WO2008015419A3 - Protein solubilisation - Google Patents

Protein solubilisation Download PDF

Info

Publication number
WO2008015419A3
WO2008015419A3 PCT/GB2007/002914 GB2007002914W WO2008015419A3 WO 2008015419 A3 WO2008015419 A3 WO 2008015419A3 GB 2007002914 W GB2007002914 W GB 2007002914W WO 2008015419 A3 WO2008015419 A3 WO 2008015419A3
Authority
WO
WIPO (PCT)
Prior art keywords
solution
protein solubilisation
protein
solubilisation
maintaining
Prior art date
Application number
PCT/GB2007/002914
Other languages
French (fr)
Other versions
WO2008015419A2 (en
Inventor
Paul Fyfe
Original Assignee
Univ Dundee
Paul Fyfe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Dundee, Paul Fyfe filed Critical Univ Dundee
Priority to GB0902200A priority Critical patent/GB2453901B/en
Publication of WO2008015419A2 publication Critical patent/WO2008015419A2/en
Publication of WO2008015419A3 publication Critical patent/WO2008015419A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a method of identifying optimal or desirable conditions for maintaining a selected protein in solution for enabling proteins to be kept in solution at high enough concentrations for structural analysis and the like.
PCT/GB2007/002914 2006-08-02 2007-08-01 Protein solubilisation WO2008015419A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB0902200A GB2453901B (en) 2006-08-02 2007-08-01 Protein solubilisation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0615312.6 2006-08-02
GBGB0615312.6A GB0615312D0 (en) 2006-08-02 2006-08-02 Protein Solubilisation

Publications (2)

Publication Number Publication Date
WO2008015419A2 WO2008015419A2 (en) 2008-02-07
WO2008015419A3 true WO2008015419A3 (en) 2008-03-20

Family

ID=37027070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/002914 WO2008015419A2 (en) 2006-08-02 2007-08-01 Protein solubilisation

Country Status (2)

Country Link
GB (2) GB0615312D0 (en)
WO (1) WO2008015419A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100014674A (en) 2007-03-29 2010-02-10 아보트 러보러터리즈 Crystalline anti-human il-12 antibodies
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
US8557253B2 (en) * 2009-10-07 2013-10-15 Sanofi Pasteur Sa Stabilizing excipient for inactivated whole virus vaccine
CN107290545B (en) * 2017-06-19 2019-01-18 威海威仕泰医疗科技有限公司 A kind of stable β2-microglobulin solution, preparation method and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017526A1 (en) * 2003-08-05 2005-02-24 The Research Foundation Of State University Of New York Reconstitution medium for protein and peptide formulations

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017526A1 (en) * 2003-08-05 2005-02-24 The Research Foundation Of State University Of New York Reconstitution medium for protein and peptide formulations

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "EZQ Protein Quantitation Kit (R33200)", MOLECULAR PROBES - INVITROGEN DETECTION TECHNOLOGIES, 8 July 2005 (2005-07-08), pages 1 - 4, XP002463960, Retrieved from the Internet <URL:http://wolfson.huji.ac.il/purification/PDF/Protein_Quantification/MOLECULAR_PROBES_EZQProtQuantKit.pdf> [retrieved on 20080110] *
BONDOS S E ET AL: "Detection and prevention of protein aggregation before, during, and after purification.", ANALYTICAL BIOCHEMISTRY, vol. 316, no. 2, 15 May 2003 (2003-05-15), pages 223 - 231, XP009094194, ISSN: 0003-2697 *
JANCARIK J ET AL: "Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins.", ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY, vol. 60, no. Pt 9, September 2004 (2004-09-01), pages 1670 - 1673, XP009094193, ISSN: 0907-4449 *
KAWATE T ET AL: "Fluorescence-Detection Size-Exclusion Chromatography for Precrystallization Screening of Integral Membrane Proteins", STRUCTURE, CURRENT BIOLOGY LTD., PHILADELPHIA, PA, US, vol. 14, no. 4, April 2006 (2006-04-01), pages 673 - 681, XP005387802, ISSN: 0969-2126 *
LE MAIRE M ET AL: "Interaction of membrane proteins and lipids with solubilizing detergents", BIOCHIMICA ET BIOPHYSICA ACTA - BIOMEMBRANES, vol. 1508, no. 1-2, 23 November 2000 (2000-11-23), NETHERLANDS, pages 86 - 111, XP004273339, ISSN: 0005-2736 *
WELLING-WESTER S ET AL: "Effect of detergents on the structure of integral membrane proteins of Sendai virus studied with size-exclusion high-performance liquid chromatography and monoclonal antibodies.", JOURNAL OF CHROMATOGRAPHY, vol. 443, 29 June 1988 (1988-06-29), pages 255 - 266, XP009094232, ISSN: 0021-9673 *

Also Published As

Publication number Publication date
GB2453901A (en) 2009-04-22
GB0902200D0 (en) 2009-03-25
GB2453901B (en) 2011-07-13
GB0615312D0 (en) 2006-09-13
WO2008015419A2 (en) 2008-02-07

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