TW200812603A - Composition and use of skin care - Google Patents

Abstract

The present invention provides a composition and a use for skin care comprising a water insoluble fraction of water extract or alcohol extract of Astragalus membranaceus, or astragaloside compounds at an effective amount for improving skin texture, reducing wrinkles, UV protection, and anti-aging effect.

Description

200812603 IX. Description of the invention: [Technical field to which the invention belongs]

The present invention provides a skin care composition and uses thereof. In particular, A is a composition for improving skin texture, reducing wrinkles, preventing ultraviolet rays, and anti-aging, skin care, and uses thereof. [Prior Art] The present invention relates to a skin care composition and a material thereof, particularly a water-insoluble portion, an organic solvent portion or an active ingredient obtained by water extraction or alcohol extraction using a scutellaria y for use in a skin care composition and And its use. Also, Astragalus membranaceus comes from the plate jaundice of the plant known as Asiragah4s membnmaceus. Other aliases are: V 芪 (MilkVetchr〇〇t, which is a variety grown in the United States) and Huang-Qi. Astragalus is often used by traditional Chinese medicine practitioners to enhance or regulate the overall vitality of the body, promote digestion and nourish and strengthen the spleen. Many studies have also confirmed that jaundice contains therapeutically active ingredients, including polysaccharides that stimulate the immune system. One study showed that even if the subject of the terminal cancer had jaundice, the immune response could be increased two to three times. Another study showed that in animals treated with the immunosuppressant cyclophosphamide, jaundice still has the effect of a boost immune response. In China, cancer patients use jaundice anticancer drugs or radiation therapy to reinforce immunity because it helps protect cells in the body against heavy metals and chemical toxins. 200812603 Skin care and 'No research has indicated that Astragalus extract or Astragalus (IV) can be used for [Invention] Change: Provide a skin care composition and its use, which contains an effective amount for reducing wrinkles, UV rays and anti-aging ', 曰 (4) (4) like (4) Bu (10) this shrink) with water extraction = water insoluble parts. According to a specific example of the present invention, wherein the water-insoluble portion is obtained by a method comprising the steps of: extracting xanthine by a solvent of an alcohol or water to obtain an alcohol or water extraction*, and washing the alcohol with less water; Or water extract to obtain the water insoluble portion. In accordance with the present invention, a composition for skin care and use is provided: a cycloartanol compound of formula (1) # for anti-aging and anti-UV effective amount.

Formula (1) wherein R! is selected from the group consisting of hydrogen (H), hydroxy (〇ί1), 〇-acetamido ((9-acetyl), (9_木旅喃糖(〇_xyl〇) Pyranosyl), 〇-(2_ 醯木醯6 200812603 喃糖) (0(24〇61>only>4(^7 claws 11〇3}^1)), 0_(3_乙醯木旅口(0-(3-acetylxylopyranosyl)), (9·(2,3_diethyl eucalyptus) (O(2,3_diacetylxylopyranosyl)), <9·(2,4_二醯-xyl〇pyranosyl_(l-2)-pD-glucopyranosyl ), and (9-mu british sugar *-(1-2)-α-arabino-I-glycosyl (0-xylopyranosyl-(l-2)-cir-. arabinopyranosyl); Φ R2 is a group consisting of the following Selected from the group consisting of hydrazine, hydroxy, 0-ethyl fluorenyl, 〇-glucopyranosyl, and 0-methane saccharide; R3 is selected from the group consisting of hydrogen, hydroxy and hydrazine. - ethyl ketone; and

Other features and advantages of the invention will be set forth in the description which follows. The features and advantages of the present invention will be realized and attained by the <RTIgt; The above summary of the invention and the following embodiments are to be considered as illustrative and not restrictive. 7 200812603 [Embodiment] The present invention is described in detail below. In the present invention, the materials will be listed in detail in the examples. For the sake of the test: the terminology of the use of poverty will be described in detail. Secondly, the definition of this article is as follows: ==: The word "一" means - or more than - and also > Usage, only represents the singular meaning. As used herein, the term "effective amount" means a dose that provides an effect against ultraviolet light or a second aging in the general knowledge, "ht / % of material. For the disclosure according to the present invention: two rate 'Standard methods are easy to know. The knowledge and the heart of the heart-like test: the spoon month, {, a composition for skin care and its use' it contains the cover for the skin and the anti-aging right ▲ Anti-UV and anti-aging have a sputum of jaundice (Astraga!u or alcohol-derived water-insoluble part. According to the method of this method = step of the following = - solvent extraction of alcohol or water) Astragalus membranaceus, obtain an alcohol or water extract, and then use less water. The alcohol or water extract is insoluble in water. Called to the scorpion, according to a specific example of the present invention, wherein the water is insoluble in the scutellaria The compound of the formula 3 (1) of the cycloalkanol type (eycioartan 〇 1): 200812603

Formula (1) The genus is selected from the group consisting of hydrogen, hydroxy, 0-ethenyl, (9-woody glycosyl, 0-(2-ethyl-branched), 0-(3) -Euyue Mu Lun Glycosyl), 〇-(2,3-Bityl xylo-glycosyl), 0-(2/-diethyl-branched glutamic acid), 0-mu britose - (1_2X-D·匍 旅 旅 糖 、 及, and 0-木 喃 喃 - - (1·2) _ arabinopyranosyl; r2 is selected from the group consisting of 氲, hydroxy, α 醯Base, alpha grape ϋ 喊 糖 糖 、 、 、 、 、 、 、 、 、 r ; ; ; ; ; ; ; r r r r r r r r r r r r r r r r r r;

R4 is selected from the following group:

In a specific embodiment of the invention, wherein the water-insoluble portion of Astragalus contains a xanthine saponin compound of formula (II): 9 200812603

OH Formula (II) wherein RA, Rb, Rc and RD are from the group consisting of glucosinolate (Glc). , 乙基基,氢, In a preferred embodiment of the present invention, the yellow saponin compound of the formula (9) is not centered: the second package contains Rc as hydrogen and Rd is grape pentose Glc; for example, astragaloside/ is, according to another preferred embodiment of the present invention, I

Rc is 氲 and Rd is grape glucopyranosyl (Glc); for example, saponin = Kb 虱 according to a preferred embodiment of the present invention, Huang Lao 1 is 虱Rc is 虱, RD is hydrogen; for example, saponin HI . According to a preferred embodiment of the present invention, the water-insoluble portion of Astragalus contains the xanthine m compound of the formula (II), = not ~m 3 R /, ^Ra is 虱, Rb is hydrogen, Rc is hydrogen and rd For the grape vines (Glc); for example, astragalus buds zv. According to one of the inventions, the water-insoluble portion of the sputum of the formula (iJ) is packaged with RD, wherein Ra is a grape glucopyranosyl group (Glc) and Rb is hydrogen. D is a grape glucopyranosyl group (Glc); for example, saponin VI. In a preferred embodiment, the water-insoluble portion of Astragalus membranaceus contains a compound of the Japanese compound, wherein RA is an ethyl group and Rb is 氲, which is considered as 200812603.

