TW200902033A - Neuroblast proliferate accelerant and nerve protuberance extend accelerant - Google Patents

Abstract

This invention provides a Neuroblast proliferate accelerant, the Neuroblast proliferate accelerant can activate the Neuroblast to proliferate and activate wherein a nerve protuberance to extend, even the Neuroblast is in healthy stage, the Neuroblast proliferate accelerant can still activate the Neuroblast to differentiate to a neuron, so as to prevent from the brain degeneration, and even to improve the function of the brain, the characteristic of the Neuroblast proliferate accelerant is to extract a phenylethanoid glycosides of Cistanche as a active principle, the phenylethanoid glycosides includes at least a echinacoside and an acteoside. The Neuroblast proliferate accelerant can apply to the drug, external use ointment, beverage and feed of the human kind and mammiferous animal.

Description

200902033 IX. Description of the Invention: [Technical Field] The present invention is a neuroblast (Neuroblast, neuroblast) proliferation promoter and a neurite stretcher, and the present invention can be widely applied to humans. In mammalian medicines, skin external preparations and foods. [Prior Art] According to the 'medical effect since ancient times, the fresh stems of the herbaceous plants are quite effective for diseases such as infertility, impotence, constipation (please refer to Patent Document 1), and in addition, the herb The fresh stems of plants are quite beneficial for the blood and kidneys. These perennial parasitic herbaceous plants are widely cultivated in northwestern China. In some places, they are called “desert ginseng”, while in the herb family, the parasitic plant is Cistanche tubulosa (Schrenk.). After the biggest. However, the author of this book is directed to the group (for the chemical and pharmacological activities such as plants - systematic research, the results found that the phenylethyl alcohol in the group is the main active ingredient (for example, j test non-paste) Read Bu 3), Ling ethanol brain compound can be used as an anesthetic agent, a metabolic enhancer, a memory improver and a libido promoter ^ caused by a school of acupuncture county ethanol brain (4) medical characteristics occur = Bu 1, the results show After the brain of the brain is restored to its other r-type compound, the original lesion may be b. In addition, please refer to Non-Patent Document 4, adding a 200902033 nerve bud cell to a TNFa to cause the neuroblast cell to undergo lesions, and then adding After the chlorophyll-like compound, the neurite of the neuroblastic cell can be restored to the original normal state. Patent Document 1: Japanese Patent Laid-Open Publication No. 2004-250449 Non-Patent Document 1: Sato T, et al. Yakugaku Zasshi , 1985, 105(12): 1131 Non-Patent Document 2: Jimenez C., et al. Nat. Prod· Rep., 1994, 11 (6): 591 Non-Patent Document 3: Cometa F., et al. Fitoterapia, 1993, 64 (3): 195 Non-Patent Document 4: Deng Min, et al. Pharmacoe Sin, 2004, 25 (10): 1276-1284 However, the above Patent Document 1 found that in the case of a brain function lesion, administration of a phenylethanoid glycoside compound can be made. Although the function of the brain is restored, there is no description in Patent Document 1 that the phenylethanoid compound can enhance the function of the brain before the lesion occurs in the brain. In addition, the above-mentioned Non-Patent Document 4 describes that the nerve bud cells are caused to have lesions. Thereafter, by giving the phenylethanoid compound, the neurites can be stretched to restore the function of the brain, but in Non-Patent Document 4, the following cases are not known: in the case where the nerve bud has no lesion, 'through Adding a phenylethyl alcohol compound can proliferate the nerve bud cells and stretch the neurites of the nerve bud cells. Thus, there are still many defects in the above-mentioned conventional methods, which is not a good method and needs to be improved. [Summary of the Invention] 200902033 has the disadvantages of the above-mentioned conventional use, therefore, the inventor has relied on The related experience of this subject has been developed and designed with the long-term efforts and research, and finally developed a "nerve cell proliferation promoter and neurite outgrowth agent" according to the present invention. People have discovered the fact that the addition of a stupid glucoside compound or a lindenyl plant extract containing phenylethanoid glycosides to neuroblasts (SK-N-SH) without lesions can promote neuroblasts. Proliferation, at the same time, can promote the neurite outgrowth of nerve bud cells, thereby completing the present invention, in other words, the present invention aims to provide a neuroblast cell proliferation promoting agent and a neurite outgrowth agent, even in the case where the neuroblast cells are not diseased, The neuroblast cell proliferation promoting agent and the neurite outgrowth agent can still promote the proliferation of the nerve bud cells, and can promote the stretching of the neurites of the nerve bud cells, thereby promoting the differentiation of the nerve bud cells into nerve cells, even in the nerve cells. In the case of degeneration, the bud cell proliferation promoter and nerve Since extension agent may effectively prevent reduction of the nerve cell, thereby effectively preventing the aging caused by the brain, while further improving brain function. In order to solve the above problems, the neuroblast cell growth factor of the first invention is characterized in that the neuroblast cell proliferation promoting agent has a phenethyl alcohol compound as an active ingredient. In addition, the nerve bud cell thinning promoting agent contains at least one of pinecone and chlorophyll bud as the compound. More importantly, the phenylethylidene compound contains pinecone and leaf-like stalks. . As a neuroblast growth promoting agent of the first invention, the 200902033 is characterized in that the neuroblast cell proliferation promoting agent is an extract of a group of Denko plants, and the extract contains a phenylethyl alcohol compound as an active ingredient. 'The phenylethanol turning compound contains at least one species of echinacea^ and a leaf-like extract, and more importantly, the extract contains both pinecone and eugenoid (4) as the aglycon Compound. Further, the neurite outgrowth agent of the third invention is characterized in that the neurite outstretching agent is an phenanthrene bud compound which contains an echinacea bottle and leaves At least one of its * and more importantly, the extract contains both echinacoside and flavonoids as the phenylethanoid glycoside compound. The neurite outgrowth agent contains at least one species of the genus Echinacea in the genus Echinacea, wherein the nerve = the stretching agent contains pine nuts (4) and the lycopene as the a phenylethyl alcohol compound, and the neurite stretcher as the fourth invention is characterized in that the scallops are extended as an active ingredient of the t plant, and the extract contains a phenylalanine compound. As an active ingredient, it is important that the extract contains at least one of Echinacea and C. sinensis as the phenylethanide, and more importantly, the extract towel contains pine. Both of them are popular as the phenethyl compound. The neuroblast growth promoting agent according to the first invention and the second invention of the above invention can promote the proliferation of the neuroblast cells and promote the transdifferentiation of the neuroblast cells even if the neuroblast cells do not have a lesion. The nerve cells can further improve the functions of the memory 200902033, the calculation of the force, and the fiber strength. In addition, even if the nerve cells are degenerated due to aging, the nerve cells are differentiated into nerve cells. It can maintain brain function's. Therefore, it can effectively prevent brain disorders such as aging or cognitive diseases. The neurite outgrowth of the third invention and the fourth invention, even in the case where the nerve bud cells do not occur, the neurite stretcher can promote the stretching of the neurites. Therefore, for the normal brain, since the nerve protrusions are stretched The agent can also construct a lot of synapses, so it can further improve the brain's function 'other-side' even if the nerve cells are degenerated due to the failure of the nerves, because the neurite outgrowth can be quickly constructed to compensate for the fineness of the nerve cells, so Switching to brain function can effectively prevent brain lesions such as brain aging or cognitive symptoms. Hereinafter, the present invention will be described in detail. In the present invention, a "nerve cell proliferation promoting agent and a neurite outgrowth agent (hereinafter also referred to as "nerve cell proliferation promoting") are characterized in that an alcohol-producing compound is an active ingredient. Further, in the present specification, bud cell proliferation promotes freshness, which means both "neuroblast cell proliferation promoting agent" and "neurite stretcher". Here, the "benzyl alcohol compound" refers to a compound represented by the following chemical formula (1):

