CN204165984U - Cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper - Google Patents
Cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper Download PDFInfo
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- CN204165984U CN204165984U CN201420648352.3U CN201420648352U CN204165984U CN 204165984 U CN204165984 U CN 204165984U CN 201420648352 U CN201420648352 U CN 201420648352U CN 204165984 U CN204165984 U CN 204165984U
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Abstract
The utility model discloses a kind of cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper, comprise base plate, sample pad, fluorescent marker pad, cellulose nitrate and adsorptive pads; Described test strips is overlapped successively to be pasted onto on base plate formed by sample pad, fluorescent marker pad, cellulose nitrate, adsorptive pads.Described fluorescent marker pad contains marked by streptavidin fluorescin and biotin labeled cardiac muscle troponin I monoclonal antibody; Described nitrocellulose membrane has detection line and nature controlling line, detection line is by cardiac muscle troponin I monoclonal antibody, and it has different identification epi-positions from above-mentioned biotin labeled cardiac muscle troponin I monoclonal antibody.The utility model is (as: chemoluminescence method/Immune-enhancing effect turbidimetry/colloidal gold method) compared with the method for detection cardiac muscle troponin I common at present, not only substantially reduces detection time, also improves detection sensitivity simultaneously.
Description
Technical field
The utility model belongs to field of immunodetection, is specifically related to a kind of high sensitivity cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper.
Background technology
CTnI (cTnI) is a molecular weight is the myocardium protein of 22.5KD, it forms a cardiac Troponin complex together with TnT (TnT), TnC (TnC), the basic function that the Ca2+ oscillations jointly completing actin interphase interaction in cell transmits.In the clinical biochemistry indications of many diagnosing acute myocardial infarctions (AMI), be no matter the specificity to cardiac muscle or diagnostic sensitivity, cTnI is considered to determination mark best at present, has been widely used in clinical.
The common method of current detection cardiac muscle troponin I has:
1. radioimmunology (RIA)
Cummins in 1987 etc. establish the radiommunoassay that postprecipitation double antibody competition law detects cTnI the earliest.Its principle first anti-to test serum and a certain amount of rabbit cTnI antibody is mixed, hatched, then the cTnI of 125I mark is added, anti-cTnI antibody is competed with the cTnI in test serum, form antigen-antibody complex, this compound is combined to be formed with the monkey anti-rabbit IgG again added and precipitates, centrifugal removing supernatant, measures sedimentary activity, tries to achieve the concentration of cTnI according to typical curve.This method minimum detectability is 10 μ g/L, resist owing to using more, cross reaction can reach 25% when cTnI concentration is 20mg/L, and complicated operation, reaction time is long, meanwhile, owing to there is the danger of radiocontamination, its clinical practice is restricted, and therefore substantially safer by other, easy method replaces.
2. enzyme linked immunosorbent assay (ELISA)
At the mid-80, Cummins reports enzyme linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) and measures serum cTnI.This method by the how anti-mixing of serum to be checked and horseradish peroxidase mark, hatch, and then Incubating Solution to be added by the microwell plate of cTnI bag quilt.Resisting because the cTnI in the cTnI of bag quilt and serum to be checked competes more, make HRP be indirectly fixed on microwell plate, by measuring the catalytic activity of HRP in microwell plate, calculating the concentration of cTnI with standard curve control.This method minimum detectability is 4 μ g/L, and the reaction time reaches 5-6h.Resist owing to employing in mensuration, cross reaction cannot be eliminated, and has some limitations more.Larve in 1993 etc. improve further, from the monoclonal antibody of 28 kinds of purifying, filter out the very strong antibody of a species specificity for immunoassay, replace alkaline phosphatase with HRP, the sensitivity of mensuration is significantly improved, its detect and track is 0.1-0.2 μ g/L, completes test in 30min.ELISA method is the method for the mensuration cTnI that studies in China is maximum, the method sensitivity is moderate, use relatively easy, instrument price is also not bery high, but the method also also exists many deficiencies, as determination period is partially long, sensitivity is relatively low, need the operating personnel etc. being equipped with special instrument and specialty.
