KR20050079791A - Anti-helicobacter composition containing mushroom extract - Google Patents

Abstract

 The present invention relates to an anti-helicobacter composition comprising a mushroom extract. In particular, the present invention is a natural food extract does not cause toxicity and side effects to the human body, can be easily taken as a food, can be used as a substitute for synthetic antimicrobial agent, from the group consisting of roe deer mushroom, ganoderma lucidum mushroom and agaricus mushroom It provides an anti-helicobacter composition comprising an extract of the selected mushroom.

Description

ANTI-HELICOBACTER COMPOSITION CONTAINING MUSHROOM EXTRACT}

[TECHNICAL FIELD OF THE INVENTION]

The present invention relates to an anti-helicobacter composition comprising a mushroom extract, and more particularly, does not cause toxicity and side effects to the human body, and can be easily taken as food, and can be used as an alternative to synthetic antimicrobial agents. It is about.

[Private Technology]

Helicobacter pylori is a Gram-negative bacterium that was first isolated and identified in human gastric mucosa by Warren and Marshall in 1983. It was originally named Campylo bacter pyloridis, but it was named Helicobacter pylori, citing the shape of the body and the name of the pylorus that inhabit the stomach.

Helicobacter pylori is also found in the mucosa of the esophagus and duodenum, but most of it proliferates in contact with epithelial cells in the stomach, especially about 0.5 mm thick gastric mucus. Helicobacter pylori is known to be associated with the development of gastritis, gastric ulcer, peptic ulcer, duodenal ulcer and gastric cancer. Once infected with Helicobacter pylori, the infection lasts for decades and is rarely eliminated naturally. Helicobacter Philly is a major cause of chronic gastritis.

As a treatment for gastritis, gastric ulcer and duodenal ulcer, H2 blockers that suppress gastric acid secretion are mainstream. Such agents have a high therapeutic effect, but have a high relapse rate. This is because the above agents do not directly act on Helicobacter Philophyll, but mainly on gastric acid secretion inhibition. For this reason, a lot of research has recently been conducted on preparations that directly act on Helicobacter pylori, which causes gastritis, gastric ulcer and duodenal ulcer.

In the above studies, a number of lactic acid bacteria having antimicrobial activity in Helicobacter pylori (Korean Patent Publication Nos. 2003-82509, 2003-77895, 2002-46168, 2002-46167, 2002-46166) And Bacillus sp. Strains (Korean Patent Laid-Open Publication Nos. 2003-75003 and 2003-75716) have been identified. In addition, antibodies prepared by using a virulence factor of Helicobacter pylori as an antigen have been developed, and bismuth and Therapies have been developed using triple therapies combining amoxicillin or tetracycline with metronidazole, and quadruple therapy with a combination of bismuth, omeprazole, tetracycline and netronicazole. . However, in the treatment method using antibiotics, the emergence of resistant strains and the problem of recurrence after drug treatment still remain, the development of a treatment method that can solve this situation is required.

Therefore, an object of the present invention is to provide a natural plant extract having an antimicrobial activity against Helicobacter pylori.

It is another object of the present invention to provide an anti-helicobacter composition that is harmless to a human body and can be taken as a food.

In order to achieve the above object, the present invention provides an anti-helicobacter composition comprising an extract of a mushroom selected from the group consisting of roe deer mushroom, ganoderma lucidum mushroom and agaricus mushroom.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention, while searching for natural foods exhibiting antimicrobial activity against Helicobacter pillori, confirmed that locust mushroom, Ganoderma lucidum mushroom and Agaricus mushroom had excellent anti-Helicobacter activity, and completed the present invention.

The present invention relates to an anti-Helicobacter composition comprising an extract of a mushroom selected from the group consisting of roe deer mushroom, ganoderma lucidum mushroom and agaricus mushroom as an active ingredient.

The roe deer mushroom, ganoderma lucidum mushroom and agaricus mushroom of the present invention may be collected from ordinary nature or artificially cultured. The artificial culture is by a conventional culture method, can be practiced by those skilled in the art.

In one embodiment, the roe deer mushroom may be cultured in jinjin wormwood. The deer worm mushroom cultured in Injin mugwort may be obtained by incubating the mushroom spawn in a common mushroom liquid medium and inoculating and incubating it in Injin mugwort, which is carried out by the method described in Korean Laid-Open Patent Publication No. 2002-72878. Can be obtained. Briefly described as follows. However, specific culture methods and culture conditions can be easily changed by those skilled in the art.

