200805025 : 九、發明說明： 【發明所屬之技術領域】 本發明是有關於一種流體操控方法及系統、及其流體 裝置和致動裝置，特別是關於一種可適用於生化反應實驗 的流體操控方法及系統、及其流體裝置和致動裝置。 【先前技#f】 傳統的生化反應實驗是屬於勞力密集的工作，科學家 • 在實驗室裡面用試管、滴定管進行試劑生化反應，其主要 、 的工作是控制試劑的傳送、試劑與檢體的混合，並且等待 ， 足夠時間進行反應或者分離萃取。較具規模的實驗室已使 用機械手臂來進行這些反應的工作，節省了許多人力，並 且較精準的控制反應量，但是機械手臂的成本高，攜帶不 便，使用上有其偈限性。1989年德國的Manz教授提出 微全程分析系統（micro total analysis system，μ-TAS)概 念，其目的是將實驗室複雜的分析流程整合在數公分大小 ⑩的晶片上，顧名思義是在微小晶片上面就可以完成傳統實 驗室所做的分析流程’所以又稱為晶片上的貫驗室 (lab-on-a-cliip)，簡稱實驗室晶片，一般又稱為微流體 (micro-fluidic)晶片，其應用範圍相當廣泛，如醫學檢測、 新藥開發與食品檢測等等。 微流體元件及系統之主要發展為控制、感測、反應及 分析微量流體。關鍵元件包括微閥、微幫浦、微流量計、 微喷嘴、微流道、微混合器等，並可整合為不同功能之智 0957-A21667TWF(N2)；P64950004TW；tungming 5 200805025 慧化微型流體系統晶片。然而，從貯存區驅動少量（甚至微 里）>爪脰到此合區、反應區、檢測區，最後至廢液區，有許 夕不同的方法’包括注射或蠕動幫浦（Syringe and peristahic pumps)、電化學氣泡驅動（eiectr〇chemicai bubble generation)、還有聲波（3^011也〇3)、磁力（]11381161^3)、直流 或交流電動流（dc and ac electro-kinetics)與離心（centrifuge) 等。其中四種較重要且大有可為的方法包括離心、壓力、 聲波與電動流。 離心與壓力這兩種方法有較廣的流速範圍，而且在生 化分析領域中已有長久應用歷史，相關設備與零件成熟， 而電動流與表面積相關，適合管道進一步微小化的趨勢， 但其使用須要有電極與溶液接觸，其驅動力與液體的離子 強度與pH值相關，難以達到廣泛的應用，另外，聲波靠 固液界面振動產生動力，應用上較複雜，仍停在學術研究 階段。 習知以壓力驅動微流體的方法已經在美國專利公告第 6,793,753、6,899，137、6,929,030號中揭露，其利用氣壓推 動橡膠(PDMS)膜形變，作為控制閥件；又，在美國專利公 告第6,408,884、6,415,821號中，則揭露利用磁力驅動微 流體的裝置；又，在美國專利公告第6,627，159、6,814,953 號中，則揭露利用離心力驅動微流體的裝置。 另外，在美國專利公告第6,782,746、6,810,713、 6,802,342、6,953,058、6,203,296、6,644,944、6,561，224、 6,929,239、6,934,435、6,436,722 號、美國專利公開第 0957-A21667TWF(N2);P64950004TW；tungming 6 200805025 2003/0025129、200570084203號、以及中華民國專利公告 第590982、536524 ' 558549號中，則揭露其他微流體驅動 裝置和方法，其中包括幾篇以橡膠(rubber)類似的形變，產 生壓差的技術，將其摘要說明如下。 在美國專利公告第6,810,713號中，其利用轉子(rot〇r) 施加外力於彈性基板(elastic substrate)上，以周期性地擠壓 彈性基板上的微流道(microchannel)的既定部位，進而正確 地控制和傳遞微流道中的流體；在美國專利公開第 2003/0025129號中，.其將外力施加於彈性基板上，以逐漸 地沿著彈性基板上微流道的長度方向擠壓微流道，進而推 或拉微流道中的流體，以正石霍地傳遞微流道中的流體·，在 中華民國專利公告第590982號中，其，揭露一種微流體驅動 裝置’其架構係由雙層（上層和下層）基板、彈性中間層與 線性致動器所組成，其中線性致動器對彈性中間層往復施 加壓力，使彈性中間層和上層基板間的幫浦腔體產生收縮 以驅動流體。 【發明内容】 本發明係提供另一種以壓力傳送微流體的流體操控方 法及系統，其透過可形變材料之受壓變形，將流體元件内 微細管道成形為外伸的閥件與幫浦，並建構成流體裝置用 之簡易的水力卡匣，作為流體傳送的裝置。 根據本發明’提供一種流體操控系統，包括一致動裝 置以及一流體裝置，流體裝置設置於致動裝置中，且包括 複數俩可形變元件，可形變元件中之流體操控係藉由致動 0957-A21667TWF(N2)；P64950004TW；tungming 200805025 裝置之動作而進行。 又在本發明中，提供一種流體裝置，用以檢測一樣品， 包括一基底、一第一閥元件、一第一幫浦元件、一第二閥 元件、一第二幫浦元件、以及一第三閥元件，其中基底上 形成一第一流道、一第二流道、以及一第三流道，而第三 流道包括一檢測區，且和第一流道之間具有一重疊區，第 一閥元件與第一流道連通，用以控制樣品是否進入第一流 道中，第一幫浦元件與第一流道連通，用以將樣品吸入至 第一流道中，並通過重疊區，第二閥元件與第二流道連通， 用以控制一試劑是否進入第二流道中，第二幫浦元件與第 二流道連通，用以將試劑吸入至第二流道中，第三閥元件 與第三流道連通，用，以控制位於重疊區中的樣品是否藉由 試劑進入檢測區中。 又在本發明中，提供一種致動裝置，用以操控一流體 裝置，其中流體裝置包括複數個可形變元件，而致動裝置 包括一第一板元件、一第二板元件、複數個第一壓夾構件、 複數個第二壓夾構件、以及壓搾元件，其中第二板元件以 可轉動的方式與第一板元件連接，第一壓夾構件設置於第 一板元件上，弟二壓炎構件以分別和弟·^壓失構件中之任 一個對應的方式設置於第二板元件上，以和對應的第一壓 夾構件一起壓夾置於兩者之間的可形變元件，壓搾元件設 置於第二板元件上，.以壓搾可形變元件中的一個。, 又在本發明中，提供一種流體操控方法，包括下列步 驟:提供一流體裝置，其中流體裝置包括一第一流道、一 0957-A2166 丌 WF(N2);P64950004TW;tungming 200805025 第二流道、一第三流道、一第一槽、以及一第二槽，而第 三流道包括一檢測區，並和第一流道之間具有一重疊區， 第一槽與第一流道連通，第二槽與第二流道連通；供給一 樣品至第一槽中，供給一試劑至第二槽中；使試劑進入第 二流道中；使樣品進入第一流道中，且通過重疊區；以及 藉由試劑使位於重疊區中的樣品進入檢測區中，以進行檢 測。 又在本發明中，提供另一種流體操控方法，包括下列 步驟：提供一流體操控系統，其中流體操控系統包括一致 動裝置以及一流體裝置，致動裝置包括一第一壓搾元件和 一第二壓撺元件，流體裝置包括一第一流道、一第二流道、 一第三流道、一第一槽、以及一第二槽，而第三流道包括 一檢測區，並和第一流道之間具有一重疊區，第一槽與第 一流道連通，第二槽與第二流道連通；供給一樣品至第一 槽中，供給一試劑至第二槽中；使第二壓榨元件沿一第一 方向移動，使試劑進入第二流道中；在致動裝置中移動流 體裝置，並移動第一壓榨元件，以使樣品進入第一流道中， 且通過重疊區；以及在致動裝置中移動流體裝置，使第二 壓榨元件沿一第二方向移動，以使位於重疊區中的樣品藉 由試劑之推動而進入檢測區中，以進行檢測，其中第二方 向和第一方向相反。 藉由本發明之操控方法及系統，可以快速簡單地進行 生化反應貫驗。 為使本發明之上述及其他目的、特徵和優點能更明顯 0957-A21667TWF(N2)；P64950004TW；tungming 200805025 易懂，下文特舉一具體之較佳實施例，並配合所附圖式做 詳細說明。 【實施方式】 參考第1圖，本發明之流體操控系統1可用以在生化 反應實驗中檢測一樣品，且包括一致動裝置10以及一流體 裝置20，其中流體裝置20係以可移動的方式設置於致動 裝置10中。 _ 參考第2圖，流體裝置20包括一基底201、一第一閥 元件21、一第二閥元件22、一第三閥元件23、一第一幫 浦元件24、一第二幫浦元件25、一第一流道26、一第二 流道27、一第三流道28、——第一槽211、一第二槽221、 以及一第三槽231。 第一閥元件21、第二閥元件22、第三閥元件23、第 一幫浦元件24、第二幫浦元件25係為可形變元件，可由 橡膠等可形變的材料製成；又，參考第3a、3b圖，第一閥 ⑩ 元件21、第二閥元件22、第三閥元件23係可分別為一橡 膠管，其開關動作係藉由致動裝置10之第一和第二壓夾構 件14a、14b之壓夾動作來進行，其中第3a圖係表示閥元 件21、22、23為開啟狀態，而第3b圖係表示閥元件21、 22、23為關閉狀態；另外，第一閥元件21用以控制第一 槽211之樣品是否進入第一流道26中，第二閥元件22用 以控制第二槽221中之試劑是否進入第二流道27中，第三 閥元件23用以控制位於第三流道28之重疊區41中的樣品 是否藉由試劑之推力而進入第三流道28之檢測區42中， 0957-A21667TWF(N2)；P64950004TW;tungming 10 200805025 上述各閥元件之動作將在以下進一步說明。 另外，如第4a、4b圖所示般，第一幫浦元件24、第二 幫浦元件25係可分別為一橡膠管，其推拉動作係藉由致動 裝置10之第一和第二壓搾元件15、16之壓搾動作來進行， 其中第一幫浦元件24用以將樣品吸入至第一流道26中， 並通過重疊區41，而第二幫浦元件25用β將試劑吸入至 第二流道27中。 應注意的是第一幫浦元件24除了一端與第一流道21 連通之外，另一端係由一擋塊241阻塞，因此為一單向幫 浦元件；相對地，第二幫浦元件25除了一端與第二流道 22連通之外，另一端與一通氣口 251連通，因此為一雙向 幫浦元件。 又，應了解的是有關將上述可形變元件21、22、23、 24、25設置在基底201上的方式，係先在基底201欲放置 上述可形變元件的位置上預留穿孔，再將可形變元件放置 於基底之穿孔中，且使可形變元件之兩端分別與基底上之 流道連接，即可完成可形變元件之安裝；藉由穿孔之設置， 可方便致動裝置20從上下兩方向對設置於基底201上的可 形變元件進行壓夾和壓搾。 第一流道26連通第一閥元件21和第一幫浦元件24， 且係為如第2圖中的點線所包圍的流道；第二流道27連通 第二閥元体22.