TH77374B - Method for producing the flu virus - Google Patents

Abstract

DC60 (07/01/16) This invention relates to a method for producing influenza virus in which a. influenza virus is given to hens b. eggs from immunized hens c. stimulates embryogenesis. d. The embryo is infected. By inoculating the influenza virus into allantoic cavity e. infected embryo eggs. cured under temperature conditions and proper humidity to allow viral replication; and f. collection of viral allantoic fluid. Edit Invention Summary 07/01/16 This invention relates to a method for producing the influenza virus where: a. the influenza virus is given to the hens b. the eggs from the immunized hens are collected c. embryo is born d. embryo egg is infected By injecting the influenza virus into allantoic cavity e. infected embryo eggs. cured under temperature conditions and proper humidity to allow viral replication; and f. collection of viral allantoic fluid. ----------------------------------------------- This invention Relevant to methods for producing influenza virus where: a. influenza virus is given to hens b. eggs taken from immunized hens c. embryogenesis is stimulated d. embryo eggs are fertilized infect By injecting the influenza virus into allantoic cavity e. infected embryo eggs. cured under temperature conditions and proper humidity To allow viral proliferation and f. collection of viral allantoic fluid: patent drug

Claims (8)

Disclaimer (all) which will not appear on the advertisement page. : ------ 12/02/2019 ------ (OCR) Page 1 of 3 pages Disclaimer 1. Method for producing influenza virus where: a. Flu vaccine is given to hens b . Eggs from immunosuppressed hens c. Stimulates embryo processes d. Embryo eggs are then infected by inoculating the influenza virus into the allantoic cavity. (allantoic cavity) e. The infected embryo eggs were incubated under temperature conditions. And f. Storage of allantoic fluids containing the virus 2. Method according to claim 1, where the vaccine protects the hen from avian influenza. 3. Pursuant to claim 1 or 2, where the flu vaccine contains influenza virus haemacutinin as a protein and / or the genes coding this protein in the vaccine component. 4. Method according to the request for the right to claim rights 1 to 3, any one Where the components of the flu vaccine contain all the flu viruses that have been depleted; 5. Method according to one of the claims 1 to 4 The flu vaccine component contains all flu virus products. 6. Method according to the claim number 1 to 5 any one. Where the component of the vaccine contains adjuvants. 7. Method according to claiming rights 1 to 3, any one The flu vaccine component contains a weakened flu virus. 8. Any method of claiming 1 to 3. Where the flu vaccine is composed of a vector composed of a gene that encodes the hema glutinin code of the influenza virus 9. The method by which Claim 8 is applied, where the vectori is masked. Assuming 1 0. Method as requested for claim 8 or 9, where the vector is also composed of genes that encode the neraminidase of influenza virus. Page 2 of the number 3 Page 1 1 The method used for claiming rights in any of the 8th to 10th claims Where the composition of the vaccine contains adjuvants as well 1. 2. Method according to the claim number 3 to 11 any one Where hema glutinin, the flu virus, is in the form of a protein and / or coded gene. This protein is contained in the vaccine component of the hen. The flu virus hemagglutinin used to cause allantoiccaví to hit in embryo eggs from immunocompromised hens had different subtypes. 1 3. Method of claiming rights in Claims 3 to 11, any one of Where hema glutinin of the influenza virus is in the form of a protein and / or code-coded gene. And the hemagglutinin of influenza virus used to cause callantoiccavity in embryo eggs from infected immunosuppressed hens has the same chaptiape1 4. Method according to the request for claiming rights in any of the 3rd to 11 Where hema glutinin of the influenza virus is in the form of a protein and / or code-coded gene. And the hemagglutinin of the influenza virus used to induce Callantoic Cavity in embryo eggs from immunized and infected hens is the same. Holds the right to any of the rights 4, 6 or 7 The flu virus contained in the vaccine for the hens is the same as the influenza virus used to infect the young callantoiccavity in embryo eggs from immunized