixing them to the sugar as the bee's material In the liquid (injection group E). The remaining bee colony is used as an attack control group and has not been invested. 20 201036551

Sputum. During the (10) strain, during the administration period, all three bee colonies were attacked with the rot disease, and the disease of the stagnation disease and the status of the bee colony within 5 encounters after the attack were observed (feed intake, seeding, scales (4) and scales). Activities depend on). As a result, there was no difference in the three bee colonies for the feed intake. Moreover, after the attack of the pathogen of the rot sister, the mild scaly temporary larvae were common among the three bee colonies, and the number thereof was somewhat reduced, but no rot disease was found in the milk-strain plant. A disorder is produced in the group, and a disease is produced in the challenge control group. That is, no rot disease has been found in the two bee colonies that have used the bacteria. As can be seen from the above, the disease of the rot disease is effectively controlled by the useful bacteria (4) in the bee colony (Table 4). [Table 4] Test group - control method control group -~~------- will be the enemy T sucrose liquid 匕d 33⁄4 then the number of the sisters of the test 16 ~ 1 and 1 voted with group E -- ------ Spray MS-1 strain dilution " ---------- In the rice (gauze broadcast you Λ, "A a 0, do not Λ 尔 尔 J J mixed MS-1 Iron 0 '丨-~--

[Example 5] The disease condition of the white ridge was prevented by the pseudo-bacteria•Ms-m. As a result, the value of the seizure of the smear to the suppression trace 5 was expressed as the value obtained by averaging the number of 4 boxes in each experimental area by 21 201036551 [Table 5] With the strain ~ administered group I mouth mist MS-1 strain dilution of the number of bee stings of white disease / beehive 3 8 0 ^ 5 2 [Example 6] long promotion effect for the upper secret high anti-8 force Bianzhu, mix it with the cultured liquid, and transfer it to the honey, and cast it into the honey. The sales were about 2 months. The experimental site was located in the town of Miyazaki, Miyazaki Prefecture, and Kobayashi City, the same county. For the investment and harvesting of the bacteria (four) days, the bees were tested daily. As a result of the above-mentioned administration test, the song was set in the 6 caster _ investment zone, Qin Dongbi, and there was no problem in the health of the bees in all the strain-administered test areas, and it was found (Table 6). The number of bees in Table 6 indicates the average value of the nesting box (box). [Table 6]

Number of bees (after 5 weeks) 21, 000 26, 000 [Example 7] 22 201036551 - The ward of the plant's raising box is controlled by the 舆 县 县 对 对 早 早 早 早 早 早 早 早 早 早 早 早 早 早 早 早 早 早 早 早 早Nagoya Tooth Poultry), each area was placed in a single 6-day feeding trial for 13 days. The composition of each zone was increased by the amount of bacteria: 5% (Table 7-1), and no added bacteria (Table 7-2). As a basal feed, a non-mixed antibacterial diet (Balance up 18, manufactured by Sakamoto Preparation Co., Ltd.) was used. 〔 [Table 7 -~1] One of the Nagoya cross-toxins and eight-day-old poultry was kept, and 5% (liquid/weight) of Pseudomonas and Von MS-1 strain were added to the feed. 8 9 10 11 12 13 14 15 16 Weight (g/only) 80 92 98 104 109 115 120 125 130 Feeding amount (g/day/day) 10.0 10.6 11.2 11.8 12.3 12.9 13.1 13.8 14.0 〇 [Table 7-2] There were 10 Nagoya cross-toed poultry and 10 birds of 8-year-old poultry, and Pseudomonas·Mass MS-1 strain was not added to the feed. 8 9 10 11 12 13 14 15 16 Weight (g/only) 81 92 97 102 107 112 118 121 126 Quantitative amount (g/ 10.2 10.6 pcs/day) 11.4 11.8 12.4 13.1 13.4 13.9 14.3 Pseudomonas aeruginosa斯M, Sr丄迷^ 龠 龠 observation of the top of the eye 23 201036551 Appetite: good fecal traits: solid (good) Vitality: good feather luster, etc.: good accumulation of feed intake and Zhao; ^ Table 7- 1 and the table _2 (weight increases with good conditions) d test (sick females have no wide and no liver discoloration, lions, excess fat, no abdominal swelling, no kidney stones, etc., no intestinal wall abnormal disease control effect will be small birds 100 were divided into 2 groups, one of which was administered with Pseudomonas and the genus n strain, and the other group was not administered with the strain. Moreover, the pathogenic Salmonella infantis was mixed with the compound feed. In the small birds that were added to the Pseudomonas and Von MS-1 strains, the number of small birds that occurred due to Salmonella disease was significantly reduced (Table 8). [Table 8] The number of episodes of bacillary disease is only 5 (all 5 〇). MS-1 strain is not added to the copper material. MS-1 strain is added to the copper material. ~~~~ ^43 ~~_________------ 6 [Example 8] 24 201036551, ral's fake single Gu Fengsheng 1 culture medium and mixed calf for calf &=Qml ( Black hair and cows, about 30 days after birth, are divided into 3 groups, and the pair 2': (4) Pseudomonas cephalospores • Von MS-1 strain, and the second group

The result was that the symptoms of Pseudomonas sputum were significantly improved (Table 9). [Table 9] η ~·- The MS-1 strain was not added to the milk and the MS-1 strain was added to the milk. The plant was added once. 7 7 The milk was added to the MS-1 strain and administered twice~~~~~~~ ~-~~~~~— 9 -

[Example 9] Search for waste and fruit) (Huang 1 (^ 11) ^ Fanyou seedling 2G tree is divided into 2 groups and planted in 10 units. Secondly, Pseudomonas sinensis is mixed with soil. In the middle (_), and the shame is applied; the roots of the 10 groups of the seedlings of the group are not applied to the other group, and 'the growth of the above-mentioned Panyou 3 weeks later (Table 10). As a result, it was found that the growth of the genus of the genus of the bacterium, the rice plant, the MS-1 strain, was promoted. 25 201036551 [Table 10] — The diameter of the shaft (5 cm from the surface of the soil) MS-1 was not added to the soil. MS-1 strain was added to the soil of the plant - 38 cm 4. 0 mm 43 cm 4.5 mm. For the above 2 groups of the above 3 weeks (4), the bacterial liquid of the pathogen (Raist〇nia __fine) was eucellular/mi)2 〇 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投 投As a result, the occurrence of the disease was inhibited in the Puzhuang of the Pseudomonas von Von MS-1 strain (Table (1). [Table 11] ~~----- Number (all 10) Not added MS-1 strain 8 Add MS-1 strain 0 [Implementation column 10] 勉 New Qianpei test 120 pepper seedlings (average main stem length 1 〇.8 (10) per (9) For the unit / 7 for 2 groups, the next step is to mix Pseudomonas and Pharmaceutics (Na) in the soil and apply 25 § to the root ring of each of the 60 groups of peppers of the first group. The 6G of the second group was not _ strain. Moreover, the growth of the above cedar was measured after 4 weeks (Table 12). As a result, it was found that the administration of the fake _ · butterfly ms-1 can promote the growth of the pepper. [Table 12] 26 201036551 The length of the main stem (average of 60) The MS-1 strain was not added to the soil. 19. 