200425837 玖、發萌說明, . 【發明所屬之技術領域】 本發明係有關於一種提升乳品中機能性物質 含量之方法,尤指一種在反芻動物飼料中添加含 高量 18 石炭 2 稀酸(C18:2,Linoleic acid)之油 0S , 使反芻動物所生產之乳品中含有高量共輒亞麻油 酸之機能性物質者。 【先前技術】 乳品為普遍且營養價值高之食物,若能促使 反芻動物在自然條件下生產含高量機能性物質之 生乳,不但可提升人類所霈之營養,且具有增強 保健免疫力,甚至豁療之效果,古云:『殺食同 源』。因此本發明乃針對此一機能性乳品進行研 究開發,將對人體健康具有幫助之營養物質,藉 由反芻動物做為生物工廠,轉置於所生產的生乳 中 〇 【内容】 本發明係一種提升乳品中機能性物質含量的 方法,其主要係於反芻動物飼料中添加1 · 5至8重 量%含高量18碳 2稀酸(C18:2,Linoleic acid)之油 脂(其油脂為紅花狩油、玉米油、棉籽油、黃豆油 、芝麻油、劈花狩油、花生油),其經均勻的混合 後餓飼反芻動物,並於餓飼後第1 4天起收集反芻 動物之乳,即為含有高量共輒亞麻油酸之機能性 乳品,由於該機能性乳品含有對人體健康具有幫 助之營養物質,不但可提供人類所需之營養且具 ^ -—-—^ 200425837 玖'、廣明說明: 有保健及增強免疫力,甚至翳療效果,為一前所 未見,且具有產業上利用價值的發明。 【實施方式】 本發明化學結構:G 02 (共軛亞麻油酸 ,Conjugated Linoleic Acid,CLA),共輒亞麻油 酸則為亞麻油酸之幾何異構物,由八種Μ上包含 共輕雙鍵糸統(Conjugated double bond system) 之異構物所組成,以cis-Q.tr'ans-ll共輕亞麻油 酸為主* 因其能併入生物膜之鱗脂質中,被認為 是具有生物活性之共軛亞麻油酸。 本發明提升乳品中機能性物質含虽的方法, 亦係一種提升反芻動物乳中共軛亞麻油酸含量之 方法,其主要係於反芻動物飼料中添加1.5-8重量 % 含高量 1 8 碳 2 mm ( C 1 8 : 2 , L i η ο 1 e I c a c i d )之 '?虫 脂,其經均匀混和後餵飼反芻動物,於餓飼後1 4 天起所收集之乳品,即為含髙量共軛亞麻油酸之 機能性乳品。 前述可提升乳中共軛亞麻油酸之含高童18碳 2 mm (C18: 2, Linoleic a c i d )之卞由 0 旨 4系為含有 3 0 % Μ上之1 8碳卩烯酸之油脂,該油脂為紅花舒油 、玉米油、棉籽油、黃豆油、芝麻油、藥花籽油 花生油。 前述反芻動物為牛、羊或其他反芻動物,乳 品為牛乳、、羊乳或其他反芻動物乳。 本發明之生理功能為: 1 .抗癌機制 200425837 Β ο υ g n u x e t al · (1999)針對360名乳癌患者 進行臨床治療,發現C L A對乳癌腫瘤細胞有減 媛擴大的效果,C L A與乳癌圼顯著的負相關。 Schultz e t al,(1992)發現CLA對人類黑色素腫 瘤,結腸直腸癌綑胞及乳癌细包有毒殺作用, 可明顯減少癌症的病狀以及抑制癌细胞的生長。 2 . C L A對心血管疾病的預防200425837 Explanation of germination and germination, [Technical field to which the invention belongs] The present invention relates to a method for improving the content of functional substances in dairy products, especially a method for adding a high content of 18 charcoal 2 dilute acid (C18 : 2, Linoleic acid) oil 0S, so that dairy products produced by ruminants contain high amounts of functional substances of linoleic acid. [Previous technology] Dairy is a universal and high nutritional value food. If it can promote ruminant animals to produce raw milk with a high amount of functional substances under natural conditions, it will not only enhance human nutrition, but also enhance health immunity, and even The effect of extinction therapy, Gu Yun: "kill the same food". Therefore, the present invention is directed to research and development of this functional dairy product. Nutrients that are helpful to human health are transferred to the raw milk produced by ruminants as a biological factory. [Content] The present invention is an improvement The method for the content of functional substances in dairy products is mainly based on the addition of 1 · 5 to 8% by weight of fats with high content of 18 carbon 2 dilute acid (C18: 2, Linoleic acid) in ruminant feed. , Corn oil, cottonseed oil, soybean oil, sesame oil, cut flower vet oil, peanut oil), which are fed hungry to ruminants after homogeneous mixing, and the milk of ruminants is collected from the 14th day after starvation. Functional dairy products with high content of linoleic acid. Because the functional dairy products contain nutrients that are helpful to human health, they not only provide the nutrients that humans need, but also have ^ -—-— ^ 200425837 玖 ', Guangming description: An invention with health care and immunity enhancement, and even palliative effects, which has never been seen before, and has industrial utility value. [Embodiment] The chemical structure of the present invention: G 02 (Conjugated Linoleic Acid, CLA). Conjugated linoleic acid is a geometrical isomer of linoleic acid. Conjugated double bond system (Conjugated double bond system) is composed of isomers, mainly based on cis-Q.tr'ans-ll co-linolenic acid * Because it can be incorporated into biofilm scale lipids, it is considered to have Bioactive Conjugated Linoleic Acid. The method for improving the content of functional substances in dairy products according to the present invention is also a method for increasing the content of conjugated linoleic acid in ruminant milk, which is mainly based on the addition of 1.5-8% by weight in ruminant feed with a high content of 1 8 carbon 2 mm (C 1 8 : 2, L i η ο 1 e I cacid) of the insect fat, which is uniformly mixed and fed to ruminants, and the milk collected from 14 days after starvation is the content containing 髙A functional dairy product with conjugated linoleic acid. The above-mentioned