TW200307500A - Products comprising solid culture of Zang Zhi, and use thereof - Google Patents

Abstract

The invention provides the solid culture of Zang Zhi obtained by an incubation method for obtaining solid culture of Zang Zhi (Antrodia camphorata), and the products processed therefrom and the use thereof.

Description

200307500 发明 Description of the invention: TECHNICAL FIELD The present invention provides a solid culture of Antrodia camphorata, its product and use. Previous technology Zang Zlu (Zang Zlu), its scientific name is Antrodia camphola, also known as Antrodia camphora, Antrodia camphorata, Antrodia camphorata, Antrodia camphorata or Antrodia camphorata, a fungus peculiar to this province, which grows only in the Antrodia camphorata ( Cinnam〇mum kanehirai) 's hollow body's inner wall' fruit body has a strong camphor tree aroma, and its shape is plate-shaped or bell-shaped. Folklore has soothing effects, timidity, ulcers, blood circulation, detoxification and detoxification, and sedative pain relief. It is a good antiseptic and antidote, and is regarded as the most expensive wild fungus on the Taiwan market. What's more, it is different from the indirect effect of Ganoderma lucidum to improve immunity and anti-cancer effect. Antrodia camphorata is a mechanism that directly inhibits or kills cancer cells. The effect of activity. In particular, Antrodia: treats gastrointestinal pain, diarrhea, vomiting in zone D, diabetes, gout, nephritis, arthritis, hair irritation, allergies, high urinary protein, uremia, cirrhosis, liver cancer, and influenza. " Numerous research units have invested manpower and material resources to study the active ingredients of Antrodia cinnamomea 'The research project of the National Science Committee of Langzhong Executive Yuan uses chemical methods, nuclear magnetic resonance optical analysis (NMR), and known compounds + spectra Comparison to establish the structural characteristics of the active ingredient compounds of Antrodia camphorata: The research results of the active ingredients of Antrodia camphorata indicate that the mention of methanol extracts in Antrodia camphorata inhibits the growth of Staphylococcus aureus and P. acnes

〇 · Λ87 \ 87623 DOCMAN 200307500 Using zankuic acid (Zankuic acid), which is an alcohol extract of Antrodia camphorata, has significant inhibitory effect on platelet agglutination and platelet aggregation in p-3 88 mice; while camphoric acid B has Weak anti-biliary test and anti-brain amine (serotonin) function. Gao Xiaowei's master's thesis research on the composition of the lingzhi of the new species of Ganoderma lucidum in Taiwan pointed out that Ganoderma comphoratum Zang et Su is a new species of Ganoderma lucidum published in 1990. The fruit body of Antrodia camphorata was extracted with acetone to obtain a crude extract. Analytical method (LC, PLC) separation and purification by recrystallization; TLC Seanning and high performance liquid chromatography (HPLC) to identify the purity. Structure determination was performed via f-spectrum, infrared spectrum, ultraviolet spectrum, and nuclear magnetic resonance spectrometers (H_NMR and C_NMR). ° Among them _ has a reducing effect on the amino acid_pyrogluconate transaminase (CA) value in the serum of mice with acute hepatitis induced by carbon tetrachloride (called). Because Antrodia camphorata is mostly distributed in the broad-leaved forest area of the Taitung coast mountain range in Taiwan, according to the Forestry Bureau's Antrodia camphora conservation announcement, the heart has been designated as a nature reserve. Here, the number of Antrodia camphorata growing is very small and has been Collected. Therefore, the city is no longer able to obtain natural wild Antrodia. The main purpose of Ke = Ming is to provide a solid cultivation method of Antrodia camphorata. The second method is to cultivate a solid substance of Antrodia camphorata that has the same pharmacological effects as natural wild Antrodia camphorata. Another object of the present invention is to provide a solid culture obtained by cultivating a foodstuff deposited in Taiwan with a g number of CCRC 35398 as a source, and cultivating it by a solid surface cultivation method. O: \ S7 \ 87623 DOQLan 200307500 Another object of the present invention is to provide the present invention

Processing of solid cultures Another use of the invention. The present invention provides various activities of the solid culture of the present invention. The invention provides a solid cultivation method of Antrodia camphorata, with the aim of artificially cultivating large-scale cultivated fruit bodies with natural wild Antrodia camphorata ingredients and activity. The present invention is a solid cultivation method of Antrodia camphorata, which comprises cultivating Antrodia camphorata into a fibrosus culture in space, and continuing to cultivate fruit bodies in the air. Before the mycelium culture stage, the proliferation step can usually be performed first. For example, the following steps can be used to prepare a large number of g species for space bag culture. 彳 Contains three steps: 取 take from liquid nitrogen preservation and activated bacterial species-small piece of agar The mycelium pieces are transferred to a fresh medium and cooked under a Shilitiao + T cellulite box, and the bacteria are allowed to grow vigorously. (2) The bacteria are inoculated to a sterilized fibrous material (such as wheat kernels or In the space packs of the wooden men, continue to culture at constant temperature; (3) When the fungus in the space pack is full of hyphae, remove the uppermost mycelium block, and then inoculate a large amount into the space pack. . Proliferation steps before the mycelium culture stage, or fermentation in liquid culture to mass culture bacteria, and then inoculated into the space bag. The formula of the fermentation culture solution is 1-3% fructose, 0 · 01_0 · 1% magnesium sulfate, 〇 ″]% yeast extract 0.05-5.5% potassium dihydrogen phosphate. Especially 2% fructose, 0.05% magnesium sulfate, 0.5% yeast extract and 0.1% potassium dihydrogen phosphate After the oblique interview tube culture, inoculate a large amount of the propagated bacteria into 5 liters of fermentation broth, and Rotate and shake at 26 ° C for 240 days at 240 rpm, and continue to culture at 90i * pm for about μ days. Then inoculate to 20 liters of liquid hair.