hens. 6. Any method sought by claim 12 to 15 where the flu virus haemagglutinin is in the form of a protein and / or the genes that code this protein contained in the composition. Of the vaccine given to the hen And the hemagglutinin of influenza virus used to cause callantoiccaví to hit in embryo eggs from infected immunosuppressive hens contained subtypi H5, H6, H7 or H9 1 7. Means as claimed in claim 16, where influenza virus hemagglutinin is in the form of a protein and / or coded gene. This protein is contained in the composition of the vaccine used to Stimulate the hen's immune system against the flu. And hemagglutinin of influenza virus used to cause callantoiccaví to hit in embryo eggs from infected hens with subtypes H5 or H7 1 8. Method As requested in any of Claims 1 to 17 which includes one additional step Of the viral purification process Page 3 of the number 3 Page 1 9. Method according to the right of claim 1 to 18 any of the above. Which includes one additional step Of the process of eliminating the virus. 2 0. Flu vaccine consisting of influenza virus obtained by using one of the methods requested in the claim 1 to 19, any of the two 1. Method as requested. Rights in any of the rights 1 to 19 It also includes the production of vaccines to prevent flu. Where the vaccine consists of the harvested virus 2 2. Any method of claim 1 to 19 also includes the production of the vaccine. To prevent a pandemic of human flu Where the vaccine contains collected virus 2 3. Any method by which Claim No. 1 to 19 is claimed. Also includes vaccine production For the prevention of human flu, where the vaccine contains collected virus 2 4. Any one of the methods for which claims 1 to 19 are made also include the production of vaccines for protection. Flu in equine, pig, member of canine, member of feline, minke (mustelids), and bird species. Where the vaccine contains collected viruses. 2 5. The use of eggs from hens immunized against flu. For the production of flu virus Where the use included the implantation of the flu virus into the egg's Callantoic Cavite, incubating the eggs under temperature conditions. And the right humidity In order to increase the viral load, and to collect the virus from the Cullantoic fluid 2 6. Use as requested in claim 25 also includes the production of a flu vaccine, which includes the virus collected. 2. 7. Use as requested. Clause 25 or 26, where the virus, contains antibodies to hemagglutinin of influenza virus directly. 2. 8. Use as requested. 27 where direct influenza virus haemaclutinin antibodies contain H5, H6, H7 or H9 subtypes. ------------ Revised 07/01/16 1. Method for Produce influenza virus where: a. The flu virus is given to the hen b. Eggs from the immunized hen are collected c. Embryo-stimulated d. The embryo eggs are infected. The flu virus was injected into the allantoic cavity e. Infected egg embryo. Was cured under temperature conditions And suitable humidity And f. To collect allantoic fluid containing the virus 2. Method for claiming claim 1, where the vaccine protects the hen from avian influenza. Holds the right to claim 1 or 2, where the flu vaccine Included Hemagglutinin of influenza virus in protein form And / or the genes that are coded into this protein in Composition of Vaccine 4. Method for Claim No. 3 where the component of the flu vaccine contains the virus. The flu has all the flu viruses that have been inactivated. 5. Method of claiming to claim the rights 1 to 4 any one. Where the composition of the vaccine The flu has a product from the flu virus whole. 6. Method of claiming to claim No. 1 or No. 5, where the composition of the vaccine The adjuvant is also present. 7. Method for claiming rights 1 to 3 where the flu vaccine component contains the virus. The flu already has a weakened flu virus. 8. Methods of claiming rights 1 to 3 where the flu vaccine is composed. Including a vector containing a gene that encodes as a hema glutinin of influenza virus 9. The method of claiming rights in the 8th claim, where this vector is a virus mask 1 0 The method of claiming the right to claim 8 or 9, while VECTOR also includes the genes that Code of flu virus neiramidase 1 1. Method by which one of the claims in clauses 8 to 10 is applied where the composition of the vaccine The adjuvant is as well. 1. 