TW200530401A - Method of self cells transplantation for periodontosis - Google Patents

Abstract

This invention is related to provide a method of self cells transplantation for periodontosis which is mainly related to directly collect self healthy periodontal fibrous cells of patients and sequentially pass the cells through cultivation steps of dividing and cutting, centrifugal filtration, immersing in the cultivation solution, and sealed conservation. And add the freeze-dried decalcified bone (DFDBA), then transplant it back to the original suffering defect regions to achieve the purpose of repairing periodontal tissues.

Description

200530401 V. Description of the invention α) [Technical field to which the invention belongs] The present invention relates to a method for autologous cell transplantation of periodontal disease, in particular to a culture method for repairing damaged periodontal tissue using autologous cell transplantation. [Previous technology] According to medical explanation, periodontal disease is a disease that produces pathological changes in periodontal tissue (as shown in the first picture). According to the general periodontal tissue 5 mainly includes: Gingival tissue 1. Tooth Periodontal ligament'PDL 2, Aliveolar bone 3 and cementum; its cause is mainly caused by dental plaque (Dental PlaqUe), which causes erosion of periodontal tissue The current initial symptoms include redness, swelling, tooth brushing A, and bad breath. In addition to the above symptoms, the private peripheral ligament 2 under the gums is eroded to form a capsular bag. In severe cases, alveolar months 3 and tooth movement. Strict $ μ until bone loss and tooth loosening currently occur in the treatment of periodontal disease, including Secondary II to prevent recurrence and achieve periodontal tissue regeneration, commonly known as " washing teeth ", or using calculus on the tooth silver, The plaque and calculus scraping caused the periodontal group to heal the periodontal disease of the teeth on the surface of the tooth roots, which could not be the primary 'two, if severe periodontal surgery was suffered', the periodontal pocket was deepened :: and: need, dental sclerosis ^, 纟 U, Shi Yuanquan [Content of the invention] After the main method of cultivation of the present invention is to promote the restoration of teeth a, extraction b, and the front f; c, by Weaving and separation; d. Closed culture of the anterior fluid e. The number of mother cells is 200530401 V. Description of the invention (2) Binning: The alveolar bone that is damaged must be irregular ~ Now U system uses different periodontal Patients undergo membrane surgery and are equipped with reconstruction, such as bone graft and GTR membrane, using the method of root conditioning or periodontal tissue known on the root surface. Meal repairs have been previously, though, these methods can repair periodontal tissues and some bottlenecks need to be broken. There is still a reason in the treatment, how to provide a periodontal group is the research engine of the present invention. The method of living, that is, the main purpose is to provide a way to capture the patient's own body :::: the transfer of autologous tissue from the patient's body. Include: The periodontal tissue contained in the patient ’s own periodontal tissue is described as “f dimension mother cells; and then individually divided into centrifugation at high speed and centrifugation. The impurities and bacteria are filtered with periodontal grade in the periodontal group. Weaving and immersion is placed in a subculture containing culture, which is long and covered with the whole culture m; make the periodontal tissue fibers look at page 6 200530401 V. Description of the invention (3) f. Add cold beam dry decalcified bone (DFDBA) to the cell culture dish for co-cultivation. G. After periodontal fibroblasts are covered with freeze-dried decalcified bone, the periodontal tissue defect of the original patient will be implanted by periodontal surgery The components of the culture medium mainly include medium, growth hormone and antibiotics. The culture environment is about 37 ° C, 5% C02 humid conditions, so that cells can grow well. [Embodiment 】 Each, In order to make your reviewers fully understand the effectiveness of the content of the present invention, a specific embodiment is listed with the drawings, as follows:

The periodontal ligament or gingival tissue is harvested for cultivation. 3 Fine cheek second implantation method (please also include the following steps .. 1) Leash 2 or dental silver 1 tissue, containing fibroblasts (A)

By using a high-speed spinner, if using a speed blade, divide it into a centrifuge tube, and completely separate the tissue (C). Centrifuge it for 3 times for 0 minutes to achieve filtration. 200530401

Quality and bacteria. d. Immerse the periodontal leashes or gum tissues that have been divided into the culture orphan (D) containing the culture solution, and then cover them in order and store them in an incubator (the incubator) The environment is a humid environment of 37 I and 5% co2. In this example, the diameter of the petri dish is 6 cm), and the culture medium mainly contains culture genes (such as a_MEMALpHA_ Medium), which accounts for about 89%. , Growth hormone (such as: FB S Fetal

