TW200514985A - An immunochemiluminometric assay on PMMA chips for the measurement of serum HS-CRP - Google Patents

An immunochemiluminometric assay on PMMA chips for the measurement of serum HS-CRP

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Publication number
TW200514985A
TW200514985A TW092128785A TW92128785A TW200514985A TW 200514985 A TW200514985 A TW 200514985A TW 092128785 A TW092128785 A TW 092128785A TW 92128785 A TW92128785 A TW 92128785A TW 200514985 A TW200514985 A TW 200514985A
Authority
TW
Taiwan
Prior art keywords
assay
crp
chip
immunochemiluminometric
pmma
Prior art date
Application number
TW092128785A
Other languages
Chinese (zh)
Other versions
TWI271519B (en
Inventor
Shu-Chu Shiesh
Tse-Chuan Chou
Liang-Ru Su
Hsin-I Lin
Original Assignee
Shu-Chu Shiesh
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Publication date
Application filed by Shu-Chu Shiesh filed Critical Shu-Chu Shiesh
Priority to TW92128785A priority Critical patent/TWI271519B/en
Publication of TW200514985A publication Critical patent/TW200514985A/en
Application granted granted Critical
Publication of TWI271519B publication Critical patent/TWI271519B/en

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  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

This invention was aimed to develop an immunochemiluminometric assay on a polymethylmethacrylatepolymer (PMMA) chip for measuring C-reactive protein in ultra-sensitivity level. The PMMA chip was fabricated using the micro-electro-mechanical system (MEMS) technologies and activated by poly-L-lysine. An immunochemiluminometric assay was then performed on the top of chip. The capture monoclonal antibody was coated onto chips and the detection antibody labeled with acridinium ester. After the 2-step immunometric assay, alkaline hydroperoxide was added to detect the acridinium chemiluminescence signal. The linear range was within 0.01-50.00 mg/L. The analytical detection limit (mean of zero calibrator+2 SD) was 0.004 mg/L CRP. The intra-assay and inter-assay imprecision was < 7.3% and < 9.1%, respectively. Hemolysis, icterium and lipemia had no effect on the measurement. The analysis can be completed within 30 minutes. The assay correlated well with the Dade Behring N High Sensitivity-CRP assay (r=0.993, y=1.018x-0.806, Sy/x=1.186 mg/L, n=50). In conclusion, this assay is highly sensitive and precise and is feasible for the clinical use.
TW92128785A 2003-10-16 2003-10-16 An ultra-sensitivity immunochemiluminometric assay on PMMA chips for the measurement of serum hs-CRP TWI271519B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW92128785A TWI271519B (en) 2003-10-16 2003-10-16 An ultra-sensitivity immunochemiluminometric assay on PMMA chips for the measurement of serum hs-CRP

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW92128785A TWI271519B (en) 2003-10-16 2003-10-16 An ultra-sensitivity immunochemiluminometric assay on PMMA chips for the measurement of serum hs-CRP

Publications (2)

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TW200514985A true TW200514985A (en) 2005-05-01
TWI271519B TWI271519B (en) 2007-01-21

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TW92128785A TWI271519B (en) 2003-10-16 2003-10-16 An ultra-sensitivity immunochemiluminometric assay on PMMA chips for the measurement of serum hs-CRP

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102435741A (en) * 2011-08-31 2012-05-02 内蒙古科慧生物科技有限责任公司 Quantitative human high-sensitivity C-reactive protein (HS-CRP) determination kit and assay method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102435741A (en) * 2011-08-31 2012-05-02 内蒙古科慧生物科技有限责任公司 Quantitative human high-sensitivity C-reactive protein (HS-CRP) determination kit and assay method thereof

Also Published As

Publication number Publication date
TWI271519B (en) 2007-01-21

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