O: \ 87 \ 87623 DOOLAN 200307500 Stir culture in fermentation solution for 14 days. The space pack here is based on a plastic bag with a weight percentage of 70 /. Any fibrous matter such as stems, stalks, fruits, or wood chips of any quince or plant (especially the stems, stalks, fruits, or fiber sawdust of the herbaceous plant), the source of the meal (especially the sweet potato), and 5_15% of grains (especially 1 rice bran is better), H0% of sugars (especially glucose is preferred), 0.5_2% of phosphorous phosphonium = salt, (X is best to be broken down by acid dihydrogen), and 0.1- 1% sulfate (especially magnesium sulfate). Its humidity is about 60-90%, and it is better to use thorium, and adjust its acid-base value to be close to the order. The mycelium culture stage referred to herein means that the bacteria are planted into the space bag for about 60 days. The growth temperature of the mycelium is between 5 and 32C > c, especially 28, which is the optimal growth temperature. The humidity of the space bag is preferably between 6% and 60%, and the sail is better. As for its air condition, it is better to use q.2_i% carbon dioxide. 0 After the filiform culture is completed, the fruiting body cultivation stage is performed, that is, the space bag is removed in about 61 days, so that it is completely exposed to the air. . The temperature difference between day and night during the growth of fruiting bodies must be strictly controlled, and the daytime temperature is generally between M and 5%. 30 C room ’and 25 C soil _11 is the best; night temperature is preferably i 丄 ° c; day and night temperature difference is 15t. Fruiting body cultivation stage 1 = degree should be controlled between 90 · 95%; in addition, the fruiting body must be under air circulation: culture 'of which the carbon dioxide content cannot exceed 1%, generally the solid culture can be harvested within ⑽ days . _ Invention 'is a solid O: \ 87 \ 87623 D〇〇La > j 200307500 culture obtained by using the CCRC 35398 M species that is deposited with the Taiwan Food Industry Development Research Institute and compiled as ㈣, as shown in Figure 1 + ^ Ancient dogs are about A, with a cone shape, a diameter of about 10-30 cm, a degree of about 15-35 cm, and a weight of 0.2-0.6 kg. The solid objects obtained by the above-mentioned cultivation method can be processed by any familiar skill ^ ^ ^ 'If you use water extraction or alcohol to extract the active ingredients of the solid culture, choose ιν ^,焉 乂 Known concentration methods, such as vacuum concentration (vacuum conceniration) ... 7 concentration / freeze (freeze concentration) to prepare concentrated products. Or give Su Fengsheng field by drying method ... For example, spray drying, freeze-drying, etc., where spray drying without heating can get dry and powder free from heat. Especially, the cold drying method is preferred. The solid material is removed from the low temperature to normal temperature below the generation, and the solid culture is not affected by oxygen during the drying period to change its properties. Or make the spray-dried micro-powder particles or micro particles prepared by other drying methods to increase the agglomeration and particle size of the powder through agglomeration (agglomeraton). The structure of the granulated product is porous and has good Wettability. Comparison of high-performance liquid chromatography (HPLC) spectrum data of the solid culture of Antrodia camphorata in the present invention, Antrodia camphorata obtained from the conventional liquid culture method, and wild Antrodia camphorata, as shown in Figure 2. According to HPLC analysis, the fruiting body obtained by the solid culture method of the present invention has a composition very similar to that of natural wild Antrodia camphorata. When it has the same activity and use as natural wild Antrodia camphorata, including calming, timidity, stasis and blood circulation, Wenzhongxiao It has the effects of accumulation, detoxification and swelling, and sedative pain, as well as the ability to inhibit germs, viruses and tumor cells, and the effects of strengthening heart, immune regulation, 5 丄 I, parasympathetic effect, antiserotonin activity. Can be used to treat gastrointestinal pain

O: \ 87 \ 87623 DOQLAN 200307500 Diarrhea and vomiting, diabetes, gout, nephritis, arthritis, inflammation, allergies, high urinary protein, uremia, cirrhosis, liver cancer and influenza. It even has the ability to rebuild the skin, and it can also be used as a material for human skin or callus. It can be used as a patch for bedsores and skin trauma. According to the present invention, the solid culture of the present invention is further concretely demonstrated to have anti-free radical and anti-lipid peroxidation capabilities, protect liver and Wei, and inhibit cancer, ', and field cells. It is apparent that the solid culture of the present invention can be used as a healthy food for preservation, or can be further developed into various drugs. The following examples are intended to further illustrate the present invention, and are not intended to limit the present invention. Any modification and application made by those skilled in the art according to the teachings of the description of the present invention belong to the scope of the present invention. Implementation mode The bacteria collected from the Food Industry Development Research Institute under the registration number CCRC 35398 are cultured and fermented in liquid to mass culture the bacteria, and then inoculated into the space bag. The formula of the fermentation broth was 2% fructose, 0.05% magnesium sulfate, 0.5% yeast extract, and 0.10 / 0 dioxin. #The mycelium culture is carried out in the form of a space bag. The composition of the space bag is 65% of the stems, stalks, fruits and sawdust fiber of the herbal plant, 20% of yam, 10% of rice bran, 35% of glucose, and 1% of potassium dihydrogen phosphate And 0.5% magnesium sulfate, and adjusted its humidity to 80% and its value to 7. Its humidity is about 60-90%, and preferably 80%, and its ρΗ value is adjusted to be near neutral. The term “mycelium culture” as used herein refers to the time when the bacteria are planted into the space bag for about 60 days, and the growth temperature of the mycelium is between 5 and 32. 〇, especially 28t as the optimum growth temperature. The humidity of the space bag is 80%, and the air condition is 0.2% O: \ 87 \ 87623 DOCMAN -10- 200307500 carbon dioxide. After the mycelium culture is completed, the fruiting body cultivation stage is performed, that is, the space bag is removed in about 61-90 days, and it is completely exposed to the air. The temperature difference between day and night during the growth of the fruiting body must be strictly controlled. The daytime temperature is generally between 20 and 30 ° C, and the temperature between the two is 丨 丨 ^ π; the night temperature difference is maintained at 15. 〇. The air humidity of the fruiting body in the cultivation stage is controlled between 9 ... 95%; in addition, the fruiting body must be cultured under air circulation. The content of lip in the lip / lice must not exceed 90-120 days. Isoshellfish can grow to a solid culture of 15 cm in diameter and 25 cm in height, which is about 0.4 kg, as shown in Figure 1. The main active ingredient of triterpenoids is high. · The analysis of the spectrum is shown in Figure II. The values are shown in Table 1 below.

