TW200724687A - Immobilized proteins based on oil body - Google Patents
Immobilized proteins based on oil bodyInfo
- Publication number
- TW200724687A TW200724687A TW094145952A TW94145952A TW200724687A TW 200724687 A TW200724687 A TW 200724687A TW 094145952 A TW094145952 A TW 094145952A TW 94145952 A TW94145952 A TW 94145952A TW 200724687 A TW200724687 A TW 200724687A
- Authority
- TW
- Taiwan
- Prior art keywords
- aobs
- oil body
- proteins based
- immobilization
- immobilized proteins
- Prior art date
Links
Abstract
The present invention is related to a new method based on artificial oil bodies (AOBs) was proposed to achieve protein refolding and immobilization in one step. Without adding denaturant, the fusion protein combined with plant oil body is quickly incorporated into making AOBs upon sonication. The AOBs-based system is characterized in simplicity as well as efficiency and is particularly useful for the immobilization of enzymes with low solubility.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW94145952A TWI361833B (en) | 2005-12-22 | 2005-12-22 | Immobilized proteins based on oil body |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW94145952A TWI361833B (en) | 2005-12-22 | 2005-12-22 | Immobilized proteins based on oil body |
Publications (2)
Publication Number | Publication Date |
---|---|
TW200724687A true TW200724687A (en) | 2007-07-01 |
TWI361833B TWI361833B (en) | 2012-04-11 |
Family
ID=46727877
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW94145952A TWI361833B (en) | 2005-12-22 | 2005-12-22 | Immobilized proteins based on oil body |
Country Status (1)
Country | Link |
---|---|
TW (1) | TWI361833B (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011111456A (en) * | 2009-11-25 | 2011-06-09 | China Medical Univ | Oil body carrier, use in target therapy and/or detection of the same, and fusion protein comprised therein |
CN112980858A (en) * | 2019-12-13 | 2021-06-18 | 中国科学院天津工业生物技术研究所 | Technology for producing inositol based on immobilized multienzyme prepared by artificial oil body |
WO2022213721A1 (en) * | 2021-04-07 | 2022-10-13 | 中国科学院天津工业生物技术研究所 | Method for producing tagatose by immobilizing multiple enzymes by using artificial oil body |
-
2005
- 2005-12-22 TW TW94145952A patent/TWI361833B/en active
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011111456A (en) * | 2009-11-25 | 2011-06-09 | China Medical Univ | Oil body carrier, use in target therapy and/or detection of the same, and fusion protein comprised therein |
CN112980858A (en) * | 2019-12-13 | 2021-06-18 | 中国科学院天津工业生物技术研究所 | Technology for producing inositol based on immobilized multienzyme prepared by artificial oil body |
CN112980858B (en) * | 2019-12-13 | 2023-09-08 | 中国科学院天津工业生物技术研究所 | Technology for preparing immobilized multienzyme to produce inositol based on artificial oil |
WO2022213721A1 (en) * | 2021-04-07 | 2022-10-13 | 中国科学院天津工业生物技术研究所 | Method for producing tagatose by immobilizing multiple enzymes by using artificial oil body |
Also Published As
Publication number | Publication date |
---|---|
TWI361833B (en) | 2012-04-11 |
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