TH59159A - Bacteria that produce L-amino acids and methods for L-amino acid production. - Google Patents

Abstract

DC60 (21/03/43) L-amino acids are produced by culturing Methylophilus bacteria, which can grow using methanol as the primary carbon source and are capable of producing L-amino acids. For example, Methylophilus bacteria in which dihydrodipicolinate synthase and aspartodinase functions are dominant through mutation via the introduction of DNA transcribed for dihydrodipicolinate synthase, which is not susceptible to reinhibition by L-lysine, or Methylophilus bacteria that are fed a specific casamino acid medium in a methanol-containing medium to produce and accumulate L-amino acids during cultivation. L-amino acids are produced by culturing Methylophilus bacteria, which can grow using methanol as the primary carbon source and are capable of producing L-amino acids. For example, Methylophilus bacteria... Where dihydrodipicolinate synthase and aspartodine functions are promoted by transfection through the introduction of DNA encoded for dihydrodipicolinate synthase, which is not susceptible to reinhibition by L-lysine, or Methylophilus bacteria that are fed a specific casamino acid medium in a methanol-based medium to produce and accumulate L-amino acids in culture, and L-amino acid reabsorption from culture.

Claims (25)

1. Methylophilus bacteria are capable of producing L-amino acids. 2. Methylophilus bacteria as per claim 1, where the L-amino acid is L-lysine, L-valine, L-isoleucine, or L-threonine. 3. Methylophilus bacteria according to claim 1, which exhibit resistance to L-amino acid-like or L-amino acid-specific agents. 4. Methylophilus bacteria, according to claim 1, where the enzymatic function in which L-amino acid profiles are prominent is synthesized. 5. Methylophilus bacteria according to claim 1, where dihydrodipicolinate synthase and aspartodirase functions are enhanced and the bacteria are capable of producing L-lysine. 6. Methylophilus bacteria according to claim 1, where dihydrodipicolinate sintase is dominant and the bacteria are capable of producing L-lysine. 7. Methylophilus bacteria according to claim 1, where aspartodine function is dominant and the bacteria are capable of producing L-lysine. 8. Methyphilus bacteria, according to one of the claims 5 through 7, where the activity of one, two or three selective enzymes of aspartic acid, semialdehyde dehydrogenase, dihydrodipicolinate reductase and dianenopimylate decarboxylase is dominant. 9. Methylophilus bacteria, according to claim 5, where dihydropicolinate synthase and aspartodine functions are asserted by transfection via import into cells of DNA transcribed for dihydropicolinate synthase unaffected by reinhibition by L-lysine and DNA transcribed for aspartodine unaffected by reinhibition by L-lysine. 10. Methylophilus bacteria, according to claim 1, by the dominance of aspartote dienase, hymoserine dehydrogenase, hymoserine dienase, and threonine sinae, and the bacteria are able to produce L-threonine. 11. Any one of the bacteria in claims 1 through 10, where Methylophilus is Methyphilus methylotrphus. 12. A method for the production of L-amino acids, which involves the cultivation of Methylphilus bacteria as defined in any one of Reservices 1 through 11, in a medium for the production and accumulation of L-amino acids, the cultivation and collection of L-amino acids from the culture. 13. Method under Claim 12, where the intermediate has methanol as the primary carbon source. 14. A method for the production of bacterial cells of Methylophilus bacteria with an L-amino acid enrichment fraction consisting of the culture of Methylophilus bacteria as defined in any one of Patents 1 through 11 in a medium for the production and accumulation of L-amino acids in bacterial cells of Methylophilus bacteria. 15. Method for the production of bacterial cells of Methylophilus bacteria according to claim 14, where the L-amino acid is L-lysine, L-valine, L-isoleucine, or L-threonine. 16. DNA that is transcribed for the protein specified in (A) or (B) below: (A) a protein in which the amino acid sequence is: 6, or (B) a protein in which the amino acid sequence is: 6 that includes the substitution, deletion, addition, addition or inversion of one or more amino acids and has the function of aspartodine. 17. DNA pursuant to claim 16, which is DNA defined in (a) or (b) the following: (a) DNA in which a nucleotide sequence is made up of nucleotides 510 to 1736 of sequence: 5, or (b) DNA in which a nucleotide sequence is made up of nucleotides 510 to 1736 of sequence: 5, or a portion of it under strict conditions and is transcribed for a protein that functions aspartodines. 18. DNA that is transcribed for the protein specified in (c) or (d) below: (C) a protein in which the amino acid sequence is: 8, or (D) a protein in which the amino acid sequence is: 8 that includes a substitution, deletion, addition, addition or reversal of one or more amino acids and has aspartic acid semialdehyde dehydrogenase function. 19. DNA pursuant to claim 19, which is the DNA defined in (c) or (d) as follows: (c) DNA which contains a nucleotide sequence consisting of nucleotides 98 to 1207 of sequence: 7, or (d) DNA which can be recombined with a test subject containing a nucleotide sequence of nucleotides 98 to 1207 of sequence: 7 or a part of it under strict conditions and encodes a protein that functions aspartic acid semialdehyde dehydrogenase. 20. DNA that is transcribed for the protein specified in (E) or (F) below: (E) a protein in which the amino acid sequence is: 10, or (F) a protein in which the amino acid sequence is: 10 that includes the substitution, deletion, addition, addition or reversal of one or more amino acids and has the dihydrodipicolinate synthase function. 21. DNA pursuant to claim 20, which is DNA defined in (e) or (f) as follows: (e) DNA in which the nucleotide sequence contains the nucleotide sequences 1268 to 2155 of sequence 9, or (f) DNA in which the nucleotide sequence contains the nucleotide sequences 1268 to 2155 of sequence 9 or a part of it under strict conditions and is transcribed for a protein that functions as dihydrodipicolinate synthase. 22. DNA that is transcribed for a protein designated in (G) or (H) as follows: (G) a protein in which the amino acid sequence of the order is: 12, or (H) a protein in which the amino acid sequence of the order of the order: 12 includes a substitution, deletion, addition, addition or reversal of one or more amino acids and has the function of dihydrodipicolinate reductase. 23. DNA pursuant to claim 22, which is defined as (g) or (h) the following: (g) DNA in which the nucleotide sequence contains the nucleotides 2080 to 2883 of sequence 11, or (h) DNA which can be recombined with a test subject in which the nucleotide sequence contains the nucleotides 2080 to 2883 of sequence 11 or a part of it under strict conditions and is transcribed for a protein whose function is dihydrotipicolinate reductase. 24. DNA which is transcribed for the protein specified in (I) or (J) below: (I) a protein in which the amino acid sequence of the order: 14, or (J) a protein in which the amino acid sequence of the order: 14 includes a substitution, deletion, addition, addition or reversal of one or more amino acids and has diaminopimylate decarboxylase function. 25. DNA pursuant to claim 24, which is defined as (i) or (j) the following: (i) DNA in which the nucleotide sequence contains the nucleotide sequences of nucleotides 751 to 1995 of sequence 13, or (j) DNA in which the nucleotide sequence contains the nucleotide sequences of nucleotides 751 to 1995 of sequence 13 or a portion of it under strict conditions and is transcribed for a protein that performs diaminopymylates decarboxylase function.