TW200403986A - A composition including isoflavones refined from plant - Google Patents

Abstract

A composition is prepared by extracting phytochemicals from plant matter. This composition is enriched preferably in isoflavones, lignans, saponins, catechins and phenolic acids. Soy is the preferred source of these chemicals; however, other plants may also be used, such as red clover, kudzu, flax, and cocoa. The composition is a dietary supplement for treatment of various cancers, pre-and post-menstrual syndromes, and various other disorders.

Description

200403986 V. Description of Invention (1) This formal application replaces the provisional application No. 60/0 6 0, 5 4 9 of October 1997 (Republic of China). The invention relates to plant extracts, in particular to phytochemicals, including: phytosaponin, phytosaponin, catechin, lignose, phenolic acid and isoflavones; especially extracted from soybean, flax, tea , Cocoa and other plants; and methods for using such compositions as nutritional supplements or food supplements.

It is well known that plant matter contains several types of low molecular weight organic compounds that are biologically active in all types of animals. Traditionally, such compounds have been considered non-nutritional, but recent scientific evidence indicates that these substances are used for health maintenance, chemoprevention, and alleviation of some conditions or diseases related to the sex hormone cycle including sleep disorders and vaginal dryness Can play an important role. Edible plants in the general diet or substances used as herbal remedies / supplements may contain structurally related compounds. Compared with other plants, these related substances are often special in terms of quality and distribution. These biologically active compounds are best known as flavonoids, isoflavones, phytosaponins, xylose, alkaloids, catechins and phenolic acids.

Epidemiological studies of disease-related diets have identified certain dietary ingredients that can reduce the risk of human infection with certain diseases. Orientals eating soy can reduce breast cancer, prostate cancer, colon cancer, and coronary heart disease; Finns also have a lower incidence of prostate cancer. Researchers are currently investigating specific compositions in food to understand the epidemiological observations. A variety of plants eaten in the diet, several are rich in plants

Page 6 200403986 V. Description of the invention (2) Chemical = thing. Da Dan products contain high amounts of isoflavones and plant food grains, such as: barley, psyllium, and rice. Unrefined 3 xylose. Cocoa contains catechin and phenolic acids. So these two Asian chefs

I: The source of chemical molecules, such as c of kudzu plants, sugars and isoflavones. Lignose and isoflavones can be used as lignin in binocular. Tea plants are also rich in phytochemicals: J hormones and acids. 3 Includes catechin and phenol. The use of isoflavone g alone can treat or prevent milk and colon hemorrhoids as a suspect ...: Ying, M adenocarcinoma, skin cancer, Sini A as a mechanism inhibitor. Separate use of preventive toilet M tH buckle ~ ^ co-cultivation _ can also reduce or

Prevention of various symptoms related to the beginning and duration of the 彳 τ menstrual period J ^ hot Hashes and osteoporosis. Li, 廿 群, dagger burst

Si encompasses heart disease in specific vascular applications, which lowers cholesterol-month, month, month, and month, regulates angiogenesis, and other blood vessel effects. Furthermore, the use of -S together with S-S alone can reduce headache, dementia, inflammation, and aging alcohol 'while also enhancing immunity.

Lignin alone can also prevent or treat breast cancer, prostate cancer, and colon cancer. It can also reduce paroxysmal fever, prevent osteoporosis, and show resistance to filter viruses. Lignin can also resist cell mitosis and With bactericidal effect, a plant xylose called catechol-dihydroguaiaretic acid has been used as an antioxidant by the food industry. Phytosaponin alone can prevent or treat skin cancer, colon cancer, reduce blood cholesterol, and enhance immunity and antiviral activity. Phytosaponins also show antioxidant effects and can act as free radical scavengers. Acid tests have shown antioxidant activity.

