TW200536486A - Treated ganoderma lucidum and manufacturing method of processed product of ganoderma lucidum - Google Patents

Abstract

This invention provides a manufacturing method of treated Ganoderma lucidum, characterized by treating the Ganoderma lucidum with water or steam at 150~210 DEG C; and a manufacturing method of processed product of Ganoderma lucidum, characterized by processing the above treated Ganoderma lucidum through the following steps: (1) cutting or pulverizing said treated Ganoderma lucidum, (2) extracting with an aqueous solvent, and/or (3) subjecting the extract obtained from step (2) to concentration or drying to powder. According to this invention, treated Ganoderma lucidum and processed product of Ganoderma lucidum can be prepared efficiently and thus is suitable for industrial use. Furthermore, said treated Ganoderma lucidum or processed product of Ganoderma lucidum have excellent anti-tumor effect and hence is suitable for use as a medicine, a food or a drink.

Description

200536486 IX. Description of the invention: [Technical field to which the invention belongs] The present invention relates to a method for manufacturing Ganoderma lucidum processed products or processed products of Ganoderma lucidum and food and beverages and pharmaceutical products containing such processed products or processed products. [Prior art] Hot water extracts of mushrooms have been rumored to have various medicinal effects since ancient times. In particular, mushrooms effective for cancer are known in China and Japan. Science 1 is also supporting and reporting this. For example, the non-patent document Series I reveals that hot water extracts of edible mushrooms have host: in vivo antitumor activity against SareQma 18G cancer-bearing mice. Among the edible tadpoles, Ganoderma lucidum is also known as Wanlong Velvet; and Ganodema lucidum has been known since ancient times: With ::: Medicine effect, especially for cancer two mushrooms, two patents, two non-sugars, m ~ The active ingredient is multi-species' / 3-D-glucan. Ganoderma usually uses 100. . The following hot water extraction is because of this method, but the extraction efficiency is very poor, so it is an extraction efficiency method in recent years. The research and development of the Chimori system can improve the quality. J Document 1 describes the use of pressurized hot water extraction and steam heat treatment. In terms of the extraction of the stroke extract, Patent Document 2 describes that it has the action of the ancient fu-α-futian pressurized hot water treatment 1 and the extract 2NKK activity J from the south, and further, I 316979 5 200536486 θ 1 '' ', Take the leech, and then carry out saturated water vapor pressure treatment, and pay the extract with active oxygen elimination activity as the main purpose. Furthermore, Patent Document 3 describes that after the mushrooms are pulverized by ultrasound, the cellulose peptone is used to process the raw materials, and the main purpose is to increase the amount of the carbohydrate.卞: The above patent documents 1 to 3 do not fully confirm the antitumor effect, and are concerned with the processing technology of monolingzhi to obtain a composition capable of exhibiting high antitumor activity. (Patent Document 1) Japanese Patent Laid-Open Publication No. U-19681 No. 8 (Patent Document 2) Japanese Patent Laid-Open Patent No. 200187 187745 (Patent Literature 3) Japanese Patent Laid-Open Publication No. 2000-26282 Non-Patent Document 1) jpn j Cancer Res 59, 155, 1968 (Non-Patent Document 2) Agnc · Biol. Chem., 49, 2641, 1985 [Summary of the Invention] The object of the present invention is to provide good extraction efficiency and industrial advantage A method for producing a processed or processed Ganoderma lucidum with excellent antitumor effect. Furthermore, an object of the present invention is to provide a food or drink or a medicament containing the aforementioned processed or processed ganoderma. The inventors of the present case repeated the research results in order to achieve the above-mentioned purpose, and found that the Ganoderma lucidum processed product and its processed product obtained by treating Ganoderma lucidum with 150 to 21 μC of water vapor contain more water-soluble 0-glucan than the conventional one, And has a strong anti-tumor effect. The inventors of this case repeated the in-depth discussion to complete the present invention. That is, the present invention relates to 316979 200536486 [1] A method for manufacturing a Ganoderma lucidum treatment product characterized by treating Ganoderma lucidum with water vapor at 150 to 210 ° C, "[2] treating the foregoing with water vapor at 180 ° C to 21 ° C [1] A method for producing a ganoderma treatment product, [3] The method for producing a ganoderma treatment product of the aforementioned [1] or [2] in which water vapor is saturated water vapor, [4] an oxygen concentration of 0 to 1 Mg / mL The method for manufacturing the Ganoderma lucidum processed material according to any one of the above-mentioned [1] to [3], which is processed below. Lu [5] Any one of the above-mentioned [1] to [4], which is subjected to steam treatment for 1 to 30 minutes * [6] A method for producing a Ganoderma lucidum treated product, [6] a Ganoderma lucidum treated product containing 0.5% by weight or more of water-soluble glucan. [7] Obtained by the production method according to any one of the above [1] to [5] The Ganoderma lucidum treated product of the above 6. [8] It is characterized in that the Ganoderma lucidum processed material obtained by treating the Ganoderma lucidum with water vapor at 150 to 210 ° C 1) is sheared or powdered, • 2) is extracted with an aqueous solvent, or 3) is The extraction solution obtained in the above 2) is further concentrated and refined or dried and powdered. [9] It is characterized by the method of extracting Ganoderma lucidum with water at 150 to 210 ° C, or further concentrating the water extract, refining or drying the powdered Ganoderma lucidum processed product. [10] The method for producing the processed