JP2008074792A - Composition for anti-fatigue use - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a nature-derived composition for anti-fatigue use having the function of ameliorating both of physical fatigue and mental fatigue. <P>SOLUTION: The composition for anti-fatigue use is characterized in containing as an active ingredient, Allium tuberosum's flower, seed and/or extract thereof. Further, this composition preferably contains as another active ingredients, at least one substance among ginseng, garlic, Eleutherococcus senticosus, turmeric, Momordica grosvenori, and Piper methysticum, Cistanche tubulosa and Cistanche salsa, and at least one extract thereof. In addition, this composition preferably also contains γ-aminobutyric acid. The composition may be included in pharmaceuticals, foods/drinks, skin care preparations, etc. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a novel anti-fatigue composition having an improving effect on both physical fatigue and mental fatigue.

  Eating, exercising and resting are considered important for maintaining human health (Health Japan 21 and Dietitian Activities, Japan Dietetic Association, 1st Publication, 2000). However, the current situation is far below the target value established in Health Japan 21, and improvement of lifestyle habits is desired.

  In the health Japan 21 mentioned above, moderate exercise is recommended for maintaining and promoting health, but physical fatigue and mental fatigue occur on the other hand, resulting in phenomena such as muscle fatigue and feeling of fatigue. There is a demerit that they are accumulated and are accumulated by continuous exercise.

  In other words, if you reduce the accumulation of fatigue due to exercise, you will be able to exercise more on a daily basis, and it will lead to maintenance of exercise ability and recovery in a short period in intense exercise, such as sports competitions etc. Conceivable.

  In addition, according to the results of a survey on the actual state of fatigue conducted by the National Institute of Public Health in 1999, 59.1% of the respondents answered that they are currently feeling fatigue. Physiology of fatigue and measurement / quantification method and relaxation by food Osamu Enomoto FOOD Style 21 Vol. 6 No. 3 2002), it can be easily imagined that fatigue is a daily problem of many people as well as during exercise.

  In addition, not only physical fatigue, but also chronic fatigue syndrome, in which chronic sleep deficiency due to strong psychological stress is noticeable, is regarded as one of the social problems. In this chronic fatigue syndrome, in many cases, weakness in work and malaise causes a decrease in motivation for work and motivation for daily activities. Furthermore, the chronic fatigue syndrome is becoming younger, including the treatment of underlying diseases, such as drug-induced depression due to the use of antihypertensive drugs. From this point of view, the importance of so-called “quality of life” is advocated in the medical field.

Against this background, various proposals have been made on foods and agents that can be expected to improve physical fatigue and mental fatigue.
For example, for those having an action to improve physical fatigue, the physical fatigue improving agent containing 2-ketoglutaric acid as an active ingredient (see, for example, Patent Document 1), the effect on physical fatigue using Cordyceps sinensis and kaikjin as active ingredients is enhanced. There is a fatigue-improving agent (for example, see Patent Document 3) containing as an active ingredient a prepared internal liquid (for example, see Patent Document 2), biotin, pantothenic acid, and carnitine. They are evaluating improvements in physical fatigue, but the effect on mental fatigue is not specified.

  On the other hand, as an invention having an effect of improving mental fatigue, there is a mental functional composition (for example, see Patent Document 4) containing a mixed component of caffeine, theanine and arginine contained in tea as an active ingredient. The effect on is not specified.

  Therefore, it is effective in improving both physical and mental fatigue, and has an action to improve not only fatigue due to exercise stress but also mental stress such as chronic fatigue syndrome. There is a need for a versatile fatigue improver that can be ingested.

Japanese Patent Laid-Open No. 10-175855 JP 2001-158747 A JP 7-233300 A JP 2002-322063 A

Under such a background, the present inventors have found that the components contained in leek seeds have an effect of improving both mental fatigue and physical fatigue, and have completed the present invention.
That is, an object of the present invention is to provide an anti-fatigue composition having an action of improving both mental fatigue and physical fatigue using a novel component as an active ingredient.

In order to solve the above-mentioned problems, an anti-fatigue composition of the present invention comprises an extract extracted from at least one of leek (Allium tuberosum) flowers and seeds, and at least one of leek flowers and seeds, At least one of them is an active ingredient.
Further, the anti-fatigue composition of the present invention comprises at least one of ginseng, garlic, sorghum, turmeric, rahan fruit, birch hippo, kankanikujuyo, and licorice, as well as ginseng, garlic, sorghum, turmeric, arahan fruit. In addition, it is preferable to further contain at least one of extracts extracted from at least one of birch, birch, birch and licorice as an active ingredient.
The anti-fatigue composition of the present invention is characterized by containing a pharmaceutical, a food or drink, and a skin external preparation.

Hereinafter, the present invention will be described in detail.
The anti-fatigue composition of the present invention is characterized by using leek (Allium tuberosum) and / or an extract thereof as an active ingredient.
The above “leek” is a perennial green-yellow vegetable belonging to the genus Liliaceae. The bulb is oval, with a short rhizome below the bulb. Divided into large numbers and stretches leaves. The leaf is 20-30 cm long and grows. The shape is linear and flat, not cylindrical like a leek. In summer, the flower stem is stretched about 30 to 40 cm from between the leaves. The flowering period is from August to October, and it has a hemispherical inflorescence with 20 to 40 small white flowers. It looks like there are 3 petals but 3 buds and 6 petals. There are 6 stamens and 3 cots. The ovary is said to be ripe and sprays a small black seed. Native to western China. Increase by strain or species. The whole plant has a unique odor, and the odor-causing substances are sulfur compounds such as allyl sulfide. It is known to contain abundant vitamins and minerals including vitamin A. The leaves are known to be strong and tonic with a crude drug called cucumber, and the seeds are known to be effective against lumbago, pollakiuria, etc. with a crude drug called cucumber.