Roe deer beetle cultured in Injin mugwort

(a) obtaining a seed of the roe deer mushroom;

(b) preparing a solid medium comprising phosphorus mugwort; And

(C) it can be prepared by the method comprising the step of inoculating and incubating the scavenger mushroom seedling in the solid medium to obtain a mycelium.

It may also further comprise the step of obtaining a fruiting body by further culturing the mycelium.

In the step (a), the seedling of the roe deer fungus is inoculated into the cultivation of the roe deer fungi, and cultured for 5 to 8 days at 23 to 26 ℃, pH 5 to 6, stirring speed 100 to 140 rpm It can be obtained by. At this time, the culture temperature, the pH of the medium, the stirring speed and the incubation period set in the above range is the maximum to obtain the worm mushroom mycelium, the liquid culture medium for the spawn YMPG, MCM, PDA or Soybean meal can be used.

In the step (b), the solid medium is prepared by sterilizing after adding water to the jinjinjin. Water may be added in a weight ratio of 1: 1 to 10 with respect to phosphorus mugwort, but is not limited thereto. The sterilization method can be carried out by a known method, preferably high temperature sterilization method.

In the step (c), seed spawn obtained in step (a) is inoculated in a solid medium at 0.1 to 30% by weight, and cultured at a culture temperature of 23 to 26 ° C. and a culture period of 40 to 50 days. Mycelia can be obtained by the primary growth as described above, the mycelium can be further grown in culture for 15 to 20 ℃, the secondary growth for 10-15 days in the culture period to further culture the fruiting body.

The method of cultivating roe deer mushroom in the above-mentioned jinjin wormwood is only one example and is not limited to the above.

Ganoderma lucidum and Agaricus mushrooms of the present invention may also be cultured in Injin mugwort by the above method.

The mushroom extract according to the present invention may be prepared by extracting any one of mycelium, fruiting bodies, liquid cultures and mixtures thereof of mushrooms with water or an organic solvent. For example, the mushroom extract may be prepared by obtaining or fractionating the supernatant by immersing the mushroom in at least one extraction solvent, and then may be powdered through spray drying or lyophilization. As the organic solvent, alcohols of 1 to 5 carbon atoms, hexane, chloroform or ethyl acetate may be used, and as alcohols, ethanol, methanol, butanol, propanol or isopropanol may be used. The alcohol may be used as a 100% stock solution, but 90 to 50% alcohol may be used. However, the extraction solvent is not limited to the solvents described above, and any known solvent can be used. The preferred extraction method is to immerse the mushroom in an extract solvent of 1: 1 to 20 by weight, and then filter it, and then depressurize, concentrate and spray-dry or lyophilize.

Each of the mushroom extracts according to the present invention is extractable from edible mushrooms, and therefore, is not only toxic to the human body but also easily taken as food. In addition, the mushroom extract according to the present invention exhibits significantly superior antimicrobial activity compared to metronidazole and tetracycline which are used as therapeutic agents for Helicobacter pylori.

The anti-helicobacter composition of the present invention includes one or more mushroom extracts as an active ingredient, and may further include a carrier or excipient pharmacologically usable according to the formulation and the method of use. In this case, the content of the active ingredient in the anti-helicobacter composition may be 0.001 to 99% by weight. If the amount of the active ingredient in the composition is less than 0.001% by weight may require a large amount of administration for effective efficacy, if the amount exceeds 99% by weight may be constant compared to the amount may be uneconomical. However, it is preferable to properly adjust the content of the active ingredient according to the method of use and formulation of the anti-helicobacter composition.

The pharmacologically acceptable carrier or excipient may use a conventional material depending on the dosage form, and when formulated, fillers, extenders, wetting agents, disintegrating agents or surfactants may be used. Representative diluents or excipients include water, dextrin, calcium carbonate, lactose, propylene glycol, liquid paratin and physiological saline.

The anti-Helicobacter composition may be administered orally or parenterally, and its formulation may vary depending on the method of use, and thus is not limited thereto. Examples of formulations include PLASTERS, GRANULES, LOTIONS, POWDERS, SYRUPS, LIQUIDS AND SOLUTIONS, AEROSOLS, OINTMENTS, FLOWERS FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, INFUSIONS, TABLETS, INJECTIONS, CAPSULES and PILLS.