和第二幫浦元件25，且係為如第2圖中的 兩點破折線所包圍的流道；第三流道28連通第三閥元件 23、第一流道26、以及第二流道27，且係為如第2圖中的 0957-A21667TWF(N2)；P64950004TW；tungming 200805025 虛線所包圍的流道；又，第三流道28上包括一檢測區42， 且和第一流道26之間具有一重疊區41，應了解的是此重 疊區41也可作為针量用；另外，在檢測區42和重疊區41 之間係設有一混合區43，又’檢測區42中預先放置有欲 檢測的檢體’由於在混合區43和檢測區42中的反應過程 係為所屬技術領域中具有通常知識者所熟知，在此省略其 詳細說明。 第一槽211經由第一闊元件21與第一流道26連通， 用以儲存樣品；第二槽221經由第二閥元件22與第二流道 27連通，用以儲存試劑；第三槽231經由第三閥元件23 與第三流道28連通，用以收容來自第三流道28的樣品和 試劑；又，在本實施例中，樣品可為血液、血漿：、血清、 尿液、或其它體液，試劑可為清洗用的酸鹼缓衝液或含鹽 類溶液。 又，第一流道26、第二流道27、第三流道28、第一 槽211、第二槽221、以及第三槽231係一體成形於基底 201上，這些構造可利用塑膠射出成形技術、壓鑄模造法、 熱壓法、或是機械加工方式形成。 再次參考第1圖，致動裝置10用以操控流體裝置20， 包括一第一板元件11、一第二板元件12、一連接元件13、 複數個壓夾元件14、一第一壓搾元件15、以及一第二壓搾 元件Μ，其中第一板元件:11和_第二板元件12係分別輿連 接元件13樞接而可相對轉動；又，第二板元件12上係形 成有一第一溝槽12a、一第二溝槽12b、一第一孔洞12c、 0957-A21667TWF(N2)；P64950004TW；tungming 12 200805025 以及一第二孔洞12d，其中第一孔洞12c和第二孔洞12d 之位置係分別與流體裝置20之第一槽211和第二槽221 對應，以方便自外部供給樣品和試劑至第一槽211和第二 槽221申。 各壓夾元件14之位置係分別與流體裝置20上之閥元 件21、22、23對應，以控制閥元件21、22、23之開關； 又，壓夾元件14係分別包括一第一壓夾構件14a以及一第 二壓夾構件14b，其中第一壓夾構件14a係設置於第一板 元件11上，而第二壓夾構件14b以與第一壓夾構件14a對 應的方式設置於第二板元件12上，以和第一壓夾構件14a 一起壓夾置於兩者之間的閥元件。 應注意的是如第1圖所示般，各第一壓夾構件14a之 間係以非等間距的方式設置，亦即，各壓夾元件14之間係 以非等間距的方式設置；詳而言之，在第1圖中右侧的兩 個壓夾元件之間的間距小於左侧兩個壓夾元件之間的間 距，藉此可達成所希望的閥元件的開關動作，這將在以下 說明。 第一壓搾元件15之位置係與流體裝置20之第一幫浦 元件24對應，且以可移動的方式設置於第二板元件12之 第一溝槽12a中，以控制第一幫浦元件24之推拉。 第二壓搾元件16之位置係與流體裝置20之第二幫浦 元件25對應，_.以控制第二幫浦元件.25之推拉.，策二壓搾 元件16包括一移動件16a、一固定件16b、以及一彈性元 件16c，其中移動件16a以可移動的方式設置於第二溝槽 0957-A2166 丌 WF(N2); P64950004TW;tungming 13 200805025 12b中，固定件16b固設於第二板元件12上，彈性元件16c 連接移動件16a以及固定件16b，使移動件16a朝固定件 16b移動；又，彈性元件16c可為一拉伸彈簧。 另外，應了解的是第二壓搾元件16之構成並不限於上 述構成，其也可以電動方式驅動，例如，第二壓搾元件可 包括上述移動件16a和一馬達（例如許多商品化的步進馬達 [step moter]或伺月艮馬達[含直流或交流驅動]皆適用），藉由 馬達驅動移動件16a於第二溝槽12b中移動。 又，致動裝置10可更包括一第一突出部17以及一第 二突出部18，其係設置於第一板元件11上(參考第4a和 4b圖），且分別與第一壓榨元件15和第二壓榨元件16對 應，以配合第一壓榨元件15和第二壓榨元件16對流體裝 置20之幫浦元件24和25進行壓搾。 另外，為了方便流體裝置20在致動裝置10中定位和 移動，如第5圖之流體操控系統la所示般，致動裝置10 可更包括兩個第一定位部100，分別設置於致動裝置10之 第一板元件11和第二板元件12上（在第5圖中，僅顯示位 於第二板元件12上之第一定位部100)，其中第一定位部 100包括複數個定位孔101和一導槽102，而流體裝置20 可更包括與第一定位部100對應的兩個第二定位部200， 分別位於基底201之上面.和下面（在第5圖中，僅顯示位於 上面之第二定位部200)，且包括複數個定位銷210 ,藉由 定位銷210卡合於定位孔101中，使流體裝置20定位於致 動裝置中10之既定位置，且藉由定位銷210於導槽102 0957-A21667TWF(N2)；P64950004TW；tungming 14 200805025 中移動，可使流體裝置20在致動裝置10中沿一既定方向 移動；又，應注意的是第一定位部和第二定位部之設置數 目並不限於此，也可在流體裝置和致動裝置的底側和頂侧 均設置，而定位孔和定位鑕的設置數目也可視需要而改變。 本發明之流體操控系統之構成如上所述，以下參考第 6a〜8c圖說明本發明之流體操控方法。 欲在本發明之流體操控系統1上進行一生化反應實驗 時，首先，供給樣品至流體裝置20之第一槽211中，且供 給試劑至流體裝置20之第二槽221中；接著，如第6a圖 所示般，將流體裝置20移動至致動裝置10之壓夾元件14 壓夾第一閥元件21和第三閥元件23的位置，亦即，使第 二閥元件22開啟，而使第一閥元件21和第三閥元件23 關閉，與此同時，如第6b圖所示般，第二壓榨元件16之 移動件16a係使第二幫浦元件25沿第一方向A作動，以 對位於第二槽221中的試劑R產生一吸力，藉此使試劑R 自第二槽221進入第二流道27中，如第6〇圖所示般。 之後，如第7a圖所示般，將流體裝置20移動至致動 裝置10之壓夾元件14壓夾第二閥元件22和第三閥元件 23的位置，亦即，使第一閥元件21開啟，而使第二閥元 件22和第三閥元件23關閉，與此同時，如第7b圖所示般， 移動第一壓榨元件15使第一幫浦元件24作動，以對位於 第一# 221中的樣品S產生一吸力，藉此使樣品_S自第一 槽211進入第一流道26中，且通過重疊區41，如第7c圖 所示般。 0957-A21667TWF(N2)；P64950004TW；tungming 15 200805025 然後，如第8a圖所示般，將流體裝置20移動至致動 裝置10之壓夾元件14壓夾第一閥元件21和第二閥元件 22的位置，亦即，.使第三閥元件23開啟，而使第一閥元 件21和第二閥元件22關閉，與此同時，如第8b圖所示般， 第二壓榨元件16之移動件16a係使第二幫浦元件25沿第 二方向B作動，以對位於第二流道27中的試劑11產生一 推力，藉由試劑R之推動，位於重疊區41中的樣品S進 入檢測區42中，.如第8c圖所示般，應注意的是第二方向 B和第一方向A相反，且移動件16a在第二方向B上的移 動係可藉由彈.性元件之彈力來進行，至於移動件16a之移 動速度則可藉由變換不同的彈性元件來調整，進而調控第 二幫浦元件25中之流體速度。 在樣品S和試劑R進入測試區42之後，進入第三槽 231 中。 · 如上述，由於流體裝置中之可形變元件中之流體操控 係藉由致動裝置之動作而進行，可使生化反應實驗中，試 劑的傳送、試劑與檢體的混合更為簡單快速。 另外，為了使樣品之供給更為方便，如第9圖所示般， 流體操控系統lb可更包括一採樣器30，其與第一槽211 連通，藉此可將採檢的樣品直接供給至第一槽211中。. 雖然本發明已以較佳實施例揭露於上，然其並非用以 限定本發明，任何所屬技術領域中具有通常知識者，在不 脫離本發明之精神和範圍内，當可作些許之更動與潤飾， 因此本發明之保護範圍當視後付之申請專利範圍所界定者 0957-A21667TWF(N2)；P64950004TW；tungming 16 200805025 為準。200805025: IX. Description of the Invention: [Technical Field] The present invention relates to a fluid manipulation method and system, and a fluid device and an actuation device thereof, and more particularly to a fluid manipulation method applicable to a biochemical reaction experiment and System, its fluidic device and actuating device. [Previous technique #f] Traditional biochemical reaction experiments are labor-intensive tasks. Scientists • In the laboratory, test tubes and burettes are used for reagent biochemical reactions. The main work is to control the delivery of reagents, the mixing of reagents and specimens. And wait, enough time to react or separate the extraction. Larger laboratories have used robotic arms to perform these reactions, saving a lot of manpower and controlling the amount of reaction with precision, but the robotic arm is costly, inconvenient to carry, and has limited limitations in use. In 1989, Professor Manz of Germany proposed the concept of micro total analysis system (μ-TAS), which aims to integrate the complex analysis process of the laboratory on a wafer of several centimeters and size 10, as the name suggests is on a tiny wafer. The analytical process performed by traditional laboratories can be completed 'so called lab-on-a-cliip, referred to as laboratory wafers, also commonly referred to as micro-fluidic wafers. The range of applications is quite wide, such as medical testing, new drug development and food testing. The main developments in microfluidic components and systems are the control, sensing, reaction, and analysis of trace fluids. Key components include microvalves, micro-pulls, micro-flowmeters, micro-nozzles, micro-channels, micro-mixers, etc., and can be integrated into different functions. 