TH5997A - Human reproduction of the protein A-I by expression of the genetic code. - Google Patents

Abstract

The invention resulted in a newly assembled DNA sequence composed of a sequence that defines human AI's propolipoprotein, in which part of the natural-coded sequence was replaced with DNA fragments that define the amino acid code. The same but assembled With different nucleotide sequences, for example, to reduce or prevent Prevents the occurrence of hairpins. Vectors for cloning and vectors showing routing codes These distant DNA are constituents of cells or microorganisms that are genetically modified by vectors. Show these genetic codes And the processes that use them This is for the production of propolis A-I.

Claims (5)

1. The newly incorporated DNA sequence that defines the human AI propolipoprotein code and is able to reduce the occurrence of hairpins consisting of (a) synthetic DNA fragments with nucleotides. Tide (formula) that defines the amino acid code at -6 to +14 of human AI propolypoproteins and (b) the distal end stream of synthetic DNA fragments is the natural DNA sequence that defines the code of Amino acids at +15 to +243 of human AI propolipotin. 2. Recombinant DNA sequences that determine the code of human AI propolipote, where the sequence component defines the natural code. will Are represented by synthetic DNA fragments that define the amine code The same noh but made up of a different nucleotide sequence. In order to reduce or prevent the occurrence of harpins, the incorporated DNA sequence contains a nucleotide sequence (chemical formula). 3. A genetic code carrier consisting of a DNA sequence that is recombined according to The location of claim 1 or 2 and the controlled DNA sequence, which controls the expression of the genetic code of the newly incorporated DNA sequence 4. The carrier of the genetic code as requested in claim 3, which There is a control DNA sequence consisting of the phage lampka, PL, pomotor region. 5. The genetic code carrier as requested in claim 3, with the newly formed sequence, is connected to it. At the end of the beta-galactosicase DNA sequence, and where the control DNA sequence is composed of the lacromooster region, 6. The carrier shows the genetic code as requested in claim 3, which is the sequence. Regulatory DNA consists of the yeast promoter ARG3 region and the transcription cessation zone. 7. The genetic code vectors as requested in claim 3, where the newly formed DNA sequences lack the ATG cocon, are bridged. Connected through the end of E.Coll's Qmpa protein Qmpa DNA sequence stream, and the controller DNA sequence consists of the lpp, lac-pomotor-operator, and lac I sequence of the lac rea. TRESSER 8. The carrier expresses the genetic code as requested in claim 3, which contains the regulated DNA sequence comprising the Pomo region. Vector of the polyhecrin gene of baculovirus 9. cultured cells or microorganisms that have been genetically altered. The vectors show the genetic code in accordance with claim 3 1 0. Cultured cells or microorganisms that have been genetically altered Then by the carrier showing the genetic code in accordance with Claim No. 4 1 1. Cultured cells or microorganisms that have been genetically altered And then by the carrier showing the genetic code according to claim number 5 1 2. Cultured cells or microorganisms that have been genetically altered Then by the carrier showing the genetic code in accordance with Claim No. 6 1 3. Cultured cells or microorganisms that have been genetically altered And then by the carrier showing the genetic code according to claim number 7 1 4. Cultured cells or microorganisms that have been genetically altered And then by the carrier showing the genetic code according to the claim of claim 8 1 5. Process for the production of human A-I propolis, which consists of cultured cells or microorganisms. Mutated Karma, then according to the claim in claim 9 under the condition Appropriate cultures and separation of the propolypoproteins A-I of the produced ones (15 claims, 4 pages, 0 photos).