The dosage or amount of the antihelicobacter composition may be determined in consideration of the form of administration, the age, sex, condition of the patient, the absorbency of the active ingredient in the body, the inactivation rate, and the concomitant drug, for example, based on a daily active ingredient. When used, it can be used at 0.001 mg / kg body weight to 500 mg / kg body weight.

The anti-helicobacter composition according to the present invention can be used as a food, a food additive, a beverage or a medicament, and can be easily utilized as a functional food or a beverage.

Hereinafter, examples of the present invention will be described. The following examples are only for illustrating the present invention and the present invention is not limited to the following examples.

Example 1 Preparation of Extract of Roebuck Mushroom

YMPG (KH ₂ PO ₄ 2.0 g / L, MgSO ₄ · 7H ₂ O 1.0 g / L, glucose 10.0 g / L, thiamine-HCl 1.0 g / L, adjusted to pH 5.5 for Hericium erinaceus fungi DL-asparagine 1.0 g / L, peptone 2.0 g / L, malt extract 10.0 g / L, yeast extract 2.0 g / L, agar 20.0 g / L) or MCM (K ₂ HPO ₄ 1.0 g / L, KH ₂ PO ₄ 0.46 g / L, MgSO ₄ .7H ₂ O 0.5 g / L, glucose 20.0 g / L, peptone 2.0 g / L, yeast extract 2.0 g / L, agar 20.0 g / L) The inoculum was incubated by shaking culture for 7 days at 120 rpm with agitation speed.

Water corresponding to twice the total weight of the phosphorus mugwort was added to the mugwort, which was sterilized at 120 ° C. for 2-4 hours to prepare a solid medium.

The inoculum was inoculated at 10% by weight based on the weight of the solid medium, and mycelia were first grown at 25 ° C. for 40 to 50 days. Thereafter, fruiting bodies were grown by performing secondary growth at a culture temperature of 15 to 20 ° C. for 10 to 15 days.

The mycelium obtained above was dried at room temperature for 24 hours, and then 3 L of 30% ethanol was added to 145 g of the dried product and extracted at room temperature for 2 weeks. The ethanol extract was filtered to give an ethanol extract, which was concentrated at 80 ° C. and then lyophilized to give 20.5 g of dried product. Hereinafter, the ethanol extract, that is, lyophilisate is named "Arinacol".

Example 2: Roebuck Mushroom Extract

After crushing 75 g of roe deer mushroom dried product, 3 L of 30% ethanol was added and extracted at room temperature for 2 weeks. The extract was filtered to give the filtrate, which was concentrated at 80 ° C. and lyophilized to give 41.9 g of dried product.

Example 3: Ganoderma lucidum mushroom extract

After crushing the dried ganoderma lucidum 145 g finely, 3 L of 30% ethanol was added and extracted at room temperature for 2 weeks. The extract was filtered to give the filtrate, which was concentrated at 80 ° C. and lyophilized to give 4.3 g of dry matter. The above procedure was repeated to give a total of 16.4 g.

Example 4: Agaricus Mushroom Extract

145 g of agaricus dry matter was finely crushed, and 3 L of 30% ethanol was added thereto, followed by extraction at room temperature for 2 weeks. The extract was filtered to give the filtrate, which was concentrated at 80 ° C. and lyophilized to give 78.3 g of dry matter.

Comparative Example 1-2

Metronidazole and Tetracycline were purchased from Sigma, respectively, and used as Comparative Examples 1 and 2.

Experimental Example: Antihelicobacter Activity

Screening of antimicrobial activity against Helicobacter pylori was performed by the following method.

Helicobacter Philoly was incubated in Brucella agar medium for 48 hours, and then inoculated in Brucella liquid medium and suspended in 450 nm for absorbance at 450 nm. 200 ml of the suspension is taken, plated in a Brucella agar medium, and then placed on a sterile disk (Bacto Concentration Disk), and the mushroom extracts of Examples 1 to 4 and the samples of Comparative Examples 1 to 2 are dissolved in DMSO. Each was titrated to 0.05 mg. Then, after incubating at 37 ° C. for 72 hours under 10% CO ₂ conditions, the size of the growth zone formed around the disc was measured. The results are shown in Table 1 below.

sample Growth low ring (mm) Example 1 20 Example 2 <10 Example 3 <10 Example 4 <10 Comparative Example 1 31 Comparative Example 2 53

In Table 1, the mushroom extracts of Examples 1 to 4 showed very good anti-helicobacter activity compared to the metronidazole and tetracycline of the comparative example.

