TW200514985A - An immunochemiluminometric assay on PMMA chips for the measurement of serum HS-CRP - Google Patents
An immunochemiluminometric assay on PMMA chips for the measurement of serum HS-CRPInfo
- Publication number
- TW200514985A TW200514985A TW092128785A TW92128785A TW200514985A TW 200514985 A TW200514985 A TW 200514985A TW 092128785 A TW092128785 A TW 092128785A TW 92128785 A TW92128785 A TW 92128785A TW 200514985 A TW200514985 A TW 200514985A
- Authority
- TW
- Taiwan
- Prior art keywords
- assay
- crp
- chip
- immunochemiluminometric
- pmma
- Prior art date
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- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
This invention was aimed to develop an immunochemiluminometric assay on a polymethylmethacrylatepolymer (PMMA) chip for measuring C-reactive protein in ultra-sensitivity level. The PMMA chip was fabricated using the micro-electro-mechanical system (MEMS) technologies and activated by poly-L-lysine. An immunochemiluminometric assay was then performed on the top of chip. The capture monoclonal antibody was coated onto chips and the detection antibody labeled with acridinium ester. After the 2-step immunometric assay, alkaline hydroperoxide was added to detect the acridinium chemiluminescence signal. The linear range was within 0.01-50.00 mg/L. The analytical detection limit (mean of zero calibrator+2 SD) was 0.004 mg/L CRP. The intra-assay and inter-assay imprecision was < 7.3% and < 9.1%, respectively. Hemolysis, icterium and lipemia had no effect on the measurement. The analysis can be completed within 30 minutes. The assay correlated well with the Dade Behring N High Sensitivity-CRP assay (r=0.993, y=1.018x-0.806, Sy/x=1.186 mg/L, n=50). In conclusion, this assay is highly sensitive and precise and is feasible for the clinical use.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW92128785A TWI271519B (en) | 2003-10-16 | 2003-10-16 | An ultra-sensitivity immunochemiluminometric assay on PMMA chips for the measurement of serum hs-CRP |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW92128785A TWI271519B (en) | 2003-10-16 | 2003-10-16 | An ultra-sensitivity immunochemiluminometric assay on PMMA chips for the measurement of serum hs-CRP |
Publications (2)
Publication Number | Publication Date |
---|---|
TW200514985A true TW200514985A (en) | 2005-05-01 |
TWI271519B TWI271519B (en) | 2007-01-21 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW92128785A TWI271519B (en) | 2003-10-16 | 2003-10-16 | An ultra-sensitivity immunochemiluminometric assay on PMMA chips for the measurement of serum hs-CRP |
Country Status (1)
Country | Link |
---|---|
TW (1) | TWI271519B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102435741A (en) * | 2011-08-31 | 2012-05-02 | 内蒙古科慧生物科技有限责任公司 | Quantitative human high-sensitivity C-reactive protein (HS-CRP) determination kit and assay method thereof |
-
2003
- 2003-10-16 TW TW92128785A patent/TWI271519B/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102435741A (en) * | 2011-08-31 | 2012-05-02 | 内蒙古科慧生物科技有限责任公司 | Quantitative human high-sensitivity C-reactive protein (HS-CRP) determination kit and assay method thereof |
Also Published As
Publication number | Publication date |
---|---|
TWI271519B (en) | 2007-01-21 |
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Legal Events
Date | Code | Title | Description |
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MM4A | Annulment or lapse of patent due to non-payment of fees |