The present invention is characterized by using leek flowers and seeds. At this time, both flowers and seeds may be used, or either one may be used. Of these, seeds are particularly preferred. This is because an active ingredient having an anti-fatigue function can be extracted at a higher concentration.
Moreover, in this invention, if it is a method using a leek flower or a seed, all the usage methods can be performed. At this time, leek flowers and seeds may be used as they are, but leek flowers and seed extracts are preferably used. This is because an active ingredient having an anti-fatigue action contained in leek flowers and seeds can be obtained at a higher concentration.
In addition, when an extract of leek seed (hereinafter referred to as “leek seed”) is used, a method for extracting an active ingredient from leek seeds is not particularly limited, and examples include polar solvent extraction and supercritical extraction.
Moreover, when extracting with a polar solvent, leek seeds may be used as an extraction raw material, but defatted leek seeds may be used. It is because an active ingredient can be extracted easily.
At this time, as a degreasing method, for example, the oil component may be separated by pressing the leek seeds, or after separating the oil component, the residual oil component of the pressed product is extracted and separated with a degreasing solvent (fat-soluble organic solvent). You may do it. Furthermore, leek seeds may be crushed and extracted and separated using a degreasing solvent (fat-soluble organic solvent).

  At this time, preferable degreasing solvents include n-hexane, acetone and the like. In particular, it is preferable to use n-hexane. This is because the extracted oil can be used as an edible oil and the defatted leek seed extract can be easily used as a food material. These degreasing solvents may be used alone or in combination of two or more.

  Furthermore, when extracting by polar solvent extraction, the polar solvent to be used is not particularly limited. For example, water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, acetic acid are used. Examples include ethyl, ether, hexane and the like. Of these, water and ethanol are preferred. In particular, it is preferable to use hydrous ethanol. These may be used alone or in combination of two or more.

  When water is used as the extraction solvent, the extraction temperature is 20 to 100 ° C., preferably about 80 to 100 ° C. This is because if the extraction temperature is too low, it is difficult to extract active ingredients. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water and the like can be used.

  When hydrous ethanol is used as the extraction solvent, the ethanol concentration is preferably 30 to 90 wt%. This is because if the amount is less than about 30 wt% or exceeds 90 wt%, the extraction amount of the active ingredient tends to decrease. The extraction temperature is 20 to 80 ° C, preferably about 50 to 80 ° C. In addition, the water-containing ethanol extraction may be repeated at various concentrations in order to improve the content of the active ingredient.

  In addition, when extracting with a polar solvent, any extraction method such as continuous extraction, immersion extraction, countercurrent extraction, etc. can be employed as the extraction method, and any apparatus can be used at room temperature or under reflux heating. Can do. Moreover, you may combine the said extraction method etc.

As a specific method, an extraction raw material is put into a treatment tank filled with an extraction solvent, and an active ingredient is eluted while stirring. For example, when water or water-containing ethanol is used as the extraction solvent, the extraction is performed for about 30 minutes to 5 hours using a polar solvent of about 5 to 100 times (weight ratio) of the extraction raw material. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying and purification according to a conventional method to obtain an anti-fatigue composition.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like, but they are not used in large quantities when added to foods. It may be used unpurified.

  Moreover, when extracting by supercritical extraction, the supercritical fluid to be used is not specifically limited, For example, a carbon dioxide, water, etc. are mentioned. In addition, these may use only 1 type and may use 2 or more types together. Of these, carbon dioxide is particularly preferred. It is because an active ingredient can be extracted more easily. The extraction method at this time may be performed by a known method.

  In addition, the anti-fatigue composition of the present invention further contains at least one of ginseng, garlic, sorghum, turmeric, rahan fruit, birch, birch, licorice, and extracts thereof as active ingredients. Can do. This is because the anti-fatigue action can be further enhanced by further containing these.

  Ginseng is native to the Korean peninsula and grows naturally in the northeastern part of China and the Primorsky Krai. Currently, more than 70% of the total is cultivated in Korea and China, but it has also been cultivated in Japan since the Edo period. In Japan, it has been called “Kinseng” for a long time, but in Korea it is simply called “Ginseng”. The essential medicinal part is the root and the useful ingredient is a saponin group called ginsenoside, which is effective for nourishing tonic and has been loved for a long time.

  Garlic (蒜: 蒜, Allium sativum) is a perennial plant belonging to the family Liliaceae, which has a nourishing tonic effect and is used in energy drinks and health foods.

Ezoukogi (Japanese name: Ezoukogi, Sakai Goka / English name: Siberian ginseng, Eleutherococcus / Chinese name: Sashigoka) is a deciduous shrub of the same scallop family, such as ginseng, udd and cod buds, Native to harsh natural environments such as northeastern Hokkaido,
It is a plant that cannot be grown by artificial cultivation.
Ezoukogi has been widely known in China for about 2,000 years ago, and has been gaining attention in Europe and America since the former Soviet scientists announced the utility of the extract in the 1960s. It is known that Ezoukogi has physical strength, athletic ability improving action, nourishing tonic action and anti-stress action.

  Turmeric is a perennial plant belonging to the family Ginger, and generally prefers high temperature and humidity, and grows naturally from the tropics to subtropics of Asia, Africa, and Central and South America, mainly in South Asia. Of these, spring turmeric, autumn turmeric, and gadju (purple turmeric) are often used. Turmeric is regarded as a liver medicine and is said to cause fatigue by restoring weak liver functions.

Rakanka is a cucurbitaceae plant, a precious fruit that grows only around Guilin, China, and is said to have nourishing tonic.
Hippopotamus (Piper methysticum) has long been used as a drink in Malaysia and Polynesia and is now widely used as a relaxing herb and is known for its anti-stress activity and transient excitability.

Cistanche Tubulosa is a plant belonging to the genus Cantaceae that parasitizes the red and willow tree in the Taklamakan Desert in the Uygur Autonomous Region, and is a regular food for people in the South Road of the Silk Road and the Hotan region to survive in harsh desert environments. It is a food that has been supplemented with physical strength and is said to have nourishing tonic.
Gypsophila is a dried meaty stem of the crested crabs (Cistanche salsa (CAMey) G. Beck) and has a tonic effect.

