TW200827307A - The treating method of aquaculture waste-water and the apparatus thereof - Google Patents

Abstract

The present invention relates to the treating method of aquaculture waste-water, which includes: providing an aquaculture tank, an aeration-typed waste water-treating tank connecting to aquaculture tank, a pump connecting to aquaculture tank and aeration-typed waste water-treating tank, and a lighting fixture; placing the alga pre-treated by fixation into the aeration-typed waste water-treating tank; starting the pump to make culture media in aquaculture tank flow into the aeration-typed waste water-treating tank; then removing out the nitrides in the culture media; and finally flowing back to the aquaculture tank. This invention also provides the apparatus relating to aforementioned method.

Description

200827307 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a method for treating aquaculture wastewater and a device for use in the above method. [Prior Art] When sewage treatment, it can be divided into physical, chemical and biological treatment methods. At present, most of the domestic circulating aquaculture adopts biological treatment method, in which the biological Xinxin bed has achieved good results in application. The stone-sharpening treatment is often accompanied by problems such as the addition of the test, the backwashing of the coffin and the accumulation of acid and nitrogen. Therefore, there is still the treatment of sewage by algae, because algae can absorb and absorb nitrogen compounds such as ammonia nitrogen, nitrite nitrogen and nitrate nitrogen as nutrient sources, and can also provide dissolved oxygen in water during photosynthesis. To achieve the effect of improving water quality (Ye et al., 1996). At present, the commercial application of algae treatment wastewater only has an oxidation pond and a high rate of 10 algae 'equation'. The design of the towel oxidation pond is to maintain the pond aerobic state, using aerobic bacteria to decompose organic matter in the wastewater, and algae The role is to remove B0D and inorganic nutrients (Wu et al., 1 992); the high rate algae pool is to obtain a large amount of algae, and can remove B〇D and nutrient salts (〇3仙1 (1,1978). Although oxidized Ponds and high-rate algae ponds have the advantage of being economically effective, but there are also problems that are susceptible to environmental changes and are not easy to maintain the separation of single dominant algae species and algae from the runoff water (Lu et al., 19^3); and the above-mentioned oxidation ponds and When high-altitude algae ponds are used in the treatment of circulating wastewater, most of them do not use immobilization technology to fix algae, and the related use of immobilized 200827307 technology is still mainly based on the related basic tests of subtractive or continuous non-recycling. In order to overcome the above problems, the immobilization technology is currently used, so that the microorganisms of the solid sputum are easily separated from the discharged water, the microorganisms are not easily lost, and the foreign matter 〖the biomass is less harmful to the microorganisms, and no regular backwash is required. The treatment efficiency is improved and the microbial selection can be different depending on the nature of the wastewater, and the research on the fixed treatment wastewater has been paid more and more attention. The single cell microalgae is used for immobilization to treat various wastewaters, such as removing nitrogen and phosphorus nutrients. There are also a number of related studies published to remove nitrates and remove urea (Chevalier and de la Notie, 1985; Garbisu et al., 1991; Mak and Trevan, 1988; Tam and Wong, 2000), however The literature on the application of immobilized algae to aquaculture is still relatively small, and most of them are only for “batch type, ammonia nitrogen removal of fish excreta during aquaculture, and the practical application in the study of circulating water systems is as follows. SUMMARY OF THE INVENTION In view of practical application in the research of circulating water system, the present invention further utilizes the immobilization technology to fix the algae and then cooperate with the use of the circulating water system to further achieve the purpose of treating the aquaculture wastewater. The present invention provides a The treatment method of the aquaculture wastewater includes: providing a culture tank and an aerated wastewater treatment connected with the culture tank a pumping motor connected to the aquaculture tank and the aeration-type wastewater treatment tank and a lighting device disposed around the aeration-type wastewater treatment tank; 6 200827307, the immobilized algae and the like are placed in the exposure The gas-type wastewater treatment tank opens the pumping motor, and the nutrient solution of the raid-culture tank can be flowed to the aeration-type wastewater treatment tank for removal of the nitrogen compound, and then returned to the culture tank. Preferably, the algae is a green algae. Preferably, the green algae is chlorella. Preferably, the chlorella concentration is ΙΟΟχΙΟ4 to 4〇〇〇χ1〇4 cells/ml. More preferably, the The chlorella concentration is 12〇〇xl〇4ceUs/ini. Preferably, the illuminating device is continuously irradiated in the irrigator type wastewater treatment tank. Preferably, the illuminating device is disposed on both sides of the irradiant type wastewater treatment tank. Preferably, the illuminating intensity of the illuminating device is For i, 〇〇〇 to 15, OOOlux. More preferably, the illumination device has an illumination intensity of 7,7 lux. Preferably, the hydraulic residence time (HRT) of the algae bead is set to 1 to 6 hours. More preferably, the Hydraui ic Residence Time (HRT) of the steam bead is set to 2 hours. In another aspect, the present invention provides a treatment device for aquaculture wastewater, comprising: a culture tank for providing biological growth and having a water outlet and water inlet v; an aerated wastewater treatment tank connected to the culture tank; Providing a plurality of 200827307 algae placements and forming a water inlet and a water outlet respectively connected to the water outlet of the culture tank and the water inlet, and two adjacent tank chambers are formed in the aeration wastewater treatment tank, one of which Providing an algae bead and a buffer tank for placing the algae, and the water inlet is disposed on the groove wall of the algae groove, the water outlet is located on the groove wall of the buffer tank, so that the culture liquid can pass through The algae bead is followed by the buffer tank; a pumping motor connected to the aquaculture tank and the aerated wastewater treatment tank is taken out of the culture tank in the culture tank and then enters the aeration wastewater 10 treatment tank; And an illumination device disposed around the aeration-type wastewater treatment tank is irradiated on the aeration-type wastewater treatment tank. Preferably, the opening of the top end of the aerated wastewater treatment tank is further provided with a barrier network which prevents debris from falling into the algae channel and also prevents the algae from flowing out of the algae. Preferably, the barrier network laid on the algae groove is a nylon mesh. _ Preferably, the aerated wastewater treatment tank is placed above the culture, so that the water level in the aeration wastewater treatment tank is higher than the culture tank, and the culture liquid in the buffer tank can be Return to the culture tank. Preferably, the pumping motor is placed in the culture tank as a submersible motor. 9 Thereby, the invention utilizes the technology of immobilized algae and the circulation design of the mixed culture wastewater, and can continuously treat the aquaculture wastewater to achieve the purpose of changing the water quality, and further improve the problems encountered in the previous use of the nitrification filter bed. · question. . 8 200827307 [Embodiment] The term "immobilization treatment" as used in the present invention refers to the use of chemical or physical means to restrict the movement of cells or to block cells in a certain space, and to allow repeated use of the cells, and The physiological activity of the cells is not affected by immobilization. Among them, the immobilization method includes a microcapsule method, an attachment method, a cross-linking method, an aggregation method (Aggregati〇n), and an encapsulation method. In the present invention, the cells are coated on the alginate by a colloidal coating method, but the immobilization treatment of the present invention should not be limited to the above. Hydraulic retention time (Hydraulic)