Ganoderma lucidum of [9] described in [9], which is characterized by extracting water-soluble / 3-glucan at a ratio of 0.5% by weight or more of the raw material Ganoderma lucidum, 7 316979 200536486 [11] [8], [9] or [10], the processed ganoderma lucidum product obtained by the method of manufacturing [8], [9] or [7], or the processed ganoderma lucidum product of [11] Food and beverage, [13] Food and beverage with the above-mentioned [12] marked with anti-tumor effect, immune activation effect or anti-cancer effect, [14] Ganoderma treatment containing [6] or [7], Or the pharmaceutical product of the processed ganoderma lucidum of [11] above, and the pharmaceutical product of [I4] above that [I5] is an antitumor agent. '' According to the present invention, Ganoderma lucidum processed product or processed Ganoderma lucidum which has excellent extraction efficiency and industrially favorable anti-tumor effect is produced. Furthermore, according to the present invention, it is possible to provide a food or a medicine containing a Ganoderma lucidum treated product or a processed product of Ganoderma lucidum which is excellent in the aforementioned antitumor effect. [Embodiment] The Ganoderma lucidum used in the present invention is a fruiting body of Ganoderma lucidum (Fr.) Krast of polyporaceae. Ganoderma lucidum can also grow with the same species, according to temperature, humidity, luminosity, carbon dioxide concentration and other growth conditions, or according to the origin and system of the strain, it can cultivate fruit bodies (ganoderma lucidum) of different colors or shapes, classified by different colors There are types such as green, red, yellow, white, purple, and black, and they are classified into staghorn, oxhorn, yunzhi, meat and other types according to the shape. In the present invention, any of the above-mentioned ganoderma can be used, and a commercially available product called ganoderma or staghorn ganoderma can also be used as ganoderma. In the present invention, all the fruit bodies of Ganoderma lucidum can be used, or a part of the fruit bodies can be used (such as those that have been appropriately cut off) as 316979 200536486 • Beijing materials, and the above s usually means no water or organic solvents, etc. Treatment. In the present invention, the aforementioned ganoderma can be prevented from contacting water before the water vapor treatment is applied. 2. One aspect of the present invention is a person who processes Ganoderma lucidum with water vapor at 150 to 2 UTC to produce a Ganoderma lucidum treatment object. "In the present invention, the water vapor used for the treatment of ganoderma is preferably saturated water vapor from the point of workability and operability, but it is not limited to this. The processing conditions are as long as the water vapor temperature is 150i to 21 generations. It is not particularly limited. In the aspect of the present invention, it is particularly preferable that the temperature range is 170 to 21 ° (180 ° to 210 ° C). Since the water vapor temperature is 15G ° C, Increasing the effect will decrease the temperature and increase the effect of the aforementioned active ingredient to 2HTC, which is not ideal. The treatment time is not particularly limited so as not to hinder the object of the present invention, but it is 1 to 30 minutes. Limit.—In general, the higher the temperature of the water, the shorter the processing time; for example, if the temperature of the water is iron, the minutes are what we expect. It is particularly limited because of the concern about the influence of oxygen 4 ′ on the generation of organic acids or furfural due to oxidative decomposition, it is better to use a low oxygen concentration. For example, the gas concentration of / mL is what we expect. The aforementioned treatment can be performed at normal pressure. Proceed, but-generally It is said that it is pure under pressure. When it is performed under pressure, the pressure is not particularly limited. The pressure when using saturated water vapor as the water vapor is usually 03 316979 9 200536486 to 1.8 MPa. The water vapor treatment can be performed using a conventional treatment device. The treatment device is not particularly limited as long as it can perform water vapor treatment at 150 to 210 ° C. For example, a high-temperature and high-pressure reaction device manufactured by AKICO Corporation or Nisaka can be used. High-temperature wet heat treatment test equipment (HTS-25 / 140-8039) manufactured by Seisakusho Co., Ltd., Superheated steam generator (DPH GH20) manufactured by Seta Kosan Chemical Co., Ltd., etc. In the present invention, by The obtained Ganoderma lucidum processed material is further subjected to a conventional processing to produce a processed Ganoderma lucidum. For example, by processing the above-mentioned Ganoderma lucidum- 1) by cutting or powdering, 2) extracting with an aqueous solvent, 3) The extract solution obtained in the above 2) is further concentrated and refined or dried and powdered to produce processed Ganoderma lucidum. The treatment in any one of the above 1) to 3) can also be performed by a conventional method. ® Where the above 2) is used When manufacturing processed Ganoderma lucidum products, extraction operations can be performed by common methods as long as the extraction temperature, time, and equipment are appropriately selected. The aqueous solvents used as the extraction solvent in the above 2) treatment include aqueous solvents or water. In addition, it is also possible to use high-pressure greens to extract with hot water at, for example, 120 ° C. Therefore, a processed Ganoderma lucidum product is obtained as an extraction solution. In the case of processing the processed Ganoderma lucidum by using the process 3), for example, By removing the solvent of the extract obtained through the process of 2) above, it becomes a concentrated extract, or freeze drying or air drying can be used to make the extract 10 316979 200536486 The liquid can also be diluted with a solvent such as water and treated by 2) above.

