SI1585810T1 - Method for culturing cells in order to produce substances - Google Patents

Method for culturing cells in order to produce substances

Info

Publication number
SI1585810T1
SI1585810T1 SI200331791T SI200331791T SI1585810T1 SI 1585810 T1 SI1585810 T1 SI 1585810T1 SI 200331791 T SI200331791 T SI 200331791T SI 200331791 T SI200331791 T SI 200331791T SI 1585810 T1 SI1585810 T1 SI 1585810T1
Authority
SI
Slovenia
Prior art keywords
glucose
limitation
dgl
order
culturing cells
Prior art date
Application number
SI200331791T
Other languages
Slovenian (sl)
Inventor
Thomas Link
Ruth Essers
Kerstin Skopnik
Jochen Gatgens
Thomas Noll
Christian Wandrey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SI1585810T1 publication Critical patent/SI1585810T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars

Abstract

Culturing cells in order to produce substances, comprises setting a glucose limitation in the culture solution. Culturing cells in order to produce substances comprises setting a glucose limitation in the culture solution. The extent of glucose limitation (DGL) equals qGlc/Glc max, where qGlc is the current observed specific glucose consumption rate, and qGlc max is the maximum known glucose consumption rate for the cells in question. DGL lies between 0-1, where 0 is a complete limitation, and 1 represents no limitation (i.e. a complete glucose surplus). According to the invention the DGL is greater than or equal to the DGL that leads to the exclusive preservation of the cell and is 0.5.
SI200331791T 2002-11-27 2003-11-07 Method for culturing cells in order to produce substances SI1585810T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10255508A DE10255508A1 (en) 2002-11-27 2002-11-27 Process for cultivating cells for the production of substances
EP03767406A EP1585810B1 (en) 2002-11-27 2003-11-07 Method for culturing cells in order to produce substances
PCT/DE2003/003693 WO2004048556A1 (en) 2002-11-27 2003-11-07 Method for culturing cells in order to produce substances

Publications (1)

Publication Number Publication Date
SI1585810T1 true SI1585810T1 (en) 2010-06-30

Family

ID=32318751

Family Applications (3)

Application Number Title Priority Date Filing Date
SI200332602T SI2275530T1 (en) 2002-11-27 2003-11-07 Method for cultivating cells for the production of compounds
SI200331791T SI1585810T1 (en) 2002-11-27 2003-11-07 Method for culturing cells in order to produce substances
SI200332473A SI2226381T1 (en) 2002-11-27 2003-11-07 Method for cultivating cells for the production of compounds

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SI200332602T SI2275530T1 (en) 2002-11-27 2003-11-07 Method for cultivating cells for the production of compounds

Family Applications After (1)

Application Number Title Priority Date Filing Date
SI200332473A SI2226381T1 (en) 2002-11-27 2003-11-07 Method for cultivating cells for the production of compounds

Country Status (12)