0957-A21667TWF(N2); P64950004TW; tungming 5 200805025 Huihua microfluid System chip. However, driving a small amount (or even a small amount) from the storage area to the junction, reaction zone, detection zone, and finally to the waste zone, there are different ways of 'including injection or peristalic pumping (Syringe and peristahic) Pumps, electrochemical bubble drive (eiectr〇chemicai bubble generation), as well as sound waves (3^011 also 〇3), magnetic force (]11381161^3), DC or AC electrokinetics (dc and ac electro-kinetics) and centrifugation (centrifuge) and so on. Four of the more important and promising methods include centrifugation, pressure, acoustic and electrodynamic flow. The two methods of centrifugation and pressure have a wide range of flow rates, and have a long history of application in the field of biochemical analysis. The related equipment and parts are mature, and the electric flow is related to the surface area, which is suitable for the further miniaturization of the pipeline, but its use. It is necessary to have an electrode in contact with the solution. The driving force is related to the ionic strength and pH of the liquid, and it is difficult to achieve a wide range of applications. In addition, the sound wave is generated by the vibration of the solid-liquid interface, and the application is complicated, and it is still in the academic research stage. A method of pressure-driven microfluidics has been disclosed in U.S. Patent Nos. 6,793,753, 6,899, 137, 6, 929, 030, which are incorporated herein by reference to the entire disclosure of the disclosure of the utility of the utility of the utility of the utility of A device for driving a microfluid using a magnetic force is disclosed in U.S. Patent No. 6, 415, 821, the disclosure of which is incorporated herein by reference. In addition, U.S. Patent Nos. 6,782,746, 6,810,713, 6,802,342, 6,953,058, 6,203,296, 6,644,944, 6,561,224, 6,929,239, 6,934,435, 6,436,722, U.S. Patent Publication No. 0957-A21667TWF (N2); P64950004TW; tungming 6 200805025 2003/0025129, Other microfluidic driving devices and methods, including several techniques similar to rubber deformation, which generate a pressure difference, are disclosed in the specification of the Japanese Patent Publication No. 590,982, 536, 524, 558, 549, the disclosure of which is incorporated herein by reference. as follows. In U.S. Patent No. 6,810,713, an external force is applied to an elastic substrate by a rotor (rot〇r) to periodically press a predetermined portion of a microchannel on the elastic substrate, thereby correcting Controlling and transferring the fluid in the microchannel; in U.S. Patent Publication No. 2003/0025129, which applies an external force to the elastic substrate to gradually squeeze the microchannel along the length of the microchannel on the elastic substrate. In order to push or pull the fluid in the microchannel, to transfer the fluid in the microchannel in Orthodox, in the Republic of China Patent Publication No. 590982, which discloses a microfluidic drive device whose structure is composed of two layers (upper layer) And a lower substrate), an elastic intermediate layer and a linear actuator, wherein the linear actuator applies pressure to the elastic intermediate layer to retract the pump cavity between the elastic intermediate layer and the upper substrate to drive the fluid. SUMMARY OF THE INVENTION The present invention provides another fluid manipulation method and system for pressure-transmitting microfluids, which are formed by forming a micro-pipe in a fluid element into an overhanging valve member and a pump through compression deformation of the deformable material. A simple hydraulic cassette for a fluid device is constructed as a means of fluid transfer. According to the present invention, there is provided a fluid handling system comprising