One of the invention's processed products. Press water. It is extracted with water of 150 to ^ (^) in the present invention. The water used for the extraction of ganoderma in the present invention is expected to be made at high temperature and high temperature. The processing conditions are not specific as long as the temperature of the water is 150 to 2 HTC. The middle range is ⑽ to 2 ^ • car 乂 仏. As the temperature of the water is not & 15 0, the effect of increasing the anti-tumor activity component-min will be reduced, and it will also be higher than 20.0. The effect of reducing the increase of the aforementioned active ingredients is not ideal. Furthermore, the oxygen concentration in the aforementioned treatment is not particularly limited. Since oxygen can be used, organic acids or furfural can be generated due to oxidative decomposition, which can cause aroma generation. Concerns about the impact, so it is better to have a low oxygen concentration. For example, the oxygen concentration of 0 to i ^ / ml is what we expect. _, The treatment can be performed under normal pressure, but generally under pressure The process is preferably performed. When the process is performed under pressure, the pressurization pressure is not particularly limited, and the pressurization pressure is usually 0.3 to 1.8 MPa. The above-mentioned water treatment can be performed using a conventional treatment device. Allows high temperatures from 150 to 210 ° C The water extraction treatment under high temperature and high pressure is not particularly limited. For example, a high temperature and high pressure reaction device manufactured by AKICO or a high temperature wet heat treatment test device (HTS-25 / 140-803 9) manufactured by Nisaka Manufacturing Co., Ltd. can be used. Seta Superheated steam generator (DPH GH20), etc. manufactured by Xingchan Chemical Industry Co., Ltd. 316979 200536486 The present invention can use the extract solution extracted with high temperature and high pressure water as the processed product of Ganoderma lucidum. The obtained processed Ganoderma lucidum is further subjected to a conventional treatment. For example, the solvent of the extract solution can be removed to become a concentrated extract, or the aforementioned extract solution can be dried into a dry powder product by means of freeze-drying or air-drying. Furthermore, It can also be diluted with solvents such as water. These are also known as processed ganoderma. One aspect of the present invention is to treat ganoderma with water vapor at 150 to 210 ° C to produce ganoderma processed products. Or ganoderma processed products contain high levels of water-soluble glucans known to have antitumor effects. For example, It is impossible to extract water-soluble / 3-glucan less than 0.1 g from 100 g of ganoderma, and the water vapor treatment or high-temperature and high-pressure water treatment of the present invention can be performed. Each 100 g of raw ganoderma can provide 0.5 to 5 g of water-soluble dextran. Other aspects of the present invention are also foods and medicines containing the above-mentioned ganoderma processed products or the above-mentioned processed products of ganoderma lucidum. The processed ganoderma lucidum is manufactured by conventional means. Since the processed ganoderma and processed ganoderma have excellent safety, it is generally not necessary to use special treatment for obtaining safety and the like. In the food and drink and medicine of the present invention, The blending amount of the processed ganoderma and the processed processed ganoderma can be considered in consideration of its effect, and the blending amount of the product can be appropriately determined when adding the flavor and color of the product. However, a preferable specific range of the compounding amount is exemplified by the conversion of the above-mentioned ganoderma processed product or the processed ganoderma lucidum to dry weight. Generally, it is desired that it is from 0.001 to 99 9% by mass for food and drink 316979 200536486 'or pharmaceutical products. , Preferably 0.01 to 99.5 mass. /. Range. I'm good. Examples of the mouth include sugar, sugar bond, chewing gum, yogurt, ice desert, pudding, fruit; east, water goat, alcoholic beverage, tea beverage, kiss. Non-beverages, fruit juices, fruit juice drinks, carbonated beverages, cool drinks, milk, whey drinks, lactic acid bacteria drinks and other food and beverage products. The above 饫 5 ° σ, the processed ganoderma or processed ganoderma, and various additives as required can be manufactured according to a common method. Examples of additives to be blended when necessary include, for example, glucose, fructose, sucrose, maltose, sorbitol, stevia rebaudiana, rubusside, corn mash, lactose, bezel acid, tartaric acid, malic acid, succinic acid, Lactic acid, L-ascorbic acid, mim, sodium erythorbic acid (sodmm) such as glycerol, glycerol, glycerol, polyglycerol fatty acid ester, sucrose fatty acid ester, "sorbitan fatty acid g, propylene glycol fatty acid 3 purposes, gum arabic, carrageenan, casein, gelatin, pectin, agar, vitamin b, alkalamine, calcium pantothenate, amino acids, dance salts, pigments, spices, preservatives, etc. Those who use it as food ingredients. The above-mentioned foods and drinks can also be health foods. The shape of health foods is particularly limited. They can be made into granules, capsules, pills, tablets μ, extracts, pastes, and beverages. Or other shapes, such as leather, which can be extracted with hot water, etc. In the present invention, according to a common method, "Neng Yi" contains the above-mentioned ganoderma treatment or the above-mentioned spirit in an appropriate amount. lt; The knife edge is the second best product for the purpose. More specifically, 'For example, according to a common method, it can be appropriately matched with the processed product of ganoderma lucidum or the processed product of the above-mentioned ganoderma, and the desired plant: 316979 13 200536486, such as oils and fats, containing water Waxes such as lanolin or beeswax, hydrocarbons, fatty acids, higher alcohols, esters, various pigments, spices, vitamins, plant and animal extracts, antioxidants, preservatives, fungicides, etc. are commonly used as raw materials for health foods. The health food is obtained. The food and drink of the present invention can impart the effect known as β-glucan, for example, a marker having an antitumor effect, an immune activation effect, or an anticancer effect. The label position is listed on a container or attached to it The instructions include printing, printing, sealing, etc., but are not limited to this. Φ Furthermore, the food and beverage of the present invention has good antitumor effects from any of the above-mentioned ganoderma treatment and ganoderma. Processed products , Suitable for use as so-called health food, as a health food, especially suitable for use as an anti-tumor agent Food. The pharmaceutical product of the present invention can be formulated with the above-mentioned ganoderma lucidum processed product or the above-mentioned ganoderma processed product and various additives as required, and further contains a processed ganoderma lucidum product in an appropriate amount to prepare various pharmaceutical forms. Examples of the above-mentioned pharmaceutical products include lozenges , Capsules, powders, sugars, syrups, extracts and other oral medicines, or ointments, eye ointments, lotions, emulsions, patches, suppositories, eye drops, nose drops, injections and other non-oral medicines These medicines can be manufactured according to common methods by using the above-mentioned ganoderma processed products or the above-mentioned ganoderma processed products and various additives as required. The additives used can be well-known. Examples of the additives include starch, lactose, white sugar, mannitol, Carboxymethyl cellulose, corn starch, inorganic salts and other solid carriers; distilled water, physiological saline, glucose aqueous solution, alcohols such as ethanol, propylene glycol, polyethylene glycol and other liquid carriers; various animal and vegetable oils, Bai Fan 14 316979 200536486 Shihlin , Paraffin, wax and other oily carriers. There are no particular restrictions on the daily use, dosage, and amount of the Ganoderma lucidum processed product and the processed Ganoderma lucidum processed product. For example, it can be 0.01 mg to 100 g in terms of dry weight. In the case of an antitumor effect, for example, it is preferably 0.1 g to 20 g in terms of dry weight. The food and drink (especially health food) and medicines of the present invention can also be used as raw materials for other foods, health foods or medicines with anti-tumor effects, such as Agaricus, Maitake, Morus alba, etc. , Microbes containing anti-cancer polysaccharides such as / 3-glucose and sugar or their fermented products, polysaccharides (Krestin) ^ and other pharmaceutical products. When using the food, drink, medicine or additive of the present invention, the mixing ratio can be appropriately set according to the purpose, and is not particularly limited. The pharmaceutical product of the present invention is suitable for use as an antitumor agent in view of the good antitumor effect of any of the above-mentioned ganoderma treatment products and processed ganoderma products. ® Furthermore, it is suitable for oral medicine because of its excellent safety when used orally. Examples (Example 1-1) Antler ganoderma (commercial product, produced in Shandong Province, China) was used as a raw material. A high-temperature and high-pressure reactor manufactured by AKICO is used for high-temperature and high-pressure processing. 15 316979 200536486. A sus 3 1 6 alloy heat-resistant and pressure-resistant container (400 ml) was filled with 40 mi of water which was bubbled with an atmosphere to remove dissolved oxygen. Into a 200 mi monitor made of SUS 3 16 alloy, staghorn ganoderma lucidum cut into 1 cm pieces was placed, and these ganoderma were placed in a heat-resistant and pressure-resistant container made of sus 316 alloy without contact with water. The air in the container was replaced by nitrogen in the course of about 5 seconds, then at 150. (: Saturated water vapor, pressurized at a pressure of 038 ㈣ ml, water vapor treatment is performed for $ minutes. Then, when the reaction container becomes a thief, the container is opened, and the antlers-ganoderma lucidum treatment product obtained from the antlers is loaded Pressure-resistant bottle of wonderful water, in [2] T '7 π Eight Force' ,, ', 1L, 1C, 30 knives into the back of the staghorn ganoderma lucidum extract (ganoderma processed product) ， Take (-Pao Jinzu take), (Performance Example 1-2) Water vapor, 0.66 1 -1 Similarly, except that the water vapor treatment conditions are 17〇t MPa, and the oxygen concentration is pjQs /, Ding Pingchen. Degrees other than 0.95 Yang / ⑹, fish

