TH82C3 - Leptospirosis reagent set Preparation process And techniques of latex latinization - Google Patents

Abstract

It revealed the selection of chirovars of leptospira to be used as antigens and the production process of the assay used in the diagnosis. Leptospirosis That gives quick results, is highly accurate, convenient and easy to test And it is cheap to use in the general laboratory. And can be used in the field

Claims (1)

Disclaimer (all) which will not appear on the advertisement page: 1 Antigen obtained from the selection of The Shirovar-type leptopira is believed to detect most of the Shirovars, namely, xiro, pyrogenase, Bratislava and other Shirovars 5 from laboratory analysis to collect data. Outbreaks are selectively used as antigens of the common Shirovar type leptospira. To develop a trial kit 2. Reagent using antigens according to claim 1, which has a method of preparation for step 1, leptospira preparation for preparation of antigen. And preparation of latex tablets. Step 2: Preparation of test reagents The details of each step are as follows: Step 1: Leptospira preparation for entgenology and latex tablet preparation. Leptospira culture methods and various reagents 1. Preparation of liquid culture medium for use for leptospira culture consisting of neopeptone medium weighing 0.4 g. Mix with 0.2 g of crotone sodium salt, add 200 ml of distilled water, boil in boiling water to mix well, adjust pH at 7.4, steam in 15 lb autoclave at 121 ° C. For 15 minutes, the rabbit serum was prepared by warming it in warm water (water bath) at a temperature of 56 ° C for 30 minutes and filtered through 0.45 micron filter paper. Prepare a medium to cultivate leptose. Fungus by mixing the serum of the warm rabbit. Mix with liquid culture medium at a ratio of 1: 10 parts by aseptic technique, bring the mixed culture medium and warm it to 65 ° C for 1 hour and repeat for 2 days in a row. Liquid type for culture Stored at room temperature for 1-2 weeks 1. Mix 1 part latex with borat buffer silai solution. 5 parts are blended in a blender at a speed of 5,000 rpm at a temperature of 4 degrees Celsius for 15 minutes, vacuuming the clear part by pipetting the latex sludge to spin and rinse. Repeat 3 times, after 3 times, dissolve the Latex sludge mixture. The 5 parts borate buffer silicate solution was stored at 4 ° C for 6 months. The preparation of the test kit by coating the Leptose Spill antigen on the latex tablet was performed as follows. Mix 1 part of the prepared antigen with 1 part of the prepared lattex, shake well for 1 hour and store it at 4 ° C so that the antigen is coated on the lattex seed. The next day, it was blended with a blender at a speed of 5,000 rpm at 4 degrees Celsius for 15 minutes. 5 parts of the sludge was added to the blocking buffer. Shake it with a shaker for 2 hours at a temperature of 4 ° C. Blocking buffer is repeated 3 times with the same blender. Will cause the antigen to believe the leptospira is firmly coated on the latex beads In the final step, the sediment was dissolved with 17 part phosphate buffer, the test solution prepared at 4 ° C, and stored for 6 months.