TW200724687A - Immobilized proteins based on oil body - Google Patents

Immobilized proteins based on oil body

Info

Publication number
TW200724687A
TW200724687A TW094145952A TW94145952A TW200724687A TW 200724687 A TW200724687 A TW 200724687A TW 094145952 A TW094145952 A TW 094145952A TW 94145952 A TW94145952 A TW 94145952A TW 200724687 A TW200724687 A TW 200724687A
Authority
TW
Taiwan
Prior art keywords
aobs
oil body
proteins based
immobilization
immobilized proteins
Prior art date
Application number
TW094145952A
Other languages
Chinese (zh)
Other versions
TWI361833B (en
Inventor
Chung-Jen Chiang
Hung-Chen Chen
Yun-Peng Chao
Tze-Cheng Tzen
Original Assignee
Nat Univ Chung Hsing
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Univ Chung Hsing filed Critical Nat Univ Chung Hsing
Priority to TW94145952A priority Critical patent/TWI361833B/en
Publication of TW200724687A publication Critical patent/TW200724687A/en
Application granted granted Critical
Publication of TWI361833B publication Critical patent/TWI361833B/en

Links

Abstract

The present invention is related to a new method based on artificial oil bodies (AOBs) was proposed to achieve protein refolding and immobilization in one step. Without adding denaturant, the fusion protein combined with plant oil body is quickly incorporated into making AOBs upon sonication. The AOBs-based system is characterized in simplicity as well as efficiency and is particularly useful for the immobilization of enzymes with low solubility.
TW94145952A 2005-12-22 2005-12-22 Immobilized proteins based on oil body TWI361833B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW94145952A TWI361833B (en) 2005-12-22 2005-12-22 Immobilized proteins based on oil body

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW94145952A TWI361833B (en) 2005-12-22 2005-12-22 Immobilized proteins based on oil body

Publications (2)

Publication Number Publication Date
TW200724687A true TW200724687A (en) 2007-07-01
TWI361833B TWI361833B (en) 2012-04-11

Family

ID=46727877

Family Applications (1)

Application Number Title Priority Date Filing Date
TW94145952A TWI361833B (en) 2005-12-22 2005-12-22 Immobilized proteins based on oil body

Country Status (1)

Country Link
TW (1) TWI361833B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011111456A (en) * 2009-11-25 2011-06-09 China Medical Univ Oil body carrier, use in target therapy and/or detection of the same, and fusion protein comprised therein
CN112980858A (en) * 2019-12-13 2021-06-18 中国科学院天津工业生物技术研究所 Technology for producing inositol based on immobilized multienzyme prepared by artificial oil body
WO2022213721A1 (en) * 2021-04-07 2022-10-13 中国科学院天津工业生物技术研究所 Method for producing tagatose by immobilizing multiple enzymes by using artificial oil body

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011111456A (en) * 2009-11-25 2011-06-09 China Medical Univ Oil body carrier, use in target therapy and/or detection of the same, and fusion protein comprised therein
CN112980858A (en) * 2019-12-13 2021-06-18 中国科学院天津工业生物技术研究所 Technology for producing inositol based on immobilized multienzyme prepared by artificial oil body
CN112980858B (en) * 2019-12-13 2023-09-08 中国科学院天津工业生物技术研究所 Technology for preparing immobilized multienzyme to produce inositol based on artificial oil
WO2022213721A1 (en) * 2021-04-07 2022-10-13 中国科学院天津工业生物技术研究所 Method for producing tagatose by immobilizing multiple enzymes by using artificial oil body

Also Published As

Publication number Publication date
TWI361833B (en) 2012-04-11

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