TH62912B - A process for extraction and recovery of polyhydroxyl alcohol (PHAs) from cellular biomass. - Google Patents

Abstract

DC60 (02/03/60) Process for extraction and polyhydroxyalkanoaates (PHAs) were recovered from biomass. of cells that comprise the steps of Solvent extraction of polyhydroxyalkanate At least one type of non-haedolginated. which does not destroy the environment; heating the biomass of cells, to form a suspension consisting of a solvent PHA containing dissolved PHA and the remaining undissolved; recovery of PHA-filled solvents; injecting a solvent filled with PHA in a vapor stream to induce a rapid precipitation of PHA in water, while evaporating the solvent; complete elimination of residual solvent; Separation of pure PHA particles from suspensions and bring those substances to dry. Edit 02/03/2017 Process for extraction and polyhydroxyalkanoaates (PHAs) were recovered from biomass. of cells composed of a step of: solvent extraction of polyhydroxyalkanate. One or more non-halogenated which does not harm the environment;heating the biomass of the cell, to form a PHA-containing suspension containing dissolved PHA and the remaining undissolved; recovery of PHA-filled solvents; injecting a solvent filled with PHA in the vapor stream to induce a rapid precipitation of PHA in water, while evaporating the solvent; complete elimination of residual solvent; Separation of pure PHA particles from suspensions and bring those substances to dry ------------------- Process for extraction and polyhydroxyalkanoaates (PHAs) were recovered from biomass. of cells that comprise the steps of Solvent extraction of polyhydroxyalkanate At least one type of non-haedolginated. which does not destroy the environment; heating the biomass of cells, to form a PHA-containing suspension containing dissolved PHA and the remaining undissolved; recovery of solvent enriched with PHA; Solvent injection that is enriched with PHA in the vapor stream to induce a rapid precipitation of PHA in water, while performing the solvent evaporation process, the complete elimination of the residual solvent; Separation of pure PHA particles from suspensions and dried them:

Claims (2)

Disclaimer (all) which will not appear on the advertisement page: EDIT 02/03/2017 1. Process for extraction. And the recovery of polyhydroxyl alcohol (PHAs) from the biomass of bacterial cells, by fermentation, which is characterized by This process includes steps of: i) the tandem implementation of cell biomass of PHA solvent injection, strong agitation. And rapid heating in the interior of the reactor to Stimulate the tear of the biomass cell wall and the dissolution of the PHA in the posterior, and to make Formed a PHA-containing suspension containing a PHA filled with a dissolved PHA. Concentrated at least 2% by weight of the suspension, and the insoluble residue of cell biomass, where the PHA solvent used was selected from the methylformate-containing group of solvents. , Ethylformate, propylformate, butylformate, isomylformate, pentylphosphate Formiates (amyl) and caproylformates (or hexyl) and their mixtures, ethyl propionate, isopo Propyl propionate, isoammyl propionate, caproyl propionate (or hexyl) and their mixtures, methylbionate V Tirate, Propyl Butyrate, Isopropyl Butyrate, Isoammyl Butyrate, Pentyl Butyrate (Amyl), Caproi Butyrate (or hexyl) and its mixtures, methyl isobutyrate, propyl isobutyrate, isopropyl isobutyl. Vutylorate, Isomyl Isobutyrate, Pentyl Isobutyrate (Amyl), Caproyl Isobutirate (or Hexyl) And mixtures of these solvents, propyl valerate, isopropyl valerate, butyl valerate, isobutyl valerate, isoamylva. Lerate (3-methyl-1-butyl), pentyl valerate (amyl) and caproyl valerate (or hexyl) and request. The mixture of these solvents and methyl isoamylate (isovalerate) (3-methyl-1-methyl butanol), ethyl isoacetate. Milate (Isovalerate) (3-methyl-1-ethyl butanolate), propyl isoamylate (isovalerate) (3-methyl -1-propyl butanolate), isopropyl isoamylate (isovalerate) (3-methyl-1-isopropyl butanolate T), butyl isoamylate (isovalerate) (3-methyl-1-butyl-butanolate), isobutyl isoamic Leth (Isovalate) (3-methyl-1-isobutyl-butanolate), Isoamyl isoamylate (Isovalurate) (3 -Methyl-1-ishoamyl butanolate), pentyl isoamylate (isovalerate) (amyl) (3-methyl-1- Pentyl or amylbutanolate) and daproyl isoamylate (isovalerate) (hexyl), and a mixture of these solvents; ii) the introduction of the suspended solids in the reactor to the separation process for water Solvent filled with PHA recovered from the insoluble residue of biomass of Remaining cells; iii) A solution of PHA-filled PHA solvent to stirring and washing operations with