TW200716663A - Oil body-based purification of proteins - Google Patents

Oil body-based purification of proteins

Info

Publication number
TW200716663A
TW200716663A TW094136100A TW94136100A TW200716663A TW 200716663 A TW200716663 A TW 200716663A TW 094136100 A TW094136100 A TW 094136100A TW 94136100 A TW94136100 A TW 94136100A TW 200716663 A TW200716663 A TW 200716663A
Authority
TW
Taiwan
Prior art keywords
proteins
oil body
protein
based purification
hybrid molecules
Prior art date
Application number
TW094136100A
Other languages
Chinese (zh)
Other versions
TWI304810B (en
Inventor
Chung-Jen Chiang
Hung-Chen Chen
Yun-Peng Chao
Tze-Cheng Tzen
Original Assignee
Nat Univ Chung Hsing
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Univ Chung Hsing filed Critical Nat Univ Chung Hsing
Priority to TW94136100A priority Critical patent/TWI304810B/en
Publication of TW200716663A publication Critical patent/TW200716663A/en
Application granted granted Critical
Publication of TWI304810B publication Critical patent/TWI304810B/en

Links

Abstract

The present invention provides a method of preparing proteins, and in particularly, a process for producing proteins by expression of hybrid molecules composed of the protein of interest and an oil body-binding protein, purifying the hybrid molecules and then separating the protein of interest from the rest of the hybrid molecules. Furthermore, the self-splicing intein used in the hybrid molecule of the present invention facilitates the purification and lowers the cost.
TW94136100A 2005-10-17 2005-10-17 Oil body-based purification of proteins TWI304810B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW94136100A TWI304810B (en) 2005-10-17 2005-10-17 Oil body-based purification of proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW94136100A TWI304810B (en) 2005-10-17 2005-10-17 Oil body-based purification of proteins

Publications (2)

Publication Number Publication Date
TW200716663A true TW200716663A (en) 2007-05-01
TWI304810B TWI304810B (en) 2009-01-01

Family

ID=45071023

Family Applications (1)

Application Number Title Priority Date Filing Date
TW94136100A TWI304810B (en) 2005-10-17 2005-10-17 Oil body-based purification of proteins

Country Status (1)

Country Link
TW (1) TWI304810B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115074340A (en) * 2022-06-17 2022-09-20 广州市乾相生物科技有限公司 Novel intein and application thereof in synthesis of human tropoelastin

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115074340A (en) * 2022-06-17 2022-09-20 广州市乾相生物科技有限公司 Novel intein and application thereof in synthesis of human tropoelastin
CN115074340B (en) * 2022-06-17 2023-02-28 广州市乾相生物科技有限公司 Novel intein and application thereof in synthesis of human tropoelastin

Also Published As

Publication number Publication date
TWI304810B (en) 2009-01-01

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Legal Events

Date Code Title Description
MM4A Annulment or lapse of patent due to non-payment of fees