201004569 七、 指定代表囷： (一) 本案指定代表圖為：第（無）圖。 (二) 本代表圖之元件符號簡單說明： 八、 本案若有化學式時，請揭示最能顯示發明特徵的化學式： 九、 發明說明： 【發明所屬之技術領域】 優絡乳的加工方法 【先前技術】 在臺灣，肝癌一直在十大癌症排行前二名，而肝病也被視為「國 病」，長久以來國人死於肝癌、肝病的比例的確很高。肝臟是人體最大 的化學工廠《從營養素的加工、運送與儲存，到廢物、有害物質的處 理、解毒，都是經由肝臟。因此肝臟如果出了大問題，全身的新陳代 謝就會陷入混亂，嚴重影響健康。 在臨床醫學上’常以水飛薊(silymarin)用於治療慢性肝疾、肝硬 化、脂肪肝及改進肝功能，水飛薊可直接作用於肝細胞穩定肝細胞膜， 重建受損之肝細胞’從治療肝疾進而增強肝功能，使血中Gpt、GOT 恢復正常。 【發明内容】 本發明係以乳酸菌/_actojbac///ws ac/ctop/7//i；s (嗜酸乳桿菌）、201004569 VII. Designation of representatives: (1) The representative representative of the case is: (No). (2) A brief description of the symbol of the representative figure: 8. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: IX. Description of the invention: [Technical field of invention] Processing method of excellent colostrum [previous Technology] In Taiwan, liver cancer has been ranked in the top two cancer rankings, and liver disease is also regarded as a "national disease." The proportion of Chinese people who have died of liver cancer and liver disease has been high for a long time. The liver is the body's largest chemical factory. From the processing, transport and storage of nutrients, to the disposal of waste, hazardous materials, and detoxification, all through the liver. Therefore, if there is a big problem with the liver, the whole body's new generation will be in chaos and seriously affect health. In clinical medicine, 'silymarin is often used to treat chronic liver disease, liver cirrhosis, fatty liver and improve liver function. Milk thistle can directly act on hepatocytes to stabilize liver cell membrane and rebuild damaged liver cells' From the treatment of liver disease and thus enhance liver function, the blood Gpt, GOT returned to normal. SUMMARY OF THE INVENTION The present invention is based on lactic acid bacteria / _actojbac / / / ws ac / ctop / 7 / / i; s (Lactobacillus acidophilus),
Streptococcus thermophilus (味熱連珠菌）、Lactobacillus delbrueckii subsp· hw/sfa/ycws (保加利亞菌）進行優酪乳的發酵，並混合維生素 C(Vitamin C)、胺基丙酸(Alanine)、胺基乙磺酸(Taurine)及綠茶濃縮液 中的兒茶素(Catechins)，具有減輕四氯化碳所誘發之大鼠慢性肝炎的 功能’其效果優於一般肝病治療藥劑水飛薊(silymarin)。Streptococcus thermophilus, Lactobacillus delbrueckii subsp·hw/sfa/ycws (Bulgaria) for the fermentation of yogurt, mixed with Vitamin C, Alanine, Acesulfame Catechins in acid (Taurine) and green tea concentrates have the function of alleviating chronic hepatitis induced by carbon tetrachloride, which is superior to the general liver disease treatment agent silymarin.