Residence Time, ΜΊ), total 4 miles! —〆 ” means the time at which the wastewater stays in the aeration wastewater treatment tank. In the present invention, the flow rate of the culture fluid is controlled by the motor to control the time during which the culture fluid stays in the aeration wastewater treatment tank. Production m,: a culture tank, an aerated wastewater connected to the culture tank: a pumping motor in the aquaculture tank and the aeration wastewater treatment tank and a lighting device disposed around the aeration wastewater treatment tank The algae is treated in the aerated wastewater treatment tank; and after the removal of the gas compound, it is returned to the culture tank.) (5)": The solid algae can effectively remove the circulatory aquaculture system. (ΝΗ4) and the removal efficiency of nitrite are about 6 2 (: 乂 ', which is _ and 48.29 hearts and 6 / ° in the actual aquaculture test, and the average removal rate is about ay, respectively, the present invention is actually The application is applied to the 9200827307 colonization system, and the treated wastewater will be recycled and reused, and an aeration device is installed in the immobilized algae pool, and a nylon barrier mesh is placed on the top end thereof to avoid the immobilized algae beads overflowing. The other features and advantages of the invention will be apparent from the following description of the preferred embodiments of the invention. Show how to implement the materials and methods of the present invention. Example 1: Algae cultivation and fixation 1 · 1 algae Source: Chlorella (sp·), provided by Dr. Su Huimei, Biotechnology Group of the Water Test Institute. 1 · 2 algae cultivation : Cultured in Bri s to 1 medium, the ingredients are shown in Table 1. Table 1 Bristol culture formula (Stein, 1973) 940 ml of sterile deionized water was added to the following concentrate 1 〇mi

NaN03 10 g/400 ml CaCl2 · 2H20 1 g/400 ml MgS04 · 7H20 3 g/400 ml k2hpo4 3 g/400 ml kh2po4 7 g/400 ml NaCl 1 g/400 ml 1 · 3 Immobilized algae beads: Prepare 1% (w/v) 1 - liter of Na-alginate solution, and sterilize in an autoclave at 12 rc for 30 minutes. After cooling, add an equal volume of chlorella solution to the alginate solution. That is, the chlorella liquid is more ««200827307 1 : 1 than the brown algae liquid and stirred by a magnet stirrer (Fargo MS-90) for about 10 minutes to make it evenly mixed. Pour the algae solution containing chlorella into a 50 ml burette and titrate into a 5% calcium chloride (CaCl2) liquid. Each milliliter of alginate mixture can form 20 alginate beads, each of which has an algae. The bead diameter was about 5 mm, and the formed algae beads were allowed to stand for 12 hours and then rinsed with distilled water to remove residual calcium chloride solution. Then fix 2 liters to 40,000 algae beads and separately sterilized 1 liter of 1 〇 concentration synthetic waste liquid, 〇 2 liters of nitrite ion (N02-) stock solution, 2 liters of nitrate ions (NO 〆 The stock solution and 6.8 liters of deionized water are added to a 15 liter gram cylinder and cultured in a constant temperature and humidity chamber (KATO SE-23ECN) with a light intensity of about 4300 lux and a temperature of 25 °C. It is pumped with a two-hole aerator (forever 3^800〇). The aeration volume is 3〇1/111111 per well. For the 48 hours to be cultured, ammonium (NH4+) and nitrite ions (N) in the artificial waste liquid. 〇2-) and nitrate ions (NOf) are removed to obtain a high concentration of chlorella algae beads. Example 2: Batch nitrogen compound removal test 2.1 Water quality analysis items and methods Temperature, dissolved oxygen (DO), pH, ammonium (leg 4 + ), nitrite ion (n〇2_) = nitrate ion (Ν〇Γ) concentration, the various measurement methods are shown in Table 1. = Measured data will be further analyzed statistically to understand the effect of chlorella beads under various factors on their removal of nitrogen compounds. 11 200827307 Table 1 Water Quality Measurement Method Table Project Measurement Instrument Brand Measurement Method Temperature Dissolved Oxygen Sensor Temperature Skin Measurement Function WTW Direct Measurement Dissolved Oxygen Dissolved Oxygen Sensor WTW Direct Measurement pH Acid Tester SUNTEXTX-2 Direct Measurement NH4+ Concentration Spectrophotometer Spectronic 20 GENESYS Indigo Method N〇2_Concentration Spectrophotometer Spectronic 20 GENESYS Spectrophotometer N〇3-Concentration Spectrophotometer Spectronic 20 GENESYS Horse Money Sub-method 2 · 2 ammonium (NH4 + ) removal test 2 · 2 · 1 adsorption test will be algae-free beads in synthetic waste liquid (components shown in Table 2), and algae-free beads and chlorella The algae beads were tested in the ammonia-nitrogen solution containing only ammonium (NH4+), and the adsorption of the beads and the ammonium removal (NH4+) test of the algae beads were carried out to investigate the adsorption effects of different test solutions on the beads. The results showed that the algae-free beads were The artificially synthesized waste liquid ammonium removal (NH4+) test was tested for 36 hours. The ammonium (NH4+) in the test solution was completely removed by the algae-free beads. When the ammonia nitrogen stock solution was added at the 48th hour, the beads were in the 96th hour. Hardly after Further, the ammonium (NH/) is adsorbed, and the ammonium (NH/) saturated concentration is about 38. 65 rag/1, and the average removal rate of the ammonium (Nil) solution is about 17.93 mg/1. · day, algae-free beads and algae-containing algae beads in ammonium (NH4+)-containing ammonia nitrogen solution, the test results of ammonium (NH4+) concentration changes similar to the initial 24 hours of the test, 12 200827307 but NH4) removal is slower than the synthetic waste liquid group, and its removal efficiency is only about 10%, while the synthetic waste liquid group is about 6〇%. It also shows that the trace elements in the waste liquid of human workers can promote the adsorption of ammonium by the beads. The role of (NH4 + ). Table 1 Synthetic Waste Liquid Formula (Tam and Wong, 2000)