When the composition for anti-fatigue of the present invention further uses ginseng, sorghum, turmeric, rahan fruit, ophthalmic tree, birch, kankanikuju, licorice, etc., the method of using these raw materials is not particularly limited, all Can be used. At this time, these dry powders may be used as they are, but it is preferable to use these extracts. This is because these active ingredients can be obtained at a higher concentration.
Moreover, the method of extracting an active ingredient from these is not specifically limited, Polar solvent extraction, supercritical extraction, etc. are mentioned.

  Furthermore, when extracting by polar solvent extraction, the polar solvent to be used is not particularly limited. For example, water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, acetic acid are used. Examples include ethyl, ether, hexane and the like. Of these, water and ethanol are preferred. In particular, it is preferable to use hydrous ethanol. These may be used alone or in combination of two or more.

  When water is used as the extraction solvent, the extraction temperature is 20 to 100 ° C., preferably about 80 to 100 ° C. This is because if the extraction temperature is too low, it is difficult to extract active ingredients. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water and the like can be used.

  When hydrous ethanol is used as the extraction solvent, the ethanol concentration is preferably 30 to 90 wt%. This is because if the amount is less than about 30 wt% or exceeds 90 wt%, the extraction amount of the active ingredient tends to decrease. The extraction temperature is 20 to 80 ° C, preferably about 50 to 80 ° C. In addition, the water-containing ethanol extraction may be repeated at various concentrations in order to improve the content of the active ingredient.

  In addition, when extracting with a polar solvent, any extraction method such as continuous extraction, immersion extraction, countercurrent extraction, etc. can be employed as the extraction method, and any apparatus can be used at room temperature or under reflux heating. Can do.

As a specific method, an extraction raw material is put into a treatment tank filled with an extraction solvent, and an active ingredient is eluted while stirring. For example, when water or water-containing ethanol is used as the extraction solvent, the extraction is performed for about 30 minutes to 5 hours using a polar solvent of about 5 to 100 times (weight ratio) of the extraction raw material. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying and purification according to a conventional method to obtain an anti-fatigue composition.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like, but they are not used in large quantities when added to foods. It may be used unpurified.

  Moreover, when extracting by supercritical extraction, the supercritical fluid to be used is not specifically limited, For example, a carbon dioxide, water, etc. are mentioned. In addition, these may use only 1 type and may use 2 or more types together. Of these, carbon dioxide is particularly preferred. It is because an active ingredient can be extracted more easily. The extraction method at this time may be performed by a known method.

The anti-fatigue composition of the present invention can further contain γ-aminobutyric acid and / or a derivative thereof as an active ingredient. It is because mental fatigue can be reduced especially by containing these.
Here, the derivatives of γ-aminobutyric acid in the present invention include salts, esters and the like, and more specifically, nicotinoyl γ-aminobutyric acid, γ-aminobutyric acid calcium salt, γ-aminobutyric acid sodium salt, Examples include γ-aminobutyric acid magnesium salt, γ-aminobutyric acid methyl ester, ethyl ester, propyl ester, isopropyl ester, butyl ester, tert-butyl ester, and the like. In addition, these may contain only 1 type and may contain 2 or more types.
Moreover, the manufacturing method of the said (gamma) -aminobutyric acid is not specifically limited, You may manufacture by a synthesis | combination and you may use a plant-derived thing.

In addition, when using plant-derived γ-aminobutyric acid / and derivatives thereof (hereinafter simply referred to as “γ-aminobutyric acids”), the plant as a raw material is not particularly limited. For example, rice, wheat, corn, Kimchi etc. are mentioned. These may be used alone or in combination of two or more. Of these, it is particularly preferable to use γ-aminobutyric acid derived from rice.
Moreover, when using rice as a raw material, the kind of rice is not specifically limited, For example, germ rice, brown rice, germinated brown rice, white rice, etc. can be used. In addition, these may use only 1 type and may mix and use 2 or more types.
Furthermore, the method for obtaining γ-aminobutyric acid from the plant is not particularly limited. For example, a method for purifying glutamic acid contained in the plant raw material by converting at least a part thereof into γ-aminobutyric acid by a converting enzyme. And a method obtained by fermenting a plant-containing material as a medium.

  The anti-fatigue composition of the present invention can be used as a material for various foods and drinks. Examples of food and drink include edible oil (salad oil), confectionery (gum, candy, caramel, chocolate, cookie, snack, jelly, gummy, tablet confection etc.), noodles (soba, udon, ramen etc.), dairy products ( Milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.) and health foods (tablets , Capsules, etc.) and nutritional supplements (nutrient drinks, etc.). The anti-fatigue composition of the present invention may be appropriately blended with these foods and drinks.