an actuating device and a fluid device, the fluid device being disposed in the actuating device and comprising a plurality of deformable elements, the fluid handling in the deformable element being actuated by 0957- A21667TWF (N2); P64950004TW; tungming 200805025 The operation of the device. Further in the present invention, there is provided a fluid device for detecting a sample, comprising a substrate, a first valve member, a first pumping component, a second valve component, a second pumping component, and a first a three-valve element, wherein a first flow channel, a second flow channel, and a third flow channel are formed on the substrate, and the third flow channel includes a detection region and an overlap region with the first flow channel, first The valve element is in communication with the first flow path for controlling whether the sample enters the first flow path, and the first pump element is in communication with the first flow path for drawing the sample into the first flow path and passing through the overlap region, the second valve element and the The second flow path is connected to control whether a reagent enters the second flow path, the second pump element is in communication with the second flow path for drawing the reagent into the second flow path, and the third valve element is connected to the third flow path Used to control whether the sample located in the overlap region enters the detection zone by the reagent. Still in the present invention, there is provided an actuating device for operating a fluid device, wherein the fluid device comprises a plurality of deformable members, and the actuating device comprises a first plate member, a second plate member, and a plurality of first a clamping member, a plurality of second clamping members, and a pressing member, wherein the second plate member is rotatably coupled to the first plate member, and the first pressing member is disposed on the first plate member The member is disposed on the second plate member in a manner corresponding to each of the respective pressing members to press and clamp the deformable member disposed therebetween with the corresponding first pressing member, the pressing member Provided on the second plate member to press one of the deformable members. Further, in the present invention, there is provided a fluid handling method comprising the steps of: providing a fluid device, wherein the fluid device comprises a first flow channel, a 0957-A2166 丌 WF (N2); a P64950004 TW; a tungming 200805025 second flow channel, a third flow path, a first groove, and a second groove, and the third flow path includes a detection area and an overlap area with the first flow path, the first groove is in communication with the first flow path, and the second The tank is in communication with the second flow channel; a sample is supplied to the first tank, a reagent is supplied to the second tank; the reagent is introduced into the second flow channel; the sample is introduced into the first flow channel, and passes through the overlap region; and the reagent The sample located in the overlap region is brought into the detection zone for detection. Still in the present invention, there is provided another fluid handling method comprising the steps of: providing a fluid handling system, wherein the fluid handling system comprises an actuating device and a fluid device, the actuating device comprising a first press element and a second pressure The 撺 element, the fluid device comprises a first flow channel, a second flow channel, a third flow channel, a first groove, and a second groove, and the third flow channel comprises a detection zone and the first flow channel Having an overlap region, the first slot is in communication with the first flow channel, the second slot is in communication with the second flow channel; a sample is supplied to the first tank, a reagent is supplied to the second tank; and the second press component is placed along the second Moving in a first direction to cause the reagent to enter the second flow channel; moving the fluid device in the actuation device and moving the first press member to cause the sample to enter the first flow channel and through the overlap region; and moving the fluid in the actuation device Actuating means for moving the second press member in a second direction such that the sample located in the overlap region enters the detection zone by the push of the reagent for detection, wherein the second direction and the first The opposite direction. With the control method and system of the present invention, the biochemical reaction can be quickly and easily performed. The above and other objects, features and advantages of the present invention will become more apparent. 