3. chemoluminescence method
Utilizing chemiluminescence determination cTnI more is at present the Access analytic system adopting Beckman coulter company.This method application double antibody one step sandwich enzyme immunoassay method, using ALP as label, with chemiluminescence agent (dichloroethane derivant) for substrate, be solid phase carrier with the magnetic particle of monoclonal anti cTnI IgG antibody bag quilt, after hatching, wash, being separated, measure the luminous intensity of the luminous substrate added, calculate cTnI concentration by multiple spot calibration curve.The chemiluminescence system of the mensuration cTnI that an other class application is comparatively general is with the Acs-180 of BAYER company of U.S. series automatic chemiluminescence immunoassay system representatively, it stings smack one's lips vinegar (AE) as the direct labelled antigen of label or antibody using Small molecular luminescent substance, after immune response, through fully washing, finally add alkaline auxiliary reagent, detect its luminous intensity, thus quantitative to cTnI.Chemoluminescence method is safe, sensitive, highly versatile, it is a kind of method of global each major company active development, particularly after chemiluminescent substance synthesis as overdelicate in dichloroethane classes such as AMPPD, its investigation and application is extensive especially, but ELISA method relatively, the method instrument cost is relatively high.
4. fluorometry
Dade Stratus II is a kind of robotization two-site tluorimetric enzyme immunoassay detection method, it is the cTnI detection system that first hand is ratified by U.S. FDA, so the cTnI analytic system developed afterwards more be using it as assay standard, on the basis of this system, Dade Behring company is proposed again the product Stratus CS Delta cTnI of a new generation.France bioMerieux recently develop one measures enzyme connection system of fluorescence analysis (VIDAS cTnI) of cTnI, have in cTnI detects easy and simple to handle, automaticity is high, mensuration is quick, the result feature such as accurately and reliably.VIDAS cTnI detection kit adopts a step immunofluorescence technique, to connect the anti-cTnI monoclonal antibody bag of ALP by solid phase carrier (SPR), be inhaled in SPR pipe at blood serum sample and be combined with the cTnI antibody of bag quilt, and be fixed on SPR inwall and formed sandwich, unconjugated cTnI is removed by washing.Substrate 4-methyl umbelliferone phosphate (4-MUP) generates fluorescence-causing substance 4-methyl umbelliferone under ALP catalysis, in 450nm fluorescence intensity, more automatically calculates serum cTnI concentration with typical curve.Its sensing range be 0.1-50 μ g/L, CV < 5%, AMI critical value complete >=0.8 μ g/L, instrument correction in every 2 weeks 1 time, cross reaction only has 1.6% when 60 μ g/L, does not almost have cross reaction with sTnT, sTnI and TnC etc.
5. gold-marking immunity method
Current gold-marking immunity method is mainly applied in the golden immunochromatographic method (GCIA) combined with immunochromatography technique, and this method sample consumption is few, fast easy, is suitable for the other detection of bed of AMI.External research is in this respect relatively many, as Princeton Biomedicines, Inc., Spectral Diagnostics company, Cortez company, Bioseed Diagnostics company, German Lai Bang company, An Li bio tech ltd of Finland etc. are all proposed the cTnI test card utilizing gold-marking immunity ratio juris to prepare, its ultimate principle all: detect free cTnI and compound cTnI with the single combination of two monoclonal anti-cTnI antibody.When sample drips in sucking, first anti-cTnI antibody/collaurum with cTnI in conjunction with formation antibody/antigen combination.Second immobilized anti-this combination of cTnI antibody capture, produces a peach color belt, occurs just not having colour band in reacting ring relative to without combination.
Because the antibody lacking common demarcation standard and/or use has cross reaction from different cTnI forms, so more than 10 times can be reached by cTnI concentration (normal reference value from < 0.2 μ g/L to the < 3.1 μ g/L not etc.) difference measured by different detection system.Because of the epitope district that people cTnI amino terminal and carboxyl terminal are the strongest, but this region is comparatively responsive to oxidation, reduction and phosphorylation, and be also easily hydrolyzed by endogenous proteolytic enzymes, different states possesses different immunocompetences.Due to the protection of TnC, most stabilized zone between 30 ~ 110 amino acid residues, only with for cTnI amino terminal or/and the antibody of carboxyl terminal will reduce susceptibility.Therefore advise that the antibody that cTnI sandwich immunoassay is selected preferentially should identify stable region, susceptibility and the repeatability of cTnI detection could be improved like this, contribute to realizing cTnI standardized testing.
Biotin-avidin system (biotinavidin system, BAS) is a kind of new bio reaction amplification system.Along with the appearance of various biotin derivative, BAS is widely used in each field of medical science very soon.This system is applied to SABC, enzyme linked immunological, fluorescence immunoassay, in the detection techniques such as radio-immunity, the susceptibility of above technical method, specificity and stability can be improved significantly, make method easier, contribute to clinical quick diagnosis, and be beneficial to and carry out extensive epidemiology survey, become the immunoreactive powerful of research.Because BAS detection system economy is quick, pollute without radiating matter again, do not need complex instrument, fully show the great potential of this system and the possibility of application.