Ethyl thiol and rd are grape glucopyranosyl (Gle). According to a preferred embodiment of the present invention, saponin IV is hydrolyzed by naringinase. As a result of the experiments in the present invention, the administration of the water-insoluble portion enhances the expression and secretion of collagen I and III in the cells, promotes the uptake of glucosamine and proline, and increases the hyaluronic acid (ha). The amount of performance that reduces the amount of matrix metalloproteinases (MMPs) and proteolytic activity. In the specific example of the present invention, the above-mentioned good effects can be obtained by treating the cells with the water-insoluble portion of the agent of about 25 to 40 pg/mL. In another aspect, the present invention provides a skin care composition comprising an anti-aging and anti-UV effective amount of a transdermal soap compound of the formula (I). The definition of the substituent is as described above. In a specific example of the present invention, the xanthine compound comprises the xanthine saponin compound of the formula (II), and the substituents and preferred embodiments are as described above. In a specific example of the present invention, the xanthine saponin compound comprises a cycloartanol compound of the formula (1), wherein 11 200812603

Ri is a base of &&; is (9-glucopyranosyl, R3 is a thiol, and 仏 is, for example, 哀 耆 耆 -6 -6-6 (9 D - ϋ ϋ 喃 喃 喃

φ Μ 蜀 / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / / It can enhance the performance of collagen I and collagen III in cells, promote the uptake of glucosamine and proline, increase the expression of hyaluronic acid, and reduce the expression and proteolytic activity of matrix metalloproteinase. It has an inhibitory effect on the amount of matrix metalloproteinase expressed by the excitation of ultraviolet radiation. White' avoids moxibustion and remedies the eyebrows and avoids the decomposition caused by matrix metalloproteinase. The scutellaria barbatum compounds of the formulas (1) and (II), and the water-insoluble portion of the extract of the scutellariae can be used as a medicament for preventing and treating skin (4) providing skin defense ultraviolet rays.

The insoluble part of the oyster extract can be borrowed from this 顼i ancient soil cr rb ^ 曰 The method known or commonly used by the skilled person to take the water insoluble part can be obtained by any standard method or cooked Method preparation. For example, astragalus extract is obtained by the following steps: providing jaundice, 200812603, especially in powder form, extracting with an alcohol to obtain an alcohol extract, and then extracting the alcohol extract with water to obtain a water-insoluble portion. In a preferred embodiment, the alcohol extract is the alcohol portion obtained by extraction with an alcohol such as 95% alcohol or methanol. However, it is to be understood that the alcohol extract is not limited to the insoluble portion of the xanthine obtained from the above method and the alcohol extracting portion. The present invention also provides a cosmetic cream comprising a water-insoluble portion of the xanthine extract, or a xanthine saponin compound according to the present invention. The φ cosmetic cream can be added to other ingredients used in the manufacture of basic creams. For example, such ingredients may include, but are not limited to, water, Triethylhexanoin isophthalic acid, behenyl alcohol, PEG-100 deuterated castor oil, glyceryl stearate SE, two Imethicone, Australian walnut oil, jojoba oil, hydrogenated printing ester, methyl paraben, DL_a-tocopheryl acetate, butyl hydroxybenzoate, phenoxyethanol and perfume. It should be noted that 'the invention is not limited to cosmetic applications, other musical applications, such as treating patients and lacking collagen eggs 'white I, collagen III, glucosamine, proline or hyaluronic acid, to 'The disease associated with enhanced expression or activation of the genus of the genus, is also within the scope of the application of the present invention. The invention is more specifically explained by the following examples. However, it should be understood that the invention should not be limited in any way by the examples. Example 1: Preparation of water-insoluble fraction of Astragalus membranaceus extract 13 200812603 1.0 kg of Astragalus root powder (from Formosa Kingstone Bioproduct International Co. Ltd.), extracted with 95% alcohol at 5 〇t for two hours, three times in total . The combined extracts were concentrated in vacuo to give about 127.8 g of an alcohol extract. The 127.8 g of the alcohol extract was washed three times with distilled water to obtain 33.9 g of a water-insoluble portion and 93.9 g of a water-soluble portion. 100.0 g of the alcohol extract was dissolved in distilled water and n-butanol (the ratio of the two solvents was 1:1) to obtain 63.0 g of a (n-)butanol soluble fraction. Example 2: Water-insoluble fraction of Astragalus membranaceus extract and the effect of Astragalus saponin on collagen expression in human HaCaT cells and HDF cells HaCaT cells were cultured in a spontaneously transformed human keratinocyte cell line HaCaT cells in humidified culture. In the box, at 37 ° C and 5% carbon dioxide and 95% air, 10% fetal calf serum (FBS), 100 IU / mL penicillin, 100 pg / rnL streptomycin, 2 mM sodium pyruvate and Culture in 1% non-essential amino acid (NEAA) in Dulbecco's Modified Eagle Medium (DMEM). The medium was changed every two days. After the cells are over, they are subcultured after trypsin treatment. For subculture, after transient treatment with 0.1% trypsin/ethylenediaminetetraacetic acid (EDTA) solution, cells were harvested and seeded at a 1:10 dilution ratio. The cells used in the study ranged between 12 and 45 generations. After inoculation and incubation of the cells for 24 hours, they were treated with the xanthine saponin compound for 24 hours. Control cultures were maintained in medium supplemented with vehicle (0.1% DMSO) 14 200812603. Under this culture condition, the effect of DMSO on growth and differentiation was not observed. HDF Cell Cultures Initial human skin fibroblasts (HDF) were purchased from Cascade Biologies (Portland, OR, USA) and were supplemented with 10% fetal calf serum, 100 IU/mL penicillin, 100 pg/mL streptomycin 106 Culture in medium. The cells used in the study ranged between 4 and 10 generations. The medium was changed every two days. After the cells are over, they are subcultured after trypsin treatment. For subculture, after transient treatment with 0.1% trypsin/EDTA solution, cells were harvested and seeded at a 1:10 dilution ratio. After lx105 cells were seeded in a 6-well culture dish and cultured for 24 hours, the xanthine saponin compound or a crude extract such as a butanol soluble portion and a water-insoluble portion of xanthine were treated for a specified period of time. Control cultures were maintained in medium supplemented with vehicle (0.1% DMSO). Under this culture condition, the effect of DMSO on growth and differentiation was not observed. The treated cells of the astragaloside compound are treated with a pure compound at a concentration of 0. 10 μM, such as xanthine saponin I (AS1), xanthine saponin II (AS2), xanthine saponin III (AS3), and astragaloside IV. (AS4), Astragalus saponin VI (AS6), Isoxanthin I (isoASl), Isoxanthin II (is〇AS2), and Astragalus saponin-6-(9-PD-glucosamine) (AA) Alternatively, the cells are treated with a crude extract of astragalus at a concentration of 0, 1, 10, and 40 pg/mL, such as a butanol soluble fraction and a water insoluble fraction of 15200812603. The cells are indicated by the indicated compound or crude. After the extract was treated for 24 hours, the cells were collected, and the collagen I and the sputum expressed in the whole cell extract or the collagen I and III secreted in the medium were analyzed and analyzed. Western blotting method was used for the culture medium and cell dissolution after the culture. The product was subjected to Western blot analysis. The cells were seeded at a density of IxlO6 cells/culture in a 6-centi dish 24 hours prior to treatment and 24 hours later with a specified concentration of the specified xanthine saponin compound for an additional 48 hours. The conditioned medium was centrifuged, concentrated twice and collected for analysis. Collagen and matrix metalloproteinase secreted into the medium. Wash the cells in 0.2 mL of lysis buffer at 4 ° C (containing 1% NP-40, 50 mM Tris-HCl (ρΗ7· 4), 180 mM sodium chloride, 1 mM EDTA, 1 mM PMSF, 1 mM sodium fluoride, 10 mM sodium citrate) was eluted for 30 minutes. After centrifugation at 17,500 g for 15 minutes, the cell lysate was collected. Supernatant. The protein concentration in the sample was measured using a Bicinchoninic acid (BCA) protein assay kit according to the manufacturer's method of use (Pierce, Rockford, IL, USA). An equivalent amount of 100 Pg) or cell lysate supernatant (50 μ§) was mixed with an appropriate volume of 4x SDS sampling buffer and separated by 8% SDS-PAGE gel. 8% SDS-PAGE gel The separated protein band was transferred onto a polyvinylidene fluoride (PVDF) membrane. The transferred PVDF membrane was freshly prepared with Tris_buffered 16 200812603 saline (TBS containing 0.1% Tween-20 and 7% skim milk powder). ) Wash twice and block at room temperature for two hours. Re-inject the PVDF membrane with anti-collagen eggs Any antibody of I and III or housekeeping protein α·tubulin (Santa Cniz Biotecnology, Santa Cruz, CA) was co-cultured for 18 hours at 4° C. Anti-goat antibody combined with horseradish peroxiase was used as the antibody. Secondary antibody. The signal was visualized by a reinforced chemiluminescence kit (Clontech, Palo Alto, CA, USA) and then exposed on an X-ray film. The following results are quantitative data from the Western blot analysis. In general, as shown in Table 1A, collagen I will be overexpressed in human HaCaT cells treated with different doses (0.01, 0.1 and ΐμΜ) of xanthine saponins such as AS 1, AS 2, isoAS 1, isoAS 2 and guanidine. which performed. Collagen I was also overexpressed in human HaCaT cells treated with 40 pg/mL of xanthine saponin extract.