The chemical formula (1) 200902033 is not particularly limited, and examples thereof include echinacoside, flavonoids, and 2'-acetylacteoside. Campneoside I, campneoside Π, Cistantubuloside A, Cistanche B., Cistanche B2, Cistanche, C. C. C. Crenatoside, decaffeeoylacteoside, isoacteoside, rhodiolaside, syringalide A 3'-ot-L-rhamnopyranoside and tubuloside A (Equivalent component) In addition, these compounds may be used alone or in combination of two or more. In addition, the nerve bud cell proliferation promoting agent contains at least one of echinacoside and cyanidin as a phenylethanoid glycoside, because these may have more significant neuroblast proliferation and nerve dogs. The stretching action and, more importantly, the neurite stretching agent contains at least echinacoside as an active ingredient because it can have a more excellent neurite stretching effect. The method for obtaining the stupid ethanol glycoside compound is not particularly limited, and it can be obtained by synthesis, or can be extracted from plants, and when extracted from plants, the plant as a raw material is not particularly limited, for example, : Listed as raw medicine Rehmannia glutinosa, Labiatae sylvestris, Piper pepper, Phyllostachys pubescens, and genus Herbaceous plants, etc., and one of the plants may be used alone to extract phenylethanoid glycosides, or may be used in combination Two or more plants are used to extract phenylethanoid glycosides, and among these plants, the best selection is from the genus Lepaceae, because in the genus 200902033, 'as a phenylethanol axis compound' except for echinacoside and In addition to the leaf asparagine, it also contains 2,2-ethyl ketones (2f-acetylacteoside) 'campneoside I > campneoside Π, cicadaubui 〇side a, tube flower meat Cistyl B1, Cistanche B. tubercosis, Cistanche tubulosa π, tube flower carmine glycoside C2, decaffeoyl acteoside (decaffeoylacteoside), heterologous leaf asparagine (isoacteoside) Salidroside (rhodioloside),

Syringalide A SH-rhamnopyranoside and tubeuloside A, etc., have more prominent neuroblast cell proliferation and neurite extension, and here, there is no particular limitation on the plant used as a raw material, for example: The whole parasitic plant Cistanche tubulosa, mConganche salsa) > mUAeginetia indica) > % (i ^{Boschniakia ros57ca), etc. 'but not limited to these plants, on the other hand, these Plants may be used alone or in combination of two or more. Among these plants, the best choice is to use the whole parasitic plant Cistanche tubulosa (Cistanche tubulosa). The components and contents of the phenylethanoid glycosides are listed below. The relationship between the chemical formulas (1). However, the structural formula of crenMoside is shown in the following chemical formula (2). The preparation contains substantially only a small amount or a small amount of other components than the echinacoside and the flavonoid in the above components.

Chemical formula (2) Crenatoside 200902033 Table 1 Main components of neuroblast cell proliferation promoter and neurite extensor: Component R1 R2 R3 R4 R5 R6 R7 2-Acety1acteos i de Ac Rha Cf HH OH OH Acteoside H Rha Cf HH OH OH Campneoside IH Rha Cf H 0Me(S/R) OH OH Campneoside II H Rha Cf H 0H(S/R) OH OH Cistantubuloside AH Rha Cf Glc HH OH 氺C i stantubu1os i des H Rha Cm/ Glc H OH OH B1/ B2 c-Cm *C i stantubu1os i des H Rha Cf Glc 0H(S/R) OH OH C1/C2 Decaff eoy1ac teos i de H Rha HHH OH OH Echinacoside H Rha Cf Glc H OH OH Isoacteoside H Rha H Cf H OH OH Rhodioloside HHHHHH OH (Salidroside) Syringalide A 3-αΊ H Rha Cf HHH OH -rhamnopyranos i de Tubuloside A Ac Rha Cf Glc H OH OH Crenatoside See above structural formula The compounds shown in Table 1 above can be passed by high performance liquid chromatography 200902033 (HPLC) confirmed that the conditions of high performance liquid chromatography were as follows, the stationary phase was C18 alkyl decane bonded tannin, and the mobile phase was acetonitrile-0.05 Μphosphoric acid. (Gradient elution 4: 96〇 15:85), flow rate of 1 ml / min, detection wavelength was 330 nm. The second neuroblast growth promoting agent of the present invention is characterized in that an extract extracted from a plant of the genus Leycote is used as an active ingredient. Here, the plant used as a raw material is not particularly limited, and for example, 'may be enumerated as the parasitic plant TubuJosa, salt C t (Cistanche saJsa), wild gas \ {Aeginetia indict), {Boschniakia rossica), etc., but not limited to these plants. In addition, these plants may be used alone or in combination of two or more. In addition, in these plants, 'the best choice is to use the full parasitic plant Cistanche tubulosa. Further, In the "extract", it is preferred to include a phenylethanoid glycoside compound. Here, the "phenylethanoid glycoside compound, 'f is not particularly limited'" may be exemplified by echinacoside or phylloside. - 醯, 甘, ... base class 2'-acetylacteoside, campneoside I, campneoside II, cistantubuloside A, tuberculosis B1, tube flower 蓰Rhizoma B, C. tuberculosis C1, Cistanche C. sinensis C2, crenatoside, decaffeoylacteoside, isoacteoside, rhodiolaside ),

Syringalide A 3'-a-L-rhamnopyrano side and tubeuloside A and other ingredients. At this time, the extract may contain only 14 of the above-mentioned components, and may also contain a mixture of two or more components. Among them, it is preferred to contain at least one of echinacoside and flavonoids. In the case where the oysters are used as a neurite outgrowth agent, the extract is more likely to contain at least the pineal chrysanthemum because it can have a superior neurite outgrowth. At this time, there is no limit to the content of the pine nut material, and the total quality of the extract of the plant (for example, 'whole parasitic plant tube flower (4) (仏(10) also (10))) is 1% by mass. The content of the fruit is ίο quality%~70% by mass, preferably 15% by mass to 50% by mass, more preferably 2% by mass%~4〇品步=, "~38_. In addition, the total quality of the class _^; = 口 有 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 % of quality, better choice is 7% by mass ~ Behind is further selected as 8 products «~1〇 quality%. There is no particular limitation on the extracted parts of the plant, _ ', 里, etc., as long as the desired effect can be achieved, that is, the selection of the genus from the stems of the plants, especially the fresh stems, is not The method for obtaining an extract from a plant of the genus Lepaceae, and the method of taking the method, etc., may be exemplified by a solvent extraction method, a supercritical extraction field, and a supercritical extraction method. When the extraction is carried out by using a solvent extraction method, the solvent used by the new agent is not (four) 200902033 There is no particular limitation on the above-mentioned polar solvent, and examples thereof include water, decyl alcohol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethyl-, propylene glycol, glycerin, acetic acid, and acetic acid. Ethyl ester, ether, etc. These solvents I may be used alone or in combination of two or more; preferably, they are subsequently subjected to dilution, agrotrophy, drying, and purification, because the treatment can be carried out by extracting a high concentration of stupid ethanol glycosides. Further, examples of the purification method include, for example, an activated carbon treatment, a resin adsorption treatment, an ion exchange resin, and a liquid-liquid countercurrent distribution. Specific examples of the preparation of the extract are detailed below, but the preparation method of the extract is not limited to the following examples. The method for producing the extract is composed of two stages of extraction and purification; in the first stage, the stem of the plant (for example, a parasitic plant & The fresh stems of 4 plants are selected to be sliced or pulverized into fine particles or powders, and then the sheet, or fine particles or powder is impregnated in water or a solvent or a mixed solution of a lower aliphatic alcohol such as methanol or ethanol. At this time, extraction was carried out at room temperature. Next, the mixed solution is filtered, and the filtrate is concentrated under reduced pressure or under vacuum to obtain an extract. In the second stage, after the extract is heated in water, the extract is transferred to the filled portion. Purification in the adsorption column of D-101 or AB-8 macroporous adsorption resin. At this time, use water, methanol, ethanol, a mixture of water and methanol, or a mixture of water and ethanol as a washing solvent. To flush the column. When rinsing, the rinsing solvent may be washed in a certain concentration of gluten solution, or the rinsing solvent may be washed according to a concentration gradient, and then the rinsing liquid is collected, and after concentration, the well-known drying method is used. Going through the line, hiding the money, taking the item 'The extract contains a stupid ethanol crane compound. The neuroblastic cell proliferation promoting agent and the like can also be used as various raw materials for drinking, for example, oil (salad oil), snacks (chewing gum biscuits, snacks, tablets, small). Desserts (such as c ramen, etc.), dairy products (milk, ice cream, etc.), condiments (weizeng, Daxin, etc.), soups, drinks (juice, coffee)