機能優胳乳對四氯化碳誘發之大鼠慢性肝炎，可降低血清中GPT 201004569 H丁县值、可增加灰清白蛋白含量、可減輕脾臟腫大、可增加肝臟蛋 料二胁可Γ低肝臟纖維化程度。由這些結果明確顯示，機能優赂 乳了以減輕四氣化碳所誘發的大鼠慢性肝炎。 魯 月的，疋分為二階段進行’第—階段是脫脂奶粉與生乳均 二7」。。’峡馬溫瞬間賴’殺死牛射的污雜微錢，並降溫 OCII/ I ^加入乳酸菌種：1〇5〜1〇6 CFU/mL嗜酸乳桿菌、1〇6〜1〇7 心-二ΐ熱鏈球菌、1。6〜1。7咖趾保加利亞菌等進行發酵。乳酸 f::增殖到106〜1010 CFU/mL時，冷卻降溫停止乳酸菌的生 L 疋將〇.02〜謹％維生素C、0.1〜0_5%胺基丙酸、0.2〜0·6〇/〇 土乙.、Q.5〜1.2%果膠’依個人嗜好性可加人甜味劑、香 =容解後，再與400〜1600ppm兒茶素進行混合，經超高溫瞬間殺菌 ’死原料巾的3雜微生物。之後再將第二階段的組合物與第一階段 的牛奶進行混合，即可製成本專利之具有降低肝損傷 【實施方式】 本發明-種可降懈損叙祕恤合物及鄕法，是於第 將脫脂奶粉與生乳均自溶紐，贿高溫賴賴，觀 =性微6生物，並降溫至37〜机，再加人秦1Q6 CFU/mL嗜酸乳桿 菌、10〜10 CFU/mL嗜熱鍵球菌、· 107CFU/mL保加利亞菌等^Functionally good milk on CTC-induced chronic hepatitis in rats, can reduce serum GPT 201004569 H Ding County value, can increase ash albumin content, can reduce spleen enlargement, can increase liver egg material second threat can be low The degree of liver fibrosis. These results clearly show that the function is excellent in milk to alleviate chronic hepatitis in rats induced by carbon tetrachloride. Lu Yue's, 疋 is divided into two stages. The first stage is skim milk powder and raw milk. . 'Gorge Ma Wen Instant Lai' kills the bull's shot of the micro-money, and cools the OCII / I ^ added lactic acid bacteria: 1〇5~1〇6 CFU/mL Lactobacillus acidophilus, 1〇6~1〇7 heart - Fermentation of Streptococcus thermophilus, 1. 6~1. 7 toe Bulgarian bacteria, etc. When lactic acid f:: proliferates to 106~1010 CFU/mL, cooling and cooling stops the growth of lactic acid bacteria. 02.02~谨% vitamin C, 0.1~0_5% alanine propionate, 0.2~0·6〇/alumina B., Q.5~1.2% pectin 'According to personal preference, can add sweetener, fragrant = after disintegration, then mix with 400~1600ppm catechin, instant sterilization by ultra-high temperature 3 hybrid microorganisms. Then, the composition of the second stage is mixed with the milk of the first stage, and the patent can be made to reduce liver damage. [Embodiment] The present invention can reduce the damage and the sputum and the method. In the first skim milk powder and raw milk are self-dissolving New Zealand, bribe high temperature relies on, view = sex micro 6 organisms, and cool down to 37 ~ machine, plus human Qin 1Q6 CFU / mL Lactobacillus acidophilus, 10 ~ 10 CFU / mL Xenobiotics, · 107 CFU/mL Bulgarian bacteria, etc. ^
酸菌種進行發酵，至乳酸菌經發酵後，增殖到1〇6〜1〇1〇 CFU/mL 冷卻降溫停止乳酸g的生長。在另—階段是將QO2〜Q Q6%維生素C、 0」〜0.5%胺基丙酸、0.2〜〇.6%胺基乙績酸、Q 2%果膠、甜味 香料等均勻溶解後’再添加4〇CM600ppm的兒茶素進行混合 溫瞬間殺g後，再將第二階段的組合物與第—階段的乳‘發酵= 牛奶進行混合、充填、包裝，即可製成本專利之具有降 優 酪乳組合物。 谛又馒 本發明經如下的實驗，確實證明具有降低肝損傷的效果： 一'慢性動物肝損傷實驗 5 201004569 造成肝臟疾病之主要因素有病毒性、酒精性與化學性三大類，而在 老鼠動物模式之_切片中，與人體有—致之顏現象者為化學性肝 ，傷三所林評估方法以四氣化碳料大白鼠慢性肝損傷的實驗模 式，探討不同試驗樣品之處理，對於大白紐性肝損傷之影響。 四氯化碳餘賴油巾，g&製歧度2Q%的驗，每:域予量為 b μ 00g bodyweight。大氣口服機能優赂乳劑量’依據實驗動物 與人體表面積比等效劑量換算比率計算，雜w丨伽大鼠2㈤公克與 70 a斤人的表面積比值為0 018，因此成人每日飲用2㈤毫升，換算 為大鼠的劑量為200 mL X 0.018 = 3 6 mL/2〇〇 g㈣呢咖，即、18 ❹ 机/呦。本試驗使用的劑量分別為人體飲用量的一、二倍，即為200 ，升與4〇〇毫升。控制組械給予同體積的去離子水，對照—般治療 藥物水飛薊(silymarin)使用劑量為2〇〇 mg/kg。 ” 二、實驗結果 γρτ^Ι表肝炎實驗各組數據；A鼠投予四氣化碳八週之後， 值均顯著兩於控制組，機能優絡乳處理之大鼠對GPT、 古菩:細咖，水麵處理之大鼠對GPT與GOT㈣升高皆沒 百影響。 你予四氣化碳之後，血清白蛋白__值較正常控制組 乳處理之大鼠對血清白蛋白值有顯著增加作用，水飛莉 對四氣化碳引起的白蛋白下降沒有改善作用。 