Table 1A Collagen I compound in cells (μΜ) 0 0.01 0.1 1 AS1 1.00 1.13 0.72 1.22 AS2 1.00 0.86 L87 isoAS 1 1.00 1.13 1.19 isoAS2 1.00 1.52 1.41 ΑΑ 1.00 2.57 ~334^ 3.47 Extract (pg/mL) Collagen 丨I 0 1 10 40 Water-insoluble part 1.00 1.00 0.95 1.13 17 200812603 Human HaCaT treated with different doses (0·〇Μ)·1 and 1 μΜ of xanthine saponin compounds such as AS1, AS2, AS3 Collagen III in cells is overexpressed. Similarly, as shown in Table 1B, collagen III was also overexpressed in human HaCaT cells treated with different doses (1, 10 and 40 pg/mL) of crude extract. On the other hand, the amount of collagen-3 was secreted into the medium was measured to determine the effect of xanthan saponin on the release of collagen III by the cells. In Table 1B, when human HaCaT cells are treated with different doses (0.01, 0.1 and 1 μΜ) of xanthine saponin compounds such as AS4, AS6, isoAS1, collagen III is overexpressed in cell culture medium.

Table 1B Compound (μΜ) Collagen III 0 0.01 0.1 1 AS1 1.00 1.29 1.59 2.63 AS2 1.00 1.36 1.44 1.49 AS3 1.00 0.86 1.22 2.12 Extract (pg/mL) Collagen III in cells 0 1 10 40 Water insoluble Part 1.00 1.28 2.66 1.88 Compound (μΜ) Collagen III in the medium 0 0.01 0.1 1 AS4 1.00 0.79 1.13 1.16 AS6 1.00 4.55 4.27 3.86 18 200812603 isoASl 1.00 3.32 4.41 6.53 The collagen expression in human HDF cells was also determined. Based on the results listed in Table 2A, it was found that collagen 1 was overexpressed in human HDF cells treated with xanthine saponin compounds such as AS2, AS4, AS6, isoAS 1 and AA. At different doses (1, 10 and 40)

Kg/mL) of the crude extract of Astragalus, such as the soluble portion of the butanol of Astragalus and the water-insoluble portion, the collagen I in the treated HDF cells also overexpressed. In addition, the amount of collagen I present in the medium was also observed, which indicates that when the cells were treated with the xanthine saponin compound or the crude extract, an increased amount of collagen I was secreted into the medium.

Table 2A Collagen I in the medium of the collagen I compound (μΜ) ~Γ7^~~--- 0 0.01 0.1 1 0 0.01 0.1 1 AS2 1.00 1.02 0.73 1.26 1.00 1.24 1.15 1.02 AS4 1.00 1.16 1.33 1.07 1.00 1.24 1.11 L11 AS6 1.00 L18 1.41 1.30 1.00 1.19 1.29 1.25 isoASl 1.00 0.71 1.11 1.27 1.00 1.20 1.08 0.96 aa 1.00 1.04 1.85 1.17 1.00 0.97 0.95 1.52 Collagen I in the medium I extract (pg/mL) in the medium ---- -- 0 1 10 40 0 1 10 40 Butanol soluble fraction ---- 1.00 1.88 1.62 2.29 1·(10) 1.12 1.08 1.20 Water insoluble fraction - 1.00 0.95 0.99 1.63 1.00 1.28 1.40 1.72 19 200812603 In addition, as shown in Table 2B below As shown, when human HDF cells were treated with different doses of crude extract, the performance of collagen III was observed in both the cells and the medium. Therefore, the results of Western blot analysis showed that both collagen I and collagen III were overexpressed in human HaCaT cells and HDF cells, and the amount released into the medium was increased.

