TH3872B - Elastase I enzyme preparations from human pancreas. - Google Patents

Abstract

Preparation of elastase I from a human pancreas can be performed by the technique of engineering varieties.

Claims (8)

1. A process for producing compounds that can act like human pancreatic elastase. The process consists of a process of cultivating a house that has been transformed sequentially. DNA coded for compounds capable of acting like elastase I from the human pancreas. The cultured conditions were suitable for the transcription of DNA sequences and extracted compounds that act like elastase I from the human pancreas. 2. Process according to claim 1, wherein the isolated compound, either the amine sequence of elastase I from the human pancreas, or any protein As effective as elastase I from a person's pancreas. The amino acid sequence of elastase I from human pancreas is as follows: (N) - Val Val Gly Gly Thr Glu Ala Gly Arg Asn Ser Trp Pro Ser Gln Ile Ser Leu Gln Tyr Arg Ser Gly Gly Ser Trp Tyr His Thr Cys Gly Gly Thr Leu Ile Arg Gln Asn Trp Val Met Thr Ala Ala His Cys Val Asp Tyr Gln Lys Thr Phe Arg Val Val Val Ala Gly Asp His Asn Leu Ser Gln Asn Asp Gly Thr Glu Gln Tyr Val Ser Val Gln Lys Ile Val Val His Pro Tys Trp Asn Ser Asp Asn Val Ala Ala Gly Tyr Asp Ile Ala Leu Leu Arg Leu Ala Gln Ser Val Thr Leu Asn Ser Tyr Val Gln Leu Gly Val Leu Pro Gln Gln Gly Ala Ile Leu Ala Asn Asn Ser Pro Cys Tys Ile Thr Gly Trp Gly Lys Thr Lys Thr Asn Gly Gln Leu Ala Gln Thr Leu Gln Gln Ala Tyr Leu Pro Ser Val Asp Tyr Ala Ile Cys Ser Ser Ser Ser Tyr Trp Gly Ser Thr Val Lys Asn Thr Met Val Cys Ala Gly Gly Asp Gly Val Arg Ser Gly Cys Gln Gly Asp Ser Gly Gly Pro Leu His Cys Leu Val Asn Gly Lys Tyr Ser Leu His Gly Val Thr Ser Phe Val Ser Ser Arg Gly Cys Asn Val Ser Arg Lys Pro Thr Val Phe Thr Arg Val Ser Tyr Ile Ser Trp Ile Asn Asn Val I le Ala Ser Asn? (C) where (N) instead of (1) Met (2) Thr Gln Asp Leu Pro Glu Thr Asn Ala Arg or (3) any peptide of Part (2) by It is conditional that an Arg segment must be present at end C. 3. The process according to claim 1, wherein the isolated compound is proelastase. Pre-elastase or protein binding 4. The process according to claim 1 by host Shelter is yeast bacteria. Or mammalian cells 5.Processes for producing elastase I from human pancreas. The procedure consists of the following steps: (1) Freshly inserted DNA for elastase into the transcription carrier (2) recombinant DNA into host (3) cultured Jebon in the resulting state. Transcription of the DNA sequence and (4) separating compounds including elastase. 6. The process mentioned in claim 5 where the DNA in step (1) is sequenced as follows (5?) - GTA GTC GGA GGG ACT GAG GCC GGG AGG AAT TCC TGG CCC TCT CAG ATT TCC CTC CAG CGG. TCT GGA GGT TCC TGG TAT CAC ACC TGT GGA GGG ACC CTT ATC AGA AAC TGG GTG ATG ACA GCT GCT CAC TGC GTG GAT TAC CAG AAG ACT TTC CGC GTG GTG GCT GGA GAC CAT AAC CTG AGC CAG AAT GAT GGC ACT GAG CAG TAC GTG AGT GTG CAG AAG ATG GTG GTG CAT CCA TAC TGG AAC AGC GAT AAC GTG GCT GCC GGC TAT GAC ATC GCC CTG CTG CTG CGC CTG GCC CAG AGC GTT ACC CTC AAT AGC TAT GTC CAG CTG GGT GTT CTG CCC CAG GAG GGA GCC ATC CTG CTG GCT AAC AAC AGT CCC TGC TAC ATC ACA GGC TGG GGC AAG ACC AAG ACC AAT GGG CAG CTG GCC CAG ACC CTG CTG CAG CAG GCT TAC CTG CCC TCT GTG GAC TAT GCC ATC TGC TCC AGC TCC TCC TAC TGG GGC TCC ACT GTG AAG AAC ACC ATG GTG TGT GCT GGT GGA GAT GGA GTT CGC TCT GGA TGT CAG GGT GAC TCT GGG GGC CCC CTC CAT TGC TTG GTG AAT GGC AAG TAT TCT CTC CAT GGA GTG ACC AGC TTT GTG TCC AGC CGG GGC TGT AAT A GTC CCTCC AG ACA GTC TTC ACC CGG GTC TCT GC T TAC ATC TCC TGG ATA AAT AAT GTC ATC GCC TCC AAC X- (3?) Where X is the stop code (TAA, TGA or TAG), AATG or some or all of the sequence (5?) - ACC CAG GAC CTT. CCG GAA ACC AAT GCC CGC- (3?); Or a base sequence with a similar function 7. Procedures according to claim 5, wherein the isolated compounds, including the amino acid sequences of elastase I from human pancreas or any protein As effective as elastase from human pancreas 8. Process according to claim 5, where the host is yeast bacteria. Or mammalian cells