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Priority claimed from JP2002003912Aexternal-prioritypatent/JP2003199568A/en
Application filed by Nichirei KkfiledCriticalNichirei Kk
Publication of TW200500463ApublicationCriticalpatent/TW200500463A/en
Measuring Or Testing Involving Enzymes Or Micro-Organisms
(AREA)
Abstract
The present invention has an object of providing a process for improving the efficiency of a DNA amplification reaction, and a process for improving the hybridization specificity of an oligonucleotide to a DNA. The present invention provides a process for improving the efficiency of a DNA amplification reaction, wherein a primer in which a compound such as LC-Red 705 or an oligonucleotide with a GC content of at least 25% and with at least four bases is added to the 5' terminus is used as the primer; as well as a process for improving the hybridization specificity of an oligonucleotide to a DNA sample, wherein an oligonucleotide in which a compound such as LC-Red 705 is conjugated to the 5' terminus is used for hybridizing to a DNA.
TW092116966A2002-01-102003-06-23Process for improving efficiency of DNA amplification reaction
TW200500463A
(en)