Example 5: Pharmaceutical Preparation

5-1. Powder

1 g of lactose was mixed with 2 g of the extract of the Roestock fungus cultured in Injin mugwort of Example 1, and filled with an airtight cloth to prepare a powder.

5-2. refine

Tablets were prepared by mixing 100 mg of the extract of the Roestock fungi cultured in Injingumi of Example 1, 100 mg of corn starch, 100 mg of lactose, and 2 mg of magnesium stearate, followed by compression according to a conventional tablet preparation method.

5-3. Capsule

A capsule was prepared by mixing 100 mg of the extract of the scavengerae mushroom cultured in Injin mugwort of Example 1, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate, and then filling the gelatine capsule.

5-4. Injection

Injectable extract was prepared by adding a suitable amount of distilled water for injection to 100 mg of the extract of the Roestock fungus cultured in Injinguchi of Example 1, adjusting the pH to about 7.5, and then filling and sterilizing the 2 ml ampoule.

Example 6: Functional Food Preparation

6-1. Wire

Brown rice, barley, glutinous rice, and yulmu were alphatized by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh using a grinder. Black beans, black sesame seeds and perilla were each steamed and dried in a known manner, then roasted and ground to prepare a powder having a particle size of 60 mesh.

Then, 30% by weight brown rice, 15% by weight of barley, 20% by weight of barley, 9% by weight of glutinous rice, 7% by weight of perilla, 8% by weight of black soybeans, 7% by weight of black sesame seeds, snail mushroom incubated in Injin mugwort of Example 1 3% by weight of the extract, 0.5% by weight of Ganoderma lucidum and 0.5% by weight of turmeric was prepared to prepare a wire.

6-2. beverage

0.26% by weight of honey, 0.0002% by weight of thioctoamide, 0.0004% by weight of nicotinic acid, 0.0001% by weight of riboflavin sodium hydrochloride, 0.0001% by weight of pyridoxine hydrochloride, 0.001% by weight of inositol, 0.002% by weight of orthoic acid, 98.7362% by weight of water and examples 1% by weight of the extract of the Roer mushroom was cultured in the Injin mugwort of 1 to prepare a healthy drink in a conventional manner.

As described above, the extract of the mushroom selected from the group consisting of Roebuck mushroom, ganoderma lucidum mushroom and agaricus mushroom of the present invention not only exhibits significant anti-helicobacter pilori activity, but also is a natural food extract and thus does not cause toxicity and side effects to the human body. It can be easily taken as a food. Therefore, the mushroom extract of the present invention can be used as a substitute for existing synthetic antimicrobial agents.

Claims (6)

An anti-helicobacter composition comprising an extract of a mushroom selected from the group consisting of roe deer mushroom, ganoderma lucidum mushroom and agaricus mushroom as an active ingredient. The composition of claim 1, wherein the extract is extracted with water, alcohol having 1 to 5 carbon atoms, hexane, ethyl acetate or chloroform. The composition of claim 1, wherein the mushroom extract is obtained by extracting mushroom mycelium and / or fruiting bodies with ethanol. The composition of claim 1, wherein the roe deer mushroom is cultured in jinsengui. The method according to claim 4, wherein the roe deer mushroom (a) inoculating the scab mushroom into a liquid culture medium for spawn, and shaking it for 5 to 8 days at 23 to 26 ° C., pH 5 to 6, and a stirring speed of 100 to 140 rpm to obtain a spawn seed; (b) adding solid water to phosphorus mugwort in a ratio of 1 to 1 to 10 and then sterilizing to prepare a solid medium; And (c) inoculating the seed medium obtained in step (a) at a solid medium of 0.1 to 30% by weight, and incubating the same at a culture temperature of 23 to 26 ° C. and a culture period of 40 to 50 days to obtain a mycelia; The composition is cultured in a method comprising.  The composition of claim 1, wherein the composition is a food, food additive, beverage or medicament.