Biotin-avidin system Cleaning Principle: BAS is on the basis of conventional ELISA principle, in conjunction with the height amplification between biotin and Avidin, and a kind of detection system set up.Avidin is a kind of alkaline glycoprotein extracted in ovalbumin, and molecular weight is 68kDa, is made up of 4 subunits, have very high affinity (binding constant is up to 1015M-1) to biology.Biotin is very easily combined with covalent bond with protein (as antibody etc.).Like this, the Avidin molecule combining enzyme and the biotin molecule being combined with specific antibody produce and react, both served multistage amplification, the colour generation due to the catalytic action of enzyme when running into corresponding substrate, reaches the object detecting unknown antigen (or antibody) molecule again.Streptavidin is and the affine a kind of protein have similar biological properties, in Streptavidin molecule the same as Avidin, every bar peptide chain can in conjunction with a biotin, because nearly all material for marking all can combine with Avidin or streptavidin.Utilize biotin-avidin system to develop cardiac muscle troponin I detection examination fast diagnosis reagent and there is no report.
Utility model content
The purpose of this utility model, be to provide a kind of cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper, it provides high sensitivity cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper based on double antibody sandwich method, fluorescence immunoassay lateral chromatography and biotin-Streptavidin amplification system.When using the utility model, improve detection sensitivity and detect stability, reducing non-specific binding, be not more than 10 minutes detection time, substantially increase diagnosis efficiency.
The solution that the utility model solves its technical matters is: cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper, it comprises base plate, described base plate is provided with sample pad successively, fluorescent marker pad, nitrocellulose filter and adsorptive pads, described adsorptive pads and fluorescent marker pad are overlapping to be respectively pressed in behind the two ends of nitrocellulose filter in the formation detection zone, surface of nitrocellulose filter, described sample pad is overlapping to be pressed on fluorescent marker pad, described fluorescent marker pad is fixed with the fluorescin of biotin labeled cardiac muscle troponin I monoclonal antibody and marked by streptavidin, nitrocellulose filter in described detection zone is fixed with the nature controlling line of detection line and the sheep anti-mouse igg polyclonal antibody formation identifying that the monoclonal antibody of cardiac muscle troponin I another one epi-position is formed.
As the further improvement of technique scheme, described fluorescent marker pad comprises the first stacked fluorescent marker pad and the second fluorescent marker pad, one end pad of described first fluorescent marker pad in the below of sample pad, described second overlapping one end being pressed in nitrocellulose filter of fluorescent marker pad.
As the further improvement of technique scheme, in described first fluorescent marker pad, the one end of inserting below sample pad is provided with positioning strip, and the lower surface of described sample pad is provided with the locating slot that can hold positioning strip.
As the further improvement of technique scheme, also comprise and getting stuck, in described base plate, sample pad, fluorescent marker pad, nitrocellulose filter and adsorptive pads are all placed in and get stuck, the position described shell face of getting stuck corresponding to nitrocellulose membrane is provided with view window, and position shell face of getting stuck corresponding to sample pad is provided with well.
As the further improvement of technique scheme, described view window is slot.
As the further improvement of technique scheme, described fluorescin can be the one in green fluorescent protein, phycobniliprotein etc.
As the further improvement of technique scheme, described sample pad is glass fibre component dry after surfactant damping fluid immersion treatment.
As the further improvement of technique scheme, described base plate is polystyrene component or tygon component.
As the further improvement of technique scheme, described in get stuck and comprise plastics upper casing and plastics lower casing, described plastics upper casing fastens to be formed after on plastics lower casing and gets stuck.
Compared with prior art, tool has the following advantages the utility model:
(1) the utility model is using fluorescin as label, and this label has good stability, and is conducive to improving detecting stability.
(2) the utility model is by utilizing " Streptavidin-biotin amplification system ", improves detection sensitivity, reduces non-specific binding, is conducive to improving kit performance.
(3) detection time utilizing the utility model to carry out Procalcitonin is not more than 10 minutes, and the detection range of linearity is 0.10ng/ml ~ 100.00ng/ml, drastically increases detection efficiency.