Ordinary it or healthy foods (tablets, capsules, etc.), nutritional supplements (nutritional beverages, etc.) can be appropriately equipped with the present invention, such as brown, black tea, tea, carbon, materials, etc. A neuroblastic cell proliferation agent, etc. According to this recording, various ingredients can be added to the towel, for example, glucose, fructose, sucrose, maltose, sorbitol, Stevioside, corn sugar, lactose can be used. , citric acid, tartaric acid, malic acid, amber, lactic acid, L-ascorbic acid, dh- = biotin E, sodium erythorbate, glycerol, propylene glycol, glycerol fatty acid 1, polyglycerol fatty acid I, miscellaneous fatty acid 酉Anthraquinone, propylene glycol fatty acid ester, gum arabic, carrageenan, casein, gelatin, pectin, agar, vitamin B, nicotinamide, calcium pantothenate, amino acids, calcium salts, pigments, spices In addition, in the present invention having the function of transferring health, that is, the nerve cell proliferation promoting agent, other substances such as antioxidant substances or health food raw materials may be added. An adjuvant, such as an antioxidant, reduced ascorbic acid (vitamin C), vitamin E, 17 200902033 reduced glutathione, tocotrienol, vitamin A derivative, lycopene, beta-corn yellow Quality, astaxanthin, zeaxanthin, fucoidan, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sesamin, lignin, catechin, isoflavones, chalcone, county Quality, flavonoids, aroma, isocoumarin, bilberry extract, bearberry bitter, tannin, flower, glycosides, apple polyphenols, grape seed extract, ellagic acid, kojic acid, sea buckthorn extract Health food raw materials, ν. (vitamin) A, VM, VB2, VB6, VB12, VC, VD, VE, VP, choline, nicotinamide, pantothenic acid, calcium folate, EPA, oligosaccharides, dietary fiber, horn _ , soybean _ fat, bovine acid, Dunali Ua, protein, octacosanol, dirty, egg yolk, phospholipid, linoleic acid, lactoferrin, town, remarks, chromium, strontium, unloading, ferrous Heme, oyster meat extract, acetaminophen, chitin oligosaccharide, proprotein, chondroitin, Yujin, licorice, stalk, cinnamon: hawthorn, ginger, ganoderma, mussel extract, turtle, licorice, stalk, cinnamon, hawthorn, ginger, ganoderma, plantain, gerbera, chamomile, dandelion, hibiscus Flowers, honey, seeking -k >, bee king, lime, lavender, rose hip, rosemary, sage, bifidobacteria, bacillus ^aecalis), cytosolic lactic acid g, wheat germ oil, _ Oil, purple sugar, soybean oil, medium chain fatty acid, Amicus, Ginkgo biloba, extract, turmeric, chondroitin, fresh germ extract, ganoderma lucidum, onion, DHA, EPA, DPA, sweet tea, Cordyceps sinensis, garlic , Bee Xuan, Papaya, Pu'er Tea, Propolis, Clairvoyance Tree, Ten, Royal Jelly, Mineral Leaf Palm Extract, Hyaluronic Acid, Collagen Protein, (D), Seal Oil, Shock Fish Cartilage, Glucosamine, (D) Lipid, Phospholipid 7. Mulberry leaves, soybean extract, Echinacea plant, Siberia 4 200902033 (work, / 々口年), barley extract, olive leaf, 撖 果实 fruit, Gymnema, big flower Ziwei, five layers Salacia, Garcinia, Garcinia, St. John's wort, jujube, ginseng, passionflower, green cabbage, placenta, alfalfa, grape seed, peanut skin, bilberry, black cohosh, water fly Quail, bay laurel, sage, rosemary, apocynum, balsamic vinegar, mouth 'one tea, Ma Jinn, safflower, flax, oolong tea, Oxytropis chinensis, ginseng, capsaicin, capsaicin Xylo-oligosaccharides, glucosamine, buckwheat, citrus, dietary fiber, protein, prunes, spirulina, barley green leaves, nucleic acids, yeast, shiitake mushrooms, plum meat, amino acids, deep sea shark extract, noni, Oyster meat, soft-shelled turtle, mushroom (シ10y (£二才y)' plantain, cherry (7 seven mouths today), pineapple, banana, peach, apricot, cantaloupe, strawberry, raspberry, orange, fucoidan , mulberry yellow, crane, chondroitin sulfate, zinc, iron, ceramide, si lk peptide, glycine, niacin, vine, ceramide, L-cysteine, L-meat, red grapes Leaves, cockroaches, quails, biotin, Centella asiatica, Lonicera japonica L., Pycnogenol® (PYCN〇GEIyj〇L ), butterbur, rhubarb, clove, rosemary, catechin, Pu'er tea, citric acid, brewer's yeast, ^ mouth - Bu, black soybean, ginger, medlar, natto, red peony, fertility Phenol, lactoferrin, cinnamon, buckwheat, cocoa powder, grapefruit seed extract, perilla seed extract, New Zealand seed extract, evening primrose extract, extract, α-lipoic acid, GAM, green coffee bean extract , butterbur extract, _ peach seed extract, Wenzhou orange extract, ginger extract and astaxanthin. As a specific manufacturing method, β can be used as a method of spray or cold drying, and can directly use the present invention as a cell proliferation promoter. 19 200902033 (If it is an extract, the extract and the dextrin ) made into powder, :: product ί = externally and thus contain them according to the needs of the 'only need to light the cells of the invention to promote the fresh scales (four) and the arsenic mixture ~ 'clothed into powder or granular And then added to the solid food ▲ In the case of the extract, 'the extract can be directly added to the drink '', the extract can also be dispersed and dissolved in, for example, water, ethanol, glycerin sister towel, and then added (4) In the beverage. The neuronal fine hybrid supplement of the present invention may also include a medicinal product and a "item" tree, and may be added to the nerve bud cells of the present invention to increase the quality of the product, and the drug may be used for humans. Class: substance: as a nerve bud cell which can be combined with the present invention (曰 glucose ': 丨: σ preparation raw materials, for example, 'except excipients = fiber::::, kaolin powder, etc.), dry agent (distilled water , physiological saline, ethanol: lings powder solution, gelatin solution, methyl cellulose, potassium acid, cesium, etc.), disintegrating agent (sodium alginate, _, sodium bicarbonate, two '', sodium lauryl sulfate, single hard Fatty acid glycerin vinegar, house powder, lactose, ==, gelatin, ethanol, etc.), disintegration inhibitor (white sugar, hard stone n, etc.), absorption enhancer (season money domain, laurel ^ 酉夂 (4)), adsorption Agent (glycerin, house powder, lactose, kaolin, soap diol = 3) and lubricant (refined talcum powder, stearate, polyethyl 20 200902033 as a method for administering the god, bud cell proliferation promoting agent of the present invention , / - In general, tablets, pills, soft capsules, hard capsule microgranules, powders can be used Oral administration in the form of granules or the like, and in addition, as a preparation, the water-soluble preparation may be administered orally or may be administered orally. When administered as a non-oral preparation, the nerve bud of the present invention may be finely divided. The proliferative enhancer or the like is dispersed in a suitable soluble reagent such as ethanol or water, and then used in a dosage form such as a cataplasm, a lotion, an ointment, a nail, or an emulsion, and the neuroblastic cell of the present invention. The water-soluble preparation for promoting proliferation can be directly used, or the water-soluble injection can be added to a dispersing agent, a suspension agent, a stabilizer, etc., and woven with a cloth, a lotion, a soft agent, a lyrium, a milk, etc. The dosage form can be used according to the administration method, the condition, the age of the patient, etc., but generally, the active ingredient which can be administered by an adult in an i day is 5 mg to 400 mg, and the child is administered according to i day. The active ingredient is administered in a dose of about 0.5 mg to 200 mg. When the neuroblastic cell proliferation promoting agent or the like of the present invention is used as a medicine, the ratio of the addition can be appropriately changed depending on the dosage form, and generally, it is administered orally. Dry film suction When administered, the addition ratio of the neuroblast growth promoter or the like may be about 〇1 % 1% by weight to 1% by weight, and when not administered orally, the addition ratio of the nerve bud cell proliferation promoting agent or the like may be 0.01. The weight is about -20% by weight. In addition, since the dose is adjusted according to various conditions, sometimes even if the dose is less than the above-mentioned dose range, sometimes the dose needs to exceed the above dose range. The pharmaceutical composition may contain, in addition to the above-mentioned neuroblast cell proliferation promoting agent, the other compounds which are commonly used in the field of medicine, and may be contained in a compound suitable for oral administration. A compound which is required, and examples of the compound include lactose-winning, hydroxypropylcellulose, kaolin, talc, and calcium carbonate. In addition, the mammalian animal feed may also contain the neuroblast-promoting solution of the present invention, and the nerve bud-proliferation promoting solution of the present invention may be included in the animal feed in the same manner as the above-mentioned drinking time product. The animal of the animal feed that can be used is not particularly limited. For example, the feed can be used for livestock animals such as cattle and pigs, dogs, babies, hamsters, and the like, and can be used to make _ powder and gamma, at this time, as: face: There are wheat flour, rice flour, rye flour, oat flour, glutinous rice: gossip gluten corn flour and large ugly powder, etc. 'These flours can also be used in two kinds of flour to provide pets with carbohydrates; f, Lu Yangsu In the flour, the best choice is to use wheat flour, the town 2 can be used alone or in combination to make a strong powder, medium strength powder °, = ^ 'the other wheat flour can also be combined with other flour, another: two = animal feed elasticity after heat treatment, can also be used in combination with small 2, small 4, soy protein, etc., and the reticular structure of the skin in 3 of wheat will form a [structural structure after heating treatment. For improvement Taste. / For the present invention, cut _ _ _ with chicken, pork, beef, single inside... followed by 疋 can make horse meat, etc., from the taste: Kao Guang most: two: meat, rabbit meat, turkey and used ancient, earthen ancient test The use of chicken meat can be obtained by using the animal meat and dissecting the animal meat, and the production of the intermediate water knife product or the low water state is caused by fat oxidation, so the consumption = 22 200902033 A small amount of fat or fat that has been removed. In addition, the consumption of meat in the feed not only provides excellent animal protein, but also improves the pet's taste.