water and / or steam at a temperature sufficient and appropriate to stimulate removal, by evaporation, all PHA solvents. Significantly and PHA precipitation, to form a PHA-containing suspension precipitated in water. And the remaining solvent; iv) The introduction of the precipitated PHA suspension to the remaining solvent evaporation. Until they are depleted by injecting steam in the said PHA suspension, to obtain purified PHA particles in the Aqueous suspension; v) cooling the suspended solids in the ACE medium, where the PHA solvent is removed from the suspension, to approximately 45 ํ or less, and the separation of PHA particles from the suspension; And vi) the introduction of the separated PHA particles into the drying process 2. The process described in claim 1, which is characterized by the PHA solvent used is selected from a group of solvents consisting of: Isoammyl propionate, propyl butyrate, isoamyl valerate and isoammyl isoamylate or mixtures of these substances. Described in claim 1, whose characteristic is that the PHA solvent is the isomyl propionate 4. The process described in claim 1, which has a unique characteristic is the PHA solvent. 5. The process described in Clause 1, which is unique, is the PHA used to be selected. From the groups defined by poly-3-hydroxybutyrate (PHB), poly (hydroxybutyrate-co-hydroxylvalerate), PHBV and its mixtures Polymer 6. The process described in claim 5, which is unique is that PHA is produced by bacterial fermentation, using microorganisms which can be biosynthetic using PHA, extracted sugar. From the beginning Sugar cane is a staple And is characterized by the main energy source used To generate energy Heating and electric power The process is preferred by bagasse 7. The process described in claim 1, which is unique is the injection process. Solvents in concentrated cell biomass include Injection operation of liquid PHA solvent and PHA solvent in vapor form, to stimulate cell biomass heating. At a temperature between approximately 90 ํ and the boiling temperature of the solvent at atmospheric pressure is significant. 8. The process described in claim 1, is characterized by the elimination. The solvent from the PHA solution was processed in the evaporation chamber under grinding the PHA solution in a steam stream, followed by PHA precipitation in the aqueous mixture. 9. The process described in Clause 8, which is characterized by steam used for Solvent removal from PHA solution by evaporation, by direct injection of the solution in a steam stream, is the superheated steam relative to the operating pressure in the evaporation chamber 1 0. The process described in the hold. Article 8, which is characterized by elimination The solvent from the PHA solution was processed by injecting the solution directly into the steam stream and into the evaporation channel. Maintained at pressure below atmospheric pressure 1 1. The process described in claim 8, which is characterized by the elimination of The solvent from the PHA solution was performed by injecting the solution directly in the steam stream and in the evaporation chamber maintained at a significant atmospheric pressure 1 2. The process described in Clause 8. Which is characterized by the removal of The solvent from the PHA solution is composed by direct injection of the solution in water and steam and heating under stirring in a pressure stabilizer. 1 3. The process described in Clause 8, which is characterized by the elimination of The solvent from the PHA solution was performed by injecting the solution directly in water and steam, and heating it under stirring in a fixed reagent. At pressure at atmospheric pressure Significantly 1 4. The process described in claim 1, which is unique is the biomass used in The extraction process is in the form of aqueous suspension 1. 5. The process described in claim 14, which is unique is that the PHA solvent induces with water, in the vapor cycle, is a mixed form. Binary 1 6. The process described in Clause 15, which is unique is the injection procedure. Solvents in cell biomass suspensions include Injecting liquid PHA solvent and PHA solvent in vapor form, to induce heating of biomass. Of cells to a temperature between approximately 90 ํ and the boiling temperature of the solvent at the pressure Significant atmosphere And to cause: the liquid phase containing PHA solvent filled with PHA and residual water of cellular biomass; The solid phase is defined by the rest that does not. Dissolution of the residual cell biomass; And the steam cycle with steam And solvent vapor PHA 1 7. The process described in Clause 16, which is unique is this process. It includes a