  These foods and drinks can be blended with various components depending on the type, for example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid. Acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, Food materials such as carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances and preservatives can be used. In addition, the anti-fatigue composition having a health maintenance function includes other antioxidants and health food ingredients such as antioxidants (reduced ascorbic acid (vitamin C), vitamin E, reduced form). Glutatin, tocotrienol, vitamin A derivative, lycopene, lutein, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sesamin, lignans, catechin, isoflavone, chalcone, tannins, flavonoids, Coumarin, isocoumarins, blueberry extract), health food ingredients (V. (vitamin) A, V.B1, V.B2, V.B6, V.B12, VC, VD, VE, VP, choline, niacin, pantothenic acid , Calcium folate, EPA, oligosaccharide, dietary fiber, squalene, soy lecithin, taurine, donariella Protein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium, potassium, heme iron, oyster meat extract, chitosan, chitin oligosaccharide, collagen, chondroitin, turmeric, licorice, kokushi, kehi, hawthorn , Ginger, ganoderma, swordfish extract, suppon, licorice, kokushi, keihi, hawthorn, ginger, ganoderma, plantain, chamomile, chamomile, dandelion, hibiscus, honey, boren, royal jelly, lime, lavender, rosehip, rosemary, Sage, bifidobacteria, faecalis, lacris, wheat germ oil, sesame oil, perilla oil, soybean oil, medium chain fatty acid, agaricus, ginkgo biloba extract, turmeric, chondroitin, brown rice germ extract, litchi, onion, DHA, EPA DPA, green tea, cordyceps, garlic, bee, papaya, puer, propolis, meguri tree, yabushitake, royal jelly, saw palmetto, hyaluronic acid, collagen, gabba, harpseal oil, shark cartilage, glucosamine, lecithin, phosphatidylserine, Tanachi Carrot, Mulberry Leaf, Soybean Extract, Echinacea, Ezoukogi, Barley Extract, Olive Leaf, Olive Seed, Gymnema, Banaba, Salacia, Garcinia, Chitosan, St. John's Wort, Jujube, Carrot, Passion Flower, Broccoli, Placenta, Barley , Grape seed, peanut seed coat, bilberry, black cohosh, maria thistle, laurel, sage, rosemary, raffma, black vinegar, bitter gourd, maca, safflower, flax, oolong tea, flower spine, caffeine, capsaicin, Shirooligosaccharide, Glucosamine, Buckwheat, Citrus, Dietary fiber, Protein, Prunes, Spirulina, Barley young leaves, Nucleic acid, Yeast, Shiitake, Plum meat, Amino acid, Deep sea potato extract, Noni, Oyster meat, Suppon, Champignon, Psyllium, Acerola, Pineapple, Banana, Peach, Apricot, Melon, Strawberry, Raspberry, Orange, Fucoidan, Meshimakobu, Cranberry, Chondroitin sulfate, Zinc, Iron, Ceramide, Silk peptide, Glycine, Niacin, Chesttree, L-cysteine, Red wine leaf, Millet, Horsetail, biotin, centella asiatica, lotus cup, pynogenol, buffalo, rhubarb, clove, rosemary, catechin, puer, citric acid, brewer's yeast, merirot, black zinger, ginger, gadget, nutuki Sesame, becouge, tocotrienols, lactoferrin, cinnamon, buckwheat buckwheat, cocoa, yuzu seed extract, perilla seed extract, litchi seed extract, evening primrose extract, black rice extract, α-lipoic acid, gabba, fresh coffee bean extract, kiwi extract, kiwi Seed extract, Unshu orange extract, red ginger extract, astaxanthin, walnut extract) and the like.

  As a specific production method, the anti-fatigue composition is spray-dried or freeze-dried together with powdered cellulose, and this is easily incorporated into foods and drinks (instant foods, etc.) by making it into powders, granules, tablets or solutions. Can do. Further, the anti-fatigue composition can be dissolved in, for example, fats and oils, ethanol, glycerin, or a mixture thereof to form a liquid and added to a beverage or added to a solid food. If necessary, it can be mixed with a binder such as gum arabic or dextrin to form a powder or granules, which can be added to beverages or solid foods.

  As the addition amount when the anti-fatigue composition of the present invention is applied to foods and drinks, the main purpose is to maintain health, so the total content of active ingredients is 1 to 20 wt% with respect to the food and drinks. Preferably there is.

  The anti-fatigue composition of the present invention may be used as a raw material for drugs (including pharmaceuticals and quasi drugs). It can be manufactured by appropriately blending the anti-fatigue composition of the present invention with a raw material for a pharmaceutical preparation. Examples of the preparation raw material that can be blended in the anti-fatigue composition of the present invention include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cocoa butter, hydrogenated vegetable oil, kaolin, Talc, etc.), binder (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrant (sodium alginate, agar, carbonate) Sodium hydride, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, starch, lactose, gum arabic powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (No. Quaternary ammonium base, sodium lauryl sulfate, etc. , Adsorbent (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, and the like) and the like.

The administration method of the anti-fatigue composition according to the present invention can generally be administered orally in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules, liquids and the like. It may be administered parenterally. When administered as a parenteral agent, it can be applied in the form of a solution, or in the form of a haptic agent, lotion agent, ointment, tincture, cream, etc. with the addition of a dispersant, suspension, stabilizer, etc. can do.
The dosage may vary depending on the administration method, medical condition, age of patient, etc., but for adults, it can be generally administered as 0.5 to 1000 mg as an active ingredient per day, and for children, about 0.5 to 500 mg can be usually administered.
The compounding ratio of the anti-fatigue composition can be appropriately changed depending on the dosage form, but is usually about 0.3 to 15.0 wt% when administered orally or by mucosal absorption, when administered parenterally. Is preferably about 0.01 to 10 wt%. In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range.

The anti-fatigue composition of the present invention of the present invention can be expected to have an anti-fatigue action even when used as an external preparation for skin (including cosmetics, pharmaceuticals and quasi drugs).
Examples of the external preparation for skin to which the anti-fatigue composition of the present invention can be formulated include, for example, emulsion, soap, face wash, bath preparation, cream, emulsion, lotion, eau de cologne, shave cream, shave lotion. , Cosmetic oil, suntan / sunscreen lotion, powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow, blusher, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, shampoo, Rinse, hair dye, dispersion liquid, cleaning agent, etc. are mentioned.
Examples of the form of a pharmaceutical or quasi-drug that can be blended with the anti-fatigue composition of the present invention include ointments, creams, and liquids for external use.

In addition to the anti-fatigue composition according to the present invention, the external preparation for skin of the above-described form includes ingredients, oils, and high-grade ingredients to be blended in external preparations for skin such as cosmetics and quasi-drugs as long as the anti-fatigue action is not impaired Alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, and the like can be blended.
Examples are listed below, but the present invention is not limited to these examples.