To obtain staghorn ganoderma lucidum extract (ganoderma lucidum plus I product ;, shellfish) (Example 1 «3) except that the water vapor treatment conditions are 180. He, oxygen; agricultural degree is 0.95 ^ ml, water vapor, U5 application Antlers Ganoderma lucidum extract was obtained (spirit ... Example Η same as ―1- 'οσ) 〇 (consistent Example 1-4) Except for water vapor treatment conditions, the oxygen concentration was 095 Yang / C saturated water vapor, An antler extract of Ganoderma lucidum (Ganoderma Lucidum, Example 1-1 was performed in the same manner as 316979 200536486 (Example-5) except that the water stripe was treated at 210 ° C in saturated water vapor, and the concentration of 18 MPa was G. · 95 batches / except, in the same manner as in Example M, she obtained antlers ganoderma lucidum extract (ganoderma lucidum processed product). (Comparative Example 1-1) Except for water vapor treatment conditions of 22 (rc saturated water grave gas, 22

Except for Mpa and oxygen concentration of 0.95 / g / ml, staghorn lingzhi extract was obtained in the same manner as in Example M. ^ Only (Comparative Example 1-2) The water-removal treatment conditions were 250. Saturated water vapor of 〇, 4 · 5 a lice, the degree of 辰 95 places / Qiu, and Example [I] to obtain antlers Ganoderma lucidum extract. Ά (Comparative Example 1-3) In addition to the water vapor treatment conditions of 30 (rc of saturated water vapor, 9 Pa Pa 'oxygen concentration of 0.95 yg / mi, she served antlers Ganoderma lucidum extract. Month, (Comparative Example 1- 4) Without water vapor treatment, put staghorn ganoderma into a pressure-resistant bottle, 22: bu at 121 ..., 3 minutes of extraction (high pressure Cang take) to obtain staghorn ganoderma extract. (4 Test Example 1) Evaluation of the antitumor activity of the antlers ganoderma lucidum extract obtained in the above-mentioned Examples w, Example i_3, and P_5, and Comparative Examples U to 1-4, was evaluated by treating the tumours of the processed tumors with high temperature and high pressure water vapor. 316979 17 200536486 C3H mice (Charles River, Japan) were subcutaneously inoculated with 2 × 10 06 cells of MM46 breast cancer cells, and the mice (hereinafter referred to as MM46 cancer-bearing mice) were used as subjects. Subcutaneous inoculation10. After 12 and 14 days, the dried dried antlers of Ganoderma lucidum extract obtained in the above Examples 1-1, Examples 1-3 to I-5, and Comparative Examples 1-2 to 1-4 were dissolved in physiological saline at The rats were administered in the abdominal cavity in a 250-row manner. Furthermore, MM administered with only the same amount of physiological saline was used. 46 Luo cancer milk was used as a control group. The tumor weight was determined 28 days after subcutaneous inoculation, and the tumor weight of each mouse was compared when the tumor weight of the control mice was 100%. The measurement results are shown in Table 1.