Page 7 200403986 I. Explanation of the Invention (3)-

I | People who eat high soy content ’can reduce many of these types of symptoms. ^ This shows that eating a diet containing a certain proportion of phytochemicals 丨 compounds found in soybeans may lead to additional or synergistic effects. However, high-soy diets have some adverse effects on Western consumers, such as gastrointestinal flatulence, bad taste, and hesitation, and they have changed their lifestyles to include soy in their diets, even if they are for these benefits. Currently known, isoflavones which are isocyclic phenols include: soybean compounds 5,7,4-trihydroxy isoflavones (2611 丨 51: 丨 11), soy bean syrup ((1 & 1 (12111), glycin glycitein, biochanin A, equiol, formononetin, o-desmethylangolensin and natural derivatives. When these compounds are consumed These compounds, as well as sugar aglycones or demethyl aglycone compounds, such as 5,7,4-trihydroxy isoflavones (or genistein), have similar activity to soy glycogen. And they have It is called phytoestrogens. Xylose is defined as a compound containing 2, 3 -difluorenyl butane structure. It includes: mathansi (matairesi η ο 1), secoisolariciresinol , Lariciresinol, isolariciresinol, nordihydroguaiaretic acid-pinoresinol, olivn and other possible enteric lactones And intestinal glycol precursors and mutations ., _ Which comprises a double-furoic acid glycosides include Fun - hydrogen benzoic acid (p-hydrobenzoic acid), the original child

Page 8 200403986 V. Description of Invention (4).

Protocatechuic acid and vanillic acid. Other acids are chlorogenic acid, caffeic acid, ferulic acid, gallic acid, sinapic acid, syringic acid ), Coumar ic ac id, cinnamic acid, gen tisic acid, salicylic acid, hydroxy benzoic acid, hydroxybenzoic acid phenyl acetic acids) and its derivatives. Phenolic acids should also cover various isomers and derivatives from natural fruits and vegetables. Catechin or flavanols include: epigallocatechin, catechin, epicatechin, and gallocatechin.

Saponification is a steroid containing 27 carbons (C-2 7), and its branched chain is metabolized to produce a spiral ketal. The naturally occurring saponin is phytosaponin, which is a 3-0-glucoside with steroids or triterpenes as the main component. Digitalis saponin in foxglove is plant saponin. Phytosaponins include: saponin glycosides such as three types of steroids or steroids; and sugars such as glucose, arabinose, galactose, and uronic acid. The typical leguminous saponin is: Isobaric! Glycyrrhiza glabra, hay soap glycyrrhizin (giyCyrrhetinic acid + glucuronic acid), soy saponin in soybean, and alfalfa phytosaponin (alfalfasaponin) in Medica sativa. Phytosaponins still contain chemical entities that have been identified as triterpenoids, such as: tom atine, soybean saponin phenol 200302986 V. Description of the invention (5) ^ A, B, C, D, E And F (soyasapogeno 1 s A, B, C, D, E and F), people

I * I 丨 sensengoside fractions 3 and 4, alfalfa 丨 Medicagenic acid, hederagenin I, glycyrrhi z i n

I digitonin), quillaj a saponin, lucernic acid, zahnic acid. Natural modifications of the above-mentioned compounds contained in plants are also included herein. The required improved composition, which generally includes isoflavones, xylose, saponification, phytosaponin, and / or phenolic acids, will produce a better effect than taking a single substance. In addition, there is a need for a composition that is richer in effective phytochemicals than natural ingredient concentrations. This allows individuals to easily consume such plant chemicals as a nutritional supplement or food additive. The object of the present invention is to provide a convenient way for the general public to provide isoflavones, xylose, phytosaponins, catechins, and / or phenolic acids as nutritional supplements or traditional food ingredients. Another object of the present invention is to provide the optimal phytochemical extract composition to consumers, and deliver sufficient concentration for easy taking such as: pills, tablets, capsules, liquids or food ingredients. Yet another object of the present invention is to prepare a phytochemical extract as a cream or lotion for local use. In this form, isoflavones, xylose, phytolusin, catechin and / or phenolic acid are suspended in a suitable liquid or gel to provide a stable cream or lotion for use. I Yet another object of the present invention is to provide an extract concentration, its chemical group |