O: \ 87 \ 87623 DOOLAN -11-200307500 Table 1 丨 I. High-performance liquid chromatography data of Antrodia camphorata fruiting body obtained by the cultivation method of the present invention retains the e? W mu concentration, and the composition sot7s323L7L3r373 »7so73l3io > 7i3iQi7l3» o3! 3! 7 > 3oo330773o07003330377J0030rl30077o700o3303Q773 2 2 1 :, 丨 13!: 7V ^^: ^: ^:,;: ^ * ^: 22.23.3 :: ,,: 37. ^ :: ;;. :: ^: ^: ^. ::::: 59.35: Buckle: ^: 1: ^: 52. 12 3 < 5v, 733lolli213I4ls: 517si920212223s23s27282930313233343s36373839404142434445:; 4754950M5253s4; ss57SJ! 5061ss; 5061ss; ss67 2795ββ7〇9.3019 1000.279 ^ .53 ^ 2 6320387 2.1028 999.303 S.5299 6333234 2.1070 999.138 6.5239 1J321140 4.4984 998.38 < 6 5271 3933311 1.3087 323.263 2.1450 4614312 1.5352 303.141 1.9808 231 liter 874 0.8394 1.15 203.53. 0.5441. 4265 ^ 58 1.4132 149.458 0.9756 7601131 2.5289 460.320 3.0079 1571478 O.SS «l 71 272 0 4657 44169S 0.1463 54.184 0 0341 1264481 0.4207« 2 * 749 0.4100 1359149 0.4322 5β. S8Q 0.3834 1946283 0.6475 56.AQ7 0.3696 517351 0.1721 43.081 0.2815 5862269 1.9304 317.837 2.0769 932 ί × 0 0.1103 29.915 0.1SS5 1233931 0.4172 34.691 0.2267 538013 0.Χ790 22.β99 0. × 436 ieoia 0 0547 12.337 0.0806 1233415 0.4104 23.71β O.tS-42 428302 0.2090 1ί.25β 0.1259 12Λ9055 0.Λ 0.2442 2982581 0.59Z3 90.403 0.525Λ 2321967 0.339C 68.342 Q.4466 62734β 0.20β7 13.934 0.1303 117333 0.0390 4. ^ 90 0.030 «" 3514 0.2207 20.19β 0.1320 2932057 0.9755 52-760 〇344344 2521 ^ * 9 0.0Θ39 9. ' 3 at 0.0430 313Κ62 1.0419 79.47ί 0.5133 1X30832 0.3762 31.023 0.2027 4135840 1.37 «0 79.025 0.5098 1070268 0 3361 22.420 0.14β5 2127664 0.7079 44.217 0.28Θ9 13 ^ 97700 5.3224 304.306 1.9995 5256445 1.7488 92.365 0.« 03 9.462J 0.040 0.15 0.040 Δ49 4416830 1.4β95 79.QA9 0.3169 261569 0.0871 5.522 0.0361 441194 $ 1.-4β7β 73.32 «0.4791 2821209 0.9386 50. 0.3323 36003 0.0122 1.071 0.0070 10087950 3.35β2 134.950 0. ββ13 333401 0.1X09 4.302 0.0294 4β gold" «49 1.6291 < 0.376 0.39 ^ 5 124357 0.04K 2.047 0. 0134 49β6938 I. «391 340.488 2.220 7314754 2.433« «25.5X3 4.0874 4S86732 λ.5260 463.198 3.02S7 3699586 1.2308 189.75β 1.2399 Ι03β724 0.3515 118.120 0.7718 24« ββ79 0.8214 134.218 0.8770 1992234 1 Λ7.526 1.0233.1-44391 1-44391 0.9X37 313β € 9 «1.04-42 171.327 1 1195 1098723 0.3633 89.79-4 0.5967 1481909 0.4930 102.306 0.6699 340 ^ 697 1.1334 202.433 1.Z228 1« 45084 0 3473 104.449 0 6925 10587770 3.3225 737 281 4.9484 16ZSS63 0.5412 117.096 0 7631 336049 0.2848 79.162 0.SJ.73 1498212 0.49β4 ββ.354 0.577Z 733893 «2.4483 $ 38.4« 4 3.5182 1007 «B8 0 3351 7β.731 0.5014 ο.οαοο ο.οοοο ο.οοοο ◦.οοοο ο.οοοο ο.οοοο 0.0000 ο .οοοο ο.οοοο 0.0000 0.0000 α.οοοο 0.0000 ο.οοοο 0.0000 0.0000 0.0000 0.0000 0.0000 ο.οαοο ο.οοοοο ο.οοοο 0.0000 0.0000 ◦.οαοο ο.οοοο 0.0000 ο.οοοο 0.00OO ο.οοοOO οοοο ο.οοοο 0.0000 ο.οοοο ο.οοοο ο.οοοο α.οοοο ο.οοοο 0-0000 α.αοαο ο.οοοο ο.οοοο ο.οοοο ο.οοοο ο.οοοο 0.0000 ο.οοοο ο.οοοο ο.οοοοοοο ο αοοο ο.αοοο 0.0000 0.00 (30 ο.οοοο ο.οοοο ο.οοοο ο.οοαο ο.οοοο 0.0000 0.0000