Although the pet food can be formulated using various square yarns, a preferred method is as follows: preparing a raw material mixture (hereinafter referred to as "dough,") which is mixed with flour (preferably wheat flour) and edible meat, to be formed. After looking for heat treatment, there is no _ mosquito to the flour and meat composition of the raw _ towel, and the silk is about 5 (four)%, preferably about 10% to 50%, 5%~_ The left and right, preferably selected from 2G% to 5 (10) of the edible meat and the necessary amount of moisture, and the amount of the Aw modifier added is about 5% to 30% in the case of the Aw adjustment. The preferred choice is about 1% to about 2%, and the amount of water can be appropriately adjusted according to the mixing stool of the dough, the degree of forming, and the amount of flour, meat, Aw conditioner, etc. The preparation method is not particularly limited. Preferably, the meat is first made into a meat by a chopper, a chopper, etc., in order to sufficiently contain bubbles in the meat to be made into meat. It is best to cut the meat carefully and then make it Add the flour, water and the Aw conditioner according to the edible meat, and mix it to make it contain bubbles, so as to prepare the dough containing the bubbles, in which the dough leaves can be added. In the case where a foaming agent is used as the flour in particular, other than wheat flour, it is preferred to use a foaming agent 'by adding a foaming agent' to uniformly contain fine bubbles in the dough. As the foaming agent, various foaming agents can be used, but from the viewpoint of stability of the bubble, etc., it is preferred to use a soy protein 23 200902033 type foaming agent or an enzymatically decomposed soy protein type foaming agent. The heat is applied to the appropriate shape, for example, when the dough is formed, according to the easiness of the pet at the time and the ease of operation of the breeder, etc. a sheet, a rod, a disc, a ring, a heart, etc., in addition, the dough prepared from the sage component can be dyed with different pigments, or added with vegetables or fruits, etc. In order to produce a variety of raw materials with the same appearance, and then combine the raw and thin into a multi-layered or concentric _ shape, into the shouting type, the force of the dough into the pass: there is no special limitation on the thermal means, for example, The heating means can be combined with oven heating and microwave freshening. These riches are common to the heat treatment, and after the added feed, the moisture content is generally about the victory, after the heat treatment. After that, since the water evaporates and the gas swells, that is, the dough is puffed, and since the water is evaporated in a short time, a improves the preservability. In addition, the wheat flour is used as the flour: Heat treatment of the reticular knot of the dough contained in the wheat flour to improve the taste. And 'in the case of heating the secret box, there is the advantage of producing a unique color (fox color) or aroma in the feed' On the other hand, in the case of microwave heating, since the inside of the pellet starts to be heated, the dough can be uniformly expanded and the feed containing uniform bubbles can be obtained. In the case of _ n, it is preferred that the Aw in the obtained feed is adjusted within a range of two 〇. As described above, the preservation of the feed can be remarkably improved by adjusting Aw within this range. 24 200902033 The scent of Na, which is prepared by the above-mentioned green, is a kind of bread-shaped feed. 'Because the feed has a sense of sensation and appropriate softness, it is suitable for the fangs, old dogs and other teeth. Feeds, snacks, etc. of accompanying animals. The animal feed described above is contained in a packaging container in an appropriate amount and sealed to form a product. As the packaging container, it is preferred to use an oxygen-impermeable packaging material. The shape of the baggage may be a vacuum package or a package filled with activated carbon, but