Table 2B Compound (μΜ) Collagen in cells 0 0.01 0.1 1 AS1 1.00 1.16 1.08 1.18 AS6 1.00 1.65 1.37 0.95 Extract (pg/mL) Collagen III in cells 0 1 10 40 Butanol soluble fraction 1.00 0.69 1.45 2.75 Water-insoluble part 1.00 2.71 3.10 1.81 Compound (μΜ) Collagen III in the medium 0 0.01 0.1 1 AS1 1.00 1.38 1.19 1.77 AS2 1.00 1.26 1.74 0.94 AS3 1.00 2.18 1.79 1.51 isoAS2 1.00 1.00 1.07 1.18 AA 1.00 1.03 1.62 1.34 Collagen ΠΙ in the medium 20 200812603 % Extract (pg/mL) Part οΓδο ι L55 10 40 232~~Ζ83 Example 3: Effect of saponins on proline uptake in human HaCaT cells Minute;}:

The proline uptake assay was performed as described in the literature (price: Ah / surgery: Μ 3 · 292, / surgery; as described). Briefly, HaCaT • cells were seeded at a density of 3x1 〇 4 cells/well in 24-well plates. The cells were cultured for 24 hours. The cells were treated for 48 hours in the absence (solvent control group) or in various concentrations of the xanthine saponin compound or the crude extract of astragalus. The treated cells were washed once with PBS, without The amino acid-containing, acid-containing medium (AAFM) was further cultured for 30 minutes. The treated cells were cultured with fresh AAFM containing 50 pg/mL ascorbate and a total concentration of 〇·5 mM and containing L-proline ( lpCi [3H] L-proline (American Radiolabelled Chemicals Inc, φ ARC, St. Louis, MO, USA). Wash with AAFM containing * unlabeled proline at specified intervals and at 200 pL 2% SDS ^ lysing cells. The cell lysate was centrifuged at 15,000 g for 15 minutes by counting 10 pL of cell lysate transferred to a UniFilter disk (Perkin-Elmer) with a filter at the bottom, ie It can detect the proline acid taken by the cells. The protein in the sample The concentration can be determined by the BCA protein assay kit described above. The proline acid accumulated in the cells is calculated and normalized to protein concentration, and the uptake rate is 21 200812603 per minute of cellular protein L·proline Molar number (nmol/min/mg) to indicate 〇

Table 3 Percentage of uptake rate (pmol / mg / min) (%) Control group 0.6904 ± 0.0225 100.00 0.1 μΜΑ81 0.8627 ±0.0741 124.97 0.1 μΜΑ82 0.9770 ±0.1119 141.52 0.1 μΜΑ83 0.7213 d= 0.0090 104.48 0.1 μΜΑ84 0.7628 ±0.1015 110.50 2_5 pg/mL 0.7196 ±0.0308 103.68 Water insoluble fraction G_25 pg/mL 0.7288 ±0.0401 105.56 Butanol soluble fraction 0.5G pg/mL 0.8230 ± 0.0573 119.20 Butanol soluble fraction 1 | ng/mL 0.7499 ± 0.0577 108.62 Butanol soluble In part, from the results shown in Table 3, we found that the crude extract increased the uptake of proline in human HaCaT cells by 105.56%, 119.2% and 108.62% compared with the control group, indicating that the crude extract had Promotes the effect of cellular uptake of proline. 22 200812603 Example 4: Effect of saponins on hyaluronic acid (HA) performance in human HaCaT cells Hyaluronic acid measurement Hyaluronic acid measurements were carried out according to the methods described in the literature (where Bi?C, 2(10) &lt; 3M-355). Briefly, HaCaT or HDF cells were seeded in 24-well plates and allowed to overfill. Immediately before the experiment, the cells were washed twice with serum-free medium to completely remove the hyaluronic acid accumulated during cell growth. The cells were then cultured for 48 hours in 0.5 mL of serum-free medium containing no or different compounds. After the indicated time, an aliquot of the medium was removed and centrifuged at 15,000 g for 5 minutes. The hyaluronic acid content in the supernatant was analyzed by an enzyme-linked immunosorbent assay (ELISA) kit (Echelon Bioscience, Salt Lake, UT). . The results listed are expressed as a multiple of the hyaluronic acid performance induced by xanthine in HaCaT cells. The concentration of the astragaloside used (if not otherwise indicated) is 0, 1, 10 μΜ for the pure compound of the astragaloside; 0, 0.5, 1, 2.5, 5 for the soluble portion of the butanol. 10, 40 pg/mL; 0, 1, 10, 40 pg/mL for the water insoluble fraction. The cells were treated with the indicated compounds for 24 hours, then the cells were harvested and the hyaluronic acid secreted in the medium was analyzed using the HA-ELIS A kit. Cells treated with 1 μΜ or 10 μΜ of xanthine saponin compounds AS1, AS2, AS4, AS6 showed an increase in the amount of hyaluronic acid secreted by the cells, as evidenced by an increase in the amount of hyaluronic acid in the medium. In human HaCaT cells, crude extracts at different doses (1, 10 and 40 pg/mL) also have the ability to promote the secretion of hyaluronic acid into the culture medium, as shown in Table 4A.

Table 4A Hyaluronic acid compound (μΜ) in the medium 0 1 10 AS1 1.00 1.45 1.36 AS2 1.00 1.98 1.31 AS4 1.00 2.27 1.31 AS6 1.00 1.13 1.38 Hyaluronic acid extract in culture medium (pg/mL) 0 0.5 1 2.5 5 10 40 Butanol Dissolved fraction 1.00 0.98 1.02 1.02 0.98 1.03 1.33 Extract (pg/mL) 0 1 10 L [Hydrolysis insoluble fraction 1.00 1.16 1.20 1.19 Quantitative analysis of hyaluronic acid synthetase 2 (HAS2) transcription by real-time quantitative reverse transcription polymerase chain The relative expression amount of HAS2 in HaCaT cells was determined by reaction (qRT-PCR). Total RNAs were isolated from cultured human cells using TRIzol reagent (Invitrogen, Irvine, CA, USA). 1 pg of RNA was reverse transcribed for 60 min at 37 ° C in 20 pL of transcription mix 24 200812603 containing 0.5 mM dNTP, 0.1 pg oligo-dT, 10 units of RNasin, l x PCR buffer ( 20 mM Tris-HCl with pH 8.3; 2.5 mM magnesium chloride; 50 mM potassium chloride) and 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen). The cDNA product was extracted with phenol, precipitated with alcohol, and resuspended in 20 μM TE (20 mM Tris-HCl and 1 mM EDTA, pH 8.0). qRT-PCR was performed using a Bio 7300 System (Applied Biosystem 7300 System) and a pre-developed Taqman Gene Expression Assays (Applied Biosystems, Foster City, CA, USA). The reaction mixture (total volume 20 μί) contains 2 pL of serially diluted cDNA - 10 μί Taqman Universal PCR Master Mix (Applied Biosystems) and 1 pL of human HAS-2 gene (assay number Hs00193435-ml, Applied Biosystems), or GAPDH primer mix (assay number Hs99999905 ml, Applied Biosystems). The amplification φ reaction was carried out under the following conditions: reaction at 50 ° C for 2 minutes, reaction at 95 ° C for 10 minutes, and then • 40 cycles of reaction at 95 t: 1 minute and 65 ° C for 1 minute. Two separate experiments were performed for each selected gene, and 3 replicates were replicated for each experiment. Relatively quantitative results were available using 7300 software (Applied Biosystems, Foster City, CA, USA).

It was calculated that the amount of HAS-2 expression in each sample was normalized to the amount of performance relative to GAPDH. As shown in Table 4B, in human HaCaT cells, whether the cells were treated with 0. 1 μΜ of xanthine compounds such as AS2, AS4, isoAS1 or is〇AS2, or 2.5 pg/mL, 5.0 pg/mL Astragalus soluble fraction of Astragalus membranaceus, or 25 200812603 treated with 40 pg/mL of water insoluble parts of Astragalus membranaceus, like 8_2 will overexpress. Therefore, the results of Tables 4A and 4B show that the xanthine saponin compound or the crude extract has an effect of promoting the performance of human CaCaT cells to break uric acid.