It is very similar to the chemicals found in natural plants. I

Page 10 200303986 V. Description of the invention (6) In keeping with the above-mentioned invention concept, isoflavones, xylose, phytosaponin, catechin and / or phenolic acids are all extracted from appropriate plants to provide a more natural substance concentration Higher composition, and one or more of the biologically active substances are five times higher than natural substances. This extract can be used alone or in combination with one or more other plant extracts to produce the optimal combination. In addition, the extract composition can be combined with one or more nutritional agents, such as vitamins, minerals, amino acids, etc., to provide an optimized nutritional supplement that can promote desired health effects.

All of the above ingredients can be combined with the necessary binders, excipients, preservatives, pigments and other substances commonly used in the industry to produce appropriate tablets, capsules, pills, liquids, creams, powders or food ingredients. These phytochemicals can be packaged and supplied in a manner well known in the supplement and food ingredient industries. These substances are primarily intended to provide health and well-being.

The improved composition can be obtained by fractional distillation containing high amounts of isoflavones, xylose and other phytochemicals such as defatted soybean flakes, soybean molasses, soybean milk, red clover, alfalfa, flax, cocoa, tea, or pueraria. These can be fractionated separately or fractionated with other plants known to be high in isoflavones, xylose, phytosaponins, catechins and phenolic acids. Fractionation results remove large amounts of water, carbohydrates, proteins, and fats from the source material. This fractionation method is preferably an extraction method disclosed in U.S. Patent No. 5,702,752 or U.S. Patent No. 4,428,876, or ethyl acetate or η-butyl S? U.S. Patent No. 5,702,752 is assigned to the assignee of the present invention. Other extraction methods that can be used individually or in combination include the solubility difference method, steam distillation method, solvent extraction method, adsorption method, molecular weight difference method, and

Page 11 200403986 I. Explanation of the invention (7) Precipitation method. The preferred composition is an improvement on a known commercial product, mainly the amount of phytochemicals per gram and the amount of different plant chemical substances that affect physiological functions. These natural substances have been eaten for a long time, and in particular they are related to the intake of health benefits, which provide infectious disease evidence. In addition, other benefits are derived from related phytochemicals whose physical properties have been improved, and such phytochemicals are derived from their original food source. The obtained composition preferably includes 5-95% of iso-yellow, 3%, 0-70%

Lignin, 2-70% saponin and phytosaponin. Another preferred form is from soybeans. Another preferred form is that the composition includes a ratio of (phytosaponin + saponification) to isoflavones between 1: 100 and 100: 1, wherein the isoflavones are mainly 5, 7, 4-trihydroxy Isoflavone natural derivatives and / or its progenitors chitosan A and daidzein and / or its precursors formononetin, and 5,7,4-trihydroxy isoflavone The ratio to the soybean bean glycogen derivative is between 1 0 0 · · 1 to 1: 1 0 0. Preferably, the isoflavones are mainly glycosyl derivatives.

The ratio of this composition can be easily changed by changing the plant source or combining extracted plant sources. Therefore, further research shows that the combination of different phytochemicals is more effective for certain health conditions, and this particular composition will also change. Isoflavone, xylose and phytosaponin are known to be extremely helpful in the treatment or prevention of various types of cancer, including breast cancer, prostate cancer, skin cancer and colon cancer. ! It is generally believed that this improved composition can provide great benefits in a chemopreventive manner