2. Method by requesting the right to claim any of the rights in items 3 to 11, where the haemac glutinin virus Flu is in the form of proteins And / or the genes that are coded into this protein Available as a component of the hens vaccine and hemagglutinin of the flu virus used to make Allan. Toiccaventy in eggs that have become embryo from Immunosuppressed hens have different subtypes. 1 3. Any method of claiming 3 to 11. Where the hematocrit of the virus Flu is in the form of proteins And / or the genes that are coded into this protein Contained in the composition of the vaccine that provides hens and hemagglutinin of the flu virus used to make Allan. Toiccaventy in eggs that have become embryo from Hens that have been stimulated, protected and infected have the same subtypes. 1 4. Any of the methods required for claim 3 to 11. Where the hematocrit of the virus Flu is in the form of proteins And / or the genes that process this protein Available as a component of the hen and hemagglutinin vaccine for the flu virus used to make Allan. Toiccaventy in eggs that have become embryo from A hen that has been immune-boosted is infected. Is the same 1 5. Method of the right to claim item 4,6 or 7 where the flu virus is The composition of the vaccine given to the hens is similar to the influenza virus used to infect allantoiccaavism in embryo eggs from immunized hens. Any of the 12 to 15 claims Where the hemagglutinin of The flu virus is in the form of proteins. And / or the genes that process this protein Existing as an element of The vaccine for the hen And hemaclutinin of the flu virus used to make Allan Toiccavity in embryo eggs Then from the hens that were immune and then infected Subtypes of H5, H6, H7 or H9 1. 7. Method in accordance with Claim 16, where the flu's hemagglutinin is in the form of proteins and / or genes. This protein code is contained in the composition of the vaccine used to stimulate it. Hens immunity against flu And hemaclutinin of the flu virus used to make Allan Toik Cavity in Eggs The embryo from the infected hen has an H5 or H7 subtype 1. 8. Methods as requested in Claims 1 to 17, which includes the procedure. One step further Of the virus purification process 1 9. Any method of claiming rights 1 to 18. Assembly steps One step further Of the virus inactivation process 2 0. Flu vaccine obtained by using the method as requested in Clause 1 to 19, any of 2 1. Using the method as requested in claim 1 To 19 any of the above For vaccine production To prevent flu. 2. 2. Using the method as requested in the claim 1 to 19, any one. For vaccine production To prevent a pandemic of influenza in humans. 2. 3. Using the method requested by claiming rights 1 to 19 any one. For vaccine production To prevent the spread of influenza in humans. 2 4. Use of the method requested by Claim No. 1 to 19, one for vaccine production. To prevent flu in animals like horses, pigs, same family as dogs, cats, mink And animal species related to birds 2 5. The use of eggs from hens that are immune to flu. For the production of influenza virus 2 6. The use of eggs from hens that were immune to flu. For flu vaccine production 2 7. Use of eggs as claimed in claim 25 or 26, where the eggs contain antibodies to Flu virus haemaclutinin 2 8. Use of the method as requested in claim 27 where antibodies against viral hemaglutinin Flu subtype H5, H6, H7 or H9 --------------------------------------- --------------------------- 1. Method for producing influenza virus where: a. Flu virus was given to hens b. Egg collection Immune-stimulated hens c. Stimulate embryo process d. Embryo-infected eggs The flu virus was injected into the allantoic cavity e. Infected egg embryo. Was cured under temperature conditions And suitable humidity And f. To collect allantoic fluid containing the virus 2. Method for claiming claim 1, where the vaccine protects the hen from avian influenza. Holds the right to claim 1 or 2, where the flu vaccine Included In the composition of the vaccine Hemagglutinin of influenza virus in protein form And / or the transcribed genes Code to this protein 4. Method of claim for claim 3 where the flu vaccine component contains the virus. The flu that has been inactivated. 5. How to claim the rights in the 3rd claim, where the composition of the flu vaccine contains products The whole body of the flu virus 6. Method for claiming the right to claim No. 4 or No. 5, provided that the composition of the vaccine contains adjuvants. 