Bacto-peptone 58.9 ms/1 Glucose 225.0 mg/1 (NH4)2so4 141.3 〇mg/1 k2hpo4 33.7 mg/1 MgS04 · 7H20 50.0 mg/1 MnS04 · H20 5.0 mg/1 FeS04 · 7H20 2.2 mg/1 KC1 7.0 mg /1 CaCl2 3.7 mg/1 NaHC03 277.7 mg/1 2 · 2 · 2 Photoperiod test Photoperiod test was carried out with chlorella cultured in green algae culture solution (components as shown in Table 3) (Su, 1 999) After the beads were immobilized, the algae bead batch type ammonium removal (NH4+) test was carried out by continuous operation and 12/12 hour intermittent illumination 'two sets of light conditions. The test period was 48 hours to analyze and compare the photoperiod to the algae beads. The growth of algae cells and the effect of algae beads on the removal of ammonium (NH/), pH and dissolved oxygen (D0) in the synthetic waste liquid showed that the initial algal cell concentration of the algae cultured in the green algae culture solution was about For 226^104 cel ls/ml, the concentration of chlorella in the prepared algae solution is about 113x104 cells/ml, that is, the chlorella contained in each algae is about 5.65χ1〇4 cel Is, and continuous The initial ammonium (NH4+) concentrations of the synthetic waste liquids in the intermittent illumination group were 25.66 and 26·16 mg/1, respectively. It can be found that the concentration of chlorella in the intermittent light group was slightly higher than that in the continuous illumination group at the 24th hour, and the continuous illumination group after 48 hours of experiment, the chlorella proliferation 13 200827307 was slightly higher than the intermittent illumination due to the effect of continuous photosynthesis. The concentration of the two groups was about 742χΐ〇4 and 597XW cells/ml respectively, that is, the chlorella contained in each algae was about 29.85xl〇4 ceUs cells, respectively. In addition, the ammonium (NH/) concentrations of the continuous and intermittent illumination groups were completely removed within 48 hours, and the average removal rates were 12.83 and 13.08 mg/1/day, respectively, reaching the 24th and hourly groups of ammonium (NH/ The concentration is reduced by 2〇3〇 and 1611丨, respectively, and the value can be found to decrease first and then rise again. The phenomenon of straight _ can be absorbed by 兮α 八 phenomenon, which is also the rate of algae ingestion (ΝΗ/). For photosynthesis, it is necessary to use 夤吟 夤吟 乳化 乳化 乳化 , , , , , , , , , , , 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化 乳化_4·4 / 丨 社 社 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 且 缤 缤 缤 缤 缤 缤 缤 缤 光照 光照 光照 光照 光照 光照 光照 光照 光照 光照 光照 光照The concentration of chlorella in the continuous illumination test group was higher, so the photosynthesis was more vigorous, resulting in more oxygen generation, but the effect was not significant. The dissolved oxygen content of the two groups (D〇 distribution was also mainly affected by Effect of aeration. Table 2 Green algae culture solution formula (Su, 1999) 2 · 2 · 3 different concentration test This test is green After the algae cultured in the algae culture solution was fixed by algae beads, the artificial synthesis waste liquid with ammonium (〇4+) concentration of about π, = and 35 mg/1 was set up in groups A, B and C respectively. The test was conducted after the three groups of initial ammonium (NH,) content were completely removed.

After the biting hours, and the subsequent test phase after JL, the ammonium (NH/) in the test solution, < Bali is almost completely removed from 200827307, adding a new ammonia nitrogen reserve solution to remove the ammonium from the transport + continuous performance 4+) test,