Bovine Serum) accounts for about 10% of the amount of bacteria and antibiotics (such as: = / 3'1 ^ 1 ^ 61 ^ 11/3 忖 61 ^ 〇 (^ 〇11, penicillin / streptomycin)) e cell number Adding 10 ^ 14 of dried periodontal diseased cells to culture dish f can promote the growth of the later, and then grow again, for example, after the freeze-dried days, calcium bones. By transplanting back

Subculture was performed the next day, so that the fibroblasts were covered with (E) the entire petri dish (with a cell density of approximately 5x104 with a diameter of 6 cm). Cloth culture containing periodontal ligaments or gingival fibroblasts m Co-cultivation with dry bone removal (DFDBA) (F) to completely cover the periodontal band or gingival fibroblasts

In this step, a gel-like mixture can be formed, and the appropriate amount of freeze-dried decalcified bone and fibroblasts can be collected to restore the defect (G) of the alveolar bone of the original patient, which is the phenomenon of tissue regeneration. In the present invention, the cells of the patient's body are used for culturing the defects of the body, and no rejection will occur.

200530401 V. Description of the invention (5) The present invention provides a method of autologous cell transplantation for periodontal disease, which is to repair periodontal tissue defects by autologous cells and improve the cure rate of periodontal disease, which has great industrial application value. In fact, it has fully met the requirements of novelty and progressive statutory invention patents, and submitted an application in accordance with the law. It is imperative that the Beihai Bureau approve this invention patent application to encourage inventions, and to the greatest extent. The above detailed description is a specific description of one of the feasible embodiments of the present invention, but this embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or change that does not depart from the technical spirit of the present invention shall include Within the scope of the patent in this case. i

200530401 Simple illustration of the drawing [Simplified illustration of the drawing] The first diagram is a schematic cross-sectional view of the human tooth structure. The second figure is a flow chart of the implementation steps of the present invention. [Representative symbols of main parts] 1 ...... periodontal tissue 2 ...... periodontal ligament 3 ...... alveolar bone 4 ...... cementum 5 .... .. Tooth (A) extracts fibroblasts (B) contained in periodontal ligament or gingival tissue, cuts them into an appropriate amount, and then separately distributes them into a centrifuge tube (C). High-speed centrifugation makes the impurity bacteria and fibers Complete isolation of mother cells (D) Immersion in a culture orphan containing culture medium (E) Subculture to make the number of fibroblasts grow to full (F) Add cold beam to dry out bone (G) Defective cementum

Page 10

Claims (1)

200530401 6. Scope of patent application, ^ β 1. The method of autologous cell transplantation of periodontal disease, including, extracting the patient's own periodontal tissue; Steps: 1. Dividing the aforementioned periodontal tissue, and then The knife is placed in the c-weave from the heart tube of ab, and the impurities are removed by centrifugation at high speed; 5 shells and bacteria and periodontal group d. The periodontal tissue dipped and immersed in the liquid through a closed Petri dish Cultivation: There are culture e, subculture, so that the number of dental mother cells grows and spreads throughout the entire petri dish; the fibers of the tissues and tissues f, freeze-dried drying Calcium bone (DFDBA) co-culture. " Add cold g to the culture dish and wait for periodontal fibroblasts to cover Lengkang's dry and dehydrated bones. * Surgical implantation will return to the periodontal tissue defect of the original patient. With periodontal 2, the periodontal implantation method as described in item 1 of the scope of the patent application, wherein the culture fluid components mainly include stilbene I autologous, cytoplasmic, and antibiotics. ° Soil, growth hormone 3, The dental implant method described in item 1 of the scope of patent application, wherein the time of immersion in the culture solution is about 15 ^, ,, and cell transfer 4, such as item i of the scope of patent application In the dental implantation method, the culture environment is about 37 t, and 5% 〇f L shift. The wet condition of L U2 5. As the first day of the patent application scope; m Implantation ♦ Installed in the tooth η ^ f stomach 'sick stomach $ field & transplantation method' Periodontal (weaving including periodontal palate and dental silver tissue 200530401 Page 12