It is preferred to package with both an oxygen scavenger (e.g., a 2 Μ TM, etc.) and activated carbon. In the form of packaging, it can prevent the deterioration of quality and microbial growth from Wei (4) during storage. ” The proliferation of nerve cells in the present invention can also be used as a skin agent (including cosmetics, pharmaceuticals, and (4) Items), it is expected that the external skin of the skin has a neurite outgrowth effect. In addition, the skin may be externally human, or (4) other than the human beings, other feedings and scorpions as the nerve buds of the present invention can be compatible. The form of the skin external preparation for promoting cell proliferation 1 may, for example, be an emulsion, a fat, a facial cleanser, a bathing agent, a skin cream, a lotion, a lotion, a toilet water, a cream, a lotion, a strip of oil, and an anti-aging agent. _b makeup water, powder =, foundation, perfume, mask, nail paste, finger oil (_Pak = nail polish, eyebrow, rouge, eye cream, eye shadow, mascara, eye red, lip f, A shampoo, a conditioner, a hair dye, a dispersion, and a second, and a form of a pharmaceutical product or a pharmaceutical product which can be used together with the nerve bud cell entrainment agent of the present invention, etc. , emulsion, external liquid, etc. 25 200902033 In addition to the above-mentioned form of skin (4), in addition to the proliferation of the nerve cells of the present invention, in the range of not (four) neurite outgrowth, the following substances may be added, and the substance may be added. Ingredients for skin external preparations such as makeup buttons, medicines, etc., including oil components, higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, multi-parts, sugars, polymers, physiological activities "Material" mixture, k oxidizing agent, perfume, _agent, etc. Although the following is a list of some of the fresh substances, the present invention is not limited to these examples. U) Examples of oil components The oil components of esters are exemplified by: -ethylhexanoate, hexadecyl 2-ethylhexanoate, isopropyl vinegar, diced acid, isopropyl citrate, stearic acid, brown eucalyptus S, different hard ore, different 16 _, hard lindane, peas, butyl ester, linoleic acid, isopropyl linoleate, oleic acid, and myristic acid , 岐 grade different eighteen _ purpose, palmitic acid isostearyl vinegar, myristate octyl ten _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ethylhexanoic acid trimethylol propyl ester, triisostearic acid di & methyl propyl glycerol, tetra-2-ethylhexanoic acid sterol vinegar, caprylic acid cetyl alcohol, lauric acid money, laurel酸 己 、, myristic acid 癸 turtle, meat ugly acid 岐 «_ purpose, meat domain needle six _ purpose, stearic acid stearyl ester, oleic acid decyl ester, ricinoleic acid cetyl ester, laurel Acid isostearyl alcohol ester, isotridecyl myristate, isohexadecanol myristate, isostearyl myristate, isohexadecanol palmitate, isostearyl palmitate, Octyl stearate, isohexadecanol stearate, isodecyl oleate, 9 octyldodecanol oleate, octyldodecanol linoleate, isopropyl isostearate, 26 200902033 2 - Ethyl hexadecanol hexadecanol ester, octadecyl ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol diacetate, Di(octyl decanoic acid) propylene glycol ester, dioctanoic acid propylene glycol cool, two Neopentyl glycolate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tris(undecanoate), glyceryl triisopalmitate, glyceryl triisostearate, octyl 12 pivalate Alcohol ester, isostearyl octanoate, octyl isophthalate, hexyl dodecyl neodecanoate, octyl dodecyl neodecanoate, isohexadecyl isostearate, isostearic acid Octadecanol ester, octyl undecyl isostearate, polyglycerol oleate, polyglyceryl isostearic acid vinegar, dipropyl carbamate, dialkyl (C12-C18) carbonate, citric acid Hexaol ester, triisodecyl citrate, triisooctyl citrate, lactic acid lauryl, crotonyl lactate, cetyl lactate, octyl lauryl lactate, triethyl citrate , Ethyl citrate triethyl citrate, Ethyl citrate tributyl sulfonate, Trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, succinic acid 2 -ethylhexyl ester, diisobutyl adipate, diisopropyl phthalate, dioctyl sebacate, cholesteryl stearate, isostearyl cholesteryl hard Acid bile to the purpose, oleic acid bile glycol vinegar, dihydrocholesterol oleate, isostearic acid plant transesterification, oleic acid plant sterol, 12-stearyl sulfhydryl sulphate Hexaol, 12-stearyl sulphate, stearic acid, stearyl sulphate, 12-stearyl sulphate, succinic acid, stearic acid, etc. (4) Examples of oil components are m-thin, liquid-stone, alpha-dilute hydrocarbon oligomers, iso-money, Baidi-san, stone sputum, liquid stellite, polybutylene, microcrystalline, and petrolatum. Examples of animal and vegetable oils and their hardened oils, as well as natural ants are: tallow, hardened tallow, lard, hardened lard, horse oil, hardened horse oil, simmered oil, and only - (thorny 27 200902033 hardened oil; avocado oil , almond oil, heart and other animal oils and seed oil, almond oil, Mu Liguang scented oil can be removed, "peach rice germ oil, black rice oil, safflower oil, butter, oil 'tea seed oil, camellia oil, jade =: : Month: Cao Cai Xuan oil, palm kernel oil, hardened palm kernel oil, flower oil, castor oil, hardened castor oil, sunflower glutinous oil, hardened jojoba oil, _ nut two = Xuan:, Hoho seeds Oil, butterfly _, (four), hardened coconut = and = hardened oil; bee sting, high acid price bee, lanolin, reduced sheep = 2 = hair fat, liquid lanolin, Brazilian brown side, brown coal butterfly and so on. Examples of the oil components of the lactane include: dimethyl poly, methylcyclo-poly-form, octamethyl-poly-n-== 夕 m m-cut, formazan modified organic poly-f-methyl = Zhuang, - Wei Weizhuo. Methyl rights domain secret =, silk secret machinery and wealth, material miscellaneous sand oxy-combustion: wind-based modified stone night oil, amino modified organic polymerization Dioxilconol, Dimethlconol, Lixi Kejiao, Bingfuji, Trimethylhydrazine, Oxygen, and Shixi_RTV, etc. Examples of fluorine-based oils ^. Gas-polymerized I-fluorine-changing machine, Wei Weixuan, fluorinated shot, fluorinated carbon, ^-alcohol, rat-derived base, poly-based mesomorphic group, modified organopolyoxane, etc. ^Example of higher alcohol There are: lauryl alcohol, myristyl alcohol, wax alcohol, octadecyl alcohol, isooctadecanol, oleyl alcohol, behenyl alcohol, 2_ethyl 28 200902033 hexanol, hexadecanol, octyl Twelve alcohols, etc. (3) Examples of fatty acids are: caprylic acid, capric acid, decenoic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid , linoleic acid, linolenic acid, arachidic acid, peanut tetrazoic acid, dodecanoic acid, sinapic acid, 2-ethylhexanoic acid, etc. (4) Examples of ultraviolet absorbers include · amino benzoic acid, amino-amino benzoin Touch, occupational rest, Weiethyl dihydrogen, brewing, gas-based rest, Wei Gan, p-amino, benzoic acid, cis-amino benzoic acid, sylvestris Salicylic acid 2 diol vinegar, salicylic acid sulphate, salicylic acid triethanolamine, salicylic acid benzene vinegar, salicylic acid butyl sulphate, salicylic acid (four) purpose, water riding with Meng § purpose, cinnamic acid festival Vinegar, p-methoxycinnamic acid vinegar, p-methoxycinnamic acid 2-ethylhexyl ester, di-p-methoxycinnamic acid mono-2-ethylhexanoate, isopropyl p-methoxycinnamate, P-methoxycinnamic acid diethanolamine, diisopropyl. Diisopropyl cinnamic acid ester mixture, urinary acid, ethyl urate, hydroxymethoxybenzyl, methoxymethoxybenzoic acid And its salt, dimethoxymethyl benzoyl, sodium dimethyl benzophenone sulfonate, dibenzobenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctyloxybenzophenone , tetrahydroxybenzophenone, butyl methoxy dibenzoylmethane, 2,4,6-triphenylamine p-(2-ethylhexyl-1-carboxy)_ι, 3, 5-triazine, 2- (2-hydroxy-5methylphenyl)benzotriazole, o-aminobenzoic acid methyl ester, 2-amino-3'3-diphenylacrylic acid 2-ethylhexyl ester, phenyl benzimidazole Sulfuric acid, 3-(4-mercaptobenzylidene) camphor, isopropyldiphenylmethylmethane, 4-(3'4-dimethoxyphenylmethylene)_2, 5-dioxoimidazolidinylpropan-2-ethylhexyl ester, and the like, or a polymer derivative of these materials or Shi Xi 29 200902033 Derivatives, etc. (5) Examples of powders and pigments include red 1〇4, red 2〇ι, yellow 4, and blue! No., black 4〇1 and other pigments; yellow No. 4 AL sink; temple color, yellow 2_β Α sink; temple color and other sinking pigment; nylon powder, silk powder, amino bismuth citrate powder, Teflon (Registrar powder, Shi Xi _ powder, polymethyl methacrylate powder _ powder, cellulose powder, lake:, = spherical elastomer powder, polyethylene powder and other polymers; yellow iron oxide, color emulsified iron, black oxidation Iron, chrome oxide, carbon black, ultramarine blue, dark blue and other colored pigments; zinc oxide, titanium oxide, oxidation _ stone powder, mica, self-dance, pure earth pigment; slip tape mother (four) read..., slab-like enamel and other body pigments ; metal salts such as bismuth citrate, paste, magnesium carbonate, _, 夕酉义镇; Shi Xi, Oxidation and other inorganic powders such as magnesium stearate, palm strontium zinc, zinc sulphate day zinc sulphate, ten-dilute acid Words such as metal fertilizers; swelling == scales. The shape of these powders (spherical, rod-shaped, needle-like, plate-like: Γ and spindle-like) and particle size are not particularly limited. ί, frequency grease treatment, Draping treatment method, rational method, _ mixture treatment method, oil treatment method, Ν-treatment method, metal fertilizer method, 'i treatment method, no Compound processing method, a surface treatment and the like. Cherry method nettle) of these powders advanced pre