\ I 'and administration of the antler ganoderma lucidum extract of Comparative Example 丨 -4 when the second substance = :, can determine the antitumor activity of the antler ganoderma lucidum extract of Example T when administered, compared with the antitumor activity of the black extract Phase: Antler ganoderma lucidum of comparative example 1-3 of the administration example of antler ganoderma lucidum X: The antitumor activity was low when discriminating the administration of comparative example 丨 -2 and one of the samples. This can be attributed to the progress of decomposition of the active ingredient. (Example 2-1) ‘Antlers Ganoderma (commercial product, produced in Shandong Province, China) was used as a raw material. A high-temperature and high-pressure reactor manufactured by KICO was used for high-temperature and high-pressure processing. A heat-resistant and pressure-resistant container (400 ml) made of SUS 316 alloy was charged with 230 ml of water to remove dissolved oxygen to an oxygen concentration of 0.95 / ig / ml or less by bubbling nitrogen. In a 200 ml basket made of SUS 316 alloy, 10 g of staghorn ganoderma lucidum cut into 1 cm pieces was placed, and these ganoderma were placed in a SUS 3 16 alloy heat-resistant and pressure-resistant container. After the air in the container was replaced with nitrogen gas for about 5 seconds, the temperature was maintained at 150 ° C and the pressure was treated at 0.3 8 MPa for 5 minutes. Next, the container was cooled, and when the reaction container became 80 ° C, the container was opened, and the staghorn lingzhi extract was recovered. This extract was dried to obtain Ganoderma lucidum processed product (lyophilized product). (Example 2-2) A staghorn lucid ganoderma extract was obtained in the same manner as in Example 2-1, except that the processing conditions were saturated water vapor at 180 ° C and spring at 0.85 MPa. This extract was lyophilized to obtain a processed Ganoderma lucidum (lyophilized product). (Example 2-3) A staghorn lucid ganoderma extract was obtained in the same manner as in Example 2-1, except that the processing conditions were saturated water vapor at 210 ° C. and 1.4 MPa. This extract was lyophilized to obtain a processed Ganoderma lucidum (lyophilized product). (Experimental Example 2) Antitumor activity of Ganoderma lucidum processed product obtained by extraction with high temperature and high pressure water 19 316979 200536486 to 2-0 Antitumor activity of Ganoderma lucidum processed product * " Evaluation of the effect of the above-mentioned Example 2 -1. As the subject, a MM46 Luo cancer mouse shown in Test Example i was used. After 10, 2, and 14 days of subcutaneous inoculation, the processed Ganoderma lucidum products obtained in the above Examples 2 to 2-3 were dissolved in a physiological saline solution and administered to each abdominal cavity. Furthermore, ^ M46 cancer-free mice administered only with the same amount of physiological salt were used as a control group. Tumors were removed 28 days after skin T inoculation. The tumor weight was measured and the tumor weight of the control mice was taken as 100%, and the tumor weight of each mouse was compared. The measurement results are shown in Table 2. ^ Compared to Comparative Examples 1-4 (untreated product), it can be discriminated that any of the processed stem lenticular products of the present invention has an excellent antitumor effect. 【Table 2]