0957-A21667TWF(N2); P64950004TW; tungming 200805025 is easy to understand, and a specific preferred embodiment will be described below in detail with reference to the accompanying drawings. . [Embodiment] Referring to Figure 1, the fluid handling system 1 of the present invention can be used to detect a sample in a biochemical reaction experiment, and includes an actuator 10 and a fluid device 20, wherein the fluid device 20 is movably arranged In the actuation device 10. Referring to FIG. 2, the fluid device 20 includes a base 201, a first valve member 21, a second valve member 22, a third valve member 23, a first pump member 24, and a second pump member 25. a first flow path 26, a second flow path 27, a third flow path 28, a first groove 211, a second groove 221, and a third groove 231. The first valve element 21, the second valve element 22, the third valve element 23, the first pump element 24, and the second pump element 25 are deformable elements, and can be made of a deformable material such as rubber; 3a, 3b, the first valve 10 element 21, the second valve element 22, and the third valve element 23 can each be a rubber tube, and the switching action is performed by the first and second crimping members of the actuating device 10. The clamping action of the members 14a, 14b is performed, wherein the 3a diagram indicates that the valve elements 21, 22, 23 are in an open state, and the 3b diagram indicates that the valve elements 21, 22, 23 are in a closed state; The component 21 is used to control whether the sample of the first slot 211 enters the first flow path 26, and the second valve element 22 is used to control whether the reagent in the second slot 221 enters the second flow path 27, and the third valve element 23 is used. Controlling whether the sample located in the overlap region 41 of the third flow path 28 enters the detection region 42 of the third flow path 28 by the thrust of the reagent, 0957-A21667TWF(N2); P64950004TW; tungming 10 200805025 The action will be further explained below. In addition, as shown in FIGS. 4a and 4b, the first pumping member 24 and the second pumping member 25 are respectively a rubber tube, and the push-pull operation is performed by the first and second pressing of the actuating device 10. The pressing operation of the elements 15, 16 is performed, wherein the first pumping element 24 is used to draw the sample into the first flow path 26 and through the overlap zone 41, while the second pumping element 25 draws the reagent into the second by β. In the flow channel 27. It should be noted that the first pumping element 24 is blocked by a stop 241 except that one end is connected to the first flow path 21, and thus is a one-way pumping element; in contrast, the second pumping element 25 is One end is in communication with the second flow path 22, and the other end is in communication with a vent 251, and thus is a bidirectional pumping element. Moreover, it should be understood that the manner in which the above deformable elements 21, 22, 23, 24, 25 are disposed on the substrate 201 is such that a perforation is reserved at a position where the substrate 201 is to be placed with the deformable element. The deformation component is placed in the perforation of the substrate, and the two ends of the deformable component are respectively connected with the flow channel on the substrate, thereby completing the installation of the deformable component; by the arrangement of the perforation, the device 20 can be conveniently driven from above and below. The directionally presses and presses the deformable elements disposed on the substrate 201. The first flow path 26 communicates with the first valve element 21 and the first pump element 24, and is a flow path surrounded by a dotted line in FIG. 2; the second flow path 27 communicates with the second valve element body 22. a second pump element 25, which is a flow path surrounded by a two-point break line in FIG. 2; the third flow path 28 communicates with the third valve element 23, the first flow path 26, and the second flow path 27, and It is a flow path surrounded by a dotted line as 0957-A21667TWF(N2); P64950004TW; tungming 200805025 in FIG. 2; further, the third flow path 28 includes a detection area 42 and has