TNSN08356A1 - A METHOD FOR " in embryo" transformation with agrobacterium of wheat and production of transgenic fertile plans over-expressing the AISAP gene, isolated from the halophyte grass " A littoralis", for improvement of abiotic stress tolerance - Google Patents
A METHOD FOR " in embryo" transformation with agrobacterium of wheat and production of transgenic fertile plans over-expressing the AISAP gene, isolated from the halophyte grass " A littoralis", for improvement of abiotic stress toleranceInfo
- Publication number
- TNSN08356A1 TNSN08356A1 TNP2008000356A TNSN08356A TNSN08356A1 TN SN08356 A1 TNSN08356 A1 TN SN08356A1 TN P2008000356 A TNP2008000356 A TN P2008000356A TN SN08356 A TNSN08356 A TN SN08356A TN SN08356 A1 TNSN08356 A1 TN SN08356A1
- Authority
- TN
- Tunisia
- Prior art keywords
- agrobacterium
- seeds
- gene
- aisap
- halophyte
- Prior art date
Links
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a plant transformation method through treating germinating cereal seeds with Agrobacterium. Seeds of wheat at the early germinating stage were used as receptors and the binary pCAMBIA-1390W plasmid, harboured in a disarmed Agrobacterium strain EHA105, carrying an inserted T-DNA fragment was used as a gene donor. The T-DNA is composed with the hygromycin phosphotransferase gene (denoted bpt, hph or aph IV confers resistance to the hygromycin antibiotic) under the control of 35S CaMV promoter and the AISAP gene (coding for a stress associated zinc finger protein), isolated from the halophyte grass Aeluropus littoralis, under the control of the Act 1 promoter. The recombinant Agrobacterium strain was injected in the embryos of imbibed seeds and during the two days of co-cultivation the T-DNA fragment was transferred to the germ line. Then the seedlings from the injected seeds were incubated in a solution containing cefotaxim to remove Agrobacterium and finally were grown in green house during a whole cycle. ln some of the produced seeds, PCR amplification verified the integration of the alien AISAP and hpt genes into the receptor plant and can inherit to the next generation.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TNP2008000356A TNSN08356A1 (en) | 2008-09-17 | 2008-09-17 | A METHOD FOR " in embryo" transformation with agrobacterium of wheat and production of transgenic fertile plans over-expressing the AISAP gene, isolated from the halophyte grass " A littoralis", for improvement of abiotic stress tolerance |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TNP2008000356A TNSN08356A1 (en) | 2008-09-17 | 2008-09-17 | A METHOD FOR " in embryo" transformation with agrobacterium of wheat and production of transgenic fertile plans over-expressing the AISAP gene, isolated from the halophyte grass " A littoralis", for improvement of abiotic stress tolerance |
Publications (1)
Publication Number | Publication Date |
---|---|
TNSN08356A1 true TNSN08356A1 (en) | 2009-12-29 |
Family
ID=59799023
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TNP2008000356A TNSN08356A1 (en) | 2008-09-17 | 2008-09-17 | A METHOD FOR " in embryo" transformation with agrobacterium of wheat and production of transgenic fertile plans over-expressing the AISAP gene, isolated from the halophyte grass " A littoralis", for improvement of abiotic stress tolerance |
Country Status (1)
Country | Link |
---|---|
TN (1) | TNSN08356A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109652438A (en) * | 2018-12-25 | 2019-04-19 | 河南农业大学 | A kind of Wheat genetic transformation method based on wheat seed embryo injection Agrobacterium |
-
2008
- 2008-09-17 TN TNP2008000356A patent/TNSN08356A1/en unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109652438A (en) * | 2018-12-25 | 2019-04-19 | 河南农业大学 | A kind of Wheat genetic transformation method based on wheat seed embryo injection Agrobacterium |
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