2 cm The MS-1 strain was added to the soil. 26.2 cm [Example 11] About 200 seeds of the stalk of cobalt vine stalks are divided into 2 groups, and 1 丨 of the 丨 丨 group is placed in the state of the mesh of small mesh, impregnated with Pseudomonas • Code MS -1 strain of liquid for 3 minutes. For the second group of species About 100 seeds were not impregnated. The above seeds were placed on a water-containing sponge block and left for 2 weeks. As a result, it was found that the seeds of the broccoli from the sea bream block were distributed and the stems were stretched, so The length of the stem was measured, and the flower immersed in the seed of the pseudomonocyte g·tMS-1 strain was grown (4) (Table 13). [Table 13] Stem length of the broccoli (1 〇〇 Average) MS-1 strain 7.8 cm was not added MS-1 strain 9.1 cm was added [Example 12] 壬遽 之 戚砗 戚砗, pr except for 芋 类 (especially potato chips, nationwide due to actinomycetes (Streptomyces scabies, S. acidiscabies) causes the spread of scabs. Because of the above-mentioned diseases, the surface infection of the head and the hollow inside the intestines cause the production of steamed bread to be reduced and the taste is low, and the value of the commodity is reduced. On 27 201036551 In the case of this disease, the effect is low. Therefore, the planting is divided into two groups by planting 100 units, and mixed with the bacterium (10)) in the soil, such as: =. And applied 50g to the first, and the seeds of the horse potato, 100, applied Around the various gimmicks, there is no other group of plants. Μ, for the above scales, there are no diseases (3). The results can be given to the horses of Pseudomonas and T. In the bell, the occurrence of sore disease is suppressed. In addition, more than one word is added from the head of the seed. Therefore, the increase from H_芋 is 1 group, and it is touched by (10) There is a disease in the H00 group. [Table 14] Number of potatoes with scab disease / 1 No MS-1 strain added to the soil~-----_ _ 45 Addition in soil MS-1 strain--------. 6 ——— [Example 13] The solid culture method of the strain was made by mixing 800 g of large ugly powder, 2 g of rice bran, yeast extract, and distilled water to make money. Body culture, and 12 minutes of heat and 12 minutes of heat sterilization. In the above solid medium, 'MS-1 strain cultured in a liquid medium (soy protein gland (5), yeast extract 3 g, dish acid two clocks. 2 g, steamed water 1000 m bu PH7 · 2~7. 4) was added. The culture solution was mixed with 5 ml and cultured for * days. As a result, it was recorded as a solid-cultured MS-1 strain/g, which was 10 to 100 times the number of bacteria/g of 28 201036551 in liquid culture (Table 15) [Table 15] Number of bacteria of MS-1 (5 days later) 1〇9 ～1^^ cells/ml 1〇10 ～1〇12 cells/g

In addition, the medium can be cultured in a medium fine medium. For example, 'even if the soy protein gland is 5g, the yeast extract is used, the Wei two dew. U, the fine water surface ml, the ship 2~7 · 4 medium sharply added the number of 1 〇 g (example. 20~50g) Inorganic particles such as algae (the phytoplankton _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ It has also increased substantially. Similarly, in the case where particles of an inorganic substance such as zeolite were added, the amount of the cells was also increased in the same manner (Table 16). [Table 16] Culture method MS-1 number of bacteria (0 days) MS-1 bacteria number (after 5 days) Cultured by liquid medium 105 cells / ml 109 ~ 101 ° cells / ml by liquid medium + Culture of diatomaceous earth 105 cells / ml 101 ° ~ 1011 cells / ml

Culture method Liquid culture The number of bacteria in solid culture (〇1〇5 cells/ml_----- 105 cells/g [Simple description] [Main component symbol description] 29

Claims (1)

201036551 VII. Patent application scope: 1. The species is a Pseudomonas fons, which has the ability to inhibit pathogenic bacteria, fungi and viruses from infecting and proliferating bees, animals or plants. 2. The bacterium according to item 1 of the patent application is Pseudomonas von V. MS-1 (commission number FERM P-21673) or its variant. 3. A pathogenic bacterium against bees An inhibitor of infection or proliferation of an animal or plant, which comprises the bacterium according to claim 1 or 2. 4. A fungal fungus for the infection and proliferation of bees, animals or plants, such as The bacterium according to the first or second aspect of the invention, wherein the bacterium according to the first or second aspect of the patent application is the inhibitor of infection or proliferation of a bee, animal or plant of a pathogenic virus. 6. A growth promoter for a bee or an animal, which comprises the bacterium as described in claim 1 or 2. 7. The growth promoter of the plant, the application for the age of the _ _ 丨 or 纟8. A disease for a bee, an animal or a plant, which is caused by a bacterium, a fungus, or a virus. The agent 2 contains the bacterium as described in claim 1 or 2. = for a bee or animal shop And food or shop and food additives, such as patent application The bacterium according to the first or second aspect. 10. A fertilizer or fertilizer additive comprising the bacterium as described in claim 1 or 2 of claim 30 201036551. 11. A method as claimed in claim 1 or 2 The culture method of the above-mentioned bacteria is characterized in that it is cultured using a solid medium prepared from a solid powder. 201036551 IV. Designation of representative drawings: (1) The representative representative of the case is: Figure (none). The symbol of the symbol of this representative figure is simple: 5. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: 201036551 正 ' I _ Invention patent said (the format, order of this manual, please do not change any more) ~~ --- ※Application number: please do not fill in. · ※Application date: % > ※ (four) Classification: _ handsome) I. Name of the invention: (Chinese / English) New Pseudomonas bacteria C 1 (2/1] (2(10)6.