TW200820998A - Bactericidal composition - Google Patents

Abstract

A bactericidal composition comprising (A) an active oxygen compound, (B) an inorganic acid, (C) and a nitrate salt (provided that silver nitrate is excluded).

Description

200820998 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to a composition for killing H agents and a sterilization method using the same. [Prior Art] At present, there are various known agents for sterilization or disinfection, in particular, the use of sodium hypochlorite as a chlorine-based bactericide or oxygen as a peroxycarbonate in the production of peroxidation gas in water. For example, 'sodium hypochlorite is corrosive to metals, mixed with acidic substances, and it is produced in the environment to produce carcinogenicity. ^To achieve a high degree of bactericidal effect, hydrogen peroxide must be high concentration = long w At present, in the hydrogen peroxide, an organic acid is used in combination with a peracid formed by the reaction of the two as a bactericidal component, thereby obtaining a high bactericidal power for the combined organic acid, in terms of stability and bactericidal effect. Acetic acid 'but leaving a pungent odor and drainage _ (biochemical oxygen demand) big problem. J called Guang A6-71269 revealed that 'can be heated by the 过. 1% or less peroxidic gas solution to 30~603⁄4 3, 〇 曰 、 , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , Blending in hydrogen peroxide The additive is used to kill the composition of the spore. A disinfectant containing silver, inorganic acid fixative and hydrogen peroxide is disclosed in A8-225418. Chengxin 3 uxp/invention specification (supplement) grasp 12/96135333 5 200820998 The present invention relates to a bactericidal composition containing (A) an active oxide [hereinafter, referred to as (A) component], (B) a mineral acid [hereinafter, referred to as component (B)] and (C) Nitrate [hereinafter referred to as component (C)], but does not contain silver nitrate. The present invention provides a bactericidal composition which is formulated with (A) active oxide, (B) inorganic acid and (C) nitrate Further, the present invention provides a bactericidal product containing the sterilizing agent composition as an agent, and sterilizing the bactericidal composition or the sterilizing agent product in contact with the object. The method or the use of the above-mentioned composition or the above-mentioned product. [Embodiment] In JP-A 6-71269, if the sterilization of spores requires higher sterilizing power, sufficient effect cannot be obtained. Jp-A11_ 7638〇 Containing It is too acidic, so it does not solve the problem of irritating odor or b〇d in the drainage. It is disclosed in US4557898 that it can be added to the genus by the heating to about 5 generations, but the rate of spores is fastened, but the degree of chlorine peroxide is retained. The problem is quite high. If the concentration of hydrogen peroxide is high, the economy is poor, and the cost of draining after sterilization is also consumed. The disinfectant disclosed in JP-A8-225418 contains nitrate, and the acidity of silver is poor. The invention can provide an excellent bactericidal effect on the spore-forming bacteria, for example, 'the bud-forming bacteria can be killed at a concentration of 1 〇 6 cfu/mL or more, and the lion's low bactericidal composition can be provided. It

