TH40135A - A new method for combining arm-specific genes in mammalian cells through homologous and vector recombination for such actions. - Google Patents

Abstract

DC60 (20/05/41) A method has been described to obtain the desired binding of DNA specific arms at the target arm in the cell. Mammals by Through recombination, homologous This method provides selection of cell lines. That can be reproduced where the desired DNA is concentrated in place Arms that are sensitive to the defined implants By being marked before with a plasmid of the mark This method is especially suitable for Produces mammalian cell strands that drive high levels of mammalian proteins, especially immunoglobulins, and also combine new vectors and vectors. For use in Such copying as well A method has been described to obtain a specific combination of arms of DNA required at the target arm in mammalian cells by Through recombination, homologous This method provides selection of cell lines. That can be reproduced where the desired DNA is concentrated in place Arms that are sensitive to the defined implants By being marked first With plasmid body mark This method is especially suitable for Produce mammalian cell lines, which drive animal proteins. Mammals at high altitudes, especially immunoglobulins, and also provides new and combined vectors for use in the Such copying as well

Claims (1)

1. A method for inserting the required DNA at the target arm in the mammalian genome. It consists of the following steps: (i) mammalian cell transplantation with first plasmid ("plasmid mark") with the following sequence: (a) DNA region. At heterologas with animal cell genomes Mammals, which when combined with the mammalian cell genome Will provide a single arm for the homologous recombination; (b) The DNA segment coded the first segment of the marker protein. Can be selected; And (c) at least one selectable marker DNA of the tag cell: