TH66992B - Optimization of expression of HPV58 L1 in yeast - Google Patents

Abstract

DC60 (11/11/47) DNA, a synthetic molecule coded as HPV58 L1, a protein that is especially made up of the present invention refer to the polynucleotide code HPV58 L1. Such nucleotide It is a codon that has been optimized for performance. High in yeast cells, such synthetic molecules may It is used to produce particles similar to HPV58 viruses (VLPs) and to form vaccine components and drugs containing HPV58 VLPs. The inventions of vaccines address effective immunosuppressive therapies. immunoprophylaxis) infection with Papilloma virus By using the antibody that has undergone neutralyses And cells that induce immunity and continue to be useful in the treatment of HPV-induced DNA, a synthetic molecule coded as HPV58 L1, a protein that was made, especially the current invention, refers to the polynucleotomy. Tide with the code HPV58 L1, a protein where such polynucleotides It is a codon that has been optimized for performance. High in yeast cells, such synthetic molecules may It is used to produce HPV58 virus-like particles (VLPs) and to make various vaccine and drug components containing HPV58 VLPs. The various vaccines that have been fabricated refer to the therapeutics of the disease. Effective immunoprophylaxis against infection with papilloma virus By using the antibody that has undergone neutralyses And cells that induce immunity and are still useful in the treatment of HPV-induced infections.

Claims (29)

------05/04/2561------(OCR) Page 1 of 1 page. Claims. 1. A nucleic acid molecule coded as HPV58 L1 protein containing SEQ ID NO:2, where the nucleic acid sequence with SEQ ID NO:2 has a codon modified to be suitable for high expression levels in yeast cells. 2. A vector containing nucleic acid molecules, as per claim 1. 3. Host cell containing the vector, according to claim 2, whereby the host cell is a yeast cell. 4. Host cell according to claim 3, where the host cell is a yeast cell. 5. The host cell according to claim 4, which states that yeast cells can be selected from groups consisting of Saccharomyces cerevisiae, Hansenula polymorpha, Pichia pastoris, Kluyveromyces fragilis, Kluyveromyces lactis, and Schizosaccharomyces pombe. 6. The host cell according to claim 5, where the host cell is Saccharomyces cerevisiae. 7. A nucleic acid molecule, according to claim 1, in which the nucleotide sequence consists of the nucleotide sequence specified in SEQ ID NO:l. 8. A space containing nucleic acid molecules, as per claim 7. 9. Host cell, where the host cell is a yeast cell, containing a vector according to claim 8 ------------ 1. A nucleic acid molecule containing the nucleotide sequence coded as HPV58 L1 protein as defined in SEQ ID NO:2. The nucleic acid sequence is a codon optimized for high expression levels in yeast cells. 2. A vector containing a nucleic acid molecule according to claim 1. 3. A host cell containing a vector according to claim 2. 4. A host cell according to claim 3, where the host cell is a yeast cell. 5. A host cell according to claim 4, where the yeast cell is selected from a group consisting of... 6. A host cell according to claim 5, where the host cell is Saccharomyces cerevisiae, Hansenula polymorpha, Pichia pastoris, Kluyveromyces fragilis, Kluyveromyces lactis, and Schizosaccharomyces pombe. 7. A nucleic acid molecule according to claim 1, where the nucleic acid otode sequence consists of the nucleotide sequence specified in SEQ ID NO:1. 8. A vector containing the nucleic acid molecule according to claim 7. 9. A host cell containing the vector according to claim 8. 10. Viral-like particles (VLPs) consist of recombinant L1 protein or recombinant L1+L2 proteins of HPV58, where the recombinant L1 protein or recombinant L1+L2 proteins are synthesized in the yeast. 11. VLPs pursuant to claim 10, where recombinant L1 protein or recombinant L1+L2 protein is encoded by a modified codon of the nucleic acid molecule HPV58. 12. VLPs pursuant to claim 11, where the optimized codon of a nucleic acid molecule contains the nucleotide sequence as defined in SEQ ID NO:1. 13. Methods used to construct VLPs according to claim 11 include: (a) transformation of yeast with DNA molecules containing modified codons encoding HPV58 L1 protein or HPV58 L1 + L2 protein; (b) cultivation of the transformed yeast under various conditions conducive to the expression of DNA molecules containing modified codons to construct recombinant papilloma virus proteins; and (c) isolation of recombinant papilloma virus proteins to construct VLPs according to claim 11. 14. Vaccines containing VLPs as per claim 11. 15. Pharmaceutical components containing VLPs as per claim 11. 16. Methods used to prevent HPV infection include administering the vaccine under Patent 14 to mammals. 17. A method for inducing an immune response in animals, consisting of administering VLPs as per Claim 11 to the animals. 18. Virus-like particles, according to claim 11, which yeast is selected from the group consisting of Saccharomyces cerevisiae, Han senula polymorpha, Pichia pastoris, Kluyveromyces fragilis, Kluyveromyces lactis, and Schizosaccharomyces pombe. 19. Virus-like particles, according to claim 18, where yeast includes Saccharomyces cerevisiae. 20. The vaccine under claim 14 is additionally composed of VLPs from at least one additional HPV species. 21. A vaccine under claim 20 in which at least one additional HPV variant has been selected from a pool comprising: HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV55, HPV56, HPV59, and HPV68. 22. Vaccines under claim 21 in which at least one HPV species is included, namely HPV16. 23. The vaccine under claim 22 includes additional HPV18 VLPs. 24. The vaccines under claim 23 include additional HPV6 VLPs and HPV11 VLPs. 25. The vaccine under claim 24 includes additional HPV31 VL Ps. 26. The vaccine under claim 23 includes additional HPV31 VL Ps. 27. The vaccine under claim 25 includes additional HPV45 VL Ps. 28. The vaccine under claim 26 includes additional HPV45 VL Ps. 29. The extracted and purified HPV58 L1 polypeptide contains the amino acid sequences as specified in SEQ ID ON:2.