W and ("Examples of lingual agents" ionic surfactants may be exemplified by fatty acid fertilizers, 苴AA ot-fe sulfonates, alkyl sulfonates, alkane 30 200902033 propyl propyl acid salts, Alkyl naphthate, alkyl sulfate, p〇E alkyl ether sulfate, alkyl amino sulfate, alkyl phosphate, p〇E alkyl phosphate, alkyl amino phosphate, alkyl alkane Taurate, N-mercaptoyl acid salt, oxime alkyl ether carboxylate, alkyl succinate, sodium alkyl sulfoacetate, thiolated hydrolyzed collagen peptide salt, perfluoroalkane Phosphate esters, etc. The cationic surfactants may be exemplified by alkyl tridecyl chloride, stearyl trimethyl chloride, stearyl trimethyl ammonium bromide, and octadecyl fluorenyl. Hexadecamethyltrimethylammonium hydride, distearyl dimethylammonium chloride, stearyl dimethyl benzyl ammonium chloride, docosalyltrimethylammonium bromide, Gasification of neostigmine, behenic acid aminopropyl decyl hydroxypropyl ester gasification, stearic acid diethylaminoacetamide, stearic acid dinonylaminopropionamide, lanolin derivative Quaternary ammonium salts, etc. The amphoteric surfactants may be exemplified by a carboxyl beet domain type, a guanyl beet domain type, a % based beet domain type, a hydroxy contiguous beet domain type, a stilbene contiguous beet domain type, and a phosphoric acid. Examples of the sugar beet type, the aminocarboxylate type, the imidazoline derivative type, the guanamine type, etc. Examples of the nonionic surfactant include propylene glycol fatty acid ester, glycerin fatty acid ester, and polyglycerin fatty acid ester. Sorbitol fatty acid ester, Ρ0Ε sorbitan fatty acid ester, p〇E sorbitol fatty acid ester, Ε0Ε glycerin fatty acid ester, P0E alkyl ether, p〇E fatty acid vinegar, Ρ0Ε hardened kenaf oil, Ρ0Ε.Ρ0Ρ Copolymer, p〇Ep〇p=yl ether, polyether modified decane decanoic acid alkyl decylamine, alkyl amine oxide dihydrogenated soybean lecithin, etc. Natural surfactants may be exemplified by lecithin and saponin. , saccharide surfactants, etc. (Examples of hydrazine polyols, sugars include: ethylene glycol, monoethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, gan 31 200902033 oil, diglycerin, poly Glycerol, 3-mercapto-1,3-butanediol 1,3-butanediol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylose, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed heterosexuality Sugar, sulfated trehalose, amylopectin, etc. It is also possible to use chemical modifications thereof, etc. (8) Examples of the polymer are: acrylate/mercapto acrylate copolymer. Today only 兮<久', Vinyl acetate/crotonic acid copolymer (bashi > 28-1310, manufactured by NSC Co., Ltd.), vinyl acetate vinegar/crotonic acid/vinyl neodecanoate copolymer (28-2930, NSC Co., Ltd.), methyl vinyl shoddenic acid half ester (force, y b k 7 7 ES, manufactured by ISP), t-butyl acrylate / ethyl acrylate / methacrylic acid copolymer -, manufactured by BASF Co., Ltd.), vinylpyrrolidone / vinyl acetate / vinyl propionate copolymer (children only: ^ one melon VAP, manufactured by BASF Co., Ltd.), vinyl acetate / crotonic acid copolymer (small Seven 7 Bu CA, manufactured by BASF Co., Ltd.), acetic acid Vinyl ester/crotonic acid/vinylpyrrolidone copolymer (small 匕7v CAP, manufactured by BASF Corporation), vinylpyrrolidine _/acrylate copolymer (small 匕, 7k 7 夕, manufactured by BASF Corporation) ), acrylate/acrylamide copolymer (a small cloth, as shown in 8), vinyl acetate/butyl maleate/isobornyl acrylate copolymer, eight , / Yu Yi, ISP Co., Ltd.), fluorenyl vinyl polymer (manufactured by BF Goodri ch), acrylic acid alkyl methacrylate (弋S ak >, Goodrich Anionic polymer compound such as Manufactured; or an acetic acid amphoteric compound of methacrylic acid dialkylaminoethane® polymer (a 32 200902033, 7 7-7, manufactured by Mitsubishi Chemical Corporation), octyl propylene acrylate An amphoteric polymer compound such as decylamine/acrylic acid hydroxypropyl S/butyl cyanoacrylate copolymer (manufactured by AMPHOMER, NSC Co., Ltd.); vinyl group. Quaternary carbide of pirone ketone/dimethylaminoethyl methacrylate (GAFQUAT, manufactured by ISP), methyl vinyl vaporized imidazole rust/vinyl pyridoxone copolymer (baby 匕, BUSF) A cationic polymer compound such as manufactured by Co., Ltd.; polyvinylpyrrolidone (small smear only 17-small K 'BASF Co., Ltd.), vinyl hydrazine ketone ketone /

Ethyl acetate copolymer (Kaiyiyi: 7-^VA, manufactured by BASF Co., Ltd.), vinylpyrrolidone/dimethylaminoethyl methacrylate copolymer (: 7 water) 7-937, manufactured by ISP ), non-ionic polymer compounds such as vinyl caprolactam/vinylpyrrolidone/dimethylaminoethyl methacrylate copolymer (: 7 water 17-VC713, manufactured by ISP), etc. The polymer compound is also suitable for use. Cellulose and its derivatives, keratin and collagen or their derivatives, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, horn Cucurbita, high methoxyl pectin, low methoxyl pectin, guar gum, ala conversion, crystalline cellulose, arabinogalactan, karaya gum, yellow nautical, sea test, albumin, cheese Protein, curdlan, gellan gum, dextran, etc. (9) Examples of physiologically active ingredients? As a physiologically active ingredient, in the case of application to the skin, those which can bring some kind of physiology to the skin can be cited. Active substance. For example, List whitening ingredients, immunostimulating agents, anti-aging agents, anti-UV agents, body lotions, stretching agents, anti-oxidation know & Mao Xiaoying, moon hair, moisturizer, blood circulation promoting agent, 33 200902033 antibacterial agent, A bactericide, a desiccant, a cold sensitizer, a warming agent, a vitamin, a gas-based acid, a wound healing promoter, a stimulating emollient, an analgesic, a cell activating agent, an enzyme component, etc. as a suitable distribution for these physiological activities Examples of ingredients include, for example, backgrass extract, avocado extract, earthen mountain extract, hollyhock extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo extract , bactericidal extract, turmeric extract, oolong tea extract, turmeric extract, echinacea extract, scutellaria extract, scutellaria extract, berberine extract, barley extract, small forsythia extract, wild sesame Extract, cardamom extract, orange extract, seawater dry matter, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, coriander extract , Artemisia annua L. extract, licorice extract, hibiscus extract, Pyracantha extract, cinchona extract, cucumber extract, guanosine, hazelnut extract, mountain white bamboo extract, Sophora flavescens extract, walnut extract , grapefruit extract, hydrangea extract, spirulina extract, mulberry extract, gentian extract, black tea extract, yeast extract, burdock extract, black rice fermentation extract, rice germ oil, polymer grass Extract, Collagen, Bilberry Extract, Asarum Extract, Bupleurum Extract, Umbilical Cord Extract, Sage Extract, Soap Extract, Bamboo Extract, Hawthorn Extract, Pepper Extract, Xiangyi Extract, Rehmannia Extract, Purple Root Extract, Perilla Extract, Eucalyptus Extract, Spiraea Extract, Peony Extract, Caladium Root Extract, White Birch Extract, Euphorbia Extract, Ivy Extract, Western hawthorn extract, elderberry extract, yarrow extract, western mint extract, sage extract, mallow extract, Chuanxiong extract, sauerkraut extract, soy extract , jujube extract, thyme 34 200902033 extract, tea extract, clove extract, thatch extract, dried tangerine peel extract, angelica extract, calendula extract, peach extract, eucalyptus extract, surimi Grass extract, tomato extract, natto extract, ginseng extract, garlic extract, wild rose extract, hibiscus extract, wheat meringue extract, parsley extract, honey, witch hazel extract, daisy extract , fragrant tea extract, bisabolol, alfalfa extract, dandelion extract, butterbur extract, alfalfa extract, fake leaf extract, grape extract, propolis, loofah extract, safflower extract , Peppermint Extract, Linden Extract, Peony Extract, Hop Extract, Pine Extract, Horse Chestnut Extract, Water Barley Extract, Sapindus Extract, Fragrant Bee Flower Extract, Peach Extract, Cornflower Extract, Eucalyptus Extract, Saxifrage Extract, Coix Seed Extract, Artemisia Extract, Lavender Extract, Apple Extract, Lettuce Extract, Screw Extract, milk vetch extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract and the like. In addition, there may be mentioned: biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed shell film; Amino acid, peptide, milk _, urea, 吼 secret 峨, sugar beet, whey, trimethylglycine and other thirst-quenching ingredients; neurolipids, spirulina, sphingosine, cholesterol, cholesterol