(1) Examples of oil components Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearic acid Isocetyl, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoaralkyl neopentanoate, glyceryl tri (capryl / caprate), trimethylolpropane tri-2-ethylhexanoate, trimethylol triisostearate Propane, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isolaurate Stearyl, isotridecyl myristate, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl linoleate, octyldodecyl isoleate, isopropyl isostearate Cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethyleneglycol dioctanoate , Ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol di (capryl / capric acid), propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, triisopalmitine Glyceryl acid, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl decyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleic acid Ester, polyglycerol isostearate, dipropyl carbonate, charcoal Dialkyl (C12-18), triisocetyl citrate, triisoaralkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyl decyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, Trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, olein Cholesteryl acid, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, 12-stearoyl hydroxystearate Cetyl, 12-stearoyl-hydroxystearic acid stearyl 12-stearoyl-hydroxystearic acid isostearate, and the like.
Hydrocarbon oil phase components: squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum and the like.
Animal and vegetable oils and their hydrogenated oils, and naturally occurring waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated tallow, horse oil, hydrogenated horse oil, mink oil, orange luffy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils and Its hardened oil, avocado oil, almond oil, olive oil, cacao butter, kiwi seed oil, apricot oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil , Perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil , Vegetable oils such as sunflower oil, grape seed oil, jojoba oil, hardened jojoba oil, macadamia nut oil, medhome oil, cottonseed oil, hardened cottonseed oil, coconut oil, hardened coconut oil and its hardened , Beeswax, high acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax and montan wax.
Silicone oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethyl Siloxane / methylcetyloxysiloxane copolymer, dimethylsiloxane / methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic Examples include silicone, trimethylsiloxysilicic acid, and silicone RTV rubber.
Fluorine oil phase components: perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl / polyoxyalkylene co-modified organopolysiloxane, and the like.

(2) Examples of higher alcohols Lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol and the like can be mentioned.

(3) Examples of fatty acids Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid , Erucic acid, 2-ethylhexanoic acid and the like.

(4) Examples of UV absorbers Paraaminobenzoic acid, amyl paraaminobenzoate, ethyldihydroxypropyl paraaminobenzoate, glyceryl paraaminobenzoate, ethyl paraaminobenzoate, octyl paraaminobenzoate, octyldimethyl paraaminobenzoate, ethylene glycol salicylate, salicylic acid Octyl, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl paramethoxycinnamate, 2-ethylhexyl paramethoxycinnamate, mono 2-diparamethoxycinnamate Glyceryl ethylhexanoate, isopropyl paramethoxycinnamate, diethanolamine salt of paramethoxyhydrocinnamic acid, diisopropyl diisopropylcinnamic acid Stealth mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, Butylmethoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2-ethylhexyl-1-oxy) -1,3,5-triazine, 2- (2-hydroxy-5-methylphenyl) benzotriazole , Methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3, 3-diphenyl acrylate, phenylbenzimidazole sulfate, 3- (4-methylbenzylidene) can Examples include full, isopropyldibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2, 5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like, polymer derivatives, silane derivatives, and the like.

(5) Examples of powders and pigments Red 104, Red 201, Yellow 4, Blue 1, Black 401 and other dyes, Yellow 4 AL lake, Yellow 203 BA lake and other lake dyes, nylon powder , Silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder, yellow iron oxide, red iron oxide, black Colored pigments such as iron oxide, chromium oxide, carbon black, ultramarine and bitumen, white pigments such as zinc oxide, titanium oxide and cerium oxide, extender pigments such as talc, mica, sericite, kaolin and barium sulfate plate, titanium mica Pearl pigments such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, metal salts such as magnesium silicate, Inorganic powders such as Rica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, zinc undecylenate, bentonite, smectite, boron nitride, etc. It is done. There are no particular restrictions on the shape of these powders (spherical, rod-like, needle-like, plate-like, irregular shape, flake-like, spindle-like, etc.) and particle diameter. These powders are conventionally known surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil agent treatment, N-acylated lysine treatment, The surface treatment may or may not be performed in advance by polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment or the like.

(6) Examples of surfactants Anionic surfactants: fatty acid soap, α-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate , Alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloylalkyl taurine salt, N-acyl amino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, alkyl sulfoacetic acid Sodium, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate, etc. are mentioned.
Cationic surfactant: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride , Behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, and the like.
Amphoteric surfactants: carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type and the like.
Nonionic surfactant: propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE cured castor Oil, POE castor oil, POE / POP copolymer, POE / POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, and the like.
Natural surfactant: lecithin, saponin, sugar surfactant and the like.

(7) Examples of polyhydric alcohols and sugars Ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1, 3 -Butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan and the like. These chemically modified compounds can also be used.

(8) Examples of polymers Acrylic ester / methacrylic acid ester copolymer (plus size, manufactured by Kyoyo Chemical), vinyl acetate / crotonic acid copolymer (resin 28-1310, manufactured by NSC), vinyl acetate / croton Acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (rubymer) , BASF), vinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubicol VAP, BASF), vinyl acetate / crotonic acid copolymer (Rubiset CA, BASF), vinyl acetate / croton Acid / vinyl pyrrolidone copolymer (Rubiset CAP, manufactured by BASF), vinyl pyrrolidone / acrylate copolymer (Rubiflex, BA SF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxy vinyl polymer (Carbopol, BF Goodrich), anionic polymer compounds such as acrylic acid / alkyl methacrylate copolymers (Pemulene, BF Goodrich) and acetic acid amphoteric compounds of dialkylaminoethyl methacrylate polymers (Yukaformer, Mitsubishi Chemical) Amphoteric polymer compounds such as octyl acrylate acrylate / hydroxypropyl acrylate / butyl aminoethyl methacrylate copolymer (AMPHOMER, NSC), quaternized vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, ISP) , Methyl vinyl imidazolium chloride Cationic polymer compounds such as vinyl / vinylpyrrolidone copolymer (rubicoat, manufactured by BASF), polyvinylpyrrolidone (rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (rubiscol VA, manufactured by BASF) ), Nonionic polymer compounds such as vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP), vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP), etc. There is. Cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharide, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabino Naturally derived polymer compounds such as galactan, gum karaya, gum tragacanth, alginic acid, albumin, casein, curdlan, gellan gum and dextran can also be suitably used.