(4) the utility model carries out interpretation by fluorescence immunity analyzer to result, can realize robotization, reduces the impact of subjective factor, provides convenient, quick, reliable diagnostic result.
(5) novel the getting stuck of this use is provided with the well that sample enters and the view window supplying observed result, according to analytical instrument result of determination, accurately and reliably.
(6) the utility model is easy to make, and volume is little, be easy to carry.
(7) the utility model testing cost is lower.
(8) the utility model can be mass, and is applicable to clinical quick diagnosis and on-the-spot quick diagnosis; Be easy to preserve, be conducive to grass-roots unit and promote.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the utility model embodiment, below the accompanying drawing used required in describing embodiment is briefly described.Obviously, described accompanying drawing is a part of embodiment of the present utility model, instead of whole embodiment, and those skilled in the art, under the prerequisite not paying creative work, can also obtain other design proposals and accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation of the utility model embodiment one;
Fig. 2 is the structural representation of the utility model embodiment two;
Fig. 3 is the structural representation of the utility model embodiment three;
Fig. 4 is the structural representation of the utility model embodiment four.
Embodiment
Be clearly and completely described below with reference to embodiment and the accompanying drawing technique effect to design of the present utility model, concrete structure and generation, to understand the purpose of this utility model, characteristic sum effect fully.Obviously; described embodiment is a part of embodiment of the present utility model, instead of whole embodiment, based on embodiment of the present utility model; other embodiments that those skilled in the art obtains under the prerequisite not paying creative work, all belong to the scope of the utility model protection.In addition, all connection/annexations mentioned in literary composition, not singly refer to that component directly connects, and refer to and according to concrete performance, can connect auxiliary by adding or reducing, and form more excellent draw bail.
With reference to Fig. 1, cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper, it comprises base plate 5, described base plate 5 is provided with sample pad 1 successively, fluorescent marker pad 2, nitrocellulose filter 3 and adsorptive pads 4, described adsorptive pads 4 and fluorescent marker pad 2 are overlapping to be respectively pressed in behind the two ends of nitrocellulose filter 3 in the formation detection zone, surface of nitrocellulose filter 3, fluorescent marker pad 2 is glass fibre membrane, described sample pad 1 is overlapping to be pressed on fluorescent marker pad 2, the fluorescin described fluorescent marker pad 2 being fixed with biotin labeled cardiac muscle troponin I monoclonal antibody (concentration 0.3 ~ 1.5mg/mL) and marked by streptavidin (or Avidin) (uses excitation wavelength 530nm, wavelength of transmitted light 570nm, concentration 0.1 ~ 1.0mg/mL), nitrocellulose filter 3 in described detection zone is fixed with the nature controlling line C (concentration 0.2 ~ 2.0mg/mL) of detection line T (concentration 0.5 ~ 3mg/mL) and the sheep anti-mouse igg polyclonal antibody formation identifying that the monoclonal antibody of cardiac muscle troponin I another one epi-position is formed, nature controlling line C is used for the validity of test strip.
The fluorescin that biotin labeled cardiac muscle troponin I monoclonal antibody and Streptavidin (or Avidin) mark is fixed on fluorescent marker pad 2, the monoclonal antibody and sheep anti-mouse igg polyclonal antibody that have identification cardiac muscle troponin I another one epi-position are fixed on nitrocellulose filter as detection line T and nature controlling line C.When testing sample is added to after in sample pad 1, moved forward by chromatography effect, on sample Myocardial Troponin I and fluorescent marker pad 2, the cardiac muscle troponin I antibody (Mab-CTNI*Fluoro) of combined with fluorescent label (fluorescin) reacts and forms compound CTNI-Mab-CTNI*Fluoro, compound is reacted when continuing to be advanced past CTNI antibody (detection line) nitrocellulose membrane wrapping quilt under chromatography effect, CTNI antibody capture formation compound (Mab-CTNI-CTNI-Mab-CTNI*Fluoro) (detection line) that reaction compound is coated, the reaction signal of detection line is read by fluorescence immunity analyzer, under the effect of excitation source, fluorescent material launches the fluorescence signal of specific wavelength, fluorescence immunity analyzer captures fluorescence signal, the typical curve transformed by signal and set is converted into quantitative value automatically, calculate the concentration of center of a sample's flesh Troponin I, obtain cardiac muscle troponin I testing result.