Page 12 200403986 I V. Description of the invention (8) ^ Assistance. And recent experiments have confirmed this belief. _ Example 1 A series of preliminary animal experiments were performed to understand the effects of edible soy products on the growth of SCI D male rats implanted subcutaneously with LNCaP. High Isoflavone Content Soy Protein Isolate (SPI) (2 mg isoflavone / g SPI) was provided by Protein Technology International, Missouri, USA. A Dahli phytochemical extract is a soybean phytochemical concentrate (SPC) containing 28.5% soy isoflavones and various amounts of other soybean plant compounds, and is provided by Archer Daniels Midland Company of Illinois, USA. These materials were used to prepare six groups of experimental foods. Table 1 shows the composition of the food. Male 8-week-old SCID rats were injected subcutaneously with 2 X 1 0 6 LNC aP cells taken from the host, and the male rats were randomly divided into six groups (n = 1 ^ and each was given a set of experimental food) At the end of the experiment, blood samples were collected, and serum was isolated for p-eight analysis. One part of the tumor tissue was fixed with formalin, and paraffin coated altar and trowel were used to form 4 m ra Immunohistochemical analysis of> Zhou Zhou cells (a ρ ο pt 〇ticce 1 1) in situ, sacrifice and weaving, knife set and angiogenesis and reproduction. Enemy clams, and Buba were prepared into cells The solution was used for western transfer to determine the apoptotic phase-knife product. Gene Table 1 sorted out the effects of soybean products on food intake, body weight, yellowing, and tumors. Soy products did not affect food intake or body weight. Compared with the control group of protein protein and silver food, the treatments were treated with sp factory modification (200 / 〇), SPC (1.0%), and SPI and spc (h 〇0 / 〇).

Page 13 200403986 I V. Description of the invention (9) "-~ The amount of reduction is 12%, 28% (ρ < 0.04) and 40% (p < 〇〇〇05). The analysis results showed that the protein source had no significant effect on tumor growth. Linear regression-I analysis showed that the amount of tumor was inversely proportional to the amount of isoflavones in the diet (tumor volume (cubic 丨 cm)-〇〇〇〇〇 + 2.121χ Isoflavones (mg), r2 = q 76, p < 0.03) 〇 Table 3 shows the effect of spc control at 1.0% diet on apoptosis, weight loss, and blood regeneration. It shows that food additives of soybean phytochemicals can promote cancer cell death and inhibit tumor cell proliferation to inhibit LNCaP tumors growing in vivo. Its inhibitory effect on tumor angiogenesis is not obvious, probably because the number of samples is too small (n = 2). Test tube experiments have shown that 5,7,4-trihydroxy isoflavones and soybean phytochemical concentrates can inhibit PSA secretion by LNCaP cells into the medium. When treated with 5,7,4-trihydroxy isoflavones at 25 and 50 mm, the P S A concentration can be reduced by 6 8 ° /. And 74%; and when treated with 25 and 50mm soybean phytochemical concentrates, PSA can be reduced by 31% and 42%, respectively.

Page 14 200303986 III. Description of the invention no) Table 1. Experimental food ingredients (: grams) with 1 casein with 2 SPI formula 3 casein / LSPC with 4 SPI / LSPC formula 5 casein / HSPC with 6 SPI / HSPC SPI 0 200 0 200 0 200 200 Casein 200 0 200 0 200 0 0 DL-Synthetic acid 03 3 3 η 3 Corn starch 150 150 150 150 150 150 Sucrose 500 500 500 500 500 500 Cellulose, BW200 50 50 50 50 50 50 Corn oil 50 50 50 50 50 50 50 Mineral mixture, S10001 35 35 35 35 35 35 Vitamin mixture, V10001 10 10 10 10 10 10 Choline ditartrate 2 2 2 2 2 2 Soy plant compounds 0 0 2 2 10 10 Total (g) 1000 1000 1002 1002 1010 1010 (Isoflavones, mg / kg food) 0 245 341 586 705 950

