TW200500463A - Process for improving efficiency of DNA amplification reaction - Google Patents

Process for improving efficiency of DNA amplification reaction

Info

Publication number
TW200500463A
TW200500463A TW092116966A TW92116966A TW200500463A TW 200500463 A TW200500463 A TW 200500463A TW 092116966 A TW092116966 A TW 092116966A TW 92116966 A TW92116966 A TW 92116966A TW 200500463 A TW200500463 A TW 200500463A
Authority
TW
Taiwan
Prior art keywords
amplification reaction
dna amplification
improving
dna
oligonucleotide
Prior art date
Application number
TW092116966A
Other languages
Chinese (zh)
Inventor
Takeshi Koizumi
Yoko Hamano
Satoshi Yamamoto
Original Assignee
Nichirei Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2002003912A external-priority patent/JP2003199568A/en
Application filed by Nichirei Kk filed Critical Nichirei Kk
Publication of TW200500463A publication Critical patent/TW200500463A/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention has an object of providing a process for improving the efficiency of a DNA amplification reaction, and a process for improving the hybridization specificity of an oligonucleotide to a DNA. The present invention provides a process for improving the efficiency of a DNA amplification reaction, wherein a primer in which a compound such as LC-Red 705 or an oligonucleotide with a GC content of at least 25% and with at least four bases is added to the 5' terminus is used as the primer; as well as a process for improving the hybridization specificity of an oligonucleotide to a DNA sample, wherein an oligonucleotide in which a compound such as LC-Red 705 is conjugated to the 5' terminus is used for hybridizing to a DNA.
TW092116966A 2002-01-10 2003-06-23 Process for improving efficiency of DNA amplification reaction TW200500463A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2002003912A JP2003199568A (en) 2002-01-10 2002-01-10 Method for improving efficiency of dna amplification reaction

Publications (1)

Publication Number Publication Date
TW200500463A true TW200500463A (en) 2005-01-01

Family

ID=57797943

Family Applications (1)

Application Number Title Priority Date Filing Date
TW092116966A TW200500463A (en) 2002-01-10 2003-06-23 Process for improving efficiency of DNA amplification reaction

Country Status (1)

Country Link
TW (1) TW200500463A (en)

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