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O: \ 87 \ 87623 DOOLAN -13-200307500 In order to compare the difference between the solids of Antrodia camphorata and other Antrodia camphorata obtained from other cultivation methods, the HPLC of Antrodia camphorata and wild Antrodia camphorata obtained by liquid culture method is shown in Figure 2. (2) and (3), the data are shown in Tables 2 and 3. It can be seen from the map that the fruit body of Antrodia camphorata cultivated by the present invention has the same peak shape as that of wild Antrodia camphorata, and it also indicates that the fruit body cultivated by the method of the present invention should have the same activity and use as that of wild Antrodia camphorata. O: \ 87 \ 87623 DOCVLAN -14- 200307500 Table 2. High-performance liquid chromatogram data of Antrodia camphorata obtained by liquid cultivation method. Retained in correspondence. * Xiangzhi No. (mr * sec > Altitude) Concentration Waterfall unit m Ingredient name 2 3 4 5 S 7 3 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 1 620 1.357 2.520 2.320 3 790 4 123 4.623 5 023 5.397 7.3βΟ 3 210 11.930 12.740 15.103 Ιβ.890 19.350 23.510 27.167 30.633 33.747 38.727 41.340 43.727 57.047 57 433 57.907 53.137 51.330 62.743 64.223 64.717 64 997 65.420 69.7333.950 66.333 79.013. 104.457 107.010 111.397 111.313 113.790 115.383 119.590 252998 3294342 5417475 1S92S34 731157 271974 154063 154230 29822 150572 123419 130807 37712 504453 143477 56360 806878 92042 144235 239 235 21124 25384 24411 2324 24411 25408 43858 25451 1 2134 38.4242 25.0369 7 341 3 3.3371 1.2599 0.7137 0.7145 0.1382 0.5975 0.5717 0.6060 0.1747 2.3370 0.6647 0.2634 3.7379 0.4264 0.6634 1.3414 1.1〇04 0.235β Q. 13Q6 0.5192 0.3140 0.4985 0.119S 0.1077 0.9108 0.1409 2.0439 0.2248 0.1859 0.3139 0.0987 0.1491 0.1363 0.10X4 0.1782 0.5863 0.2652 0 1551 0.1313 0 1177 0.2032 0.1179 38.745 650.535 726.393 99 406 23.209 11.713 7.310 4.580 1.552 5 210 3.314 3.563 1.232 11.048 3.512 1.327 11.274 1.224 2.243 3.947 3.231 0.771 0.4 70 9.096 10.275 4 508 2.279 2.024 21.767 2.094 41.520 4.370 3.529 3.823 1.S91 1.708 0.920 0.456 0-738 2.358 2.113 4.236 2.512 1.310 3.808 1.656 2.2045 37.5912 41.3301 5.6559 1.3205 0.6664 0.4444 Q.2SQ6 0.0940 0-2964 0.2227 0.2027 0.0701 0.6286 0.1998 0.1039 0.β415 0.0696 0.127S 0.2245 0.1839 0.0438 0.0257 0.3175 0.584ο 0.2565 0.1296 0.1152 1.238S 0.1192 2.3681 0.2771 0.2008 0.2175 0.0962 0.0972 0.0523 0.0260 0.0420 0.1342 0.1202 0.2410 0.1429 0 1030 0.2167 0.0942 0 0000 0.0000 0.0000 0 0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0 .0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 pvyvwvvVBBVwvvvppppppvvpppppppppppppppppppspvvvwvppvvvpvwvvwvpppppppppppppppvvvpvppvpp sum 21S86254 1757.553 0.0000 O: \ 87 \ 87623 15- 200307500 table Third, the performance of the wild stick Zhizhinan performance liquid chromatogram data compilation retention surface correction * Xiangzhi «time asked (UV- * 3« C) concentration overflow bag unit unit name 1 2 345S739 01234567830X234567390X2345678901 2 345 5 78901234567890 1111111111 ^^ 2 2 22222223333333333444444444455555555555 1.550 1.913 2.607 2.997 3.480 3.587 4.110 4.590 4 867 5.377 5.523 6.020 5.347 6.06Q 7.060 7.710 8.353 8.967 3.327 9.523 10.393 11.250 11.250 11.747 12.263 X2.837 13.733 15.300 17.513 18.270 19.363 21.567 22.567 22.537 23.537 22.537 23.537 22.537 23.537 22.537 23.537 22.367 23.537 22.567 23.537 22.537 23.537 22.367 23.537 22.367 23.537 22.367 23.567 22.537 23.537 22.567 23.537 22.537 23.537 22.367 23.537 22.537 22.537 22.537 23.567 22.537 23.537 22.567 22.537 22.537 23.537 22.567 22.537 22.537 23.537 22.367 22.537 22.537 23.537 22.367 23.537 22.537 22.537 23.537 22.537 23.537 22.367 23.537 22.537 23.537 22.367 23.537 22.367 23.537 22.367 23.537 22.367 23.537 22.367 23.537 22.367 23.537 22.367 23.537 22.537 23.537. 37.007 39.587 40.897 43.503 46.093 47 820 51.273 53.253 55 177 57 243 57 500 57.787 58 010 58 257 58.400 58 673 58.943 59 233 59.543 59.850 60.060 SO 410 1332003 12708000 7990504 3336131 827969 2100942 1565373 986357 1300 ^ 49 S20409 712514 517412 380678 622333 S57302 585765 237 1 291 547 312 1279 337 312 142 142 ¢ 999710 399151 97125 3052β12 832484 2411303 94412 950329 29686 2201S 391511 413679 3933630 4155313 3S456SS 1206951 2986313 5093407 2540255 5651787 2400643 3531633 2228945 2323969 17547630 0 57θ7 5 5210 3 4715 1 4494 0.3597 0 9704 2268 0 7235 0 7235 0.2421 0.2545 0.1200 〇1650 0.1374 0.031 ^ 0.0524 0.0251 0.0141 0.0909 0.0370 0.1175 0,0679 0.1307 0.4396 0.1295 0.1032 0.U36 0.1824 0.4406 1.9472 3 0410 0.1734 〇0422 1.3305 0.3617 1.0476 0.0410 0.4129 0.0129 009009 0 07011 0.19X9 1 7329 1.8053 t.3404 0.5244 1 2974 2.2128 U〇36 2. ^ 597 t 0430 I 3343 〇9684 1.2290 162.045 797.430 374.149 151.464 90 536 39.231 80.4ό β 61 543 59 44 5 37.086 37.566 32.737 24.939 24.053 18.364 27.106 10.957 19.904 21.157 4.914 6.584 2.283 2.199 11.437 5.603 7.438 6.542 10.369 25.904 7.175 6 715 7 129 8.1S5 20.361 103.SSO 156-204 8.733 2.428 45.150 15.926 30.354 2.102 12.943 0. S97 0 505 6.542 6.0Θ 240.883 345.611 257.829 175.620 222.955 431.601 286.541 471.025 197.347 200 -44S 164.165 335.781 994.818 1 .; 14 days 5 653S 2-6525 1.0733 0.5419 0 S326 0.5705 0.4363 0 4214 0.2629 0.2670 0.2324 0.17S8 0.1705 0-1302 0.1922 0.0777 0.14 0.0162 0- 0156 0.0811 0.0463 0.027 0.0464 0.0735 0.1337 0.0S09 Ο 047β 0.0505 0.0S79 0.1444 0.7370 1- 1074 〇 0619 0.0172 0.3272 0.1129 0.2152 0.0149 0.0918 0.0049 0.0036 0.0464 0.0431 1.7078 2.4503 1.8279 1.2451 1.5807 3.0599 2.0315 3.3394 1.3991 1.4211 1.1639 2.3006 7 0529 0.0000 OO 0000 0.0000 Ο 0000 0.0000 0.0000 〇 0000 Ο 0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 〇 0000 0.0000 0.0000 0.0000 0.0000 0.0000 〇 .0000 0.0000 0.0000 0.0000 0.0000 0.000 0 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 〇 0000 0.0000 〇 0000 0.0000 0.0000 0.0000 0.0000 〇 0000 0.0000 0.0000 0.0000 〇. 0000 0.0000 0.0000 〇 0000 〇 0000 〇 0000 0.0000 OO 0.0000 〇 0000 OO 0000