(Experimental example 3) Extraction rate of processed Ganoderma lucidum extract and comparative examples ^ 1 to ~ staghorn ganoderma total 4: ratio: The yield is a graph showing the weight of staghorn ganoderma extract. It can be discriminated: = mass percentage of =. The measurement results are shown in the first comparative example. 1 ... what is the extraction rate 316979 20 200536486 ^ 4) Soluble glucan from Witch ’s processed products f-soluble glucan is known as one of the antitumor ingredients. The reason is that the dissolving amount of the water-soluble dextran of the Ganoderma lucidum second-hand product of Examples 1-4 and 2-2, which are particularly strong in antitumor effect, is analyzed. The dissolution of the 0-glucan from the extract of Ganoderma lucidum in the comparative example was analyzed. Τ _ knife analysis mouth mouth is using 1 g of antlers Ganoderma lucidum extract dissolved in 40 ml, * analysis by enzyme method according to common methods. In other words, the analysis sample port was reacted with Termamyl (R) in a phosphate buffer of 0.08 IV [, and then adjusted to pH 7 · 5 with a hydrazone / syringe and reacted with a protease. It was then adjusted to pH 4.3 with hydrochloric acid and reacted with starch transglucosidase. After adding * times the amount of 95, B to obtain Shen Dianwu (crude glycan division). This Shen Dianwu was washed with alcohol followed by acetone, neutralized with a force ruler in a boiling bath containing sulfuric acid and water, and filtered. The amount in the filtrate was quantified by the glucose oxidase method (using Wako = Glucose CII Wak〇). glucose. The content of water-soluble ^ -glucan contained in the extract obtained from 100 g of antlers is shown in FIG. 2. From Test Example 4, it can be clearly discriminated that the processed Ganoderma lucidum (Ganoderma lucidum extract) obtained by the method of Example 丨 _4 and Example 2_2 yields more water-cooled = / 3-glucan. In particular, as shown in the implementation of Mj_4, it can be judged that the hot water extraction method after treating Ganoderma with saturated water radon gas can effectively dissolve and dissolve water-soluble glucan. According to Test Examples 1 to 4, it can be discriminated that by treating Ganoderma lucidum with water or steam of 150 to 21 (Γ (:), not only can the extraction rate be improved, but also it can effectively dissolve 316979 200536486 and precipitate water-soluble / 3-glucan. Ganoderma processed product with enhanced anti-tumor effect ° — (Example 3) A high-temperature wet heat treatment test device (HTS-25 / 140-8039 manufactured by Hansaka Manufacturing Co., Ltd.) was used for high-temperature water vapor treatment, and a steam boiler (Miura Industrial (stock) manufacturing: FH-100). A 12L basket made of SUS 316 alloy was loaded with 1.5 kg of staghorn lingzhi used in Example 1-1 cut into 1 cm pieces. This basket was set on SUS 316 alloy A heat-resistant and pressure-resistant container (30 L) made of aluminum was sealed, and it was fed into the water (oxygen concentration of 0.3) using a deoxidizer (DOR-1000P, manufactured by Miura Industrial Co., Ltd .: DOR-1000P) in about 1 second. " g _ / ml) after replacing the air in the container with high-temperature, high-pressure saturated steam, and maintained at 200 ° C and a pressure of 1.4 MPa for 300 seconds. After degassing, the reaction container became Open the container below 80 ° C. Next, deer Ganoderma lucidum treated with 24 L of water was added twice at 121 ° C for 30 minutes (high pressure Cang extraction). The obtained extract was lyophilized to obtain dried staghorn ganoderma lucidum powder. ® (Test Example 5) dried staghorn ganoderma lucidum Antitumor effect of oral ingestion of powder In an example of a rat and rat standard feed AIN-93M (manufactured by Riental Yeast Industry Co., Ltd.), examples were mixed at a ratio of 0.156% by mass, 0.625% by mass, and 2.5% by mass, respectively. 2 The obtained antlers ganoderma lucidum dry powder was obtained into 3 kinds of mixed feeds (hereinafter referred to as 0.156% antler ganoderma lucidum food, 0.625% antler ganoderma lucidum food, 2.5% antler ganoderma lucidum food). Untreated antler ganoderma lucidum (commercial product, Shandong, China) (Produced by the province), pulverized, and mixed the antlers ganoderma powder at a ratio of 2.5% with the rat and rat standard 316979 200536486 feed AIN-93M (〇iieiital yeast (hereinafter referred to as 2.5% control food) as the three kinds of preparation The anti-tumor effects of staghorn ganoderma foods and products are discussed below. Prepared as a feed control in industrial (stock) manufacturing. The staghorn ganoderma lucidum with 2.5% of the control food can be used freely. ddY rats (Japanese SLC (stock) were divided into $ groups (10 per group), so that ddY rats were free to ingest only the three kinds of antler ganoderma lucidum food prepared above, 2.5% control food and standard feed AIN for rats and rats 93M (hereinafter referred to as food for control and group). After 1 week of breeding, 1 × 10 6 cells of Sarcoma80 sarcoma cells were inoculated subcutaneously in the chest of ddY mice as Sarcomal80 cancer-bearing mice. Continue to give 3 kinds of antler ganoderma lucidum food prepared above, 2 5% to J3, 、, food and 卩 卩, group food to feed Sarcoma 180 cancer mice. Eighteen days after subcutaneous implantation, the tumors were removed and their weights were measured. When the tumor weight of the control mice was taken as 100%, the tumor weight of each mouse was compared. The measurement results are shown in Table 3. [Table 3] Tumor weight (% of control group) 0.156% antler ganoderma food 62.7 0.625% antler ganoderma food 62.1 2.5% antler ganoderma food 46 — 2.5% control food 75T5 ^ From the table 3, there are three types In the case of staghorn ganoderma food, the antitumor activity increases with concentration. Furthermore, the antitumor activity of three kinds of antlers ganoderma lucidum food was shown to be stronger than that of 2.5% control food and control food Risi t 0 316979 23 200536486 ganoderma lucidum plus one product can be identified through Oral use also gives higher antitumor activity than untreated Ganoderma. (Experimental Example 6) The antitumor effect of Ganoderma lucidum treated with high temperature and high water vapor through oral ingestion is known only for the antlers Ganoderma lucidum used in Example M and Example 3 (water vapor treatment strip ^ :, 2 ′ ′, plus a force of h4MPa, 5 minutes) The same high temperature Wenbao Shuiluo gas treatment is carried out in the same way, not old ^ tired of tiredness + into the autoclave autoclave, dried, pulverized, and mixed with the standard feed for rats and rats at a ratio of 0.625 berry%. AIN-93M (Oriental Yeast Industry Co., Ltd.) is prepared into staghorn ganoderma lucidum food (hereinafter referred to as 0.625% staghorn ganoderma lucidum food). The staghorn ganoderma lucidum powder (commercial product, produced in Shandong Province, China) was crushed at a ratio of 2.5% to the standard feed for rats and rats AIN-93M (manufactured by 〇ientent Yeast Industry Co., Ltd.) and prepared into feed. (Hereinafter referred to as 25% control food) as a control. Use some food to explore the antitumor effect as follows. C3h rat (Japan

CharleS RlVer (shares) was divided into 5 groups (I5 per group), so that C3H rats were free to ingest only the above-prepared 0.625% staghorn ganoderma lucidum food, 2 5% control food, standard feed for rats and rats 93M (hereinafter referred to as the control food). After 1 week of rearing, MM46 breast cancer cells of 2 × 10 6 cells were inoculated subcutaneously in the rat's neck to obtain MM46 cancer-bearing mice. Twenty days after the subcutaneous inoculation, the tumor was removed and the weight of the tumor was measured. When the tumor weight of the control mouse was taken as 100%, the tumor weight of each mouse was compared. The measurement results are shown in Table 4. 316979 24 200536486 [Table 4] Weight (ΐΠϋZ ^ ζγ 64 ^ 6