conjugated linoleic acid-containing high-consumer 18-carbon 2 mm (C18: 2, Linoleic acid) can improve the fat from 0 to 4 is a fat containing 18-carbapinenoic acid on 30% Μ, the Oils and fats are safflower oil, corn oil, cottonseed oil, soybean oil, sesame oil, and flower seed oil and peanut oil. The aforementioned ruminant is cow, sheep or other ruminant, and the dairy product is cow's milk, goat's milk or other ruminant's milk. The physiological functions of the present invention are: 1. Anti-cancer mechanism 200425837 Β υ gnuxet al · (1999) Clinical treatment of 360 breast cancer patients, found that CLA has a reducing effect on breast cancer tumor cells, CLA and breast cancer are significantly different. Negative correlation. Schultz et al (1992) found that CLA has a toxic effect on human melanoma, colorectal cancer cells, and breast cancer cells, which can significantly reduce the symptoms of cancer and inhibit the growth of cancer cells. 2. C L A for the prevention of cardiovascular disease
Lee e t al· (1994)指出,共輒亞麻油酸具 有抗動物粥樣硬化Cantiatherosclerosis)之效 果。C L A可有效降低倉鼠血液中總膽固醇濃度, 包括低密度脂蛋白(low density lipoprotein ,LDL)膽固醇及極低密度脂蛋白(very low density 1 ipoprotein, VLDL)膽固酉莩之含:fi: , ffn 高密度 0首蛋白(high density lipoprotein, HDL) 則無影響,顯示C L A有助於預防動脈硬化的發生 (N i c ο 1 〇 s; i At a 丨-,1997) ° 3. CLA對免疫糸統之影響Lee et al. (1994) pointed out that linolenic acid has an anti-canceratherosclerosis effect. CLA can effectively reduce total cholesterol concentration in hamster blood, including low density lipoprotein (LDL) cholesterol and very low density 1 ipoprotein (VLDL). Cholesterol content: fi:, ffn High density lipoprotein (HDL) has no effect, showing that CLA helps prevent the occurrence of arteriosclerosis (N ic ο 1 〇s; i At a 丨-, 1997) ° 3. CLA on immune system Influence
Pa「iza(1999)指出,共輒亞麻油酸具有抑 制脂質之過氧化、抑制前列腺素之產量,因而 有免疫促進之效果。遭受外傷時,身體大部份 能量會優先進行傷口癒合Μ減少進一步的感染 ,之後再逐漸轉移至免疫糸統。CLA是目前唯一 同時具有增強免疫糸統及抵抗因免疫剌激之分 解效應(cat - abolic effect)的飲食因子° Ross (1993)亦指出,CLA可使部份的免疫细胞及其分 泌物增強,如增加I L - 2之分泌量及T細胞之活性 4. CLA對體重控制與生長促進之影響 200425837 人體服用C L A Μ後,體脂肪相對於瘦肉組織會降 低,且縮減的是脂肪細胞的脂肪容量而非脂肪 细胞數。CLA在體重控制上的其他效應還包括提 升代謝率、降低食慾、稱定減肥者的情緒,避 免悠悠(yoyo)復胖症等(Azain e t a 1 · 2000 :Pa "iza (1999) pointed out that linolenic acid can inhibit lipid peroxidation and inhibit the production of prostaglandins, and thus has an immune boosting effect. In the case of trauma, most of the body's energy will preferentially perform wound healing and reduce further Infection, and then gradually transferred to the immune system. CLA is currently the only dietary factor that simultaneously enhances the immune system and resists cat-abolic effects. ° Ross (1993) also pointed out that CLA can Enhance some immune cells and their secretions, such as increasing the secretion of IL-2 and the activity of T cells 4. The effect of CLA on weight control and growth promotion 200425837 After taking CLA M in human body, body fat is relative to lean tissue Will reduce, and reduce the fat capacity of fat cells rather than the number of fat cells. Other effects of CLA on weight control include increasing metabolic rate, reducing appetite, determining the mood of weight loss, and avoiding yoyo obesity Et al. (Azain eta 1 · 2000:
Kr itchevsky , 2000) ° Cook e t al〇(1993)認為, CLA可降低內毒素所造成之生長障礙,因而可 促進生長。 本發明之產品特性:共軛亞麻油酸具有抗癌 、預防心血管疾病之臨床翳學證明,另外亦有提 髙免疫力及控制體重之功能,食物中之共親亞麻 油酸來源主要為動物性產品,特別是反芻動物產 品,如:牛奶、乳酪與肉類含量相對較多*大咅P 份的抗癌物質都是植物性物質且含量不高,因此 共輒亞麻油酸顯得極為特別,它是自然存在的抗 癌物質,因此藉由反芻動物生產乳品而取得此項 機能性物質,Μ促進人體健康。 〜7Kritchevsky (2000) ° Cook e tal0 (1993) believes that CLA can reduce growth obstacles caused by endotoxins and thus promote growth. Product characteristics of the present invention: Conjugated linoleic acid has clinical evidence of anti-cancer and prevention of cardiovascular diseases, and also has the function of improving immunity and controlling weight. The source of linoleic acid in food is mainly animals Sexual products, especially ruminant products, such as: milk, cheese and meat are relatively high. The anti-cancer substances in P 咅 are all plant-based and the content is not high, so linolenic acid is very special. It is a naturally occurring anticancer substance. Therefore, this functional substance is obtained by ruminant production of dairy products to promote human health. ~ 7