significant additional step of the extraction of the vapor phase. There is a sufficiently large amount of water placed inside the biomass reactor 1 8. The process described in claim 1, which is characterized by cellular biomass. Is derived from natural or genetically modified microorganisms ------------------------ 1. Process for extraction And the polyhydroxyl alcohol (PHAs) returned from biomass. Of bacterial cells, by obtaining such biomass by fermentation, which is characterized by this method Combined with the steps of i) tandem operation of the cell biomass of PHA solvent injection, strong agitation and rapid heating in the interior of the reactor. To stimulate tearing Of the biomass cell wall and the dissolution of PHA in the latter, and to form suspensions that It consists of a PHA solvent added with a PHA dissolved in a concentration of at least 2% by weight of the suspension, and the insoluble residue of the cell biomass. Ii) the introduction of the suspended solids in the heater. Reaction to the separation step for lead Dissolved PHA disruptor from the insoluble residue of residual cell biomass; iii) The introduction of a solution of PHA-enriched solvent by PHA to operation. Stirring and washing with water And / or steam at a temperature which is sufficient And suitable to stimulate removal, by evaporation, all classified PHA solvents And the PHA precipitation, to cause A suspended solids containing PHA precipitated in water. And the remaining solvent; iv) The introduction of the precipitated PHA suspension to the remaining solvent evaporation. Until they are depleted by steam injection in the said PHA suspension, in order to obtain purified PHA particles in the Aqueous suspension. V) Cool the suspension in the Aqueous medium. , Where the PHA solvent was removed, to approximately 45 ํ or less, and separation of PHA particles from the suspended solids; and vi) the separation of the PHA particles was removed to the drying process. Described in claim 1, which is unique is that the PHA solvent used is selected from a group of solvents consisting of: methylformate, ethylformate, Propylformate, butylformate, isoimalformate, pentylformate (amyl) and Propylformate (or hexyl) and a mixture of these solvents, preferably, propylformate, butylformate Eight, isoamicformate, pentylformate and, which should be more preferable, isoimalformate 3.Process according to Described in claim 1, which is unique is that the PHA solvent used is selected from a group of solvents consisting of: ethyl propionate, isopropyl propionate, Isoamyl propionate, caproylo Propionate (or hexyl) and a mixture of these solvents and, which is preferable, isopropyl propionate and isoammyl propionate, And, which is preferable to use, isoamyl propionate 4. The process described in claim 1, which is unique is that the PHA solvent used is selected from the group of solvents. Solubility containing: methyl butyrate, propyl butyrate, isopropyl butyrate, isoamic butyrate, pentyl butyrate (A Mil), caproyl butyrate (or hexyl) and mixtures of these solvents and, more preferable, propyl butyrate, isopropyl bit Vctirates, Isoammyl Butyrates, Pentyl Butyrates (Amyl) and, more preferable, Isoamyl Butyrate (Butyrate Of 3-methyl-butyl) 5. The process described in Clause 1, which is characterized by the PHA solvent used, is selected from the group of solvents containing: methyl. Isobutyrate, propyl isobutyrate, isopropyl isobutyrate, iso-isobutyrate, pentyl isobutyrate Tirate (amyl), caproyl isobutyrate (or hexyl) and mixtures of these solvents and, preferably propyl isobutene. Rating, Isopropyl isobutyrate, isoamyl Isobutyrate, pentyl isobutyrate (amyl), and which should be used more than isobyl isobutyrate (isobutyrate of 3-methyl-butyl) 6. The process described in claim 1, which is unique is that the PHA solvent used is selected from a group of solvents containing: propyl. Valerate, isopropyl valerate, butyl valerate, isobutyl valerate, isoamyl valerate (3-methyl-1-butyl Tyl), pentyl valerate (amyl) and caproyl valerate (or hexyl) and a mixture of these solvents, and which is preferable to use, is propyl. Valerates, Isopropyl Valerates, Butyl Valerates, Isobutyl Valerates, Isobutyl Valerates, Pentyl Valerates (Ami L) And preferably, isoamyl valerate 7. The process described in Clause 1, which is unique is that the PHA solvent used is selected from a group of solvents. Containing: methyl isoamylate (isovalate) (3-methyl-1-methyl butanolate), ethylhisoamylate (isova Lerate) (3-methyl-1-ethyl butanolate), propyl isoamylate (isovalerate) (3-methyl-1-propyl Butanol), isopropyl isoamylate (isovalerate) (3-methyl-1-isopropyl butanolate), buty L