TH49484B - Acid production method L-glutamic by fermentation obtained with precipitation. - Google Patents

Abstract

DC60 (08/02/56) Microorganisms capable of metabolizing carbon sources at specific pH in liquid culture medium. Contains L-glutamic acid at saturation concentration and carbon source and has the ability to accumulate L-glutamic acid. in quantities that exceed the dose corresponding to the intensity saturated in liquid culture medium at that pH; and a method for the production of L-glutamic acid by fermentation consisting of culturing microorganisms in liquid culture medium whose pH is adjusted to the pH in which L-glutamic acid is precipitated, to Production and accumulation of L-glutamic acid and L-glutamic acid precipitate in culture medium. Microorganisms which can metabolize carbon sources at specific pH in liquid culture medium Contains L-glutamic acid at saturation concentration and carbon source and has the ability to accumulate L-glutamic acid. in quantities that exceed the dose corresponding to the intensity saturated in liquid culture medium at that pH; and a method for the production of L-glutamic acid by fermentation consisting of culturing microorganisms in liquid culture medium whose pH is adjusted to pH in which L-glutamic acid is precipitated, to Production and accumulation of L-glutamic acid and L-glutamic acid precipitate in culture medium.

Claims (2)

Disclaimer (all) which will not appear on the announcement page: Amendment 12/3/2015 1. Methods for the production of al-glutamic acid by fermentation. Which consists of aquaculture A plant in a liquid medium in which the pH is adjusted to the pH where L-Glutamic acid is precipitated to produce and accumulate L-glutamic acid and acid coagulant. Al-glutamic acid in a carbon source medium with a pH of 3.0 to 5.0, whereby microorganisms can be metered with a carbon source at pH 3.0 to 5.0 in a liquid medium. Acidic L-glutamic acid at saturated concentrations and carbon sources and has the ability to accumulate excess L-Glutamic acid, the amount corresponding to the saturation concentration in it. 2. The method according to claim 1, whereby microorganisms have one or more of the following characteristics: (a) microorganisms are dominated by the enzyme that catalyzes for synthesis. And (b) microorganisms were reduced in or impaired in enzyme function that catalyzed branching reactions from The biosynthetic pathway of L-glutamic acid and the production of compounds other than L-glutamic acid 3. Method according to claim 2 whereby enzymes catalyzes for biosynthesis L-glutamic acid is at least one chosen from citrate syntase, phosphoenol pyruvate carboxylase and glutamate dehydration. Drogenase 5. Method according to any one of claims 1 to 4 where the microbes belong to the genus Enterobacter 6. Method according to claim 5 where the microorganisms are Enterobacter agglomerans 7. Method According to claim 6, where the microorganism is Enterobacter agglomerans (AJ13601) FERM BP-7207 ------------------------------ --------------- 1. Method for L-Glutamic Acid Production by Fermentation Which consists of Inorganic mediums in liquid culture medium in which the pH is adjusted to the pH where L-Glutamic acid is precipitated to produce and accumulate L-glutamic acid and acid coagulant. L-glutamic acid in culture media that Contains a carbon source with a pH of 3.0 to 5.0, whereby microorganisms can be metered with a carbon source at pH 3.0 to 5.0 in a culture medium. Liquid type containing acid L-glutamic acid at saturated concentration and carbon source and has the ability to accumulate acids. An excess of L-Glutamic content, the amount corresponding to the satiety concentration. The presence of microorganisms in the liquid culture medium at pH. 2. Method of claim 1, where microorganisms can 3. Methods of claim 1 or 2, where pH is not greater than 5.0 4. Methods of either claim 1 to 3, where the microorganisms have At least one style Specific to the following: (a) the microorganisms that are enriched with enzyme reactions Catalyzes for the synthesis of L-glutamic acid; And (b) reduced or impaired microorganisms in terms of the enzyme-catalyzed activity. Pruning from the way Bio-synthesis of L-glutamic acid And the creation of compounds other than L-Glutamic acid 5. Method according to claim 4, where enzymes that accelerate The reaction for the synthesis of at least one L-Glutamic acid was selected from citrate syntase, phosphoenol pyruvate carboxylase and glutamate. Dehydrogenase 6. Method according to claim 4 or 5, where the enzyme that Accelerate the reaction of branching from the pathway. Biosynthesis of L-glutamic acid and the formation of compounds other than L-glutamic acid ie alpha-ketoglutarate dehydrogenase. 7. One of the methods of Clause 1-6, where microorganisms belong to the Enterobacter family 8. Method according to claim 7, where microorganisms are Enterobacter agglomerans 9. Method according to claim 8, where microorganisms reproduce. That causes the secretion of substances Less viscous cells When compared with the original breed When cultured in the body Medium containing zaccharides 1 0. Method for screening microorganisms suitable for L-glutamic acid is produced by fermentation with L-glutamic acid precipitation. Mick in a liquid medium containing a sample that contains microorganisms into an acidic medium containing L-glutamic acid. Saturation and Carbon Source Concentration and Line Selection Varieties that can use carbon source food 1 1. Method according to claim 10, where strains capable of growing in the medium were selected as those that could use the carbon source food 1. 2. Method according to claim 10 or 11, where the pH of the medium is not more than 5.0.