The sterilization method of the composition. A 312xp/invention specification (supplement)/96-12/96135333 6 200820998 According to the present invention, a bactericidal composition having a high bactericidal effect against spores, for example, a bacterium having a concentration of 1 〇 6 cfu/mL or more, can be obtained. Further, the bactericidal composition of the present invention contains a lower concentration of hydrogen and further lowers the B0D component. Hydrogen peroxide, etc. It is stable in storage. The present invention provides a bactericidal composition which has no irritating odor, low B0D, and reduced concentration of active oxide. Also, the sex is excellent. According to the sterilization method using the fungicide composition of the present invention, the microorganism can be killed by various microorganisms or various objects other than the human body. For example, examples of the bacteria include Escherichia coli, Saline's bacterium (-la), Staphylococc: s aureus, and Pseudomonas aeruginosa; and fungi include black bacillus and rosary Candida and the like. Further, it is a bacterial bud that exhibits strong resistance to the sterilizing agent and has heat resistance, such as Bacillus subtilis, or the like. Among them, the bacterial spore is a dormant cell produced under the environment of the proliferation of the genus, and has a multi-layered outer shell on the outer side of the bacterium. The resistance to chemicals or heat is high. Further, the bactericidal composition of the present invention can also have a high bactericidal effect on the spores. &lt;(A) Component> The active oxide of the present invention is preferably hydrogen peroxide or a compound which generates hydrogen in water. As the compound which generates the emulsified hydrogen in water, a solid compound containing a percarbonate or a persimmonate, particularly a sodium percarbonate, a granule or a powder, or a hydrogen peroxide aqueous solution may be used. The component (A) is effective for sterilization and protein removal. 312XP/Invention Manual (Supplement)/96-12/96135333 7 200820998 <Component (B)> Examples of the inorganic acid include hydrochloric acid, sulfuric acid, acid and can acid. Among them, hydrochloric acid, sulfuric acid, nitric acid, and phosphoric acid may be used singly or in plural. The component (B) is effective for sterilization and descaling. &lt;(C) component> Examples of the nitrate salt include an alkali metal salt of nitric acid such as sodium nitrate or potassium nitrate, and an alkaline earth metal salt of nitric acid such as magnesium nitrate or calcium nitrate, which can be used alone or in plural. Kind. Among them, preferred are sodium nitrate, potassium nitrate and magnesium nitrate. The component (C) is effective in sterilization and rust prevention. In particular, since the component (c) is further coexisted in the component (A) and the component (B), a synergistic effect can be obtained in the sterilizing action, and the total of the component (A), the component (B), and the component (c) can be reduced. The amount of use M is not used in the present invention. <Fungicide composition> The bactericidal composition of the present invention preferably contains 2 to 20% by weight as a high concentration bactericide composition. (Component, more preferably 4 to 1% by weight) Particularly preferably, it is 4 to 8% by weight. Further, it is preferably contained in an amount of 2 to 2% by weight, more preferably 4 to 15% by weight, particularly preferably 6 to 12% by weight. 'It is preferred to contain 1 to 20 parts by weight. / (C) component, = good 疋 1 1G weight I%, especially good 卜 5 weight%. Within these ranges, it is easy to handle and has excellent stability. The killing product of the present invention can be used to make (4) the high-concentration killing composition as described above, and (4) warming to the muscle and above, and c-contacting with the object to perform sterilization and cleaning. . Generally, the above-mentioned bismuth concentration biocide composition can be diluted to 10 to 150 times for use in 3UXP/invention specification (supplement)/96·12/96135333 200820998. When used, the diluent of the bactericidal composition of the present invention preferably contains 0.05 to 2.0% by weight (〇 component, more preferably 〇 = % by weight, particularly preferably 0.2 to 0.5% by weight. Preferably, it is a component containing 〇·5·lo% by weight, more preferably 01 to 8% by weight, particularly preferably 0.2 to 0.5% by weight. Further, it is preferable to contain (2 to 2 〇 /% by weight of the component (C), more preferably 0.05 to 1% by weight, particularly preferably 〇.1 to 〇.5% by weight. Within these ranges, a better killing effect can be obtained, the load on the drainage treatment is reduced, and the economy is also good. Further, if the component and the (Β) component are within these ranges, the influence on the device member is also smaller than ’. The composition containing the (Α) to (C) component at this concentration can be directly supplied to the bacteria and washed. The killing S #丨 composition of the present invention can be used for food plus 1 machine (4) for machine use. Examples of the medical device include an instrument for endoscopes such as an artificial dialysis device (including peripheral devices), an endoscope, and a biopsy forceps (biQptQme), etc., scissors, tweezers, sweet seeds, and needles. , colposcope, retractor, hose, test tube, catheter, hand push The fungicide composition of the present invention is particularly suitable for use in artificial dialysis devices. The bactericide composition of the present invention is preferably used for manual sterilization and cleaning. In the case of artificial a, six no 1 # 衣衣, artificial blood dialysis device as the object of the situation 'in addition to sterilization, it is required to remove the white, the, #, the main ice - mountain * teeth, decoction from the dialysate The calcium carbonate component, and then, the protein stain removed from the blood by dialysis. In the case of the endoscope, sterilization and removal are also required. Since it is white, it has become a suitable object for the composition of the fungicide of the present invention.勹令知月 杈 3 3 J 2XP / invention manual (supplement) / 96-] 2/96135333 200820998 δ has the role of inorganic acid, can be called (D) by the active oxygen contained By the action of the bactericidal composition of the present invention, carbonic acid #5 can be removed, and the protein can be removed and the organic acid further contained therein [the following protein is removed. As the component (D) of the present invention, r^^ 』Example No# nucleic acid, malic acid, tartar I, lactic acid, gluconic acid, glycolic acid, sputum H@X drunken thiol acetic acid, malonic acid , Hu Hushi, D mouth, sputum, pyruvic acid and bitter almond acid. In the present invention, the content of the '(D) component, 曲., 里里中中 辰 杀 kill ii agent site, preferably O.i'〗 詈0/, p red aa It is more difficult to get 曰ββ〇, 0.2~2% by weight, especially 疋0.4~1% by weight. In the special dry area, it is easy to handle, stable L, ° and 'excellent in protein removal, and considering the influence of (Α) component, especially 疋 on the stability of hydrogen peroxide, (d) component The content of the diluent in the diluent at the time of use is preferably from 〇2 to 2, more preferably from 0.03 to 〇15% by weight, particularly preferably from 〇5 to 〇ι% by weight. In the bactericidal composition of the present invention, the weight ratio of the component (4) to the (Β) component is preferably from 〇1 to 5, more preferably 0.5, in terms of bactericidal performance, in terms of (8) / (Α). 〜3, particularly preferably, the weight ratio of the component (1) to the component (c) is preferably (c)/(A), preferably 〇1 to 5, more preferably 〇2 to 2, especially The bactericidal composition of the present invention may contain the above-mentioned components and further contain other components such as the component (D) to form a liquid composition containing water, and other components other than the component (D). It can be mentioned that surfactants do not affect the stability of hydrogen peroxide (especially non-ion 312XP / invention manual (supplement) / 96-12/96135333 10 200820998, hydrogen peroxide, show tablets, amine III (t-T-phosphonic acid) and its salts, dipyridyl diphosphonic acid and its salts, ethylenediamine tetra (methylene) and salt , a method of sterilizing a substance, which is in contact with the object, root (8) into a top, ~ &quot;weight [(6) ingredient 〇.〇2~ water 杈The temperature of the microbial composition is 35. 〇 or more, and