isoamylate (isovalerate) (3-methyl-1-butyl-butanolate), isobe Butyl isoamylate (isovalerate) (3-methyl-1-isobutyl-butanolate), isoamyl isoamylate ( Isovalerate) (3-methyl-1-isoammyl butanolate), pentyl isoamylate (isovalerate) (amyl) ( 3-methyl-1-pentyl or amylbutanolate), and caproyl isoamylate (isovalerate) (hexyl), and Mixtures of these solvents And which should be more preferable are propyl isoamylate (isovalate), isopropyl isoamylate (isovalerate), butyl isoamylate ( Isovalate), Isobutyl isoamylate (Isovalerate), Isoamyl isoamylate (Isovalurate), Pentyl isoamylate. Amylate (isovalate) (amil), and preferably used, isoalim isoamylate (isovalate) 8. The process described in the claim Clause 1, which is unique is that the PHA solvent used allows at least approximately 5% by weight to show the solubility of the PHA therein. 9. The process described in Clause 1, which is characterized as The PHA solvent used was selected from a group defined by poly-3-hydroxybutyrate (PHB), poly (hydroxybutyrate-co-hydroxypropylate). ), PHBV and their mixtures of polymer And these copolymers 1 0. The process described in Clause 9, which is characterized by PHA, is produced by Bacteria fermentation, using microorganisms which can be biosynthetic using PHA, as the main raw material, sugar extracted from sugar cane, and the main energy source To produce thermal energy and electrical energy Desired by the process is bagasse 1 1. The process described in Clause 1, which is unique is the injection process. Solvents in concentrated cell biomass include Injection operation of liquid PHA solvent and PHA solvent in vapor form, to stimulate cell biomass heating. At a temperature between approximately 90 ํ and the boiling temperature of the solvent at a pressure rated as Atmospheric pressure 1 2. The process described in claim 1, which is characterized by limiting the solvent from the PHA solution is performed in the evaporation chamber under the pulverization of the PHA solution in the vapor stream. Direct water, followed by PHA precipitation in the aqueous mixture And the condensed solvent 1 3. The process described in Clause 12, which is characterized by the steam used for The solvent is removed from the PHA solution by evaporation, by direct injection of the solution in the steam, is the superheated steam relative to the operating pressure in the evaporation chamber 1 4. The process described in clause 1. Clause 12, which is characterized by the removal of solvent from the PHA solution is performed by injecting the solution in a steam stream. And in the evaporation compartment It is directly maintained at pressure below atmospheric pressure 1 5. The process described in Clause 12, which is characterized by the removal of solvent from PHA solution, is performed by injecting the solution in a steam stream. And in the evaporation compartment Atmospheric pressure 1 6. The process described in Clause 12, which is characterized by the removal of solvents from PHA solutions, consists of injecting an aqueous solution. And direct steam and heating under stirring in a pressure-maintained reagent. 1 7. The process described in claim 12, which is characterized by the removal of solvent from a PHA solution, was performed by injecting an aqueous solution. And direct steam, and heating under stirring in the retained reagent. At pressure classified as atmospheric pressure 1 8. The process described in claim 1, which is unique is the biomass used in the process. The extraction is in the form of an ACQUIUS suspension 1. 9. The process described in Clause 18, which is unique is that the PHA solvent, with water, in the vapor cycle, the bi-mix. Nary 2 0. The process described in Clause 19, which is unique is the injection procedure. Solvents in cell biomass suspensions include Operation of injecting liquid PHA solvent and PHA solvent in vapor form, to induce heating of Cell biomass to a temperature between approximately 90 ํ and the boiling temperature of the solvent at Pressure is classified as atmospheric pressure. And finally produced: liquid cycle containing PHA solvent enriched with PHA and residual water of cellular biomass; Solid phase That is defined by the insoluble residue of the residual cell biomass; And the vapor cycle with steam And solvent vapor PHA 2 1. The process described in Claim 20, which is unique to this process. Includes an additional step of the extraction of the vapor phase that is considered There is more than enough amount of water added. Inside the reactor through biomass 2 2. The process described in claim 1, which is characterized by cellular biomass. Is derived from natural or genetically modified microorganisms