As the further improvement of technique scheme, with reference to Fig. 3, described fluorescent marker pad 2 comprises the first stacked fluorescent marker pad 20 and the second fluorescent marker pad 21, one end pad of described first fluorescent marker pad 20 in the below of sample pad 1, described second overlapping one end being pressed in nitrocellulose filter 3 of fluorescent marker pad 21.The layering of fluorescent marker pad 2 is arranged, and is convenient to fluorescent marker pad 2 to be arranged between sample pad 1 and nitrocellulose filter 3.
As the further improvement of technique scheme, be provided with positioning strip 22 with reference to one end of inserting below sample pad in the first fluorescent marker pad 20 described in Fig. 3, the lower surface of described sample pad 1 is provided with the locating slot that can hold positioning strip 22.The installation be convenient between fluorescent marker pad 2 and sample pad 1 by positioning strip 22 is fixed.
As the further improvement of technique scheme, with reference to Fig. 2 and Fig. 4, also comprise and getting stuck, described base plate 5, sample pad 1, fluorescent marker pad 2, nitrocellulose filter 3 and adsorptive pads 4 are all placed in and get stuck in 6, the position described 6 shell faces of getting stuck corresponding to nitrocellulose membrane 3 is provided with view window 8, and the position 6 shell faces of getting stuck corresponding to sample pad 1 is provided with well 7.
As the further improvement of technique scheme, described view window 8 is slot.
As the further improvement of technique scheme, described fluorescin can be the one in green fluorescent protein, phycobniliprotein etc.
As the further improvement of technique scheme, described sample pad 1 is glass fibre component dry after surfactant damping fluid immersion treatment.Sample pad is made up of glass fibre, through surfactant damping fluid immersion treatment, uses after dry.The cutting width of described sample pad 1 is 0.3 ~ 0.5cm
As the further improvement of technique scheme, described base plate 5 is polystyrene component or tygon component.
As the further improvement of technique scheme, described in get stuck and 6 comprise plastics upper casing 60 and plastics lower casing 61, described plastics upper casing 61 fastens to be formed after on plastics lower casing 61 and gets stuck 6.
More than that better embodiment of the present utility model is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modifications or replacement under the prerequisite without prejudice to the utility model spirit, and these equivalent modification or replacement are all included in the application's claim limited range.
Claims (9)
1. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper, it is characterized in that: it comprises base plate, described base plate is provided with sample pad successively, fluorescent marker pad, nitrocellulose filter and adsorptive pads, described adsorptive pads and fluorescent marker pad are overlapping to be respectively pressed in behind the two ends of nitrocellulose filter in the formation detection zone, surface of nitrocellulose filter, described sample pad is overlapping to be pressed on fluorescent marker pad, described fluorescent marker pad is fixed with the fluorescin of biotin labeled cardiac muscle troponin I monoclonal antibody and marked by streptavidin, nitrocellulose filter in described detection zone is fixed with the nature controlling line of detection line and the sheep anti-mouse igg polyclonal antibody formation identifying that the monoclonal antibody of cardiac muscle troponin I another one epi-position is formed.
2. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to claim 1, it is characterized in that: described fluorescent marker pad comprises the first stacked fluorescent marker pad and the second fluorescent marker pad, one end pad of described first fluorescent marker pad in the below of sample pad, described second overlapping one end being pressed in nitrocellulose filter of fluorescent marker pad.
3. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to claim 2, it is characterized in that: in described first fluorescent marker pad, the one end of inserting below sample pad is provided with positioning strip, and the lower surface of described sample pad is provided with the locating slot that can hold positioning strip.
4. the cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to any one of claims 1 to 3, it is characterized in that: also comprise and getting stuck, in described base plate, sample pad, fluorescent marker pad, nitrocellulose filter and adsorptive pads are all placed in and get stuck, the position described shell face of getting stuck corresponding to nitrocellulose membrane is provided with view window, and position shell face of getting stuck corresponding to sample pad is provided with well.
5. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described view window is slot.
6. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described fluorescin can be the one in green fluorescent protein, phycobniliprotein etc.
7. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described sample pad is glass fibre component dry after surfactant damping fluid immersion treatment.
8. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described base plate is polystyrene component or tygon component.