=./Equivalent oily ingredients; coffee acid, Gan LP Ganzhuo: anodic acid, gasification solution _, guaiac blue oil hydrocarbon, mouth: P y / and other immune activators; vitamin J vitamin c, vitamin D, vitamin E, $, vitamins, niacinamide, vitamin c vinegar, etc.,: bow, biotin, bismuth and other vitamins, allantoin, di-acetic acid 200902033 diisopropylamine, 4-isomethylcyclohexanoate Fermentation expectant carotene, flavonoids, tannins, lignin, soap and other antioxidants; α_ acid, β-iron and other cell activators; γ_ oryzanol, vitamin ε derivatives and other blood circulation Retinol, retinol charm and other wound healing agents; albumin, kojic acid, placenta extract, sulphur, county flower acid, dioleic acid, condensed money, Weigan to whitening agent; bellows, licorice extract $, capsaicin, sterol, simmered garlic extract, cough HCl, DLt, monthly test, DL-oc-tocopherol acetate, niacin, nicotinic acid derivative, calcium pantothenate, D-panthenol, acetamidine Base ubiquinone ethyl ether, biotin, allantoin, isopropyl decyl benzene, estradiol, ethyl estradiol, Kapp gas recording, phenyl violet money, phenyl sea HCl Ming, Xi Li eleven, off brain, salicylic acid, citric acid fragrant _ amine, simmered acid vanillyl amide, mouth y y y, today, 'pentadecanoic acid glyceride, L-menthol, single Nitroguaiacol, resorcinol, 7-aminobutyric acid, benzethonium hydrochloride, mexiletine hydrochloride, auxin, estrogen, canthaxanthin, cyclosporin, zinc pyrithione, t Oral hair dyes such as 17 small thousand ^ ^, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil, and indigo 4 extracts. (10) Examples of antioxidants include sodium hydrogen sulfite, sodium sulfite, erythorbic acid, diarrhea, thiodipropionate, lauric acid, tocopherol, f-phenyl guanidine, and guanidine Hydrogen guaiac acid, p-hydroxyanisole, butyl hydroxymethyl ether, dibutyl hydroxy toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, Plant extracts having antioxidant effects such as carotene, flavonoids, tannins, lignin, saponins, apple extracts, and clove extracts. 36 200902033 (11) Examples of the solvent include purified water, ethanol, lower alcohol, ether, LPG, carbon gas compound, N-fluorenyl ketone ketone, fluorine-substituted alcohol, and volatile linear smoldering , secondary Freon and so on. [Embodiment] In order to facilitate the review committee to have a more advanced understanding of the technical means and operation process of the present invention and to understand the embodiment with the drawings, the details are as follows. The present invention relates to a "brain cell proliferation promoting agent and a neurite outgrowth agent", "Preparation Example 1 of the neuroblast cell proliferation promoting agent of the present invention", and prepares a column of the whole parasitic plant Cistanche tubulosa (& For example, if the extract is in the form of /%// (hereinafter referred to as "Cistanche tubulosa"), the preparation includes the following steps: Step 1: Extraction; take a tube of fresh stem of Cistanche tubulosa l〇kg Dip into the water of 8 times the amount of the sheet, and after immersing the sheet for 1 hour, it is decocted with water for 2 hours to form a mixture, and the mixture after decoction is filtered to obtain a first filtrate, and, in addition, After the filtration, the residue of the mixture is mixed with 4 times of 40% ethanol of the residue, and then boiled for 4 hours, and filtered again to obtain a second filtrate. The residue after filtering the second filtrate is subjected to the same steps as above. 'The residue was mixed with 4 times the amount of 40% ethanol of the residue, and then boiled for 4 hours and filtered. The operation was repeated twice to obtain a third filtrate and a fourth filtrate, and the first and second were combined. The third and fourth filtrates Concentrated, it is concentrated to a specific gravity of 1.05, yielding a final extract (by weight of the final extract was 6.2kg). Step 2: Purification; the above final extract is placed in water with a half weight of 37 200902033 (5 〇. 〇, so that it is dissolved in water, that is, the solution is obtained, and then the extract solution is placed - pre-filled = D-101 type macroporous adsorption resin in the adsorption column, the column is obtained with 2 times the amount of the fresh stem aqueous solution, and then - washing the adsorption column 'to obtain - the fresh stem 2 times the amount of ethanol solution L ' The first water-reducing and then cutting is performed to obtain a second aqueous solution of H alcohol, and the first and second aqueous solutions are combined, and after -concentration and drying procedures, the benzene is contained.

The extract of the compound (the weight of the extract is Q 865 (4). The content of the ascending buds of the echinacea is determined by high performance liquid chromatography (HPLC), and the conditions are as follows: the stationary phase is - (10) silk Wei bonding Dream gel, mobile phase is m 15% aqueous acetic acid solution (30:70), its flow rate is 1 ml / min, its inspection is long for the coffee ship. Each scale is taken in 60 X vacuum to dry 24 (four) - pine cone chrysanthemum and a class Leaf asparagine, dissolved in a 50% sterol, formulated into a control solution (the concentration of the control solution is 1 mL of solution containing 〇·lmg solute). While ultrasonically treated, it will contain the phenylethyl alcohol. 50 mg of the extract of the glycoside compound was dissolved in a 邠mL volumetric flask containing an appropriate amount of 5% methanol, and 50% methanol was further added to the above solution, and adjusted to a 25-inch scale, and the solution was accurately weighed to about 1 niL. Put in a 10mL volumetric flask, add 5 (U methanol to the mark, and finally filter with a membrane), then prepare a sample solution. w separately measure 5μ1 of the control solution and the sample solution, respectively Inject into a high performance liquid chromatograph and measure separately The 5%, the class of the content of the echinacoside in the extract is 37.5%, the class of the content of the echinacoside and the genus 38. The content of scutellarin in the extract is 9.0%. According to the second embodiment and the third embodiment, each is an echinacoside (in the present embodiment, the echinacoside is limited to the nectar pharmaceutical industry. The company's manufacture) and a class of pure lutein (in this example, the class of leaf asparagine purely manufactured by Xinghui Pharmaceutical Industry Co., Ltd.) on the proliferation of neuroblasts (see the second figure) Experiment 1: Evaluation method for evaluating the proliferation of nerve bud cells, a human neurotooth cell (the nerve bud cells are one) was suspended in an MEM medium (the MEM medium contained 1% FCS, one In the case of lOOimits/mL penicillin and a streptomycin, 5 ml of the above suspension is added to each of the 6 删11k, and then added to the various examples of the composition - the tube meat Secret extract, cultured for 5 days' then determined by MTT To evaluate the cell proliferation degree on the 5th day of culture, the result is as shown in the first step, and the extract of Example 2 and the actual Guan 3 was tested according to the sample step, as shown in the second figure. - As shown in the figure, when the Μττ assay is used to determine the proliferative degree of the cells, the 官 发 四 四 四 四 四 四 四 四 四 四 四 四 四 四 四 四 四 四 四 当 当 当 当 当 当 当 当 当 当 当 当 当 当When 〇/zg/mL, the proliferation rate of the nerve bud cells is 7%. When the concentration of the extract of Cistanche tubulosa is 3G#g/mL, the cell proliferation degree of the god (four) is pass, and thus the silk can be t (four): Even if the transfusion of bud cells does not occur in the lesion state, the extract is still responsible for the proliferation of human neuroblasts 39 200902033 cells (SK-N-SH), in addition, as measured by MTT as shown in the second figure When the proliferation degree of the nerve bud cells was measured, it was found that the echinacea popularizes the concentration-dependent neuroblast cell thinning effect, especially the pineal chrysanthemum bitterness at 1//g/mL and 1〇(4). This concentration shows a difference of -, which can also be confirmed. Even if the neuroblast has no lesions The state of the styrene-based material was ^ 'In particular, the Echinacea limb pain cohosh also has the effect (iv) proliferation of people over bud cells (SK-N-SH). Experiment 2: Evaluation of neurite outgrowth The human neuroblasts (SK_N_SH) were suspended in -MEM medium (containing -10% FCS, -1〇〇units/mL penicillin, and (10) #gAnL streptomycin). Add a 鼬丄 述 混 ' ' ' シ シ シ シ シ シ シ ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' The results were recorded in the same phase, and the results were as shown in the third figure. Further, the extracts of Example 2 and Example 3 were also tested in the same manner, and the results are shown in Fig. 4 and Fig. 5, respectively. As shown in the third figure, although the control group showed a cell proliferation phenomenon, the neurite outgrowth was not observed at all, and in contrast, the tuberculosis extract group was observed. More neurite outgrowth (refer to the case of the solid coil in the third figure), especially when the concentration of the Cistanche tubulosa extract is 30//g/mL, you can find the sacred food prominence and adjacent The cells form a network, and it can be confirmed that the tube extract is also in a concentration-dependent neurite outgrowth even in the state where the neuroblasts do not have lesions. 40 200902033 In a fine picture, although the control group showed its appearance, the _2='^果菊(4th) and the extension #U figure which can not be observed by the protrusions on the silk screen can be Bribe to more neurite out of the fourth and fifth pictures of the virtual coil), as in the -two A, fruit group, at low concentrations, the two observations and adjacent The cells form a network (in the case of reference circle/two), * this confirms that even in the state where the nerve bud cells are not issued, the fresh B-type compound, especially the steel and the leaf The Lang sample has a concentration to promote the Ginseng: the role of stretching. ~ Dogs The following is an example of a formulation of a neuroblast growth promoter in the present invention, but the formulation is not intended to limit the present invention. _ 53.0% by weight 20.0 10.0 16.0 0.5 0.5 Formulation Example 1: Chewing gum, sugar, gum base, glucose, water, spices, nerve bud cell proliferation promoter, etc.