〇1> II. Chinese Abstract: 4 G〇〇()0]J The object of the present invention is to provide a method for controlling diseases as a cause of bees, animals, and vegetables, and a method for controlling diseases of bees, animals, and vegetables. The present invention provides a bacterium capable of inhibiting pathogenic bacteria, fungi and viruses, bacteria capable of infecting and proliferating bees, animals or plants, and inhibiting the active ingredients of the genus _, true 8 or the sense of the virus and increase the paste. As a new type of pseudomonas sputum bacteria, especially the pseudomonas von Vs MS 1 strain is ideal (trust number P-21673). III. English Abstract: 201036551 VI. INSTRUCTIONS: [Technical Fields of the Invention] The present invention relates to a novel pseudomonic bacteria, using the novel Pseudomonas, the stem bees, animals or A method for promoting growth of a plant, a disease prevention, and a method of cultivating the same. ', /, [Prior Art] Honey in the history of M is used as a view of the am and is used as a high-level hobby, not only in Japan, but also in Western Europe, Iss and the Chinese national circle. Honey is also popular. Food. The honeybee that produces the above honey in Japan has so far used Japanese honeybees. Since ancient times, this variety has been difficult to raise due to its habit of moving. At present, western honeybee is the main species in honey production. For western honey bees, there is a large loss due to the occurrence of several diseases. And the rot caused by the rot disease (European rot disease Meliss 〇c〇cus = national rot disease paembaeillus la ugly) A disease causes a large number of deaths. The above-mentioned diseases are sputum-borne diseases and the infective bee colonies must be incinerated in accordance with the law. In the countermeasures for the prevention of diseases, Pi ten" (preservative for preventing rot disease) containing antibiotics (miramidone) is used. However, there is an event that the above-mentioned medicine is caused by the formation of bees and honey, and there is damage to honey. The concern of pureness. The sputum caused by the fungus (asc〇sphaeraapis), it will be 3 to 5 3 201036551 ^ bee shoot high fiber, (four) _ community, snails on fungi and ... effect etc. Very few. In addition, in recent years, a virus has been produced in bees to cause the entire bee colony to disappear. The above situation is called a bee colony dissipating syndrome, and acute itching becomes a deadly virus. In response to the upturned poisoning disease, the county found an effective agent. In the social tendency to suppress the use of pharmaceuticals in food raw materials in recent years, it is necessary to establish a bee feeding method that does not use a drug because of the social improvement of the residual antibiotic material in honey. In addition, the diseases of livestock and vegetables are gradually expanding, and the use and patience of new drugs are formed _ or a new disease is repeatedly produced. For the reason for the occurrence and expansion of the above-mentioned diseases, the microbial imbalance of the environmental tissues such as the inside of the animal or the soil due to the use of a large amount of the drug can be cited. For example, in nature, 'sickness is inhibited by bacteria', but _ is reduced by antibiotics used at the time, and thus the virus liberated from the inhibition of _ is increased. The above phenomenon appears prominently in the livestock field where the vitamins (growth promotion) are fixed. Therefore, it is necessary to shift to a production method that does not depend on pharmaceuticals. In the present method, the disease is inhibited by the use of the useful bacteria which inhibit the proliferation of the pathogenic bacteria, the pathogenic fungi, the pathogenic virus, and the infection as the cause of the above-mentioned diseases. Therefore, the use of the above-mentioned useful bacteria can reduce and suppress the drug. Generally, the microorganism is cultured using a liquid medium to which a soluble nutrient is added to the liquid, and the ratio of the liquid to the solid medium is 100:1 or less in consideration of the sediment in the liquid. However, in the case of the above liquid culture, the amount of bacteria harvested per morning mi is low, and the maximum yield is 1〇9~1〇1〇bacteria fine cells/mi/mi, in the case of the cultivation of animals and plants. Under the circumstance, microorganisms must be cultured in large quantities Therefore, there is a need to significantly increase the method of transferring fine amounts. SUMMARY OF THE INVENTION The problem to be solved by the present invention is to provide a method for removing fine S, amount or remarks as a cause of bees, animals, and vegetables, and a method for promoting the growth of scale bees, animals, and vegetables. Surprisingly, the nuclear invention material, such as - containing Pseudo-caus (Fseud coffee as fons) MS-! strain (Wei number surface μ ·), belongs to the pseudo-cell + touch 'and has _ Bacterial secrets, the amount and the ability of the virus to infect and proliferate bees, animals or plants to solve the problem of the soil. The present invention contains the following invention. 〇 (1)—The species of Pseudomonas spp. (PSeUdQ_S f〇nS) has the ability to inhibit the infection and proliferation of bees, animals and plants. (2) The bacteria in (1) are Pseudomonas • Mars ms-m (commission number FEBl P-21673) or its variant. +(3) - Inhibition of infection and proliferation of bees, animals or plants by pathogenic bacteria The agent ' is a bacterium containing (1) or (2). (4) Phytopathogenic _ (4) Bee, animal or plant and inhibitor of proliferation ' is a bacterium containing (1) or (2). 5 201036551 (5 An inhibitor of infection and proliferation of bees, animals or plants of a pathogenic virus' contains bacteria of (1) or (2). (6) A growth promoter for bees or animals, containing (1) Or (2) a bacterium (7) - a growth promoter for plants, which is a bacterium containing (1) or (2). (8) a species used for bees, animals or plants because of bacteria, fungi or viruses The disease control agent is a bacterium containing 〇) or (2). (9) A feed and food or feed and food additive for bees or animals, which is a bacterium containing (1) or (2). (10) A fertilizer or fertilizer additive which is a bacterium containing (1) or (2). (1) A method for cultivating a bacterium of the species (1) or (2), which is obtained by culturing a culturing wire obtained by converting a solid powder. By using the novel Pseudomonas bacteria of the present invention, it is possible to prevent bees, animals, age = disease, sputum, salmonella, avian influenza virus: from the second prion disease, The spread of diseases, scabs, etc. In addition, Γ3: The solution is a bacterium, and the number of bees that can be obtained is greatly increased. The effect of the growth of the bee is: the effect of "Feng", while obtaining the healthy food of the animal and the vegetable body of the livestock, and the above results also show that It is used as an animal medicine or in addition to the above-mentioned bacteria using a solid medium. The following can be used to culture the above bacteria at a high concentration, and == is a bacterium [Embodiment] 201036551 The present invention will be described in more detail below.