TH33833B - Methods for Microbial Production - Google Patents

Abstract

DC60 (28/09/44) A method for the production of target substances using microorganisms consisting of microbial cultures in a medium for the production and accumulation of target substances in the medium and target substance collection, where the microorganisms are generated from parent strains of microorganisms with high-energy efficient respiratory chain pathways and low-energy efficient respiratory chain pathways, and the microorganisms are mutant or recombinant strains with one or both of the following characteristics: (A) the high-energy efficient respiratory chain pathway is dominant, (B) the low-energy efficient respiratory chain pathway is deficient. A method for the production of target substances using microorganisms consisting of microbial cultures in a medium for the production and accumulation of target substances in the medium and target substance collection, where the microorganisms are generated from parent strains of microorganisms. Microorganisms with high-energy efficient respiratory chain pathways and low-energy efficient respiratory chain pathways are respiratory chain pathways, and microorganisms are mutant strains or genetically recombinant strains that exhibit one or both of the following characteristics: (A) the high-energy efficient respiratory chain pathway is dominant, (B) the low-energy efficient respiratory chain pathway is deficient.

Claims (9)

1. Methods for target meat production using microorganisms consisting of culture. Microorganisms in the medium to produce And accumulate the target material in the medium And target material collection, whereby microorganisms are generated from the parent species of Microorganisms with high energy efficient respiratory chain and low energy efficient respiratory chain pathways are the respiratory chain, and microorganisms are genetically mutated or recombined strains. That looks either Or both: (A) the high-energy efficient respiratory chain pathway is highlighted, (B) the low-energy efficient respiratory chain path is impaired. 2. Method for the production of target material according to claim 1, where the respiratory chain pathway Energy efficient High was distinguished by increasing the number of replication copies of transcribed genes for the enzymes involved in the respiratory chain or the transformation of the gene expression control sequence. 3. Methods for the production of the target material according to claim 1 or 2, where the chain path Effective breathing Low energy is impaired by the rupture of the transcribed gene for the enzyme involved in the respiratory chain. 4. Methods for producing the target material according to any of the 1 to 3 rights, whereby the enzymes of the respiratory chain are High energy efficiency includes SoxM type oxidase, bc1, NDH-1 or two or three of them. 5. Methods for the production of target meat according to any of the claims 1 to 4, where the respiratory chain enzymes are Low energy efficiency Including cytochrome bd type oxidase, NDH-II, or both of them. 6. Methods for producing the target material according to any one of claims 1 to 5, where the function of SoxM-type oxidase is dominated and NDH-II is impaired in the capacity Tree 7. Method for the production of target material according to any one of claims 1 through 6, where SoxM oxidase is a cytochrome bo cytochrome oxidase. 8. Methods for producing target material according to any of Claims 1 through 7, whereby microorganisms are bacteria of the family Escherichia or chorine form bacteria. 9. Methods for the production of the target material according to any of the claims 1 to 8, where the target material is L-amino acid or nucleic acid.