In order to observe the ammonium removal (nh4+) ί 月 shape of the solidified algae beads under the three kinds of ammonium (leg 4+), the group A is in the 96th, 120th, m 〇Ίβ, ^ Buxin 144, 216 and 264 hours to add a new ammonia nitrogen stock solution, so that the concentration of ammonium (ΝΗ4+) in the waste of the workers' workers is restored to about 12.96, 13, 00, 13 Sichuan 1 / f 0 〇 ^ D 1 < 5 · 29,14·32 and 14·12 mg/l; β group in the 96 and 216 hours of Heshi* addition of new cyanide reserve solution, the concentration was 21.96 and 24·63 mg/i • Γ , μ ^ i, while in group C, new ammonia nitrogen stock solution was added at 96 and 240 hours. The potted total/length was 31·54 and 32.84 mg/Ι, and all three tests were also tested.曰 曰 忒 忒 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 192 ^ 2 Ρ 之 5, Ό 显 , 当 当 当 当 当 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工 人工Prepared The concentration of chlorella in the brown algae solution is about 51xi〇4ceiis/mi, that is, the steam contained in each steamed bead is about 2 55xlQ4 ceUs, the A group is at (10) hours, the B and G two (four) are at 144 small materials. The highest growth concentration of the material was 255〇χ104, 298〇χΐ〇4 盥%9 iU Xing Zb2〇xl〇4 cells/ml, and it showed a downward trend after the highest value. / Chen, the reason for the decline may be that the trace element in the synthetic waste liquid is not supplemented with hibiscus, resulting in a slow rate of ammonium removal (NH4+), resulting in insufficient energy to provide growth and growth of Chlorella in the algae.

: Outside 'when the test reached 36 hours, group A's Qing h4+) was completely removed, and B. The ammonium _,) of the two groups were also completely removed at the 42nd hour, and the three groups were in this initial ammonium removal (NIV) stage, reaching the average of the total removal of 15 200827307 with the increase of the initial ammonium (NHV) concentration. The phenomena of improvement are 9.93, 15·37, 20·41 mg/l/day, and all have the effect of stable ammonium removal (NH4+), 'A group is added for 144 hours when the test is 144 hours. After the ammonia-nitrogen reserve solution, the semi-continuous cycle of ammonium removal (NH,) stage, the rate of ammonium removal (NH4+) of algae beads slowed down. This situation also appeared in the two test groups of g and C, which may be due to synthetic waste. The trace elements in the liquid which promote the adsorption and uptake of the algae beads are insufficiently consumed, and the adsorption test described above is consistent with the slow effect of the removal of ammonium (νη4+) by the algae beads without the supply of trace elements. 10 2 · 2 · 4 nitrite ion (ν〇2-) removal test This test first prepares 980 ml of sterile synthetic waste liquid without added (NH4)2S〇4 and adds nitrite nitrogen stock solution 2〇, This is the synthetic waste liquid required for the test. The test was carried out by immobilizing algae beads with chlorella cultured in Brist〇1 culture medium for a test period of 48 hours. The results showed that the initial algal cell length was about 182×10 4 cel ls/ml, which was small in the prepared algae solution. When the concentration of chlorella is about 91x104 cells/ml, the growth concentration of chlorella in the algae beads is about 347><1〇4 cel is/mi at the end of the test, indicating that chlorella can also take up nitrous acid in the algae beads. Root ion (Ν〇Γ) to proliferate; while observing the concentration change of nitrite ion (NOf), the initial nitrite ion (N〇2) concentration was found to be about 17.8〇mg/1, which was tested for 24 hours. When 9 (10) or more were removed by algae beads, the concentration of nitrite ions (n〇2_) removed was about 16·13 mg/i, and it was completely removed after 48 hours of the test, indicating that the ball was also well removed. The capacity of the nitrate ion (N0 plant); in addition, the pH range of the test is between 8.27-8·9〇, and the pH value drops first at the beginning, which may be due to the aerator aeration. The carbon dioxide produced is 16 200827307; the dissolved oxygen (DO) varies between 4 and 4 3 mg/1

2 · 2.5 flux removal (N〇3-) removal test This test also prepares 800 ml of sterile synthetic waste liquid without addition (Off 4) Ah and then adds nitrate nitrate reserve solution 2, ie In order to test the synthetic waste liquid required for the test, the test was carried out by immobilizing the algae beads with the chlorella cultured in the Bristol culture solution, and the test week (four) was 72 hours. The results showed that the initial algae cell concentration was about I 88xlG4 cells/ml 'the brown turn The concentration of chlorella in the liquid was about A 44xl 〇 4 cells/ml. The initial acid ion ion (xian 3-) concentration of the test was about 28.44 鞑 / 1. The growth concentration of the small vine was at the 72nd hour of the test. Up to about 850x1〇4 cells/ml, it is known that nitrate ion (10)3-) is also a good nitrogen source for providing chlorella; in addition, 'observing the acid ion (Ν0Π, the test was about 92% removed at the 48th hour, and Complete removal in the 72nd hour, and the average removal rate in the first 48 hours is about y / 1 · day; in addition, the pH value will decrease first in the initial stage, which may be caused by the carbon dioxide generated by the aerator; The range of the amount varies between 4. 0-4. 3 mg / Ι, and does not accompany the algae The cell concentration increases and rises. Example 3: Circulating aquaculture system Please refer to the first figure. The recirculating aquaculture system used in the present invention includes - (4) (1 〇), - and (4) connected aeration. a wastewater treatment tank (20), a pumping motor (3〇) connected to the aquaculture tank (1〇) and the aeration water treatment tank (20), and a periphery of the exposure type wastewater treatment tank (20) Illumination device (4〇), wherein the j culture tank (1 G) provides biological growth and has a water outlet and an inlet, and the aeration wastewater treatment tank (2〇) is placed higher than the culture ^ 17 200827307