Table 4B HAS-2 compound in cells (μΜ) 0 0.1 AS2 AS4 isoASl isoAS2 .00 1.00Too' •00 2.73 1.41 1.12 1.28 Butanol soluble fraction

Extract (pg/mL) Extract (pg/mL) HAS-2 in water-insoluble cells 〇.9〇2.5 5.0 1.40 1.89 10 40 0.92 3.63 : Example 5: Astragalus saponin in human HaCa glycosamine uptake Affecting Glucosamine Uptake Analysis Glucosamine is the main smear of hyaluronic acid, w ^ team king, and it is early element. Therefore, the glucosamine intake by stem stalk can be presumed to be A * mouth &amp; u ^ . Hyaluronic acid binding in human ticks 26 200812603 In a glucosamine uptake test, HaCaT cells were seeded at a density of 3 x 104 cells/culture wells in 24-well plates and cultured for 24 hours. The cells were cultured for an additional 48 hours in the absence (solvent control) or in various concentrations of aglycone. The treated cells were washed twice with PBS and cultured in a medium free of glucose and amino acid (GSFM). After 2 hours, the cells were cultured in fresh GSFM containing 0.2 μίί [14C]-glucosamine (American Radiolabelled Chemicals Inc? ARC, St. Louis, MO, US A). The ® cells were washed twice with GSFM containing unlabeled glucosamine at specified intervals and plated at 200 μί of 2% SDS. The cell lysate was centrifuged at 15,000 g for 15 minutes. The glucosamine uptake by the cells can be measured by transferring the cell lysate of lOgL to a UniFilter disk (Perkin-Elmer) equipped with a filter at the bottom. The protein concentration in the sample can be determined using the BCA Protein Assay Kit described above. Calculate the glucosamine accumulated in the cell and normalize it to the protein concentration. The uptake rate is expressed as the count of protein per milligram per minute (cpm/min/pg). As shown in Table 5, when the cells are · 1 μΜ of xanthine saponin compound such as AS, AS2, AS3, isoAS1 and is〇AS2, the rate of glucosamine uptake increased. This shows that saponin has a promoting effect on glucosamine. _5_____ (cpm/gg protein) Control group 0.8242^00321 ~-:- 27 200812603 0.1 μΜΑ81 1.0482 ±0.0560 127.18 0.1 μΜΑ82 0.9561 ±0.0918 116.00 0.1 mMAS3 1.0129 Soil 0.0371 122.90 0.1 μΜΑ84 0.8931 ±0.0918 108.36 0.1 mMAS6 0.8471 ±0.0278 102.78 0.1 μΜΑΑ 0.9445 ± 0.0823 114.59 0.1 mMIsoASI 0.9589 d= 0.0665 116.34 0·1 pMIsoAS2 0.9226 Soil 0.0668 111.94 Example 6: Effect of xanthan saponin on matrix metalloproteinases (MMPs) in human HaCaT-l and HDF-1 cells Matrix metalloprotein Enzyme-l (MMP-l) is an enzyme that breaks down interstitial collagen and other extracellular matrix macromolecules. Matrix metalloproteinase-1 is the major collagenase. Therefore, the presence of matrix metalloproteinase-1 or its increased proteolytic activity indicates a decrease in the amount of collagen. Conversely, when the activity or performance of matrix metalloproteinase-1 is decreased, it means that the amount of collagen in the cells may be abundant. Ultraviolet Irradiation To understand the effect of xanthine saponin on the expression of MMPs in human cells, HDF or HaCaT cells were first treated for 24 hours with or without various concentrations of xanthan saponin. After the cells were washed with phosphate-buffered saline (PBS), HDF-1 and HaCaT were irradiated with UV-B (wavelength 312 nm) at 10-100 mJ/cm2 using an ultraviolet illuminator (UVItec unlimited, Cambridge, England). cell. The cells were collected and examined at 28 200812603. The cells were washed with serum-free medium and cultured for 24 hours. The effect of xanthine saponin on the performance of MMPs was analyzed by Western blotting method and the activity of MMPs was analyzed by zymography. All-trans-vitamin A acid (atRA) was used as a positive control group for inhibiting the activation and expression of MMPs caused by UV-B. Human HDF cells were treated with a water-insoluble portion of Astragalus membranaceus at the concentrations shown in the table for 24 hours, and then subjected to UV-B irradiation at 50 mJ/cm2. 24 • After hours, cells were collected for Western blot analysis. As shown in Figure 1, the expression of MMP-1 in cells not receiving UV irradiation was based on 値 (lane 1, control group), MMPq expression induced by UV irradiation in HDF cells (lane 2), all-trans RA (atRA) blocked MMIM performance induced by UV irradiation (lane 3, positive control group), and was induced by UV irradiation in cells pretreated with 0.1, 1, 1 〇, 40 μΜ of water-insoluble parts of Astragalus membranaceus The ΜΜΡ-1 performance was inhibited (lanes 4 to 7). The results showed that the water-insoluble portion of Astragalus membranaceus could effectively block the ΜΜΡ-1 expression caused by UV irradiation, indicating that the φ compound can protect collagen I. It is not to be decomposed by ΜΜΡ-1. Therefore, the water-insoluble part of Pu-Yu has potential as a drug for preventing and treating skin aging due to ultraviolet radiation. Proteolytic analysis of proteolytic activity of MMPs in HaCaT and HDF cells Basically, it was determined according to the method described in the reference (Cz&gt;c and called /999; 55:9(10)-9/7). The cells were treated with various concentrations of xanthan saponin for 48 hours 24 hours after inoculation. Centrifuge in conditioned medium, concentrate twice and store for inspection 29 200812603

Collagen and MMPs secreted into the culture medium. Place the cells in a dissolution buffer (containing 1% surfactant Triton, 50 mM pH 7.4)

The Tns-HC1 '180 mM sodium hydride, 1 mM EDTA) was dissolved and the protein concentration in the sample was determined using the above-described bisquinolinecarboxylic acid (BCA) protein assay kit. Mixing the sample of concentrated conditioned medium with the cell lysate and non-reducing electrophoresis loading buffer, and non-reducing

Electrophoresis was carried out in a state in which electrophoresis was carried out on 10% SDS-PAGE copolymerized with 2 mg/mL gelatin φ or road protein (Slgma, St. Louis, MO·USA). For zymography analysis of MMP-2 precursor and activated MMP-2, electrophoresis was carried out using a total of 20 pg of medium. After the electrophoresis was completed, the gel was renatured by soaking the gel twice with 25 g/L of Triton X-100 at room temperature for 10 minutes each. Then the gel is contained in 〇·2 Μ sodium chloride, 0.02%