Wm ^ K Disk 2 Table 4 Look at the comparison of the antlers and ganoderma lucidum food (2.5% control food) of the U25% antler ganoderma lucidum food can be discriminated according to the present invention, showing that the mixing amount of 1/4 is still stronger. Antitumor effect. In other words, it can be discerned that the treatment of staghorn stalks by high temperature and high water vapor treatment can improve the antitumor activity. · (Experiment Example 7) Treatment of staghorn lucid ganoderma with high temperature and high friction and anti-cancer effect _ with effect W J goods. Do not use lentinan or dagger of pharmaceuticals alone with anticancer polysaccharides derived from mushrooms and use with anticancer agents. Therefore, the combined effects of the high-pressure and high-pressure-treated antlers ganoderma lucidum extract and the anticancer & therapeutic agent of the present invention are discussed. C3H mice were subcutaneously inoculated with 2x106 cells of mm46 breast cancer cells subcutaneously as MM46 cancer-bearing mice. After i week of subcutaneous inoculation, the group was divided into 6 groups based on those with large tumors. 0.1 56% staghorn ganoderma lucidum food, 0.625% staghorn ganoderma lucidum food, 2.5% staghorn ganoderma lucidum food, and 25% 5% control food and control group food were freely ingested. Thirteen days after subcutaneous inoculation, the anticancer chemotherapy agent cyclophosphamide (CY) was administered to the abdominal cavity at 50 mg / kg. The control group was fed with control group food, and the same amount of physiological saline instead of CY was administered into the abdominal cavity. After 28 days from subcutaneous implantation, the tumors were removed and their weights were measured. The tumor weights of the control mice were taken as 100% and the tumor weights of each mouse were compared. The measurement results are shown in Table 5. 316979 25 200536486 [Table 5] Tumor weight (° / 〇 in control group) 0.156% Antler Ganoderma food (CY administration) 64.7 0.625% Antler Ganoderma food (CY administration) 50.9 2.5% Antler Ganoderma food (CY administration) 47.3 2.5% control food (CY administration) 60.5 Control food (CY administration) 64.8 Control group 100 According to Table 5, the φ group ingesting 3 kinds of antler ganoderma lucidum food or 2.5% control food was more significant than the CY group alone Inhibit tumor proliferation. Furthermore, in the case of comparing three kinds of antler ganoderma lucidum food with 2.5% control food, _ can also be judged that ingesting 0.625% antler ganoderma lucidum food and 2.5% antler ganoderma lucidum food can obtain a stronger antitumor effect. Furthermore, based on these results, it can be determined that the processed Ganoderma lucidum product of the present invention can be used in combination with other anticancer agents. Furthermore, compared with the conventional staghorn ganoderma lucidum processed product of the present invention, it can be judged that the combined effect with an anticancer chemotherapeutic agent is high. (Example 4) ® Bonding agent ... A tablet containing a processed product of ganoderma was produced by the method shown below. For high-temperature water vapor treatment, a high-temperature wet heat treatment test device (Saidaka Co., Ltd .: HTS-25 / 140-8039), and a steam boiler (Miura Industrial Co., Ltd .: FH-100) were used. In a 12L basket made of SUS 316 alloy, 1.5 kg of staghorn lucid ganoderma cut into 1 cm pieces was placed and sealed in a heat-resistant and pressure-resistant container (30 L) made of SUS 316 alloy. In approximately 1 second, it is sent to a high-temperature, high-pressure saturated vapor replacement vessel using 26 316979 200536486 water (oxygen concentration 0.3 μ§ / ml) deoxidized by a deoxidizer (Muraura Industrial Co., Ltd .: DOR-1000P). After the air, it is held at 200 ° C for 300 seconds under a high temperature and high pressure state of 1.4 MPa. After degassing, open the container when the reaction container becomes below 80 ° C. Next, 24 L of water was added to the steam treatment of the antler ganoderma obtained above, and extraction was performed twice at 121 ° C for 30 minutes (high pressure flea extraction) to obtain an antler ganoderma extract. This extract was lyophilized to obtain about 450 g of Ganoderma processed product A. 6 6 · 7 mg of this Ganoderma Lucidum processed product A, 23 2 · 0 mg of lactose, and 1 · 3 φ mg of magnesium stearate were mixed at the same time. A bond with a diameter of 10 mm and a weight of 300 mg. (Example 5) Extract: The staghorn ganoderma extract obtained in the same manner as in Example 3 was concentrated to 10% (W / W) brix to obtain a concentrated extract (processed Ganoderma lucidum B). This Ganoderma lucidum processed product B was diluted 10 times with sterile water and put into a bag. (Example 6) Lu granules 1 ··················································································· granules of mesh. (Example 7) Granule 2: The steam-treated product of staghorn lucid ganoderma that was subjected to high-temperature and high-pressure steam treatment in the same manner as in Example 3 was dried without adding water or high-pressure extraction. 27 316979 200536486 The material was powdered to a size of 200 mesh to prepare Ganoderma lucidum processed product C. 0.4 g of 0.1 g ^ of starch was added to 1.0 g of this Ganoderma processed product to obtain granules of 20 to 50 mesh. (Example 8) Ganoderma lucidum extract beverage (ganoderma tea beverage) 200 g of the processed ganoderma lucidum product C obtained in Example 6 was subjected to dropwise extraction with 5 L of water (90 ° C). After the obtained extract was centrifuged and filtered, 1.7 g of vitamin C, 3.4 g of sodium bicarbonate and water were added to make 17 L. After the resulting mixed solution was brought to a temperature of about 85 ° C., it was filled in a 190 g jar and sterilized by a distillation tank (125 .t, 14 minutes) to obtain 20 bottles of ganoderma extract beverage (ganoderma tea beverage). Result. Ganoderma lucidum extract is labeled with "anti-tumor food". (Example 9) Each of Ganoderma processed product A obtained in Example 3, Ganoderma processed product B obtained in Example 4, and Ganoderma processed product C obtained in Example 5 was used according to the following composition and manufactured according to a common method. Various foods and drinks mixed with processed ganoderma. (Example 9-1) Sugar: [Table 6] Composition_weight parts Sorbitol powder 99 ^ 7 ~~ Spice 0.2 Ganoderma processed product B 0.05 Sorbitol seed 0.05 Total weight 100.00 28 316979 200536486

