TH66992B - Optimization of expression of HPV58 L1 in yeast - Google Patents

Abstract

DC60 (11/11/47) DNA, a synthetic molecule coded as HPV58 L1, a protein that is especially made up of the present invention refer to the polynucleotide code HPV58 L1. Such nucleotide It is a codon that has been optimized for performance. High in yeast cells, such synthetic molecules may It is used to produce particles similar to HPV58 viruses (VLPs) and to form vaccine components and drugs containing HPV58 VLPs. The inventions of vaccines address effective immunosuppressive therapies. immunoprophylaxis) infection with Papilloma virus By using the antibody that has undergone neutralyses And cells that induce immunity and continue to be useful in the treatment of HPV-induced DNA, a synthetic molecule coded as HPV58 L1, a protein that was made, especially the current invention, refers to the polynucleotomy. Tide with the code HPV58 L1, a protein where such polynucleotides It is a codon that has been optimized for performance. High in yeast cells, such synthetic molecules may It is used to produce HPV58 virus-like particles (VLPs) and to make various vaccine and drug components containing HPV58 VLPs. The various vaccines that have been fabricated refer to the therapeutics of the disease. Effective immunoprophylaxis against infection with papilloma virus By using the antibody that has undergone neutralyses And cells that induce immunity and are still useful in the treatment of HPV-induced infections.

Claims (9)

Disclaimer (all) which will not appear on the advertisement page. : ------ 05/04/2018 ------ (OCR) Page 1 of Quantity 1 Page Disclaimer 1. Nucleic acid molecule with code HPV58 L1, protein containing SEQ ID NO: 2 by That molecule The nucleic acid consists of a nucleic acid sequence with the SEQ ID NO: 2 code. Suitable for high expression in yeast cells 2. vector containing nucleic acid molecules According to claim 1 3. Host cells containing vectors According to claim 2, host cell is yeast cell 4. Host cell according to claim 3, where host cell is yeast shell 5. Host shell according to claim 4, where shell yeast is selected from group Containing Saccharomyces cerevisiae, Hansenula polymorpha, Pichia pastoris, Kluyveromyces fragilis, Kluyveromyces lactis and Schizosaccharomyces pombe 6. Host cell according to claim 5, where the host cell is Saccharomyces cerevisiae 7.Nucleic acid molecules According to claim 1, the sequence of the nucleotite acid consists of a sequence. Nucleotite as defined in SEQ ID NO: l. 8. Vantage that contains Nucleic acid molecules According to claim 7 9. The home host cell that resides The host cell is a yeast shell. Containing Vector according to claim 8 ------------ 1. Nucleic acid molecules containing The nucleotide sequence coded as HPV58 L1 protein is defined in SEQ ID NO: 2. Nucleotide sequence. That is a codon that has been modified Suitable for expression at level In yeast cells 2. A vector composed of nucleic acid molecules According to claim 1 3. Host cells containing vectors Proposition 2 4. Host cell according to claim 3 where host cell is yeast cell 5. Host cell according to claim 4 where yeast cell is selected from group containing Saccharomyces cerevisiae, Hansenula polymorpha, Pichia pastoris, Kluyveromyces fragilis, Kluyverom yces lactis and Schizosaccharomyces pombe 6. Host cell according to claim 5, where host cell is Sac charomyces cerevisiae 7. Nucleic acid molecule. According to claim 1, where the nucleic acid etch contains the nucleotide sequence, defined in SEQ ID NO: 1 8. Vector consisting Nucleic acid molecules According to claim No. 7 9. The host cell that contains Vector by claim 8 1 0. Virus-like particles (VLPs) contain recombinant L1 protractor or recombinant L1 + L2, the protein of HPV58, where it recombines. L1 protein or recombinant L1 + L2 protein is formed in st 1 1. VLPs in accordance with claim 10, where L1 recombinant or recombinant protein. L1 + L2 proteins are coded by modulated codons. Optimization of the nucleic acid molecule HPV58 1 2. VLPs according to claim 11, where the modified codon was The appropriateness of the nucleic acid molecule consists of the nucleotide sequence as defined in SEQ ID NO: 1 1 3. Method used to generate VLPs pursuant to claim 11 consisting of (a) Rasformed yeast with DNA molecules containing modified code Formulated with the code HPV58 L1 Protein or HPV58 L1 + L2 Proin (b), cultured of transformed yeast under various conditions that contributed to the expression of cattle-containing DNA molecules. Don optimized to generate recombinant Papilloma virus protein; And (c) recombinant extraction Papilloma virus protein to create VLPs pursuant to claim 11 1 4. Vaccines containing VLPs pursuant to claim 11 1 5. Drug components containing VLPs pursuant to claim 11 1 6. Method Prevention of HPV infection that consists of Ccine in claim 14 for mammals 1 7. Methods for establishing an immune response in animals. That consists of the provision of VLPs according to claim 11 to animal 1 8. Virus-like particles. According to claim 11, where the yeast can choose from The group consisted of Saccharomyces cerevisiae, Han senula polymorpha, Pichia pastoris, Kluyveromyces fragilis, Klu yveromyces lactis, and Schizosaccharomyces pombe 1 9. Virus-like particles. Pursuant to claim 18, where the yeast is Saccharomyces cerevisiae 2.0. The vaccine according to claim 14 consists of additional VLPs from at least one additional HPV type 2. 1. Vaccine according to claim 20, where at least one additional HPV type was selected. From the categories that include: HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV5 2, HPV55, HPV56, HPV59, and HPV68 2. 2. Vaccine according to claim 21, where at least one type of HPV is made up of HPV16 2. 3. Vaccine according to claim 22, supplemented with HPV18 VLP s 2. 4. The vaccine according to claim 23 contains additional HPV6 VLPs and HPV11 VLPs 2. 5. Vaccine according to claim 24, supplemented with HPV31 VL Ps 2. 6. Vaccine according to claim 23, supplemented with HPV31 VL Ps 2. 7. Vaccine according to claim 25, supplemented with HPV45 VL Ps 2. 8. Vaccine according to claim 26, supplemented with HPV45 VL Ps 2. 9. The extractable and purified HPV58 L1 polypeptide is composed of various amino acid sequences as defined in SEQ ID ON: 2.