Brij35 and 10 mM calcium chloride in 5 mM Tris-HCl (ρΗ7·5), at 37. (3 treatment for 18 hours. After the gelatinization was stained with Coomassie Brilliant Blue R-250 and deproteinized, the region of proteolytic activity was visualized in the gel after staining. With high sensitivity, the gel is further de-stained with 1% Triton X-100 « solution for 1 to 2 hours. This step increases the signal/noise ratio and allows for a weaker appearance at 92, 125 or even greater than 200 kDa. Gelatinase band. The zymogram was read using a computerized laser densitometer (Molecular Dynamics, Sunnyvale, CA, USA) with a knee imaging software (Image Quant software). Quantitative analysis of MMP-1 Korean recorded for immediate quantitative reverse transcription Polymerase chain reaction (qRT-PCR) was used to determine the relative expression of MMP-1 in HDF cells. The preparation method of total RNA and cDNA was as described in the previous section. qRT-PCR was applied to Bio 30 200812603 7300 system (Applied Biosystem 7300 System) and Pre-developed Taqman Gene Expression Assays (Applied Biosystems, Foster City, CA, USA). In addition to using human MMP-1 (assay IDHS00233958_ml, Appl The ied Biosystems) and GAPDH (assay ID Hs99999905jnl, Applied Biosystems) mixed primers, the reaction was carried out as described in the previous paragraph. The results in Table 6A represent quantitative data of zymogram analysis of MMP-2 protein in HaCaT cells, and HDF cells. qRT-PCR results for MMP-1. The concentrations used (if not otherwise indicated) are 0, 0.01, 0.1, ΙμΜ for pure compounds and 0, 1, 10, 40 pg/ for crude extracts. The cells were treated with the indicated compounds for 24 hours 'then the cells were then taken to analyze the V1MP-2 precursor in the whole cell extract and activate MMP-2. Electrophoresis was performed using 50 pg of cell extract sample. Results according to Table 6A When the cells are treated with various doses (〇〇i, oj, ImM) of xanthine saponin compounds such as AS1, AS2, AS3 and AS6, the proteolytic activity of MMP-2 in the conditioned medium is reduced or reduced. Collagen is abundant in human HaCaT cells.

Table 6A ΜΜΡ_2 Precursor/ΜΜΡ-2 Compound (μΜ) in the medium 〇0.01 0.1 1 31 200812603 AS1 1.00 0.57 0.57 0.86 AS2 1.00 0.89 0.89 0.67 AS3 1.00 0.76 0.57 0.81 AS6 1.00 0.89 0.67 0.74 As shown in Table 6B, Ο.ΙμΜAstragalus saponin compound AS1, AS2, isoASl, isoAS2 and AA in HDF cells, MMP-1

The performance of Mrna declined. In addition, when the cells were treated with various extracts at various doses (1 and 10 pg/mL), the amount of MMP_1 mRNA expression was decreased. Therefore, the results of Tables 6A and 6B show that an effective amount of the xanthine saponin compound or the crude extract can reduce the expression of MMP-1 and inhibit the collagen decomposition caused by the enzyme.

* Table 6B MMP-1 mRNA Compound (μΜ) 0 0.1 AS1 1.00 0.75 AS2 1.00 0.25 isoASl 1.00 0.6 isoAS2 1.00 0.71 AA 1.00 0.76 MMP-1 mRNA Extract (pg/mL) 0 1.0 2.5 5.0 Butanol soluble fraction 1.0 0.64 0.77 0.67 Extract (pg/mL) 0 1 10 32 200812603 ----- -----J 1.00 0.44 0.34 Example 7 : f Make-up cream containing Astragalus A makeup cream A cosmetic cream It is prepared by formulating a basic cream ingredient and an effective amount of Astragalus Soap Extract Extract as an active ingredient of a makeup cream. The ingredients for formulating basic creams include, but are not limited to, various waters, isodecyl isononanoate, butanediol, triisooctanoic acid glyceride, catechin, PEG-100 hydrogenated castor oil, glyceryl stearate SE, two One-second oil, Australian walnut oil, jojoba oil, sputum egg structure, methyl p-hydroxybenzoate, DL-α-tocopheryl acetate, butyl p-hydroxybenzoate, phenoxyethanol and spices, such as Table 7 shows. Table 7 Ingredients containing water-insoluble part of the extract of Astragalus membranaceus (100 g) Basic cream (100 g) Water added to 100 00 g (or %) to 100.00 g isodecyl isononate-- --- 7.00 g 7.00 g 5.00 g 5.00 g diisooctanoic acid glyceride - 3.00 g 3.00 g behenyl alcohol - 3.00 g 3.00 g 33 200812603 PEG-100 deuterated castor oil 2.00 g 2.00 g stearic acid glycerin SE 1.00 g 1.00 g — A cut oil 1.00 g 1.00 g Australian walnut oil 0.50 g 0.50 g Jojoba 0.50 g 0.50 g Hydrogenated lecithin 0.20 g 0.20 g Hydrazine paraben 0.15 g 0.15 g DL-α· Tocopheryl acetate 0.10 g 0.10 g Butyl p-hydroxybenzoate 0.10 g 0.10 g Phenoxyethanol 0.10 g 0.10 g Perfume 0.07 g 0.07 g Water insoluble fraction 0.02 g Example 8: Clinical trial subjects tested fifteen Healthy volunteers (2 females and 5 males) aged 35 or older are included in this clinical trial with their consent. Subjects were asked not to use any other cosmetic products on their faces during the entire trial period. The skin physical properties of the subject (skin pH, skin moisture content, water loss through the epidermis, skin surface fat, skin tone and brightness, skin elasticity and skin roughness) were evaluated 30 minutes after cleaning the subject's face. To provide basic measurements. Next, take an appropriate amount (about peanut size) of the cosmetic cream containing the extract of the present invention 34 200812603 ^ Also applied to the right face, and apply the same amount of basic cream to the left face (, after the control 30 minutes, The subject's face was tested the same as short-term efficacy. All tests were performed at about 24 ° C to 28 ° C, room temperature and 5G% to 6 G%. The maternal test is performed on three test areas (forehead, eye corner and upper cheek) selected on either side of the subject's face. The test point is about 2 cm in diameter. The sensor of the sensing element is Skin_pHMete^ ph 905 (C〇urage + Khazaka electr〇nic). The flat design of the probe head allows direct measurement of contact with the skin. - Lu color and bright 廑 (red spot index 舆 black 氡 招 招^ The measurement is based on the absorption and reflection of light. The probe of Mexamete^ Μχ Μ (Courage + Khazaka electronic) emits three kinds of light with special wavelengths of light. The receiver can measure the light reflected by the skin, illuminate crying - with the receiver Position ensures only diffusion and scattering It is measured: * When the emitted light 1 is fixed, the amount of light absorbed by the skin can be calculated. The melanin is measured by selecting a special wave corresponding to the different light absorption rate of the pigment. Selecting a special corresponding to the spectral absorption of hemoglobin Wave ^ Perform erythema measurement to avoid interference from other colored substances (such as bilirubin). The results of the two parameters are expressed as an index. Skin 袓E-rough _ plate 35 200812603 Skin roughness is measured by Skin Visiometer SV (Courage + Khazaka Electronic, Κϋΐη, Germany) measured, the skin inspection device is based on light transmission through a copy sheet containing a two-component fluorenone dyed in blue (the light absorption rate of which is known). The modified slide projector is directly absorbed by the light from the neon light and is absorbed by the thin layer. The amount of light absorption depends on the thickness of the fluorene ketone material. The video sensor is used as the electrical coupling device and the resolution is 64Ox 480 pixels black and white (b/w The CMOS-camera measures the amount of local light conduction and calculates the intensity of the light according to Lamber't and Beer's Law. The average skin measurements of the last 15 subjects aged 38 to 58 years are listed in Table 8. Table 8 Item pH erythema index Melanin index L value Surface volume left side -8.00 -4.25 -22.95 0.68 -2.79 3.29 Right side -11.19 0.22 -27.17 -1.18 -4.77 -7.77 According to the results in Table 8, the average pH of the subject's right skin decreased by 11.19%. Therefore, when the skin is treated with the cosmetic cream containing the scutellaria water of the present invention, it is expected that the skin will maintain a healthy state and the microbial growth will be minimized due to the slightly acidic environment. The erythema index was 0.22, and the subject's right side erythema was more pronounced than the left side, indicating that the right side of the face had better blood circulation. Black measured by the right face 36 200812603 The pigment index showed a 27.71% reduction in melanin. At the same time, the skin brightness expressed as the L value was evaluated together with the erythema index and the melanin index. The L value decreased by 1 · 18%, indicating that the skin looks brighter after one month of using the cosmetic cream. It can be seen that the subject's right face is whiter and radiant than the left face as a whole. The surface area of the wrinkles is measured to determine wrinkles in the skin of the subject's face. As shown in Table 8, the average surface area decreased by 4.77 〇/〇 after one month of using the cosmetic cream on the subject's right face, which showed a decrease in skin sensitivity. The volume (the skin volume relative to the skin surface area) decreased by 7.77%. This result shows that both the depth and the number of wrinkles are reduced. The compound may be desirable for skin care users at a frequency of several times per day or less&apos; such as once a day, once a week, once every two weeks, or once a month. The frequency of use is apparent to those skilled in the art and depends on a number of factors, such as, but not limited to, the age of the subject. Those skilled in the art will appreciate that the above specific examples can be modified without departing from the scope of the invention. Therefore, it is to be understood that the specifics of the invention are intended to cover the invention and the modifications of the scope of the invention. [Simple description of the schema] It is better to understand the contents of the invention and the reference to the drawings. In the figure: 37 200812603 Fig. 1 is a SDS-PAGE gel electrophoresis chart showing the inhibition of the amount of ΜΜΡ-1 expression in human HDF cells treated with xanthine saponin.