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O: \ 87 \ 87623 DOOLAN -16- 200307500 51 60.743 13833800 6.0101 994.628 7.0516 0.0000 52 SO.920 9930270 4.3142 941.167 6 S726 0.0000 S3 51 343 3601150 l 5645 265.761 1.3842 0 0000 64 51 723 6043523 2.6278 525.238 3.7238 0.0000 S5 52 127 2249 0.9774 ISO 527 1.1381 0.0000 > SS 52.357 2349680 1.2380 152.131 X 0786 0 0000 61 53.207 11335680 4.9243 336 220 6.2830 0 0000 S3 63-460 3758168 1.6327 214.761 1 5226 0.0000 59 64.087 17403430 7.5609 992.676 7.0377 0.0000 70 54.807 6379895. 55.487 1105522 0.4803 48.756 0 3457 0.0000 72 66 060 1061713 0.4613 41.562 0.2947 0.0000 73 SS 4 90 1903467 0.3270 65 469 0 4642 0 0000 74 S7.5S3 101S818 0.4413 30 915 0.2192 0 0000 75 Sa 387 1511594 0 5567 37.104 0.2531 0 0000 76 69 390 10743430 4 S575 S2S.207 3 7306 0 0000 77 70.583 358833 0.1559 13.087 0 0928 0 0000 78 71.257 2732845 1.1373 127 212 0.9019 0.0000 79 72 317 237862 0.1033 7 948 0.05S3 0 0000 30 72.730 245290 0.1065 6.784 0.0481 0.0000 31 73.783 87523 0.0380 3.200 0.0227 0.0000 82 74.250 127009 0.0552 4 136 0.0293 0.0000 33 74.330 86115 0.0374 2,380 0.0211 0.0000 84 75.973 28738 0.0125 1.1SS 0 0083 0 0000 85 77 503 92275 0.0401 1.898 0.0135 0.0000 8β 80.060 508744 0.2210 11.408 0.0809 0 0000 37 82.200 28158 0.0122 0.371 0 0062 0 0000 38 84.293 79909 0.0347 1.534 0 0109 0.0000 39 87.500 1092447 0 4746 24.011 0.1702 0 0000 90 87.530 300045 0.1304 24.553 0.1741 0.0000 91 37.333 382295 0.1661 2S.S03 0.181S 0.0000 92 38 107 224523 0 0975 21.631 0.1534 0.0000 93 88.397 717983 0.3119 37.094 0.2S30 0.0000 94 33.010 518852 0.2254 22.242 0.1577 0.0000 95 39.237 307090 0.1334 21.551 0.1528 0.0000 96 89.473 302S3S 0.1315 19.1S8 0.1358 0 0000 97 90 007 4050733 X.7598 32S.533 2.3150 0.0000 98 91 020 376539 0.1536 16.743 0.1187 0.00187 91.427 152969 0 0665 11.423 0.0810 0.0000 100 91 S43 205346 0.0892 11.033 0.0782 0.0000 101 92.013 142333 0.0613 11.S13 0.0823 0 0000 102 92.157 263901 0.11S8 11.479 0.0814 0.0000 103 92 S33 124915 0 0543 3.073 0 0572 0.0000 104 93.083 908307 0.3946 88 37S 0.S2S6 0.0000 105 93.690 210904 0 0915 15.423 0.1093 0.0000 106 94.063 323059 0 1404 22.173 0.1572 0.0000 107 94 547 63428 0.027S 3.102 0.0220 0.0000 108 95.123 29222 0.0127 2 059 0.0146 0 0000 109 95 963 45135 0 0196 2.967 0.0210 0 0000 110 98.950 58309 0.02S3 3.S44 0 0258 0 0000 111 100 273 20525 0.0089 0 713 0.0051 0 0000 112 105 563 38299 0 01δβ 1.804 0.0128 0 0000 113 112 2β3 55710 0 0242 1 4 68 0 0104 0.0000 114 125 417 44605 0 0194 0 861 0 0051 0 0000 Sum 230177556 14105.03 0.0000

w w w w w w w w w w w w w w w w w w VP PP? P? P PP PP PV w w w w w w w w

VPvp VP PP PP PP PP

O: \ 87 \ 87623 DOOLAN 17- 200307500 The following composition analysis tests and pharmacological tests illustrate the composition and efficacy of concentrated products of Antrodia camphorata fruit solids obtained according to the cultivation method of the present invention. After analysis, the main components include triterpenoids, water-soluble polysaccharides, and chitin. Water-soluble polyacetate After testing by the Food Analysis Center, it was found that the solid body of Antrodia camphorata obtained by the cultivation method of the present invention has a high amount of water-soluble polysaccharides, and contains about 1.2 to 6.5 grams of water-soluble polysaccharides per 100 grams. Heavy metal content According to the atomic spectrometer, the heavy metal analysis of Antrodia cinnamomea solids cultivated according to the method of the present invention was performed in accordance with the atomic spectrometer in the Micro-analysis Laboratory of the Department of Atomic Science of Tsinghua University, and the results are shown as follows:

Na: 48.7 ppm Mn: 10.9 ppm Cd: ND Mg: 767 ppm Fe: 100 ppm Cr: ND A1: 41.6 ppm Zn: 16.4 ppm Ca: 605 ppm As: ND K: 0.560% Hg: ND Total Antioxidant Power (Total Antioxidant activity) The concentrated powder of Antrodia camphorata solids according to the present invention is compared with wild Antrodia camphorata powder and Ganoderma lucidum fruit body concentrated powder, and the content per gram of powder is measured, which is equivalent to milligrams of vitamin C. Source content per gram of powder / milligrams equivalent to vitamin C. Wild Antrodia powder 633 ug ascorbic acid / g Ganoderma lucidum fruit body concentrated powder 854 ug ascorbic acid / g concentrated powder of solid matter of the present invention 1180 ug ascorbic acid / g O: \ 87 \ 87623 DOOLAN -18- 200307500 Lu Hai The results show that the antioxidative power of the fruit body of the present invention and the solids of wild camphor = camphor is much higher than the toxic test Antrodia camphorata of the present invention θ a-糸 Use of old mess into acute oral toxicity test ^ 'Experimental group of rats σ taking monocular 2, GGGmg / Kg according to the stick of the present invention' control group was given purified water as a vehicle control . The experimental results showed that the mice in the experimental group did not show any abnormality or death in the mice. Therefore, the LD50 value of the acute oral toxicity test is more than 2,000 mg / Kg. test) / Antrodia camphorata according to the present invention uses the flat plate hybrid test method to carry out the Salmonella inverse mutant ㈣ (Ames test). Test g strains include five strains. Typhmmmim TA97, TA98, TA100, TA102 and Ding A1 535 five strains, five concentration analysis, respectively 312.5, 625, 125, 25 and 5 g / plate, including negative control The group and the positive control with strain specificity, and the parent group 0 were duplicated. The test results showed that the negative canine transformed colonies caused by the negative control group were all within acceptable ranges, while the positive control group also caused a significant increase in the number of reverse canine transformed colonies. All the tested concentrations of Antrodia camphorata according to the present invention, whether or not containing the rat liver enzyme (S9) metabolic system, did not cause a significant increase in the number of reverse mutant colonies of the test strains, as shown in Table 4. 〇A87 \ 8762: J DOCVLAN -19- 200307500 Table 4. Salmonella inverse mutation test subject number of experimental cases • Periodic test results Salmonella ty phimurium 5 strains each TA97 TA98 TA100 TA102 TA1535 Histidine Histidine required Tested concentration of mutant strain (Mg / plate) 312.5, 625, 1250, 2500, 5000. The plate mixed test was repeated three times. 37 ° C 1 ° C constant temperature culture for 48 ~ 72 hours. Calculate the number of mutant colonies. All test concentrations, with or without rats. The treatment of liver enzyme metabolism system (S9) did not significantly increase the number of reverse mutant colonies of the test strains. The test result is negative. The test result is negative. The test is based on the lipid peroxidation reaction of the rat brain homogenate to detect the anti-free radical and anti- Oxidation capacity. Figure 3 shows the stimulation of the rat brain homogenate with ferrous ions to induce lipid peroxidation of free radicals and increase the lipid peroxidation component (TBARS). Three-fold and eight-fold concentrated solution of Antrodia camphorata solid culture according to the present invention As its concentration increases, the results show that it can significantly inhibit the peroxidation reaction and have the ability to resist free radicals. Hepatoprotective function experiment Establish an experimental model of chronic chemical liver injury induced by tetracarbonated carbon in mice, and explore the effect of Antrodia cinnamomea at different concentration multiples on chronic liver injury in mice. Twenty-four mice without disease (ICR strain) were divided into four groups, as shown in Table 5. O: \ 87 \ 87623 DOOLAN -20- 200307500 Table 5. Test dose of Antrodia camphorata and distribution of animal groups. Carbon tetrachloride CC14 dose level. Number of rats A 0 (control group) 0 (control group) 6 B 40 % CC14 / olive oil (0.3 ml / 100 g BW) 0 (control group) 6 C as above 50 mg (triple) / kg 6 D as above 50 mg (eight times) / kg 6 on mice in groups A and B to D Subcutaneous injections of olive oil (0.1 ml / 10 g BW) and 40% carbon tetrachloride CC14 / olive oil (0.1 ml / 10 g BW) were performed twice a week, and gastric tubes were administered to mice in groups A and B. Oral saline was administered, and in groups C and D, two doses of concentrated Antrodia camphorata cultivated according to the method of the present invention were orally fed for two weeks. After the fifth week, all mice were tested for biochemical values of liver related functions by taking blood from the eye socket. Then, the mice were sacrificed, and the liver was taken and the largest right lobe liver tissue was fixed for histopathological observation, such as damaged liver cells, fat Degree of change such as degeneration, necrosis and fibrosis. Biochemical functions include blood enzymes Glutamic pyruvic transaminase (GPT) and Glutamic oxaloacetic transaminase (GOT), GPT and GOT The principle is based on the standard methods of international federal clinical chemistry.