罝右 i Γ, 斤Pseudomonas genus is a Pseudomonas von von, and if:: p 'pathogenic bacteria, fungi and viruses inhibit the bees, animals or plants The ability to proliferate _ no special mosquitoes. For "animals" and "plants" It is guilty by the following. Examples of bacteria that correct the infection and proliferation of the filaments can be cited as rotten larvae ((4) chicks c〇_ or, Sal_lla infantls, Ralstonia solanacearum, sores ( Examples of fungi include the original fungus (Ascosphaera apis). Examples of viruses include avian influenza virus (10) N8), flounder anemia virus (IHNV), and rotavirus (R〇tavirus). However, it is not limited to this. The bacterium having the above-described ability is preferably Pseudomonas f〇ns MS-l strain (commission number M p_21673), but is not limited thereto, and for example, it can be used as the l6SrRNA. The partial arrangement of the genes is a bacterium of Pseudomonas vons, which is represented by the number 1 and a bacterium having the following bacteriological properties and classified into Pseudomonas vons. Shape: Bacillus; Length: 1~3 μm; Mobility: Yes; Cytogenesis: None; Gram stain: Negative; 0F test: - Fertility level After 1~2 days of culture, several groups of faces are formed, color: Thin brown; Gloss: No special; Surface: smooth; diffusive pigment: None; pH: 4. 0(—), 5. 0( + ), 7 201036551 5.6( + ), 8.6( + ), 10.0( + ) Temperature: medium temperature 7~45. 〇, this (1) ~ 4% ~; hydrogen sulfide: -; citric acid:, gland enzyme: one; oxidase:;: 68mol% reduction of nitric acid: one; denitrification reaction · - H -; nitrate utilization: one; Pigment production: - salt catalase: +; DNA G+C; the contents of the bacteria can be used in addition to the pathogenicity of the disease, the fungus and the virus to the infection and proliferation of bees, animals or plants, Better use of Pseudomonas • Von MS-! Dissimilarity, the so-called variable singularity, single code, code, ..., variants that are induced to undergo mutation. The mutation-inducing treatment can be obtained by arbitrarily mutating the original. Here, the term "variant" should be understood to mean, in a broad sense, for example, the term "except for the effect of money (four)", and also for those who have money and money when performing UV irradiation. Examples of the variation of the shot include: ethyl methyl sulphate, UV irradiation, N-methyl { _ hetero-N_ nitrosoimidodiamine, such as urinary uridine, but It can also be obtained by making any effective mutation of him. For the culture of the novel Pseudomonas bacteria of the present invention, a solid medium (large _ g, 雑 22Q () g, _ extract 4 g, steamed water 1000 ml and general nature can be used. The culture medium is not particularly limited. For example, a medium of 5 g of the culture medium, 3 g of the yeast extract, _2_Q. 2 g, distilled water ι ml ml pH 7. 2 to 7.4 can be prepared, and 121 t:, 15 It is used for heating and sterilization in minutes. 201036551 : 2: —===, _- For example, in Xiao Xian (4), τ彳/device 'and no special method. · Recognition, can be compiled into large-scale cultivation In the middle, the ferment tank can be used, and the solid medium θ soy powder prepared by the end of the "falling pot" can be used as the main type 1 powder, which can be used as a mother extract: =:=== Shuer Wei, at: 〇〇g solid powder is added with 2 〇 heart 40 妃, 60 hearts 80 rai as water, and the above-mentioned fine orchid culture, diced inoculation at 25 ° C, and cultured over 3 days, that is, 101 or more In the medium of water, the bacteria can be good, and the growth rate reaches 1Gl °~1() 12_cell/g. The ideal 疋 is relative to the solid The powder 1 bismuth is set to 140 ml. In addition, even if the inorganic particles (particle size (4) ") (4) g are added to the culture solution i L 2 singular genus bacteria increase the amount of bacteria to about = The number of oils is fine for 2w~5q g of the culture material. In the culture in which solids = or solid powder is added to the liquid medium, the invention is in contact with the nutrients of the magnetic properties, or so-called solids. The amount of bacteria collected is greatly increased, and the amount of bacteria harvested is more than that of the liquid to nourish the dilution of the sugar solution. The upper body culture is dared by water and J. It can be used as a bait for bees or animals, or as a fertilizer for planting 201036551, or as a dispersing agent for the reproductive environment of bees, animals or plants. In the _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Or culture in liquid medium _, unincorporated separation material ((4) squaring base ride "nutrient dilutions, concentrates, dried matter, frozen matter, etc.. «下=用:在可含上结束Cultivating _ nutrients and bacteria __ 5. _ 纽 纽 保存 r rtr / room 'by low temperature rotation, cold storage, can be effectively used for feeding, cultivation of bees, animals or plants., sugar === turn her bribe, sugar money After the sugar, I enter the custody of Jude. From the point of view of long-term preservation, the culture of the knot is kept under the age of the original, and it can be kept _ and long = the bee, the Lai, and the woman can use it effectively. End the culture 1 secret _ side彳 。 。 。 。 。 。 。 。 。 。 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因 因. (4):,::::, or its treatment, may be used in (4), for scientific purposes, for food use, for fertilizer use, for medical use, for veterinary use, for aquaculture, and for the purpose of making a woven carrier and making 201036551 Use: 'As a miscible substance, there may be mentioned two, fish meal, grain powder, seaweed powder, inorganic bacteria:: For the intended use, it may be any _. The right side is available. 2. For the use of bees. Ο Ο 白 白 Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο Ο And the honeybee or its young one ^ = bacteria, viruses (especially bacteria or true) bees (four) ^ method. There is no particular limitation on the effective amount of the new Pseudomonas bacteria, for example, pass to defeat Caga new pseudo-single cell __ (four) line feeding, and (d) group environment fog with a new type of Pseudomonas bacteria liquid (for example, the dilution of the culture) 4 etc. It is ideal to use Peptone, yeast extract, soy protein gland depending on the solid medium to culture the new Pseudomonas bacteria I hunting by water or sand recording to dilute the culture, and spray the bee to the bee or as a job = The larvae are ingested. It is advantageous to reduce the bee larvae by the administration of the novel Pseudomonas bacteria and to increase the survival rate of the young red, so that the number of bees can be greatly increased, which can be increased in the examples. Confirmed. 