TH50271A - Non-transmitting pestivirus - Google Patents

Abstract

DC60 (26/01/43) The invention relates to vaccines used for the eradication or control of petivirus infections, particularly those used in pigs or ruminants. The invention produces petivirus-like nucleic acid particles and petivirus vaccines containing such nucleic acids or particles, which are capable of exhibiting an appropriate immune response without the ability to spread from vaccinated animals, thus avoiding the negative effect of viral spread. Ideally, such an immune response allows for serological diagnosis between vaccinated animals and animals spontaneously infected with petivirus. The invention relates to vaccines used for the eradication or control of petivirus infections, particularly those used in pigs or ruminants. The invention produces petivirus-like nucleic acid particles and petivirus vaccines containing such nucleic acids or particles, which are capable of exhibiting an appropriate immune response without the ability to spread from vaccinated animals, thus avoiding the negative effect of viral spread. Ideally, such an immune response allows for... Serological differentiation between vaccinated animals and animals infected with spontaneously occurring peptic viruses.

Claims (25)

1. Recombinant nucleic acid derived from pestivirus from which nucleic acid fragments encoding at least one pestivirus protein or a sufficiently large portion of a fragment associated with viral replication have been functionally deleted. Such nucleic acid provides for RNA replication in suitable cells and encoding at least one structural protein or at least one immunogenic portion of a structural protein. 2. The nucleic acid according to claim 1, which is RNA. 3. Nucleic acids according to claim 1 or 2, where the deletion of such function is located in the fragment encoding the shell protein. 4. Any nucleic acid under claims 1 through 3 in which the deletion of such function includes the immunogenic region of the said protein. 5. A nucleic acid pursuant to any one of claims 1 through 4, in which the structural protein is a shell protein that has a function, or at least one part that exhibits dominant immunogenicity. 6. The nucleic acid as per any one of claims 1 through 5 shall, in addition to a portion, contain non-pestivirus nucleic acid fragments. 7. Nucleic acids under claim 6, in which the non-pestiviral fragments are derived from pathogens or cytokines. 8. Nucleic acids as provided by any of Recollections 1 through 7, in which such cell contains nucleic acid synthesizers that encode at least a pestivirus protein or a sufficient portion of such proteins associated with viral propagation. 9. A cell containing a nucleic acid synthesizer that encoding at least one pestivirus protein or a sufficient fraction of a protein associated with viral replication, and capable of allowing the pestivirus protein or a sufficient fraction of such protein to be encapsulated in a pestivirus-like particle. 10. Cells commending claim 8 in which the pectinite protein, or a sufficient proportion of such proteins associated with viral replication, are stably expressed. 11. A method for the acquisition of pestivirus-like particles consisting of infection of a cell according to claim 9 or 10 with a nucleic acid according to claims 1 through 8, followed by the provision of such nucleic acid to be transfected in such cell, followed by the provision of a transfected nucleic acid part of a particle containing at least a pestivirus protein or a part of such protein derived from such cell, and followed by the collection of such particle. 12. The use of nucleic acids under any one of claims 1 through 8 in the production of pestivirus-like particles. 13. Pestivirus-like particles can be obtained using the methods specified in Patent 11. 14. A pestivirus-like particle containing a nucleic acid, as described in any one of claims 1 through 8. 15. A method for the determination of non-dispersing pestivirus vaccines consisting of the determination of a large number of particles by the method according to claim 11 and the preparation of a suspension of such particles in a suitable diluent. 16. The method under claim 14 also includes the combination of the aforementioned suspension with the additives. 17. A non-transmissible petrivirus vaccine may be available by means of claims 15 or 16. 18. A vaccine containing any one of the nucleic acids as per claims 1 through 8, or particles as per claim 13, and a diluent. 19. The vaccines under claim 18 also contain additives. 20. Any vaccine under claims 18 to 19 whose nucleic acid is derived from a pestivirus strain, such as the C-19 virus strain. 21. Any vaccine under claims 18 to 19 whose nucleic acid is derived from the classic swine fever virus. 22. Any vaccine under claims 18 to 19 whose nucleic acid is derived from bovine viral diarrhea. 23. Any vaccine under claims 16 through 19 whose nucleic acid is derived from a border virus. 24. Methods for controlling and/or eradicating pestivirus infection, which include vaccinating at least one animal with one of the vaccines specified in claims 17 through 23. 25. Methods for the control and/or eradication of pestivirus infection consisting of testing animals vaccinated with any of the vaccines under claims 17 through 23 for the appearance of spontaneously occurring pestivirus-specific antibodies.