38

Claims (1)

200812603 Application for the patent garden 1. AStragalus membranaceus is a water-insoluble portion obtained by water extraction, which is used for the manufacture of skin care compositions, wherein the skin care composition is used for improving skin texture, reducing texture, Protects against UV rays and ageing.敝2· As requested by the requester, the water-insoluble portion of the Astragalus membranaceus is obtained by a method comprising the following steps: extracting scutellaria from a solvent such as ',,, ^ alcohol or water to obtain an alcohol or water The extract is washed with a small amount of water or water to obtain the water-insoluble portion. 3. The material of claim 1 wherein the water-insoluble portion of the scutellaria can be used locally for the desired skin area. • For the use of claim 1, wherein the water insoluble portion of the Astragalus membranaceus comprises a cycloartanol compound of the formula (I): ^3 Formula (I) The center is selected from the group consisting of hydrogen, hydroxyl, 〇_乙醯A chloropyranosyl, (9-(2-acetamidopentonyl), 〇-(3) _ 醯 哌 哌 ;; base) 0 · (2, 3- _ 醯 | 喃 喃 喃 、 、 、 39 39 39 39 39 39 39 39 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008旅 匍, 羟基, 旅 阿拉伯 喃 喃 ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( R R R R R R R R R R R R R R R R R R R R R R R R R R a base group, a quinone-glucopyranosyl group, and a 0-methane-glycosyl group; the R3 group is selected from the group consisting of hydrazine, a hydroxyl group, and a fluorenyl group; 5_ The use of claim 4, wherein the water-insoluble portion of the Astragalus membranaceus comprises the astragaloside saponin compound of formula (II): Formula (II) wherein RA, RB, RC and RD are selected from the group consisting of acetamyl, hydrogen and glucopyranosyl (Glc). 6. The use of claim 5, wherein Ra is an ethyl aryl group, Rb is an ethylenic group, Rc is hydrazine and RD is a glucomannan (Glc). 200812603 7·For the use of the claim $ &amp; , where Ra is an ethyl group, RB is hydrogen, 8 1 ^ helium and Rd is a grape heptanosyl group (Glc) 0 δ · as in (iv) the term R Ν Uses, wherein Ra is grape glucopyranosyl (Glc), Q ', , , gas 'RC is hydrogen and RD is hydrogen. 9. The use of the claim s + m , &gt; wherein RA is hydrogen, RB is hydrogen, Rc is 1L and R 2JL ^ D is glucopyranosyl (Glc). 1〇·If the request is ς), the use of Ra, is grape glucopyranosyl (Gle). B horse oxygen, RC is hydrogen and RD is grape glucopyranyl 11 · The use of the request s R 'where RA is ethyl hydrazine, rb is hydrogen, 12 士 di 乙 基 and ~ is glucomannan (Glc). A? If the demand item $ R ^^ is extended, where RA is hydrogen, RB is ethyl acetyl, 13 · such as V 2 and 4 ~ is grape glucopyranosyl (G1 C). A glutamyl group, R3Ar^ is a fluorenyl group 2 is 〇·_ I Κ3 is a thiol group and R4 is a oxime group. A scutellaria soap #人途, wherein (4) a line for the manufacture of a skin care composition; Heart = composition for anti-aging and defense against UV, chelates are compounds of formula (I): 200812603 Formula (1) wherein Ri is selected from the group consisting of hydrazine, hydroxy group, 0-ethyl aryl group, 0-wood britose group, 0-(2-ethyl-branched glutamyl group), Lu 0-(3) _B-brewed wooden brigade), 0-(2,3_2B-branched glutamic acid), Ο- (2,4 · Diethyl-branched glutamic acid), Ο _木旅喃糖-(l-2)-/3-D-匍 ϋ ϋ ϋ 糖 、 、 及 及 及 及 及 及 及 及 及 及 及 及 及 及 及 及 及 及 及 及 及 ( ( ( ( ( ( ( ( ( ( ( ( ( ( Selected from the group: hydrogen, hydroxyl, 0. acetyl, glucosinolate, and 0 _ 派 喊 ; ;; R3 is selected from the following group: hydrogen, hydroxyl and (9· 醯Base; Lu R4 is selected from the following group: 15. The use of claim 14, wherein the xanthine saponin compound comprises a compound of formula (II): 42 200812603 OH Formula (1) wherein R Ώ 醯 醯 Α, 二 ′ and R D are selected from the group consisting of 虱 and 匍 哌 哌 喃 喃 (glc). 16. According to claim 15 醯, R ί wherein Ra is ethyl acetyl, Rb is ethyl b 7 , ^ , C is hydrogen and RD is glucomannan (G1C). 17. If the claim 1 ς R, the inverse, where Ra is an acetamyl group, RB is hydrogen, c... oxygen and Rd is a grape-glycosyl group (Gic). 18 · If requested Use of minus 15, wherein Ra is a grape carragtose group C) 'RB is hydrogen' Rc is hydrogen and RD is hydrogen. 19 · For the purposes of cleaning jg, c, Bu, 15, wherein Ra is hydrogen, RB is hydrogen, Rc is gas and Rd is grape glucopyranosyl (Glc). 2 0 · If the request is 100] c 3 eight items 15 use, where (Glc) 'RB is hydrogen, rc is hydrogen and (Gle) 〇Ra is grape ΰ 喃 喃 喃 R 为 为 为 为 为 为 21 21 21 21 The use of the item 15, wherein Ra is an ethyl group, RB is hydrogen, Rc is an ethyl group and rd is a grape glucopyranosyl group (Glc). 22_ The use of claim 15, wherein Ra is hydrogen, Rb is ethyl acetyl, 43 200812603 is gas and Rd is grape glucopyranosyl (Glc). The use of the item 15, wherein Ri is a hydroxyl group, R2 is a 〇-glucosinose group, and I is a hydroxyl group and a hydrazine is 4. The use of claim 14, wherein the xanthine saponin compound is administered orally. 25 The use of claim 14, wherein the xanthine compound is used for the skin area in need thereof. The use of claim 15, wherein the xanthine saponin compound is administered orally. 