3. Uses for animals New fakes of the present invention The bacterium of the genus genus is useful in the prevention of diseases in animals (for example, diseases caused by 4 bacillus, rotavirus, and growth due to its growth). Validation and inhibition 201036551 That is, the present invention relates to a method comprising the step of administering an effective amount of a novel Pseudomonas bacterium to an animal, and preventing the animal, the fungus or the virus from being the cause of the disease. The term "animal" means livestock (for example, cattle, pigs), pets, poultry (such as poultry), or people. There is no limitation on the method of administering an effective amount of a novel Pseudomonas genus, for example, a method of cultivating livestock with a new type of pseudo-early cell bacteria, and a fertility environment for animals. The method of spraying the new pseudo single cell __ (4) is sprayed. The invention of the present invention actually confirms the suppression effect of the poultry of the cockroach disease and the improvement effect of the lower snail caused by the rotavirus caused by the serious growth insufficiency. For further steps, it can also be used in healthy foods for the human body. 4. Use for plants # The _ singular cell __ of the present invention is useful for preventing miscellaneous wealth (for example, ^Shenzhi Bing disease, genus-like disease). Here, the term "prevention" includes the age of treatment and inhibition. Namely, the present invention is directed to a method for treating a disease of a plant having an effective amount of a novel pseudo-singular genus for use in a plant, and a bacterium, a true function or a virus. The term "plant" refers to the selection of vegetables, shells, and shells. Specifically, it can be exemplified by cedar, pepper, broccoli, and taro. For the method of applying an effective amount of a new type of false singularity, the temple may, for example, be a method of mixing an effective amount of a novel Pseudomonas bacterium into the soil, and taking care of the secret body, The method of seed is specialized. In addition, the present invention relates to a method for applying an effective amount of (4) Pseudomonas sp. 12 201036551 to a crane and a difficult slave. The inventors of the present invention have confirmed that the above-mentioned growth promoting effect is favored in Fanyou, pepper, and broccoli. The method of application of the plant operates as described above. [Example 1] [Immediate rice cell letter] The isolation and homology of the Mas MS-1 strain] The strain was isolated from the freshwater environment and had the following symptoms. 2 · Shape bacillus, length: Bu 3 micron, motility: yes, formation of cells: no, Gram staining, negative, 0F test: a 3. Fertility level after 1 to 2 days of culture to form a group of coffee, color : Thin brown; Gloss: No special; Surface: Smooth; Diffuse pigment: None; pH ·· 4. 0(1), 5..),. ❹ 8. 6( + ), 9 ·〇( + ), 1〇 〇( + )·,, 田译 C + ), general. Medium temperature 7~ ant; _ ~ 4%, lemon gland: ~, oxidase: +, touch 4. Nitric acid reduction: -, denitrification Reaction: -,,::, hydrogen sulfide · · Acid: -, oxalate salt utilization: _, pigment production: -, medium: +, DNAG + C content · · 68 m〇i% 5.16SrRNA analysis After the nucleic acid was extracted using a Sepa Gene kit (manufactured by Sanko Pure Chemical Industries, Ltd.), the nucleic acid was recovered by the ethanol sink. The use of the _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The refined product is taken and the base is analyzed by applying the biological system 377. Thereafter, # is searched online by the database of the sheenbank. In the following, the column base of the 16S rRNA gene of the Pseudomonas ions MS-1 strain is shown as the corresponding DNA data (Table υ. According to the industrial classification review standard, the microbial industrial revision version (patent office)) The trait of this bacterium was discussed based on Baumann (lg84) and its taxonomic position. As a result, there was no recorded strain corresponding to this bacterium, so it was named pseud〇monas fons MS-1 strain. The inventor of the case The strain was named as Pseudomonas and Von MS_i, and was entrusted as the entrusted number FERM-21673 as the patent entity entrustment center of the Institute of Industrial Technology and Research, the independent administrative corporation on September 18, 2000. [Table 1] Pseudomonas • Von MS-1 strain 16S ribosomal RN gene, partial sequence length two 1531 score = 2773 bits (1399) E value = 0. 0 identifier = 1402/1403 (99%); citrate ribose Glycine amine synthase =0/1403 (0%) chain = positive / positive Pseudomonas fons MS-1 16S ribosomal DNA gene, partial sequence Length = 1531 Score = 2773 bits (1399), Expect ~ 0. 0 Identities - 1402/1403 (99%), Gaps = 0/140 3 (0¾) Strand = Plus / Plus 14 201036551 Query 9 TGAGTGGAGCTTGCTCCATGATTCAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCC 68 MIIMIIIIMIMIIIIIIIMIIIIMIIIIIimilllllllllMIIIIIIIM Sbjct 67 TGAGTGGAGCTTGCTCCATGATTCAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCC 12 6 Query 69 TGGTAGTGGGGGATAACGTTTCGAAAGGAACGCTAATACCGCATACGTCCTACGGGAGAA 128 INI IIIIIIIIIIIIIIIIJIIIIIII mill III limiMMIIIII MINIM Sbjct 127 TGGTAGTGGGGGATAACGTTTCGAAAGGAACGCTAATACCGCATACGTCCTACGGGAGAA 186 Query 129 AGTGGGGGATCTTCGGACCTCACGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGT 188 mmimmiimiiimmmiiiimimimMMiiimmii Sbjct 187 AGTGGGGGATCTTCGGACCTCACGCTATCAGATGAGCCTAGGTCGGATTAGCTAGTTGGT 246 Query 189 GGGGTAATGGCCCACCAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACAC 248 μ imi Mini i mil iimmi μ mi ill mi m mm mil mil 0 Sbjct 247 GGGGTAATGGCCCACCAAGGCGACGATCCGTAACTGGTCTGAGAGGATGATCAGTCACAC 306 Query 249 TGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATG 308 imMIMimimmiMIIIIMMIIIIIMIIMIMIIMMIIMMIM Sbjct 307 TGGAACTGAGACACGGTCCAGACTCCTACGGGAGGC AGCAGTGGGGAATATTGGACAATG 366 Query 309 GGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCAC 368 111111111111II111111! 1111111II1II1111111111111II1111II11111! 'Sbjct 367 GGCGAAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCnCGGAnGTAAAGCAC 426 Query 369 TTTAAGTTGGGAGGAAGGGCAGTAAGnAATACCTTGCTGTnTGACGTTACCAACAGAA 428 lllllllirilimil lllllllllllllllllllllllllllllllllllll! Ll] l Sbjct 427 TTTAAGTTGGGAGGAAGAGCAGTAAGnAATACCnGCTGTnTGACGTTACCAACAGAA 486 ^ Query 429 TAAGCACCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATC 488 IIIIIIIIIIIIIIIMIIIIIIIIIMIIMIIIIIIIIMIIIIIIIIMIMIMII Sbjct 487 TAAGCACCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATC 546 Query 489 GGAATTACTGGGCGTAAAGCGCGCGTAGGTGGHCAGCAAGTTGGATGTGAAAGCCCCGG 548 lllimillllllllllllllllMIMIIIIIIIIIIIMimilllllllllllll Sbjct 547 GGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTCAGCAAGTTGGATGTGAAAGCCCCGG 606 Query 549 GCTCAACCTGGGAATTGCATCCAAAACTACTGAGCTAGAGTACGGTAGAGGGTGGTGGAA 608 IIIIIIIIIIIIIIIIIIIIIIIIIIMIIIIIMIIIIIIIIIIIIIIIIIIIIIIIIIIII Sb jct 607 GCTCAACCTGGGAATTGCATCCAAAACTACTGAGCTAGAGTACGGTAGAGGGTGGTGGM 666 Query 609 TTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACC 668 mimimimimimiimmiimmmmmmmmm Sbjct 667 TTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACC 726 15 201036551 Query 669 ACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACC 728 iimmmiiMimimimiimiimMiimmmmmmi Sbjct 727 ACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACC 786 Query 729 CTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCGTTGGGATCCTTGAGATCTTAGTGG 788 llllimilMIIIMmiMIMIMIIIIIMMIMMlimmilMimi Sbjct 787 CTGGTAGTCCACGCCGTAAACGATGTCGACTAGCCGTTGGGATCCTTGAGATCTTAGTGG 846 Query 789 CGCAGCTAACGCGATAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAAT 848 IIMIIIIIMIIMIIIIIMIIIIIIMIIIIimimiimillMIMIMII Sbjct 847 CGCAGCTAACGCGATAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAAT 906 Query 849 GAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAG 908 imimimiimimmimimmmmmimmmmm Sbjct 907 GAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAAT TCGAAGCAACGCGAAG 966 Query 909 AACCTTACCTGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAA 968 lllllMMIIMMimiMMIIIMIMIMIIimmUIIMIIMMlM! Sbjct 967 MCCTTACCTGGCCTTGACATGCAGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAA 1026 Query 969 CTCTGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGnAAGTC 1028 Shu mm Shushu Shu m mm Shushu Shu mi mmmmmmm mn mum Sbjct 1027 CTCTGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGHAAGTC 1086 Query 1029 CCGTAACGAGCGCAACCCTTGTCCTTAGTTACCAGCACCTCGGGTGGGCACTCTAAGGAG 1088 iimmmmmmmmmmmmimmmmmmm Sbjct 1087 CCGTAACGAGCGCAACCCHGTCCTTAGTTACCAGCACCTCGGGTGGGCACTCTAAGGAG 1146 Query 1089 ACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGG 1148 iimmmmmmiiimmmmmmmmmmmmi Sbjct 1147 ACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGG 1206 Query 1149 CCAGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTTGCCAAGCCGCGAGGTGGAG 1208 ΙΙ!