如表二的慢性肝炎實驗各組數據；大鼠投予The acid bacteria are fermented, and after the lactic acid bacteria are fermented, they are proliferated to 1〇6~1〇1〇 CFU/mL, and the growth of the lactic acid g is stopped by cooling and cooling. In another stage, QO2~Q Q6% vitamin C, 0"~0.5% alanine propionic acid, 0.2~〇.6% amino-based acid, Q 2% pectin, sweet flavor, etc. are uniformly dissolved. After adding 4 〇 CM600ppm of catechin to the mixed temperature and instant killing, the second stage composition is mixed, filled and packaged with the first stage milk 'fermentation=milk, and the patent can be made into a drop. Yogurt composition. The following experiments have indeed proved to have the effect of reducing liver damage: a 'chronic animal liver injury experiment 5 201004569 The main factors causing liver disease are viral, alcoholic and chemical, and in mouse animals In the pattern of slice, the phenomenon of the skin that is related to the human body is chemical liver, and the evaluation method of the three forests is based on the experimental model of chronic liver injury in four gasified carbon materials, and the treatment of different test samples is discussed. The effect of neonatal liver damage. Carbon tetrachloride residual oil wipes, g & 2Q% of the test, each: the amount of domain is b μ 00g bodyweight. Atmospheric oral function and excellent breast milk dose 'based on the ratio of experimental animal to human body surface area than the equivalent dose conversion ratio, the ratio of surface area of 2 (5) grams and 70 a kg of humans in the mixed w丨 大鼠 rats is 0 018, so adults drink 2 (5) ml per day, The dose converted to rats is 200 mL X 0.018 = 3 6 mL / 2 〇〇 g (4), ie, 18 ❹ machine / 呦. The dose used in this test is one or two times the amount of human body consumption, that is, 200 liters and 4 liters. The control group was given the same volume of deionized water, and the control treatment drug silymarin was used at a dose of 2 mg/kg. Second, the experimental results γρτ^ Ι 肝炎 肝炎 肝炎 肝炎 肝炎 肝炎 ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; In the coffee, the water-treated rats had no effect on the elevation of GPT and GOT (four). After you gave the four gasified carbon, the serum albumin __ value was significantly higher than that of the normal control group. Role, Shui Feili did not improve the albumin decline caused by four gasification carbon. Table 2 of the chronic hepatitis test data of each group; rat administration
肝臟及脾臟重量較控制組高，機能優絡乳組能減輕脾二重量H 四氣化碳組明顯高於正常控制組，機能優路乳組能減 水水麵的處理社鼠賴、軸重量及肝臟的含 四氣化韻發大_断炎，使賴的可雑蛋白f含量降低 有意義增加肝臟可溶性蛋白f含量，水_對肝賴可溶 白質含量的減少沒有影響。 6 201004569 慢性肝炎會引起肝臟纖維化’即結締組織增生。結締組織主要由膠 原蛋白構成，hydroxyproline是膠原蛋白特有的成分故測定 hydr〇xy_ne的量可以反應膠原蛋白的量，可用來表示纖維化的程 度。四氯化碳誘發慢性肝炎，其輯hydroxypr〇丨丨ne含量明顯辦加， 機能優酪乳能使hydroxyproline含量減少，顯示其可以減輕肝^維化 的作用’水飛薊對hydroxyproline含量的增加沒有影響。 總結以上實驗結果，此機能優酪乳配方，符合食品安全標準，且對 於肝臟有非常顯著的保護效果》 $ 表一、慢性肝炎實驗各組數據__ GPT (U / L) GOT (U / L) Albumin (g /dL) ❺ Control 60.6 ±2.9 7056 ± 3.3 3.56 ± 0.04 CCI4 1543.21137.7^ 1782.7 ^22.0^ 2.58 ± 0.06***' . CCI4+IX Recipe 1235.0 ± 116.8ab 1481.4 ± 156.2ab 2.75±0.06ab CCI4+2X Recipe 1043.9 ±86.1* 1267.3+114.7* 2.90±0.06b CCI4+Silymarin 1335.2 ±162.4ab 1747.7 ±209.0b 2.71±0.11*b 數字爲平均數土娜誤，表示與麵組差異檢定p<0.001者· 英文符號爲單因子變異數分析和Duncan’s多重比較結果. 