(Example 9-2) Candy: [Table 7] Composition weight parts Granulated sugar 47.0 Maltose 49.76 Spice 1.0 Water 2.0 Ganoderma processed product 0.2 0.2 Total weight 100.00 (Example 9-3) Sugar lozenge: [Table 8] Composition weight parts Arab Gum 6.00 Glucose 73.00 Ganoderma lucidum processed product A 0.05 Dipotassium hydrogen phosphate 0.20 Carnic acid ~ monoazine 0.10 Lactose 17.00 Spice 0.10 Magnesium stearate 3.55 Total weight 100.00 (Example 9-4) Chewing gum: 29 316979 200536486 [Table 9 ] Composition parts by weight. Chewing gum base calcium carbonate Stevia chinensis Ganoderma processed product C Lactose flavor 20.00 2.00 0.10 0.05 76.85 1.00 Total weight 100.00 (Example 9-5) 'Jelly (coffee jelly): [Table 10]-Composition weight parts refined Fine sugar gelatin coffee extract Water Ganoderma Lucidum processed product B 15.00 1.00 5.00 78.93 0.07 Total weight • (Example 9-6) Water goat's gizzard: 100.00 30 316979 200536486 [Table 11] Composition weight parts of red shengming 24.80 agar powder 0.30 salt 0.10 on Equivalent sugar 24.90 Ganoderma processed product B 0.10 Water 49.80 Total weight 100.00 (Example 9-7) Juice = [Table 12] Composition weight parts Frozen concentrated Wenzhou citrus fruit juice 5.00 Fructose glucose liquid sugar 11.00 Citric acid 0.20 L-ascorbic acid · 0.02 Ganoderma processed product B 0.05 Spice 0.20 Pigment 0.10 Water 83.43 Total weight 100.00 (Example 9- 8) Carbonated beverage: 31 316979 200536486 [Table 13] Composition weight parts refined sugar 8.00 concentrated lemon juice 1.00 L-ascorbic acid 0.10 citric acid 0.09 sodium citrate 0.05 coloring material 0.05 spice 0.15 carbonated water 90.55 processed ganoderma lucidum B 0.01 total weight 100.00 (Examples 9-9) Lactobacillus lactic acid beverages [Table 14] Composition weight parts of fermented milk with 21% milk solid content 14.76 Fructose glucose liquid sugar 13.31 Pectin 0.50 Citric acid 0.08 Spice 0.15 Water 71.14 Ganoderma processed product B 0.06 All Weight 100.00 (Examples 9-10) Coffee σ non-beverage: 32 316979 200536486 [Table 15] Composition weight parts refined sugar Too ~ 'skim milk powder 5.00 caramel 0.20 coffee ° non-extract 2.00 flavor 0.10 polyglycerol fatty acid ester 0.05 Salt 0.05 Water 84.56 Ganoderma processed product B Total weight ^ ~ T〇〇T〇 ^ (Examples 9-11) Alcoholic beverages: [Table 16] 分 ι ^^ Τ〇〇8.40 2.40 0.20 ΤοοΤο? Sugar juice, Ganoderma lucidum processed product B, which has a content of 1 volume, pure water (industrial possibility) Action method, It can industrially manufacture antitumor substances or Ganoderma lucidum =: = objects or processed products of Ganoderma lucidum. Those who have made Ganoderma lucidum cancer carers have great effects, and they are very important for the housing industry.

I 33 3i6979 200536486 [Brief description of the drawings] Fig. 1 shows the measurement results of the extraction rate in Test Example 3. FIG. 2 shows the elution amount of / 5-glucan in Test Example 4. FIG.

34 316979

Claims (1)

200536486 10. Scope of patent application: 1. A method for manufacturing Ganoderma lucidum treatment, which is characterized by treating Ganoderma lucidum with water vapor of 1 50 to 2 1 0-C. 2. The method for manufacturing a Ganoderma lucidum treated product according to item 1 of the patent application scope, wherein ′ is treated with water vapor at 180 to 21 ° C. 3. The method for manufacturing a Ganoderma treatment product according to item 1 or 2 of the scope of patent application, wherein 'the water vapor is saturated water vapor. 4. The method for manufacturing a Ganoderma lucidum treatment product according to any one of claims 1 to 3 in the patent application scope, wherein the treatment is performed at an oxygen concentration of 0 to 1 # g / mL. 5 ·: The preparation of Ganoderma lucidum treated products in any one of items 1 to 4 of the patent scope, in which the water vapor treatment is performed for 1 to 30 minutes. Taken Zhizhi treatment, which contains 0.5% by weight or more of water-soluble 0-glucose 'I; Wai = Ganoderma lucidum treatment in the sixth item of the scope, using the patent application 8.-Zhonglingyi f 5 Produced by a manufacturing method. f, 〇 工 ° 〇Q 的 制造 方法 ’is characterized in that: Ganoderma lucidum obtained by treating Ganoderma with water vapor treated with o is cut or powdered, 2) is extracted with an aqueous solvent, or 3) is converted into 2). Aberdeen extract is further concentrated and refined or dried powder. 9 · A method for processing DD garments of Ganoderma lucidum, which is characterized by extracting Ganoderma lucidum with water from 150 to 21 0316979 35 200536486 ° C, or further concentrating the water extract. Refined or dried to powder. 10. The method for manufacturing a processed Ganoderma lucidum product according to item 9 of the patent application scope, wherein the water-soluble P-glucan is extracted into the processed Ganoderma lucidum product at a ratio of 0.5% by weight or more of the raw material Ganoderma lucidum. 11. A processed Ganoderma lucidum, which is manufactured by using the manufacturing method of the patent application No. 8, 9, or 10. 12. A food and drink product containing processed Ganoderma lucidum under the scope of patent application No. 6 or 7 or processed Ganoderma lucidum under the scope of patent application No. 11. 13. If the food or beverage of item 12 of the patent application scope is attached, it has a label with the main purpose of anti-tumor effect, immune activation effect or anti-cancer effect. 14. A pharmaceutical product, which contains processed Ganoderma lucidum in the scope of patent application No. 6 or 7, or processed Ganoderma lucidum in the scope of patent application No. 11. 15. If the medicine in the scope of patent application No. 14 is applied, the medicine is an antitumor agent. 36 316979