1 ·· Dr.E.A.Ulman Research Diets, Inc · Slightly modified from AIN formula

Page 15 200303986 V. Description of the invention (11) Table 2 Final weight, total food intake, total isoflavone intake and tumor volume ΤΓΠ weight food intake g / m total isoflavone volume tumor volume (cm3) casein 22.4 soil 0.5 丨 46.6 ± 3. '0.00 ± 0.00 2.32 ± 0.3Γ SPI 23.1 ± 0 · 7 46.2 ± 2.8 1.7 (30 ± 6.37 2.06 ^ 0.32 casein / LSPC 21.U 0.7 41.2 ± 3.4 14.03 ± 14 1 · 88 soil 0.35 SPI / LSPC 22.6 people 0.6 50.1M.7 29.36 people 2.76 1.66 people 0.29 'casein / HSPC 22_2 people 0.7 44.8 people 6.1 76 · 38 people 10.40 1.6 0.22' SPI / HSPC 22.0 people 0.6 47.5 people 1.7 92.53 people 3.22 1.39 dirt 0.30 "

丨 2: The value is the average I standard deviation-it means that there is no significant effect on food intake or body weight.丨 3: Compared with the control group, SPI / LSPC, casein / HSPC and SPI / HSPC | Significantly smaller tumor volume (*: P < 0 · 04; **: PC0.005) 丨 Table 3 pairs of eyebrow death indicators (Person 1,% 1 ^ heart 1), reproduction index (? 1,%?)

Treatment AI (% TUNEL) PI (% PCNA) Microvessel density control group (n = 2) 6.07 ± 0.88 60.1 ± 1.1 12.5 ± 3.8 Casein / HSPC (n = 2) 10.75 ± 0.54 51.7 ± 1.3 9.7 ± 0.7 P値 < 0.02 < 0.01 > 0.05

16 I 200403986 V. Explanation of the invention (12). The values are the average value ± the standard deviation. In summary, the preliminary results show that soybean products can inhibit the subcutaneous growth of _ LNCaP tumors in SC ID male rats, possibly because of the induction of apoptosis procedures, and Inhibits angiogenesis and proliferation. Isoflavones or xylose can reduce symptoms associated with menopause, such as paroxysmal fever and osteoporosis, and alleviate symptoms during menstruation. And its further inference is due to the activity of estrogen. Therefore, the improved compositions described herein are more effective in reducing these symptoms. In addition, isoflavones also have positive effects on cardiovascular-related symptoms, such as heart disease, cholesterol (phytosaponin also has a positive effect on cholesterol), angiogenesis, and other vascular symptoms. It is believed that the improved composition will reduce cardiovascular disease as well as the methods known so far but at a lower cost. As mentioned previously, it is known that isoflavones, xylose and phytosaponins each have positive effects on neurological and immune symptoms. It is believed that the improved composition will reduce neurological and immune symptoms and have the same effect as the methods known so far. , But the cost is lower. In addition, the combination described in this article can be expected to assist. Take the modified composition orally or by injection or parenterally, such as subcutaneously, intravenously, intramuscularly, intraperitoneally, or intranasally or sprayed into the mucosal tissue, or as an ointment or cream Apply to skin. Modified compositions can be taken in any suitable vehicle in solid or liquid form, such as lozenges, capsules, powders, soft gels, liquids, suspensions, emulsifiers, ointments or emulsions. The improved composition can also be taken as a food supplement or supplement.