Country Link
US (2) US20060127975A1 (en)
EP (3) EP2226381B1 (en)
JP (2) JP4469283B2 (en)
AT (1) ATE462013T1 (en)
CY (2) CY1110267T1 (en)
DE (2) DE10255508A1 (en)
DK (2) DK1585810T3 (en)
ES (3) ES2749217T3 (en)
HU (1) HUE028862T2 (en)
PT (1) PT1585810E (en)
SI (3) SI2275530T1 (en)
WO (1) WO2004048556A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2011514T3 (en) 1997-03-21 2012-03-26 Chugai Pharmaceutical Co Ltd Preventive or therapeutic agent for sensitized T cell mediated diseases comprising IL-6 antagonist as an active ingredient
UA80091C2 (en) 2001-04-02 2007-08-27 Chugai Pharmaceutical Co Ltd Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist
GB2401040A (en) 2003-04-28 2004-11-03 Chugai Pharmaceutical Co Ltd Method for treating interleukin-6 related diseases
DK1623019T4 (en) * 2003-05-15 2017-03-27 Wyeth Llc LIMITED GLUCOSE SUPPLY TO ANIMAL CELL CULTURE
PE20091174A1 (en) 2007-12-27 2009-08-03 Chugai Pharmaceutical Co Ltd LIQUID FORMULATION WITH HIGH CONCENTRATION OF ANTIBODY CONTENT
DE102008002210A1 (en) * 2008-06-04 2009-12-10 Evonik Degussa Gmbh Process for the fermentative production of erythropoietin
AU2015203309B2 (en) * 2009-10-26 2016-05-05 Chugai Seiyaku Kabushiki Kaisha Method for the production of a glycosylated immunoglobulin
AU2016210748B2 (en) * 2009-10-26 2018-03-01 Chugai Seiyaku Kabushiki Kaisha Method for the production of a glycosylated immunoglobulin
BR122022001178B1 (en) * 2009-10-26 2022-10-04 F. Hoffmann-La Roche Ag METHOD FOR THE PRODUCTION OF A GLYCOSYLED IMMUNOGLOBULIN AND ITS USE
TWI537386B (en) * 2009-12-22 2016-06-11 Ｄｓｍ智慧財產有限公司 The preparation of caprolactam from 6-amino caproic acid obtained in a fermentation process
US8580264B2 (en) 2010-11-08 2013-11-12 Genentech, Inc. Subcutaneously administered anti-IL-6 receptor antibody
JP6563910B2 (en) 2013-07-04 2019-08-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Interference suppression immunoassay for detecting anti-drug antibodies in serum samples
US10544395B2 (en) 2014-03-19 2020-01-28 Pfizer Inc. Method of cell culture
JP7001585B2 (en) 2015-09-23 2022-02-04 ファイザー・インク Cell and cell culture methods
WO2020115655A1 (en) 2018-12-06 2020-06-11 Pfizer Inc. Cells with reduced inhibitor production and methods of use thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4657863A (en) * 1982-07-02 1987-04-14 Celanese Corporation Stabilization of a mutant microorganism population
JPH066054B2 (en) * 1987-10-15 1994-01-26 帝人株式会社 Method for culturing animal cells
CA2078546A1 (en) * 1990-04-03 1991-10-04 Phillip W. Berman Methods and compositions for vaccination against hiv
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
US5252216A (en) * 1992-03-24 1993-10-12 Smithkline Beecham Corporation Protein purification
US5443968A (en) 1992-05-01 1995-08-22 Teijin Limited Fed batch culture method for protein secreting cells
GB9506249D0 (en) * 1995-03-27 1995-05-17 Karobio Ab Media for insect cell cultures
WO1998041611A1 (en) * 1997-03-20 1998-09-24 Regents Of The University Of Minnesota Process for the continuous culture of cells
US6338964B1 (en) * 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration

Also Published As

Publication number Publication date
JP2006507821A (en) 2006-03-09
SI2226381T1 (en) 2016-05-31
EP2275530A3 (en) 2012-05-23
ES2749217T3 (en) 2020-03-19
US20060127975A1 (en) 2006-06-15
CY1117558T1 (en) 2017-04-26
ATE462013T1 (en) 2010-04-15
EP1585810B1 (en) 2010-03-24
ES2340377T3 (en) 2010-06-02
JP4469283B2 (en) 2010-05-26
WO2004048556A1 (en) 2004-06-10
EP1585810A1 (en) 2005-10-19
JP5221573B2 (en) 2013-06-26
PT1585810E (en) 2010-05-05
EP2275530B1 (en) 2019-08-07
EP2226381A1 (en) 2010-09-08
DE10255508A1 (en) 2004-06-17
US20140329277A1 (en) 2014-11-06
DK2226381T3 (en) 2016-04-11
EP2275530A2 (en) 2011-01-19
DE50312554D1 (en) 2010-05-06
ES2567161T3 (en) 2016-04-20
CY1110267T1 (en) 2015-01-14
SI2275530T1 (en) 2019-11-29
JP2010110331A (en) 2010-05-20
EP2226381B1 (en) 2016-03-09
HUE028862T2 (en) 2017-01-30
DK1585810T3 (en) 2010-06-07

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