TH9580B - Chinese recombinant with code for human alpha eater and vector characterizing it, etc. - Google Patents

Abstract

Here we reveal a Chinese recombinant that codes the human alpha interpiron for its efficient characterization in yeast. The sequence of nucleotides shown in Fig. 1A is a vector characterizing the Chinese. Vector-transfected yeast cells exhibit this trait. The method for producing human alpha-interpiron using the said transformed yeast. and a method for purifying human alpha-interpiron with high yields.

Claims (4)

1.Chinese recombinant alpha interpian of people with the following nucleotide sequence 30 ATG TGT GAT CTG CCT CAA ACT CAC AGC CTG 60 GGT TCT AGA AGA ACC TTG ATG TTG CTA GCT 90 CAA ATG AGA AAG ATA AGC TTG TTC TCC TGC 120 TTG AAG GAC AGA CAT GAC TTT GGA TTT CCC 150 CAG GAG GAG TTT GGC AAC CAG TTC CAA AAG 180 GCT GAA ACC ATC CCT GTC CTC CAT GAG ATG 210 ATC CAG CAG ATC TTC AAT CTC TTC AGC ACA 240 AAG GAC TCA TCT GCT GCT TGG GAT GAG ACC 270 CTC CTA GAC AAA TTC TAC ACT GAG CTC TAG 300 CAG CAA CTG AAT GAC CTG GAA GCC TGT GTG 330 ATA CAG GGG GTG GGG GTG ACA GAG ACT CCC 360 CTG ATG AAG GAG GAC TCC ATT CTG GCT GTG 390 AGG AAA TAC TTC CAA AGA ATC ACT CTC TAT 420 CTG AAA GAG AAG AAA TAC AGC CCT TGT GCC 450 TGG GAG GTT GTC AGA GCA GAA ATC ATG AGA 480 TCC TTT TCT TTG TCA ACA AAC TTG CAA GAA .AGT TTA AGA AGT AAG GAA TAA TAG 2. A trait vector consisting of a Chinese recombinant alpha interpian of a person according to claim 1, with China in a position that can characterize in Yeast cells 3. Vector of claim 2 which is (Chemical formula) 4. Vector of claim 2 which is (Chemical formula) 5. Yeast cell transformed by vector characterizing claim 2 6. Transfusion yeast cell of claim 5, Saccaromycescerevisiae DC04 (Chemical formula) 7. Transfound yeast cell of claim 5, Saccaromycescerevisiae DC04 (Chemical formula) 8. Process for producing alpha inter Persons with a yeast cell culture of either claim 5,6 or 7 9. Human alpha interpian produced by the process of claim 8 1 0. Purification process of human alpha interpian produced in yeast cells, consisting of a method for replacing human alpha interpian, which is not shown. The effect is in a demonstrable form consisting of steps of (A) dissolving human alpha interpiron produced in yeast in a solution containing Reducing agent and regulating human alpha interpian reduction under conditions for it to reorganize (B) through a solution containing human alpha interpian to rearrangement into chromatography. The raphylaxis anions to separate the reorganized human alpha interpianons (C) through human alpha interpianons into cation exchange chromatography. To isolate human-neutralized alpha interpirons from active human alpha interpirons (D), inactivated human alpha interpirons with oxidizing redoxidants. Xthylol and reducing thiol group to turn it into a 1 1. Procedure of claim 10, where the redox substance has a reducing thiol approximately 1 to 10 mM and oxidized thiol approximately 0.1 to 1 mM. 2. Process of claim 10 where redox consists of cysteine and cystine 1. 3.Procedure of claim 10, where such redox compounds contain oxidized glutathione and reducing glutathione 1. 4.Polypeptide Alpha Interpian of human purified by the process of claim 10.