The experimental results show that the GPT and GOT values in the plasma of mice under chronic liver injury with carbon tetrachloride increased significantly after five weeks of the experiment. The GPT value was about 850 units, which is 20-22 times the normal value. The GOT value is about 1700 units, which is 40-42 times the normal value (Figures 4 and 5). In addition, feeding three times and eight times the concentrated Antrodia camphorata of the present invention significantly inhibited the increase in GPT O: \ 87 \ 87623 EX) C \ LAN -21-200307500 and GOT values caused by carbon tetraoxide. Especially when the GpT and GOT values reach their peaks, the use of twice the concentrated dose can suppress the increase of about 37% GpT and 65% G0. As shown in Figure 6, compared to the liver tissue of normal mice, after six weeks of treatment with carbon tetrachloride, the liver tissue of the mice was significantly enlarged and the surface was gray and rough; however, it was concentrated by feeding different concentrations of Antrodia camphorata No liver swelling or gray rough surface was observed in the livers of mice. In addition, under the staining, significant accumulation of fat particles was found, liver cells near the central vein were significantly enlarged, macrophages containing iron metabolites and other inflammatory white blood cells infiltrated between liver cells, and some cells were necrotic with a nodular transformation ( nodular transfomation). No pathological changes were observed in the liver tissue of mice fed different concentrations of Antrodia camphorata (α see Figure 7). In addition, Table 6 series exemplifies the semi-quantitative analysis of liver pathological tissues in mice (N table positive # group, D table carbon tetrachloride injury group, table feeding Antrodia camphorata three-fold concentrated group, and table X8 table antarctic root feeding eight-fold concentrated group) Among them, when it can be confirmed that "Chenzhi" Chen shrinkage significantly improved pathological changes. Table 6.Semi-quantitative analysis of mouse liver pathology

The Industrial Development Research Institute accepted the solid cultivation of Antrodia cinnamomea lucidum for the present invention. Heiye cervical cancer (HeLa), gastric cancer (AGS), breast cancer (mcf_7),

O: \ 87 \ 87623 DOCVLAN -22- 200307500 Inhibition of cancer cells such as liver cancer (HepG2) and intestinal cancer (COLO 320HSR) ~, test, cell growth inhibition rate is shown in Table 7, the Antrodia camphorata solid culture ^ concentrate It has a strong inhibitory effect on tumor cells (75% -87%), of which, it has the strongest inhibitory effect on hemorrhoidal cells (MCF-7). Table 7. Test results of tumor cell inhibition by Antrodia camphorata solid culture concentrate-^ ~~-^ Cell growth inhibition rate (0/0) Cell strain type Mean (%) +

Hela 8 3 soil AGS 84 soil HepG2 75 soil MCF-7 87 soil

2.7 2.4 1.4 * The final concentration of the test sample is one tenth of the original sample concentration. In addition, five powder samples (Newi, Dynamic 3, NeW4 and New5) and ten liquid samples (20L) were used. , New2, New

520,521,522,523,524,525,526 ^ 527) products were tested in microcolumn culture tetrazolium assay for intestinal cancer cells (COL032HSR) inhibition test. The results are shown in Figure 8, which shows that the final concentration of $ 1/10 of the stock solution has at least 30% inhibition. Mouth 20L has the strongest inhibitory power, which can up to 90% of the ability to inhibit intestinal cancer cells. Analysis of sparse structure of the body Yu Jinjin v. Analysis of in vitro chromosome structural variation using Antrodia cinnamomea in accordance with the present invention

O: \ 87 \ 87623 DOOLAN -23- 200307500 cells (Chinese hamster ovary cell), and then analyze the metaphase cells, observe and calculate the frequency of abnormal chromosomes. This chromosomal structural variation analysis method can be used to evaluate the ability of Antrodia camphorata to cause damage to cell chromosomes. The analysis was performed in the following three treatments. The first was treated with Antrodia camphorata for 3 hours without rat liver activating enzyme (S 9) system. The second was treated with S9 for 3 hours. The third is to treat cells continuously for 20 hours without S9. The test concentrations and experimental results are shown in Table 8. There was no significant increase in the frequency of bow | hair chromosome variation in each test concentration of the Antrodia camphorata according to the present invention under the three treatment methods. Table 8. Analysis of in vitro chromosome structural variation. Experimental subjects. Number of experimental cases. Intake • Period-^ Experimental results. Chinese hamster ovary cells (Chinese hamster ovary cells; ATCC, repository No. CCL-61 CHO-K1). A 60-mm cell culture dish was placed at two concentrations of each type, and divided into three groups according to different processing conditions. The first and second groups were tested at concentrations of 0,312.5, 625, 1250, 2500, and 5000 ^ ml. The third group was tested at 0, 30, 100, 300, 1000, and 3000 / xg / ml After culturing according to individual conditions, select cells with a number of chromosomes 18 to 21 and spread evenly to observe '~~~~' -— The results in the three groups of treatment experiments show: according to the present invention Antrodia cinnamomea has not significantly increased the frequency of chromosomal abnormalities induced by Chinese hamster ovary cells before and after metabolic activation and at different times. Therefore, the result of this experiment is negative. Analysis of Small Nuclei in Animals According to the present invention, Antrodia camphorata causes small nuclei in the reticulocytes of mice, thereby judging whether Antrodia camphorata according to the present invention has the ability of chromosomal mutation in animals, and further provides According to the invention