7. Method for claiming the right to claim No. 3 by Where the components of the flu vaccine contain the virus The flu is already softened. 8. Method for claiming rights 3 where the flu vaccine consists of a vector that Contains nucleic acid fragments coded as haemagglutinin of influenza virus 9. Method as claimed in Claim 8, where this vector is a virus mask 1 0. The method of claiming the right to claim 8 or 9, where the vector is also composed of acid chunks. The nucleic code that is coded as nucleicides of the influenza virus 1 1. Method by which one of the right to claim any of the rights in clauses 8 to 10, where the composition of The vaccine contains adjuvants. 1. 2. Method by which one of the claims in clauses 3 to 11 is claimed, where the haemagglutinin of The flu virus is in the form of proteins. And / or the genes that process this protein Existing as an element Of vaccines used to stimulate the immunity of chickens against the flu And the hemagglutinin of the flu virus used to infect allantoicquiti in the embryo eggs. There are different subtypes 1 3. Method by which one of the claims in clause 3 to 11 is requested, whereby the hemagglutinin of The flu virus is in the form of proteins. And / or the genes that process this protein Existing as an element Of vaccines used to stimulate the immunity of chickens against the flu And the hemagglutinin of the flu virus used to infect allantoicquiti in the embryo eggs. The subtype is the same. 1 4. The method in which one of the right to claim any of the rights in items 3 to 11 is that the hemagglutinin of The flu virus is in the form of proteins. And / or the genes that process this protein Existing as an element Of vaccines used to stimulate the immunity of chickens against the flu And the hemagglutinin of influenza virus used to cause allantoicquiti in embryo eggs from immunosuppressed hens are infected is the same. 1 5. As requested. Holds the right to any of the rights in items 4,6 or 7, where the flu virus In Section 5 of the vaccine used to stimulate the immunity of the hen against the flu. And viral hematocritin The flu used to infect allantoicquiti in embryo eggs from immunized hens 1 6. Means as requested for one of the claims in item 11. To 14, where the hematocrit of The flu virus is in the form of proteins. And / or the genes that process this protein Existing as an element Of vaccines used to stimulate the immunity of chickens against the flu And hemagglutinin of influenza virus used to cause allantoicquite in embryo eggs from infected immunosuppressed hens have subtypes of H5, H6, H7. Or H9 1. 7. Method as claimed in claim 15 where influenza virus hemagglutinin is in the form of a protein and / or genes that are processed into this protein. Available as a component of the vaccine used to stimulate Hens immunity to flu And hemaclutinin of the flu virus used to make Allan Toffee in an egg Which is an embryo from a hen that has been immune and then infected Subtype of H5 or H7 1. 8. Method of claiming in any one of the right claims in items 1 to 17, including the procedure One step further Of the virus purification process 1 9. Methods in which one of the claims in items 1 to 18 consists of a procedure. One step further Of the virus inactivation process 2 0. Flu vaccine obtained using the method requested in any of the claims 1 to 19 2 1. Use of the method as requested by any of the claims 1 to 19 for vaccine production. To prevent flu 2 2. Use of the method as requested by any of the claims 1 to 19 for vaccine production. To prevent a pandemic of influenza in humans 2 3. Use of the method as requested by any of the claims 1 to 19 for vaccine production. To prevent the spread of flu in humans 2 4. Use of the method requested by any of the claims 1 to 19 for the production of vaccines. To prevent the flu in animals like horses. The same animal as the pig The same family as the dog Animals of the same family as arachnids Mink And species related to birds 2 5. The use of eggs from hens that are immune to the flu. For the production of influenza virus 2 6. The use of eggs from hens that are immune to the flu. For the production of flu vaccine 2 7. Use of the egg, as claimed by claim 25 or 26, where the egg contains antibodies to hematocritin. Of the flu virus 2 8. Use of the eggs as claimed in claim 27, where the eggs contain antibodies to the H5, H6, H7 or H9 subtypes.