Sterilization method in contact with the object. The contact of the bactericidal composition of the present invention with the Z-image object can be carried out by bringing it into contact with a suitable diluted liquid phase, and it is important to sufficiently contact the diluent with the object, in addition to the dipping method, in the pipeline cleaning, etc. In the case, a method of adjusting the flow rate at the time of passing the liquid or a method of adjusting the residence time after the liquid may be used. In the case where the object to be cleaned is a closed system including an infusion line or the like, it is preferred to carry out a cycle cleaning at an appropriate flow rate (e.g., Q1 to 50 liters/min). Also, the bubble method and the ultrasonic wave can be used in combination. [Examples] The following examples are set forth to illustrate the practice of the invention. The examples are set forth in the description of the invention and are not intended to limit the invention. (1) Bactericidal effect of bacterial spores Bacillus subtilis ATCC6051 was prepared in SCD Western culture medium (manufactured by Nippon Pharmaceutical Co., Ltd.) at 301: pre-culture for 4 weeks, and then collected in an appropriate amount on acacia medium. The colonies were suspended in sterile water of 312XP/invention specification (supplement)/96_12/96135333 11 200820998 1 mL, and microscopic examination was performed to confirm the formation of bacterial spores (hereinafter referred to as spores). After the suspension was centrifuged twice, the bacterial concentration was adjusted to 10 δ to 109 cfu/mL with an appropriate amount of sterile water. The spore suspension 〇"mL was inoculated into the sterilizing solution at the site of 19, and was prepared under the conditions of the killing of Table 1. Immediately thereafter, 0.1 mL of the sterilizing solution was added to the sc· medium containing i% by weight of sodium thiosulfate.