O: \ 87 \ 87623 D0CJLAN -24- 200307500 Risk assessment of whether Antrodia cinnamomea is genetically toxic to humans. Twenty-five mice were divided into five groups for testing. The test methods and results are shown in Table 9. The micronucleus analysis of the mouse showed that the micronucleus analysis of the peripheral blood of the mouse according to the present invention was negative. Table IX. Small Nuclei Analysis in Animals Experimental Subjects Ingestion • Periodic test results ICR mice score 1. Solvent control group 2. High-dose group 3. Middle-dose group 4. Low-dose group 5. Positive control group Five male rats in each group 1 to 4 groups were orally administered with doses of 0,500, 1000, and 2000 mg / Kg; positive control group was administered with 1.0 mg / Kg MMC and blood was collected under conditions under a fluorescent microscope to observe reticulocytes 1. There was no significant difference in body weight between the dose group and the solvent control group. 2. Analysis of the ratio of reticulocytes: proved not to cause bone marrow toxicity in mice. 3. The proportion of small nuclei did not show a dose-response trend. There was no significant increase in the control comparison. The micronucleus analysis of the peripheral blood of mice by the invention of Antrodia camphorata was negative. Brief Description of the Drawings Figure 1 shows the appearance of fruiting bodies of Antrodia camphorata cultivated according to the cultivation method of the present invention. Figure 2 shows the high-performance phase chromatogram (HPLC) analysis and comparison of Antrodia camphorata components, including (1) the Antrodia camphorata fruit body obtained according to the cultivation method of the present invention, and (2) the Antrodia camphorata obtained by other liquid cultivation methods, and (3) represents the wild fruit of Antrodia camphorata. FIG. 3 shows that the rat brain homogenate was stimulated with ferrous ions to trigger the O: \ 87 \ 87623 DOCVLAN -25 ^ 200307500 lipid peroxidation reaction, and the cultured condensate according to the present invention expressed as a percentage of the increase in response Increased lipid peroxidation component (TBARS). The fruiting body of Antrodia camphorata grown three times and eight times thicker, and strengthens the inhibition of peroxidation. In order to inhibit peroxidation (n = 3), Figure 4 represents different concentrations of Antrodia cinnamomea that cause liver function biochemical values of carbon tetrachloride. The surface amine S-man-pyrogluconate transaminase (GPT) enzyme activity changes. Shadow 燮. # Represents a statistical difference between the normal group and the injury group (p < 001): * represents a statistical difference between the feeding group and the injury group 0 > < 0.0 ". The values are expressed as the average + standard error. Figure 5 The effect of different concentration multiples of Antrodia camphorata on carbon tetrachloride's liver function biochemical value, glutamine I, acetic acid transaminase (G0T) enzyme activity changes. # Represents a statistical difference between the normal group and the injury group ( p <0.01); * represents that there is a statistical difference between the Yishi group and the injury group (P < Gchuan. Values are expressed as mean + standard error. Figure 6 represents different concentration factors of Antrodia camphorata on liver tissue caused by carbon tetrachloride View t 4 ^ ~ ring (Table A normal group, Table B four gasification carbon injury group, Table C Gu ^ Zaozhi-^ Chen shrink group and D table dumpling Antrodia camphorata eight-fold concentrated group). Figure 7 represents different The effect of concentrated Antrodia cinnamomea on the liver tissue changes caused by carbon tetrachloride. ^, &Amp; 44 知 < θ], five weeks after feeding different concentrations of Antrodia cinnamomea in milk group, ', Staining and observing the changes of liver tissues (A table positive-thorium chloride injury group, C table was fed with Antrodia camphorata Group D table and Chi Wei out eight times concentrated food group). Effect of the rod Chi ^ Colorectal Cell extract powder (COLO 320HSR) of growth.

O: \ 87 \ 87623 DOOLAN -26,

Claims (1)