(9) Examples of physiologically active ingredients Examples of physiologically active ingredients include substances that impart some physiological activity to the skin when applied to the skin. For example, whitening ingredients, anti-fatigue compositions, anti-aging agents, UV protection agents, slimming agents, tanning agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, and drying Agent, cooling sensation agent, warming sensation agent, vitamins, amino acids, wound healing promoter, stimulus relaxation agent, analgesic agent, cell activator, enzyme component and the like. Examples of these suitable ingredients include, for example, ashitaba extract, avocado extract, amateur extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, ages Extract, Echinacea leaf extract, Ogon extract, Oat extract, Oen extract, Barley extract, Hypericum extract, Oyster extract, Dutch mustard extract, Orange extract, Seawater dried product, Seaweed extract, Hydrolyzed elastin, Hydrolyzed wheat powder, Hydrolyzed silk , Chamomile extract, Carrot extract, Kawara mugwort extract, Licorice extract, Calcade extract, Oyster extract, Kina extract, Cucumber extract, Guanosine, Gardenia extract, Kumazasa extract, Clarae Kiss, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, tea extract, yeast extract, burdock extract, fermented rice bran extract, rice germ oil, comfrey extract, collagen, bilberry extract, saicin extract, psycho extract Extract Kizuta extract, hawthorn extract, elderberry extract, yarrow extract, mint extract, sage extract, mallow extract, senki Sue extract, assembly extract, soybean extract, tiso extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, touki extract, toshinsen extract, tonin extract, spruce extract, dokudami extract, tomato extract, natto extract, carrot extract, garlic extract, Novara extract, hibiscus extract, bacmond extract, parsley extract, honey, hamamelis extract, parietalia extract, toad extract, bisabolol, loquat extract, dandelion extract, burdock extract, bukuro extract, butcher bloom extract, grape extract, propolis, loofah extract, Safflower extract, peppermint extract, bodaige extract, button extract, hop extract, pine extract, marronnier extract, mizubasho Kiss, Mukuroji extract, Melissa extract, Peach extract, Cornflower extract, Eucalyptus extract, Yukinoshita extract, Yokuinin extract, Artemisia extract, Lavender extract, Apple extract, Lettuce extract, Lemon extract, Lotus root extract, Rose extract, Rosemary extract, Roman chamomile extract And royal jelly extract.
Biopolymers such as deoxyribonucleic acid, mucopolysaccharide, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, hydrolyzed eggshell membranes, amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidonecarboxylate , Moisturizing ingredients such as betaine, whey, trimethylglycine, oily ingredients such as sphingolipid, ceramide, phytosphingosine, cholesterol, cholesterol derivatives, phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, Anti-fatigue composition such as hydrocortisone, vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C Vitamins such as thiol, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoid, flavonoid, tannin, lignan, saponin, α-hydroxy acid, β-hydroxy Cell activators such as acids, blood circulation promoters such as γ-oryzanol and vitamin E derivatives, wound healing agents such as retinol and retinol derivatives, arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, glutathione Whitening agent, cephalanthin, licorice extract, red pepper tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivative, calcium pantothenate, D- Pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol, ethinylestradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, takanal, camphor, salicylic acid, nonyl acid vanillylamide, nonanoic acid vanillylamide, pyroctone Olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcin, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormone, cantalis tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, monostearic acid Examples include hair growth agents such as polyoxyethylene sorbitan, mint oil, and Sasanishiki extract.

(10) Examples of antioxidants: sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolyl biguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxy Examples include plant extracts having an antioxidant effect such as toluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoid, flavonoid, tannin, lignan, saponin, apple extract and clove extract.

(11) Examples of solvents Purified water, ethanol, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile linear silicone, next-generation chlorofluorocarbon and the like.

Example Production of leek seed extract Leek seeds were pulverized and defatted with hexane in an amount 3 times (weight ratio) of leek seeds at an extraction temperature in the range of 40 to 50 ° C. with stirring for 1 hour. This operation was performed twice. Thereafter, the hexane extraction residue is naturally dried overnight, the weight of the dried product is measured, and then the extraction temperature is 80 to 100 ° C. with 50% by weight aqueous ethanol containing 5 times the amount (weight ratio) of the dried product. Extracted with stirring for 2 hours. Then, the residue was discarded by vacuum filtration, and the filtrate was concentrated with an evaporator and dried in vacuo at 60 ° C. overnight to obtain a leek seed extract (Example).

Test Example 1: Antipsychotic fatigue effect using reserpine model mouse (1) Experimental material The leek seed extract of the example (hereinafter also simply referred to as “leek seed extract” or “leek seed extract”) and male. Experiments were performed using 5-week-old ddy mice.

(2) Method Thirty mice were subjected to control group (Cont.), Reserpine group (Res), Res + leek seed extract 50 mg / kg group (Res + ATSE50), Res + leek seed extract 100 mg / kg group (Res + ATSE100), The mice were divided into 5 groups of a Res + leek seed extract 200 mg / kg group (Res + ATSE200), and water and various aqueous extract solutions were orally administered to each mouse once a day for 1 week continuously. On day 6, 24 hours after administration of Reserpine (1.5 mg / kg), forced swimming was performed for 6 minutes. The cumulative immobility time during this period was measured to evaluate the antipsychotic fatigue effect. The results are shown in FIG.
Furthermore, after measuring the immobility time, the brain was removed from the mouse, the brain was removed from the mouse in the cerebrum, and the neurotransmitter and its metabolite in the cerebrum were analyzed using an HPLC-electrochemical detector. The results are shown in Table 1, and the ratio between the brain neurotransmitter content and the metabolite content is shown in Table 2.