9. cardiac muscle troponin I immunochromatographiassay assay quantitative detection test paper according to claim 4, is characterized in that: described in get stuck and comprise plastics upper casing and plastics lower casing, described plastics upper casing fastens to be formed after on plastics lower casing and gets stuck.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105527445A (en) * | 2015-12-30 | 2016-04-27 | 天津诺星生物医药科技有限公司 | Acute myocardial infarction detection system |
| CN105759050A (en) * | 2016-01-29 | 2016-07-13 | 苏州联辰生物技术有限公司 | Immunofluorescence kit for quantitatively detecting content of troponin I and preparation method |
| CN106290832A (en) * | 2016-08-12 | 2017-01-04 | 上海铭源数康生物芯片有限公司 | A kind of immunity lateral chromatography quantitative detecting reagent and preparation method thereof, detection method |
| CN107870238A (en) * | 2016-09-28 | 2018-04-03 | 上海仪电分析仪器有限公司 | Troponin I in a kind of quantitative measurment human serum（cTnI）Method |
| CN109709339A (en) * | 2018-12-28 | 2019-05-03 | 河北省科学院生物研究所 | Detect colloidal gold immuno-chromatography test paper strip and the application of ox or ovine skeletal muscle Troponin I |
| CN110272502A (en) * | 2019-07-12 | 2019-09-24 | 深圳市亚辉龙生物科技股份有限公司 | The hybridoma and preparation method, monoclonal antibody and application of immunogene, the anti-cardiac muscle troponin I monoclonal antibody of secretion |
| CN110702901A (en) * | 2019-10-10 | 2020-01-17 | 南京欧凯生物科技有限公司 | Fluorescence immunochromatography test paper for detecting cardiac troponin I |
| CN115389760A (en) * | 2022-10-27 | 2022-11-25 | 艾康生物技术(杭州)有限公司 | Detection reagent for immunoassay test strip |
| CN115963274A (en) * | 2022-09-30 | 2023-04-14 | 中国科学院长春应用化学研究所 | Detection reagent and method for cardiac troponin I |
| CN115980343A (en) * | 2023-03-21 | 2023-04-18 | 北京大学 | Chemiluminescence side-stream immunodetection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105527445B (en) * | 2015-12-30 | 2018-04-17 | 天津诺星生物医药科技有限公司 | A kind of acute myocardial infarction detecting system |
| CN105527445A (en) * | 2015-12-30 | 2016-04-27 | 天津诺星生物医药科技有限公司 | Acute myocardial infarction detection system |
| CN105759050A (en) * | 2016-01-29 | 2016-07-13 | 苏州联辰生物技术有限公司 | Immunofluorescence kit for quantitatively detecting content of troponin I and preparation method |
| CN106290832A (en) * | 2016-08-12 | 2017-01-04 | 上海铭源数康生物芯片有限公司 | A kind of immunity lateral chromatography quantitative detecting reagent and preparation method thereof, detection method |
| CN107870238A (en) * | 2016-09-28 | 2018-04-03 | 上海仪电分析仪器有限公司 | Troponin I in a kind of quantitative measurment human serum（cTnI）Method |
| CN107870238B (en) * | 2016-09-28 | 2023-12-26 | 上海仪电分析仪器有限公司 | Method for quantitatively measuring troponin I (cTnI) in human serum |
| CN109709339B (en) * | 2018-12-28 | 2022-03-15 | 河北省科学院生物研究所 | Colloidal gold immunochromatographic test strip for detecting skeletal muscle troponin I of cattle or sheep and application thereof |
| CN109709339A (en) * | 2018-12-28 | 2019-05-03 | 河北省科学院生物研究所 | Detect colloidal gold immuno-chromatography test paper strip and the application of ox or ovine skeletal muscle Troponin I |
| CN110272502A (en) * | 2019-07-12 | 2019-09-24 | 深圳市亚辉龙生物科技股份有限公司 | The hybridoma and preparation method, monoclonal antibody and application of immunogene, the anti-cardiac muscle troponin I monoclonal antibody of secretion |
| CN110702901A (en) * | 2019-10-10 | 2020-01-17 | 南京欧凯生物科技有限公司 | Fluorescence immunochromatography test paper for detecting cardiac troponin I |
| CN115963274A (en) * | 2022-09-30 | 2023-04-14 | 中国科学院长春应用化学研究所 | Detection reagent and method for cardiac troponin I |
| CN115389760A (en) * | 2022-10-27 | 2022-11-25 | 艾康生物技术(杭州)有限公司 | Detection reagent for immunoassay test strip |
| CN115980343A (en) * | 2023-03-21 | 2023-04-18 | 北京大学 | Chemiluminescence side-stream immunodetection method |
| CN115980343B (en) * | 2023-03-21 | 2023-07-07 | 北京大学 | Chemiluminescent lateral flow immunoassay method |
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