(In the M mL made by Sakamoto Pharmaceutical Co., Ltd., the sterilizing solution is not activated. The non-activated liquid 〇· 2 mL is applied to the acre, and the number of residual bacteria is determined by the standard acacia medium in t. The results are shown in the remaining part of the surface bacteria liquid is water at 35C to raise 24 hours 蚪 'counter formed in the Pei this test 宕焱 in Renzi cylinder. Received 丄田-... in each number' Again, Table 1 kill

312XP/Invention Manual (supplement)/96-12/96135333 12 200820998 〔1啭〕 is is 耸 涸蕹 涸蕹 衮 衮 哒 3 哒 3 3 3 3 3 SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS —4 co t—H CO τ—Η 蠖Η y &lt; Se ji4-^ « Pick 1 Pay Φ Side φ\ 激 Μ 5 5 WS r*H τ—Η s τ-Η r—HI ST—H ι —Η s τ-Η τ-Ηΐ ι—Η ◦ r—Η! P Ρ Ρ PP Ρ Ρ Ρ Ρ Ρ P Guide § sm III § s III 1 II ηοη IH od cvi 1 1 1 1 1 1 m *inTS Beam i Disc m Chlorinated sodium sulphate Naxo squid dish _ 1 | 1 1 II CO t-t CO τ-Η CO r-Η ηοη 1 1 I &lt;=&gt; CD CD cz&gt; 〇 · 〇· m mT\ • Ifrnl Ψ 碟 石 肖 酸钠 酸钠 v v vullf 1% If I 1 1 (Nl (XI 茭οα C&lt;I 笑·英· § Aftut Μ〇Π 1 I CD c· &lt ;=&gt; CD &lt;=5 C5 〇· c=&gt; 〇s mmS J# td: M old Μ Af*tt\ disc • 4% s negative m 1 (N1 oa 艺·CXI 〇〇tlftnl ηβη l CD cz&gt ; cr&gt ; cz&gt;&lt;〇&lt;=&gt;&lt;Z&gt;&lt;zSc=&gt; ^-H s MVS w minus § De Jj 1 Jj $ 1 Jj is5f Λ3 λ3 $ Jj Jj iiw Μ 1–4 ! Αα 1 τ-Η CO 1 ^H 1 LO 1 Τ—Η ζ〇1 ι—Η τ—Η 1 ι^Η (ΝΙ 1 ι—Η CO 1 Τ·Η 1 LO 1 rH CO 1 T—Η α eeKeI96 /3l-96/ff}Purple) Miscellaneous Distillation/dxzie 200820998 (2) The bactericidal effect of the sputum is Aspergilius niger IF〇6341, in the yam yam yam yam medium (Japan Pharmaceutical Co., Ltd.) Co., Ltd.), pre-cultured for 4 weeks at 25 。. The cells produced on the medium were collected and uniformly suspended in about 5 raL of sterile water using a glass homogenizer. After the suspension was washed twice with centrifugation, the bacterial concentration was adjusted to ι 7 to 108 cfu/mL with an appropriate amount of sterile water.

The spore suspension 〇.lmL was inoculated into the sterilizing liquid 19 red shown in Table 2, and it was allowed to act under the sterilization conditions of Table 2. 1You should η ! τ, " s person immediately, immediately add 0.1 mL of the sterilizing liquid to imL containing 1% sodium thiosulfate scDMp manufactured by Pharma Co., Ltd.) to make &lt;便 囷 囷 不 不 。 。 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋 蒋The number of viable cells was determined by this. The result is shown in D &lt; The remaining part of the sterilizing solution of the soil 2 is water. ^ Again, the table