Ι!ΙΙΙ!ΙΙΙΙΙΙΙΙ!ΙΙΙΙΙΙ!Ι!ΙΜΙΜΙΙΙΙΜΜΙΙ!ΙΙΙΙΙΙΙΜΙΙ!ΙΙΙ! Sbjct 1207 CCAGGGCTACACACGTGCTACAATGGTCGGTAC AAAGGGTTGCCAAGCCGCGAGGTGGAG 1266 Query 1209 CTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTC 1268 Μ1ΙΙΙΙΙ! ΙΙ! ΙΙΙΙ! ΜΙΙΙΙΙ !!! ΜΜ !! Μ! Μ !! ΜΙ! Μ! ΙΙΙΙΜ !! 1! Ι! Ι Sbjct 1267 CTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTC 1326 Query 1269 GGAATCGCTAGTAATCGTGAATCAGAATGTCACGGTGAATACGTTCCCGGGCCTTGTAQ 1328 Sbjct 1327 GGAATCGCTAGTAATCGTGAATCAGAATGTCACGGTGAATACGTTCCCGGGCCTTGTACA 1386 16 201036551 Query 1329 CACCGCCCGTCACACCATGGGAGTGGGHGCTCCAGAAGTAGCTAGTCTMCCGCAAGGA 1388 ... Shu Shu jNNmmiiiMiimmiMimmmmmiiMimmi Sbjct 1387 CACCGCCCGTCACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGTCTAACCGCAAGGA 1446 Query 1389 GGACGGTTACCACGGAGTGATTC 1411 iimmiiiMiimiiiM Sbjct 1447 GGACGGTTACCACGGAGTGATTC 1469] Example 2 '[P. Adams • von MS-1 of the proliferation of pathogenic bacteria of strain suppression] billion follows It was confirmed that the above Pseudomonas Von MS-1 strain has an ability to inhibit the proliferation of pathogenic bacteria. Between the smear specimens of the two strains pre-coated on the soil lipid medium, 'Porterella infantileis, Ralst〇nia-Solanacea_, and cultured at 25 ° C for 10 days, after which they will be applied. The population size of the pathogenic bacteria between the specimens was compared with the population size in the case where only the pathogenic bacteria were cultured as a control region. As shown in Table 2, the proliferation of Salmonella g and Bacterial wilt was inhibited. The "inhibition rate" in Table 2 is expressed as a percentage of the size of the banner of the population of pathogens on the control zone medium, and the ratio of the size of the population of the test zone. In the same manner, the ability of the above-mentioned Pseudomonas mass MS] ^#t#f.J4^^(Melissococcus plutonius ^ Paenibacillus larvae) and the growth of Ascosphaera apis can be confirmed by the same method. [Table 2] Melissoccoccus plutonius inhibition rate (%) Paenibacillus larvae inhibition rate (%) —------ Ascosphaera apis inhibition rate (%) 17 201036551 --- Control area MS-1 strain 0 0 — 72 72 original bacteria Salmonella infant inhibition rate (%) Ralstonsia solanacearum inhibition rate (%) Control area 0 0 . MS-1 strain 73 75 [Example 3] [Pseudomonas ·; Mas MS-1 strain virus infection inhibition ability] In nature, there are a large number of bacteria that are poisonous or decompose the virus. In the case of natural wealth as above, if the side of the antibiotic side causes the virus-inhibiting bacteria (antiviral bacteria) to be eliminated. On the other hand, for most antibiotics, the virus remains thieves. As a result of the above, antiviral bacteria can be eliminated by using antibiotics, so that he is sufficiently infected and mutated for a lesser part.曰 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 The same scenario has occurred in the breeding of porpoise, poultry, cattle and fish. Therefore, in the present study, the virus inhibition ability in the useful bacterial MS-1 strain was measured. As described below, it was confirmed that the above-mentioned _Pseudomonas g·Creed _1 strain has the ability to inhibit the proliferation of pathogenic viruses. Furthermore, since it is not possible to cultivate a virus that is a cause of a disease of a bee, it is used as a virus of eapsid and her virus. It is a collection of 18 201036551, pottery and infectious hematopoietic necrosis virus (_ as a virus against the virus) More, it is caused by viruses and damage, and the eyes of the money are ruthless. Kaiqi 対j poison is more scaly. 'The cause of sputum is first, in the dog's kidney MDCK cells The clarification of the training of the station sr is based on the degree of sputum 叙 Ο 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 That is, the canine kidney MDCK cells were placed in a 1 m liquid medium (containing 1% bovine serum fraction, 〇.075··, (10) IU/ml penicillin, (10) material·bonded micro-sugar-HC1 (10).8)) In the culture system, Yudi was added, and avian influenza virus (10) 8) was added to culture the culture supernatant (〇.丨ml) of the 丨(6) and the Pseudomonas bacterium MS-1 strain. As a control experiment, only culture (10) (4) of avian influenza virus (10)/_ strain was added to the above culture system. Here, the culture supernatant of the above-mentioned pseudo single cell g is subjected to the above culture in the medium at 25 ° C for 3 days, guessing the bet (side (4) body position, and by 0.22 _ Cheng || In addition, as the avian influenza virus (BUD/H3N8 strain), the same culture medium and the kidney MDCK cells of the dog are used to proliferate the avian influenza virus (H3N8) by 0.45. The sputum obtained by filtering the filter was tested for Cpe after the above-mentioned cell culture supernatant was added, and the case where the cpe was low means that the proliferation of the virus was inhibited. The results are shown in Table 3, respectively. 