TH68412A - A new enzyme for making peptides - Google Patents

Abstract

DC60 (17/11/46) This invention involves a new enzyme. That can easily produce peptides with high yield and low cost. Without the need for a complicated process in detail is Is a type of enzyme New that catalyzes peptide production from Carboxy assembly And amine composition And also involved in microorganisms that produce this enzyme Including a presentation of the method Using microorganisms or enzymes In the production of diapers Tide at low cost The inventors discovered a new strain of bacteria in the genus Empedobacter that can produce a new enzyme efficiently. High efficiency And production methods that use such bacteria The invention involved a new enzyme. That can easily produce peptides with high yield and low cost. Without the need for a complicated process in detail is Is a type of enzyme New that catalyzes peptide production from Carboxy assembly And amine composition And also involved in microorganisms that produce this enzyme Including a presentation of the method Using microorganisms or enzymes In the production of diapers Tide at low cost The inventors discovered a new strain of bacteria in the genus Empedobacter that can produce a new enzyme efficiently. High efficiency And production methods that use such bacteria

Claims (30)

1. DNA that encodes the proteins in (A) and (B) as follows: (A) Proteins with amino acid sequences that match amino acid sequences 23-616 in sequence number 6 from the sequence list table. (B) Proteins with amino acid sequences that match amino acid sequences 23-616 in sequence number 6 from the sequence list table, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while still maintaining the bioactivity in peptide synthesis. 2. DNA that encodes the proteins in (C) and (D) as follows: (C) Proteins with amino acid sequences that match amino acid sequences 21-619 in sequence number 12 from the sequence catalog; (D) Proteins with amino acid sequences that match amino acid sequences 21-616 in sequence number 12 from the sequence catalog, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while still maintaining the bioavailability in peptide synthesis. 3. DNA that encodes the proteins in (E) and (F) as follows: (E) Proteins with amino acid sequences that match amino acid sequences 23-625 in sequence number 18 from the sequence list table. (F) Proteins with amino acid sequences that match amino acid sequences 23-625 in sequence number 18 from the sequence list table, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while still maintaining bioactivity in peptide synthesis. 4. DNA that encodes the proteins in (G) and (H) as follows: (G) Proteins with an amino acid sequence that matches amino acid sequence 23-645 in sequence number 23 from the sequence list table. (H) Proteins with an amino acid sequence that matches amino acid sequence 23-645 in sequence number 23 from the sequence list table, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while still maintaining bioavailability in peptide synthesis. 5. DNA that encodes the proteins in (I) and (J) as follows: (I) Proteins with amino acid sequences that match amino acid sequences 26-620 in sequence number 25 from the sequence list table. (J) Proteins with amino acid sequences that match amino acid sequences 26-620 in sequence number 25 from the sequence list table, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while still maintaining bioactivity in peptide synthesis. 6. DNA that encodes the proteins in (K) and (L) as follows: (K) Proteins with amino acid sequences that match amino acid sequences 18-644 in sequence number 27 from the sequence catalog. (L) Proteins with amino acid sequences that match amino acid sequences 18-644 in sequence number 27 from the sequence catalog, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while still maintaining bioavailability in peptide synthesis. 7. DNA that encodes the proteins in (M) and (N) as follows: (M) Protein whose amino acid sequence matches sequence number 6 from the sequence table; (N) Active protein whose amino acid sequence matches sequence number 6 from the sequence table, which may contain one or more substitutions, omissions, insertions, additions, and/or swaps, while maintaining bioactivity in peptide synthesis. 8. DNA that encodes the proteins in (O) and (P) as follows: (O) Protein whose amino acid sequence matches sequence number 12 from the sequence table. (P) Active protein whose amino acid sequence matches sequence number 12 from the sequence table, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while still maintaining bioactivity in peptide synthesis. 9. DNA that encodes the proteins in (Q) and (R) as follows: (Q) A protein whose amino acid sequence matches sequence number 18 from the sequence table. (R) An active protein whose amino acid sequence matches sequence number 18 from the sequence table, which may contain one or more substitutions, omissions, insertions, additions, and/or swaps, while maintaining its bioactivity in peptide synthesis. 10. DNA that encodes the proteins in (S) and (T) as follows: (S) Protein with an amino acid sequence matching sequence number 23 from the sequence table; (T) Active protein with an amino acid sequence matching sequence number 23 from the sequence table, which may contain one or more substitutions, deficiencies, insertions, additions, and/or swaps, while maintaining bioactivity in peptide synthesis. 11. DNA that encodes the proteins in (U) and (V) as follows: (U) Proteins with an amino acid sequence that matches sequence number 25 from the Sequence List Table; (V) Active proteins with an amino acid sequence that matches sequence number 25 from the Sequence List Table, where there may be one or more substitutions, deficiencies, insertions, additions, and/or swaps, while maintaining bioactivity in peptide synthesis. 12. DNA that encodes the proteins in (W) and (X) as follows: (W) Protein whose amino acid sequence matches sequence number 27 from the Sequence List Table; (X) Active protein whose amino acid sequence matches sequence number 27 from the Sequence List Table, where there may be one or more substitutions, omissions, insertions, additions, and/or swaps, while maintaining bioactivity in peptide synthesis. 