Page 17 200403986 丨 V. Description of the Invention (13)-| The dosage of the improved composition depends on the individual, the way of taking and the desired effect. A generally effective dose is 10-2000 mg / dose. I Example 2 Tablet Manufacturing! The composition provided by this patent can be used to prepare tablets or other servings. Example 2 provides an example of capsule preparation. The higher the active ingredient concentration, the easier it is to form a sheet or latex. This property allows other nutrients to be added to it. One example is the preparation of a phytochemical tablet containing calcium and vitamin E as a means of maintaining bone health and / or reducing symptoms such as paroxysmal fever after menopause. In the example of this embodiment, a 600 mg dry compressed tablet containing 125 mg of isoflavone concentrate (50 mg of isoflavone compound) was prepared. This tablet contains calcium and magnesium. The production of dry compressed tablets first put the following ingredients in a 120-quart Hubble (Η 〇bart) mixer and stir. · 4 kg of the improved composition (39.83% isoflavones), 1 91 kg of sorbitol, 0.95 kg of magnesium stearate, 13.1 kg of dicalcium phosphate. After the stirring is completed, it is compressed into a sheet by a Stryker BB2 simple compressor at a pressure of 1 ton. Each tablet weighs 600 mg and contains 125.53 mg of the improved composition, so it contains 50 mg of Total isoflavones. Alternatively, a phytochemical concentrate can be provided in a single dose, such as a skin lotion or a food ingredient added to traditional foods at a dose of 10 to 2000 mg / dose for the purpose of promoting health and well-being. Benefits include cancer prevention, estrogen and sex hormone-related diseases, suppression of the pituitary-thyroid-gonadal axis, reduction of alcohol dependence, regulation of the cardiovascular system, immune and nervous system, anti-filter pathogenic effects and analgesic effects.

P.18 200303986 V. Explanation of the invention (14) »Example three | Two-piece animal capsules are prepared by filling 0.106 g of the modified compound (44.35% isoflavone) into one half of the 0-shaped empty capsule, and Cap tightly with the other half of the capsule, which provides 47.2 mg of total isoflavones.

I Example 4 Table 4 shows a comparison of different sources of phytochemicals. It shows that the phytochemistry of the composition of the isoflavone concentrate of the present invention is much higher than that of ordinary natural fruits and vegetables. Obviously, the content of glucoside isoflavones glucose and phytosaponin is more than 100 times higher than that of food sources, and more than 20 times higher than that of young shoots of plants that can naturally concentrate such phytochemicals. Compared with other concentrates, the 'isoflavone concentrate' of the present invention shows that isoflavones are the dominant component of the latter product, which will reduce other health-friendly phytochemicals. In addition, the extraction technology of other products uses technology that changes its composition (especially isoflavones), so it is not exactly the same as that in natural fruits and vegetables (US Patent No. 3,637, 562). An improved composition combined with other previously described combinations The results are shown in Table IV.

Page 19, 200403986 V. Description of the invention (15) Table 4 Comparative product samples of the present invention Product samples isoflavone sugar products (mg / g) Isoflavone suspension products (halo g / g) 5,7,4-trihydroxy isoflavone -Keto / soybean glycogen ratio 1 xylose (milli: g / g) Phytosaponin (mg / g) Phenolic acid (mg / g) Improved composition 401.1 3.37 1.06 to 1 0.2 460.7 25.47 Soy 1.748-2.77 6a 2.776 0.07 5 1.59-2.7 NA 0.9-3.2 ° Soy flour (non-fat) 1.969a 0.045a 3.58 0.0013 2.870c Soy sprout 24.32d 0.85d NA 16.7-1.985 ΝΑ Patented product ε (PTD ΝΑ 2.5-6.5c 0.5-3.5 NA NA ΝΑ patented product f (PTI) NA 5.M4.7f 0.433-3.48 NA NA ΝA patented product 2 (PTI) NA 1.7-3.52 0.66-2.86 NA NA ΝΑ ΡΙΙ h h ΝΑ 970 12.8 NA NA ΝΑ ΡΙΙ h h ΝΑ 640 2.0 NA NA ΝΑ Soy molasses (dry) 27.6 0.1 1.37 NA NA 5.788 Novogen: 0.0 550 1-1.7 tol NA NA ΝΑ

a ·· W a n g Η · and Mu r p h y P. A., Journal of Agricultural Food Chemistry, 1 9 9 4, 4 2, 1 6 6 6-1 6 7 3,