(1 〇), and the aeration wastewater treatment tank (2 〇) also forms a water inlet and a water outlet respectively connected to the water outlet of the culture tank and the water inlet 'the aeration wastewater treatment tank (20 Further, an algae bead (21) and a buffer tank (22) for providing algae placement are formed, and the water inlet is provided on the groove wall of the algae bead (2 1 ), and the outlet of the algae is located a groove wall of the buffer tank (22), and a barrier net at the top opening of the algae bead groove (2 1), the barrier net prevents the debris from falling into the algae groove (21) The algae beads are also prevented from flowing out of the algae bead tank (21). In use, the pumping motor (30) is used to extract the culture liquid in the culture tank (1) and then enter the aeration wastewater treatment tank. The nitrogen compound is removed from the algae groove (2 1 ) of (2 〇), and then the culture solution flows into the buffer tank (2 2 ), and finally the position of the aerated wastewater treatment tank (2 〇) is higher than the The culture tank (1 〇) is caused to generate a water level difference, and the nitrogen-removed culture liquid in the buffer tank (22) is returned to the culture tank (1〇) to complete the cycle. The following is a detailed description of the embodiments of the present invention, but it is not necessary to limit the invention. The length, width and height of the culture tank (1 〇) are respectively? 80, 56 〇 and 480 mm, and a transparent acrylic container with a thickness of 5 mm, and the length, width and height of the aerated wastewater treatment tank (20) are 300, 200 and 200 mm, respectively, and 150 mm south Separating into an algae groove (2 1) and a buffer tank (22) with an acrylic plate having a width of 200 mm, wherein the length, width and height of the algae groove (21) are 200, 200 and 150 mm, respectively, and the aeration The bottom of the tank of the wastewater treatment tank (2 〇) is about 62 cm higher than the bottom of the tank (1 〇), and about 20 mm on both sides of the algae groove, one 20 W fluorescent lamp is placed respectively (East Asia FL 20D-EX/18), whose light intensity is approximately 77 GG lux' measured by a digital illuminometer (TECPLE 18 200827307 530) as a light source required for chlorella in the algae beads. ^ The pumping motor (3 〇) is preferably a submersible motor (Ri〇6〇〇) and is disposed in the culture tank, and the culture liquid in the culture #(1Q) is pumped to the medicine bead tank (1)) After the treatment, the effect of the water level difference is used to flow back to the culture tank (10). In addition, since the barrier network is covered on the algae groove, the algae beads can be prevented from being in the algae groove (4), preferably The barrier mesh is a nylon mesh. The above-mentioned breeding tank (1 〇) has an effective water body of about 100 liters, and the algae sump (2 1 ) treats wastewater with an effective water body of about 6 liters. During the test, the culture tank (丄〇) and the algae bead tank (2 ι) were pumped with 2 and 1 double-hole aerators (forever SF8〇〇〇) to provide the oxygen and algae required by the Wu Guoyu. #中小玉求藻的胄的焦碳. 3 1 Nitrogen compound removal test of aerated algae channel is to provide algae beads required for aeration algae tank. This test uses 1 liter of chlorella solution cultured in Brist〇i culture medium and 1 liter of sterilized After 4% (w/v) alginate solution was uniformly mixed, pour into a 50 ral burette and titrate into a concentration of 5% calcium carbonate liquid to form alginate beads; the formed algae beads were allowed to stand for 12 hours before being treated with distilled water. Rinse to remove residual calcium chloride solution, and then fix 2 liters to 40,000 algae beads and separately sterilized j liters of 1 〇 concentration synthetic waste liquid, 0.2 liters of nitrite ion (N 〇 factory) reserve Solution, 2 liters of nitrate ions (Ν〇Π stock solution and 6.8 liters of deionized water together with a 15 liter acrylic barrel placed at a constant temperature of about 43 lux and a constant temperature of 25 ° C The culture is carried out in a wet tank (KATG SE-23ECN) and is aerated with a two-hole aerator (Always SF-8000) with an aeration of 3·0 Ι/min per well. Medium ammonium (NH^), sub-200827307 to obtain high nitrate ions (N〇2_) and nitrate ions (N〇1 2-) Thereafter, the concentration of the chlorella of the chlorella is placed in the algae groove (2丄). The test system of the present invention sets the 7 (Hydraulic Residence Time, hrt) of the algae groove (2 i ) to 1 hydraulic retention time. Good time...hours, continuous light and aeration, to 6 hours ' 〇) also tested under the same operating conditions of continuous aeration tank (1 bead group as a control group. In actual weight, all weight About and with the algae-free Wu Guoyu as the experimental breeding organism, during the test period, 8 〇1: 〇° in the morning was divided into two averages, and the daily vote was 4g, · Ben: ΓΓΓ; Τ afternoon Starting at 5:°°, in addition to the simulated water culture sampling and the concentration of chlorella in the algae beads; and the others, the rest have the actual aquaculture experiment of stocking Wu Guoyu, i water quality is not 48 hours before each test. 6 hours, the sampling position is in the = measuring tank located near the inlet and outlet and the center, and the sampling depth is about 10 cm, and the -#遂曰, w is in the water and the second is repeated. Summer test, and the three measurements are less averaged for analysis, in addition, the aeration The wastewater treatment tank is sampled, and the sampling position is located at the influent σ and the effluent σ and 0) the steam bead tank (21) and the buffer tank (22), and the water inlet is in the form of The water motor pumping did not enter the algae channel (: 20 3 ) 1 · 2 Results 2 The artificial waste liquid of the algae beads before the test of the algae group in the actual culture test, 3) the sample before the sample, and the sampling method of the water outlet was in the algae Slot (2 !) ψ 4 The culture solution flows to the vicinity of the buffer tank (2 2 ). 5 The initial ammonium (ΜΗ/), nitrite ion (Ν〇2_) and nitrate ion (Ν〇200827307 concentration respectively About 18.52, 20.12 and 18.03 mg/i, and the concentration of immobilized chlorella in the algae group during the actual culture test was about 262x1 〇4, 133〇χ1 〇4 at the initial test, 120 and 312 hours respectively. With 143〇xl〇4 cells/ml, the results showed that the excrement and residual bait of Wu Guoyu were decomposed during the actual breeding, which resulted in sufficient supply of nutrient source for circulating wastewater, and the concentration of chlorella showed an upward trend.