19 201036551 In the case of the culture supernatant to which the pseudomonocyte g· _MS-1 strain is added, the proliferation of the avian influenza virus (BUD/H3M8 strain) is inhibited. Further, the part of the above method can be changed to confirm the pseudomonocyte In the above method, the strain of the bacterium, the amount of n, has the ability to replace the avian influenza virus c_:) necrosis virus (Li) ) to investigate, the result is 'pseudo cell __ US culture Liquid suppression fi] an infectious hematopoietic necrosis virus (Li) proliferation (Table 3). Furthermore, the ability of the virus appears as the culture cell infection price (TCIDm). [Table 3] Avian influenza virus (BUD/H3N8) Infectious hematopoietic necrosis virus control area MS-1 strain 105-8TCID5〇/25ul '>-- l〇57TCID5〇/25ul l〇19TCID5〇/25ul -- --- 101 5TCID5〇/25u1 [Example 4] ==® Attack test and pseudo-single cell _ fine _ strain disease prevention - in the occlusion system _ raise (four) set 3_ room, and 2 bees in each net A total of 3 bee colonies of one front group are reared. In the bee-boxes of the m _ group in the three bee colonies, the stalks were planted in the bee colony (the group 2, the other 2 bee colonies) (4) S-1 strains and the sucrose liquid as the honeybee of the bees. The remaining - one bee colony as an attack control group, did not vote for 20 201036551. MS-1 strain. During the MS-1 strain during the mid-course attack, the attack was observed within 5 weeks after the attack. The amount of feed intake, the number of larvae, the shape of the young bees and the status of the group (the tenth for the feed _ (four), the three groups _ (four). ·: the rot fruit sister is 'after the shot, lightly Temporary (10) bee view is in the 3 groups ο ^ Μ Μ Μ Μ Μ Μ Μ Μ Μ Μ Μ Μ 但 但 但 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏 魏The two bee shots, the county Wei rot 蛆 Newborn detached. The domain has been clarified as described above, and the useful bacteria in the bee colony can effectively prevent the disease of rot disease (Table 4). Table 4] Control group administration group I ------ Administration group π ----------- Administration method sugar liquid -_____ Spray MS-1 strain dilution for 5 weeks检测 Detecting the number 詈~~~~ ~- [Example 5] Test group [by Pseudomonas • Codes MS-1 strain for prevention of white disease] The bee colony was used for 8 beehives, and the dilution of the culture medium of the Ms-strain (four) was 1/H) times) sprayed into the bee's beehive, and observed the birth of the white ridge disease for 2 months, and the σ fruit is suppressed in the beehive of the sprayed plant. table 5). The value of the number of bee stings in Table 5 is expressed by the value obtained by equalizing the number of 4 boxes in each experimental zone by 21 201036551. [Table 5] Test group administration method I Control group No administration strain 8 I administration Group II spray MsS dilution solution [Example 6] [Pseudomonas • Mousse (10) strain of bee growth promotion effect] ^Shang Cong holds a high-inhibition _ strain, the material is plucked into the cake and the money is placed on the bee needle, and the effect on the whole honey is determined from _. The test time is only about 2 months. The test site is located in Sakae-cho, Miyazaki Prefecture, and Kobayashi City, Tongji County. For the injection and use of bacteria, the bees are tested daily. The result of the test and experiment is that there is a slight difference between the current purchase and the intake of water in the middle of the six mixed crops, _ lang _ ratio, all the strains cast the test scars, bees There was no problem with the health and the number of bees increased (Table 6). The number of bees in Table 6 is the average of each nest (box). Number of bees (after 5 weeks) 21 , 000 26 , 000 [Example 7] Number of bees (Week week) 16 , 000 22 201036551 [Determination and growth of diseases by Pseudomonas • Hoss Promote the effect of weight gain in poultry feeding trials] Each of the eight-day-old poultry (Nagoya Jiaohong) will be placed in a 6-day feeding trial of only 13 days. Addition of the secrets of each district: 5% (Table 7_丨), no added Jingbiao factory 2). As the basal feed, a mixture having no antibacterial property (Balance up 18, manufactured by Sakamoto Co., Ltd.) was used. Ο [Table 7-1] Breeding Nagoya Jiaohong, 8 days old, 1{), and (10) liquid c) Weight (g/only) 80 Feeding amount (g/day/day) after 10.0. π heart, m^ 9 10 11 12 13 14 15 16 92 98 104 109 115 120 125 130 10.6 11.2 11.8 12.3 12.9 13.1 13.8 14.0 ---- 1 [Table 7-2] Weight (g/only) The amount of exposure (g/only/day) Copper raised Nagoya cross-toe, 1 day old 8-day-old poultry, and Pseudomonas was added to the feed. 81 10.2 9 -- 10 11 12 13 14 15 — 1 16 92 97 102 107 112 118 121 126 10.6 -— 11.4 11.8 12.4 13.1 13.4 13.9 ———— 14.3 'The observation project of the chicks of the Mas MS-1 strain Results] [Phoenococcus is administered. 23 201036551 Appetite: Good fecal traits: solid (good) Vitality: good feather luster, etc.: good as the situation improves), atrophy, excess fat feed intake and weight: Table 7-1 and Table 2 (body weight necropsy (the presence or absence of pathological traits): no discoloration accumulation of the liver, etc., no abdominal swelling, no kidney stones, no abnormalities of the intestinal wall, L disease control effect] The small bird 100 is divided into 2 Group, for which group i is administered Pseudomonas • Maz MS1 strain 'and the other group is not cast. Also, Salmonella infantis is mixed with compound feed and administered to small birds. Later, in the small birds that were administered with the Pseudomonas®·_MS-1 strain, the number of small birds that occurred due to the Salmonella rod was greatly reduced (Table 8). [Table 8] ----- —~_ -- The number of episodes of Salmonella in MS-1 without added to the feed (all 5 〇) 43 The MS-1 strain was added to the feed '-6' [Example 8] 24 201036551 [The inhibition of the effect of the lower calf by the Pseudomonas von (4) strain], the presence of rotavirus in the sputum It is difficult to carry out the disease and it is difficult to carry out the food. This nuclear growth is significantly stagnant. Here, the milk is mixed with (10) ml and the single cell drop • Von Strain pulls the carcass culture solution and is administered to the calf. 30 heads ^ black hair and cows, about 30 days after birth) divided into 3 groups 'to vote for the first group of pseudo-bacteria · vons ship - 丨, pair. brother 2 group slaves and 2: owed, right The 3 groups did not vote for the slope. In the case of Herbs, Miscellaneous, and MS-Symbol, the symptoms of chin were significantly improved (Table 9). [Table 9] No Ms-i strain was added to the milk. The number of improved calves was 10 heads and 2 times. 〔 [Example 9] Saki 峨 峨 进 与 赖 将 将 假 = = = = = = = = = = = = = = = = = = = = Second, in the 1 group! / MS-1 mixed in the soil (1%), and the shame is applied, and the root ring of each seedling is not applied to the other group. And the growth of the pseudo-single/material measurement 3 (Table (8). The silk is, it can be seen that the growth of the tomato of the fermented Von MS-1 strain is promoted. 25 201036551 [Table 10] ··· --- Full length ~ ~—__ Spindle straight (white-soil without MS-1 strain 38 cm ------------- 43 cm - part of the soil surface 5 cm) MS-1 strain added to the soil 4. 0 mm -- 4. 5 mm For the 2 groups of the above-mentioned 3 weeks of material, the amount of bacteria (10) and Panacea (the cell / ml) 2 〇 ml is administered to the whole population. Root circle, and determine whether there is a disease. As a result, it was not clear that the occurrence of the disease was suppressed in the injection of the pseudomonas 囷• 斯s MS-1 strain (Table (1). [Table 11] Nine [Implementation 10] [Cultivation test of peppers of Pseudomonas and Von MS-1], 120 pepper seedlings (average main stem length 10.8 cm) in units of 6 〇 It is divided into 2 groups and planted. Next, Pseudomonas von V. MS-1 strain (Ba) was mixed in the soil, and 25 S was applied to the roots of the 60 seedlings of the first group of peppers, and 60 of the second group were No strain was applied. Further, the above tomato was measured to grow after 4 weeks (Table 12). As a result, it can be seen that the administration of Pseudomonas vons can promote the growth of pepper. [Table 12] 26 201036551 —-—- --- Length of main stem (average of 60 trees) MS-1 strain was not added to soil -~~~-- 19. 