Page 20 200403986 V. Description of the invention (16) _ b: Anderson RL · and Wolf WJ ·, Journal of Nutrition, 125: 581S-588S, 1995 'c: Seo A · and Morr CV ·, Journal of Agricultural Food Chemistry, 1 984 , 32, 530-533. D: Soy LifeTM promotional literature e: WO 95/10530, PCT / US94 / 10697 e: WO 95/10512, PCT / US94 / 10699 e: WO 95/10529, PCT / US94 / 10696 h : NCI document i: Novogen propaganda document Example 5 This improved composition contains isoflavones in the form of furoside, which has a higher solubility at body temperature than the sugar-containing per-lipid composition described previously. A 5,7,4-trihydroxy isoflavone (genistein), genistin, daidzein (diadzin) and isoflavone concentrate were prepared from a measuring bottle. The sample was placed in a water bath at 37 ° C for 17 hours, and then quickly filtered with a 0.2 micron syringe filter to remove particulate matter. The filtrate was analyzed for isoflavone concentration by high performance liquid chromatography (HPLC). The results are shown in Table 5.

Page 21 200303986 V. Description of the invention (17) Table 5: Comparison of solubility of isoflavone glycosides and aglycones. Isoflavone samples 5,7,4-trihydroxy isoflavones (ppm) Genistein (ρρπ0 Soya Bean Primitive) (ppm) Soy Bean (ppm) 5,7,4-trihydroxy isoflavone 7.42 Genistein 33.89 Soybean Λ original 3.64 Daidzein 48.51 Isoflavone concentrate 0.492 30.075 0.672 37.69 Glycosides and daidzein, in The concentration at 37 ° C is at least 4.57 and 13.32 times higher than the relative aglycone form, respectively. |

i I

The change to isoflavones is to remove carbohydrates around the isoflavones. This I 1 i

A change made the isoflavones less soluble in water. Maintain natural change, sugar base as I

; I 丨 use, can promote solubility. This fact is shown in Table 5 for the solubility ratio 丨 j t 丨 comparison. Compared with the relative non-glycoside form, the dissolution of genistein isoflavones is 4.6 times higher, while daidzein isoflavones are 13.3 times higher. The high solubility makes the intestinal organisms have better bioavailability. Glycosylation does not inhibit its absorption in the intestine, because bacteria in the intestine can convert the glycosidic form to the glycosidic: aglycone form before absorption. Example 6 < Xylose can be extracted from flax by the following methods. 9 78 g of defatted flax meat (F1) was extracted with 2000 g of 85% ethanol at 40 ° C for 10 minutes to form a mud. After filtering the mud, it was repeatedly extracted twice with 60,000 g of alcohol.

Page 22 200403986 V. Description of the invention (18) ^ The alcohol part is volatilized in a vacuum state of 70 3C to form 1 186 g of liquid substance. This liquid was mixed with 1,000 grams of water. The above-mentioned mixture has a molecular weight separation membrane of 50000, and a filtrate of 767 g and a filter residue of 123 g are formed. After the filtered part was dried by cold beam, a sample of 2 7 · 84 g (F 2) was formed. The 7 6 g portion of the filtrate was poured into a 35 mL X A D-4 resin column at 50 ° C at a rate of 10 mL per minute. The effluent was collected and dried to form 14.8 grams of sample (F3). XAD-4 is a brand name of absorbent resin, purchased from

The Rohm & Haas 〇 column was rinsed with 70% ethanol in four volumes (140 ml) at 50 ° C. The eluate was evaporated to dryness under vacuum at 70 ° C to form 1.79 grams of sample (F4). The lignin content analysis was performed for four parts and the concentration was calculated based on the weight of secoisolariciresinol. As shown in Table 6, this extraction method can increase the concentration of xylose. Table 6: Lignin Concentrations (Calculated with L-Isolanin) Partial F1 F2 F3 F4 Iso-Larisin Concentrations (mg / g) 2.3 1.9 4.8 13.4 Phenolic acid Although the present invention has published preferred embodiments to assist Know the φ of the present invention, but the present invention can also have various embodiments without departing from the principles of the invention. Therefore, the present invention should cover all possible embodiments, improvements, and examples equivalent to the embodiments without departing from the invention principles of the scope of the attached patent application. 200403986 Schematic description