The change of ammonium (nH4+) concentration in the initial stage of the algae group and the control group was caused by the decomposition of the excrement and residual bait continuously discharged by the Wu nationality fish, and the consumption of chlorella in the algae beads was small, resulting in Its concentration rises and then falls at the beginning of the experiment' and it is also affected by the decomposition of nitrite bacteria in the culture solution and the uptake of chlorella. The overall change is also the highest in the cultivation, and the buffer is the second in the inlet of the vine bead. The outlet of the tank is the lowest (as shown in the second figure); the concentration of ammonium (10)4+) in the inlet of the inlet and the outlet of the buffer tank is respectively at the peak of the 96th, 96th and 6th, and the peak value is 2 H. 99 L1 二Γ' and the buffer tank outlet also tends to zero value at the end of the test, and the value of the tank inlet is also very low, between .15_ • G Bu G·12 mg/1; Please refer to the third figure. The rise and fall of the human body also rises and then decreases similarly to the steamed bead group, but in the absence of chlorella uptake, the minimum ammonium (nh4+) concentration is present, and ammonium: 7 mouths were not tested for vomiting, and 2 - the sputum (NH4) concentration was reached at the 96th hour of the test. Shang's are about 4 24, 3. respectively. At the same time, there is no role of small ball vines. 19 tons / 1, Γ white 冋 in the side of the bead group culture tank, Luojio tank water inlet and buffer tank outlet three curved algae Beads ^ Ab. The highest degree of 'accumulation of the algae bead placement effectively suppresses the increase in money in the system," and it is also affected by the presence of nitrite bacteria in the system after the test 21 200827307, and tends to be completely decomposed. It is removed, so that when the low-density stocking of the squid, the nitrite bacteria in the culture solution have the effect of decomposing ammonium (NH/) without causing a cumulative increase. Further, the algae group has no The change of nitrite ion concentration in the algae beads group was higher than the nitrite ion inlet (n〇2_) at the water outlet of the algae bead tank 30 hours before the test, and the buffer tank was discharged after the 36th hour. The W-acid ion of the nozzle (N(V) is lower than the inlet, indicating that the ability of the algae to remove the nitrite ion (N〇2-) has been higher than that of the ammonium (NH4+) to produce the nitrite ion (Ν〇Γ). When the initial ammonium (NV) concentration of the culture solution is low, the algae produces amnesium during ammonium (NH^) removal. The time of the root ion (N(V) will be shortened. #第图图, the figure also shows the nitrite ion (Ν〇Γ) of the algae bead culture tank, the algae bead inlet and the buffer tank outlet. The concentrations reached j high at 192, 192 and 216 hours, respectively, and their values were about η·31, 1148 and i〇39 mg/i, respectively, and the lowest value of the algae channel outlet, and the test reached the 312 hours. The concentration of sputum decreased to about 4·11, 4· 〇4 and 3.76 mg/b, respectively. The reason is mainly due to the combination of algae ingestion and decomposition of cinnamic acid bacteria. As shown in the figure, the initial stage of the control group is in phase with the algae group. (4) There is no small (four) metabolites in the bead tank and the decomposition of nitric acid bacteria causes the concentration of nitrite ions (10)2_) in the outlet to be higher. The water, and the water inlet of the culture tank, the algae bead inlet and the buffer tank are all at the first 144 hours, the highest concentration is about 14, 89, 15·01, and there is no effective inhibition of algae ingestion. The nitrite is separated from the 2) formation, which is higher than the culture tank of the algae bead group, the algae tank inlet 22 200827307 and the buffer tank effluent The highest indifference, at the same time, the highest concentration of nitrite ion (N〇2—) in the t-culture medium, and also due to the proliferation of nitric acid bacteria in the culture solution, began to decline and tend to be completely removed. Therefore, it can be seen that the nitric acid bacteria in the culture medium when the low-degree stocking of the Wuguo fish is also like the action of nitrite bacteria on ammonium (NH4+), which can decompose the nitrite ion (no2-) without causing it to Continue to accumulate in the system.

Furthermore, the changes of nitrate ion (N〇3_) concentration in the algae group and the control group are as follows: Fig. 7 and Fig. 7 show that both the culture tank, the algae tank inlet and the buffer tank π are both The phenomenon of continuous and gradual rise appears. The sixth figure shows that before the test f U4 hours, the steam outlet of the steam bead has a higher value, and the original = is due to the metabolic influence of the uptake of ammonium and the acidification of chlorella by the chlorella in the algae beads, and When there is nitric acid in the culture solution, it is also caused by: to the small ball. The algae ingested nitrate 'acid ion (N(V) is more alkaline (〇4+) and nitrite away (N0j)k, which leads to the effect of algae removal of nitrate ion (N〇3_). Large 'culture tank, algae tank inlet and buffer tank outlet ^ test 312 hours of concentration respectively (four) 14 (10), 15 shoulders and 15 98: ϋ shows no algae beads group of nitrate ions (N (v) concentration, test = The water outlet of the buffer tank is the highest, followed by the inlet of the bead tank, and the lowest in the raising tank, the display of the (four) and algae channels are rich in bacteria, and the water in the full bead tank is π and the buffer tank outlet is At the 312th hour of the test, the concentration of acid ion (10)3_) increased to about 1? 8 η." and ^«ng/b· are higher than those with algae beads, and the algae can also have root ions. (no3-) the effect of increasing the concentration. Further calculation of ammonium (NH,) and nitrite ions (Ν〇2·) to go 23 200827307 In addition to the efficiency 'obtained as shown in Table 4 removal efficiency (% Bu [( algae groove :二下户;不: 卫禅", tg/day) = [(the concentration of the algae groove inlet) - (edge two real two agro-degree)] x (algae per tank treatment water volume) Time'