13. DNA shown in (a) and (b) as follows: (a) DNA with a base sequence matching the base sequences 127-1908 in sequence number 5 from the sequence list table; (b) DNA that can undergo hybridization with DNA obtained from the complementary bases of the base sequences 127-1908 in sequence number 5 from the sequence list table under specific conditions and can encode proteins that have bioactive peptide-building properties. 14. DNA shown in (c) and (d) as follows: (c) DNA whose base sequence corresponds to base sequences 121-1917 in sequence number 11 from the sequence list table; (d) DNA that can hybridize with DNA obtained from the complementary bases of the base sequences 121-1917 in sequence number 11 from the sequence list table under specific conditions and can encode proteins that have bioactive peptide-building properties. 15. DNA shown in (e) and (f) as follows: (e) DNA with a base sequence matching the base sequences 127-1935 in sequence number 17 from the sequence list table; (f) DNA that can hybridize with DNA obtained from the base pairs of the base sequences 127-1935 in sequence number 17 from the sequence list table under specific conditions and can transcribe proteins that have bioactive peptide-building properties. 16. DNA shown in (g) and (h) as follows: (g) DNA with a base sequence matching sequence 127-1995 in sequence number 22 from the sequence list table; (h) DNA that can undergo hybridization with DNA obtained from the complementary bases of sequence 127-1995 in sequence number 22 from the sequence list table under specific conditions and can encode proteins that have bioactive peptide-building properties. 17. DNA shown in (i) and (j) as follows: (i) DNA with a base sequence matching the base sequences 104-1888 in sequence number 24 from the sequence list table; (j) DNA that can hybridize with DNA obtained from the base pairs of the base sequences 104-1888 in sequence number 24 from the sequence list table under specific conditions and can transcribe proteins that have bioactive peptide-building properties. 18. DNA shown in (k) and (l) as follows: (k) DNA whose base sequence corresponds to the base sequences 112-1992 in sequence number 26 from the sequence list table; (l) DNA that can undergo hybridization with DNA obtained from the base pairs of the base sequences 112-1992 in sequence number 26 from the sequence list table under specific conditions and can encode proteins that have bioactive peptide-building properties. 19. DNA shown in (m) and (n) as follows: (m) DNA whose base sequence corresponds to the base sequences 61-1908 in sequence number 5 from the sequence list table; (n) DNA that can undergo hybridization with DNA obtained from the base pairs of the base sequences 61-1908 in sequence number 5 from the sequence list table under specific conditions and can encode an active protein with peptide-synthesis activity. 20. DNA shown in (o) and (p) as follows: (o) DNA with a base sequence matching the 61-1917 bases in sequence number 11 from the sequence list table; (p) DNA that can hybridize with DNA obtained from the complementary bases of the 61-1917 bases in sequence number 11 from the sequence list table under specific conditions and can transcribe active proteins that have bioactivity in peptide synthesis. 21. DNA shown in (q) and (r) as follows: (q) DNA whose base sequence corresponds to the base sequences 61-1935 in sequence number 17 from the sequence list table; (r) DNA that can hybridize with DNA obtained from the base pairs of the base sequences 61-1935 in sequence number 17 from the sequence list table under specific conditions and can encode an active protein with peptide-synthesis activity. 22. DNA shown in (s) and (t) below: (s) DNA with a base sequence matching the base sequences 127-1995 in sequence number 22 from the sequence list table; (t) DNA that can undergo hybridization with DNA obtained from the base pairs of the base sequences 127-1995 in sequence number 22 from the sequence list table under specific conditions and can encode an active protein with peptide-synthesis activity. 23. DNA shown in (u) and (v) as follows: (u) DNA with a base sequence matching the 29-1888 base pair in sequence number 24 from the sequence list table; (v) DNA that can undergo hybridization with DNA obtained from the base pairs of the 29-1888 base pair in sequence number 24 from the sequence list table under specific conditions and can encode an active protein with peptide-synthesis activity. 24. DNA shown in (w) and (x) as follows: (w) DNA whose base sequence corresponds to the base sequences 61-1992 in sequence number 26 from the sequence list table; (x) DNA that can hybridize with DNA obtained from the base pairs of the base sequences 61-1992 in sequence number 26 from the sequence list table under specific conditions and can transcribe an active protein with peptide-synthesis activity. 25. One of the DNA samples from items 13-24 above is reacted under specific conditions, namely, treated with a salt concentration equivalent to 1 x 0.1% SDS at 60°C. 26. Recombinant DNA that is DNA matching any of the categories in points 1-25 above. 27. Transformed cells containing the import of recombinant DNA as described in point 26 above. 28. A method for producing synthetic peptide enzymes characterized by culturing transformant cells from point 27 above in a culture medium and harvesting the enzyme from the fermentation broth and/or cell mass. 29. A distinctive method for producing dipeptides involves culturing transformed cells from point 28 above in a culture medium and then mixing the fermented substances with carboxymethyl and amine components to create dipeptides. 30. A method for producing dipeptides from carboxymethyl and amine components is characterized by the use of fermentation broth, cell mass isolated from fermentation broth, substances obtained from cell treatment, or enzymes derived from microorganisms of the genus Sphingobacterium, which have the ability to synthesize dipeptides from carboxymethyl and amine components.