an overlap with the first flow path 26. The area 41, it should be understood that the overlapping area 41 can also be used as a needle amount; in addition, a mixing area 43 is disposed between the detecting area 42 and the overlapping area 41, and the detecting area 42 is pre-placed with a test to be detected. Since the reaction process in the mixing zone 43 and the detection zone 42 is well known to those of ordinary skill in the art, a detailed description thereof will be omitted herein. The first groove 211 is in communication with the first flow path 26 via the first wide element 21 for storing the sample; the second groove 221 is in communication with the second flow path 27 via the second valve element 22 for storing the reagent; the third groove 231 is The third valve element 23 is in communication with the third flow path 28 for receiving the sample and reagent from the third flow path 28; further, in this embodiment, the sample may be blood, plasma: serum, urine, or other The body fluid and the reagent may be an acid-base buffer or a salt-containing solution for washing. Moreover, the first flow path 26, the second flow path 27, the third flow path 28, the first groove 211, the second groove 221, and the third groove 231 are integrally formed on the base 201, and these structures can utilize plastic injection molding technology. , die-casting, hot pressing, or machining. Referring again to FIG. 1, the actuator device 10 is used to manipulate the fluid device 20, including a first plate member 11, a second plate member 12, a connecting member 13, a plurality of pinch members 14, and a first press member 15. And a second press member Μ, wherein the first plate member: 11 and the second plate member 12 are pivotally connected to each other to be relatively rotatable; and the second plate member 12 is formed with a first groove a groove 12a, a second groove 12b, a first hole 12c, 0957-A21667TWF (N2); P64950004TW; tungming 12 200805025 and a second hole 12d, wherein the positions of the first hole 12c and the second hole 12d are respectively The first tank 211 and the second tank 221 of the fluid device 20 correspond to facilitate the supply of samples and reagents from the outside to the first tank 211 and the second tank 221. The position of each of the clamping members 14 corresponds to the valve members 21, 22, 23 on the fluid device 20, respectively, to control the switching of the valve members 21, 22, 23; further, the clamping members 14 each comprise a first clamping member a member 14a and a second clamping member 14b, wherein the first clamping member 14a is disposed on the first plate member 11, and the second clamping member 14b is disposed in the second corresponding to the first clamping member 14a. On the plate member 12, a valve member interposed therebetween is press-fitted with the first press-clamping member 14a. It should be noted that, as shown in FIG. 1, each of the first press-clamping members 14a is disposed in a non-equal spacing manner, that is, each of the press-clamping members 14 is disposed at an unequal spacing; In other words, the spacing between the two press-clamping elements on the right side in Figure 1 is smaller than the spacing between the two press-clamping elements on the left side, whereby the desired switching action of the valve element can be achieved, which will The following instructions. The position of the first press member 15 corresponds to the first pumping member 24 of the fluid device 20, and is movably disposed in the first groove 12a of the second plate member 12 to control the first pumping member 24. Push and pull. The position of the second press member 16 corresponds to the second pump member 25 of the fluid device 20, to control the push and pull of the second pump member 25. The press member 16 includes a moving member 16a and a fixing member. 16b, and a resilient member 16c, wherein the moving member 16a is movably disposed in the second groove 0957-A2166 丌 WF (N2); P64950004TW; tungming 13 200805025 12b, the fixing member 16b is fixed to the second plate member 12, the elastic member 16c connects the moving member 16a and the fixing member 16b to move the moving member 16a toward the fixing member 16b; in addition, the elastic member 16c can be a tension spring. In addition, it should be understood that the configuration of the second press member 16 is not limited to the above configuration, and it may be electrically driven. For example, the second press member may include the above-described moving member 16a and a motor (for example, many commercial stepping motors). [step moter] or servo motor (including DC or AC drive) is applied, and the motor drives the moving member 16a to move in the second groove 12b. Moreover, the actuating device 10 can further include a first protruding portion 17 and a second protruding portion 18 which are disposed on the first plate member 11 (refer to FIGS. 4a and 4b) and respectively associated with the first pressing member 15. Corresponding to the second press element 16, the first press element 15 and the second press element 16 are pressed against the pump elements 24 and 25 of the fluid device 20. In addition, in order to facilitate the positioning and movement of the fluid device 20 in the actuating device 10, as shown in the fluid handling system 1a of Figure 5, the actuating device 10 may further comprise two first positioning portions 100, respectively arranged to be actuated On the first plate member 11 and the second plate member 12 of the device 10 (in FIG. 5, only the first positioning portion 100 on the second plate member 12 is shown), wherein the first positioning portion 100 includes a plurality of positioning holes 101 and a guide groove 102, and the fluid device 20 may further include two second positioning portions 200 corresponding to the first positioning portion 100, respectively located above and below the substrate 201 (in the fifth figure, only the display is located above The second positioning portion 200) includes a plurality of positioning pins 210. The positioning pin 210 is engaged in the positioning hole 101 to position the fluid device 20 at a predetermined position in the actuation device 10, and by the positioning pin 210. Moving in the guide groove 102 0957-A21667TWF (N2); P64950004TW; tungming 14 200805025, the fluid device 20 can be moved in the predetermined direction in the actuating device 10; further, it should be noted that the first positioning portion and the second positioning The number of settings is not limited to this, but also Body apparatus and a bottom side and a top side of the actuator means are provided, and the number of positioning holes and the positioning Zhi also changed as needed. The constitution of the fluid control system of the present invention is as described above, and the fluid control method of the present invention will be described below with reference to Figs. 6a to 8c. To perform a biochemical reaction experiment on the fluid handling system 1 of the present invention, first, the sample is supplied to the first tank 211 of the fluid device 20, and the reagent is supplied to the second tank 221 of the fluid device 20; As shown in Fig. 6a, the fluid device 20 is moved to a position where the clamping member 14 of the actuator device 10 clamps the first valve member 21 and the third valve member 23, that is, the second valve member 22 is opened, thereby enabling The first valve member 21 and the third valve member 23 are closed, and at the same time, as shown in Fig. 6b, the moving member 16a of the second press member 16 causes the second pump member 25 to act in the first direction A to A suction is generated for the reagent R located in the second tank 221, whereby the reagent R is introduced into the second flow path 27 from the second tank 221, as shown in Fig. 6. Thereafter, as shown in Fig. 7a, the fluid device 20 is moved to a position where the pinch member 14 of the actuating device 10 clamps the second valve member 22 and the third valve member 23, i.e., the first valve member 21 is caused. Opening, the second valve element 22 and the third valve element 23 are closed, and at the same time, as shown in Fig. 7b, the first press element 15 is moved to actuate the first pumping element 24 to be located at the first # The sample S in 221 produces a suction whereby the sample_S enters the first flow path 26 from the first groove 211 and passes through the overlap region 41 as shown in Fig. 7c. 0957-A21667TWF(N2); P64950004TW;tungming 15 200805025 Then, as shown in Fig. 8a, the clamping device 14 that moves the fluid device 20 to the actuating device 10 clamps the first valve element 21 and the second valve element 22 The position, that is, the third valve member 23 is opened, and the first valve member 21 and the second valve member 22 are closed, and at the same time, as shown in Fig. 8b, the moving member of the second press member 16 16a causes the second pumping element 25 to act in the second direction B to generate a thrust to the