7. The use of claim 15 wherein the xanthate soap compound 2 is used in a skin area in need thereof. 28. A skin care composition comprising an effective amount of jaundice (=stragaius membranaceus) for improving skin texture, reducing 29, 'texture, preventing ultraviolet rays and resisting aging, water extracting or alcohol extracting water insoluble portion Share. The composition of 28, wherein the water insoluble portion of the scutellaria, is prepared by the method comprising the following steps: extracting the scutellaria with an alcohol to obtain an alcohol extract; 1 extracting the alcohol extract with water to obtain Water insoluble part. The composition of claim 28, wherein the jaundice is topically applied to the area of the skin in need thereof. 〜 knife 200812603 31. The invention of claim 28, comprising the composition of formula (I), wherein the water of the scutellaria is insoluble, the cycloalkanol type compound: A Formula (I) Group B is selected from the group consisting of: hydrogen, county, ο二◦木 ° 喃 糖 、, 醯 哌 哌 哌 ) 、 、 -- -- -- -- -- -- -- -- -- -- -- 、 、 、 、 、 、 、 、 、 2,3_二乙醯ί丨二乙酿...glycosyl...pipetonose, 〇-paffyose-(ι_2)·α-arabinopyranose; system; Selected: hydrogen, secret, 〇-醢 醢 〇 葡萄 哌 哌 哌 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄 葡萄Selected from the following group The water-insoluble portion is the composition of claim 31, and the genus includes the scutellaria w compound of the formula (8): ... 45 32· 200812603 λ Formula (u) The rr, RC and RD in the back are selected from the group consisting of: ^ ^ Oxygen and glucopyranosyl (Glc) 〇 33. = Composition of the claim 32, wherein Ra is an ethyl group, Rb soil 'Rc Is hydrogen and Rd is a glucosyl group (Glc) 〇 34. If a composition of jg hv ' t , 32 is requested, wherein Ra is an ethyl group, RB is a rat hydrogen and RD is a grape glysyl group (Glc) ). 3 5 · If request jg. Groups 1 and 2, wherein ra is a grape-based glycosyl group (Glc), Rb is hydrogen, Rc is deuterium and RD is hydrogen. 36. The composition of claim 32, wherein Ra is hydrogen, RB is hydrazine, Rc is hydrogen and Rd is glucoheptanosyl (1) (4). The composition of the member 32, wherein ra is a grape sulphate (), Rb is hydrogen, Rc is hydrogen, and Rd is a composition of glucosinolates (Glc) 〇38. Ra is an ethyl group, RB is a nitrogen 'Rc is an ethyl group and rd is a grape glucopyranosyl group (Gic). 39. The composition of claim 32, wherein Ra is hydrogen, Rb is ethyl hydrazide, Rc is hydrogen, and rd is glucomannan (Glc). 46. The composition of claim 31, wherein I is a hydroxyl group, R2 is 0 glucopyranosyl (Glc), R3 is hydroxy and R4 is 41. A skin care composition comprising a xanthine saponin compound of formula (I) in an amount effective to resist aging and protection against ultraviolet light: Formula (I) wherein R! is selected from the group consisting of argon, hydroxy, hydrazine-ethyl ketone, (9 · xylenyl, (9-(2-ethyl-branched)), 0·(3 - B-brewed bromo-glycosyl), 0-(2,3-diethyl-branched glutamic acid), (9·(2,4·2B-brewed brigade) 0 - Mutan sucrose (1 - 2) stone D- 匍 派 派 喃 Ο Ο Ο 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 木 - - Selected from the group: hydrogen, hydroxy, oxime-ethenyl, glucosyl, and keto-glycosyl; R3 is selected from the group consisting of hydrazine, hydroxy and ethyl hydrazine; and 47 200812603 OH R4 is selected from the following group: 42. The composition of claim 41, wherein the xanthine saponin compound comprises a compound of formula (II): Formula (II) wherein RA, Rb, Rc and Rd are selected from the group consisting of ethyl acetate (Ac), argon (H) and glucomannan (Glc). 43. The composition of claim 42, wherein Ra is an ethylidene group, ~ is an ethyl aryl group, Rc is hydrogen, and RD is a glucosyl group (Glc). 44. The composition of claim 42, wherein RA is an ethylidene group, RB is nitrogen &apos; Rc is hydrogen and RD is glucomannan (G1C). The composition of claim 42, wherein Ra is a grape saccharide group (Glc), RB is hydrogen, Rc is hydrogen, and Rd is hydrogen. 46. The composition of claim 42 wherein RA is hydrogen and RB is hydrogen, 48 200812603 C is gas and Rd is grape glucopyranosyl (Glc). 4 7 Master Card • The composition of the fruit term 42 wherein Ra is a grape pentose base) Rb is hydrogen 'Rc is hydrogen and Rd is a grape-flavored sugar (Glc).田二 I 4 8 _如请| ^ Member 42 composition, where Ra is an ethyl group, RB is a gas acetyl group and RD is a glucosyl group (Glc). 49如士主杰, ‘ &gt; member 42 composition’ where ra is hydrogen and RB is 50 is hydrogen and Rd is a grape saccharide group (Gic) 〇. The composition of claim 41, wherein R1 is a hydroxyl group, R2 is Q_#^νΛΓ0Η glucopyranosyl group, R3 is a hydroxyl group and R4 is +0. The composition of claim 41, wherein the xanthine saponin compound is administered orally. The composition of claim 41, wherein the xanthine saponin 53 = is used in a skin area in need thereof. The composition of claim 42, wherein the xanthine saponin compound is administered orally. The composition of claim 42, wherein the xanthine saponin compound is topically applied to a skin area in need thereof. ^ 49