2 cm Soil MS-1 strain was added. 6 cm [Example 11] [Cultivation test of broccoli by Pseudomonas and Von MS-1] About 200 seeds of stalked cabbage are divided into 2 groups '1st group 1 〇〇 They were immersed in Pseudomonas Von MS-1 strain for 30 minutes in a state of small mesh. About 100 seeds of the second group were not impregnated. The seeds were placed on a water-containing sponge block and left for 2 weeks. As a result, it can be seen that the seeds of the broccoli from the sponge block are germinated and the stems are stretched, so that the length of the stem can be measured, and the seeds of the stems of the Streptomyces sp. MS-1 strain are germinated. The stem grows faster (Table 13) c [Table 13], _·· a - ..... - . -___ Stem length of broccoli (average of 100) No MS-1 strain is added 7. 8 Cm Add MS-1 strain--~~~------------------- 9. 1 cm --- -- ------ [Example 12 〕 [The prevention of sputum sputum sputum; | In the scorpion (especially the horse yam), the spread of scabs caused by actinomycetes (S. acidiscabies) nationwide. The above-mentioned diseases are caused by the surface infection of the hoe and cause voids inside, resulting in a decrease in the production amount of the sputum and a decrease in the sense of taste' and a decrease in the value of the commodity. For 27 201036551, the axis is less effective for this disease. Therefore, the seedlings of the horse and the potato were divided into two groups and the New Zealand was carried out, and the following tests were carried out. That is; =·Mas (10) strain (10)) mixed in the soil and applied 5G g to the seeds of the group of potatoes just before being applied to various prefixes, not to other plants. μ, ride on the bell and lose 3 (4) with or without disease (the table ... can be clearly expressed in the horse bells of the original strain of Pseudomonas), the generation of sores and diseases are inhibited. Furthermore, as a seed The number of gimmicks increased by more than (7) or more. Therefore, the number of jobs added from 1雠芋 is 1 group, and the rewards of (10) groups that are touched by lon species are related to the presence or absence of sore disease. 〕 -~· 1 Number of potatoes with scab disease / 100~^7 MS-1 strain not added to soil ----------------- 45 Add MS-1 to soil ----- 6 [Example 13] [Solid culture method of Pseudomonas and Von MS-1 strain] The big end 8GGg, rice bran 2G〇g, yeast extract 4g, distilled water 1 S1 body culture medium was prepared and heated for 12 minutes at 12 rc. In the above medium, (4) medium (soybean egg: mother extract 3 g, Wei Di potassium 〇 2 g, distilled water round ml, pH 7.2) was added. 50 ml of the culture medium of the MS-1 strain cultured in the form of ~7 yeast and mixed, and the result of the cultivation was carried out for 4 days, and the result was that the solid cultured (6) strain was grown in a light body. 28 201036551 Number of bacteria / g 10~100-fold (Table 15). [Table 15] Further, the above solid culture method can be applied to a common bacterium. For example, even if the soy peptone 5 g, the yeast extract 3 §, the sorghum sputum. 2 g., the distilled water 1000 w, the pH 7.2 2~7. 4 medium is added and 10 g is added thereto ( For example, 20 to 50 g) of the inorganic particles (granules of the outer side of the phytoplankton, the outer side of the phytoplankton), and the like, the granules are about 10 wm, and after the shaking, the Pseudomonas Von MS- The amount of bacteria in one strain also increased significantly. Similarly, in the case where particles of an inorganic substance such as zeolite were added, the amount of the cells was also increased in the same manner (Table 16). [Table 16] Culture method MS-1 number of bacteria (0曰) MS-1 bacteria number (after 5曰) Cultured by liquid medium 105 cells/ml 1〇9 to 101° cells/ml by Liquid medium + diatomaceous earth culture 105 cells / ml 101 ° ~ 1011 cells / ml [Simple description of the diagram] M. [Main component symbol description] μ,, 29 201036551 正 ' I _ invention patent said (the format of this manual, Order, please do not change any more~~1·--- ※Application number: please do not fill in. ·Application date: % >※(4) Classification: _handle) I. Name of invention: (Chinese / English New Pseudomonas bacteria C 1(2/1] (2(10)6.〇1> II. Chinese Abstract: 4 G〇〇()0]J The object of the present invention is to provide a method for controlling diseases as a cause of bees, animals, and vegetables, and a method for controlling diseases of bees, animals, and vegetables. The present invention provides a bacterium capable of inhibiting pathogenic bacteria, fungi and viruses, bacteria capable of infecting and proliferating bees, animals or plants, and inhibiting the active ingredients of the genus _, true 8 or the sense of the virus and increase the paste. As a new type of pseudomonas sputum bacteria, especially the pseudomonas von Vs MS 1 strain is ideal (trust number P-21673). III. English Abstracts: 201036551 VII. Application for Patent Scope 1. A bacterium 'belongs to Pseudomonas spp. · Pseudomonas fons, which can inhibit pathogenic bacteria, fungi and viruses against bees, animals or plants The ability to infect and proliferate. 2. The bacterium according to the first paragraph of the patent application is a pseudomonas, a von Sloan-1 (commission number FERM P-21673) or a variant thereof. 3. A pathogenic bacterium against bees, An inhibitor of infection or proliferation of an animal or plant, which comprises the bacterium according to claim 1 or 2. 4. A fungal fungus for the infection and proliferation of bees, animals or plants, such as an application The bacterium according to the invention of claim 1 or 2, wherein the bee, animal or plant dyeing and stimulating inhibitor of the virus is a bacterium according to claim 1 or 2. 6.------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- , animal or plant disease because of bacteria, true 9 defense = ' contains a fine t-thrust described in item (4) of the patent scope, material and petals ~ add-on 1 month to exhaust the bacteria described in item 1 or 2 The bacterial ingredient 4 additive is contained in the patent application scope 1 or I 30 201036551 11. The method for cultivating a bacterium according to the first or second aspect of the invention is characterized in that the culture is carried out using a solid medium prepared from a solid powder. 201036551 IV. Designation of representative drawings: (1) The representative representative of the case is: Figure (none) (b) A brief description of the symbol of the representative figure: Benefit 5: If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: