TH35877B - Human growth hormone production process in yeast Pichia pastoris - Google Patents

Abstract

DC60 (March 28, 2013) The process of producing synthetic human growth hormone involves culturing and inducing production using methanol. The yeast Pichia pastoris, modified to have a base sequence controlling the synthesis of human growth hormone (hGH), is linked to the yeast genome to produce synthetic hGH protein. This synthetic hGH base sequence is linked to a base sequence that aids in secretion (secretion signaling). Improving hGH production in Pichia pastoris results in a product without the fMet component at the amino terminus, preventing negative responses when used for treatment. Furthermore, the product is secreted, reducing the purification process, resulting in higher quality and higher production quantities. This synthesized hGH protein is 22 kDa in size and has the same N-terminus as human growth hormone, making it potentially suitable for effective treatment of diseases requiring hGH. The process of producing synthetic human growth hormone involves culturing... The production of hGH is induced using methanol by inoculating the yeast Pichia pastoris, which has been modified to have a base sequence controlling the synthesis of human growth hormone (hGH). This sequence is linked to the yeast genome to produce synthetic hGH protein. The base sequence of synthetic hGH is linked to the base sequence that controls the secretion signal. Modification of hGH production in Pichia pastoris results in a product without the fMet component at the amino terminus, thus preventing negative responses when used for treatment. Furthermore, the product is secreted, reducing the purification process, resulting in higher quality and higher production quantities. This synthesized hGH protein is 22 kDa in size and has an N-terminus similar to human growth hormone, making it potentially suitable for effective treatment of diseases requiring hGH.

Claims (4)

1. The human growth hormone production process in the yeast Pichia pastoris containing Yeast cultivation with hGH gene synthesized in cells Induction of hormone production on the growth of Synthetic human The characteristic synthetic human growth hormone precipitation and purification are At least one copy of the yeast containing the synthetic hGH gene shown in Figure 1 was fed in the genome-inducing diet containing 3% (v / v) of methanol for 3 days. By adding pure methanol Every day, the final concentration of methanol is 3% of the feed amount. It then stores the vitreous, which contains synthetic human growth hormones. 2. A synthetic hGH nucleotide sequence with the use of codon suitable for protein synthesis in yeast is shown in Figure 1. The top 20 amino acid sequences of synthetic hGH have been designed specifically for yeast-specific codon use. 3. Plasmid used to express the hGH gene in yeast as shown in Figure 2 containing the baseline. Synthesized as claim 2, connected to the plasmid for the expression of yeast Pichia pastoris. 4. Yeast P. pastoris, modified to contain plasmids from claim 3, is linked to the unique yeast genome: Can produce hormones related to human growth And is secreted outside the 22 kDa cell with amino endings. It's like human growth hormones.