Claims (1)

200403986 6. Scope of patent application 1. A method for providing dietary supplements, which can be used to prevent or ameliorate diseases selected from the group consisting of breast cancer, skin cancer, and intestinal cancer. The method includes providing a plant extract composition It also helps human health. The composition contains at least two or more refined phytochemical distillates selected from the group consisting of isoflavones, xylose, phytosaponins, catechins, and phenolic acids. 2. The method according to item 1 of the scope of patent application, wherein the method can be used to treat breast cancer. 3. The method according to the scope of patent application, wherein the method can be used to treat skin cancer. 4. The method according to item 1 of the patent application scope, wherein the method can be used to treat bowel cancer. 5. A method of providing dietary supplements, which can be used to prevent or ameliorate diseases selected from the group consisting of urinary tract cancer, bladder cancer and prostate cancer, the method comprising providing a plant extract composition and helping the human body In the step of health, the composition includes at least two or more refined phytochemical distillates selected from the group consisting of isoflavones, xylose, phytosaponins, catechins and phenolic acids. The method of item 5, wherein the method can be used for treating urethral cancer. I I 7. The method according to item 5 of the patent application, wherein the method can be used for the treatment of bladder cancer. 8. The method of claim 5 in the scope of patent application, wherein the method can be used to treat prostate cancer. 9. A method of providing dietary supplements that can be used to prevent or improve Page 25, 200403986 6. Patent application: Disorder disorders in the family consisting of migraine and dementia. The method includes the steps of providing a plant extract composition and helping human health. The composition contains at least two types of extraction. The phytochemical distillate is selected from the group consisting of isoflavones, xylose, phytosaponins, catechins and phenolic acids. 10. The method according to item 9 of the scope of patent application, wherein the method can be used for treating migraine. 11-The method according to item 9 of the patent application, wherein the method can be used to treat dementia. 1 2 · A method for providing a dietary supplement, which can be used to prevent or improve alcoholism, the method comprises the steps of providing a plant extract composition and helping human health, the composition comprising at least two kinds of refined plants A distillate of a chemical substance, which is selected from the group consisting of isoflavones, xylose, phytosaponins, catechins and phenols. 1 3. A method of providing dietary supplements, which can be used to prevent or reduce cholesterol in the blood, reduce the risk of coronary heart disease, or regulate blood fat, the method includes the steps of providing a plant extract composition and helping human health The composition contains at least two or more refined phytochemical distillates selected from the group consisting of isoflavones, xylose, phytosaponins, catechins and phenolic acids. 14 · The method according to item 13 of the scope of patent application, wherein the method can be used to reduce cholesterol in the blood. 15. The method according to item 13 of the scope of patent application, wherein the method can be used to reduce the risk of coronary heart disease. 1 6. The method according to item 13 of the patent application scope, wherein the method can be used as a debugger Page 26 200403986 I Sixth, the scope of patent application-blood fat status. 17. A method of providing a dietary supplement that can be used to reduce or prevent paroxysmal fever, osteoporosis, sleep disorders, vaginal dryness or premenstrual symptoms, the method comprising providing a plant extract composition and helping For human health, the composition includes at least two or more refined phytochemical distillates selected from the group consisting of isoflavones, xylose, phytosaponin, catechin, and phenolic acid. 18. The method according to item 17 of the scope of patent application, wherein the method can be used to prevent or treat red tide. 19. The method according to item 18 of the scope of patent application, wherein the method can be used to prevent or treat osteoporosis. 20. The method according to item 18 of the scope of patent application, wherein the method can be used for preventing or treating premenstrual symptoms. 21. The method of claim 18, wherein the method can be used to prevent or treat sleep disorders. 2 2. The method of claim 18 in the scope of patent application, wherein the method can be used to prevent or treat vaginal xerostomia. Page 27 200403986 VI. Designated Representative Map