200307500 Scope of patent application: 1. A solid substance of Antrodia camphorata, which is obtained from the Institute of Food Industry Development No. CCRC 3 5 3 9 8 as the source, and cultivated by the following methods Solid solids: The method comprises culturing a mycelium of Antrodia camphorata in a space bag, removing the space bag, exposing it to the air, and performing fruit body cultivation for a period of time until a conical body is formed; The package contains 10% fiber, 10-30% starch source, 1-10% sugars, 5-1 5% grains, 0.5-2% salt filling and 0.1-1% sulfate; The fishery degree in the space bag cultivation stage is 60-80%, and the pH value is adjusted to be neutral. It is characterized in that the solid body of Antrodia camphorata has physiological activity with triterpenoids such as wild-type Antrodia camphorata. Its high-performance liquid chromatography conditions are: A. Mobile phase: 1) 30% acetonitrile / 70% 1% acetic acid ...-for 50 minutes 2) 70% acetic acid / 30% 1% acetic acid-performed 50 minutes 3) 99% acetonitrile / 1% acetic acid-... for 30 minutes B. Stationary phase: C18-chromatographic column (Merck, no. 938071) 0.5 cm Diameter X25 cm length C. Temperature: 42 ° CD · Flow rate: 1ml / min Detector: UV (254 " m), with four specific wave peaks with retention times of about 46.6, 69 · 6, 80.7, and 87.8 minutes , O: \ 87 \ 87623 DOOLAN 200307500 and its shape is conical, with a diameter of 10-20 cm, a height of 20-30 cm, and a weight of 0.3-0.5 kg. According to claim 1 of the patent scope of Antrodia camphorata fruit solids, the = empty bag is 60-70% fiber, 20% starch source, 10% five grains, 5% sugar, 1% It is composed of potassium dihydrogen phosphate and 0.5% magnesium sulfate, in which the humidity is 80%, and its ρΗ value is adjusted to be neutral. The solid substance of Antrodia cinnamomea in the first patent application scope of Genji, wherein the fibrous substance is the stem, stalk, fruit or sawdust of mushroom or plant. The solid body of Antrodia camphorata according to item 1 of the patent application, wherein the starch source is potato. The solid body of Antrodia camphorata according to item 1 of Shenyan's patent scope, wherein the grain cereal is rice bran. According to the solid body of Antrodia camphorata in item 1 of the scope of patent application, the space dagger is composed of 65% herbs, stems, stalks, fruits or wood chips, 20% potatoes, 10% rice bran, 3.5% glucose, [〇 / 〇 It consists of potassium diphosphonate and 0.5% magnesium sulfate. 7. According to the solid substance of Antrodia camphorata in item 1 of the scope of patent application, the temperature of the mycelium culture is between 5 and 32 ° C. 8. The solid body of Antrodia camphorata according to item 7 of the patent application, wherein the temperature of mycelium culture is 28 ° C. 9. The solid substance of Antrodia camphorata according to item 1 of the scope of patent application, in which the humidity of the space bag is between 60-80%. 10. The solid substance of Antrodia camphorata according to item 9 of the scope of patent application, in which the humidity of the space bag is 80%. O: \ S7 \ 87623 DOOLaN 200307500 11. 12. 13. 14. 15. 16. 17. 18. 19. According to the scope of the patent application w. Antrodia camphorata fruit solids, the air condition during the culture of the four-cell bacteria is: Contains 0.2-1% carbon dioxide. According to the scope of the patent application (1), the fruit body of Antrodia camphorata is cultivated under air circulation. : According to the application of patent No. 12, the solid body of Antrodia camphorata, the temperature during the day during the cultivation period of the fruit is between 20 and 3 generations, and the night temperature is between 8 -14 ° C. = According to the solid substance of Antrodia camphorata in item 13 of the patent application scope, the daytime temperature of the seedling cultivation is 24-26t, and the night temperature is i ° C. According to claim 14 of the scope of patent application for solids of Antrodia camphorata fruit, the temperature difference between day and night is 15. (:. According to the solid object of Antrodia cinnamomea fruit in the scope of the patent application, the humidity of the air in the fruit body culture is between 90-95%. According to the solid object of Antrodia camphora body in the application of the patent scope of No. 16, The content of carbon dioxide in the air is less than 1%. According to the solid substance of Antrodia camphorata fruit in the patent scope of the application, the mycelium soil is cultivated for 50-80 days, and the fruit body culture takes 20-50 days. According to the claimed patent, the solid substance of Antrodia cinnamomea fruit, it contains a large number of culture steps during the culture phase of the fungus carcass, including (manufactured) from the liquid bacteria I preserve the activated bacteria—small piece of agar The mycelium mass is transferred to a new sheep culture medium and cultured at a constant temperature; (2) When the bacteria grows to a strong level, the bacteria are inoculated into a space bag of sterilized fiber (such as wheat kernels or wood chips). 'Continue to culture at constant temperature; (3) When the bacteria in the space bag should be O: \ 87 \ 87623 DOCVLAN 200307500 species full of mycelium', remove the uppermost old hypha piece, and then you can inoculate a large amount into space Bao Ling. 〇 According to Shenhu Patent Scope Item 1 Antrodia camphorata @ 体 物It contains a large number of bacteria culture steps before the culture phase of bacteria and carcass, including liquid culture fermentation to mass culture bacteria, and after oblique tube culture, inoculating a large number of fermented bacteria with 5 liters of fermentation culture liquid A, Rotate and shake at 40-26 rpm for about 14 days at 24-26 ° C, continue to shake for about 14 days at 90 rpm, and inoculate into 20 liters of liquid fermentation broth for stirring and culture. The formula of the fermentation broth It is 1-3% fructose, 0.01-0.1% magnesium sulfate, 〇-ι-% yeast extract 0 05-0 · 5% potassium dihydrogen phosphate. 21. Antrodia cinnamomea fruit body according to item 20 of the scope of patent application Solid matter, wherein the formula of the fermentation broth is 2% fructose, 0.005% magnesium sulfate, 0.5% yeast extract, and 0.1% potassium dihydrogen phosphate. 22.-A product of Antrodia camphorata fruit solids, which It is characterized in that the solid substance of Antrodia camphorata according to item 1 of the scope of patent application is pulverized and concentrated to a viscous state by extraction with alcohol or water to obtain a concentrated liquid. 23. The product according to item 22 of the scope of patent application, which can be further It is made into dry powder form by drying treatment. 24. According to the scope of patent application The product of item 22, the dried powder of which can be conditioned by water and then dried to obtain a granulated product. 25. The product according to item 22 of the scope of patent application, which is a food composition. 26.丨 Item Antrodia camphorata fruit solids, which have a liver protection function. ...... 0: \ 87Λ7623 DOOLAN ^ 307500 27. 28. 29. 30. 31. 32. 33. 34. 35. According to The solid body of Antrodia camphorata which is the first item in Shenyan's patent scope, is used to cure or inhibit the growth of cancer cells. According to the 27th patent application, Antrodia cinnamomea fruit solids, the cancer cells are cervical cancer cells (HeLa), gastric cancer cells (AGS), ceremonial cancer cells (MCF_7), liver cancer cells (HepG2), and intestines. Cancer cells (C0L320HSR). The solid body of Antrodia camphorata according to item 1 of the patent application scope, which is used to inhibit the peroxidation reaction. The solid body of Antrodia camphorata according to item 1 of the patent application scope is used for anti-free radical or anti-lipid peroxidation reaction. A food composition having a hepatoprotective function, including a solid substance of Antrodia camphorata fruit body according to item 1 of the scope of patent application, or a processed product thereof. A food composition for treating or inhibiting the growth of cancer cells, comprising a solid substance of Antrodia camphorata fruit according to item 1 of Patent Application 15, or a processed product thereof. A food composition for inhibiting a peroxidation reaction, which includes a solid substance such as Antrodia cinnamomea fruit body according to item 1 of the Shen-Yue patent, or an additive thereof. A food composition for anti-free radical or anti-lipid peroxidation reaction, comprising a solid body of Antrodia camphorata as described in claim 1 of the patent scope, or a processed product thereof. -A pharmaceutical composition for hepatoprotective function, which comprises a solid substance such as an Antrodia camphorata fruit body according to item 1 of the patent application scope, or a processed product thereof: \ 87 \ 87623 DOOLAN 200307500 36 37. 38. 39. 40. 41. A long pharmaceutical composition, which is prepared from a solid species of Antrodia camphorata fruit body for treating or inhibiting cancer cell growth as described in the scope of the patent application, or its processed product. One for inhibiting the peroxidation reaction H is prepared from a solid product of antrodia angustifolia fruit body according to the preliminary application for a patent, including the item i. U-a pharmaceutical composition for use in anti-free radical or anti-lipid peroxidation reactions, which is prepared from a solid substance including camphor sylvestris, such as according to item 1 of the scope of patent application, or a processed product thereof. According to the patent claim 1, the Antrodia camphorata fruit body SI body has the same functions as natural wild Antrodia camphorata, including soothing nerves, timidity and wind, drenching blood and promoting blood circulation, detoxification in temperature, detoxification and swelling, And sedative analgesic effect, or can be used as antiseptic antidote; and has the ability to inhibit bacteria, viruses and tumor cells, with strong heart, immune regulation, anti-sympathetic effect, anti-serotonin activity; and can be used to treat gastrointestinal pain, Diarrhea, vomiting, diabetes, gout, nephritis, arthritis, inflammation, allergies, excessive urinary protein, uremia, cirrhosis, liver cancer and influenza. The solid substance of Antrodia camphorata according to item 1 of the scope of patent application, has the function of skin reconstruction and can be used as human skin or callus material. The solid substance of Antrodia camphorata according to item 39 of the patent application scope can be used as a patch for treating bedsore and skin trauma. 〇: \ 87 \ 87623 DOQLAN