48 72 6 6 65 12 The removal of the (4) bead (NH4+) in the table: ":::? * Removal efficiency = 12% of the group; the ytterbium ion (10) of the two groups, the rate is only two The removal efficiency is low, and the average removal efficiency of the metabolic 1', 8%' algae group is due to the removal of ammonium (NH4+) process (: ^ select J nitrate ion (N02) removal rate, according to the above public The average removal rate of ammonium (NH/) in the algae group and the non-algae group was 24 200827307: 26.61 and o' mg/day, respectively, and the algae group had a higher value and there was a larger unit time. (H+) is removed; the average removal rate of the arsenate ion (rib 2_) is about 48.29 and 22.89, respectively, and it is also shown that the effect of algae beads can result in a larger removal rate, that is, in unit time. The nitrite ion (Ν〇Γ) is removed. The value of the Η value of the water inlet of the algae bead, the water inlet of the algae and the outlet of the buffer tank, the measurement results are respectively 7.99-8 47, 8.27-86 61 between U6-8.55 'the average value is 8 32 8 η and 845:,

^The π ρ Η value of the vines in the vine bead is lower than the inlet; while the Qi value of the water channel of the control group, the water inlet of the ball and the outlet of the buffer tank are respectively [I is "78 2: 8 55 and 8.18 Between -8.55, and the average value of Γη:: and 8.42' respectively, and the pH value of the water inlet of the algae bead tank and the water outlet of the buffer tank is 4, indicating that the steam-free bead without algae beads has a weaker effect. 9 with = the water quality depreciation of the two groups are all suitable for breeding purposes... y· υ 围 围. In addition, the dissolved oxygen content (DO) of the steaming bead culture tank, the medicine bead tank inlet and the buffer tank outlet is between 4.4_5 〇 and 4 3 5 θ outlets ^ ^ 4.3-5.0 mg/1, respectively. The average dissolved oxygen (10)) is about $ 4 in the control culture tank, the inlet of the vine bead and the g, and their distribution is between (1).4, 4.8, 5.=\?? The oxygen content (10) is about 5 ι, 5·3 and ... respectively. The test results show that they are affected by aeration, and the dissolved oxygen content of both is the same: the phenomenon of stable distribution, and (4) the dissolved oxygen of the group of algae beads Increase, may be the number of algae beads in the test), ..., Mingli is still not enough, its photosynthesis is still 25 200827307 is not enough to affect the dissolved oxygen content of algae beads (10). _ #者'Algae group culture tank, algae bead tank inlet and buffer tank outlet change again'1 test results are between 23.2-27. 7, 24. 3-28 4 ^ 24 · 3; 28 · ^ ^^ 25 ^2;>5 ', J6.4 C 'Control culture tank, algae tank inlet and buffer tank outlet 1 degree 77 other than 20.3-25.9, 20. 5-26· 8 20. 6-27. Between 0 °c 'the average temperature is about 22.9, 23.5 and 23. 6 ° C; two, and the test also shows that the algae groove is affected by light and has a higher temperature At the same time, both groups were also affected by climate change, resulting in the temperature of the algae-free group was lower than that of the algae group. This also led to a higher tendency of dissolved oxygen distribution. During the test period, it was also found that the dissolved oxygen in the systems of the two groups increased with decreasing temperature. Based on the results of the above two actual aquaculture experiments, the obtained algae beads have stable removal of 4+) and nitrite ions (10). The ability 'and algae beads can also inhibit the formation of ammonium (NV) and nitrite ions (N (V), the highest concentration is lower than the algae-free group, this result is also in the two groups of ammonium _4 + ) and nitrite ion (10) 2) removal efficiency and removal rate analysis, and the algae group also has a larger average removal per unit time; but when the culture medium contains ammonium (10) 4 + ) and nitrite ions (ΝΟ 〆) When present, due to the ingestion of nutrients by algae beads, the effect of removing phthalate ions (N〇3-) is less favorable. The two groups have an inhibitory effect on the vinegar group, resulting in a cumulative increase in nitrate. The concentration of ion (10) was lower; when the stocking density was not high, the pH values of the two test groups were similar, and there was no sharp fluctuation and they were kept within the pH range suitable for aquaculture water; the dissolved oxygen amount (D0) was changed twice. The group is not large, and is inflated by the aerator: 26 200827307 The change of temperature changes p ^ 々片 k 9 and the culture medium shows a higher / oxygenated h shape when the temperature is lower, and the temperature of the culture solution is also Affected by climate change, and the effect of algae beads on the light, two test periods The temperature is higher than the culture tank. It is apparent to those skilled in the art that various modifications and variations can be made without departing from the scope and spirit of the invention. Although the present invention has been described in terms of specific details, it should be understood that the invention should not be In fact, the different amendments that are obvious to those skilled in the art in the implementation of the present invention are also covered by the following patent applications. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view of a preferred embodiment of the present invention. Fig. is a graph showing the change of ammonium (NH4+) concentration in the algae group. The graph is a graph of ammonium (NH4+) concentration change in the control (no algae) group. #四图· is a graph showing the change of nitrite ion (N02-) concentration in the algae group. The variation of nitrite ion (N02-) k degree of the group "5^, θ• is the control (no algae beads)Fig. 0 PI · Less ^圃· is the concentration of nitrate ion (no3_) in the algae group Change graph. Figure 7 is a graph showing the change of nitrate ion (N〇3-) in the control (no algae) group. 27 200827307

[Explanation of main component symbols] (10) Culture tank (20) Aeration wastewater treatment tank (2 1 ) Algae bead tank (2 2 ) Buffer tank (3 0 ) Pumping motor (4 0 ) Lighting device 28 200827307 References Wu Jin, Liu Hongyu, Wu Shu, you translated, (all letters are also). 1992. Environmental microorganisms. Pp.50_ 54, PP.64·65. Science and Technology Books Corporation. Taipei. Thai shrimp farming pool follows 2. Ping, ί文, Weng Yilian, Qiu Qiuxia, Zhang Wenshu. 1996. Research on the water. COA Fisheries Special Issue 58:87_10 (^Taipei. • Lu Weiming, Lu Chongxing, Li Limei, Chen Bozhong. 1993. Study on the treatment of nitrogenous wastewater by immobilized blue-green algae. Proceedings of the 18th Wastewater Treatment Technology Symposium. Taichung.