312XP/Invention Manual (supplement)/96·12/96135333 200820998

Number of viable cells (cfu/mL) CD in Η CO τ-Η CSI τ-Η CO T—Η co_^ rH less than 50 less than 50 less than 50 less than 50 less than 50 less than 50 less than 50 sterilization conditions S? 120 minutes 120 minutes 120 minutes 120 minutes 1 120 minutes I 120 minutes | 90 minutes | 90 minutes 90 minutes 30 minutes 10 minutes 20 minutes 120 minutes P guide inch P inch Ρ PPP Guide PP § P § Ρ P Other ingredients of bactericide Weight 〇/〇1 1 1 § oi g τ-Η 1 1 1 1 1 1 1 m ^κτν 41 媸 氯化 氯化 氯化 氯化 氯化 氯化 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 1 (=&gt; CO TH; &lt;=&gt; CO τ-Η: c=&gt; CD 1—Η ◦· c=&gt; S cz5 CD VGQC Disc 41 Sample dish 41 41 Sodium nitrate Sodium nitrate Magnesium nitrate Sodium sulphate sodium nitrate sodium nitrate (B) component% by weight 1 1 cS CO CO cz&gt; CD οα οα CD CO CO Q· CN1 CZ5 CO CO CD S 〇· CO LO CD (NJ CO CD vrac Ufiul | barium sulfate acid sulfuric acid sulfuric acid sulfuric acid sulfuric acid (A) component% by weight 1 CD CNI 〇· οα CD CNI c6 CD &lt;&gt;3 «=5 03 cz&gt;C&lt;1&lt;z&gt; inch CNI &lt;Z&gt; r—Η 〇〇r—H ◦· 碳 Carbon hydroperoxide hydrogen peroxide Nitrogen peroxide Hydrogen peroxide hydrogen peroxide Hydrogen peroxide Hydrogen peroxide Hydrogen peroxide Hydrogen peroxide Carbonium 酉 Sodium percarbonate Hydrogen peroxide 1-1 1 CNI &lt;&gt;α 1 C&lt;I CO 1 CO 1 CNI LO 1 03 CO 1 (N1 1 &lt;NI CNI 1 (NI 1 1 &lt; NI LO 1 οα CD 1 CO !&gt;;- 1 C&lt;I ςι eeesI96/n-96/ipffii)»K^^麟μχπ£ 200820998 (3) Carbonate and protein soil removal The pipeline t-part is replaced by the contaminated stone under the following conditions: The hood hose is assembled into a ring in a recyclable manner via a pump, so that the sterilizing liquid of the watch is at a predetermined temperature (four). At the time of the scheduled cycle (4), the sister's contaminated rubber hose was removed and the internal pollution degree was evaluated. Sterilization: The cycle time is 40 minutes, the cycle temperature is 4〇t: and the cycle flow is 500 mL/min. The results are shown in Table 3. Further, the remainder of the sterilization 2 of Table 3 is water. * Contamination conditions of the rubber hose = about 40 cm of the inner diameter of the rubber hose, filled with standard dialysate [Fusang Pharmaceutical Industry Co., Ltd., Kindaly Liquid AF-j], and placed horizontally for 3 days. It was confirmed that the dialysate was removed after the precipitation of calcium carbonate. Further, about 2 mL of horse blood was added to the inner surface of the same silicone tube, and it was allowed to stand at a level of 6 &&gt;c for 2 hours to thermally degrade the horse blood and fix it. * Evaluation method of protein stains • After the cleaning, the silicone tube was removed, and about 5 cm of the blood-containing dirt was cut transversely, and the protein adhesion state was visually evaluated by indole black staining.评价 氺 炭 舞 之 之 附着 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 评价 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着 附着The Ca concentration of the trough was 'to obtain the amount of carbonation #5. It is converted into a hose of 1 m, expressed in units of CaCCh mg/m. 312XP/Invention Manual (Supplement)/9642/96135333 16 200820998 [(1)啭] 11 LO LO Less than 0· 05 Less than 0· 05 Less than 0· 05 Less than 0·05 - Less than 0.05 Protein Attachment _ VII Φ| dish sterilizing solution (D) component% by weight 1 1 1 S CD g 〇* CI&gt; τ-Η CD &lt;=&gt; r-(CD HSDC wire Afrui beam 1 m dish citric acid malic acid tartaric acid citric acid (C) component% by weight \ 1 c=&gt; CO y—i cz&gt; CO 〇· CD r 1 CD 〇· vane m&gt;T\ dish, sodium nitrate, sodium nitrate, sodium nitrate, nitrate surface (Β), component weight% 1 1 1 CNI οα &lt;=&gt; CD (NI ·· CO CO &lt;=&gt; § CD VQSC .Ifttil carbon m twitch η phosphoric acid (Α) component weight 〇/〇1 C3 CO CD cz&gt; inch (NJ 〇 ( (XI Q· endowment carbon hydrogen peroxide hydrogen peroxide hydrogen peroxide hydrogen peroxide percarbonization T—^ 1 CO οα 1 CO r—K 1 CO CN1 1 CO CO 1 CO ! CO LO 1 CO {^1 C Bi

