TW200734461A - A method of using GS as a selectable marker in glutamine auxotrophic cells - Google Patents

A method of using GS as a selectable marker in glutamine auxotrophic cells

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Publication number
TW200734461A
TW200734461A TW096115526A TW96115526A TW200734461A TW 200734461 A TW200734461 A TW 200734461A TW 096115526 A TW096115526 A TW 096115526A TW 96115526 A TW96115526 A TW 96115526A TW 200734461 A TW200734461 A TW 200734461A
Authority
TW
Taiwan
Prior art keywords
glutamine
exogenous dna
selectable marker
protein
dna sequence
Prior art date
Application number
TW096115526A
Other languages
Chinese (zh)
Other versions
TWI352120B (en
Inventor
John Birch
Robert Charles Boraston
Martyn Shaw
Original Assignee
Lonza Biologics Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Biologics Plc filed Critical Lonza Biologics Plc
Publication of TW200734461A publication Critical patent/TW200734461A/en
Application granted granted Critical
Publication of TWI352120B publication Critical patent/TWI352120B/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A glutamine-auxotrophic human cell transfected with an exogenous DNA sequence encoding a protein or an exogenous DNA sequence capable of altering the expression of an endogenous gene encoding a protein and an exogenous DNA sequence encoding a glutamine synthetase, wherein these exogenous DNA sequences are located on one or more than one DNA construct, said transfected cell capable of producing said protein and capable of growing in a glutamine-free medium.
TW96115526A 2002-01-17 2003-01-15 A method of using gs as a selectable marker in glu TWI352120B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0200977A GB0200977D0 (en) 2002-01-17 2002-01-17 Glutamine-auxotrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium

Publications (2)

Publication Number Publication Date
TW200734461A true TW200734461A (en) 2007-09-16
TWI352120B TWI352120B (en) 2011-11-11

Family

ID=9929201

Family Applications (2)

Application Number Title Priority Date Filing Date
TW96115526A TWI352120B (en) 2002-01-17 2003-01-15 A method of using gs as a selectable marker in glu
TW092100765A TWI349038B (en) 2002-01-17 2003-01-15 Glutamine-auxothrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium

Family Applications After (1)

Application Number Title Priority Date Filing Date
TW092100765A TWI349038B (en) 2002-01-17 2003-01-15 Glutamine-auxothrophic human cells capable of producing proteins and capable of growing in a glutamine-free medium

Country Status (4)

Country Link
CN (2) CN100497634C (en)
GB (1) GB0200977D0 (en)
MY (2) MY137536A (en)
TW (2) TWI352120B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2839976C (en) * 2011-06-21 2020-07-21 University Of Guelph Screening for nitrogen fixation
MA44976A (en) * 2016-05-11 2019-03-20 Amgen Inc DIRECT SELECTION OF CELLS EXPRESSING HIGH LEVELS OF HETEROMERIC PROTEIN USING INTRAGENIC GLUTAMINE SYNTHETASE SUPPLEMENTATION VECTORS
WO2024156285A1 (en) * 2023-01-29 2024-08-02 Shanghai Zhenge Biotechnology Co., Ltd. Glutamine synthetase marker for recombinant expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8601597D0 (en) * 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
ES2281133T3 (en) * 1997-07-23 2007-09-16 Roche Diagnostics Gmbh IDENTIFICATION OF HUMAN CELLULAR LINES FOR THE PRODUCTION OF HUMAN PROTEINS THROUGH THE ENDOGENA ACTIVATION OF GENES.

Also Published As

Publication number Publication date
MY137536A (en) 2009-02-27
CN1966682A (en) 2007-05-23
GB0200977D0 (en) 2002-03-06
MY136860A (en) 2008-11-28
CN101492656B (en) 2011-05-04
TWI349038B (en) 2011-09-21
CN101492656A (en) 2009-07-29
CN100497634C (en) 2009-06-10
TW200307047A (en) 2003-12-01
TWI352120B (en) 2011-11-11

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MM4A Annulment or lapse of patent due to non-payment of fees