4. Su Huimei. 1999. _The cultivation and utilization of biological materials. Ρρ·22-64. Taiwan Provincial Fisheries Laboratory. Keelung. 5. Chevalier P. and J. de la Noiie. 1985. Efficiency of immobilized hyperconcentrated algae for ammonium and orthophosphate removal from wastewaters. Biotechnology letters 17(6): 395-400. 6_ Garbisu, C·, J. M. Gil, M_ L Bazin, DO Hall and J. L. Serra. 1991· Removal of nitrate from water by foam-immobilized Phormidium laminomm in batch and contiimous-flow bioreactors. Journal of Applied Phycology 3:221-234. 7· Mak, AL and MD Trevan. 1988. Urea as a nitrogen source for calcium-alginate immobilized Chlorella. Enzyme Microb. Technol. 10:207-213. 8. Oswald, WJ 1978. The engineering aspects of microalgae. In Handbook of microbiology, 2nd Edition, Volume Π, AI Laskin and HA Lecheoalier, Eds·, ρρ·521-523, pp_531, ρρ·539-541· CRC Press, the United States· 9. Stein, JR 1973. Handbook of Phycological Methods: Culture Methods and Growth Requirements, Pp.7-24. Cambridge University Press. Cambridge. 10. Tam, NFY and YS Wong. 2000. Effect of immobilized Microalgal bead concentrations on wastewater nutrient removal. Environmental Pollution 107:145-151. 29

Claims (1)

200827307 X. Patent application scope: 1 · A method for treating aquaculture wastewater, comprising: providing a culture tank, an aeration wastewater treatment tank connected to the culture tank, a connection between the culture tank and the aeration wastewater treatment a pumping motor of the tank and a lighting disposed around the aerated wastewater treatment tank, placing the immobilized algae in the aerated wastewater treatment tank; and opening the pumping motor to make the tank The culture solution can be discharged to the aeration wastewater treatment tank for removal of nitrogen compounds, and then returned to the culture tank. 2. If you apply for a patent scope! The method for treating aquaculture wastewater according to the item, wherein the algae is a green alga. 3 · For the treatment of aquaculture wastewater as described in item 2 (4) of the patent, the green algae is chlorella. 4 The method for treating aquaculture wastewater according to claim 3, wherein the chlorella concentration is 1〇〇><1〇4 to 4〇〇〇χ1〇4 ceUs/mi. 5. In the case of f treatment of aquaculture wastewater as described in item 4 of the patent scope, the algae concentration is 1200 x 104 cells/ml. 6 The method for treating aquaculture wastewater as described in claim 1 of the patent application scope, wherein the illumination is continuously irradiated in the aerator type wastewater treatment tank. 7 · The method for treating aquaculture wastewater as described in item 6 of the patent scope is provided. The month and month are installed on both sides of the aerator-type wastewater treatment tank. 8 · As stated in item 7 of the application scope The treatment method of aquaculture wastewater, the light intensity of the ..., month device is 1, 〇〇〇 to 15 5, 0001 ux. 30 200827307 9 · If the application of the patent scope method, the treatment of the aquaculture wastewater described in the item C1 The light intensity of the device is 7,7〇〇1 rainbow. Method 'H Shen: The treatment method of the aquaculture wastewater described in the first paragraph of the patent range' The hydraulic retention of the algae groove is determined as κ6Λ raullcResidence : The application of the aquaculture wastewater mentioned in item 10 of the patent scope τ. ^The hydraulic retention time of the bead tank (Hydraulic Residence Time ’ HRT) is set to 2 hours. A slimming tank π; a treatment device for breeding aquaculture wastewater, comprising: a system for providing biological growth and having a water outlet and water inlet. The aeration-type wastewater treatment tank connected to the culture tank is provided with a plurality of algae beads, and is formed with a water inlet and a water outlet respectively connected to the water outlet of the culture tank and the water inlet, and the aerated wastewater treatment Two adjacent tank chambers are formed in the tank, one of which provides an algae bead and a buffer tank for the algae placement, and the water inlet is disposed on the tank wall of the algae bead, and the water outlet is located in the buffer tank a tank wall, wherein the culture liquid can pass through the algae bead tank sequentially to the buffer tank; a pumping motor connected to the aquaculture tank and the aerated wastewater treatment tank is a culture liquid in the culture tank After being extracted, the device enters the aeration wastewater treatment tank; and a lighting device disposed around the aeration wastewater treatment tank is irradiated on the aeration wastewater treatment tank. 1 3 · If the aquaculture wastewater mentioned in the scope of patent application No. 12 is used, the planting device, the opening of the algae channel of the aeration wastewater treatment tank is further provided with a closed open barrier network, the barrier network is prevented. The debris falls into the algae channel and also prevents the algae from flowing out of the algae channel. 1 4 · If the aquaculture wastewater treatment device described in item i 3 of the patent application is applied, the barrier network laid on the algae groove is a nylon mesh. 1 5 · The treatment device for aquaculture wastewater as described in claim 14 of the patent application 'the aeration wastewater treatment tank is placed above the culture tank, so that the water level in the aeration wastewater treatment tank is high In the culture tank, the culture liquid in the buffer tank can be returned to the culture tank. 16. The aquaculture wastewater treatment device of claim 15 wherein the pumping motor is a submersible motor and is placed in the culture tank. Η—, schema: as the next page 32