100.0 Weight ° / 〇 Formulation Example 2: Chewable tablets around raw water granulated sugar 40. 0% by weight 20.0 41 200902033 Glucose 20.0 Gelatin 4.7 Water 9.68 Grape juice 4.0 Grape flavor 0.6 Pigment 0.02 Neuroblast cell proliferation promoter 1.0, etc. 100.0% by weight Formulation example 3: candy granules 50. 0% by weight leeches 33.0 water 14.4 organic acids 2.0 spices 0.2 nerve bud cell proliferation promoter 0.4, etc. 100. 0% by weight Formulation Example 4: yoghurt (hard. soft) Milk 41.5% by weight Skim milk 5.8 Sugar 8.0 agar 0.15 gelatin 0.1 lactic acid bacteria 0.005 42 200902033 nerve bud cell proliferation promoter, etc. 0.4 fragrance trace water tonic formula 5: refreshing beverage 100. 0% by weight fructose glucose sugar solution 30. 0% by weight emulsifier 0.5 nerve bud cell proliferation promotion Agent, etc. 0.05 Proper amount of purified water to make up the formula Example 6: Small dessert 100. 0% by weight Sugar 76.4% by weight Glucose 19.0 Sucrose fatty acid ester 0.050.2 Neuroblast proliferation promoter 0.5 etc. Purified water 3.9 Formulation Example 7: Soft capsule 100 0% by weight Grape seed 8重量% 54. 0重量% 54. 0重量% 54. 0重量% 54. 0重量% 54. 0重量% emulsifier 12.0 neuroblast cell thinning accelerator 1.0 43 200902033 and other formula 8: tablets lactose crystal cellulose starch decomposition products glycerol fatty acid ester neuroblast cell proliferation promoter, etc. 100. 0% by weight 54. 0% by weight 30.0 10.0 5.0 1.0 100. 0% by weight Formulation Example 9: granules for internal use (pharmaceuticals) Neuroblast cell proliferation promoter 1.0% by weight Lactose 30.0 Corn starch 60.0 Crystalline cellulose 8.0 Polyvinylpyrrolidone 1.0 100. 0 weight % Formulation Example 10: Makeup f Squalane 20. 0% by weight Bee fiber 5.0 Refined jojoba oil 5.0 Glycerin 5.0 Glycerol monostearate 2.0 Polyoxyethylene (20) sorbitan monostearate 2.0 44 200902033 Ester Neuroblast cell proliferation promoter, etc. 2.0 Preservatives Proper amount of flavored purified water to make up 100.0 Weight ° / 〇 Formulation example: lotion ethanol 5.0% by weight Glycerin 2.0 1,3-butanediol 2.0 Polyethylene ether ether 0.5 Sodium citrate 0.1 citric acid 0·1 neuroblast cell proliferation promoter 0.1 and other purified water to make up 100. 0% by weight Formulation Example 12 Body Lotion Macadamia Nut Oil 2.0% by Weight Myristate Octadecyl 10.0 Ester Methyl Phenyl Polyoxane 5.0 Dodecanol 3.0 Stearic Acid 3.0 Shark Liver Alcohol 1.0 45 200902033 Monostearate Monostearate 1.0 1.0 Oleic acid polyoxyethylene sorbose 2.0 Alcohol hydrogen added soybean fat filling 1.0 Neural decylamine 0.1 palmitol acid retinol ester 0.1 Preservative amount of Centella asiatica extract 1.0 Neuroblast cell proliferation promoter 1.0 and other 1,3-butyl Glycol 5.0 purified water to make up 100. 0% by weight Formulation Example 13: Emulsion squalane 4.0% by weight Vaseline 2.5 Recording sterol 2.0 Glycerin 2.0 Lipophilic glyceryl monostearate 1.0 Stearic acid 1.0 L-arginine 1.0 芽 增殖 增殖 增殖 神经 神经 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 Bud Cell Proliferation Promoter 0.5, etc. Lanolin 0.5 Avocado Oil 0.5 Pigment 1.5 Spice 22.0 100. 0% by weight Formulation Example 15: Cat Food Corn Flour 34 0% by weight Wheat flour 35.0 Meat meal 15.0 Beef fat 8.9 Salt 1.0 Carp extract 4.0 Neuroblast cell proliferation promoter 1.0 Etc. Taurine 0·1 Vitamins 0.5 Minerals 0.5 47 200902033 loo. 〇 Weight % 30.0% by weight 15.0 10.0 25.0 5. 0 5. 0 8.9 0.1 0.5 0.5 Formulation Example 16: Dog food cornmeal meat (chicken) Defatted soybean wheat flour facial paralysis neuroblast cell proliferation promoter and other animal oils and oils, sugar, vitamins and minerals such as 4 - 100.0% by weight

Mm (4) can provide tons of proliferation - even on the nerve buds = can also promote cell proliferation promoters and neurite outstretchers to advance into the gods, and the growth of dental cells, and can promote the expansion of nerve cells neurites, Promote the differentiation of nerve bud cells into nerve cells, even in the case of degeneration of nerve cells, the nerve bud cell thinning promoter and neurite outgrowth can effectively prevent the reduction of nerve cells 'by taking effective prevention of aging caused by the brain While improving brain function. The above detailed description is intended to be illustrative of a preferred embodiment of the present invention, and is not intended to limit the scope of the invention, which is not limited by the spirit of the invention disclosed herein. Equivalent changes and modifications of the modifications are intended to be included in the scope of the patents covered by the present invention. [Simple description of the diagram] The first figure is the proliferation effect of Cistanche tubulosa extract on human neuroblasts. The first panel is a graph showing the proliferative effect of echinacoside and flavonoids on human neuroblasts. The third picture is a photograph of the stretched state of the neurites caused by the extract of Cistanche tubulosa. ^The four-picture system is the extension of the neurite outgrowth caused by the pineal chrysanthemum. The fifth figure of the younger brother is the type of leaf swelling. The extension of the sinusoidal protrusion t > 7 ° ..., ,, [Description of the main components] 49

Claims (1)

200902033 X. Patent application scope: 1. A neuroblast cell proliferation promoter with monophenyleglucoside as an active ingredient. A neuroblast growth promoter according to the first aspect of the invention, wherein the δ-Hexylethanol compound comprises at least one of pineconate and one type of lutein. 3. A neuroblast proliferation promoter which is an active ingredient of a family of plant extracts. 4. A neuroblast cell proliferation promoting agent as described in claim 3, wherein the active ingredient is a stupid alcohol glycoside compound in the extract. 5. A neuroblast proliferation promoter as described in claim 4, wherein the stearyl glycoside compound is at least one of echinacoside and one type of lutein. 6. A neurite outgrowth agent having a phenylethanoid glycoside compound as an active ingredient. 7. A neurite outgrowth agent as described in claim 6 wherein the sulphuric ethanolaceous compound is at least one of a cupola chrysanthemum and a class of flavonoids. 8. A neurite outstretcher with an extract of the plant extract as an active ingredient. 9. A neurite outgrowth agent as described in claim 8 wherein the active ingredient is a phenylethanoid glycoside compound. 10. A neurite outgrowth agent as described in claim 9 wherein the stupid glucoside compound is at least one of echinacoside and one of the classes 50 200902033. 11· - The species may include the breastfeeding __ product of the human genus, the drug is a neuroblast cell proliferation promoter described in any of the scopes of the patent application range 5-1 to 5 or 10 in the scope of the patent application Any of the neurite outstretchers described as an active ingredient. The specific type of the skin may include a human skin extracting agent, which is effective in any one of the claims 1 to 5 of the patent application, or a patent application scope. The neurite outgrowth agent described in any one of the above is a 13· to mu product, and the food system is the first to be patented. The neuroblast cell proliferation promoter or the surrounding 6th to 1G described in the application. Any of the items in the item, the god, and the dog, act as an active ingredient. Animal-like feed 'The mammalian feed is the proliferation promoter described in the application, any of the items klUl to 5, or the _ _ _ _ _ _ _ _ _ _ _ _ _ Item 51