(3) Results According to FIG. 1, when the leek seed extract was administered at a dose of 100 mg / kg or more, the immobility time during forced swimming was shortened as compared with the reserpine administration group. Furthermore, according to Table 1, as a result of comparing the neurotransmitter and metabolite contents in the brain, the NE: norepinephrine content was 7.5 ± 2.5 (ng / g brain weight) in the reserpine administration group. On the other hand, when leek seed extract was administered, it showed an increasing trend of 10.2 ± 3.6 to 20.7 ± 5.7 (ng / g brain weight), and DA: dopamine showed 53. 7 ± 3.0 (ng / g brain weight), when leek seed extract was administered, 55.8 ± 4.3-72.6 ± 10.5 (ng / g brain weight) It showed an increasing trend. Serotonin was 47.8 ± 3.4 (ng / g brain weight) in the reserpine-administered group, whereas 52% when the leek seed extract was administered at 100 to 200 (mg / kg). 7 ± 6.7-55.7 ± 4.4 (ng / g brain weight) showed an increasing tendency. Accordingly, various neurotransmitters decreased by reserpine administration showed an increasing tendency in the leek seed extract administration group. Moreover, as shown in Table 2, as a result of examining the ratio of various neurotransmitters and their metabolites, a recovery action to a normal value (control) was observed in the leek seed extract administration group (Table 2). ). Based on the above, leek seed extract has a mental fatigue recovery effect by relieving mental symptoms in depressed (weakness) state by mental fatigue and restoring various neurotransmitters to normal values. It was recognized that

Test example 2
Measurement of locomotor activity (1) Experimental materials and methods Experimental materials: The above-mentioned leek seed extract and 5-6 week old ddy-dominant mice (23.4-27.6 g) are grouped into 7 groups, 5 groups (control, leek seeds). Experiments were conducted with extracts (Examples) 100, 200, 400 mg / kg, caffeine 100 mg / kg).
Method: The leek seed extract and caffeine were each suspended in distilled water to prepare samples. Orally administered to mice fasted at each dose (100,200 and 400 mg / kg). One hour later, the mouse was placed in a box (22 x 26 cm), taken with a video camera for 3 minutes (1 mouse), and the spontaneous momentum was measured using analysis software. In addition, we evaluated the movement amount (movement distance, cm) and Active time (activity time, sec) as indices. The results are shown in FIG. 2 [Movement Movement (movement distance, cm)] and FIG. 3 [Active time (activity time, sec)].
(2) Results As a result of the experiment, the leek seed extract showed a concentration-dependent increase in Movement (cm) and Active time (sec) in the 100-400 mg / kg group compared to control. (FIGS. 2 and 3). Further, jumping behavior (excitatory action) was observed in the caffeine administration group at the time of measurement, whereas these actions were not observed in the leek seed extract administration group. From these results, it was found that leek seed extract, unlike caffeine, has no excitatory effect and increases spontaneous exercise.

Test example 3
Forced swimming experiment (1) Method
Breeding 35 mice (ddy, male, 6 weeks old) divided into 5 groups: control group, ginseng extract group (100 mg / kg), leek seed extract 50, 100 and 200 mg / kg group, Various aqueous solutions of the extract were orally administered to mice at each dose once a day for 2 weeks. In the first and second weeks, a weight of 10% of the body weight was loaded and a forced swimming experiment was performed (the time until the head was completely submerged in water for 5 seconds was measured). The result is shown in FIG.

(2) Results As shown in FIG. 4, the swimming time duration was increased in the leek seed extract administration group compared to the control group at both the first and second week of administration. . In addition, as a result of comparison with the ginseng extract, which is famous as a nourishing tonic material, it was found that the leek seed extract has a longer swimming duration and is more effective in reducing physical fatigue than the ginseng extract.

Test example 4
Genetic test (1) Method 1. Cell Culture Rat myoblasts (L6) were maintained in DMEM containing 10% FBS.
When L6 was seeded in a 12-well plate and became confluent, the medium was replaced with α-MEM containing 2% FBS, which is a low nutrient medium, and the medium was replaced with a fresh one every 2-3 days. On the 8th day, the sample solution described later was added and further cultured for 1 day.
2. Preparation of sample solution The leek seed extract was prepared in DMSO to be 0.1, 0.3, 1, 3 and 10 mg / mL. 1 μL of this solution was added to each well to make final concentrations of 0.1, 0.3, 1, 3 and 10 μg / mL (1000-fold dilution).
3. DNA sample preparation RNA extraction from cells is performed using the RNeasy micro kit.
(QIAGEN) was used according to the conventional method. RNA was reverse transcribed using random hexamers as primers and SuperScript III reverse transcriptase (Invitrogen) to synthesize complementary DNA. After the reaction, RNA was degraded using Tth RNaseH (TOYOBO).
4). 2. PCR and PCR product evaluation Using the DNA sample synthesized in step 1 as a template, the gene was amplified using primers specific to the target gene. PCR conditions are as follows.
Step 1: 94 ° C, 5 min
Step 2: 94 ° C, 1 min
62 ° C, 0.5 min
72 ° C, 1 min Step 2 × 32 cycles
Step 3: 72 ° C, 10 min

  PCR products were electrophoresed on a 2% agarose gel containing ethidium bromide, images taken with UV light were taken with a digital camera, and the brightness of the band was digitized using NIH image. The results are shown in FIG. 5 (CPT-1B), FIG. 6 (ACOX-1), FIG. 7 (Glycogen Phosphorylase), and FIG. 8 (c-AMP).

(2) Results and Discussion Regarding the anti-fatigue effect of leek seed extract, gene expression of proteins related to energy production was evaluated.
As a result, gene expression of CPT, ACOX related to fatty acid metabolism, glycogen phosphorylase related to glycogen metabolism, and AMPK that regulates metabolism in general was highest at leek seed extract concentration of 0.3 to 1.0 μg / mL. became.
The leek seed extract was found to have an anti-fatigue action due to increased energy metabolism in muscle cells.

Test Example 5
Muscle cell proliferation test (1) Method 1. Preparation of the sample solution The leek seed extract was mixed with 1, 3, 10,
After being dissolved in DMSO at concentrations of 30 and 100 mg / mL, it was prepared in DMEM containing 10% FBS so as to be 1, 3, 10, 30, and 100 μg / mL (diluted 1000 times). 10 μL of this solution was added to each well to make final concentrations of 0.1, 0.3, 1, 3 and 10 μg / mL (further 10-fold dilution).
2. Cell culture Rat myoblasts (L6) were maintained in DMEM containing 10% FBS.
In a 96-well plate, L6 in the logarithmic growth phase was seeded at a concentration of 1.0 × 10 4 cells / mL, and the above sample solution was added. Further culture was performed and evaluation was performed after 2 days.
3. Measuring cell viability
Cell activity or cell number was evaluated by the MTT method. 10 μL of MTT assay reagent was added per well and allowed to act for 30 minutes in a cell culture machine. The yellow color was quantified with a microplate reader with an optical filter of 570 nm, and the control group was standardized as 100%. The result is shown in FIG.
(2) Results According to FIG. 9, cell activity was promoted at a concentration of 0.1 to 10 (mg / mL). Thereby, it was confirmed that it has the effect of reducing muscle fatigue.

Although the compounding example of the composition for anti-fatigue of this invention (leek seed extract) is given to the following, the following compounding example does not limit this invention.
Formulation Example 1: Chewing gum
53.0wt% sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Chive seed extract 0.5
100.0wt%

Formulation Example 2: Gummy
Reduced water tank 40.0wt%
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Kiwi juice 4.0
Kiwi flavor 0.6
Dye 0.02
Leek seed extract 1.0
100.0wt%

Formulation Example 3: Candy
50.0 wt% sugar
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Leek seed extract 0.4
100.0wt%

Formulation Example 4: Yogurt (hard / soft)
Milk 41.5wt%
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Leek seed extract 0.4
Perfume
Water residue
100.0wt%

Formulation Example 5: Soft drink
Fructose glucose liquid sugar 30.0wt%
Emulsifier 0.5
Chive seed extract 0.05
Perfume
Purified water residue
100.0wt%

Formulation Example 6: Soft capsule
Rice germ oil 87.0wt%
Emulsifier 12.0
Leek seed extract 1.0
100.0wt%

Formulation Example 7: Tablet
Lactose 54.0wt%
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Leek seed extract 1.0
100.0wt%

Formulation Example 8: Oral granules (pharmaceuticals)
Leek seed extract 1.0wt%
Lactose 30.0
Cornstarch 60.0
Crystalline cellulose 8.0
Polyvinylpyrrolidone 1.0
100.0wt%

Formulation Example 9: Tablet
76.4 wt% sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Chive seed extract 0.5
Purified water 3.9
100.0wt%

Formulation Example 10: Cosmetic cream
Squalane 20.0wt%
Beeswax 5.0
Refined jojoba oil 5.0
Glycerin 5.0
Glycerol monostearate 2.0
Polyoxyethylene (20) sorbitan-
Monostearate 2.0
Leek seed extract 2.0
Preservative appropriate amount
Perfume
Purified water residue
100.0wt%

Formulation Example 11: Lotion
Ethanol 5.0wt%
Glycerin 2.0
1,3-butylene glycol 2.0
Polyethylene oleyl ether 0.5
Sodium citrate 0.1
Citric acid 0.1
Chive seed extract 0.1
Purified water residue
100.0wt%

Formulation Example 12: Body gel
Macadamia nut oil 2.0wt%
Octyldodecyl myristate 10.0
Methylphenylpolysiloxane 5.0
Behenyl alcohol 3.0
Stearic acid 3.0
Batyl alcohol 1.0
Glyceryl monostearate 1.0
Tetraoleic acid polyoxyethylene sorbit 2.0
Hydrogenated soybean phospholipid 1.0
Ceramide 0.1
Retinol palmitate 0.1
Preservative appropriate amount
Centella extract 1.0
Leek seed extract 1.0
1,3-butylene glycol 5.0
Purified water residue
100.0wt%

Formulation Example 13: Latex
Squalane 4.0wt%
Vaseline 2.5
Cetanol 2.0
Glycerin 2.0
Lipophilic glyceryl monostearate 1.0
Stearic acid 1.0
L-Arginine 1.0
Chive seed extract 0.5
Potassium hydroxide 0.1
Perfume
Purified water residue
100.0wt%

Formulation Example 14: Bath agent (liquid)
Propylene glycol 50.0wt%
Ethanol 20.0
Sodium sulfate 5.0
Chive seed extract 0.5
Lanolin 0.5
Avocado oil 0.5
Dye 1.5
Fragrance 22.0
100.0wt%

  As described above, the present invention can provide a naturally-derived anti-fatigue composition having a function of improving both physical fatigue and mental fatigue. Further, since leek flowers and seeds are used, an anti-fatigue composition that is safe and has few side effects can be provided.

In Test Example 1, the cumulative immobility time was measured to evaluate the antipsychotic fatigue effect. It is a graph which shows the result. It is a graph which shows the effect | action which acts on the spontaneous exercise amount (movement distance) in the mouse | mouth of the leek seed extract of a present Example. It is a graph which shows the effect | action which acts on the locomotor activity (activity time) in the mouse | mouth of a leek seed extract. It is a graph which shows the effect | action which acts on forced swimming of a leek seed extract. It is a graph which shows the expression of the gene of enzyme CPT-1B in a leek seed extract. It is a graph which shows the expression of the gene of enzyme ACOX-1 in a leek seed extract. It is a graph which shows the expression of the gene of an enzyme (Glycogen Phosphorylase) in a leek seed extract. It is a graph which shows the expression of the gene of an enzyme (c-AMP) in a leek seed extract. It is a graph which shows the muscle cell proliferation effect in a leek seed extract.

Claims (7)

At least one of flowers and seeds of leek (Allium tuberosum),
And an extract extracted from at least one of leek flowers and seeds,
An anti-fatigue composition comprising at least one of them as an active ingredient.   The anti-fatigue composition according to claim 1, wherein the leek seed is defatted. At least one of ginseng, garlic, sorghum, turmeric, rahan fruit, kava hippo, kankanikuyu and licorice,
And an extract extracted from at least one of ginseng, garlic, sorghum, turmeric, rakan fruit, birch hippo, kankanikuyu and licorice,
The anti-fatigue composition of Claim 1 or Claim 2 which further contains at least 1 sort (s) of these as an active ingredient.   The anti-fatigue composition according to any one of claims 1 to 3, further comprising γ-aminobutyric acid and / or a derivative thereof as an active ingredient.   A pharmaceutical comprising the anti-fatigue composition according to any one of claims 1 to 4.   A food and drink comprising the anti-fatigue composition according to any one of claims 1 to 4. An external preparation for skin comprising the anti-fatigue composition according to any one of claims 1 to 4.

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