1X Recipe爲大鼠投予機能優酪乳18 mL/ kg,換算成人每日飲用200毫升· 2X Recipe爲大鼠投予機能優酪乳36 mL / kg,換算成人每曰飲用400毫升·The weight of liver and spleen was higher than that of the control group. The function of the collaterals could reduce the weight of the spleen. The weight of the spleen was higher than that of the normal control group, and the weight of the functional group could reduce the water surface. The liver contains four gasification rhymes and large _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 6 201004569 Chronic hepatitis causes liver fibrosis, ie connective tissue hyperplasia. Connective tissue is mainly composed of collagen, and hydroxyproline is a characteristic component of collagen. Therefore, the amount of hydr〇xy_ne can be measured to reflect the amount of collagen, which can be used to indicate the degree of fibrosis. Carbon tetrachloride induces chronic hepatitis, and its content of hydroxypr〇丨丨ne is obviously increased. Functional yoghurt can reduce the content of hydroxyproline, indicating that it can alleviate the effect of liver vascularization. influences. Summarizing the above experimental results, this functional yogurt formula meets food safety standards and has a very significant protective effect on the liver. $ Table I, chronic hepatitis experiment data __ GPT (U / L) GOT (U / L ) Albumin (g /dL) ❺ Control 60.6 ±2.9 7056 ± 3.3 3.56 ± 0.04 CCI4 1543.21137.7^ 1782.7 ^22.0^ 2.58 ± 0.06***' . CCI4+IX Recipe 1235.0 ± 116.8ab 1481.4 ± 156.2ab 2.75±0.06 Ab CCI4+2X Recipe 1043.9 ±86.1* 1267.3+114.7* 2.90±0.06b CCI4+Silymarin 1335.2 ±162.4ab 1747.7 ±209.0b 2.71±0.11*b The number is the average number of soil errors, indicating the difference with the quilt test p<0.001 The English symbols are single factor analysis and Duncan's multiple comparison results. 1X Recipe is administered to rats with 18 kg/kg of functional yogurt, converted to 200 ml per day for adults. 2X Recipe is administered to rats. Milk 36 mL / kg, converted to 400 ml per adult
Silymarin冰飛薊)爲一般治療藥物,劑量爲200 mg / kg. 表二、慢性肝炎實驗各組數據Silymarin is a general therapeutic drug with a dose of 200 mg / kg. Table 2, data of each group of chronic hepatitis experiments
Spleen (g) Hepatic Water (g / g tisuue) Protein (mg / g tissue) Hydroxyproline (μρ / g tissue) Control 0.8 + 0.0 0.691 ± 0.004 150.3 ±1.7 791.7 ±23.8 ecu 2.0±0.1Wb 0.748 ± 0.004獅 122.1 ±2.5**^ 1387.9 ± 59.7***11 CCI4+1X Recipe 1.7±0_1ab 0.739 ± 0.006ab 128.2 ±4.8ab 1137.6 ±67.3" CCI4+2X Recipe 1.4±0.1* 0.726 ± 0.006* 139.6 ±4.0b 1035.9 ±53.8a CCU+Silymarin 1.9±0.1b 0.739 ± 0.004ab 126.1 ±5.2* 1244.7 ± 81.3b 數字爲平均數土標準誤；娜表示與對照組差異檢定Ρ<〇·〇〇1者· 英文符號爲單因子變異數分析和Duncan’s多重比較結果. 1X Recipe爲大鼠投予機能優酪乳18 mL / kg,換算成人每日飲用2〇〇毫升. 2X Recipe爲大鼠投予機能優酪乳36 mL / kg,換算成人每日飲用400毫升· Silymarin(水飛薊)爲一般治療藥物,劑量爲200 mg / kg. 【圖式簡單說明】 無 7Spleen (g) Hepatic Water (g / g tisuue) Protein (mg / g tissue) Hydroxyproline (μρ / g tissue) Control 0.8 + 0.0 0.691 ± 0.004 150.3 ±1.7 791.7 ±23.8 ecu 2.0±0.1Wb 0.748 ± 0.004 lion 122.1 ± 2.5**^ 1387.9 ± 59.7***11 CCI4+1X Recipe 1.7±0_1ab 0.739 ± 0.006ab 128.2 ±4.8ab 1137.6 ±67.3" CCI4+2X Recipe 1.4±0.1* 0.726 ± 0.006* 139.6 ±4.0b 1035.9 ±53.8 a CCU+Silymarin 1.9±0.1b 0.739 ± 0.004ab 126.1 ±5.2* 1244.7 ± 81.3b The number is the average number of soil standard errors; Na is the difference between the test and the control group Ρ<〇·〇〇1 · The English symbol is single factor variation Number analysis and Duncan's multiple comparison results. 1X Recipe is administered to rats with functional yogurt of 18 mL / kg, which is converted to 2 ml per day for adults. 2X Recipe is administered to rats with functional yogurt of 36 mL / kg. Convert 400 ml per day for adults · Silymarin (Silybum) is a general treatment with a dose of 200 mg / kg. [Simple description] None 7