reagent 11 located in the second flow path 27, and the sample S located in the overlap region 41 enters the detection zone by the pushing of the reagent R 42. As shown in Fig. 8c, it should be noted that the second direction B is opposite to the first direction A, and the movement of the moving member 16a in the second direction B can be achieved by the elastic force of the elastic element. As a result, the moving speed of the moving member 16a can be adjusted by changing different elastic members, thereby adjusting the fluid velocity in the second pumping member 25. After the sample S and the reagent R enter the test zone 42, they enter the third tank 231. As described above, since the fluid manipulation in the deformable element in the fluid device is performed by the action of the actuating device, the transfer of the reagent, the mixing of the reagent and the sample in the biochemical reaction experiment can be made simpler and faster. In addition, in order to make the supply of the sample more convenient, as shown in Fig. 9, the fluid handling system 1b may further include a sampler 30 that communicates with the first tank 211, whereby the sample to be taken can be directly supplied to In the first slot 211. Although the present invention has been disclosed in its preferred embodiments, it is not intended to limit the scope of the present invention, and it is possible to make a few changes without departing from the spirit and scope of the invention. And the retouching, therefore, the scope of protection of the present invention is defined as 0957-A21667TWF (N2); P64950004TW; tungming 16 200805025 as defined in the scope of the patent application.
0957-A21667TWF(N2)；P64950004TW；tungming 17 200805025 【圖式簡單說明】 第1圖為本發明之流體操控系統之一實施例之示意 圖， 第2圖為第1圖中之流體裝置之示意圖； 第3a圖為第2圖中之閥元件之示意圖，其中閥元件係 為開啟狀態； 第3b圖為第2圖中之閥元件之示意圖，其中閥元件係 _為關閉狀態； 第4a圖為第2圖中之第一幫浦元件之示意圖； 第4b圖為第2圖中之第二幫浦元件之示意圖； 第5圖為本發明之流體操控系統之一變形例之示意 圖； 第6a〜8c圖係表示本發明之流體操控方法之示意圖； 以及 第9圖為本發明之流體操控系統之一變形例之示意 •圖。 【主要元件符號說明】 1、la、lb〜流體操控系統 10〜致動裝置 11〜第一板元件 12〜第二板元伴 12a〜第一溝槽 12b〜第二溝槽 0957-A21667TWF(N2)；P64950004TW；tungming 18 200805025 12c〜第一孔洞 12d〜第二孔洞 13〜連接元件 14〜壓夾元件 14a〜第一壓爽構件 14b〜第二壓失構件 15〜第一壓榨元件 16〜第二壓梓元件 16a〜移動件 16b〜固定件 16c〜彈性元件 17〜第一突出部 18〜第二突出部 20〜流體裝置 21〜第一閥元件 22〜第二閥元件 23〜第三閥元件 24〜第一幫浦元件 25〜第二幫浦元件 2 6〜第一流道 27〜第二流道 28〜_第三流_道........... 100第一定位部 101〜定位孔 0957-A2166 丌 WF(N2);P64950004TW;tungming 19 200805025 102〜導槽 200〜第二定位部 201〜基底 210〜定位銷 211〜第一槽 221〜第二槽 231〜第三槽 241〜擋塊 251〜通氣口 O957-A21667TWF(N2)；P64950004TW；tungming 200957-A21667TWF(N2); P64950004TW;tungming 17 200805025 [Simplified illustration of the drawings] Fig. 1 is a schematic view showing an embodiment of a fluid control system of the present invention, and Fig. 2 is a schematic view of the fluid device of Fig. 1; 3a is a schematic view of the valve element in FIG. 2, wherein the valve element is in an open state; FIG. 3b is a schematic view of the valve element in FIG. 2, wherein the valve element is in a closed state; FIG. 4a is a second Schematic diagram of the first pump component in the figure; Fig. 4b is a schematic diagram of the second pump component in Fig. 2; Fig. 5 is a schematic view showing a modification of the fluid control system of the present invention; Figs. 6a-8c BRIEF DESCRIPTION OF THE DRAWINGS Fig. 9 is a schematic view showing a modification of the fluid control system of the present invention; and Fig. 9 is a schematic view showing a modification of the fluid control system of the present invention. [Description of main components] 1. la, lb~ fluid handling system 10~actuating device 11~first plate element 12~second plate element 12a~first groove 12b~second groove 0957-A21667TWF(N2 P64950004TW;tungming 18 200805025 12c~first hole 12d~second hole 13~connecting element 14~clamping element 14a~first pressing member 14b~second pressure reducing member 15~first pressing element 16~second Compression element 16a to moving member 16b to fixing member 16c to elastic member 17 to first protruding portion 18 to second protruding portion 20 to fluid device 21 to first valve member 22 to second valve member 23 to third valve member 24 ~First pumping element 25~Second pumping element 2 6~First flow channel 27~Second flow channel 28~_third flow_dao...........100 first positioning part 101 〜Positioning hole 0957-A2166 丌WF(N2); P64950004TW; tungming 19 200805025 102~ guiding groove 200~second positioning portion 201~substrate 210~positioning pin 211~first slot 221~second slot 231~third slot 241 ~ Block 251 ~ vent O957-A21667TWF (N2); P64950004TW; tungming 20