Ll εΓη$εΙ96/3ι-96/(#:·)_^^κ ΜΧ 3ΐε 200820998 (4) Storage stability The bactericide stock solution of Table 4 was stored at 50 ° C for 14 days, and the active oxygen concentration was measured. The results are shown in Table 4. [Table 4]

Active oxygen retention rate after % of bactericide stock solution after 14 days (A) Component (B) Component (C) Component Other component type Weight % Type weight% Type weight% Type weight% Comparative Example 4-1 Hydrogen peroxide 5, 9 Sulfuric acid 9.0 None - Silver nitrate 3.25 72.9 Example 4-1 Hydrogen peroxide 5.9 Sulfuric acid 9.0 Sodium nitrate 3.25 None - 97. 5 312XP/Invention manual (supplement)/96-12/96135333 18

Claims (1)

200820998 X. Patent Application Scope 1· A bactericide composition' contains cerium active oxide ～...inorganic acid and (C) acid salt, but does not contain silver nitrate. 2. The bactericidal composition of claim i, which contains 〇· 〇5 to 2.0% by weight of 〇(active oxide, 〇〇5 to 1% by weight of (^ inorganic acid and 0.02 to 2, 0% by weight of bismuth acid salt. 3. The bactericidal composition of claim i or 2, which further contains 0.02 to 0.2% by weight of ()) organic acid. The bactericidal composition according to Item 1 or 2, wherein the active oxide is hydrogen peroxide. 5. The bactericidal composition of the patent scope 丨 or 2, wherein the non-stack S system is free of hydrochloric acid. And a bactericidal composition according to claim 1 or 2, wherein 2 i1 is selected from the group consisting of alkali metal nitrates and alkaline earths of nitric acid. One or more of the group consisting of metal salts. 2 The bactericidal composition of the scope of the patent or the second item is used for the scapular and cleaning artificial hemodialysis apparatus. , which is formulated with (A) active oxide, (B) hydrothermal acid and (C) nitrate to obtain 'but not There is silver nitrate. And:: heavy fungicide product 'which contains the bactericidal bismuth composition of claim 1 as a dose. The method of genus bacteria includes 1% 2 and 8 of the patent application scope. - The fungicide composition or the scope of patent application 帛 9 items of killing 312XP / invention manual (supplement) / 96-12/96135333 200820998 contact with the object of the fungus product. The method of killing bacteria, including making the application In the scope of patents 1, 2 and 8 ^, the killing of 8 hemp or the application of the goods (4) item 9 of the murder of the moon, the degree of the dish becomes 35 < lt; ^ above, 7 〇. Contact with the pair. The sub-claimer uses the fungicide for the product of the 9th product of the patent. The object of the patent is the scope of the patent scope of the second and eighth items of the fungicide penetration or the scope of the fungicide Disinfection and cleaning of artificial hemodialysis devices for products. 312XP/Invention Manual (supplement)/96-12/96135333 20 200820998 VII. Designation of representative drawings: (1) The representative representative of the case is: None (2) Simple description of the symbol of the representative figure: None 8. If there is a chemical formula in this case, please reveal the chemical formula that best shows the characteristics of the invention: 312XP/Invention Manual (supplement)/96-12/96135333 4